pycom02g16290

Synaptotagmin-like mitochondrial-lipid-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012269
Physical Location & Seq
Reverse (-)
13409617 .. 13412303
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g16290.1

Sequence Viewer

Length: 939 bp
ATGATTGATGATACCGTGGAATCCATTGTCACAGACATGCTCCAGTGGCCACACAGGATTGTTGTTCCAATAGGTGGTGTGCCTGTTGATACAAGTGAACTGGAGCTTAAACCTCAAGGAAAGCTCACCTTGACTGTTGCGAAAGCAAATGATCTGAAGAACATGGAAATGATTGGAAAATCGGACCCTTACGTTGTTGTCTACATTCGTCCACTGTTCAAGGTTAAGACCAAGGTTATTGATAACAATCTGAATCCTGTTTGGGACCAAACTTTCGAGTTGATTGCAGAAGACAAGGAGACTCAATCACTTATTCTTGAGGTTTTTGATGAGGACATTGGACAAGACAAGCGATTGGGAGTAACAAAATTACCTTTGATTGAGCTGGAAGCAGAAACTCCTAAAGAGATTGAACTGAGACTGCAGCCATCACTTAACATGCTAAAAATAAAGGAAAAGAAGGATAGAGGAACCATTACAATCAAGGTCTTTTACCATGAATTTAACAAGGAAGAGCAGTTGATTGCTTTAGAAGAAGAGAAGAGGATCCTAGAAGAAAGAAAGAAACTGAAAGAAGCAGGAGTTATCGGCAGCACAATGGATGCACTTGATGGAGCAGCTTCAGTGGTTGGATCCGGTGTTGGAATGGTGGGAAGTGGTGTGACCGCTGGAGCCGGGCTTGTGGGAAGTGGAGTCACTGCTGGAGCCGGGCTTGTGGGAAGTGGTGTCACTGCTGGAGCTGGGCTTGTGGGAAGTGGCGTTAATTCTGGAGTTGGAATGGTTGGGAGTGGGCTGGGGGCTGTTGGCAGTGGCCTGAGCAAAGCTGGGAAGTTCATGGGTAGGACTATCACCGGGCAATCTAGCCATTCCAGGAGAAGAAGTGGCTCCACAACTCCAGTGAACAGTGTCCAAGAAAATGGCGGTGCAAAGCCGCGGTAA

Protein Analysis

313

Amino Acids

33.05

Weight (kDa)

5.82

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 40 - 136 3e-23 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61050 AT3G61050
fragaria_vesca FvH4_7g12480 FvH4_7g12480
malus_domestica MD02G1198800.v1.1
prunus_persica Prupe.2G149400_v2.0.a1 Prupe.2G149400_v2.0.a1
pyrus_communis pycom02g16290
rosa_chinensis RchiOBHm_Chr1g0351321
rosa_laevigata RLG00000028415
rosa_multiflora Rmu_sc0004933.1_g000008 Rmu_sc0013758.1_g000001
rosa_roxburghii Rroxscaffold_4G00303880
rosa_rugosa Rorug01G0217600
rosa_samantha Rh1AG232000 Rh1BG202400 Rh1CG216800 Rh1DG229600
rosa_wichuraiana Rw1G020210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 74
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 934
AciI CCGC 4 cut(s) 666, 921, 932, 934
AclWI GGATC 4 cut(s) 541, 554, 627, 640
AcoI YGGCCR 1 cut(s) 47
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 2 cut(s) 176, 606
AfiI CCNNNNNNNGG 1 cut(s) 74
AgsI TTSAA 2 cut(s) 220, 413
AjnI CCWGG 1 cut(s) 869
AluBI AGCT 6 cut(s) 106, 124, 385, 620, 740, 824
AluI AGCT 6 cut(s) 106, 124, 385, 620, 740, 824
Alw26I GTCTC 2 cut(s) 293, 412
AlwI GGATC 4 cut(s) 541, 554, 627, 640
AoxI GGCC 2 cut(s) 47, 811
ApeKI GCWGC 3 cut(s) 424, 591, 617
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspS9I GGNCC 2 cut(s) 184, 265
AsuC2I CCSGG 3 cut(s) 676, 709, 853
AsuHPI GGTGA 2 cut(s) 118, 841
AvaII GGWCC 2 cut(s) 184, 265
BalI TGGCCA 1 cut(s) 49
BamHI GGATCC 2 cut(s) 546, 632
BbsI GAAGAC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 436, 603, 629
BccI CCATC 2 cut(s) 436, 605
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 1 cut(s) 871
BcnI CCSGG 3 cut(s) 676, 709, 853
BcoDI GTCTC 2 cut(s) 293, 412
BfaI CTAG 2 cut(s) 551, 861
BfmI CTRYAG 1 cut(s) 422
BisI GCNGC 4 cut(s) 425, 592, 618, 932
BlsI GCNGC 4 cut(s) 426, 593, 619, 933
Bme1390I CCNGG 4 cut(s) 676, 709, 853, 871
Bme18I GGWCC 2 cut(s) 184, 265
BmgT120I GGNCC 2 cut(s) 184, 265
BmiI GGNNCC 8 cut(s) 186, 266, 472, 548, 634, 673, 706, 886
BmrFI CCNGG 4 cut(s) 676, 709, 853, 871
BmsI GCATC 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 297
BpmI CTGGAG 7 cut(s) 26, 122, 690, 723, 756, 789, 879
Bpu10I CCTNAGC 1 cut(s) 815
BpuEI CTTGAG 2 cut(s) 99, 338
BpuMI CCSGG 3 cut(s) 676, 709, 853
BsaBI GATNNNNATC 1 cut(s) 246
BsaJI CCNNGG 3 cut(s) 15, 231, 932
BsaWI WCCGGW 1 cut(s) 635
BsaXI ACNNNNNCTCC 2 cut(s) 865, 895
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 43, 105, 896
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 1 cut(s) 871
BseDI CCNNGG 3 cut(s) 15, 231, 932
BseGI GGATG 1 cut(s) 607
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMII CTCAG 2 cut(s) 407, 806
BseNI ACTGG 3 cut(s) 43, 105, 896
BseXI GCAGC 3 cut(s) 436, 603, 629
BseYI CCCAGC 3 cut(s) 740, 793, 824
Bsh1236I CGCG 1 cut(s) 934
BshFI GGCC 2 cut(s) 49, 813
BsiSI CCGG 4 cut(s) 636, 675, 708, 852
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 2 cut(s) 293, 412
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 2 cut(s) 49, 813
Bsp143I GATC 3 cut(s) 151, 546, 632
BspACI CCGC 4 cut(s) 666, 921, 932, 934
BspANI GGCC 2 cut(s) 49, 813
BspCNI CTCAG 2 cut(s) 408, 807
BspFNI CGCG 1 cut(s) 934
BspLI GGNNCC 8 cut(s) 186, 266, 472, 548, 634, 673, 706, 886
BspMAI CTGCAG 1 cut(s) 426
BspPI GGATC 4 cut(s) 541, 554, 627, 640
BspQI GCTCTTC 1 cut(s) 507
BsrI ACTGG 3 cut(s) 43, 105, 896
BssECI CCNNGG 3 cut(s) 15, 231, 932
BssMI GATC 3 cut(s) 151, 546, 632
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 871
Bst4CI ACNGT 4 cut(s) 16, 136, 216, 905
Bst6I CTCTTC 3 cut(s) 507, 531, 536
BstDEI CTNAG 2 cut(s) 416, 815
BstDSI CCRYGG 2 cut(s) 15, 932
BstF5I GGATG 1 cut(s) 607
BstFNI CGCG 1 cut(s) 934
BstKTI GATC 3 cut(s) 154, 549, 635
BstMAI GTCTC 2 cut(s) 293, 412
BstMBI GATC 3 cut(s) 151, 546, 632
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 1 cut(s) 871
BstNSI RCATGY 2 cut(s) 40, 442
BstSCI CCNGG 4 cut(s) 674, 707, 851, 869
BstSFI CTRYAG 1 cut(s) 422
BstUI CGCG 1 cut(s) 934
BstV1I GCAGC 3 cut(s) 436, 603, 629
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 2 cut(s) 546, 632
BstXI CCANNNNNNTGG 1 cut(s) 917
BstYI RGATCY 2 cut(s) 546, 632
BsuRI GGCC 2 cut(s) 49, 813
BtgI CCRYGG 2 cut(s) 15, 932
BtsCI GGATG 1 cut(s) 607
BtsI GCAGTG 3 cut(s) 696, 729, 814
BtsIMutI CAGTG 8 cut(s) 50, 212, 630, 696, 729, 814, 903, 910
Cfr13I GGNCC 2 cut(s) 184, 265
Cfr42I CCGCGG 1 cut(s) 935
CviAII CATG 5 cut(s) 37, 163, 439, 497, 835
DdeI CTNAG 2 cut(s) 416, 815
DpnI GATC 3 cut(s) 153, 548, 634
DpnII GATC 3 cut(s) 151, 546, 632
EaeI YGGCCR 1 cut(s) 47
Eam1104I CTCTTC 3 cut(s) 507, 531, 536
EarI CTCTTC 3 cut(s) 507, 531, 536
Eco130I CCWWGG 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 184, 265
Eco57I CTGAAG 2 cut(s) 176, 606
EcoRII CCWGG 1 cut(s) 869
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 5 cut(s) 40, 166, 442, 500, 838
FaiI YATR 5 cut(s) 38, 164, 440, 498, 836
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FaqI GGGAC 1 cut(s) 278
FatI CATG 5 cut(s) 36, 162, 438, 496, 834
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 4 cut(s) 425, 592, 618, 932
FokI GGATG 1 cut(s) 614
Fsp4HI GCNGC 4 cut(s) 425, 592, 618, 932
FspBI CTAG 2 cut(s) 551, 861
GluI GCNGC 4 cut(s) 425, 592, 618, 932
GsaI CCCAGC 3 cut(s) 744, 797, 828
GsuI CTGGAG 7 cut(s) 26, 122, 690, 723, 756, 789, 879
HaeIII GGCC 2 cut(s) 49, 813
HapII CCGG 4 cut(s) 636, 675, 708, 852
Hin1II CATG 5 cut(s) 40, 166, 442, 500, 838
HinfI GANTC 4 cut(s) 20, 253, 301, 693
HpaII CCGG 4 cut(s) 636, 675, 708, 852
HphI GGTGA 2 cut(s) 118, 841
Hpy166II GTNNAC 4 cut(s) 98, 202, 212, 901
Hpy188I TCNGA 3 cut(s) 156, 184, 252
Hpy188III TCNNGA 2 cut(s) 317, 768
Hpy8I GTNNAC 4 cut(s) 98, 202, 212, 901
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 4 cut(s) 16, 136, 216, 905
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 4 cut(s) 287, 424, 605, 926
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 2 cut(s) 416, 815
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 5 cut(s) 40, 166, 442, 500, 838
KspI CCGCGG 1 cut(s) 935
Kzo9I GATC 3 cut(s) 151, 546, 632
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 7 cut(s) 45, 103, 614, 671, 704, 737, 890
Lsp1109I GCAGC 3 cut(s) 436, 603, 629
LweI GCATC 1 cut(s) 592
MaeI CTAG 2 cut(s) 551, 861
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 5 cut(s) 28, 361, 661, 694, 727
MalI GATC 3 cut(s) 153, 548, 634
MboI GATC 3 cut(s) 151, 546, 632
MboII GAAGA 8 cut(s) 169, 302, 524, 545, 548, 553, 566, 888
MflI RGATCY 2 cut(s) 546, 632
MlsI TGGCCA 1 cut(s) 49
MluCI AATT 3 cut(s) 368, 500, 763
MluNI TGGCCA 1 cut(s) 49
MlyI GAGTC 2 cut(s) 295, 702
MmeI TCCRAC 3 cut(s) 610, 622, 754
MnlI CCTC 5 cut(s) 123, 313, 325, 461, 537
Mox20I TGGCCA 1 cut(s) 49
MscI TGGCCA 1 cut(s) 49
MseI TTAA 5 cut(s) 108, 225, 435, 504, 762
MslI CAYNNNNRTG 2 cut(s) 35, 167
Msp20I TGGCCA 1 cut(s) 49
MspA1I CMGCKG 2 cut(s) 668, 934
MspI CCGG 4 cut(s) 636, 675, 708, 852
MspR9I CCNGG 4 cut(s) 676, 709, 853, 871
MvaI CCWGG 1 cut(s) 871
MvnI CGCG 1 cut(s) 934
MwoI GCNNNNNNNGC 1 cut(s) 46
NciI CCSGG 3 cut(s) 676, 709, 853
NdeII GATC 3 cut(s) 151, 546, 632
NlaIII CATG 5 cut(s) 40, 166, 442, 500, 838
NlaIV GGNNCC 8 cut(s) 186, 266, 472, 548, 634, 673, 706, 886
NmuCI GTSAC 4 cut(s) 28, 661, 694, 727
NspI RCATGY 2 cut(s) 40, 442
PciSI GCTCTTC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 20, 253
PflMI CCANNNNNTGG 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 869
PkrI GCNGC 4 cut(s) 426, 593, 619, 933
PleI GAGTC 2 cut(s) 295, 701
PpsI GAGTC 2 cut(s) 295, 701
Psp6I CCWGG 1 cut(s) 869
PspFI CCCAGC 3 cut(s) 740, 793, 824
PspGI CCWGG 1 cut(s) 869
PspN4I GGNNCC 8 cut(s) 186, 266, 472, 548, 634, 673, 706, 886
PspPI GGNCC 2 cut(s) 184, 265
PstI CTGCAG 1 cut(s) 426
PsuI RGATCY 2 cut(s) 546, 632
RseI CAYNNNNRTG 2 cut(s) 35, 167
SacII CCGCGG 1 cut(s) 935
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 5 cut(s) 108, 225, 435, 504, 762
SatI GCNGC 4 cut(s) 425, 592, 618, 932
Sau3AI GATC 3 cut(s) 151, 546, 632
Sau96I GGNCC 2 cut(s) 184, 265
SchI GAGTC 2 cut(s) 295, 702
ScrFI CCNGG 4 cut(s) 676, 709, 853, 871
SfaNI GCATC 1 cut(s) 592
SfcI CTRYAG 1 cut(s) 422
Sfr303I CCGCGG 1 cut(s) 935
SgrBI CCGCGG 1 cut(s) 935
SinI GGWCC 2 cut(s) 184, 265
SmiMI CAYNNNNRTG 2 cut(s) 35, 167
SmlI CTYRAG 2 cut(s) 114, 317
SmoI CTYRAG 2 cut(s) 114, 317
Sse9I AATT 3 cut(s) 368, 500, 763
SsiI CCGC 4 cut(s) 666, 921, 932, 934
SspMI CTAG 2 cut(s) 551, 861
StyD4I CCNGG 4 cut(s) 674, 707, 851, 869
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 4 cut(s) 16, 136, 216, 905
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 276
TasI AATT 3 cut(s) 368, 500, 763
TauI GCSGC 1 cut(s) 934
TfiI GAWTC 2 cut(s) 20, 253
Tru1I TTAA 5 cut(s) 108, 225, 435, 504, 762
Tru9I TTAA 5 cut(s) 108, 225, 435, 504, 762
TscAI CASTG 8 cut(s) 50, 219, 630, 703, 736, 814, 903, 910
TseFI GTSAC 4 cut(s) 28, 661, 694, 727
TseI GCWGC 3 cut(s) 424, 591, 617
Tsp45I GTSAC 4 cut(s) 28, 661, 694, 727
TspDTI ATGAA 2 cut(s) 513, 823
TspRI CASTG 8 cut(s) 50, 219, 630, 703, 736, 814, 903, 910
Van91I CCANNNNNTGG 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 184, 265
XapI RAATTY 1 cut(s) 500
XceI RCATGY 2 cut(s) 40, 442
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 2 cut(s) 551, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.