MD04G1037800.v1.1

Nucleotide-sugar transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008649
Physical Location & Seq
Forward (+)
4210161 .. 4219287
9127 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1037800.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGAATAAAGGGATGATAGAATGTAGTGTATGCCATTCAAAGATTGTTGCCCCGAACAGTAAAACTTTCTCCAGGGCCTATGACCGCCACAAGAGCAGGGTGTCGTCCAAGCAACGTGTTCTCAATGTCCTCTTGGTCGTTGGTGATTGTATGCTTGTCGGTTTACAGCCTATTCTGGTTTATATGTCCAAGGTCGATGGAAAATTCGAGTTTAGCCCGATTAGTGTAAACTTTTTGACAGAGGCTGCAAAGGTTCTATTTGCTGTCGTTATGCTATTGTTGCAGGCTAGGAATCAGAAAGTAGGGGAGAAACCTCTTCTCTCAATTTCCACATTCACGCAGGCAGCTCGGAATAATGTGCTTCTTGCTGTTCCAGCATTTCTTTATGCTATTAATAACTATTTAAAGTTCACCATGCAGCTATATTTCAATCCTGCAACTGTGAAGATGCTGAGCAATTTGAAGGTGTTGGTGATTGCTGTTTTGTTGAAAATGATAATGAAGCGCCGGTTTTCAATAATTCAGTGGGAAGCCCTTGCTTTGTTGCTCATCGGGATTAGTGTAAATCAGTTACGATCTTTACCCGAGGGTACTATTGCTTTAGGTCTTCCAGTTTCAACAGGCGCATACATCTACACATTGATCTTTGTAACAGTTCCATCGTTGGCCTCTGTCTATAATGAGTATGCTCTGAAGAGCCAATATGACACAAGCATATACCTCCAGAACTTATTTTTATACGGGTATGGTGCGATATTCAACTTTCTAGGAATATTGGTAATGGCCATTATCAAAGGTCCAGGTAGCTTTGATATCCTTCATGGTCATTCAAGAGCTACAATGTTTCTGATAGCTAACAACGCAGCACAAGGAATTCTGTCCTCTTTTTTCTTCAAATATGCAGATACAATTCTAAAGAAGTACTCGTCCACAGTGGCCACTATCTTCACAGGCATAGCATCTGCTTTGCTGTTTGGTCACAAATTGACTATGAACTTTGTTTTAGGAATTTCTATTGTCTTCATTTCGATGCACCAGTTCTTTTCTCCCATTTCAAAACTCAAGGAGGAGGAACAAAATGGGAAGCTGGAAATGATAGATGTCGATGATAAACACAGGTCAAAGGATTCCTTTATTAATATGGCAGCAGGGGCAAATGAAGAGTCACCGACCAGGCCCTGA

Protein Analysis

394

Amino Acids

43.66

Weight (kDa)

9.59

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nuc_sug_transp PF04142 59 - 344 3.5e-32 Nucleotide-sugar transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 85
AcoI YGGCCR 2 cut(s) 783, 936
AcsI RAATTY 3 cut(s) 203, 873, 1008
AcuI CTGAAG 1 cut(s) 713
AfaI GTAC 2 cut(s) 592, 923
AflIII ACRYGT 1 cut(s) 115
AjnI CCWGG 3 cut(s) 71, 799, 1172
AluBI AGCT 6 cut(s) 347, 421, 807, 836, 854, 1087
AluI AGCT 6 cut(s) 347, 421, 807, 836, 854, 1087
AlwNI CAGNNNCTG 2 cut(s) 245, 1179
Ama87I CYCGRG 1 cut(s) 584
AoxI GGCC 5 cut(s) 75, 666, 783, 936, 1175
ApeKI GCWGC 5 cut(s) 245, 344, 418, 863, 1145
ApoI RAATTY 3 cut(s) 203, 873, 1008
ArsI GACNNNNNNTTYG 2 cut(s) 1148, 1180
AseI ATTAAT 2 cut(s) 393, 1137
AspLEI GCGC 2 cut(s) 507, 626
AspS9I GGNCC 3 cut(s) 75, 797, 1176
AsuHPI GGTGA 4 cut(s) 155, 403, 484, 1158
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 1 cut(s) 797
BalI TGGCCA 2 cut(s) 785, 938
BbsI GAAGAC 2 cut(s) 599, 1012
BbvI GCAGC 5 cut(s) 232, 356, 430, 875, 1157
BccI CCATC 2 cut(s) 191, 667
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 3 cut(s) 73, 801, 1174
BfaI CTAG 2 cut(s) 288, 767
BfoI RGCGCY 1 cut(s) 508
BisI GCNGC 5 cut(s) 246, 345, 419, 864, 1146
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 5 cut(s) 247, 346, 420, 865, 1147
BmcAI AGTACT 1 cut(s) 923
Bme1390I CCNGG 3 cut(s) 73, 801, 1174
Bme18I GGWCC 1 cut(s) 797
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 3 cut(s) 75, 797, 1176
BmrFI CCNGG 3 cut(s) 73, 801, 1174
BmsI GCATC 3 cut(s) 438, 968, 1020
BpiI GAAGAC 2 cut(s) 599, 1012
BpmI CTGGAG 2 cut(s) 55, 707
Bpu1102I GCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 1046
BsaJI CCNNGG 3 cut(s) 72, 189, 585
Bse118I RCCGGY 1 cut(s) 507
Bse1I ACTGG 2 cut(s) 611, 1036
BseBI CCWGG 3 cut(s) 73, 801, 1174
BseDI CCNNGG 3 cut(s) 72, 189, 585
BseGI GGATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 2 cut(s) 611, 1036
BseRI GAGGAG 1 cut(s) 1082
BseXI GCAGC 5 cut(s) 232, 356, 430, 875, 1157
BshFI GGCC 5 cut(s) 77, 668, 785, 938, 1177
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 1 cut(s) 508
BsnI GGCC 5 cut(s) 77, 668, 785, 938, 1177
BsoBI CYCGRG 1 cut(s) 584
Bsp143I GATC 2 cut(s) 575, 642
Bsp1720I GCTNAGC 1 cut(s) 452
BspACI CCGC 1 cut(s) 85
BspANI GGCC 5 cut(s) 77, 668, 785, 938, 1177
BspCNI CTCAG 1 cut(s) 444
BspQI GCTCTTC 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 507
BsrI ACTGG 2 cut(s) 611, 1036
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 3 cut(s) 72, 189, 585
BssMI GATC 2 cut(s) 575, 642
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 73, 801, 1174
Bst4CI ACNGT 4 cut(s) 59, 442, 655, 934
Bst6I CTCTTC 3 cut(s) 321, 689, 1155
BstC8I GCNNGC 2 cut(s) 285, 342
BstDEI CTNAG 1 cut(s) 452
BstF5I GGATG 1 cut(s) 18
BstH2I RGCGCY 1 cut(s) 508
BstHHI GCGC 2 cut(s) 507, 626
BstKTI GATC 2 cut(s) 578, 645
BstMBI GATC 2 cut(s) 575, 642
BstMWI GCNNNNNNNGC 5 cut(s) 93, 280, 374, 860, 1151
BstNI CCWGG 3 cut(s) 73, 801, 1174
BstSCI CCNGG 3 cut(s) 71, 799, 1172
BstV1I GCAGC 5 cut(s) 232, 356, 430, 875, 1157
BstV2I GAAGAC 2 cut(s) 599, 1012
BsuRI GGCC 5 cut(s) 77, 668, 785, 938, 1177
BtsCI GGATG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 530, 939
Cac8I GCNNGC 2 cut(s) 285, 342
CaiI CAGNNNCTG 2 cut(s) 245, 1179
CfoI GCGC 2 cut(s) 507, 626
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 3 cut(s) 75, 797, 1176
Csp6I GTAC 2 cut(s) 591, 922
CviAII CATG 2 cut(s) 415, 821
CviQI GTAC 2 cut(s) 591, 922
DdeI CTNAG 1 cut(s) 452
DpnI GATC 2 cut(s) 577, 644
DpnII GATC 2 cut(s) 575, 642
DraI TTTAAA 1 cut(s) 405
EaeI YGGCCR 2 cut(s) 783, 936
Eam1104I CTCTTC 3 cut(s) 321, 689, 1155
EarI CTCTTC 3 cut(s) 321, 689, 1155
Eco130I CCWWGG 1 cut(s) 189
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 797
Eco57I CTGAAG 1 cut(s) 713
Eco88I CYCGRG 1 cut(s) 584
EcoO109I RGGNCCY 2 cut(s) 75, 1176
EcoRI GAATTC 1 cut(s) 873
EcoRII CCWGG 3 cut(s) 71, 799, 1172
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 2 cut(s) 418, 824
FalI AAGNNNNNCTT 2 cut(s) 1115, 1147
FatI CATG 2 cut(s) 414, 820
Fnu4HI GCNGC 5 cut(s) 246, 345, 419, 864, 1146
FokI GGATG 1 cut(s) 25
Fsp4HI GCNGC 5 cut(s) 246, 345, 419, 864, 1146
FspBI CTAG 2 cut(s) 288, 767
GlaI GCGC 2 cut(s) 506, 625
GluI GCNGC 5 cut(s) 246, 345, 419, 864, 1146
GsuI CTGGAG 2 cut(s) 55, 707
HaeII RGCGCY 1 cut(s) 508
HaeIII GGCC 5 cut(s) 77, 668, 785, 938, 1177
HapII CCGG 1 cut(s) 508
HhaI GCGC 2 cut(s) 507, 626
Hin1II CATG 2 cut(s) 418, 824
Hin6I GCGC 2 cut(s) 505, 624
HinP1I GCGC 2 cut(s) 505, 624
HinfI GANTC 3 cut(s) 292, 1127, 1163
HpaII CCGG 1 cut(s) 508
HphI GGTGA 4 cut(s) 155, 403, 484, 1158
Hpy166II GTNNAC 4 cut(s) 164, 229, 411, 930
Hpy188I TCNGA 4 cut(s) 297, 351, 693, 849
Hpy188III TCNNGA 3 cut(s) 553, 724, 831
Hpy8I GTNNAC 4 cut(s) 164, 229, 411, 930
HpyAV CCTTC 2 cut(s) 457, 827
HpyCH4III ACNGT 4 cut(s) 59, 442, 655, 934
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 6 cut(s) 248, 283, 418, 437, 902, 1033
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 280, 374, 860, 1151
HpyF3I CTNAG 1 cut(s) 452
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 2 cut(s) 418, 824
HspAI GCGC 2 cut(s) 505, 624
Kzo9I GATC 2 cut(s) 575, 642
LguI GCTCTTC 1 cut(s) 689
Lsp1109I GCAGC 5 cut(s) 232, 356, 430, 875, 1157
LweI GCATC 3 cut(s) 438, 968, 1020
MaeI CTAG 2 cut(s) 288, 767
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 4 cut(s) 570, 649, 977, 1164
MalI GATC 2 cut(s) 577, 644
MboI GATC 2 cut(s) 575, 642
MboII GAAGA 8 cut(s) 308, 457, 599, 706, 883, 937, 1012, 1172
MlsI TGGCCA 2 cut(s) 785, 938
MluCI AATT 8 cut(s) 203, 324, 457, 519, 873, 909, 983, 1008
MluNI TGGCCA 2 cut(s) 785, 938
MlyI GAGTC 1 cut(s) 1172
MnlI CCTC 9 cut(s) 140, 235, 324, 580, 679, 731, 892, 1060, 1063
Mox20I TGGCCA 2 cut(s) 785, 938
MscI TGGCCA 2 cut(s) 785, 938
MseI TTAA 3 cut(s) 393, 404, 1137
MslI CAYNNNNRTG 1 cut(s) 1028
Msp20I TGGCCA 2 cut(s) 785, 938
MspI CCGG 1 cut(s) 508
MspR9I CCNGG 3 cut(s) 73, 801, 1174
MvaI CCWGG 3 cut(s) 73, 801, 1174
MwoI GCNNNNNNNGC 5 cut(s) 93, 280, 374, 860, 1151
NdeII GATC 2 cut(s) 575, 642
NlaIII CATG 2 cut(s) 418, 824
NmuCI GTSAC 2 cut(s) 977, 1164
PciSI GCTCTTC 1 cut(s) 689
PfeI GAWTC 2 cut(s) 292, 1127
PkrI GCNGC 5 cut(s) 247, 346, 420, 865, 1147
PleI GAGTC 1 cut(s) 1171
PpsI GAGTC 1 cut(s) 1171
PshBI ATTAAT 2 cut(s) 393, 1137
Psp6I CCWGG 3 cut(s) 71, 799, 1172
PspGI CCWGG 3 cut(s) 71, 799, 1172
PspPI GGNCC 3 cut(s) 75, 797, 1176
PstNI CAGNNNCTG 2 cut(s) 245, 1179
RsaI GTAC 2 cut(s) 592, 923
RsaNI GTAC 2 cut(s) 591, 922
RseI CAYNNNNRTG 1 cut(s) 1028
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 3 cut(s) 393, 404, 1137
SatI GCNGC 5 cut(s) 246, 345, 419, 864, 1146
Sau3AI GATC 2 cut(s) 575, 642
Sau96I GGNCC 3 cut(s) 75, 797, 1176
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 1 cut(s) 1172
ScrFI CCNGG 3 cut(s) 73, 801, 1174
SfaNI GCATC 3 cut(s) 438, 968, 1020
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 1 cut(s) 1061
SmoI CTYRAG 1 cut(s) 1061
Sse9I AATT 8 cut(s) 203, 324, 457, 519, 873, 909, 983, 1008
SsiI CCGC 1 cut(s) 85
SspI AATATT 1 cut(s) 774
SspMI CTAG 2 cut(s) 288, 767
StyD4I CCNGG 3 cut(s) 71, 799, 1172
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 4 cut(s) 59, 442, 655, 934
TaiI ACGT 1 cut(s) 118
TaqI TCGA 4 cut(s) 195, 207, 1028, 1104
TasI AATT 8 cut(s) 203, 324, 457, 519, 873, 909, 983, 1008
TatI WGTACW 1 cut(s) 921
TfiI GAWTC 2 cut(s) 292, 1127
Tru1I TTAA 3 cut(s) 393, 404, 1137
Tru9I TTAA 3 cut(s) 393, 404, 1137
TscAI CASTG 2 cut(s) 530, 939
TseFI GTSAC 2 cut(s) 977, 1164
TseI GCWGC 5 cut(s) 245, 344, 418, 863, 1145
Tsp45I GTSAC 2 cut(s) 977, 1164
TspDTI ATGAA 6 cut(s) 17, 515, 809, 1007, 1012, 1173
TspRI CASTG 2 cut(s) 530, 939
VpaK11BI GGWCC 1 cut(s) 797
VspI ATTAAT 2 cut(s) 393, 1137
XapI RAATTY 3 cut(s) 203, 873, 1008
XspI CTAG 2 cut(s) 288, 767
ZrmI AGTACT 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.