FvH4_2g00030

Ethylbenzene dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010461
Physical Location & Seq
Reverse (-)
99150 .. 102572
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00030.t2

Sequence Viewer

Length: 1086 bp
ATGCTTCGACTATGGCTATTGCTACTAGTACTTTTTGTGCCTCTGCTACGGCGGGTCAACTCGCACGAAGAGTCGGGTGAATGGAGCTGCGAGTCCGACTCAGGCATCCGAATCCAGGCCCAGTTTGGGCCCGGCCTTATCACCCTCGACGGTCACGCCGACGATTGGAAGGACATGGATGGGTCCGAGTTCTCTCTTCTCCCTGCTCTTGATCCAGATGCTGAAAACGAATACGCAGCTGGAAAGATGACCCTCAAGGCTTTGCATGATGGCCGTGATGTCTTCTTTATGGTTCAAGTTGATGGGGATTACGCTTACTCAAAAGGTGAGAATAACAAATGCCCATCTGTTGCCCTCATGTTTCAAATTGGTGAAGCTGCAACTTATCATAATATGGGCGGCTGTAAGGAAGGAGTTGACTCTTGCACAAACAAGACTTGCAAAACCCATGAAGTTGACATTATGCACTTCTCTATTGGAAATGCAATTCCTGGACGTCTCTATGGTGGCAACCCAATGGACAACAGGGATGGAAATGGAGGTGACAGCAGGTTTGGTCATTTGGTTGATCTTTATGCTTGGAACCCACACTGTAGATACCTAGATGGAATTAGCTCTTCAGCAAATGATTCTAGTGCGCAAAATGATTGGAAAGGCGCTTGGTGGCACAGTAGCTTGAATGTTCACTCAGGTTTTGTGGAAGAAGATAGTCCTTATGCATCAGATGGTCAGAAGGGTACATTTTATTTTGAATTCTCTAGGCCTTTGAGAACTATGGATCGCCTCCAGCAGGATGCTCAATTCACAATTGGTGGATCAAGTAAGATGTCAGTTGCATTCTGGTATCCTGTTGATGGAAAACCATGGCATGGGTCTGGACACTATTCTGTTAGCTGTGACTGGGTTCCCTTGGATATATCTTTGAAAACCTCAATGCTAACAAAGGTGAGACAAGGAAGTTCAGTCAGTGCTGCTAGTGTTTTTGCCCTTTTGCTATCTGTAATCTCATTATGTGCTTCTGTCTTTATTGGGTATTGGGTTGTCAGACCCAAAACTATCCCCTTCACACCCATGGAAAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EB_dh

Protein Analysis

362

Amino Acids

39.96

Weight (kDa)

5.24

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EB_dh PF09459 8 - 199 1.2e-29 Ethylbenzene dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 499
Acc16I TGCGCA 1 cut(s) 639
Acc36I ACCTGC 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 869
AciI CCGC 2 cut(s) 52, 399
AclWI GGATC 3 cut(s) 206, 786, 823
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 1 cut(s) 752
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 2 cut(s) 30, 739
AfiI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 790, 854, 869
AgsI TTSAA 5 cut(s) 296, 365, 679, 752, 925
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 2 cut(s) 114, 490
AluBI AGCT 6 cut(s) 87, 239, 377, 615, 675, 894
AluI AGCT 6 cut(s) 87, 239, 377, 615, 675, 894
Alw26I GTCTC 2 cut(s) 503, 943
AlwI GGATC 3 cut(s) 206, 786, 823
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 5 cut(s) 117, 128, 133, 271, 761
ApaI GGGCCC 1 cut(s) 132
ApeKI GCWGC 4 cut(s) 87, 236, 377, 971
ApoI RAATTY 1 cut(s) 752
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AspLEI GCGC 2 cut(s) 640, 659
AspS9I GGNCC 4 cut(s) 118, 128, 129, 183
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 6 cut(s) 89, 133, 338, 383, 554, 958
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 274
BbvI GCAGC 4 cut(s) 74, 248, 364, 958
BccI CCATC 8 cut(s) 173, 263, 296, 352, 524, 599, 719, 848
BceAI ACGGC 2 cut(s) 65, 258
BciT130I CCWGG 2 cut(s) 116, 492
BciVI GTATCC 1 cut(s) 855
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 503, 943
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 5 cut(s) 26, 602, 633, 759, 975
BfmI CTRYAG 1 cut(s) 592
BfoI RGCGCY 1 cut(s) 660
BfuAI ACCTGC 1 cut(s) 540
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 5 cut(s) 88, 237, 378, 400, 972
BlsI GCNGC 5 cut(s) 89, 238, 379, 401, 973
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 3 cut(s) 116, 132, 492
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 4 cut(s) 118, 128, 129, 183
BmiI GGNNCC 4 cut(s) 130, 184, 584, 906
BmrFI CCNGG 3 cut(s) 116, 132, 492
BmrI ACTGGG 2 cut(s) 115, 910
BmsI GCATC 4 cut(s) 114, 208, 728, 784
BmuI ACTGGG 2 cut(s) 115, 910
BpiI GAAGAC 1 cut(s) 274
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 3 cut(s) 863, 909, 1071
Bsc4I CCNNNNNNNGG 6 cut(s) 115, 126, 165, 790, 854, 869
Bse1I ACTGG 2 cut(s) 121, 905
BseBI CCWGG 2 cut(s) 116, 492
BseDI CCNNGG 3 cut(s) 863, 909, 1071
BseGI GGATG 4 cut(s) 105, 184, 535, 799
BseLI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 790, 854, 869
BseMII CTCAG 2 cut(s) 114, 702
BseNI ACTGG 2 cut(s) 121, 905
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 4 cut(s) 74, 248, 364, 958
BshFI GGCC 5 cut(s) 119, 130, 135, 273, 763
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 790, 854, 869
BsmAI GTCTC 2 cut(s) 503, 943
BsmBI CGTCTC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 5 cut(s) 119, 130, 135, 273, 763
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 4 cut(s) 211, 568, 778, 815
Bsp19I CCATGG 2 cut(s) 863, 1071
BspACI CCGC 2 cut(s) 52, 399
BspANI GGCC 5 cut(s) 119, 130, 135, 273, 763
BspCNI CTCAG 2 cut(s) 113, 701
BspLI GGNNCC 4 cut(s) 130, 184, 584, 906
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 3 cut(s) 206, 786, 823
BspQI GCTCTTC 1 cut(s) 622
BsrI ACTGG 2 cut(s) 121, 905
BssECI CCNNGG 3 cut(s) 863, 909, 1071
BssMI GATC 4 cut(s) 211, 568, 778, 815
BssNI GRCGYC 1 cut(s) 496
BssT1I CCWWGG 3 cut(s) 863, 909, 1071
Bst2UI CCWGG 2 cut(s) 116, 492
Bst4CI ACNGT 3 cut(s) 152, 593, 671
Bst6I CTCTTC 3 cut(s) 63, 201, 622
BstACI GRCGYC 1 cut(s) 496
BstDEI CTNAG 2 cut(s) 100, 688
BstDSI CCRYGG 2 cut(s) 863, 1071
BstENI CCTNNNNNAGG 1 cut(s) 788
BstF5I GGATG 4 cut(s) 105, 184, 535, 799
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 2 cut(s) 640, 659
BstKTI GATC 4 cut(s) 214, 571, 781, 818
BstMAI GTCTC 2 cut(s) 503, 943
BstMBI GATC 4 cut(s) 211, 568, 778, 815
BstNI CCWGG 2 cut(s) 116, 492
BstSCI CCNGG 3 cut(s) 114, 130, 490
BstSFI CTRYAG 1 cut(s) 592
BstSLI GKGCMC 1 cut(s) 132
BstV1I GCAGC 4 cut(s) 74, 248, 364, 958
BstV2I GAAGAC 1 cut(s) 274
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 5 cut(s) 119, 130, 135, 273, 763
BtgI CCRYGG 2 cut(s) 863, 1071
BtsCI GGATG 4 cut(s) 105, 184, 535, 799
BtsIMutI CAGTG 2 cut(s) 589, 973
BveI ACCTGC 1 cut(s) 540
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 2 cut(s) 640, 659
Cfr13I GGNCC 4 cut(s) 118, 128, 129, 183
Csp6I GTAC 2 cut(s) 29, 738
CviAII CATG 7 cut(s) 175, 266, 358, 449, 864, 869, 1072
CviQI GTAC 2 cut(s) 29, 738
DdeI CTNAG 2 cut(s) 100, 688
DpnI GATC 4 cut(s) 213, 570, 780, 817
DpnII GATC 4 cut(s) 211, 568, 778, 815
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 63, 201, 622
EarI CTCTTC 3 cut(s) 63, 201, 622
Eco130I CCWWGG 3 cut(s) 863, 909, 1071
Eco147I AGGCCT 1 cut(s) 763
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 603
EcoNI CCTNNNNNAGG 1 cut(s) 788
EcoRI GAATTC 1 cut(s) 752
EcoRII CCWGG 2 cut(s) 114, 490
EcoT14I CCWWGG 3 cut(s) 863, 909, 1071
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 3 cut(s) 863, 909, 1071
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 7 cut(s) 178, 269, 361, 452, 867, 872, 1075
FatI CATG 7 cut(s) 174, 265, 357, 448, 863, 868, 1071
FauI CCCGC 1 cut(s) 45
Fnu4HI GCNGC 5 cut(s) 88, 237, 378, 400, 972
FokI GGATG 4 cut(s) 92, 191, 542, 806
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 5 cut(s) 88, 237, 378, 400, 972
FspBI CTAG 5 cut(s) 26, 602, 633, 759, 975
FspI TGCGCA 1 cut(s) 639
GlaI GCGC 2 cut(s) 639, 658
GluI GCNGC 5 cut(s) 88, 237, 378, 400, 972
GsuI CTGGAG 1 cut(s) 770
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 5 cut(s) 119, 130, 135, 273, 763
HapII CCGG 1 cut(s) 132
HhaI GCGC 2 cut(s) 640, 659
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 7 cut(s) 178, 269, 361, 452, 867, 872, 1075
Hin6I GCGC 2 cut(s) 638, 657
HinP1I GCGC 2 cut(s) 638, 657
HincII GTYRAC 3 cut(s) 58, 418, 457
HindII GTYRAC 3 cut(s) 58, 418, 457
HinfI GANTC 6 cut(s) 71, 92, 98, 111, 419, 629
HpaII CCGG 1 cut(s) 132
HphI GGTGA 6 cut(s) 89, 133, 338, 383, 554, 958
Hpy166II GTNNAC 4 cut(s) 58, 418, 457, 685
Hpy188I TCNGA 6 cut(s) 97, 110, 187, 724, 732, 1046
Hpy188III TCNNGA 3 cut(s) 209, 215, 876
Hpy8I GTNNAC 4 cut(s) 58, 418, 457, 685
Hpy99I CGWCG 2 cut(s) 152, 164
HpyAV CCTTC 4 cut(s) 163, 404, 727, 1072
HpyCH4III ACNGT 3 cut(s) 152, 593, 671
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 8 cut(s) 265, 380, 426, 441, 466, 485, 719, 836
HpyF3I CTNAG 2 cut(s) 100, 688
HpySE526I ACGT 1 cut(s) 496
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 7 cut(s) 178, 269, 361, 452, 867, 872, 1075
HspAI GCGC 2 cut(s) 638, 657
Kzo9I GATC 4 cut(s) 211, 568, 778, 815
LguI GCTCTTC 1 cut(s) 622
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 4 cut(s) 74, 248, 364, 958
LweI GCATC 4 cut(s) 114, 208, 728, 784
MaeI CTAG 5 cut(s) 26, 602, 633, 759, 975
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 3 cut(s) 152, 542, 896
MalI GATC 4 cut(s) 213, 570, 780, 817
MboI GATC 4 cut(s) 211, 568, 778, 815
MboII GAAGA 6 cut(s) 80, 188, 274, 609, 713, 716
MfeI CAATTG 1 cut(s) 807
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 6 cut(s) 366, 486, 609, 752, 800, 807
MlyI GAGTC 4 cut(s) 80, 92, 101, 413
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 7 cut(s) 51, 155, 263, 365, 533, 794, 940
Mph1103I ATGCAT 1 cut(s) 721
MseI TTAA 1 cut(s) 1084
MslI CAYNNNNRTG 1 cut(s) 1070
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 116, 132, 492
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 1 cut(s) 836
MvaI CCWGG 2 cut(s) 116, 492
NciI CCSGG 1 cut(s) 132
NcoI CCATGG 2 cut(s) 863, 1071
NdeII GATC 4 cut(s) 211, 568, 778, 815
NlaIII CATG 7 cut(s) 178, 269, 361, 452, 867, 872, 1075
NlaIV GGNNCC 4 cut(s) 130, 184, 584, 906
NmuCI GTSAC 3 cut(s) 152, 542, 896
NsbI TGCGCA 1 cut(s) 639
NsiI ATGCAT 1 cut(s) 721
PceI AGGCCT 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 622
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 1 cut(s) 836
PfeI GAWTC 2 cut(s) 111, 629
PflMI CCANNNNNTGG 1 cut(s) 869
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 5 cut(s) 89, 238, 379, 401, 973
PleI GAGTC 4 cut(s) 79, 92, 100, 413
PpsI GAGTC 4 cut(s) 79, 92, 100, 413
Psp6I CCWGG 2 cut(s) 114, 490
PspGI CCWGG 2 cut(s) 114, 490
PspN4I GGNNCC 4 cut(s) 130, 184, 584, 906
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 4 cut(s) 118, 128, 129, 183
PstNI CAGNNNCTG 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 30, 739
RsaNI GTAC 2 cut(s) 29, 738
RseI CAYNNNNRTG 1 cut(s) 1070
SapI GCTCTTC 1 cut(s) 622
SaqAI TTAA 1 cut(s) 1084
SatI GCNGC 5 cut(s) 88, 237, 378, 400, 972
Sau3AI GATC 4 cut(s) 211, 568, 778, 815
Sau96I GGNCC 4 cut(s) 118, 128, 129, 183
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 4 cut(s) 80, 92, 101, 413
ScrFI CCNGG 3 cut(s) 116, 132, 492
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 4 cut(s) 114, 208, 728, 784
SfcI CTRYAG 1 cut(s) 592
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 6 cut(s) 366, 486, 609, 752, 800, 807
SseBI AGGCCT 1 cut(s) 763
SsiI CCGC 2 cut(s) 52, 399
SspMI CTAG 5 cut(s) 26, 602, 633, 759, 975
StuI AGGCCT 1 cut(s) 763
StyD4I CCNGG 3 cut(s) 114, 130, 490
StyI CCWWGG 3 cut(s) 863, 909, 1071
TaaI ACNGT 3 cut(s) 152, 593, 671
TaiI ACGT 1 cut(s) 499
TaqI TCGA 2 cut(s) 7, 147
TasI AATT 6 cut(s) 366, 486, 609, 752, 800, 807
TatI WGTACW 1 cut(s) 28
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 2 cut(s) 111, 629
Tru1I TTAA 1 cut(s) 1084
Tru9I TTAA 1 cut(s) 1084
TscAI CASTG 2 cut(s) 596, 973
TseFI GTSAC 3 cut(s) 152, 542, 896
TseI GCWGC 4 cut(s) 87, 236, 377, 971
Tsp45I GTSAC 3 cut(s) 152, 542, 896
TspDTI ATGAA 1 cut(s) 465
TspRI CASTG 2 cut(s) 596, 973
Van91I CCANNNNNTGG 1 cut(s) 869
VpaK11BI GGWCC 1 cut(s) 183
XagI CCTNNNNNAGG 1 cut(s) 788
XapI RAATTY 1 cut(s) 752
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
XspI CTAG 5 cut(s) 26, 602, 633, 759, 975
ZraI GACGTC 1 cut(s) 497
ZrmI AGTACT 1 cut(s) 30
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.