Rroxscaffold_7G00217880

Ethylbenzene dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010461
Physical Location & Seq
Forward (+)
68445637 .. 68450565
4929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217880.1

Sequence Viewer

Length: 1110 bp
ATGCTTCGACCATGGCTACTGCTGTTAGTATTTTGTGTGCCTCTGCTACAACGGGTCAACTCGCACGAAGAGTCGGGTGAATGGAGCTGCGAGTCCGACTCAGACACCCGAATCCAGGCCCAGTTCGGGCCCGGCCTTATCACCCTCGATGGTCACGCCGACGATTGGAAGGACACGGACGGGTCCGAGTTCTCACTTCTCCCTGCTCTTGACCCAGATGCTGAAACCGAATACACAGGTGGAAAGATGACCGTCAAGGCTTTGCATGATGGCCGTGATGTCTTCTTTATGGTTCAAGTTGATGGGGATTACGCTTACTCAAAAGGTGAGAATACCAAATGCCCATCTGTTGCCCTCATGTTTCAAATTGGCGAAGCTGCAACTTATCATAATATGGGCGGCTGTAAGGAAGGAGTTGACTCTTGCACAGACAAGACTTGCAAATCCCATGAAGTTGACATTATGCATTTCTCTATTGGAAATGCAATTCCTGGACGTCTCTATGGTGGTAACCCAGTGGACAACAGGGATGGAAATGGAGGTGACAGGTTTGGTCATTTGAATGATCTTTATGGTTGGAACCCACACTGTAGATACCTAGATGGAATTAGCTCTTCAGGTATTCCTAAGGCTATTTATCCTACTGCAAATGATTCTAGTGCACAAAATGATTGGAAAGGCGCTTGGTGGCACAGTAGCTTGAATGTTCACTCAGGTTTTGTGGAAGAAGATAGTCCTTATGCATCAGATGGTCAAAAGGGTACATACTATTTTGAATTCTCTAGGCCTTTGAGAACTATGGATCGCCTCCAACAGGATGCTCAATTCACAATTGGCGGATCAAGTAAGATGTCAGTTGCATTCTGGTATCCTGTTGATGGAAAACCATGGCATGGCTCTGGACACTATTCTGTTAGCTGTGACTGGGTTCCCTTGGATATATCTATGAAAATCTCTATGCTAACAAAGGTGAAACAAAGTAGTTCAGTCAGTGCTGCTAGTGTTTTTGCTCTTTTGCTATCAGTAATCTCATTATGTGTTTCTGTTTTTATCGGGTATTGGGTTGTCAAACCCAAAACTATCCCCTTCACACCCATGGAAAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EB_dh

Protein Analysis

369

Amino Acids

40.75

Weight (kDa)

5.09

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EB_dh PF09459 54 - 304 4.2e-36 Ethylbenzene dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 893
AciI CCGC 2 cut(s) 399, 837
AclWI GGATC 2 cut(s) 810, 847
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 1 cut(s) 776
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 1 cut(s) 763
AfiI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 814, 878, 893
AgsI TTSAA 5 cut(s) 296, 365, 562, 703, 776
AjnI CCWGG 2 cut(s) 114, 490
AluBI AGCT 5 cut(s) 87, 377, 612, 699, 918
AluI AGCT 5 cut(s) 87, 377, 612, 699, 918
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 1 cut(s) 503
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 2 cut(s) 810, 847
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 5 cut(s) 117, 128, 133, 271, 785
ApaI GGGCCC 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 3 cut(s) 87, 377, 995
ApoI RAATTY 1 cut(s) 776
AspLEI GCGC 1 cut(s) 683
AspS9I GGNCC 4 cut(s) 118, 128, 129, 183
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 5 cut(s) 89, 133, 338, 554, 982
AvaII GGWCC 1 cut(s) 183
AxyI CCTNAGG 1 cut(s) 627
BaeGI GKGCMC 2 cut(s) 132, 664
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 74, 364, 982
BccI CCATC 8 cut(s) 143, 263, 296, 352, 524, 596, 743, 872
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 2 cut(s) 116, 492
BciVI GTATCC 1 cut(s) 879
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 503
BfaI CTAG 4 cut(s) 599, 657, 783, 999
BfmI CTRYAG 1 cut(s) 589
BfoI RGCGCY 1 cut(s) 684
BfuI GTATCC 1 cut(s) 879
BisI GCNGC 4 cut(s) 88, 378, 400, 996
BlsI GCNGC 4 cut(s) 89, 379, 401, 997
Bme1390I CCNGG 3 cut(s) 116, 132, 492
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 4 cut(s) 118, 128, 129, 183
BmiI GGNNCC 4 cut(s) 130, 184, 581, 930
BmrFI CCNGG 3 cut(s) 116, 132, 492
BmrI ACTGGG 3 cut(s) 115, 509, 934
BmsI GCATC 3 cut(s) 208, 752, 808
BmuI ACTGGG 3 cut(s) 115, 509, 934
BpiI GAAGAC 1 cut(s) 274
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpuMI CCSGG 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 4 cut(s) 11, 887, 933, 1095
Bsc4I CCNNNNNNNGG 6 cut(s) 115, 126, 165, 814, 878, 893
Bse1I ACTGG 3 cut(s) 121, 515, 929
Bse21I CCTNAGG 1 cut(s) 627
BseBI CCWGG 2 cut(s) 116, 492
BseDI CCNNGG 4 cut(s) 11, 887, 933, 1095
BseGI GGATG 2 cut(s) 535, 823
BseLI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 814, 878, 893
BseMII CTCAG 2 cut(s) 114, 726
BseNI ACTGG 3 cut(s) 121, 515, 929
BseSI GKGCMC 2 cut(s) 132, 664
BseXI GCAGC 3 cut(s) 74, 364, 982
BshFI GGCC 5 cut(s) 119, 130, 135, 273, 787
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 814, 878, 893
BsmAI GTCTC 1 cut(s) 503
BsmBI CGTCTC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 860
BsnI GGCC 5 cut(s) 119, 130, 135, 273, 787
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 132, 664
Bsp143I GATC 3 cut(s) 565, 802, 839
Bsp19I CCATGG 3 cut(s) 11, 887, 1095
BspACI CCGC 2 cut(s) 399, 837
BspANI GGCC 5 cut(s) 119, 130, 135, 273, 787
BspCNI CTCAG 2 cut(s) 113, 725
BspLI GGNNCC 4 cut(s) 130, 184, 581, 930
BspPI GGATC 2 cut(s) 810, 847
BspQI GCTCTTC 1 cut(s) 619
BsrI ACTGG 3 cut(s) 121, 515, 929
BssECI CCNNGG 4 cut(s) 11, 887, 933, 1095
BssMI GATC 3 cut(s) 565, 802, 839
BssNI GRCGYC 1 cut(s) 496
BssT1I CCWWGG 4 cut(s) 11, 887, 933, 1095
Bst2UI CCWGG 2 cut(s) 116, 492
Bst4CI ACNGT 3 cut(s) 253, 590, 695
Bst6I CTCTTC 2 cut(s) 63, 619
BstACI GRCGYC 1 cut(s) 496
BstDEI CTNAG 3 cut(s) 100, 627, 712
BstDSI CCRYGG 3 cut(s) 11, 887, 1095
BstEII GGTNACC 1 cut(s) 509
BstENI CCTNNNNNAGG 1 cut(s) 812
BstF5I GGATG 2 cut(s) 535, 823
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 1 cut(s) 683
BstKTI GATC 3 cut(s) 568, 805, 842
BstMAI GTCTC 1 cut(s) 503
BstMBI GATC 3 cut(s) 565, 802, 839
BstNI CCWGG 2 cut(s) 116, 492
BstPI GGTNACC 1 cut(s) 509
BstSCI CCNGG 3 cut(s) 114, 130, 490
BstSFI CTRYAG 1 cut(s) 589
BstSLI GKGCMC 2 cut(s) 132, 664
BstV1I GCAGC 3 cut(s) 74, 364, 982
BstV2I GAAGAC 1 cut(s) 274
Bsu36I CCTNAGG 1 cut(s) 627
BsuI GTATCC 1 cut(s) 879
BsuRI GGCC 5 cut(s) 119, 130, 135, 273, 787
BtgI CCRYGG 3 cut(s) 11, 887, 1095
BtsCI GGATG 2 cut(s) 535, 823
BtsIMutI CAGTG 3 cut(s) 522, 586, 997
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 1 cut(s) 683
Cfr13I GGNCC 4 cut(s) 118, 128, 129, 183
Csp6I GTAC 1 cut(s) 762
CviAII CATG 7 cut(s) 12, 266, 358, 449, 888, 893, 1096
CviQI GTAC 1 cut(s) 762
DdeI CTNAG 3 cut(s) 100, 627, 712
DpnI GATC 3 cut(s) 567, 804, 841
DpnII GATC 3 cut(s) 565, 802, 839
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 63, 619
EarI CTCTTC 2 cut(s) 63, 619
EciI GGCGGA 1 cut(s) 852
Eco130I CCWWGG 4 cut(s) 11, 887, 933, 1095
Eco147I AGGCCT 1 cut(s) 787
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 600
Eco81I CCTNAGG 1 cut(s) 627
Eco91I GGTNACC 1 cut(s) 509
EcoNI CCTNNNNNAGG 1 cut(s) 812
EcoO65I GGTNACC 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 776
EcoRII CCWGG 2 cut(s) 114, 490
EcoT14I CCWWGG 4 cut(s) 11, 887, 933, 1095
EcoT22I ATGCAT 2 cut(s) 468, 745
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 4 cut(s) 11, 887, 933, 1095
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 7 cut(s) 15, 269, 361, 452, 891, 896, 1099
FatI CATG 7 cut(s) 11, 265, 357, 448, 887, 892, 1095
Fnu4HI GCNGC 4 cut(s) 88, 378, 400, 996
FokI GGATG 2 cut(s) 542, 830
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 4 cut(s) 88, 378, 400, 996
FspBI CTAG 4 cut(s) 599, 657, 783, 999
GlaI GCGC 1 cut(s) 682
GluI GCNGC 4 cut(s) 88, 378, 400, 996
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 5 cut(s) 119, 130, 135, 273, 787
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 683
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 7 cut(s) 15, 269, 361, 452, 891, 896, 1099
Hin6I GCGC 1 cut(s) 681
HinP1I GCGC 1 cut(s) 681
HincII GTYRAC 3 cut(s) 58, 418, 457
HindII GTYRAC 3 cut(s) 58, 418, 457
HinfI GANTC 6 cut(s) 71, 92, 98, 111, 419, 653
HpaII CCGG 1 cut(s) 132
HphI GGTGA 5 cut(s) 89, 133, 338, 554, 982
Hpy166II GTNNAC 6 cut(s) 58, 418, 457, 520, 662, 709
Hpy188I TCNGA 4 cut(s) 97, 103, 187, 748
Hpy188III TCNNGA 2 cut(s) 209, 900
Hpy8I GTNNAC 6 cut(s) 58, 418, 457, 520, 662, 709
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 163, 404, 1096
HpyCH4III ACNGT 3 cut(s) 253, 590, 695
HpyCH4IV ACGT 1 cut(s) 496
HpyF3I CTNAG 3 cut(s) 100, 627, 712
HpySE526I ACGT 1 cut(s) 496
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 7 cut(s) 15, 269, 361, 452, 891, 896, 1099
HspAI GCGC 1 cut(s) 681
Kzo9I GATC 3 cut(s) 565, 802, 839
LguI GCTCTTC 1 cut(s) 619
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 3 cut(s) 74, 364, 982
LweI GCATC 3 cut(s) 208, 752, 808
MaeI CTAG 4 cut(s) 599, 657, 783, 999
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 4 cut(s) 152, 509, 542, 920
MalI GATC 3 cut(s) 567, 804, 841
MboI GATC 3 cut(s) 565, 802, 839
MboII GAAGA 5 cut(s) 80, 274, 606, 737, 740
MfeI CAATTG 1 cut(s) 831
MhlI GDGCHC 2 cut(s) 132, 664
MluCI AATT 6 cut(s) 366, 486, 606, 776, 824, 831
MlyI GAGTC 4 cut(s) 80, 92, 101, 413
MmeI TCCRAC 3 cut(s) 120, 557, 835
MnlI CCTC 5 cut(s) 51, 155, 365, 533, 818
Mph1103I ATGCAT 2 cut(s) 468, 745
MseI TTAA 1 cut(s) 1108
MslI CAYNNNNRTG 2 cut(s) 561, 1094
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 116, 132, 492
MunI CAATTG 1 cut(s) 831
Mva1269I GAATGC 1 cut(s) 860
MvaI CCWGG 2 cut(s) 116, 492
NciI CCSGG 1 cut(s) 132
NcoI CCATGG 3 cut(s) 11, 887, 1095
NdeII GATC 3 cut(s) 565, 802, 839
NlaIII CATG 7 cut(s) 15, 269, 361, 452, 891, 896, 1099
NlaIV GGNNCC 4 cut(s) 130, 184, 581, 930
NmuCI GTSAC 3 cut(s) 152, 542, 920
NsiI ATGCAT 2 cut(s) 468, 745
PceI AGGCCT 1 cut(s) 787
PciSI GCTCTTC 1 cut(s) 619
PctI GAATGC 1 cut(s) 860
PfeI GAWTC 2 cut(s) 111, 653
PflMI CCANNNNNTGG 1 cut(s) 893
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 4 cut(s) 89, 379, 401, 997
PleI GAGTC 4 cut(s) 79, 92, 100, 413
PpsI GAGTC 4 cut(s) 79, 92, 100, 413
Psp6I CCWGG 2 cut(s) 114, 490
PspEI GGTNACC 1 cut(s) 509
PspGI CCWGG 2 cut(s) 114, 490
PspN4I GGNNCC 4 cut(s) 130, 184, 581, 930
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 4 cut(s) 118, 128, 129, 183
PstNI CAGNNNCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 763
RsaNI GTAC 1 cut(s) 762
RseI CAYNNNNRTG 2 cut(s) 561, 1094
SapI GCTCTTC 1 cut(s) 619
SaqAI TTAA 1 cut(s) 1108
SatI GCNGC 4 cut(s) 88, 378, 400, 996
Sau3AI GATC 3 cut(s) 565, 802, 839
Sau96I GGNCC 4 cut(s) 118, 128, 129, 183
SchI GAGTC 4 cut(s) 80, 92, 101, 413
ScrFI CCNGG 3 cut(s) 116, 132, 492
SduI GDGCHC 2 cut(s) 132, 664
SfaNI GCATC 3 cut(s) 208, 752, 808
SfcI CTRYAG 1 cut(s) 589
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 2 cut(s) 561, 1094
Sse9I AATT 6 cut(s) 366, 486, 606, 776, 824, 831
SseBI AGGCCT 1 cut(s) 787
SsiI CCGC 2 cut(s) 399, 837
SspMI CTAG 4 cut(s) 599, 657, 783, 999
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 3 cut(s) 114, 130, 490
StyI CCWWGG 4 cut(s) 11, 887, 933, 1095
TaaI ACNGT 3 cut(s) 253, 590, 695
TaiI ACGT 1 cut(s) 499
TaqI TCGA 2 cut(s) 7, 147
TasI AATT 6 cut(s) 366, 486, 606, 776, 824, 831
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 2 cut(s) 111, 653
Tru1I TTAA 1 cut(s) 1108
Tru9I TTAA 1 cut(s) 1108
TscAI CASTG 3 cut(s) 522, 593, 997
TseFI GTSAC 3 cut(s) 152, 542, 920
TseI GCWGC 3 cut(s) 87, 377, 995
Tsp45I GTSAC 3 cut(s) 152, 542, 920
TspDTI ATGAA 2 cut(s) 465, 962
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 3 cut(s) 522, 593, 997
Van91I CCANNNNNTGG 1 cut(s) 893
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 183
XagI CCTNNNNNAGG 1 cut(s) 812
XapI RAATTY 1 cut(s) 776
XspI CTAG 4 cut(s) 599, 657, 783, 999
ZraI GACGTC 1 cut(s) 497
Zsp2I ATGCAT 2 cut(s) 468, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.