AT3G63460

Protein transport protein SEC31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010028
Physical Location & Seq
Reverse (-)
23430623 .. 23437374
6752 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G63460.3

Sequence Viewer

Length: 3315 bp
ATGGCTTGCATCAAGGGGGTGGGCCGATCCGCCTCCGTTGCCCTGGCTCCCGATGCTCCTTACATGGCCGCCGGAACCATGGCCGGAGCCGTCGATCTCTCCTTTAGCTCTTCCGCCAATCTCGAGATCTTTAAGCTCGACTTTCAGTCTGACGATCGCGACTTGCCTCTCGTCGGGGAAATCCCCAGCTCCGAGCGGTTCAATAGACTCGCCTGGGGAAGAAACGGATCTGGATCCGAGGAATTCGCCCTTGGTCTTATTGCCGGCGGCCTTGTCGACGGAAATATAGATCTATGGAATCCTCTTTCTTTGATCGGTTCCCAGCCCAGCGAAAATGCACTCGTTGGCCATCTTTCAGTTCACAAAGGCCCTGTTCGTGGTCTTGAGTTTAATGCTATCAGCTCAAATCTACTTGCTTCTGGGGCTGATGATGGTGAAATTTGCATTTGGGACTTATTAAAGCCTTCAGAGCCTTCTCATTTTCCACTGCTCAAGGGTAGTGGTTCTGCTACGCAAGGTGAAATCTCCTTTATATCGTGGAATAGAAAAGTTCAACAGATATTAGCTTCTACCTCATACAATGGGACTACTGTAATTTGGGACTTGAGGAAGCAGAAGCCTATAATTAACTTTGCAGATTCAGTTCGACGCCGGTGCTCTGTTTTACAGTGGAACCCTAACGTTACTACTCAGATAATGGTTGCATCAGATGACGACAGTTCACCTACTTTGAAGCTCTGGGACATGAGGAATATAATGTCACCTGTGCGCGAGTTTACTGGACACCAAAGAGGGGTAATTGCAATGGAGTGGTGCCCAAGTGACAGCTCATATCTTCTCACCTGTGCTAAGGACAACCGAACGATTTGCTGGGATACAAATACAGCAGAGATTGTGGCTGAGTTACCTGCTGGCAACAATTGGAATTTTGATGTTCATTGGTACCCAAAGATACCTGGAGTTATATCAGCATCTTCATTTGATGGGAAAATTGGCATCTACAATATCGAGGGTTGCAGTCGCTATGGTGTTGAAGAGAATAATTTTGGTACTGCTCCTTTAAAAGCACCAAAATGGTATAAGCGCCCGGTTGGTGCATCTTTTGGATTTGGGGGAAAGCTTGTATCGTGTCACGCAAGGGCCCCTGCAAAGGGTACTTCGAGCATTCTATCCGAGGTTTTTTTGCACAGCCTGGTAACAGAACAGAGTTTGGTGAGTCGAACTTCTGAATTTGAAGCTGCAATAGAAAATGGAGATATGACTTCTCTAAGGGGTTTGTGCGAGAAGAAATCTGAAGAGACTGAATCCGAAGAGGAGAAAGAGACATGGGGCTTGCTGAAAATCATGTTCGAAGAGGAAGGAACTTCAAGGACAAAGTTGATCAGCCATCTTGGCTTTACTTTGCCTGTTGCGGAAAAGGATCAGGCAGTAGATGGGCTCTCATCAGATCTCAATGGCATTAGATTAGAGGATACTGCGGCAGATGCACTGGATCTTGACGACAGTAACGAGGCAGCTGCATTTGCGATGGATAATGGAGAAGACTTCTTTAACAACTTTCCTGCTAAACCGGATACACCTGTATCTACATCTGCCAAAGATTTTATGCCTTCTGACACAGATTTTTCTACCAAAGGAGAAGAAACGCAAGAAATGCAAGAAGAAGAGGAAGAAAGTTCTGACCCAGTTTTTGATAATGCCATCCAGCGAGCGTTGATTGTTGGAGATTACAAGGAGGCGGTGGATCAGTGTATAACTGCAAATAAGATGGCCGATGCTTTAGTTATTGCTCATGTTGGTGGTACAGCGTTGTGGGAGAGTACTCGTGAGAAATATTTGAAGACGAGCAGTGCGCCATACATGAAGGTTGTTTCTGCGATGGTGAACAATGATCTCAGGAGCCTTATCTATACAAGGTCACATAAGTTCTGGAAAGAGACTCTTGCTCTCCTCTGTACTTTTGCACAAGGAGAACAATGGACAACCCTGTGTGATGCCCTTGCCTCGAAGTTGATGGCTGCTGGTAACACTTTGGCTGCAGTTCTCTGCTACATTTGCGCAGGCAATGTTGACAGAACAGTAGAAATTTGGTCGAGGAGCCTTGCAAATGAGCGGGATGGAAGATCTTATGCTGAGCTTCTTCAAGATCTTATGGAGAAGACTCTTGTCCTTGCTTTGGCAACTGGCAACAAAAAGTTCAGCGCATCTCTGTGTAAACTCTTTGAGAGTTATGCTGAGATACTGGCCAGCCAAGGGCTTCTTACAACGGCAATGAAGTACTTGAAAGTTCTGGATTCTGGTGGCTTGTCACCTGAACTTTCAATATTACGTGATCGTATTTCTCTATCTGCAGAACCTGAGACTAACACTACAGCTTCAGGAAACACTCAGCCTCAAAGCACCATGCCATATAATCAGGAGCCAACTCAGGCGCAACCAAACGTTCTTGCTAACCCATATGATAATCAGTATCAGCAACCGTACACTGATTCTTATTATGTCCCTCAAGTTTCACATCCACCCATGCAGCAACCAACCATGTTTATGCCACACCAAGCTCAGCCAGCTCCGCAGCCATCTTTTACTCCAGCTCCTACAAGCAATGCTCAGCCATCCATGAGAACTACATTTGTTCCTTCAACTCCCCCTGCACTGAAGAATGCAGATCAATATCAGCAGCCAACCATGAGTTCTCATTCATTCACGGGACCATCTAACAATGCATACCCTGTTCCCCCGGGTCCTGGTCAATATGCACCTTCTGGCCCTTCACAACTTGGGCAATATCCTAACCCTAAGATGCCCCAAGTTGTTGCTCCAGCAGCTGGACCCATAGGATTTACGCCCATGGCAACTCCAGGAGTTGCTCCAAGATCTGTGCAACCAGCAAGTCCTCCAACACAGCAGGCAGCTGCACAGGCAGCCCCTGCGCCTGCAACTCCGCCACCAACTGTTCAGACTGCAGATACTTCCAACGTTCCAGCCCACCAGAAACCTGTGATAGCAACGTTGACAAGGCTTTTCAATGAGACATCGGAAGCACTGGGAGGCGCACGTGCGAATACTACTAAGAAGCGTGAGATAGAAGACAACTCGAGAAAATTAGGTGCTCTGTTTGTGAAACTCAACAGCGGAGACATCTCCAAGAATGCTGCGGACAAACTCGCACAGCTATGCCAAGCTCTGGACAACAATGACTTCAGCACAGCCCTTCAAATACAGGTACTTCTGACTACCAGCGAATGGGACGAATGCAACTTCTGGCTGGCAACACTAAAGCGGATGATGGTCAAGGCCAGGCAAAATGTGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SRA1 Sec16_C WD40

Protein Analysis

1104

Amino Acids

119.87

Weight (kDa)

5.0

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 86 - 238 1.8e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 87 - 229 1.3e-09 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 91 - 249 3.2e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 93 - 263 5.7e-14 WDR5 beta-propeller domain
WD40_Prp19 PF24814 111 - 252 4e-06 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 121 - 251 3.2e-12 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 134 - 303 7.5e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 171 - 307 2.8e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 195 - 336 1.6e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 217 - 334 1.8e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 242 - 337 7.6e-11 WDR5 beta-propeller domain
TPR_GEMI5 PF23774 539 - 690 5.4e-08 GEMI5 TPR domain
TPR_Sec16 PF12931 558 - 754 4.8e-08 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63460 AT3G63460 AT3G63460
fragaria_vesca FvH4_7g01850 FvH4_7g01850 FvH4_7g01850
malus_domestica MD02G1300000.v1.1 MD07G1024900.v1.1
prunus_persica Prupe.2G024600_v2.0.a1
pyrus_communis pycom02g25200 pycom07g01920
rosa_chinensis RchiOBHm_Chr1g0318481
rosa_laevigata RLG00000030534
rosa_multiflora Rmu_ssc0000021.1_g000001
rosa_roxburghii Rroxscaffold_4G00329170
rosa_rugosa Rorug01G0021600.1
rosa_samantha Rh1AG034000 Rh1BG030000 Rh1CG033500 Rh1DG037100
rosa_wichuraiana Rw1G002700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2059
Acc36I ACCTGC 1 cut(s) 916
Acc65I GGTACC 1 cut(s) 942
AccB1I GGYRCC 2 cut(s) 813, 942
AccB7I CCANNNNNTGG 3 cut(s) 2825, 3184, 3243
AccBSI CCGCTC 2 cut(s) 196, 2113
AccI GTMKAC 1 cut(s) 276
AccII CGCG 2 cut(s) 159, 771
AclI AACGTT 4 cut(s) 681, 2442, 2976, 3008
AclWI GGATC 7 cut(s) 21, 228, 235, 241, 1428, 1500, 1752
AcoI YGGCCR 5 cut(s) 66, 81, 346, 1770, 2244
AcsI RAATTY 5 cut(s) 242, 438, 925, 1229, 2085
AcuI CTGAAG 5 cut(s) 450, 1314, 2361, 2675, 3184
AcvI CACGTG 1 cut(s) 3056
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 9 cut(s) 944, 1051, 1156, 1804, 1822, 1957, 2279, 2483, 3225
AhdI GACNNNNNGTC 1 cut(s) 145
AjnI CCWGG 7 cut(s) 42, 212, 955, 1191, 2743, 2857, 3296
AjuI GAANNNNNNNTTGG 4 cut(s) 234, 266, 2674, 2706
Alw21I GWGCWC 2 cut(s) 659, 3112
Alw26I GTCTC 6 cut(s) 1292, 1316, 1931, 2354, 3023, 3129
AlwI GGATC 7 cut(s) 21, 228, 235, 241, 1428, 1500, 1752
AlwNI CAGNNNCTG 3 cut(s) 2357, 2825, 2927
Ama87I CYCGRG 3 cut(s) 122, 2737, 3094
ApaI GGGCCC 1 cut(s) 1144
ApoI RAATTY 5 cut(s) 242, 438, 925, 1229, 2085
ArsI GACNNNNNNTTYG 2 cut(s) 1673, 1705
Asp718I GGTACC 1 cut(s) 942
AspLEI GCGC 8 cut(s) 771, 1086, 1855, 2060, 2204, 2434, 2932, 3053
AspS9I GGNCC 8 cut(s) 22, 368, 1140, 1141, 2708, 2741, 2765, 2828
AsuC2I CCSGG 3 cut(s) 1088, 2738, 2739
AsuHPI GGTGA 8 cut(s) 446, 530, 714, 753, 832, 1225, 1894, 2301
AsuII TTCGAA 1 cut(s) 1350
AvaI CYCGRG 3 cut(s) 122, 2737, 3094
AvaII GGWCC 3 cut(s) 2708, 2741, 2828
BaeGI GKGCMC 2 cut(s) 818, 1144
BaeI ACNNNNGTAYC 4 cut(s) 1566, 1566, 1599, 1599
BalI TGGCCA 2 cut(s) 348, 2246
BamHI GGATCC 1 cut(s) 233
BanI GGYRCC 2 cut(s) 813, 942
BanII GRGCYC 2 cut(s) 1144, 1440
BauI CACGAG 1 cut(s) 1824
BbrPI CACGTG 1 cut(s) 3056
BbsI GAAGAC 4 cut(s) 1548, 1847, 2165, 3093
Bbv12I GWGCWC 2 cut(s) 659, 3112
BceAI ACGGC 2 cut(s) 74, 2283
BcgI CGANNNNNNTGC 6 cut(s) 636, 670, 849, 883, 1499, 1533
BciT130I CCWGG 7 cut(s) 44, 214, 957, 1193, 2745, 2859, 3298
BciVI GTATCC 3 cut(s) 868, 1465, 1567
BclI TGATCA 1 cut(s) 1380
BcnI CCSGG 3 cut(s) 1088, 2738, 2739
BcoDI GTCTC 6 cut(s) 1292, 1316, 1931, 2354, 3023, 3129
BfmI CTRYAG 4 cut(s) 2037, 2349, 2370, 2961
BfoI RGCGCY 1 cut(s) 1087
BfuAI ACCTGC 1 cut(s) 916
BfuI GTATCC 3 cut(s) 868, 1465, 1567
BglI GCCNNNNNGGC 1 cut(s) 1392
BglII AGATCT 6 cut(s) 126, 289, 1447, 2123, 2146, 2873
BlpI GCTNAGC 3 cut(s) 2133, 2559, 2607
BmcAI AGTACT 2 cut(s) 1822, 2279
Bme18I GGWCC 3 cut(s) 2708, 2741, 2828
BmeRI GACNNNNNGTC 1 cut(s) 145
BmeT110I CYCGRG 3 cut(s) 122, 2737, 3094
BmgT120I GGNCC 8 cut(s) 22, 368, 1140, 1141, 2708, 2741, 2765, 2828
BmrI ACTGGG 2 cut(s) 1679, 3053
BmuI ACTGGG 2 cut(s) 1679, 3053
BoxI GACNNNNGTC 1 cut(s) 2165
BpiI GAAGAC 4 cut(s) 1548, 1847, 2165, 3093
BpmI CTGGAG 4 cut(s) 978, 2571, 2802, 2841
Bpu10I CCTNAGC 1 cut(s) 849
Bpu1102I GCTNAGC 3 cut(s) 2133, 2559, 2607
Bpu14I TTCGAA 1 cut(s) 1350
BpuEI CTTGAG 4 cut(s) 404, 476, 625, 2490
BpuMI CCSGG 3 cut(s) 1088, 2738, 2739
BsaAI YACGTR 2 cut(s) 2330, 3056
BsaBI GATNNNNATC 2 cut(s) 232, 2670
BsaHI GRCGYC 1 cut(s) 649
BsaWI WCCGGW 1 cut(s) 1570
BsaXI ACNNNNNCTCC 2 cut(s) 2575, 2605
Bse118I RCCGGY 2 cut(s) 263, 651
Bse1I ACTGG 6 cut(s) 784, 1494, 1685, 2188, 2247, 3048
Bse3DI GCAATG 4 cut(s) 810, 2071, 2277, 2608
Bse8I GATNNNNATC 2 cut(s) 232, 2670
BseBI CCWGG 7 cut(s) 44, 214, 957, 1193, 2745, 2859, 3298
BseGI GGATG 5 cut(s) 1701, 2122, 2515, 2612, 3288
BseJI GATNNNNATC 2 cut(s) 232, 2670
BseMI GCAATG 4 cut(s) 810, 2071, 2277, 2608
BseNI ACTGG 6 cut(s) 784, 1494, 1685, 2188, 2247, 3048
BseRI GAGGAG 3 cut(s) 1328, 1940, 2110
BseSI GKGCMC 2 cut(s) 818, 1144
BseYI CCCAGC 4 cut(s) 185, 321, 326, 870
BsgI GTGCAG 2 cut(s) 2634, 2898
Bsh1236I CGCG 2 cut(s) 159, 771
Bsh1285I CGRYCG 1 cut(s) 157
BshNI GGYRCC 2 cut(s) 813, 942
BsiEI CGRYCG 1 cut(s) 157
BsiHKAI GWGCWC 2 cut(s) 659, 3112
BsiHKCI CYCGRG 3 cut(s) 122, 2737, 3094
BsiSI CCGG 7 cut(s) 72, 84, 264, 652, 1088, 1571, 2738
BslFI GGGAC 7 cut(s) 464, 598, 614, 755, 2486, 2721, 3260
BsmAI GTCTC 6 cut(s) 1292, 1316, 1931, 2354, 3023, 3129
BsmFI GGGAC 7 cut(s) 464, 598, 614, 755, 2486, 2721, 3260
BsmI GAATGC 4 cut(s) 1164, 2665, 3154, 3257
BsoBI CYCGRG 3 cut(s) 122, 2737, 3094
Bsp119I TTCGAA 1 cut(s) 1350
Bsp120I GGGCCC 1 cut(s) 1140
Bsp1286I GDGCHC 5 cut(s) 659, 818, 1144, 1440, 3112
Bsp1720I GCTNAGC 3 cut(s) 2133, 2559, 2607
Bsp19I CCATGG 2 cut(s) 78, 2847
Bsp68I TCGCGA 1 cut(s) 159
BspFNI CGCG 2 cut(s) 159, 771
BspMAI CTGCAG 3 cut(s) 2041, 2353, 2965
BspMI ACCTGC 1 cut(s) 916
BspPI GGATC 7 cut(s) 21, 228, 235, 241, 1428, 1500, 1752
BspQI GCTCTTC 1 cut(s) 115
BspT104I TTCGAA 1 cut(s) 1350
BspT107I GGYRCC 2 cut(s) 813, 942
BsrBI CCGCTC 2 cut(s) 196, 2113
BsrDI GCAATG 4 cut(s) 810, 2071, 2277, 2608
BsrFI RCCGGY 2 cut(s) 263, 651
BsrI ACTGG 6 cut(s) 784, 1494, 1685, 2188, 2247, 3048
BssAI RCCGGY 2 cut(s) 263, 651
BssNI GRCGYC 1 cut(s) 649
BssSI CACGAG 1 cut(s) 1824
BssT1I CCWWGG 4 cut(s) 78, 250, 2251, 2847
Bst2BI CACGAG 1 cut(s) 1824
Bst2UI CCWGG 7 cut(s) 44, 214, 957, 1193, 2745, 2859, 3298
Bst4CI ACNGT 7 cut(s) 592, 669, 719, 1505, 2080, 2481, 2953
Bst6I CTCTTC 6 cut(s) 115, 1029, 1290, 1305, 1347, 1659
BstACI GRCGYC 1 cut(s) 649
BstAPI GCANNNNNTGC 3 cut(s) 1654, 2927, 3307
BstBAI YACGTR 2 cut(s) 2330, 3056
BstBI TTCGAA 1 cut(s) 1350
BstDSI CCRYGG 2 cut(s) 78, 2847
BstF5I GGATG 5 cut(s) 1701, 2122, 2515, 2612, 3288
BstFNI CGCG 2 cut(s) 159, 771
BstH2I RGCGCY 1 cut(s) 1087
BstHHI GCGC 8 cut(s) 771, 1086, 1855, 2060, 2204, 2434, 2932, 3053
BstMAI GTCTC 6 cut(s) 1292, 1316, 1931, 2354, 3023, 3129
BstMCI CGRYCG 1 cut(s) 157
BstNI CCWGG 7 cut(s) 44, 214, 957, 1193, 2745, 2859, 3298
BstPAI GACNNNNGTC 1 cut(s) 2165
BstSFI CTRYAG 4 cut(s) 2037, 2349, 2370, 2961
BstSLI GKGCMC 2 cut(s) 818, 1144
BstUI CGCG 2 cut(s) 159, 771
BstV2I GAAGAC 4 cut(s) 1548, 1847, 2165, 3093
BstX2I RGATCY 9 cut(s) 126, 227, 233, 289, 1447, 1492, 2123, 2146, 2873
BstYI RGATCY 9 cut(s) 126, 227, 233, 289, 1447, 1492, 2123, 2146, 2873
BsuI GTATCC 3 cut(s) 868, 1465, 1567
BtgI CCRYGG 2 cut(s) 78, 2847
BtgZI GCGATG 2 cut(s) 1541, 1892
BtsCI GGATG 5 cut(s) 1701, 2122, 2515, 2612, 3288
BtsI GCAGTG 2 cut(s) 485, 1855
BtsIMutI CAGTG 8 cut(s) 485, 674, 1487, 1754, 1855, 2484, 2651, 3041
BtuMI TCGCGA 1 cut(s) 159
BveI ACCTGC 1 cut(s) 916
CaiI CAGNNNCTG 3 cut(s) 2357, 2825, 2927
CfoI GCGC 8 cut(s) 771, 1086, 1855, 2060, 2204, 2434, 2932, 3053
Cfr10I RCCGGY 2 cut(s) 263, 651
Cfr13I GGNCC 8 cut(s) 22, 368, 1140, 1141, 2708, 2741, 2765, 2828
Cfr9I CCCGGG 1 cut(s) 2737
CseI GACGC 1 cut(s) 657
Csp6I GTAC 9 cut(s) 943, 1050, 1155, 1803, 1821, 1956, 2278, 2482, 3224
CspCI CAANNNNNGTGG 2 cut(s) 481, 516
CviQI GTAC 9 cut(s) 943, 1050, 1155, 1803, 1821, 1956, 2278, 2482, 3224
DraI TTTAAA 1 cut(s) 1062
DriI GACNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 5 cut(s) 66, 81, 346, 1770, 2244
Eam1104I CTCTTC 6 cut(s) 115, 1029, 1290, 1305, 1347, 1659
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 6 cut(s) 115, 1029, 1290, 1305, 1347, 1659
EciI GGCGGA 3 cut(s) 19, 103, 2931
Eco130I CCWWGG 4 cut(s) 78, 250, 2251, 2847
Eco24I GRGCYC 2 cut(s) 1144, 1440
Eco47I GGWCC 3 cut(s) 2708, 2741, 2828
Eco57I CTGAAG 5 cut(s) 450, 1314, 2361, 2675, 3184
Eco72I CACGTG 1 cut(s) 3056
Eco88I CYCGRG 3 cut(s) 122, 2737, 3094
EcoO109I RGGNCCY 4 cut(s) 368, 1140, 1141, 2741
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 7 cut(s) 42, 212, 955, 1191, 2743, 2857, 3296
EcoT14I CCWWGG 4 cut(s) 78, 250, 2251, 2847
EcoT22I ATGCAT 1 cut(s) 2725
EcoT38I GRGCYC 2 cut(s) 1144, 1440
ErhI CCWWGG 4 cut(s) 78, 250, 2251, 2847
FalI AAGNNNNNCTT 6 cut(s) 125, 157, 1926, 1958, 2244, 2276
FaqI GGGAC 7 cut(s) 464, 598, 614, 755, 2486, 2721, 3260
FauI CCCGC 1 cut(s) 2106
FauNDI CATATG 1 cut(s) 2458
FbaI TGATCA 1 cut(s) 1380
FblI GTMKAC 1 cut(s) 276
FokI GGATG 5 cut(s) 1688, 2129, 2502, 2599, 3295
FriOI GRGCYC 2 cut(s) 1144, 1440
FspI TGCGCA 1 cut(s) 2059
GlaI GCGC 8 cut(s) 770, 1085, 1854, 2059, 2203, 2433, 2931, 3052
GsaI CCCAGC 4 cut(s) 189, 325, 330, 874
GsuI CTGGAG 4 cut(s) 978, 2571, 2802, 2841
HaeII RGCGCY 1 cut(s) 1087
HapII CCGG 7 cut(s) 72, 84, 264, 652, 1088, 1571, 2738
HgaI GACGC 1 cut(s) 657
HhaI GCGC 8 cut(s) 771, 1086, 1855, 2060, 2204, 2434, 2932, 3053
Hin1I GRCGYC 1 cut(s) 649
Hin6I GCGC 8 cut(s) 769, 1084, 1853, 2058, 2202, 2432, 2930, 3051
HinP1I GCGC 8 cut(s) 769, 1084, 1853, 2058, 2202, 2432, 2930, 3051
HincII GTYRAC 3 cut(s) 277, 2071, 3012
HindII GTYRAC 3 cut(s) 277, 2071, 3012
HindIII AAGCTT 1 cut(s) 1118
HinfI GANTC 9 cut(s) 207, 298, 638, 1216, 1304, 1939, 2161, 2294, 2489
HpaII CCGG 7 cut(s) 72, 84, 264, 652, 1088, 1571, 2738
HphI GGTGA 8 cut(s) 446, 530, 714, 753, 832, 1225, 1894, 2301
Hpy166II GTNNAC 9 cut(s) 277, 361, 722, 777, 1885, 2071, 2216, 2484, 3012
Hpy8I GTNNAC 9 cut(s) 277, 361, 722, 777, 1885, 2071, 2216, 2484, 3012
Hpy99I CGWCG 4 cut(s) 95, 176, 281, 651
HpyAV CCTTC 9 cut(s) 474, 483, 1352, 1620, 1858, 2646, 2769, 2778, 3221
HpyCH4III ACNGT 7 cut(s) 592, 669, 719, 1505, 2080, 2481, 2953
HpyCH4IV ACGT 6 cut(s) 681, 2329, 2442, 2976, 3008, 3055
HpySE526I ACGT 6 cut(s) 681, 2329, 2442, 2976, 3008, 3055
Hsp92I GRCGYC 1 cut(s) 649
HspAI GCGC 8 cut(s) 769, 1084, 1853, 2058, 2202, 2432, 2930, 3051
KpnI GGTACC 1 cut(s) 946
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 1380
LguI GCTCTTC 1 cut(s) 115
MaeII ACGT 6 cut(s) 681, 2329, 2442, 2976, 3008, 3055
MbiI CCGCTC 2 cut(s) 196, 2113
MfeI CAATTG 1 cut(s) 919
MflI RGATCY 9 cut(s) 126, 227, 233, 289, 1447, 1492, 2123, 2146, 2873
MhlI GDGCHC 5 cut(s) 659, 818, 1144, 1440, 3112
MlsI TGGCCA 2 cut(s) 348, 2246
MluNI TGGCCA 2 cut(s) 348, 2246
MlyI GAGTC 4 cut(s) 201, 1225, 1933, 2155
MmeI TCCRAC 3 cut(s) 1702, 2921, 2997
Mox20I TGGCCA 2 cut(s) 348, 2246
Mph1103I ATGCAT 1 cut(s) 2725
MroNI GCCGGC 1 cut(s) 263
MscI TGGCCA 2 cut(s) 348, 2246
MseI TTAA 6 cut(s) 132, 390, 458, 627, 1061, 1551
MslI CAYNNNNRTG 5 cut(s) 1072, 1797, 2209, 2543, 3172
Msp20I TGGCCA 2 cut(s) 348, 2246
MspA1I CMGCKG 4 cut(s) 1517, 2825, 2912, 3132
MspI CCGG 7 cut(s) 72, 84, 264, 652, 1088, 1571, 2738
MunI CAATTG 1 cut(s) 919
Mva1269I GAATGC 4 cut(s) 1164, 2665, 3154, 3257
MvaI CCWGG 7 cut(s) 44, 214, 957, 1193, 2745, 2859, 3298
MvnI CGCG 2 cut(s) 159, 771
NaeI GCCGGC 1 cut(s) 265
NciI CCSGG 3 cut(s) 1088, 2738, 2739
NcoI CCATGG 2 cut(s) 78, 2847
NdeI CATATG 1 cut(s) 2458
NgoMIV GCCGGC 1 cut(s) 263
NmuCI GTSAC 5 cut(s) 759, 821, 1130, 1917, 2307
NruI TCGCGA 1 cut(s) 159
NsbI TGCGCA 1 cut(s) 2059
NsiI ATGCAT 1 cut(s) 2725
NspV TTCGAA 1 cut(s) 1350
PaeR7I CTCGAG 2 cut(s) 122, 3094
PciSI GCTCTTC 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 1506
PctI GAATGC 4 cut(s) 1164, 2665, 3154, 3257
PdiI GCCGGC 1 cut(s) 265
PfeI GAWTC 5 cut(s) 298, 638, 1304, 2294, 2489
PflMI CCANNNNNTGG 3 cut(s) 2825, 3184, 3243
PfoI TCCNGGA 1 cut(s) 2857
Ple19I CGATCG 1 cut(s) 157
PleI GAGTC 4 cut(s) 201, 1224, 1933, 2155
PmaCI CACGTG 1 cut(s) 3056
PmlI CACGTG 1 cut(s) 3056
PpsI GAGTC 4 cut(s) 201, 1224, 1933, 2155
Ppu21I YACGTR 2 cut(s) 2330, 3056
PpuMI RGGWCCY 1 cut(s) 2741
PshAI GACNNNNGTC 1 cut(s) 2165
Psp1406I AACGTT 4 cut(s) 681, 2442, 2976, 3008
Psp5II RGGWCCY 1 cut(s) 2741
Psp6I CCWGG 7 cut(s) 42, 212, 955, 1191, 2743, 2857, 3296
PspCI CACGTG 1 cut(s) 3056
PspFI CCCAGC 4 cut(s) 185, 321, 326, 870
PspGI CCWGG 7 cut(s) 42, 212, 955, 1191, 2743, 2857, 3296
PspOMI GGGCCC 1 cut(s) 1140
PspPI GGNCC 8 cut(s) 22, 368, 1140, 1141, 2708, 2741, 2765, 2828
PspPPI RGGWCCY 1 cut(s) 2741
PstI CTGCAG 3 cut(s) 2041, 2353, 2965
PstNI CAGNNNCTG 3 cut(s) 2357, 2825, 2927
PsuI RGATCY 9 cut(s) 126, 227, 233, 289, 1447, 1492, 2123, 2146, 2873
PvuI CGATCG 1 cut(s) 157
PvuII CAGCTG 3 cut(s) 1517, 2825, 2912
RruI TCGCGA 1 cut(s) 159
RsaI GTAC 9 cut(s) 944, 1051, 1156, 1804, 1822, 1957, 2279, 2483, 3225
RsaNI GTAC 9 cut(s) 943, 1050, 1155, 1803, 1821, 1956, 2278, 2482, 3224
RseI CAYNNNNRTG 5 cut(s) 1072, 1797, 2209, 2543, 3172
SalI GTCGAC 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 6 cut(s) 132, 390, 458, 627, 1061, 1551
Sau96I GGNCC 8 cut(s) 22, 368, 1140, 1141, 2708, 2741, 2765, 2828
ScaI AGTACT 2 cut(s) 1822, 2279
SchI GAGTC 4 cut(s) 201, 1225, 1933, 2155
SduI GDGCHC 5 cut(s) 659, 818, 1144, 1440, 3112
SfcI CTRYAG 4 cut(s) 2037, 2349, 2370, 2961
Sfr274I CTCGAG 2 cut(s) 122, 3094
SfuI TTCGAA 1 cut(s) 1350
SgrAI CRCCGGYG 1 cut(s) 651
SinI GGWCC 3 cut(s) 2708, 2741, 2828
SlaI CTCGAG 2 cut(s) 122, 3094
SmaI CCCGGG 1 cut(s) 2739
SmiMI CAYNNNNRTG 5 cut(s) 1072, 1797, 2209, 2543, 3172
SmlI CTYRAG 6 cut(s) 122, 383, 491, 604, 2505, 3094
SmoI CTYRAG 6 cut(s) 122, 383, 491, 604, 2505, 3094
SspI AATATT 2 cut(s) 1835, 2325
StyI CCWWGG 4 cut(s) 78, 250, 2251, 2847
TaaI ACNGT 7 cut(s) 592, 669, 719, 1505, 2080, 2481, 2953
TaiI ACGT 6 cut(s) 684, 2332, 2445, 2979, 3011, 3058
TatI WGTACW 3 cut(s) 1820, 1955, 2277
TauI GCSGC 3 cut(s) 71, 270, 1481
TfiI GAWTC 5 cut(s) 298, 638, 1304, 2294, 2489
Tru1I TTAA 6 cut(s) 132, 390, 458, 627, 1061, 1551
Tru9I TTAA 6 cut(s) 132, 390, 458, 627, 1061, 1551
TscAI CASTG 8 cut(s) 492, 674, 1494, 1754, 1855, 2491, 2658, 3048
TseFI GTSAC 5 cut(s) 759, 821, 1130, 1917, 2307
Tsp45I GTSAC 5 cut(s) 759, 821, 1130, 1917, 2307
TspDTI ATGAA 5 cut(s) 926, 966, 1877, 2288, 2688
TspGWI ACGGA 3 cut(s) 25, 240, 294
TspMI CCCGGG 1 cut(s) 2737
TspRI CASTG 8 cut(s) 492, 674, 1494, 1754, 1855, 2491, 2658, 3048
Van91I CCANNNNNTGG 3 cut(s) 2825, 3184, 3243
VpaK11BI GGWCC 3 cut(s) 2708, 2741, 2828
XapI RAATTY 5 cut(s) 242, 438, 925, 1229, 2085
XhoI CTCGAG 2 cut(s) 122, 3094
XmaI CCCGGG 1 cut(s) 2737
XmiI GTMKAC 1 cut(s) 276
ZrmI AGTACT 2 cut(s) 1822, 2279
Zsp2I ATGCAT 1 cut(s) 2725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.