Rroxscaffold_4G00303880

Synaptotagmin-like mitochondrial-lipid-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012269
Physical Location & Seq
Reverse (-)
24370381 .. 24377732
7352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303880.1

Sequence Viewer

Length: 1449 bp
ATGGGGTTGATTTCGGGGATTCTGATGGGGATGGTGTTCGGAATTGCGTTGATGGCCGGCTGGCGTCACATGATGGCGTACCGGAGTACCAAGCGAGTCGCTAAGGTAGAAATGGCAGTTGATATAAAACTCCTTGGGTCACTAAACAGAGACGATTTGAAGAAAATTTGTGGGGACAACTTTCCTGAGTGGATTTCTTTCCCCGTATTTGAACAGGTGAAATGGTTGAATAAGCAGCTGAGCAAACTATGGCCATTTGTTGCAGATGCAGCAGAGCTGGTGATAAAAGAGTCTGTTGAACCACTATTGGAAGAATACAGACCTCCGGGAATCTCATCATTGAAGTTTAGCAAACTGTCTCTTGGTACTGTGGCACCCAAAATTGAAGGTATACGTGTTCAGAGTCTTAAGAAAGGTCAAATCACGATGGACATTGATTTTCGTTGGGGTGGTGATCCAAACATTGTATTAGGTGTTGAAGCACTTGTTGCTTCAATTCCCATTCAGTTGAAGGATCTTCAAGTTTTTACTGTTGTTCGCGTAATATTCCAACTTTCCGAAGAGATCCCCTGTATTTCTGCGGTTGTTGTTGCCCTACTTGCAGAGCCAAAGCCTAGAATTGATTATACTCTGAAGGCTGTTGGTGGAAGCTTAACAGCCCTCCCTGGAATATCGGATATGATTGATGATACAGTCGAGACGATTGTCACAGACATGCTCCAGTGGCCACATAGGATAGTTGTTCCAATTGGTGGGTTGCATGTTGATACAAGTGACTTGGAGCTTAAACCTCAGGGAAAGATCACCTTAACGGTTGTCAGAGCAAATGATTTAAAGAACATGGAAATGATTGGAAAATCAGACCCTTATGTTGTTGTTTACATTCGTCCAATTTTCAAGGTTAAGACCAAGGTTATTGACAACAATCTGAATCCTGTTTGGGACCAAACATTCGAGTTGATTGCAGAAGACAAGGAGACACAATCACTTGTTTTTGAGATTTTCGATGAGGACATTGGGCAAGACAAGCGATTGGGAGTAACAAAATTACCTTTGATCGATCTGGAAGCAGAAATTCCTAAAGAGATGGAACTGAGATTGCAGCCATCTCTTAACATGCTAAAAATAAAAGAAAAGAAGGACAGAGGAACACTTACGATTAGGATCTTGTACCATGAATTCAACAAGGAAGAGCAGTTGGCTGCTTTAGAGGAAGAGAAGAGGATCTTTGAAGAAAGAAAGAAACTGAAAGCTGAAGGAGTTATAGGGAGCACAATGGATGCAATTGGGTCTGGTGCTGGAATGGTGGGTTCTGGTATCGGTCGCTGGAGTTGGGCTTGTGGGAGTGGTGTTGGCTTGTGGGAGTTGGACTTCTGGGAACTGGTGTTGGTGCTGGAGTTGGACTTCTGTGGGAAGTGGTGTCAGTTCTGGGGCTGGAATTGGTGGTAG

Protein Analysis

482

Amino Acids

54.31

Weight (kDa)

5.27

Isoelectric Point (pI)

29.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMP_LBD PF17047 71 - 250 2.2e-17 Synaptotagmin-like mitochondrial-lipid-binding domain
C2 PF00168 266 - 358 6.6e-23 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61050 AT3G61050
fragaria_vesca FvH4_7g12480 FvH4_7g12480
malus_domestica MD02G1198800.v1.1
prunus_persica Prupe.2G149400_v2.0.a1 Prupe.2G149400_v2.0.a1
pyrus_communis pycom02g16290
rosa_chinensis RchiOBHm_Chr1g0351321
rosa_laevigata RLG00000028415
rosa_multiflora Rmu_sc0004933.1_g000008 Rmu_sc0013758.1_g000001
rosa_roxburghii Rroxscaffold_4G00303880
rosa_rugosa Rorug01G0217600
rosa_samantha Rh1AG232000 Rh1BG202400 Rh1CG216800 Rh1DG229600
rosa_wichuraiana Rw1G020210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccB7I CCANNNNNTGG 1 cut(s) 752
AccI GTMKAC 1 cut(s) 391
AccII CGCG 1 cut(s) 540
AciI CCGC 1 cut(s) 581
AclWI GGATC 5 cut(s) 449, 522, 559, 1172, 1232
AcoI YGGCCR 3 cut(s) 54, 251, 725
AcsI RAATTY 3 cut(s) 165, 1074, 1178
AcuI CTGAAG 2 cut(s) 653, 1275
AcyI GRCGYC 1 cut(s) 64
AfaI GTAC 4 cut(s) 80, 88, 367, 1172
AfiI CCNNNNNNNGG 1 cut(s) 752
AflII CTTAAG 1 cut(s) 407
AflIII ACRYGT 1 cut(s) 394
AjnI CCWGG 1 cut(s) 664
AluBI AGCT 5 cut(s) 238, 277, 651, 784, 1253
AluI AGCT 5 cut(s) 238, 277, 651, 784, 1253
Alw21I GWGCWC 1 cut(s) 1274
Alw26I GTCTC 4 cut(s) 144, 363, 692, 971
AlwI GGATC 5 cut(s) 449, 522, 559, 1172, 1232
AoxI GGCC 3 cut(s) 54, 251, 725
ApeKI GCWGC 4 cut(s) 235, 269, 1102, 1202
ApoI RAATTY 3 cut(s) 165, 1074, 1178
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
AspS9I GGNCC 1 cut(s) 943
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 4 cut(s) 229, 292, 464, 796
AvaII GGWCC 1 cut(s) 943
AxyI CCTNAGG 1 cut(s) 792
BaeI ACNNNNGTAYC 2 cut(s) 70, 103
BalI TGGCCA 2 cut(s) 253, 727
BanI GGYRCC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 975
Bbv12I GWGCWC 1 cut(s) 1274
BbvI GCAGC 4 cut(s) 247, 281, 1114, 1189
BccI CCATC 7 cut(s) 19, 25, 46, 67, 421, 1081, 1114
BcgI CGANNNNNNTGC 2 cut(s) 944, 978
BciT130I CCWGG 1 cut(s) 666
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 4 cut(s) 144, 363, 692, 971
BfaI CTAG 1 cut(s) 615
BfrI CTTAAG 1 cut(s) 407
BisI GCNGC 4 cut(s) 236, 270, 1103, 1203
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 4 cut(s) 237, 271, 1104, 1204
Bme1390I CCNGG 2 cut(s) 327, 666
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 2 cut(s) 375, 944
BmrFI CCNGG 2 cut(s) 327, 666
BmsI GCATC 2 cut(s) 256, 1270
BoxI GACNNNNGTC 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 975
BpmI CTGGAG 3 cut(s) 704, 1348, 1415
Bpu10I CCTNAGC 1 cut(s) 102
Bpu1102I GCTNAGC 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 327
Bsa29I ATCGAT 1 cut(s) 1059
BsaAI YACGTR 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 1163
BsaHI GRCGYC 1 cut(s) 64
BsaJI CCNNGG 3 cut(s) 133, 664, 909
BsaWI WCCGGW 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 752
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 2 cut(s) 721, 1386
Bse21I CCTNAGG 1 cut(s) 792
Bse8I GATNNNNATC 1 cut(s) 1163
BseBI CCWGG 1 cut(s) 666
BseCI ATCGAT 1 cut(s) 1059
BseDI CCNNGG 3 cut(s) 133, 664, 909
BseGI GGATG 2 cut(s) 36, 1285
BseJI GATNNNNATC 1 cut(s) 1163
BseLI CCNNNNNNNGG 1 cut(s) 752
BseMII CTCAG 4 cut(s) 177, 230, 806, 1085
BseNI ACTGG 2 cut(s) 721, 1386
BseXI GCAGC 4 cut(s) 247, 281, 1114, 1189
Bsh1236I CGCG 1 cut(s) 540
Bsh1285I CGRYCG 1 cut(s) 1324
BshFI GGCC 3 cut(s) 56, 253, 727
BshNI GGYRCC 1 cut(s) 373
BshVI ATCGAT 1 cut(s) 1059
BsiEI CGRYCG 1 cut(s) 1324
BsiHKAI GWGCWC 1 cut(s) 1274
BsiSI CCGG 3 cut(s) 57, 82, 326
BslFI GGGAC 2 cut(s) 188, 956
BslI CCNNNNNNNGG 1 cut(s) 752
BsmAI GTCTC 4 cut(s) 144, 363, 692, 971
BsmBI CGTCTC 2 cut(s) 144, 692
BsmFI GGGAC 2 cut(s) 188, 956
BsnI GGCC 3 cut(s) 56, 253, 727
Bsp1286I GDGCHC 1 cut(s) 1274
Bsp143I GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
Bsp1720I GCTNAGC 1 cut(s) 239
BspACI CCGC 1 cut(s) 581
BspANI GGCC 3 cut(s) 56, 253, 727
BspCNI CTCAG 4 cut(s) 178, 231, 805, 1086
BspDI ATCGAT 1 cut(s) 1059
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 2 cut(s) 375, 944
BspPI GGATC 5 cut(s) 449, 522, 559, 1172, 1232
BspQI GCTCTTC 1 cut(s) 1185
BspT107I GGYRCC 1 cut(s) 373
BspTI CTTAAG 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 2 cut(s) 721, 1386
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 133, 664, 909
BssMI GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
BssNAI GTATAC 1 cut(s) 392
BssNI GRCGYC 1 cut(s) 64
BssT1I CCWWGG 2 cut(s) 133, 909
Bst1107I GTATAC 1 cut(s) 392
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 5 cut(s) 357, 370, 532, 694, 814
Bst6I CTCTTC 4 cut(s) 555, 1185, 1209, 1214
BstACI GRCGYC 1 cut(s) 64
BstAFI CTTAAG 1 cut(s) 407
BstAPI GCANNNNNTGC 1 cut(s) 488
BstBAI YACGTR 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 58, 62
BstDEI CTNAG 5 cut(s) 102, 186, 239, 792, 1094
BstF5I GGATG 2 cut(s) 36, 1285
BstFNI CGCG 1 cut(s) 540
BstKTI GATC 8 cut(s) 457, 517, 567, 804, 1059, 1063, 1167, 1227
BstMAI GTCTC 4 cut(s) 144, 363, 692, 971
BstMBI GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
BstMCI CGRYCG 1 cut(s) 1324
BstMWI GCNNNNNNNGC 6 cut(s) 53, 269, 488, 599, 724, 1027
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 3 cut(s) 718, 764, 1120
BstPAI GACNNNNGTC 1 cut(s) 704
BstSCI CCNGG 2 cut(s) 325, 664
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 4 cut(s) 247, 281, 1114, 1189
BstV2I GAAGAC 1 cut(s) 975
BstX2I RGATCY 4 cut(s) 514, 564, 1164, 1224
BstYI RGATCY 4 cut(s) 514, 564, 1164, 1224
BstZ17I GTATAC 1 cut(s) 392
Bsu15I ATCGAT 1 cut(s) 1059
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 3 cut(s) 56, 253, 727
BsuTUI ATCGAT 1 cut(s) 1059
BtsCI GGATG 2 cut(s) 36, 1285
BtsIMutI CAGTG 1 cut(s) 728
Cac8I GCNNGC 2 cut(s) 58, 62
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 943
ClaI ATCGAT 1 cut(s) 1059
CseI GACGC 1 cut(s) 53
Csp6I GTAC 4 cut(s) 79, 87, 366, 1171
CviAII CATG 6 cut(s) 70, 715, 761, 841, 1117, 1175
CviQI GTAC 4 cut(s) 79, 87, 366, 1171
DdeI CTNAG 5 cut(s) 102, 186, 239, 792, 1094
DpnI GATC 8 cut(s) 456, 516, 566, 803, 1058, 1062, 1166, 1226
DpnII GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
DraI TTTAAA 1 cut(s) 834
EaeI YGGCCR 3 cut(s) 54, 251, 725
Eam1104I CTCTTC 4 cut(s) 555, 1185, 1209, 1214
EarI CTCTTC 4 cut(s) 555, 1185, 1209, 1214
Eco130I CCWWGG 2 cut(s) 133, 909
Eco47I GGWCC 1 cut(s) 943
Eco57I CTGAAG 2 cut(s) 653, 1275
Eco81I CCTNAGG 1 cut(s) 792
EcoRI GAATTC 1 cut(s) 1178
EcoRII CCWGG 1 cut(s) 664
EcoT14I CCWWGG 2 cut(s) 133, 909
ErhI CCWWGG 2 cut(s) 133, 909
Esp3I CGTCTC 2 cut(s) 144, 692
FaeI CATG 6 cut(s) 73, 718, 764, 844, 1120, 1178
FalI AAGNNNNNCTT 4 cut(s) 791, 823, 1211, 1243
FaqI GGGAC 2 cut(s) 188, 956
FatI CATG 6 cut(s) 69, 714, 760, 840, 1116, 1174
FblI GTMKAC 1 cut(s) 391
Fnu4HI GCNGC 4 cut(s) 236, 270, 1103, 1203
FokI GGATG 2 cut(s) 43, 1292
Fsp4HI GCNGC 4 cut(s) 236, 270, 1103, 1203
FspBI CTAG 1 cut(s) 615
GluI GCNGC 4 cut(s) 236, 270, 1103, 1203
GsuI CTGGAG 3 cut(s) 704, 1348, 1415
HaeIII GGCC 3 cut(s) 56, 253, 727
HapII CCGG 3 cut(s) 57, 82, 326
HgaI GACGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 64
Hin1II CATG 6 cut(s) 73, 718, 764, 844, 1120, 1178
HindIII AAGCTT 1 cut(s) 649
HinfI GANTC 6 cut(s) 19, 96, 290, 330, 403, 931
HpaII CCGG 3 cut(s) 57, 82, 326
HphI GGTGA 4 cut(s) 229, 292, 464, 796
Hpy166II GTNNAC 2 cut(s) 392, 880
Hpy188I TCNGA 9 cut(s) 24, 41, 402, 559, 633, 676, 821, 862, 930
Hpy188III TCNNGA 4 cut(s) 185, 424, 697, 1064
Hpy8I GTNNAC 2 cut(s) 392, 880
HpyAV CCTTC 5 cut(s) 380, 505, 628, 1132, 1250
HpyCH4III ACNGT 5 cut(s) 357, 370, 532, 694, 814
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 7 cut(s) 263, 269, 602, 760, 965, 1102, 1283
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 269, 488, 599, 724, 1027
HpyF3I CTNAG 5 cut(s) 102, 186, 239, 792, 1094
HpySE526I ACGT 1 cut(s) 394
Hsp92I GRCGYC 1 cut(s) 64
Hsp92II CATG 6 cut(s) 73, 718, 764, 844, 1120, 1178
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
Kzo9I GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
LguI GCTCTTC 1 cut(s) 1185
LmnI GCTCC 3 cut(s) 723, 781, 1269
Lsp1109I GCAGC 4 cut(s) 247, 281, 1114, 1189
LweI GCATC 2 cut(s) 256, 1270
MaeI CTAG 1 cut(s) 615
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 5 cut(s) 65, 138, 706, 773, 1039
MalI GATC 8 cut(s) 456, 516, 566, 803, 1058, 1062, 1166, 1226
MboI GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
MboII GAAGA 9 cut(s) 172, 323, 509, 572, 980, 1202, 1226, 1231, 1244
MfeI CAATTG 2 cut(s) 747, 1284
MflI RGATCY 4 cut(s) 514, 564, 1164, 1224
MhlI GDGCHC 1 cut(s) 1274
MlsI TGGCCA 2 cut(s) 253, 727
MluNI TGGCCA 2 cut(s) 253, 727
MlyI GAGTC 3 cut(s) 105, 299, 412
MmeI TCCRAC 3 cut(s) 574, 1347, 1380
MnlI CCTC 7 cut(s) 333, 671, 801, 1003, 1139, 1204, 1215
Mox20I TGGCCA 2 cut(s) 253, 727
MroNI GCCGGC 1 cut(s) 56
MscI TGGCCA 2 cut(s) 253, 727
MseI TTAA 7 cut(s) 408, 653, 786, 809, 833, 903, 1113
MslI CAYNNNNRTG 2 cut(s) 713, 845
Msp20I TGGCCA 2 cut(s) 253, 727
MspA1I CMGCKG 1 cut(s) 238
MspCI CTTAAG 1 cut(s) 407
MspI CCGG 3 cut(s) 57, 82, 326
MspR9I CCNGG 2 cut(s) 327, 666
MunI CAATTG 2 cut(s) 747, 1284
MvaI CCWGG 1 cut(s) 666
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 6 cut(s) 53, 269, 488, 599, 724, 1027
NaeI GCCGGC 1 cut(s) 58
NciI CCSGG 1 cut(s) 327
NdeII GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
NgoMIV GCCGGC 1 cut(s) 56
NlaIII CATG 6 cut(s) 73, 718, 764, 844, 1120, 1178
NlaIV GGNNCC 2 cut(s) 375, 944
NmuCI GTSAC 4 cut(s) 65, 138, 706, 773
NspI RCATGY 3 cut(s) 718, 764, 1120
PciSI GCTCTTC 1 cut(s) 1185
PdiI GCCGGC 1 cut(s) 58
PfeI GAWTC 3 cut(s) 19, 330, 931
PflMI CCANNNNNTGG 1 cut(s) 752
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 4 cut(s) 237, 271, 1104, 1204
PleI GAGTC 3 cut(s) 104, 298, 411
PpsI GAGTC 3 cut(s) 104, 298, 411
Ppu21I YACGTR 1 cut(s) 395
PshAI GACNNNNGTC 1 cut(s) 704
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 2 cut(s) 375, 944
PspPI GGNCC 1 cut(s) 943
PsuI RGATCY 4 cut(s) 514, 564, 1164, 1224
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 4 cut(s) 80, 88, 367, 1172
RsaNI GTAC 4 cut(s) 79, 87, 366, 1171
RseI CAYNNNNRTG 2 cut(s) 713, 845
SapI GCTCTTC 1 cut(s) 1185
SaqAI TTAA 7 cut(s) 408, 653, 786, 809, 833, 903, 1113
SatI GCNGC 4 cut(s) 236, 270, 1103, 1203
Sau3AI GATC 8 cut(s) 454, 514, 564, 801, 1056, 1060, 1164, 1224
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 3 cut(s) 105, 299, 412
ScrFI CCNGG 2 cut(s) 327, 666
SduI GDGCHC 1 cut(s) 1274
SfaNI GCATC 2 cut(s) 256, 1270
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 2 cut(s) 713, 845
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
SsiI CCGC 1 cut(s) 581
SspI AATATT 1 cut(s) 546
SspMI CTAG 1 cut(s) 615
StyD4I CCNGG 2 cut(s) 325, 664
StyI CCWWGG 2 cut(s) 133, 909
TaaI ACNGT 5 cut(s) 357, 370, 532, 694, 814
TaiI ACGT 1 cut(s) 397
TaqI TCGA 4 cut(s) 696, 954, 1005, 1059
TaqII GACCGA 1 cut(s) 1310
TfiI GAWTC 3 cut(s) 19, 330, 931
Tru1I TTAA 7 cut(s) 408, 653, 786, 809, 833, 903, 1113
Tru9I TTAA 7 cut(s) 408, 653, 786, 809, 833, 903, 1113
TscAI CASTG 1 cut(s) 728
TseFI GTSAC 4 cut(s) 65, 138, 706, 773
TseI GCWGC 4 cut(s) 235, 269, 1102, 1202
Tsp45I GTSAC 4 cut(s) 65, 138, 706, 773
TspDTI ATGAA 1 cut(s) 1191
TspRI CASTG 1 cut(s) 728
Van91I CCANNNNNTGG 1 cut(s) 752
Vha464I CTTAAG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 3 cut(s) 165, 1074, 1178
XceI RCATGY 3 cut(s) 718, 764, 1120
XmiI GTMKAC 1 cut(s) 391
XspI CTAG 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.