MD07G1000100.v1.1

Ethylbenzene dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010461
Physical Location & Seq
Reverse (-)
11117 .. 18697
7581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1000100.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGAAGGGTTTTTACAGGCAAAGGAAAAGCAGTAGCGTCGGTGGTACCCCTAAGAAGAAAAAATCCCAATCGGTCGCTCACCCAAAAGCTACTGCTGCCTTTTTCGGCTCAGGTGTAATTTCACACGGCTCTCCGGAGCTCAAAGTGCTTCGATTAGTGTTGGTGGTACTAGTATACTGCGTGCTGCTGATACGACAGGCCAATTCTCACACCGAGTCGGGGGAATGGAGCTGCGAGTCGGAATCTGAGATCCGGGTCCAGGCCGAGTTCGGACCGGGCCTTATCACTCTGGATGGTCATGCCGACGACTGGAAGGATATCGATGGGTTCGAGTTCTCCCTCCTCCCTGCTCTTGACCCAGATGCTGAAAACGAGTACAAAGGTGGAAAGATGACTGTTAAGGCTTTGCATGATGGCCGTGATGCCTTCTTCATGGTTCAAGTTGATGGGGACTACGCTTACTCTAAGGGGGACAACAATAAATGCCCATCTGTTGCCCTCATGTTTCAAATTGGCAGAGATTCAACTTATCATAGCATGGGTGGCTGCAAGGAAGGAGTTGACTCTTGCACAAACAAGACCTGCAAAGGCCATGAAGTTGACATTATGCACTTCTCAATTGGAAATGCTATTCCTGGACGACTCTATGGTGGAAACCCTATAGACAACAGCAATGGAAATGGCGGTGACAGGTTTGGTCATTTGGTTGATCTTTATGCATGGAATCCGCATTGTAGATACCTAGACGGAATTGGTTCTTCAGCAAATGATTCTAGCGCACAAAATGATTGGAAAGGTGCTTGGTGGCACAGTAGCTTGAATGTTCACTCAGGTTTTGTGGAAGAAGATAGCCCGTATTCATCAGATGCTCAGAAGGGCACATTTTATTTTGAATTCTCAAGGCCTCTGAGAACTATGGATCGTCTCCAACAGGACGTTCAATTCACAATTGGAGGATCAAGTAAGATGTCAGTTGCATTCTGGTATCCTGTAGATAAAAAACCGTGGCATGGCTCTGGACACTATTCTATTAGCTGTGACTGGGTTCCCTTAGATATCTCTTCAAGAAGTTCTCTGCTGACCACAGCAAGACCAAGTAGCTCGGTTAATGCTGCCAGTGTTTTTGCCCTTCTCTTATCAGTAATCTCGTTGTGTGCTTCTGTCTTTATTGGGTATTGGGTCGTGAAACCCAAAACTTTTCAGTTAACTCCCATGGAAAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EB_dh

Protein Analysis

409

Amino Acids

44.82

Weight (kDa)

6.27

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EB_dh PF09459 102 - 343 4.5e-40 Ethylbenzene dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 590
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 174
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 2 cut(s) 684, 728
AclWI GGATC 3 cut(s) 244, 927, 964
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 1 cut(s) 893
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 3 cut(s) 46, 168, 377
AfiI CCNNNNNNNGG 4 cut(s) 219, 259, 309, 1010
AgsI TTSAA 7 cut(s) 440, 509, 525, 820, 893, 941, 1065
AhlI ACTAGT 1 cut(s) 169
AjnI CCWGG 2 cut(s) 258, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
AluBI AGCT 6 cut(s) 89, 139, 231, 816, 1035, 1101
AluI AGCT 6 cut(s) 89, 139, 231, 816, 1035, 1101
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 929
AlwI GGATC 3 cut(s) 244, 927, 964
AlwNI CAGNNNCTG 1 cut(s) 365
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 6 cut(s) 198, 261, 277, 415, 589, 902
ApeKI GCWGC 5 cut(s) 95, 184, 231, 546, 1112
ApoI RAATTY 1 cut(s) 893
Asp700I GAANNNNTTC 1 cut(s) 754
Asp718I GGTACC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 779
AspS9I GGNCC 3 cut(s) 256, 272, 277
AsuC2I CCSGG 2 cut(s) 254, 276
AsuHPI GGTGA 2 cut(s) 71, 698
AvaII GGWCC 2 cut(s) 256, 272
BaeGI GKGCMC 1 cut(s) 881
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 141
BarI GAAGNNNNNNTAC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 5 cut(s) 82, 171, 218, 533, 1099
BccI CCATC 5 cut(s) 287, 317, 407, 440, 496
BceAI ACGGC 2 cut(s) 142, 402
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 2 cut(s) 260, 636
BciVI GTATCC 1 cut(s) 996
BcnI CCSGG 2 cut(s) 254, 276
BcoDI GTCTC 1 cut(s) 929
BcuI ACTAGT 1 cut(s) 169
BfaI CTAG 3 cut(s) 170, 743, 774
BfmI CTRYAG 2 cut(s) 660, 990
BfuAI ACCTGC 1 cut(s) 590
BfuI GTATCC 1 cut(s) 996
BisI GCNGC 5 cut(s) 96, 185, 232, 547, 1113
BlsI GCNGC 5 cut(s) 97, 186, 233, 548, 1114
Bme1390I CCNGG 4 cut(s) 254, 260, 276, 636
Bme18I GGWCC 2 cut(s) 256, 272
BmgT120I GGNCC 3 cut(s) 256, 272, 277
BmiI GGNNCC 3 cut(s) 46, 257, 1047
BmrFI CCNGG 4 cut(s) 254, 260, 276, 636
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 3 cut(s) 352, 412, 856
BmuI ACTGGG 1 cut(s) 1051
Bpu10I CCTNAGC 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 883
BpuMI CCSGG 2 cut(s) 254, 276
Bsa29I ATCGAT 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 1004, 1212
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 259, 309, 1010
Bse1I ACTGG 3 cut(s) 314, 1046, 1116
Bse3DI GCAATG 1 cut(s) 679
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 2 cut(s) 260, 636
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 1004, 1212
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 4 cut(s) 219, 259, 309, 1010
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 5 cut(s) 123, 237, 843, 884, 899
BseNI ACTGG 3 cut(s) 314, 1046, 1116
BseRI GAGGAG 1 cut(s) 332
BseSI GKGCMC 1 cut(s) 881
BseXI GCAGC 5 cut(s) 82, 171, 218, 533, 1099
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 6 cut(s) 200, 263, 279, 417, 591, 904
BshNI GGYRCC 1 cut(s) 44
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 3 cut(s) 134, 253, 275
BslFI GGGAC 2 cut(s) 464, 485
BslI CCNNNNNNNGG 4 cut(s) 219, 259, 309, 1010
BsmAI GTCTC 1 cut(s) 929
BsmBI CGTCTC 1 cut(s) 929
BsmFI GGGAC 2 cut(s) 464, 485
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 6 cut(s) 200, 263, 279, 417, 591, 904
Bsp1286I GDGCHC 2 cut(s) 141, 881
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 4 cut(s) 249, 709, 919, 956
Bsp19I CCATGG 1 cut(s) 1212
BspACI CCGC 2 cut(s) 684, 728
BspANI GGCC 6 cut(s) 200, 263, 279, 417, 591, 904
BspCNI CTCAG 5 cut(s) 122, 238, 842, 883, 900
BspDI ATCGAT 1 cut(s) 321
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 3 cut(s) 46, 257, 1047
BspMI ACCTGC 1 cut(s) 590
BspPI GGATC 3 cut(s) 244, 927, 964
BspT107I GGYRCC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 679
BsrI ACTGG 3 cut(s) 314, 1046, 1116
BssECI CCNNGG 2 cut(s) 1004, 1212
BssMI GATC 4 cut(s) 249, 709, 919, 956
BssNAI GTATAC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 1212
Bst1107I GTATAC 1 cut(s) 175
Bst2UI CCWGG 2 cut(s) 260, 636
Bst4CI ACNGT 3 cut(s) 397, 812, 1005
Bst6I CTCTTC 1 cut(s) 1066
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 8 cut(s) 51, 109, 246, 465, 829, 870, 908, 1051
BstDSI CCRYGG 2 cut(s) 1004, 1212
BstF5I GGATG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 779
BstKTI GATC 4 cut(s) 252, 712, 922, 959
BstMAI GTCTC 1 cut(s) 929
BstMBI GATC 4 cut(s) 249, 709, 919, 956
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 3 cut(s) 95, 145, 543
BstNI CCWGG 2 cut(s) 260, 636
BstSCI CCNGG 4 cut(s) 252, 258, 274, 634
BstSFI CTRYAG 2 cut(s) 660, 990
BstSLI GKGCMC 1 cut(s) 881
BstV1I GCAGC 5 cut(s) 82, 171, 218, 533, 1099
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BstZ17I GTATAC 1 cut(s) 175
Bsu15I ATCGAT 1 cut(s) 321
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 6 cut(s) 200, 263, 279, 417, 591, 904
BsuTUI ATCGAT 1 cut(s) 321
BtgI CCRYGG 2 cut(s) 1004, 1212
BtsCI GGATG 1 cut(s) 298
BtsIMutI CAGTG 1 cut(s) 1123
BveI ACCTGC 1 cut(s) 590
Cac8I GCNNGC 1 cut(s) 182
CaiI CAGNNNCTG 1 cut(s) 365
CfoI GCGC 1 cut(s) 779
Cfr13I GGNCC 3 cut(s) 256, 272, 277
ClaI ATCGAT 1 cut(s) 321
CpoI CGGWCCG 1 cut(s) 272
CseI GACGC 1 cut(s) 25
Csp6I GTAC 3 cut(s) 45, 167, 376
CspI CGGWCCG 1 cut(s) 272
CviAII CATG 9 cut(s) 299, 410, 433, 502, 538, 593, 720, 1010, 1213
CviQI GTAC 3 cut(s) 45, 167, 376
DdeI CTNAG 8 cut(s) 51, 109, 246, 465, 829, 870, 908, 1051
DpnI GATC 4 cut(s) 251, 711, 921, 958
DpnII GATC 4 cut(s) 249, 709, 919, 956
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 1066
EarI CTCTTC 1 cut(s) 1066
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 1212
Eco147I AGGCCT 1 cut(s) 904
Eco24I GRGCYC 1 cut(s) 141
Eco32I GATATC 2 cut(s) 319, 1057
Eco47I GGWCC 2 cut(s) 256, 272
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 744
EcoICRI GAGCTC 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 893
EcoRII CCWGG 2 cut(s) 258, 634
EcoRV GATATC 2 cut(s) 319, 1057
EcoT14I CCWWGG 1 cut(s) 1212
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 1212
Esp3I CGTCTC 1 cut(s) 929
FaeI CATG 9 cut(s) 302, 413, 436, 505, 541, 596, 723, 1013, 1216
FaqI GGGAC 2 cut(s) 464, 485
FatI CATG 9 cut(s) 298, 409, 432, 501, 537, 592, 719, 1009, 1212
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 5 cut(s) 96, 185, 232, 547, 1113
FokI GGATG 1 cut(s) 305
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 5 cut(s) 96, 185, 232, 547, 1113
FspBI CTAG 3 cut(s) 170, 743, 774
GlaI GCGC 1 cut(s) 778
GluI GCNGC 5 cut(s) 96, 185, 232, 547, 1113
HaeIII GGCC 6 cut(s) 200, 263, 279, 417, 591, 904
HapII CCGG 3 cut(s) 134, 253, 275
HgaI GACGC 1 cut(s) 25
HhaI GCGC 1 cut(s) 779
Hin1II CATG 9 cut(s) 302, 413, 436, 505, 541, 596, 723, 1013, 1216
Hin6I GCGC 1 cut(s) 777
HinP1I GCGC 1 cut(s) 777
HincII GTYRAC 3 cut(s) 562, 601, 1206
HindII GTYRAC 3 cut(s) 562, 601, 1206
HinfI GANTC 8 cut(s) 215, 236, 242, 521, 563, 642, 724, 770
HpaI GTTAAC 1 cut(s) 1206
HpaII CCGG 3 cut(s) 134, 253, 275
HphI GGTGA 2 cut(s) 71, 698
Hpy166II GTNNAC 5 cut(s) 175, 562, 601, 826, 1206
Hpy188I TCNGA 6 cut(s) 241, 247, 272, 865, 873, 909
Hpy188III TCNNGA 6 cut(s) 134, 290, 353, 1017, 1065, 1183
Hpy8I GTNNAC 5 cut(s) 175, 562, 601, 826, 1206
Hpy99I CGWCG 2 cut(s) 41, 308
HpyAV CCTTC 5 cut(s) 307, 436, 548, 868, 1139
HpyCH4III ACNGT 3 cut(s) 397, 812, 1005
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 7 cut(s) 409, 549, 570, 585, 610, 719, 977
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 145, 543
HpyF3I CTNAG 8 cut(s) 51, 109, 246, 465, 829, 870, 908, 1051
HpySE526I ACGT 1 cut(s) 936
Hsp92II CATG 9 cut(s) 302, 413, 436, 505, 541, 596, 723, 1013, 1216
HspAI GCGC 1 cut(s) 777
Kpn2I TCCGGA 1 cut(s) 133
KpnI GGTACC 1 cut(s) 48
KspAI GTTAAC 1 cut(s) 1206
Kzo9I GATC 4 cut(s) 249, 709, 919, 956
LmnI GCTCC 2 cut(s) 136, 228
Lsp1109I GCAGC 5 cut(s) 82, 171, 218, 533, 1099
LweI GCATC 3 cut(s) 352, 412, 856
MaeI CTAG 3 cut(s) 170, 743, 774
MaeII ACGT 1 cut(s) 936
MaeIII GTNAC 2 cut(s) 686, 1037
MalI GATC 4 cut(s) 251, 711, 921, 958
MboI GATC 4 cut(s) 249, 709, 919, 956
MboII GAAGA 6 cut(s) 67, 421, 750, 854, 857, 1053
MfeI CAATTG 2 cut(s) 618, 948
MflI RGATCY 1 cut(s) 249
MhlI GDGCHC 2 cut(s) 141, 881
MluCI AATT 8 cut(s) 117, 202, 510, 618, 750, 893, 941, 948
MlyI GAGTC 4 cut(s) 224, 245, 557, 636
MmeI TCCRAC 2 cut(s) 219, 952
MnlI CCTC 5 cut(s) 350, 353, 509, 915, 947
Mph1103I ATGCAT 1 cut(s) 721
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 754
MseI TTAA 4 cut(s) 399, 1107, 1205, 1225
MspI CCGG 3 cut(s) 134, 253, 275
MspR9I CCNGG 4 cut(s) 254, 260, 276, 636
MunI CAATTG 2 cut(s) 618, 948
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 2 cut(s) 260, 636
MwoI GCNNNNNNNGC 3 cut(s) 95, 145, 543
NciI CCSGG 2 cut(s) 254, 276
NcoI CCATGG 1 cut(s) 1212
NdeII GATC 4 cut(s) 249, 709, 919, 956
NlaIII CATG 9 cut(s) 302, 413, 436, 505, 541, 596, 723, 1013, 1216
NlaIV GGNNCC 3 cut(s) 46, 257, 1047
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 2 cut(s) 686, 1037
NsiI ATGCAT 1 cut(s) 721
PceI AGGCCT 1 cut(s) 904
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PctI GAATGC 1 cut(s) 977
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 4 cut(s) 242, 521, 724, 770
PfoI TCCNGGA 1 cut(s) 634
PkrI GCNGC 5 cut(s) 97, 186, 233, 548, 1114
PleI GAGTC 4 cut(s) 223, 244, 557, 636
PpsI GAGTC 4 cut(s) 223, 244, 557, 636
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 258, 634
PspGI CCWGG 2 cut(s) 258, 634
PspN4I GGNNCC 3 cut(s) 46, 257, 1047
PspPI GGNCC 3 cut(s) 256, 272, 277
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 249
RsaI GTAC 3 cut(s) 46, 168, 377
RsaNI GTAC 3 cut(s) 45, 167, 376
Rsr2I CGGWCCG 1 cut(s) 272
RsrII CGGWCCG 1 cut(s) 272
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 4 cut(s) 399, 1107, 1205, 1225
SatI GCNGC 5 cut(s) 96, 185, 232, 547, 1113
Sau3AI GATC 4 cut(s) 249, 709, 919, 956
Sau96I GGNCC 3 cut(s) 256, 272, 277
SchI GAGTC 4 cut(s) 224, 245, 557, 636
ScrFI CCNGG 4 cut(s) 254, 260, 276, 636
SduI GDGCHC 2 cut(s) 141, 881
SfaNI GCATC 3 cut(s) 352, 412, 856
SfcI CTRYAG 2 cut(s) 660, 990
SinI GGWCC 2 cut(s) 256, 272
SmlI CTYRAG 1 cut(s) 898
SmoI CTYRAG 1 cut(s) 898
SpeI ACTAGT 1 cut(s) 169
Sse9I AATT 8 cut(s) 117, 202, 510, 618, 750, 893, 941, 948
SseBI AGGCCT 1 cut(s) 904
SsiI CCGC 2 cut(s) 684, 728
SspMI CTAG 3 cut(s) 170, 743, 774
SstI GAGCTC 1 cut(s) 141
StuI AGGCCT 1 cut(s) 904
StyD4I CCNGG 4 cut(s) 252, 258, 274, 634
StyI CCWWGG 1 cut(s) 1212
TaaI ACNGT 3 cut(s) 397, 812, 1005
TaiI ACGT 1 cut(s) 939
TaqI TCGA 3 cut(s) 151, 321, 330
TaqII GACCGA 1 cut(s) 61
TasI AATT 8 cut(s) 117, 202, 510, 618, 750, 893, 941, 948
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 4 cut(s) 242, 521, 724, 770
Tru1I TTAA 4 cut(s) 399, 1107, 1205, 1225
Tru9I TTAA 4 cut(s) 399, 1107, 1205, 1225
TscAI CASTG 1 cut(s) 1123
TseFI GTSAC 2 cut(s) 686, 1037
TseI GCWGC 5 cut(s) 95, 184, 231, 546, 1112
Tsp45I GTSAC 2 cut(s) 686, 1037
TspDTI ATGAA 4 cut(s) 17, 421, 609, 849
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 1 cut(s) 1123
VpaK11BI GGWCC 2 cut(s) 256, 272
XapI RAATTY 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 3 cut(s) 170, 743, 774
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.