Rh6AG001000

Ethylbenzene dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010461
Physical Location & Seq
Reverse (-)
266435 .. 272063
5629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG001000.1

Sequence Viewer

Length: 822 bp
ATGCTTCGACCATGGCTACTGCTGTTAGTATTTTGTGTGCCTCTGCTACAACGGGTCAACTCGCACGAAGAGTCGGGTGAATGGAGCTGCGAGTCCGACTCAGACATCCGAATCCAGGCCCAGTTCGGGCCCGGCCTTATCACCCTCGATGGTCACGCCGACGATTGGAAGGACACGGATGGGTCCGAGTTCTCTCTTCTCCCTGCTCTTGACCCAGATGCTGAAACTGAATACACAGGTGGAAAGATGACCGTCAAGGCTTTGCATGATGGCCGTGATGTCTTCTTTATGGTTCAAGTTGATGGGGATTACGCTTACTCAAAAGGTGAGAATACCAAATGCCCATCTGTTGCCCTCATGTTTCAAATTGGCGAAGCTGCAACTTATCATAATATGGGCGGCTGTAAGGAAGGAGTTGACTCTTGCACAAACAAGACTTGCAAATCCCATGAAGTTGACATTATGCATTTCTCTATTGGAAATGCAATTCCTGGACGTCTCTATGGTGGTAACCCAGTGGACAACAGGGATGGAAATGGAGGTGACAGGTTTGGTCATTTGAATGATCTTTATGGTTGGAACCCACACTGTAGATACCTAGATGGAATTAGCTCTTCAGCAAATGATTCTAGTGCACAAAATGATTGGAAAGGTGCTTGGTGGCACAGTAGCTTGAATGTTCACTCAGGTTTTGTGGAAGAAGATGGTCCTTATGCATCAGATGGTCAAAAGGGTACATACTATTTTGAATTCTCTAGGCCTTTGAGAACTATGGATCGCCTCCAACAGGTCAGCCTCCAAATTTTGAGCTACCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EB_dh

Protein Analysis

273

Amino Acids

30.37

Weight (kDa)

4.77

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EB_dh PF09459 54 - 267 1.8e-31 Ethylbenzene dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 499
AciI CCGC 1 cut(s) 399
AclWI GGATC 1 cut(s) 783
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 3 cut(s) 749, 801, 816
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 1 cut(s) 736
AfiI CCNNNNNNNGG 4 cut(s) 115, 126, 165, 787
AgsI TTSAA 5 cut(s) 296, 365, 562, 676, 749
AjnI CCWGG 2 cut(s) 114, 490
AluBI AGCT 5 cut(s) 87, 377, 612, 672, 810
AluI AGCT 5 cut(s) 87, 377, 612, 672, 810
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 1 cut(s) 503
Alw44I GTGCAC 1 cut(s) 633
AlwI GGATC 1 cut(s) 783
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 5 cut(s) 117, 128, 133, 271, 758
ApaI GGGCCC 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 2 cut(s) 87, 377
ApoI RAATTY 3 cut(s) 749, 801, 816
AspS9I GGNCC 5 cut(s) 118, 128, 129, 183, 707
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 4 cut(s) 89, 133, 338, 554
AvaII GGWCC 2 cut(s) 183, 707
BaeGI GKGCMC 2 cut(s) 132, 637
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 2 cut(s) 74, 364
BccI CCATC 9 cut(s) 143, 173, 263, 296, 352, 524, 596, 698, 716
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 2 cut(s) 116, 492
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 503
BfaI CTAG 3 cut(s) 599, 630, 756
BfmI CTRYAG 1 cut(s) 589
BisI GCNGC 3 cut(s) 88, 378, 400
BlsI GCNGC 3 cut(s) 89, 379, 401
Bme1390I CCNGG 3 cut(s) 116, 132, 492
Bme18I GGWCC 2 cut(s) 183, 707
BmgT120I GGNCC 5 cut(s) 118, 128, 129, 183, 707
BmiI GGNNCC 3 cut(s) 130, 184, 581
BmrFI CCNGG 3 cut(s) 116, 132, 492
BmrI ACTGGG 2 cut(s) 115, 509
BmsI GCATC 2 cut(s) 208, 725
BmuI ACTGGG 2 cut(s) 115, 509
BpiI GAAGAC 1 cut(s) 274
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpuMI CCSGG 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 126, 165, 787
Bse1I ACTGG 2 cut(s) 121, 515
BseBI CCWGG 2 cut(s) 116, 492
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 3 cut(s) 105, 184, 535
BseLI CCNNNNNNNGG 4 cut(s) 115, 126, 165, 787
BseMII CTCAG 2 cut(s) 114, 699
BseNI ACTGG 2 cut(s) 121, 515
BseSI GKGCMC 2 cut(s) 132, 637
BseXI GCAGC 2 cut(s) 74, 364
BshFI GGCC 5 cut(s) 119, 130, 135, 273, 760
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 4 cut(s) 115, 126, 165, 787
BsmAI GTCTC 1 cut(s) 503
BsmBI CGTCTC 1 cut(s) 503
BsnI GGCC 5 cut(s) 119, 130, 135, 273, 760
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 132, 637
Bsp143I GATC 2 cut(s) 565, 775
Bsp19I CCATGG 1 cut(s) 11
BspACI CCGC 1 cut(s) 399
BspANI GGCC 5 cut(s) 119, 130, 135, 273, 760
BspCNI CTCAG 2 cut(s) 113, 698
BspLI GGNNCC 3 cut(s) 130, 184, 581
BspPI GGATC 1 cut(s) 783
BspQI GCTCTTC 1 cut(s) 619
BsrI ACTGG 2 cut(s) 121, 515
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 2 cut(s) 565, 775
BssNI GRCGYC 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 11
Bst2UI CCWGG 2 cut(s) 116, 492
Bst4CI ACNGT 3 cut(s) 253, 590, 668
Bst6I CTCTTC 3 cut(s) 63, 201, 619
BstACI GRCGYC 1 cut(s) 496
BstDEI CTNAG 2 cut(s) 100, 685
BstDSI CCRYGG 1 cut(s) 11
BstEII GGTNACC 1 cut(s) 509
BstENI CCTNNNNNAGG 1 cut(s) 785
BstF5I GGATG 3 cut(s) 105, 184, 535
BstKTI GATC 2 cut(s) 568, 778
BstMAI GTCTC 1 cut(s) 503
BstMBI GATC 2 cut(s) 565, 775
BstNI CCWGG 2 cut(s) 116, 492
BstPI GGTNACC 1 cut(s) 509
BstSCI CCNGG 3 cut(s) 114, 130, 490
BstSFI CTRYAG 1 cut(s) 589
BstSLI GKGCMC 2 cut(s) 132, 637
BstV1I GCAGC 2 cut(s) 74, 364
BstV2I GAAGAC 1 cut(s) 274
BsuRI GGCC 5 cut(s) 119, 130, 135, 273, 760
BtgI CCRYGG 1 cut(s) 11
BtsCI GGATG 3 cut(s) 105, 184, 535
BtsIMutI CAGTG 2 cut(s) 522, 586
CaiI CAGNNNCTG 1 cut(s) 221
Cfr13I GGNCC 5 cut(s) 118, 128, 129, 183, 707
Csp6I GTAC 1 cut(s) 735
CviAII CATG 4 cut(s) 12, 266, 358, 449
CviQI GTAC 1 cut(s) 735
DdeI CTNAG 2 cut(s) 100, 685
DpnI GATC 2 cut(s) 567, 777
DpnII GATC 2 cut(s) 565, 775
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 63, 201, 619
EarI CTCTTC 3 cut(s) 63, 201, 619
Eco130I CCWWGG 1 cut(s) 11
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 183, 707
Eco57I CTGAAG 1 cut(s) 600
Eco91I GGTNACC 1 cut(s) 509
EcoNI CCTNNNNNAGG 1 cut(s) 785
EcoO65I GGTNACC 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 749
EcoRII CCWGG 2 cut(s) 114, 490
EcoT14I CCWWGG 1 cut(s) 11
EcoT22I ATGCAT 2 cut(s) 468, 718
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 11
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 4 cut(s) 15, 269, 361, 452
FatI CATG 4 cut(s) 11, 265, 357, 448
Fnu4HI GCNGC 3 cut(s) 88, 378, 400
FokI GGATG 3 cut(s) 92, 191, 542
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 3 cut(s) 88, 378, 400
FspBI CTAG 3 cut(s) 599, 630, 756
GluI GCNGC 3 cut(s) 88, 378, 400
HaeIII GGCC 5 cut(s) 119, 130, 135, 273, 760
HapII CCGG 1 cut(s) 132
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 4 cut(s) 15, 269, 361, 452
HincII GTYRAC 3 cut(s) 58, 418, 457
HindII GTYRAC 3 cut(s) 58, 418, 457
HinfI GANTC 6 cut(s) 71, 92, 98, 111, 419, 626
HpaII CCGG 1 cut(s) 132
HphI GGTGA 4 cut(s) 89, 133, 338, 554
Hpy166II GTNNAC 6 cut(s) 58, 418, 457, 520, 635, 682
Hpy188I TCNGA 5 cut(s) 97, 103, 110, 187, 721
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 6 cut(s) 58, 418, 457, 520, 635, 682
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 163, 404
HpyCH4III ACNGT 3 cut(s) 253, 590, 668
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 8 cut(s) 265, 380, 426, 441, 466, 485, 635, 716
HpyF3I CTNAG 2 cut(s) 100, 685
HpySE526I ACGT 1 cut(s) 496
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 4 cut(s) 15, 269, 361, 452
Kzo9I GATC 2 cut(s) 565, 775
LguI GCTCTTC 1 cut(s) 619
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 2 cut(s) 74, 364
LweI GCATC 2 cut(s) 208, 725
MaeI CTAG 3 cut(s) 599, 630, 756
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 3 cut(s) 152, 509, 542
MalI GATC 2 cut(s) 567, 777
MboI GATC 2 cut(s) 565, 775
MboII GAAGA 6 cut(s) 80, 188, 274, 606, 710, 713
MhlI GDGCHC 2 cut(s) 132, 637
MluCI AATT 6 cut(s) 366, 486, 606, 749, 801, 816
MlyI GAGTC 4 cut(s) 80, 92, 101, 413
MmeI TCCRAC 3 cut(s) 120, 557, 808
MnlI CCTC 6 cut(s) 51, 155, 365, 533, 791, 806
Mph1103I ATGCAT 2 cut(s) 468, 718
MslI CAYNNNNRTG 1 cut(s) 561
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 116, 132, 492
MvaI CCWGG 2 cut(s) 116, 492
NciI CCSGG 1 cut(s) 132
NcoI CCATGG 1 cut(s) 11
NdeII GATC 2 cut(s) 565, 775
NlaIII CATG 4 cut(s) 15, 269, 361, 452
NlaIV GGNNCC 3 cut(s) 130, 184, 581
NmuCI GTSAC 2 cut(s) 152, 542
NsiI ATGCAT 2 cut(s) 468, 718
PceI AGGCCT 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 619
PfeI GAWTC 2 cut(s) 111, 626
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 3 cut(s) 89, 379, 401
PleI GAGTC 4 cut(s) 79, 92, 100, 413
PpsI GAGTC 4 cut(s) 79, 92, 100, 413
Psp6I CCWGG 2 cut(s) 114, 490
PspEI GGTNACC 1 cut(s) 509
PspGI CCWGG 2 cut(s) 114, 490
PspN4I GGNNCC 3 cut(s) 130, 184, 581
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 5 cut(s) 118, 128, 129, 183, 707
PstNI CAGNNNCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 736
RsaNI GTAC 1 cut(s) 735
RseI CAYNNNNRTG 1 cut(s) 561
SapI GCTCTTC 1 cut(s) 619
SatI GCNGC 3 cut(s) 88, 378, 400
Sau3AI GATC 2 cut(s) 565, 775
Sau96I GGNCC 5 cut(s) 118, 128, 129, 183, 707
SchI GAGTC 4 cut(s) 80, 92, 101, 413
ScrFI CCNGG 3 cut(s) 116, 132, 492
SduI GDGCHC 2 cut(s) 132, 637
SfaNI GCATC 2 cut(s) 208, 725
SfcI CTRYAG 1 cut(s) 589
SinI GGWCC 2 cut(s) 183, 707
SmiMI CAYNNNNRTG 1 cut(s) 561
Sse9I AATT 6 cut(s) 366, 486, 606, 749, 801, 816
SseBI AGGCCT 1 cut(s) 760
SsiI CCGC 1 cut(s) 399
SspMI CTAG 3 cut(s) 599, 630, 756
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 3 cut(s) 114, 130, 490
StyI CCWWGG 1 cut(s) 11
TaaI ACNGT 3 cut(s) 253, 590, 668
TaiI ACGT 1 cut(s) 499
TaqI TCGA 2 cut(s) 7, 147
TasI AATT 6 cut(s) 366, 486, 606, 749, 801, 816
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 2 cut(s) 111, 626
TscAI CASTG 2 cut(s) 522, 593
TseFI GTSAC 2 cut(s) 152, 542
TseI GCWGC 2 cut(s) 87, 377
Tsp45I GTSAC 2 cut(s) 152, 542
TspDTI ATGAA 1 cut(s) 465
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 2 cut(s) 522, 593
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 2 cut(s) 183, 707
XagI CCTNNNNNAGG 1 cut(s) 785
XapI RAATTY 3 cut(s) 749, 801, 816
XspI CTAG 3 cut(s) 599, 630, 756
ZraI GACGTC 1 cut(s) 497
Zsp2I ATGCAT 2 cut(s) 468, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.