AT3G62370

Ethylbenzene dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010461
Physical Location & Seq
Forward (+)
23080706 .. 23083528
2823 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62370.1

Sequence Viewer

Length: 1086 bp
ATGCTTCAGCTCGTGGTTGTAGCGTGGCTCTTGATTTTCACGGCGGAGACGGTTGTACCTCACCAGGAATCCGGCGAATGGAGTTGTGAATCGGACTCGGAGATTCAGGTCTTAGCCGATTTCAGACCGGGGATTATCACCCTCGATGGTCACAACGATGACTGGAAGGACATTGACGGCTCCGAGTTCCCGCTCCGACCTGCACTTGATCCGGACTCCGATCACGAGTATGACGCCGGGAAGATGACAGTCAAGGCACTACATGATGGTCGAGATATCTACTTTCTTCTGGAGATTGATGGAAATTACGCTTATGATAAAGGTGAAAACAACAAGTGTCCTTCAGTGGCTCTCATGTTCCAAATTGGTGATCAAGCTACTTATCACAATATGGGTGGTTGTAAAGAAGGGACAGACTCATGTACCAGCAAGGCTTGCAGAGGCTTCGAGGTTGATATTATGCATTTCTCCATTGGAAATGCTATTCCAGGACGGCTTTACGGTGGTAATCCAATAGACAACGGGGAAGGAAATGGAGGTGACAGATTTGGTCATCTGGTTGACATTTATGCATGGAATCCACATTGTCGGTACCTTGATGGTCTTGGACCCTCAGGAAATGATTCCAGTGCACAGAACGATTGGCATGGAGCGTGGTGGCACAGCAGCTTCACCACTCTATCAGGTTATGTAGAGGAAGACAGCCCTTACACGCCAGATGGCAAGAAAGGAACTTACTATTTTGAGTTTTCAAGGCCCTTGAGGACAATGGACCGTCTCCAACAGGATGTTCAGTTCACGCTTGGCTCGCCGGCCAAAATGTCCGTCGCATTCTGGTACCCAGTGGACAGCAAACCATGGCATGGATCAGGACACTACACGATCAACTGCGATTGGACACCGCTAGATATCTCCTCGGGGTCTTCTTCGGGTCTCACGGCTTCAACTGTCAAGGGCTCTAGCGATGGGGCCAGCATTACAGCCATTTTGTTGTCCATGATATCTCTTGTGTTTTCGGGTTTTATTGCATATAGGCTATTTAGTCCCAGGAACGTTCCATTCACACCCATGGAAAACAATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EB_dh

Protein Analysis

361

Amino Acids

39.85

Weight (kDa)

4.66

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EB_dh PF09459 52 - 293 7.2e-50 Ethylbenzene dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 208
Acc65I GGTACC 2 cut(s) 591, 837
AccB1I GGYRCC 2 cut(s) 591, 837
AccB7I CCANNNNNTGG 1 cut(s) 863
AccBSI CCGCTC 1 cut(s) 193
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 3 cut(s) 44, 191, 902
AclI AACGTT 1 cut(s) 1053
AclWI GGATC 2 cut(s) 203, 874
AcoI YGGCCR 1 cut(s) 813
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 4 cut(s) 57, 424, 593, 839
AfiI CCNNNNNNNGG 2 cut(s) 78, 863
AgsI TTSAA 2 cut(s) 753, 945
AjnI CCWGG 3 cut(s) 63, 487, 1046
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AloI GAACNNNNNNTCC 2 cut(s) 779, 811
AluBI AGCT 3 cut(s) 10, 377, 669
AluI AGCT 3 cut(s) 10, 377, 669
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 3 cut(s) 41, 782, 938
Alw44I GTGCAC 1 cut(s) 630
AlwI GGATC 2 cut(s) 203, 874
Ama87I CYCGRG 1 cut(s) 916
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 3 cut(s) 755, 813, 969
ApaLI GTGCAC 1 cut(s) 630
ApeKI GCWGC 1 cut(s) 666
ArsI GACNNNNNNTTYG 2 cut(s) 810, 842
Asp700I GAANNNNTTC 1 cut(s) 622
Asp718I GGTACC 2 cut(s) 591, 837
AspS9I GGNCC 4 cut(s) 608, 756, 772, 969
AsuC2I CCSGG 2 cut(s) 129, 238
AsuHPI GGTGA 6 cut(s) 53, 130, 335, 380, 551, 664
AvaI CYCGRG 1 cut(s) 916
AvaII GGWCC 2 cut(s) 608, 772
AxyI CCTNAGG 1 cut(s) 613
BaeGI GKGCMC 1 cut(s) 634
BaeI ACNNNNGTAYC 4 cut(s) 39, 72, 406, 439
BanI GGYRCC 2 cut(s) 591, 837
BanII GRGCYC 1 cut(s) 959
BauI CACGAG 2 cut(s) 11, 224
BbsI GAAGAC 2 cut(s) 705, 915
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 1 cut(s) 678
BccI CCATC 6 cut(s) 140, 260, 293, 593, 713, 959
BceAI ACGGC 4 cut(s) 57, 193, 509, 954
BcgI CGANNNNNNTGC 2 cut(s) 427, 461
BciT130I CCWGG 3 cut(s) 65, 489, 1048
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 2 cut(s) 129, 238
BcoDI GTCTC 3 cut(s) 41, 782, 938
BfaI CTAG 2 cut(s) 905, 960
BfmI CTRYAG 1 cut(s) 1082
BfuAI ACCTGC 1 cut(s) 208
BisI GCNGC 1 cut(s) 667
BlsI GCNGC 1 cut(s) 668
Bme1390I CCNGG 5 cut(s) 65, 129, 238, 489, 1048
Bme18I GGWCC 2 cut(s) 608, 772
BmeT110I CYCGRG 1 cut(s) 916
BmgT120I GGNCC 4 cut(s) 608, 756, 772, 969
BmiI GGNNCC 5 cut(s) 181, 593, 610, 839, 970
BmrFI CCNGG 5 cut(s) 65, 129, 238, 489, 1048
BmrI ACTGGG 1 cut(s) 836
BmuI ACTGGG 1 cut(s) 836
BpiI GAAGAC 2 cut(s) 705, 915
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 781
BpuMI CCSGG 2 cut(s) 129, 238
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 5 cut(s) 128, 857, 915, 1046, 1068
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 863
Bse118I RCCGGY 1 cut(s) 811
Bse1I ACTGG 3 cut(s) 167, 627, 842
Bse21I CCTNAGG 1 cut(s) 613
BseAI TCCGGA 1 cut(s) 211
BseBI CCWGG 3 cut(s) 65, 489, 1048
BseDI CCNNGG 5 cut(s) 128, 857, 915, 1046, 1068
BseGI GGATG 1 cut(s) 793
BseLI CCNNNNNNNGG 2 cut(s) 78, 863
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 3 cut(s) 167, 627, 842
BseRI GAGGAG 1 cut(s) 904
BseSI GKGCMC 1 cut(s) 634
BseXI GCAGC 1 cut(s) 678
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 3 cut(s) 757, 815, 971
BshNI GGYRCC 2 cut(s) 591, 837
BsiHKAI GWGCWC 1 cut(s) 634
BsiHKCI CYCGRG 1 cut(s) 916
BsiSI CCGG 5 cut(s) 72, 128, 212, 237, 812
BslFI GGGAC 2 cut(s) 424, 1029
BslI CCNNNNNNNGG 2 cut(s) 78, 863
BsmAI GTCTC 3 cut(s) 41, 782, 938
BsmBI CGTCTC 2 cut(s) 41, 782
BsmFI GGGAC 2 cut(s) 424, 1029
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 3 cut(s) 757, 815, 971
Bso31I GGTCTC 1 cut(s) 938
BsoBI CYCGRG 1 cut(s) 916
Bsp1286I GDGCHC 2 cut(s) 634, 959
Bsp13I TCCGGA 1 cut(s) 211
Bsp143I GATC 5 cut(s) 208, 220, 370, 866, 882
Bsp19I CCATGG 2 cut(s) 857, 1068
BspACI CCGC 3 cut(s) 44, 191, 902
BspANI GGCC 3 cut(s) 757, 815, 971
BspCNI CTCAG 1 cut(s) 626
BspEI TCCGGA 1 cut(s) 211
BspLI GGNNCC 5 cut(s) 181, 593, 610, 839, 970
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 2 cut(s) 203, 874
BspT107I GGYRCC 2 cut(s) 591, 837
BspTNI GGTCTC 1 cut(s) 938
BsrBI CCGCTC 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 811
BsrI ACTGG 3 cut(s) 167, 627, 842
BssAI RCCGGY 1 cut(s) 811
BssECI CCNNGG 5 cut(s) 128, 857, 915, 1046, 1068
BssMI GATC 5 cut(s) 208, 220, 370, 866, 882
BssNI GRCGYC 1 cut(s) 234
BssSI CACGAG 2 cut(s) 11, 224
BssT1I CCWWGG 2 cut(s) 857, 1068
Bst2BI CACGAG 2 cut(s) 11, 224
Bst2UI CCWGG 3 cut(s) 65, 489, 1048
Bst4CI ACNGT 5 cut(s) 52, 250, 503, 776, 949
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 435
BstC8I GCNNGC 4 cut(s) 436, 809, 813, 973
BstDEI CTNAG 2 cut(s) 112, 613
BstDSI CCRYGG 2 cut(s) 857, 1068
BstF5I GGATG 1 cut(s) 793
BstKTI GATC 5 cut(s) 211, 223, 373, 869, 885
BstMAI GTCTC 3 cut(s) 41, 782, 938
BstMBI GATC 5 cut(s) 208, 220, 370, 866, 882
BstMWI GCNNNNNNNGC 2 cut(s) 435, 808
BstNI CCWGG 3 cut(s) 65, 489, 1048
BstSCI CCNGG 5 cut(s) 63, 127, 236, 487, 1046
BstSFI CTRYAG 1 cut(s) 1082
BstSLI GKGCMC 1 cut(s) 634
BstV1I GCAGC 1 cut(s) 678
BstV2I GAAGAC 2 cut(s) 705, 915
Bsu36I CCTNAGG 1 cut(s) 613
BsuRI GGCC 3 cut(s) 757, 815, 971
BtgI CCRYGG 2 cut(s) 857, 1068
BtgZI GCGATG 1 cut(s) 978
BtsCI GGATG 1 cut(s) 793
BtsIMutI CAGTG 3 cut(s) 351, 634, 849
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 4 cut(s) 436, 809, 813, 973
Cfr10I RCCGGY 1 cut(s) 811
Cfr13I GGNCC 4 cut(s) 608, 756, 772, 969
CseI GACGC 1 cut(s) 242
Csp6I GTAC 4 cut(s) 56, 423, 592, 838
CspCI CAANNNNNGTGG 2 cut(s) 376, 411
CviAII CATG 9 cut(s) 263, 355, 420, 573, 647, 858, 863, 997, 1069
CviQI GTAC 4 cut(s) 56, 423, 592, 838
DdeI CTNAG 2 cut(s) 112, 613
DpnI GATC 5 cut(s) 210, 222, 372, 868, 884
DpnII GATC 5 cut(s) 208, 220, 370, 866, 882
EaeI YGGCCR 1 cut(s) 813
EciI GGCGGA 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 857, 1068
Eco24I GRGCYC 1 cut(s) 959
Eco31I GGTCTC 1 cut(s) 938
Eco32I GATATC 3 cut(s) 277, 910, 1002
Eco47I GGWCC 2 cut(s) 608, 772
Eco57I CTGAAG 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 613
Eco88I CYCGRG 1 cut(s) 916
EcoO109I RGGNCCY 1 cut(s) 756
EcoRII CCWGG 3 cut(s) 63, 487, 1046
EcoRV GATATC 3 cut(s) 277, 910, 1002
EcoT14I CCWWGG 2 cut(s) 857, 1068
EcoT22I ATGCAT 2 cut(s) 465, 574
EcoT38I GRGCYC 1 cut(s) 959
ErhI CCWWGG 2 cut(s) 857, 1068
Esp3I CGTCTC 2 cut(s) 41, 782
FaeI CATG 9 cut(s) 266, 358, 423, 576, 650, 861, 866, 1000, 1072
FaqI GGGAC 2 cut(s) 424, 1029
FatI CATG 9 cut(s) 262, 354, 419, 572, 646, 857, 862, 996, 1068
FauI CCCGC 1 cut(s) 198
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 1 cut(s) 667
FokI GGATG 1 cut(s) 800
FriOI GRGCYC 1 cut(s) 959
Fsp4HI GCNGC 1 cut(s) 667
FspBI CTAG 2 cut(s) 905, 960
GluI GCNGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 3 cut(s) 757, 815, 971
HapII CCGG 5 cut(s) 72, 128, 212, 237, 812
HgaI GACGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 9 cut(s) 266, 358, 423, 576, 650, 861, 866, 1000, 1072
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 8 cut(s) 68, 89, 95, 103, 215, 416, 577, 623
HpaII CCGG 5 cut(s) 72, 128, 212, 237, 812
HphI GGTGA 6 cut(s) 53, 130, 335, 380, 551, 664
Hpy166II GTNNAC 4 cut(s) 562, 632, 798, 847
Hpy188I TCNGA 6 cut(s) 94, 100, 125, 184, 197, 220
Hpy188III TCNNGA 7 cut(s) 31, 212, 224, 272, 290, 615, 870
Hpy8I GTNNAC 4 cut(s) 562, 632, 798, 847
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 4 cut(s) 160, 351, 401, 521
HpyCH4III ACNGT 5 cut(s) 52, 250, 503, 776, 949
HpyCH4IV ACGT 1 cut(s) 1053
HpyCH4V TGCA 6 cut(s) 203, 438, 463, 572, 632, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 435, 808
HpyF3I CTNAG 2 cut(s) 112, 613
HpySE526I ACGT 1 cut(s) 1053
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 9 cut(s) 266, 358, 423, 576, 650, 861, 866, 1000, 1072
Kpn2I TCCGGA 1 cut(s) 211
KpnI GGTACC 2 cut(s) 595, 841
KroI GCCGGC 1 cut(s) 811
KroNI GCCGGC 1 cut(s) 813
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 5 cut(s) 208, 220, 370, 866, 882
LmnI GCTCC 3 cut(s) 185, 198, 650
Lsp1109I GCAGC 1 cut(s) 678
MaeI CTAG 2 cut(s) 905, 960
MaeII ACGT 1 cut(s) 1053
MaeIII GTNAC 2 cut(s) 149, 539
MalI GATC 5 cut(s) 210, 222, 372, 868, 884
MbiI CCGCTC 1 cut(s) 193
MboI GATC 5 cut(s) 208, 220, 370, 866, 882
MboII GAAGA 5 cut(s) 253, 278, 710, 915, 918
MhlI GDGCHC 2 cut(s) 634, 959
MluCI AATT 2 cut(s) 304, 363
MlyI GAGTC 3 cut(s) 89, 209, 410
MmeI TCCRAC 2 cut(s) 220, 805
MnlI CCTC 9 cut(s) 69, 152, 434, 442, 530, 622, 688, 756, 925
Mph1103I ATGCAT 2 cut(s) 465, 574
MroI TCCGGA 1 cut(s) 211
MroNI GCCGGC 1 cut(s) 811
MroXI GAANNNNTTC 1 cut(s) 622
MslI CAYNNNNRTG 3 cut(s) 156, 228, 1067
MspI CCGG 5 cut(s) 72, 128, 212, 237, 812
MspR9I CCNGG 5 cut(s) 65, 129, 238, 489, 1048
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 3 cut(s) 65, 489, 1048
MwoI GCNNNNNNNGC 2 cut(s) 435, 808
NaeI GCCGGC 1 cut(s) 813
NciI CCSGG 2 cut(s) 129, 238
NcoI CCATGG 2 cut(s) 857, 1068
NdeII GATC 5 cut(s) 208, 220, 370, 866, 882
NgoMIV GCCGGC 1 cut(s) 811
NlaIII CATG 9 cut(s) 266, 358, 423, 576, 650, 861, 866, 1000, 1072
NlaIV GGNNCC 5 cut(s) 181, 593, 610, 839, 970
NmuCI GTSAC 2 cut(s) 149, 539
NsiI ATGCAT 2 cut(s) 465, 574
PctI GAATGC 1 cut(s) 830
PdiI GCCGGC 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 5 cut(s) 68, 89, 103, 577, 623
PflMI CCANNNNNTGG 1 cut(s) 863
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 1 cut(s) 668
PleI GAGTC 3 cut(s) 89, 209, 410
PpsI GAGTC 3 cut(s) 89, 209, 410
Psp1406I AACGTT 1 cut(s) 1053
Psp6I CCWGG 3 cut(s) 63, 487, 1046
PspGI CCWGG 3 cut(s) 63, 487, 1046
PspN4I GGNNCC 5 cut(s) 181, 593, 610, 839, 970
PspPI GGNCC 4 cut(s) 608, 756, 772, 969
RsaI GTAC 4 cut(s) 57, 424, 593, 839
RsaNI GTAC 4 cut(s) 56, 423, 592, 838
RseI CAYNNNNRTG 3 cut(s) 156, 228, 1067
SatI GCNGC 1 cut(s) 667
Sau3AI GATC 5 cut(s) 208, 220, 370, 866, 882
Sau96I GGNCC 4 cut(s) 608, 756, 772, 969
SchI GAGTC 3 cut(s) 89, 209, 410
ScrFI CCNGG 5 cut(s) 65, 129, 238, 489, 1048
SduI GDGCHC 2 cut(s) 634, 959
SfcI CTRYAG 1 cut(s) 1082
SinI GGWCC 2 cut(s) 608, 772
SmiMI CAYNNNNRTG 3 cut(s) 156, 228, 1067
SmlI CTYRAG 1 cut(s) 760
SmoI CTYRAG 1 cut(s) 760
Sse9I AATT 2 cut(s) 304, 363
SsiI CCGC 3 cut(s) 44, 191, 902
SspMI CTAG 2 cut(s) 905, 960
StyD4I CCNGG 5 cut(s) 63, 127, 236, 487, 1046
StyI CCWWGG 2 cut(s) 857, 1068
TaaI ACNGT 5 cut(s) 52, 250, 503, 776, 949
TaiI ACGT 1 cut(s) 1056
TaqI TCGA 3 cut(s) 144, 271, 447
TasI AATT 2 cut(s) 304, 363
TfiI GAWTC 5 cut(s) 68, 89, 103, 577, 623
TscAI CASTG 3 cut(s) 351, 634, 849
TseFI GTSAC 2 cut(s) 149, 539
TseI GCWGC 1 cut(s) 666
Tsp45I GTSAC 2 cut(s) 149, 539
TspGWI ACGGA 1 cut(s) 814
TspRI CASTG 3 cut(s) 351, 634, 849
Van91I CCANNNNNTGG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 630
VpaK11BI GGWCC 2 cut(s) 608, 772
XmnI GAANNNNTTC 1 cut(s) 622
XspI CTAG 2 cut(s) 905, 960
Zsp2I ATGCAT 2 cut(s) 465, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.