AT3G60300

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009599
Physical Location & Seq
Forward (+)
22285138 .. 22287548
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60300.2

Sequence Viewer

Length: 1101 bp
ATGACGGAGGAAGAGGTAGCTATGGAAGTGGAAGCTCTAGAAGCAGTTTACAGCGAAGACTGCGTTATACTGGATTCGTATCCTCCTCATCTTCACCTCCATATCAAGCCCCGTACCGCCGAAATCTCTTCTCAGCAGTTTGTGGAAGCCGTTGTTAGATTCCAAGCAGGTTCTAAGTATCCAGATGAACCACCTCGGATTAGTCTGATTGAATCCAAGGGTCTTGACGAGCAAAGGCAAAAGCTTTTGATTGGCATAGTGCAAGAGAAAGCTTCTCAACTATCATCTTCTTTAATGCTTGTAGAACTTTGTGAGGAAGCAGTGGAGAGGCTCACAATTATGAATCACCCGGAGGGAGATTGTCCACTGTGTTTGTATCCGTTATTCCCTGAGGATGGCGGGAGTAAACAAATGCCATTTATGAAGCTGATGTCTTGTTTTCATTGTTTCCACTGTGAGTGCATCATTAGGTGGTGGAATTGGCTTCATGCACAGGAAGAGGCTGATTCGAAATCTGGTGACACTTTACATATGCGTAGAGGATCAACTGCTCGAGAAGGTAATTCTGGATCAGCAGATAAAAGCTTGGGAAACTGTCCAGTGTGCCGCAAAATTTTTCACTCGAGTGACATTGAACATGTTCTTGACTTAGTTGGTGCTCAATCATCTCTACAGGACTCTAGTTTAATACCAGGCAAGGACCAAGACCCGCTGCTCCAGTCAGATTCAGAGAACATAAGAAGAGAGCGGTTTGAGTCTATTCTAAAGACGCAGGAAGAAAAGGGTGGTTTGGTTCAACCGAAGAAAAACATATCGGTGGTGCCCGGTATGTATCTTCCTCCACCAGCTCATGCATCAACGTCACGAAACGAAGAAGAAGAAGCTGGTGAAAGCCAAGAACAAGGAGAAGAGGAACCCAAAGAAGCTGAATCAGAGACAAACTCTAGCAGCTCCACCAACAGGAGAGGTCGAGGGAGATGGAGAGGCAGAGGCAGAAGCAGAGGCCGGGGACCTACTGTCAATGAAAGGAAGCCGAATTCGCAAGATCCAAGAAAACCGACGAGACAATGGGTGCAGAGAGCCAGAGAAGGAGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWD

Protein Analysis

366

Amino Acids

41.22

Weight (kDa)

5.45

Isoelectric Point (pI)

67.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWD PF05773 4 - 127 1.6e-16 RWD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 820
AccBSI CCGCTC 1 cut(s) 748
AciI CCGC 5 cut(s) 117, 399, 607, 710, 748
AclWI GGATC 3 cut(s) 550, 577, 1040
AcsI RAATTY 2 cut(s) 612, 1036
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 2 cut(s) 395, 960
AflIII ACRYGT 1 cut(s) 637
AgsI TTSAA 3 cut(s) 212, 635, 797
AhdI GACNNNNNGTC 1 cut(s) 1016
AjnI CCWGG 1 cut(s) 691
AleI CACNNNNGTG 1 cut(s) 624
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 3 cut(s) 929, 1057, 1086
AlwI GGATC 3 cut(s) 550, 577, 1040
Ama87I CYCGRG 2 cut(s) 552, 622
AoxI GGCC 1 cut(s) 1003
ApeKI GCWGC 2 cut(s) 712, 948
ApoI RAATTY 2 cut(s) 612, 1036
Asp700I GAANNNNTTC 1 cut(s) 639
AspS9I GGNCC 2 cut(s) 700, 1010
AsuC2I CCSGG 3 cut(s) 350, 825, 1007
AsuHPI GGTGA 4 cut(s) 86, 338, 530, 899
AsuII TTCGAA 1 cut(s) 509
AvaI CYCGRG 2 cut(s) 552, 622
AvaII GGWCC 2 cut(s) 700, 1010
AxyI CCTNAGG 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 825
BanI GGYRCC 1 cut(s) 820
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 661
BbvI GCAGC 2 cut(s) 699, 960
BccI CCATC 2 cut(s) 389, 972
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 1 cut(s) 693
BciVI GTATCC 3 cut(s) 90, 189, 387
BcnI CCSGG 3 cut(s) 350, 825, 1007
BcoDI GTCTC 3 cut(s) 929, 1057, 1086
BfaI CTAG 3 cut(s) 38, 681, 945
BfmI CTRYAG 1 cut(s) 671
BfuAI ACCTGC 1 cut(s) 158
BfuI GTATCC 3 cut(s) 90, 189, 387
BisI GCNGC 3 cut(s) 607, 713, 949
BlsI GCNGC 3 cut(s) 608, 714, 950
Bme1390I CCNGG 4 cut(s) 350, 693, 825, 1007
Bme18I GGWCC 2 cut(s) 700, 1010
BmeRI GACNNNNNGTC 1 cut(s) 1016
BmeT110I CYCGRG 2 cut(s) 552, 622
BmgT120I GGNCC 2 cut(s) 700, 1010
BmiI GGNNCC 3 cut(s) 822, 915, 1011
BmrFI CCNGG 4 cut(s) 350, 693, 825, 1007
BmsI GCATC 2 cut(s) 471, 863
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 926, 958
BpmI CTGGAG 1 cut(s) 701
Bpu14I TTCGAA 1 cut(s) 509
BpuMI CCSGG 3 cut(s) 350, 825, 1007
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 3 cut(s) 194, 216, 1006
Bsc4I CCNNNNNNNGG 2 cut(s) 395, 960
Bse1I ACTGG 3 cut(s) 75, 599, 718
Bse21I CCTNAGG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 1 cut(s) 693
BseDI CCNNGG 3 cut(s) 194, 216, 1006
BseGI GGATG 1 cut(s) 400
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 2 cut(s) 395, 960
BseMII CTCAG 2 cut(s) 146, 381
BseNI ACTGG 3 cut(s) 75, 599, 718
BseRI GAGGAG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 2 cut(s) 699, 960
BsgI GTGCAG 1 cut(s) 1094
BshFI GGCC 1 cut(s) 1005
BshNI GGYRCC 1 cut(s) 820
BsiHKAI GWGCWC 1 cut(s) 661
BsiHKCI CYCGRG 2 cut(s) 552, 622
BsiSI CCGG 3 cut(s) 350, 825, 1006
BslFI GGGAC 1 cut(s) 1023
BslI CCNNNNNNNGG 2 cut(s) 395, 960
BsmAI GTCTC 3 cut(s) 929, 1057, 1086
BsmFI GGGAC 1 cut(s) 1023
BsnI GGCC 1 cut(s) 1005
BsoBI CYCGRG 2 cut(s) 552, 622
Bsp119I TTCGAA 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 661, 825
Bsp143I GATC 3 cut(s) 542, 569, 1045
BspACI CCGC 5 cut(s) 117, 399, 607, 710, 748
BspANI GGCC 1 cut(s) 1005
BspCNI CTCAG 2 cut(s) 145, 382
BspLI GGNNCC 3 cut(s) 822, 915, 1011
BspMI ACCTGC 1 cut(s) 158
BspPI GGATC 3 cut(s) 550, 577, 1040
BspT104I TTCGAA 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 820
BsrBI CCGCTC 1 cut(s) 748
BsrI ACTGG 3 cut(s) 75, 599, 718
BssECI CCNNGG 3 cut(s) 194, 216, 1006
BssMI GATC 3 cut(s) 542, 569, 1045
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 693
Bst4CI ACNGT 4 cut(s) 369, 455, 596, 1018
Bst6I CTCTTC 5 cut(s) 6, 133, 492, 736, 903
BstBI TTCGAA 1 cut(s) 509
BstDEI CTNAG 4 cut(s) 132, 174, 390, 649
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 3 cut(s) 545, 572, 1048
BstMAI GTCTC 3 cut(s) 929, 1057, 1086
BstMBI GATC 3 cut(s) 542, 569, 1045
BstMWI GCNNNNNNNGC 3 cut(s) 41, 60, 1039
BstNI CCWGG 1 cut(s) 693
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 4 cut(s) 348, 691, 823, 1005
BstSFI CTRYAG 1 cut(s) 671
BstSLI GKGCMC 1 cut(s) 825
BstV1I GCAGC 2 cut(s) 699, 960
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 1045
BstYI RGATCY 1 cut(s) 1045
Bsu36I CCTNAGG 1 cut(s) 390
BsuI GTATCC 3 cut(s) 90, 189, 387
BsuRI GGCC 1 cut(s) 1005
BtsCI GGATG 1 cut(s) 400
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 4 cut(s) 327, 365, 451, 606
BveI ACCTGC 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 700, 1010
CseI GACGC 1 cut(s) 778
Csp6I GTAC 1 cut(s) 114
CspCI CAANNNNNGTGG 2 cut(s) 354, 389
CviAII CATG 3 cut(s) 488, 638, 851
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 4 cut(s) 132, 174, 390, 649
DpnI GATC 3 cut(s) 544, 571, 1047
DpnII GATC 3 cut(s) 542, 569, 1045
DriI GACNNNNNGTC 1 cut(s) 1016
Eam1104I CTCTTC 5 cut(s) 6, 133, 492, 736, 903
Eam1105I GACNNNNNGTC 1 cut(s) 1016
EarI CTCTTC 5 cut(s) 6, 133, 492, 736, 903
Eco130I CCWWGG 1 cut(s) 216
Eco47I GGWCC 2 cut(s) 700, 1010
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 2 cut(s) 552, 622
EcoO109I RGGNCCY 1 cut(s) 1010
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 1 cut(s) 691
EcoT14I CCWWGG 1 cut(s) 216
EcoT22I ATGCAT 1 cut(s) 856
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 3 cut(s) 491, 641, 854
FaqI GGGAC 1 cut(s) 1023
FatI CATG 3 cut(s) 487, 637, 850
FauI CCCGC 2 cut(s) 392, 717
FauNDI CATATG 1 cut(s) 531
Fnu4HI GCNGC 3 cut(s) 607, 713, 949
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 3 cut(s) 607, 713, 949
FspBI CTAG 3 cut(s) 38, 681, 945
GluI GCNGC 3 cut(s) 607, 713, 949
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 1 cut(s) 1005
HapII CCGG 3 cut(s) 350, 825, 1006
HgaI GACGC 1 cut(s) 778
Hin1II CATG 3 cut(s) 491, 641, 854
HindIII AAGCTT 3 cut(s) 242, 270, 583
HinfI GANTC 9 cut(s) 74, 159, 212, 343, 506, 677, 725, 755, 929
HpaII CCGG 3 cut(s) 350, 825, 1006
HphI GGTGA 4 cut(s) 86, 338, 530, 899
Hpy166II GTNNAC 3 cut(s) 49, 365, 407
Hpy188I TCNGA 5 cut(s) 198, 207, 724, 730, 934
Hpy188III TCNNGA 7 cut(s) 38, 182, 224, 554, 567, 644, 864
Hpy8I GTNNAC 3 cut(s) 49, 365, 407
Hpy99I CGWCG 1 cut(s) 1063
HpyAV CCTTC 2 cut(s) 551, 1082
HpyCH4III ACNGT 4 cut(s) 369, 455, 596, 1018
HpyCH4IV ACGT 1 cut(s) 860
HpyCH4V TGCA 5 cut(s) 262, 462, 491, 854, 1075
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 60, 1039
HpyF3I CTNAG 4 cut(s) 132, 174, 390, 649
HpySE526I ACGT 1 cut(s) 860
Hsp92II CATG 3 cut(s) 491, 641, 854
Kzo9I GATC 3 cut(s) 542, 569, 1045
LmnI GCTCC 2 cut(s) 720, 956
Lsp1109I GCAGC 2 cut(s) 699, 960
LweI GCATC 2 cut(s) 471, 863
MaeI CTAG 3 cut(s) 38, 681, 945
MaeII ACGT 1 cut(s) 860
MaeIII GTNAC 3 cut(s) 518, 626, 861
MalI GATC 3 cut(s) 544, 571, 1047
MbiI CCGCTC 1 cut(s) 748
MboI GATC 3 cut(s) 542, 569, 1045
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 2 cut(s) 661, 825
MluCI AATT 5 cut(s) 336, 478, 562, 612, 1036
MlyI GAGTC 2 cut(s) 671, 764
Mph1103I ATGCAT 1 cut(s) 856
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 2 cut(s) 293, 686
MslI CAYNNNNRTG 3 cut(s) 338, 624, 815
MspA1I CMGCKG 1 cut(s) 712
MspI CCGG 3 cut(s) 350, 825, 1006
MspR9I CCNGG 4 cut(s) 350, 693, 825, 1007
MvaI CCWGG 1 cut(s) 693
MwoI GCNNNNNNNGC 3 cut(s) 41, 60, 1039
NciI CCSGG 3 cut(s) 350, 825, 1007
NdeI CATATG 1 cut(s) 531
NdeII GATC 3 cut(s) 542, 569, 1045
NlaIII CATG 3 cut(s) 491, 641, 854
NlaIV GGNNCC 3 cut(s) 822, 915, 1011
NmuCI GTSAC 3 cut(s) 518, 626, 861
NsiI ATGCAT 1 cut(s) 856
NspI RCATGY 1 cut(s) 641
NspV TTCGAA 1 cut(s) 509
OliI CACNNNNGTG 1 cut(s) 624
PaeR7I CTCGAG 2 cut(s) 552, 622
PciI ACATGT 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 7 cut(s) 74, 159, 212, 343, 506, 725, 929
PkrI GCNGC 3 cut(s) 608, 714, 950
PleI GAGTC 2 cut(s) 671, 763
PpsI GAGTC 2 cut(s) 671, 763
PpuMI RGGWCCY 1 cut(s) 1010
PscI ACATGT 1 cut(s) 637
Psp5II RGGWCCY 1 cut(s) 1010
Psp6I CCWGG 1 cut(s) 691
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 3 cut(s) 822, 915, 1011
PspPI GGNCC 2 cut(s) 700, 1010
PspPPI RGGWCCY 1 cut(s) 1010
PspXI VCTCGAGB 1 cut(s) 622
PsuI RGATCY 1 cut(s) 1045
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 3 cut(s) 338, 624, 815
SaqAI TTAA 2 cut(s) 293, 686
SatI GCNGC 3 cut(s) 607, 713, 949
Sau3AI GATC 3 cut(s) 542, 569, 1045
Sau96I GGNCC 2 cut(s) 700, 1010
SchI GAGTC 2 cut(s) 671, 764
ScrFI CCNGG 4 cut(s) 350, 693, 825, 1007
SduI GDGCHC 2 cut(s) 661, 825
SfaNI GCATC 2 cut(s) 471, 863
SfcI CTRYAG 1 cut(s) 671
Sfr274I CTCGAG 2 cut(s) 552, 622
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 2 cut(s) 700, 1010
SlaI CTCGAG 2 cut(s) 552, 622
SmiMI CAYNNNNRTG 3 cut(s) 338, 624, 815
SmlI CTYRAG 2 cut(s) 552, 622
SmoI CTYRAG 2 cut(s) 552, 622
Sse9I AATT 5 cut(s) 336, 478, 562, 612, 1036
SsiI CCGC 5 cut(s) 117, 399, 607, 710, 748
SspMI CTAG 3 cut(s) 38, 681, 945
StyD4I CCNGG 4 cut(s) 348, 691, 823, 1005
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 4 cut(s) 369, 455, 596, 1018
TaiI ACGT 1 cut(s) 863
TaqI TCGA 4 cut(s) 509, 553, 623, 970
TasI AATT 5 cut(s) 336, 478, 562, 612, 1036
TauI GCSGC 1 cut(s) 609
TfiI GAWTC 7 cut(s) 74, 159, 212, 343, 506, 725, 929
Tru1I TTAA 2 cut(s) 293, 686
Tru9I TTAA 2 cut(s) 293, 686
TscAI CASTG 4 cut(s) 327, 372, 458, 606
TseFI GTSAC 3 cut(s) 518, 626, 861
TseI GCWGC 2 cut(s) 712, 948
Tsp45I GTSAC 3 cut(s) 518, 626, 861
TspDTI ATGAA 6 cut(s) 201, 356, 431, 437, 476, 1038
TspGWI ACGGA 2 cut(s) 20, 369
TspRI CASTG 4 cut(s) 327, 372, 458, 606
VpaK11BI GGWCC 2 cut(s) 700, 1010
XapI RAATTY 2 cut(s) 612, 1036
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 1 cut(s) 641
XhoI CTCGAG 2 cut(s) 552, 622
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 3 cut(s) 38, 681, 945
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.