AT3G61050

Contains the following InterPro domains C2 membrane targeting protein (InterPro IPR018029), C2 calcium lipid-binding domain, CaLB (InterPro IPR008973)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012269
Physical Location & Seq
Forward (+)
22597216 .. 22601412
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G61050.1

Sequence Viewer

Length: 1533 bp
ATGGGTTTGATTTCTGGGATTCTGTTCGGGATTATCTTCGGCGTAGCTCTAATGGCTGGCTGGAGTCGTATGATGACTCACCGATCGTCCAAGCGAGTCGCCAAGGCAGTTGATATGAAACTACTAGGCTCTCTTAGCAGAGACGATTTGAAGAAAATTTGTGGTGATAATTTCCCTCAATGGATATCGTTTCCAGCATTTGAACAGGTCAAATGGCTGAATAAACTACTGAGCAAGATGTGGCCATATATTGCAGAGGCAGCAACAATGGTAATAAGAGATTCTGTTGAACCTCTGCTTGAAGATTACAGACCACCAGGAATTACTTCCCTGAAGTTCAGCAAATTGACGTTGGGTAACGTAGCACCAAAGATTGAAGGTATTCGTGTTCAAAGTTTCAAGGAAGGTCAAGTCACAATGGACGTTGATCTTCGATGGGGTGGTGATCCAAATATTGTTTTAGGTGTGACAGCACTTGTTGCCTCCATACCCATTCAGTTGAAGGATCTTCAAGTTTTCACAGTTGCACGTGTTATCTTTCAACTTGCGGATGAGATTCCTTGTATATCTGCTGTTGTTGTTGCTCTACTTGCTGAGCCAAAACCAAGAATTGATTACACTCTGAAGGCTGTTGGTGGAAGCTTAACAGCAATTCCTGGACTATCTGATATGATTGATGATACTGTTGATACTATCGTCAAAGACATGCTTCAGTGGCCTCATAGAATTGTTGTTCCCATTGGTGGTATTCCTGTTGATTTAAGTGATTTGGAGCTTAAGCCACAGGGAAAGCTTATAGTAACAGTTGTGAAAGCAACTAACTTGAAGAATAAGGAATTGATTGGAAAATCTGACCCTTATGCTACCATCTACATTCGTCCTGTATTCAAGTATAAAACAAAGGCAATCGAGAACAATCTGAATCCTGTCTGGGATCAAACATTCGAATTGATTGCAGAGGACAAAGAAACCCAGTCGCTCACTGTAGAGGTATTTGATAAAGACGTAGGTCAAGATGAGCGCCTTGGACTTGTGAAACTTCCCTTAAGCAGTTTGGAAGCCGGAGTTACAAAAGAACTGGAGCTAAATCTGTTGTCTTCACTTGATACTTTGAAAGTAAAAGATAAGAAAGATAGAGGAAGCATAACTCTTAAGGTACATTATCATGAGTTCAACAAAGAGGAGCAAATGGCTGCGTTGGAAGACGAGAAGAAGATCATGGAAGAAAGGAAGAGACTGAAGGAAGCAGGAGTGATAGGTAGCACAATGGATGCAGTCGGGATGGTGGGAAGTGGGCTCGGTGCTGGTGTAGGAATGGTTGGGACCGGTATTGGCACAGGAGTCGGATTGGTTGGAAGCGGTGTGAGCTCGGGTGTTGGGATGGTTGGTAGCGGTTTTGGAGCGGTCGGTAGTGGATTGAGCAAAGCAGGGAGATTTATGGGAAGAACAATCACAGGTCAGTCTAGCAAACGTAGTGGCTCCTCAACACCTGTGAATACCGTTCCAGAAAACGATGGTGCAAAACAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 SMP_LBD

Protein Analysis

510

Amino Acids

55.09

Weight (kDa)

8.22

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMP_LBD PF17047 68 - 247 3.5e-16 Synaptotagmin-like mitochondrial-lipid-binding domain
SMP_MUG190-like PF25669 68 - 134 1.4e-06 MUG190-like SMP domain
C2 PF00168 263 - 359 3.7e-24 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61050 AT3G61050
fragaria_vesca FvH4_7g12480 FvH4_7g12480
malus_domestica MD02G1198800.v1.1
prunus_persica Prupe.2G149400_v2.0.a1 Prupe.2G149400_v2.0.a1
pyrus_communis pycom02g16290
rosa_chinensis RchiOBHm_Chr1g0351321
rosa_laevigata RLG00000028415
rosa_multiflora Rmu_sc0004933.1_g000008 Rmu_sc0013758.1_g000001
rosa_roxburghii Rroxscaffold_4G00303880
rosa_rugosa Rorug01G0217600
rosa_samantha Rh1AG232000 Rh1BG202400 Rh1CG216800 Rh1DG229600
rosa_wichuraiana Rw1G020210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1403
AciI CCGC 4 cut(s) 548, 1359, 1392, 1403
AclWI GGATC 3 cut(s) 440, 513, 942
AcoI YGGCCR 1 cut(s) 242
AcsI RAATTY 1 cut(s) 156
AcuI CTGAAG 4 cut(s) 353, 644, 695, 1259
AcvI CACGTG 1 cut(s) 530
AfaI GTAC 1 cut(s) 1158
AfiI CCNNNNNNNGG 1 cut(s) 743
AflII CTTAAG 3 cut(s) 776, 1045, 1151
AflIII ACRYGT 1 cut(s) 529
AgeI ACCGGT 1 cut(s) 1325
AjnI CCWGG 2 cut(s) 316, 655
AluBI AGCT 6 cut(s) 47, 642, 775, 793, 1084, 1368
AluI AGCT 6 cut(s) 47, 642, 775, 793, 1084, 1368
Alw21I GWGCWC 1 cut(s) 1370
Alw26I GTCTC 2 cut(s) 135, 1228
AlwI GGATC 3 cut(s) 440, 513, 942
Ama87I CYCGRG 1 cut(s) 1369
AoxI GGCC 2 cut(s) 242, 716
ApeKI GCWGC 2 cut(s) 260, 1193
ApoI RAATTY 1 cut(s) 156
AsiGI ACCGGT 1 cut(s) 1325
Asp700I GAANNNNTTC 2 cut(s) 325, 381
AspLEI GCGC 1 cut(s) 1023
AspS9I GGNCC 1 cut(s) 1323
AsuHPI GGTGA 3 cut(s) 71, 176, 455
AsuII TTCGAA 1 cut(s) 945
AvaI CYCGRG 1 cut(s) 1369
AvaII GGWCC 1 cut(s) 1323
BaeI ACNNNNGTAYC 2 cut(s) 681, 714
BalI TGGCCA 1 cut(s) 244
BanII GRGCYC 2 cut(s) 1299, 1370
BbrPI CACGTG 1 cut(s) 530
BbsI GAAGAC 2 cut(s) 1089, 1209
Bbv12I GWGCWC 1 cut(s) 1370
BbvI GCAGC 2 cut(s) 272, 1180
BccI CCATC 5 cut(s) 429, 875, 1276, 1375, 1508
BcgI CGANNNNNNTGC 4 cut(s) 935, 969, 1324, 1358
BciT130I CCWGG 2 cut(s) 318, 657
BcoDI GTCTC 2 cut(s) 135, 1228
BfaI CTAG 2 cut(s) 125, 1464
BfmI CTRYAG 1 cut(s) 984
BfoI RGCGCY 1 cut(s) 1024
BfrI CTTAAG 3 cut(s) 776, 1045, 1151
BisI GCNGC 2 cut(s) 261, 1194
BlpI GCTNAGC 1 cut(s) 594
BlsI GCNGC 2 cut(s) 262, 1195
Bme1390I CCNGG 2 cut(s) 318, 657
Bme18I GGWCC 1 cut(s) 1323
BmeT110I CYCGRG 1 cut(s) 1369
BmgT120I GGNCC 1 cut(s) 1323
BmiI GGNNCC 2 cut(s) 1324, 1480
BmrFI CCNGG 2 cut(s) 318, 657
BmrI ACTGGG 1 cut(s) 967
BmsI GCATC 1 cut(s) 1261
BmuI ACTGGG 1 cut(s) 967
BoxI GACNNNNGTC 1 cut(s) 1008
BpiI GAAGAC 2 cut(s) 1089, 1209
BpmI CTGGAG 2 cut(s) 82, 1100
Bpu1102I GCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 945
BsaAI YACGTR 1 cut(s) 530
BsaJI CCNNGG 2 cut(s) 102, 1024
BsaWI WCCGGW 1 cut(s) 1325
Bsc4I CCNNNNNNNGG 1 cut(s) 743
Bse118I RCCGGY 1 cut(s) 1325
Bse1I ACTGG 2 cut(s) 973, 1083
BseBI CCWGG 2 cut(s) 318, 657
BseDI CCNNGG 2 cut(s) 102, 1024
BseGI GGATG 4 cut(s) 556, 1276, 1287, 1386
BseLI CCNNNNNNNGG 1 cut(s) 743
BseMII CTCAG 2 cut(s) 221, 585
BseNI ACTGG 2 cut(s) 973, 1083
BseRI GAGGAG 2 cut(s) 1196, 1471
BseXI GCAGC 2 cut(s) 272, 1180
Bsh1285I CGRYCG 2 cut(s) 86, 1407
BshFI GGCC 2 cut(s) 244, 718
BshTI ACCGGT 1 cut(s) 1325
BsiEI CGRYCG 2 cut(s) 86, 1407
BsiHKAI GWGCWC 1 cut(s) 1370
BsiHKCI CYCGRG 1 cut(s) 1369
BsiSI CCGG 2 cut(s) 1062, 1326
BslFI GGGAC 1 cut(s) 1336
BslI CCNNNNNNNGG 1 cut(s) 743
BsmAI GTCTC 2 cut(s) 135, 1228
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 1336
BsnI GGCC 2 cut(s) 244, 718
BsoBI CYCGRG 1 cut(s) 1369
Bsp119I TTCGAA 1 cut(s) 945
Bsp1286I GDGCHC 2 cut(s) 1299, 1370
Bsp143I GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
Bsp1720I GCTNAGC 1 cut(s) 594
BspACI CCGC 4 cut(s) 548, 1359, 1392, 1403
BspANI GGCC 2 cut(s) 244, 718
BspCNI CTCAG 2 cut(s) 222, 586
BspHI TCATGA 1 cut(s) 1165
BspLI GGNNCC 2 cut(s) 1324, 1480
BspPI GGATC 3 cut(s) 440, 513, 942
BspT104I TTCGAA 1 cut(s) 945
BspTI CTTAAG 3 cut(s) 776, 1045, 1151
BsrBI CCGCTC 1 cut(s) 1403
BsrFI RCCGGY 1 cut(s) 1325
BsrI ACTGG 2 cut(s) 973, 1083
BssAI RCCGGY 1 cut(s) 1325
BssECI CCNNGG 2 cut(s) 102, 1024
BssMI GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
BssT1I CCWWGG 2 cut(s) 102, 1024
Bst2UI CCWGG 2 cut(s) 318, 657
Bst4CI ACNGT 5 cut(s) 523, 685, 805, 985, 1501
Bst6I CTCTTC 1 cut(s) 1226
BstAFI CTTAAG 3 cut(s) 776, 1045, 1151
BstAPI GCANNNNNTGC 1 cut(s) 479
BstBAI YACGTR 1 cut(s) 530
BstBI TTCGAA 1 cut(s) 945
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 3 cut(s) 134, 230, 594
BstF5I GGATG 4 cut(s) 556, 1276, 1287, 1386
BstH2I RGCGCY 1 cut(s) 1024
BstHHI GCGC 1 cut(s) 1023
BstKTI GATC 6 cut(s) 86, 430, 448, 508, 937, 1218
BstMAI GTCTC 2 cut(s) 135, 1228
BstMBI GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
BstMCI CGRYCG 2 cut(s) 86, 1407
BstMWI GCNNNNNNNGC 7 cut(s) 53, 135, 260, 479, 590, 715, 1365
BstNI CCWGG 2 cut(s) 318, 657
BstNSI RCATGY 1 cut(s) 709
BstPAI GACNNNNGTC 1 cut(s) 1008
BstSCI CCNGG 2 cut(s) 316, 655
BstSFI CTRYAG 1 cut(s) 984
BstV1I GCAGC 2 cut(s) 272, 1180
BstV2I GAAGAC 2 cut(s) 1089, 1209
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BsuRI GGCC 2 cut(s) 244, 718
BtsCI GGATG 4 cut(s) 556, 1276, 1287, 1386
BtsIMutI CAGTG 2 cut(s) 719, 981
Cac8I GCNNGC 1 cut(s) 58
CciI TCATGA 1 cut(s) 1165
CfoI GCGC 1 cut(s) 1023
Cfr10I RCCGGY 1 cut(s) 1325
Cfr13I GGNCC 1 cut(s) 1323
Csp6I GTAC 1 cut(s) 1157
CspAI ACCGGT 1 cut(s) 1325
CspCI CAANNNNNGTGG 2 cut(s) 1456, 1491
CviAII CATG 3 cut(s) 706, 1166, 1219
CviQI GTAC 1 cut(s) 1157
DdeI CTNAG 3 cut(s) 134, 230, 594
DpnI GATC 6 cut(s) 85, 429, 447, 507, 936, 1217
DpnII GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
EaeI YGGCCR 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 1226
EarI CTCTTC 1 cut(s) 1226
Ecl136II GAGCTC 1 cut(s) 1368
Eco130I CCWWGG 2 cut(s) 102, 1024
Eco24I GRGCYC 2 cut(s) 1299, 1370
Eco32I GATATC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 1323
Eco53kI GAGCTC 1 cut(s) 1368
Eco57I CTGAAG 4 cut(s) 353, 644, 695, 1259
Eco72I CACGTG 1 cut(s) 530
Eco88I CYCGRG 1 cut(s) 1369
EcoICRI GAGCTC 1 cut(s) 1368
EcoRII CCWGG 2 cut(s) 316, 655
EcoRV GATATC 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 102, 1024
EcoT38I GRGCYC 2 cut(s) 1299, 1370
ErhI CCWWGG 2 cut(s) 102, 1024
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 3 cut(s) 709, 1169, 1222
FalI AAGNNNNNCTT 2 cut(s) 693, 725
FaqI GGGAC 1 cut(s) 1336
FatI CATG 3 cut(s) 705, 1165, 1218
Fnu4HI GCNGC 2 cut(s) 261, 1194
FokI GGATG 4 cut(s) 563, 1283, 1294, 1393
FriOI GRGCYC 2 cut(s) 1299, 1370
Fsp4HI GCNGC 2 cut(s) 261, 1194
FspBI CTAG 2 cut(s) 125, 1464
GlaI GCGC 1 cut(s) 1022
GluI GCNGC 2 cut(s) 261, 1194
GsuI CTGGAG 2 cut(s) 82, 1100
HaeII RGCGCY 1 cut(s) 1024
HaeIII GGCC 2 cut(s) 244, 718
HapII CCGG 2 cut(s) 1062, 1326
HhaI GCGC 1 cut(s) 1023
Hin1II CATG 3 cut(s) 709, 1169, 1222
Hin6I GCGC 1 cut(s) 1021
HinP1I GCGC 1 cut(s) 1021
HindIII AAGCTT 2 cut(s) 640, 791
HinfI GANTC 8 cut(s) 19, 64, 76, 96, 281, 556, 922, 1341
HpaII CCGG 2 cut(s) 1062, 1326
HphI GGTGA 3 cut(s) 71, 176, 455
Hpy188I TCNGA 5 cut(s) 624, 667, 853, 921, 1346
Hpy188III TCNNGA 6 cut(s) 28, 910, 1013, 1166, 1279, 1505
HpyAV CCTTC 5 cut(s) 371, 398, 496, 619, 1234
HpyCH4III ACNGT 5 cut(s) 523, 685, 805, 985, 1501
HpyCH4IV ACGT 6 cut(s) 350, 360, 423, 529, 1005, 1471
HpyCH4V TGCA 5 cut(s) 254, 527, 956, 1274, 1520
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 135, 260, 479, 590, 715, 1365
HpyF3I CTNAG 3 cut(s) 134, 230, 594
HpySE526I ACGT 6 cut(s) 350, 360, 423, 529, 1005, 1471
Hsp92II CATG 3 cut(s) 709, 1169, 1222
HspAI GCGC 1 cut(s) 1021
Kzo9I GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
LmnI GCTCC 5 cut(s) 772, 1081, 1183, 1400, 1484
Lsp1109I GCAGC 2 cut(s) 272, 1180
LweI GCATC 1 cut(s) 1261
MaeI CTAG 2 cut(s) 125, 1464
MaeII ACGT 6 cut(s) 350, 360, 423, 529, 1005, 1471
MaeIII GTNAC 5 cut(s) 356, 412, 466, 799, 1066
MalI GATC 6 cut(s) 85, 429, 447, 507, 936, 1217
MbiI CCGCTC 1 cut(s) 1403
MboI GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 2 cut(s) 1299, 1370
MlsI TGGCCA 1 cut(s) 244
MluCI AATT 9 cut(s) 156, 169, 321, 344, 609, 651, 726, 836, 947
MluNI TGGCCA 1 cut(s) 244
MlyI GAGTC 4 cut(s) 70, 73, 105, 1350
MmeI TCCRAC 3 cut(s) 1179, 1324, 1333
Mox20I TGGCCA 1 cut(s) 244
MroXI GAANNNNTTC 2 cut(s) 325, 381
MscI TGGCCA 1 cut(s) 244
MseI TTAA 5 cut(s) 644, 761, 777, 1046, 1152
MslI CAYNNNNRTG 1 cut(s) 1164
Msp20I TGGCCA 1 cut(s) 244
MspCI CTTAAG 3 cut(s) 776, 1045, 1151
MspI CCGG 2 cut(s) 1062, 1326
MspR9I CCNGG 2 cut(s) 318, 657
MvaI CCWGG 2 cut(s) 318, 657
MwoI GCNNNNNNNGC 7 cut(s) 53, 135, 260, 479, 590, 715, 1365
NdeII GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
NlaIII CATG 3 cut(s) 709, 1169, 1222
NlaIV GGNNCC 2 cut(s) 1324, 1480
NmuCI GTSAC 2 cut(s) 412, 466
NspI RCATGY 1 cut(s) 709
NspV TTCGAA 1 cut(s) 945
PagI TCATGA 1 cut(s) 1165
PdmI GAANNNNTTC 2 cut(s) 325, 381
PfeI GAWTC 4 cut(s) 19, 281, 556, 922
PfoI TCCNGGA 1 cut(s) 655
PinAI ACCGGT 1 cut(s) 1325
PkrI GCNGC 2 cut(s) 262, 1195
Ple19I CGATCG 1 cut(s) 86
PleI GAGTC 4 cut(s) 70, 72, 104, 1349
PmaCI CACGTG 1 cut(s) 530
PmlI CACGTG 1 cut(s) 530
PpsI GAGTC 4 cut(s) 70, 72, 104, 1349
Ppu21I YACGTR 1 cut(s) 530
PshAI GACNNNNGTC 1 cut(s) 1008
Psp124BI GAGCTC 1 cut(s) 1370
Psp6I CCWGG 2 cut(s) 316, 655
PspCI CACGTG 1 cut(s) 530
PspGI CCWGG 2 cut(s) 316, 655
PspN4I GGNNCC 2 cut(s) 1324, 1480
PspPI GGNCC 1 cut(s) 1323
PsuI RGATCY 1 cut(s) 505
PvuI CGATCG 1 cut(s) 86
RsaI GTAC 1 cut(s) 1158
RsaNI GTAC 1 cut(s) 1157
RseI CAYNNNNRTG 1 cut(s) 1164
SacI GAGCTC 1 cut(s) 1370
SaqAI TTAA 5 cut(s) 644, 761, 777, 1046, 1152
SatI GCNGC 2 cut(s) 261, 1194
Sau3AI GATC 6 cut(s) 83, 427, 445, 505, 934, 1215
Sau96I GGNCC 1 cut(s) 1323
SchI GAGTC 4 cut(s) 70, 73, 105, 1350
ScrFI CCNGG 2 cut(s) 318, 657
SduI GDGCHC 2 cut(s) 1299, 1370
SfaNI GCATC 1 cut(s) 1261
SfcI CTRYAG 1 cut(s) 984
SfuI TTCGAA 1 cut(s) 945
SinI GGWCC 1 cut(s) 1323
SmiMI CAYNNNNRTG 1 cut(s) 1164
SmlI CTYRAG 3 cut(s) 776, 1045, 1151
SmoI CTYRAG 3 cut(s) 776, 1045, 1151
Sse9I AATT 9 cut(s) 156, 169, 321, 344, 609, 651, 726, 836, 947
SsiI CCGC 4 cut(s) 548, 1359, 1392, 1403
SspI AATATT 1 cut(s) 454
SspMI CTAG 2 cut(s) 125, 1464
SstI GAGCTC 1 cut(s) 1370
StyD4I CCNGG 2 cut(s) 316, 655
StyI CCWWGG 2 cut(s) 102, 1024
TaaI ACNGT 5 cut(s) 523, 685, 805, 985, 1501
TaiI ACGT 6 cut(s) 353, 363, 426, 532, 1008, 1474
TaqI TCGA 3 cut(s) 433, 909, 945
TasI AATT 9 cut(s) 156, 169, 321, 344, 609, 651, 726, 836, 947
TfiI GAWTC 4 cut(s) 19, 281, 556, 922
Tru1I TTAA 5 cut(s) 644, 761, 777, 1046, 1152
Tru9I TTAA 5 cut(s) 644, 761, 777, 1046, 1152
TscAI CASTG 2 cut(s) 719, 988
TseFI GTSAC 2 cut(s) 412, 466
TseI GCWGC 2 cut(s) 260, 1193
Tsp45I GTSAC 2 cut(s) 412, 466
TspDTI ATGAA 1 cut(s) 131
TspRI CASTG 2 cut(s) 719, 988
Vha464I CTTAAG 3 cut(s) 776, 1045, 1151
VpaK11BI GGWCC 1 cut(s) 1323
XapI RAATTY 1 cut(s) 156
XceI RCATGY 1 cut(s) 709
XmnI GAANNNNTTC 2 cut(s) 325, 381
XspI CTAG 2 cut(s) 125, 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.