Prupe.8G000200_v2.0.a1

Ethylbenzene dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0010461
Physical Location & Seq
Reverse (-)
45920 .. 49318
3399 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G000200.1

Sequence Viewer

Length: 1083 bp
ATGCTTCGAGTAGTGCTAGTGCTATTGGTATTTTGTGTGCTGCTGATACGACAGGCCAATTCGCACGAAGAGTCGGGTGAATGGAGCTGTGAGTCGGACTCTGAGATCCGGGTCCAGGCCGAGTTCGGGCCCGGCCTTATTACGCTCGATGGTCATGCCGACGACTGGAAGGACATGGATGGGTTCGAGTTCTCTCTCCGCCCTGCTCTTGACCCAGATGCTGAAAACGAGTACAGAGGTGGAAAAATGACAGTTAAGGCTTTGCATGATGGCCGTGATGTCTTCTTTATGGTTCAAGTTGATGGGGACTACGCTTACTCTAAGGGTGACAACAATAAATGCCCATCTGTTGCCCTCATGTTTCAAATTGGTGAAGATGCAACTTATCATAGTATGGGTGGCTGTAAGGAAGGAGTTGAGGCTTGCACAAACAAGACGTGCAAGGGCCATGAAGTTGATATATTGCACTTCTCAATTGGGAATGCTATTCCTGGACGGCTCTATGGTGGCAACCCTGTAGACAACAGGGTTGGAAATGGAGGCGACAGGTTTGGTATTTTAGTTGATCTTTATGCTTGGAATCCGCATTGTAGATACCTAGATGGAATTGGTTCTTCAGCAAATGATTCTAGTGGACAAAATGATTGGAAGGGTGCTTGGTGGCACAGTAGCTTCAACGTTCACTCAGGTTTCGTGGAAGAAGACAGTCCTTATGCATCAGATGGCCAAAAGGGAACATTTTATTTTGAATTCTCTAGGCCTTTGAGAACTATGGATCGTCTCCAACAGGATGTGCAATTCACAATTGGAGGATCAAGTAAAATGTCAGTTGCATTTTGGTATCCAGTTGATGGAAAACCGTGGCACGGCTCTGGACACTATTCTGTTAGCTGTGATTGGGTCCCCTTAGACATCTCTTTGGGAAGTTCTGTGTTTACCATGGCAAGACCACGTAGCTCAGTCAGTGCTGCTAGTGTTTTTGCCCTTTTGTTATCAGTAATCTCATTATGTGCTTCCGTTTTTATTGGATATTGGGTCGTTAAACCCCAAACTGCCCAGTTCACACCCATGGAAAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EB_dh

Protein Analysis

361

Amino Acids

39.73

Weight (kDa)

4.97

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 851
AccI GTMKAC 1 cut(s) 519
AciI CCGC 2 cut(s) 199, 584
AclI AACGTT 1 cut(s) 678
AclWI GGATC 3 cut(s) 100, 783, 820
AcoI YGGCCR 2 cut(s) 271, 724
AcsI RAATTY 1 cut(s) 749
AcuI CTGAAG 1 cut(s) 600
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 5 cut(s) 115, 126, 165, 851, 866
AgsI TTSAA 4 cut(s) 296, 365, 676, 749
AjiI CACGTC 1 cut(s) 438
AjnI CCWGG 2 cut(s) 114, 490
AluBI AGCT 4 cut(s) 87, 672, 891, 957
AluI AGCT 4 cut(s) 87, 672, 891, 957
Alw26I GTCTC 1 cut(s) 785
AlwI GGATC 3 cut(s) 100, 783, 820
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 8 cut(s) 54, 117, 128, 133, 271, 445, 724, 758
ApaI GGGCCC 1 cut(s) 132
ApeKI GCWGC 2 cut(s) 40, 968
ApoI RAATTY 1 cut(s) 749
Asp700I GAANNNNTTC 1 cut(s) 610
AspS9I GGNCC 5 cut(s) 112, 128, 129, 445, 901
AsuC2I CCSGG 2 cut(s) 110, 132
AsuHPI GGTGA 3 cut(s) 89, 338, 383
AvaII GGWCC 2 cut(s) 112, 901
BaeGI GKGCMC 1 cut(s) 132
BalI TGGCCA 1 cut(s) 726
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 2 cut(s) 274, 708
BbvI GCAGC 2 cut(s) 27, 955
BccI CCATC 8 cut(s) 143, 173, 263, 296, 352, 596, 716, 845
BceAI ACGGC 3 cut(s) 258, 512, 883
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 2 cut(s) 116, 492
BciVI GTATCC 1 cut(s) 852
BcnI CCSGG 2 cut(s) 110, 132
BcoDI GTCTC 1 cut(s) 785
BfaI CTAG 5 cut(s) 17, 599, 630, 756, 972
BfmI CTRYAG 1 cut(s) 516
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 2 cut(s) 41, 969
BlsI GCNGC 2 cut(s) 42, 970
Bme1390I CCNGG 4 cut(s) 110, 116, 132, 492
Bme18I GGWCC 2 cut(s) 112, 901
BmgBI CACGTC 1 cut(s) 438
BmgT120I GGNCC 5 cut(s) 112, 128, 129, 445, 901
BmiI GGNNCC 4 cut(s) 113, 130, 902, 903
BmrFI CCNGG 4 cut(s) 110, 116, 132, 492
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 3 cut(s) 208, 367, 725
BmuI ACTGGG 1 cut(s) 1051
BpiI GAAGAC 2 cut(s) 274, 708
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpuMI CCSGG 2 cut(s) 110, 132
BsaAI YACGTR 1 cut(s) 953
BsaJI CCNNGG 3 cut(s) 860, 939, 1068
Bsc4I CCNNNNNNNGG 5 cut(s) 115, 126, 165, 851, 866
Bse1I ACTGG 3 cut(s) 170, 845, 1057
BseBI CCWGG 2 cut(s) 116, 492
BseDI CCNNGG 3 cut(s) 860, 939, 1068
BseGI GGATG 2 cut(s) 184, 796
BseLI CCNNNNNNNGG 5 cut(s) 115, 126, 165, 851, 866
BseMII CTCAG 3 cut(s) 93, 699, 972
BseNI ACTGG 3 cut(s) 170, 845, 1057
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 2 cut(s) 27, 955
BshFI GGCC 8 cut(s) 56, 119, 130, 135, 273, 447, 726, 760
BsiSI CCGG 2 cut(s) 109, 132
BslFI GGGAC 2 cut(s) 320, 887
BslI CCNNNNNNNGG 5 cut(s) 115, 126, 165, 851, 866
BsmAI GTCTC 1 cut(s) 785
BsmBI CGTCTC 1 cut(s) 785
BsmFI GGGAC 2 cut(s) 320, 887
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 8 cut(s) 56, 119, 130, 135, 273, 447, 726, 760
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 4 cut(s) 105, 565, 775, 812
Bsp19I CCATGG 2 cut(s) 939, 1068
BspACI CCGC 2 cut(s) 199, 584
BspANI GGCC 8 cut(s) 56, 119, 130, 135, 273, 447, 726, 760
BspCNI CTCAG 3 cut(s) 94, 698, 971
BspLI GGNNCC 4 cut(s) 113, 130, 902, 903
BspPI GGATC 3 cut(s) 100, 783, 820
BsrI ACTGG 3 cut(s) 170, 845, 1057
BssECI CCNNGG 3 cut(s) 860, 939, 1068
BssMI GATC 4 cut(s) 105, 565, 775, 812
BssT1I CCWWGG 2 cut(s) 939, 1068
Bst2UI CCWGG 2 cut(s) 116, 492
Bst4CI ACNGT 4 cut(s) 253, 668, 707, 861
Bst6I CTCTTC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 953
BstC8I GCNNGC 1 cut(s) 424
BstDEI CTNAG 5 cut(s) 102, 321, 685, 907, 958
BstDSI CCRYGG 3 cut(s) 860, 939, 1068
BstF5I GGATG 2 cut(s) 184, 796
BstKTI GATC 4 cut(s) 108, 568, 778, 815
BstMAI GTCTC 1 cut(s) 785
BstMBI GATC 4 cut(s) 105, 565, 775, 812
BstNI CCWGG 2 cut(s) 116, 492
BstSCI CCNGG 4 cut(s) 108, 114, 130, 490
BstSFI CTRYAG 1 cut(s) 516
BstSLI GKGCMC 1 cut(s) 132
BstV1I GCAGC 2 cut(s) 27, 955
BstV2I GAAGAC 2 cut(s) 274, 708
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 8 cut(s) 56, 119, 130, 135, 273, 447, 726, 760
BtgI CCRYGG 3 cut(s) 860, 939, 1068
BtrI CACGTC 1 cut(s) 438
BtsCI GGATG 2 cut(s) 184, 796
BtsIMutI CAGTG 1 cut(s) 970
Cac8I GCNNGC 1 cut(s) 424
CaiI CAGNNNCTG 1 cut(s) 221
Cfr13I GGNCC 5 cut(s) 112, 128, 129, 445, 901
Csp6I GTAC 1 cut(s) 232
CviAII CATG 7 cut(s) 155, 175, 266, 358, 449, 940, 1069
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 5 cut(s) 102, 321, 685, 907, 958
DpnI GATC 4 cut(s) 107, 567, 777, 814
DpnII GATC 4 cut(s) 105, 565, 775, 812
EaeI YGGCCR 2 cut(s) 271, 724
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
EciI GGCGGA 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 939, 1068
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 112, 901
Eco57I CTGAAG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 901
EcoRI GAATTC 1 cut(s) 749
EcoRII CCWGG 2 cut(s) 114, 490
EcoT14I CCWWGG 2 cut(s) 939, 1068
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 939, 1068
Esp3I CGTCTC 1 cut(s) 785
FaeI CATG 7 cut(s) 158, 178, 269, 361, 452, 943, 1072
FaqI GGGAC 2 cut(s) 320, 887
FatI CATG 7 cut(s) 154, 174, 265, 357, 448, 939, 1068
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 2 cut(s) 41, 969
FokI GGATG 2 cut(s) 191, 803
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 2 cut(s) 41, 969
FspBI CTAG 5 cut(s) 17, 599, 630, 756, 972
GluI GCNGC 2 cut(s) 41, 969
HaeIII GGCC 8 cut(s) 56, 119, 130, 135, 273, 447, 726, 760
HapII CCGG 2 cut(s) 109, 132
Hin1II CATG 7 cut(s) 158, 178, 269, 361, 452, 943, 1072
HinfI GANTC 5 cut(s) 71, 92, 98, 580, 626
HpaII CCGG 2 cut(s) 109, 132
HphI GGTGA 3 cut(s) 89, 338, 383
Hpy166II GTNNAC 5 cut(s) 520, 635, 682, 936, 1062
Hpy188I TCNGA 3 cut(s) 97, 103, 721
Hpy188III TCNNGA 2 cut(s) 209, 873
Hpy8I GTNNAC 5 cut(s) 520, 635, 682, 936, 1062
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 163, 404, 643
HpyCH4III ACNGT 4 cut(s) 253, 668, 707, 861
HpyCH4IV ACGT 3 cut(s) 437, 678, 952
HpyCH4V TGCA 8 cut(s) 265, 380, 426, 441, 466, 716, 796, 833
HpyF3I CTNAG 5 cut(s) 102, 321, 685, 907, 958
HpySE526I ACGT 3 cut(s) 437, 678, 952
Hsp92II CATG 7 cut(s) 158, 178, 269, 361, 452, 943, 1072
KflI GGGWCCC 1 cut(s) 901
Kzo9I GATC 4 cut(s) 105, 565, 775, 812
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 2 cut(s) 27, 955
LweI GCATC 3 cut(s) 208, 367, 725
MaeI CTAG 5 cut(s) 17, 599, 630, 756, 972
MaeII ACGT 3 cut(s) 437, 678, 952
MaeIII GTNAC 1 cut(s) 326
MalI GATC 4 cut(s) 107, 567, 777, 814
MboI GATC 4 cut(s) 105, 565, 775, 812
MboII GAAGA 6 cut(s) 80, 274, 386, 606, 710, 713
MfeI CAATTG 2 cut(s) 474, 804
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 1 cut(s) 132
MlsI TGGCCA 1 cut(s) 726
MluCI AATT 7 cut(s) 58, 366, 474, 606, 749, 797, 804
MluNI TGGCCA 1 cut(s) 726
MlyI GAGTC 3 cut(s) 80, 92, 101
MmeI TCCRAC 3 cut(s) 75, 511, 808
MnlI CCTC 5 cut(s) 230, 365, 412, 533, 803
Mox20I TGGCCA 1 cut(s) 726
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 610
MscI TGGCCA 1 cut(s) 726
MseI TTAA 3 cut(s) 255, 1041, 1081
MslI CAYNNNNRTG 1 cut(s) 1067
Msp20I TGGCCA 1 cut(s) 726
MspI CCGG 2 cut(s) 109, 132
MspR9I CCNGG 4 cut(s) 110, 116, 132, 492
MunI CAATTG 2 cut(s) 474, 804
Mva1269I GAATGC 1 cut(s) 487
MvaI CCWGG 2 cut(s) 116, 492
NciI CCSGG 2 cut(s) 110, 132
NcoI CCATGG 2 cut(s) 939, 1068
NdeII GATC 4 cut(s) 105, 565, 775, 812
NlaIII CATG 7 cut(s) 158, 178, 269, 361, 452, 943, 1072
NlaIV GGNNCC 4 cut(s) 113, 130, 902, 903
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 718
PceI AGGCCT 1 cut(s) 760
PctI GAATGC 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 2 cut(s) 580, 626
PflMI CCANNNNNTGG 1 cut(s) 851
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 2 cut(s) 42, 970
PleI GAGTC 3 cut(s) 79, 92, 100
PpsI GAGTC 3 cut(s) 79, 92, 100
Ppu21I YACGTR 1 cut(s) 953
PpuMI RGGWCCY 1 cut(s) 901
Psp1406I AACGTT 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 901
Psp6I CCWGG 2 cut(s) 114, 490
PspGI CCWGG 2 cut(s) 114, 490
PspN4I GGNNCC 4 cut(s) 113, 130, 902, 903
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 5 cut(s) 112, 128, 129, 445, 901
PspPPI RGGWCCY 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 221
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 1067
SaqAI TTAA 3 cut(s) 255, 1041, 1081
SatI GCNGC 2 cut(s) 41, 969
Sau3AI GATC 4 cut(s) 105, 565, 775, 812
Sau96I GGNCC 5 cut(s) 112, 128, 129, 445, 901
SchI GAGTC 3 cut(s) 80, 92, 101
ScrFI CCNGG 4 cut(s) 110, 116, 132, 492
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 3 cut(s) 208, 367, 725
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 2 cut(s) 112, 901
SmiMI CAYNNNNRTG 1 cut(s) 1067
Sse9I AATT 7 cut(s) 58, 366, 474, 606, 749, 797, 804
SseBI AGGCCT 1 cut(s) 760
SsiI CCGC 2 cut(s) 199, 584
SspMI CTAG 5 cut(s) 17, 599, 630, 756, 972
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 4 cut(s) 108, 114, 130, 490
StyI CCWWGG 2 cut(s) 939, 1068
TaaI ACNGT 4 cut(s) 253, 668, 707, 861
TaiI ACGT 3 cut(s) 440, 681, 955
TaqI TCGA 3 cut(s) 7, 147, 186
TasI AATT 7 cut(s) 58, 366, 474, 606, 749, 797, 804
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 2 cut(s) 580, 626
Tru1I TTAA 3 cut(s) 255, 1041, 1081
Tru9I TTAA 3 cut(s) 255, 1041, 1081
TscAI CASTG 1 cut(s) 970
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 2 cut(s) 40, 968
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 465
TspGWI ACGGA 1 cut(s) 1006
TspRI CASTG 1 cut(s) 970
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 2 cut(s) 112, 901
XapI RAATTY 1 cut(s) 749
XmiI GTMKAC 1 cut(s) 519
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 5 cut(s) 17, 599, 630, 756, 972
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.