MD07G1024900.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010028
Physical Location & Seq
Forward (+)
2077503 .. 2093906
16404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1024900.v1.1.491

Sequence Viewer

Length: 3336 bp
ATGGCGTGCGTAAAGGGGGTGAATCGATCGGCCTGCGTGGCGTTTGCTCCGGACGCGCCGTACATGGCGGCAGGGACGATGGCCGGAGCAGTGGATCTGTCCTTCAGCTCCTCCGCCAACATTGAGATCTTCAAGCTTGATTTACAGTCAGAAGATCGTGATCTCCCGGTAGTGGGCGAGTCGACGAGCTCTGAGAGATTCAACAGGTTGTCGTGGGCCAAACCCACTGGATCGGGGTCTCAAGAATTCGGGCTTGGGCTCATTGCTGGTGGACTCGTTGATGGCACTATTGATATCTGGAACCCACAGACTCTGATCCGGGAAATGGGGTTAAAGTTAGCTGATATTGCGGTTTATTGTTGTAGTGTTCGTGGTCTTGAATTTAATGCAATTGCACCCAATTTGCTTGCATCTGGGGCTGATGATGGTGAAATTTGCATATGGGACATGACCAATCCTGCAGAACCATCTCACTTTCCACCTCTCAAGGGTAGTGGTTCTGCCGCCCAGGGTGAGGTTTCATTTTTATCTTGGAATAGCAAGGTCCAACATATATTAGCATCCACTTCATTAAATGGGACAACTGTGATATGGGACCTGAAAAAGCAAAAGCCAGTGATAAGTTTTACAGATTCAGTTAGAAGACGGTGCTCCGTTTTGCAGTGGAATCCTGATATTGCCACTCAGCTAGTTGTTGCTTCAGATGAAGATGGTTCACCGTCTCTGAGGCTGTGGGATATGCGTAATATAATGTCACCAGTTAAAGAGTTTGTCGGGCACACAAAAGGTGTAATTGCAATGTCTTGGTGTCCGAATGACAGCTCTTATCTGCTTACATGTGCCAAGGATAATAGAACTATTTGCTGGGACACAGTTTCTGCAGAGATTGTATGTGAATTGCCGGCAGGCACCAATTGGAATTTTGATGTGCATTGGTATCCAAAAGTACCTGGAGTGATTGCGGCATCTTCATTTGATGGAAAAATTGGAATTTATAATATTGAGGGTTGCAGCAGATATGGAGTTGGGGAAAGTGCTTTTGGTGCAGGACCTCTGAGAGCTCCAAAATGGTATAAGCGCCCAGTTGGAGCATCTTTTGGCTTTGGAGGCAAGATTGTGTCCTTTCAGCATGGTTCTGCTGGTTCGGAGGTTTATATACACAGCTTGGTGACTGAACACAGTTTGGTGAATCGCTCATCCGAATTTGAAGCTGCTATACAAAATGGGGAAAGGCCTTTACTTCGAGCCTTATGTGAGAAGAAATCACAGGAGGCTGAATCTGAGGATGACCAGGAAACATGGGGCTTATTAAGGGTTATGTTTGAAGATGATGGGACTGCAAGGACGAAGCTGATCACTCATCTTGGTTTCAGTATGCCCGAGGAAGCAAAAGAAGATGTTCCAGAAGATCTATCACAGGAAGTCAATGCACTAGGGCTTGAGGATACAATAACTGATAAAGTGGGTCGTGGGAATGATAAGGAAGCCACCATCTTTCCATCTGATAATGGAGAAGATTTCTTTAATAATCTTCCTAGTCCTAAAGCTGATACTCCTGTGTCAACCTCTGGTGGAAAATTTTCTGAGGGGGATGCAGTTCCTGTTATGGAACAAATGGAACAAGAGCCTGATGGACAGGAGGAGAGTGCTGATCCATCGTTTGATGAATCTGTTCAACATGCTTTAGTTGTAGGAGATTATAAGGGTGCTGTTGCAAAATGCATTTCAGTAAATAAAATGGCTGATGCTTTAGTTATTGCTCATGCTGGTGGTGCTTCGTTGTGGGAGAGCACGCGGGATCAGTATCTTAAGATGAGCCATTCGCCTTACCTTAAGATTGTTTCTGCAATGGTGAGCAACGATCTCTTGAGTCTTGTCAACACCAGGCCCCTAAAATTCTGGAAAGAAACACTTGCTCTTCTGTGTAGTTTTGCGTCCAGAGATGAGTGGACCATGCTTTGTGATACACTTGCTTCCAAACTTGTAGCTGCTGGTAATACTCTGGCGGCAACTATTTGTTATATATGTGCGGGAAATATTGATAAAACTGTAGAGATTTGGTCAAGAAGTCTGACAACTGAGCATGAAGGGAGATCCTATGTTGACCTTCTCCAGGAATTAATGGAAAAGACAATTGTCCTTGCCTTGGCATCTGGGCAGAAGCGGTTTAGTGCATCCTTGTGCAAACTTGTTGAGAAATACGCTGAAATTTTAGCTAGTCAAGGCCTTCTAACAACAGCATTGGAGTACCTAAAACTATTAGGTTCTGATGAGTTGTCACCTGAGCTTGTTATCTTAAGAGATCGTATTTCTCTTTCAACCGAACCTGAGAAAGTTACCAAGACTGAAACATTTGGAAACCAACCTGCAACAAGTGGGCCTGAATACACGGCTGACCAATCTAGTTTTGTCGGGTCCTCTCCTCCATATTATCAGGAAACAGTGCCATCACATTTGCAATCAGGTGTTCCTGTAAGTCCTTATGGTGAGAGCTACCAAGAACCTGTGAATCCTTCATATGGAAGAGGAGGATACGGCCCTCCTGCCTCGTATCAACCTGCATCCCAGCCCCATATGTTTCTTCCTAACCAACCACCGCAGGTTCCACAGGAGAAATTTTCTGCACCTCCTGTTTCCTCTCAGCCTGCAGTAAGGTCTTTCATTCCTTCAACTCCTCCTGTGCTGAAAAATGTGGATCAATATCAGCAGCCAACATTGGGTTCTCAGTTGTATCCTACTTTCCAACCTATGCAACCAGGGCCTGGTTCAACTGCTCCTCTTCAATCACATGTCCCTCCTGTTCCTGTAAATCAACCACATGTTGTAGCTGCTTCTGTTCCTCCAAGAGGATTTATGCCCGTTACTAATCCAGGAGTTGTTCAGGGGCCTCATGTGGGTTCACTGCAGCCACCTAGTCCTACTCACCAAGCTCCAGCTCGAACCCCCGTGGCAGCGGCAGCTCCACCGCCCACCATACAGACAGTTGATACAGCCAATGTTCCTGCCCATCAAAAATCGGTAATCGCAACATTAACAAGACTTTTCAATGAGACATCGGAGGCGTTGGGAGGTGCACGTGCAAATCCCGGTAAGAAGCGTGAAATAGAAGACAATTCAAGGAAGATAGGTGCATTGTTTGCCAAACTCAACAGTGGGGACATATCTAGAAATGCTGCTGATAAGCTTGTTCAGCTTTGCCAGGCATTGGATAATGGTGATTTCGGTACTGCCCTGCAAATCCAGGTGCTTCTTACCACTAGTGAATGGGATGAGTGCAACTTTTGGCTCGCAACTCTAAAGAGGATGATCAAGACAAGGCAAAATGTGAGACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SRA1 Sec16_C WD40

Protein Analysis

1112

Amino Acids

120.09

Weight (kDa)

5.1

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 88 - 248 9.8e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 88 - 203 1.7e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 89 - 292 7.4e-10 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 100 - 248 3e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 123 - 249 1.6e-10 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 134 - 297 6.8e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 169 - 303 2e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 194 - 335 2.4e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 216 - 334 5e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 242 - 335 9.6e-10 WDR5 beta-propeller domain
TPR_GEMI5 PF23774 535 - 691 7e-07 GEMI5 TPR domain
TPR_Sec16 PF12931 558 - 753 1.1e-08 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63460 AT3G63460 AT3G63460
fragaria_vesca FvH4_7g01850 FvH4_7g01850 FvH4_7g01850
malus_domestica MD02G1300000.v1.1 MD07G1024900.v1.1
prunus_persica Prupe.2G024600_v2.0.a1
pyrus_communis pycom02g25200 pycom07g01920
rosa_chinensis RchiOBHm_Chr1g0318481
rosa_laevigata RLG00000030534
rosa_multiflora Rmu_ssc0000021.1_g000001
rosa_roxburghii Rroxscaffold_4G00329170
rosa_rugosa Rorug01G0021600.1
rosa_samantha Rh1AG034000 Rh1BG030000 Rh1CG033500 Rh1DG037100
rosa_wichuraiana Rw1G002700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 996, 1701
Acc36I ACCTGC 3 cut(s) 2374, 2566, 2590
AccB1I GGYRCC 1 cut(s) 908
AccB7I CCANNNNNTGG 2 cut(s) 2762, 3205
AccI GTMKAC 1 cut(s) 182
AccII CGCG 2 cut(s) 56, 1795
AccIII TCCGGA 1 cut(s) 49
AclWI GGATC 7 cut(s) 102, 238, 310, 1646, 1806, 2088, 2703
AcoI YGGCCR 1 cut(s) 81
AcuI CTGAAG 2 cut(s) 88, 684
AcvI CACGTG 1 cut(s) 3077
AfaI GTAC 4 cut(s) 62, 948, 2249, 3226
AflII CTTAAG 3 cut(s) 1808, 1832, 2296
AflIII ACRYGT 3 cut(s) 836, 2788, 2818
AhlI ACTAGT 1 cut(s) 3257
AjuI GAANNNNNNNTTGG 6 cut(s) 237, 269, 1023, 1055, 2704, 2736
Alw21I GWGCWC 5 cut(s) 191, 653, 1063, 1793, 3076
Alw26I GTCTC 4 cut(s) 243, 726, 3044, 3322
Alw44I GTGCAC 1 cut(s) 3072
AlwI GGATC 7 cut(s) 102, 238, 310, 1646, 1806, 2088, 2703
AlwNI CAGNNNCTG 4 cut(s) 313, 878, 1601, 2762
Ama87I CYCGRG 1 cut(s) 1379
Aor13HI TCCGGA 1 cut(s) 49
ApaLI GTGCAC 1 cut(s) 3072
ApeKI GCWGC 9 cut(s) 1011, 1211, 1988, 2707, 2828, 2905, 2951, 2957, 3173
AseI ATTAAT 1 cut(s) 2120
Asp700I GAANNNNTTC 3 cut(s) 1398, 1579, 1671
AspLEI GCGC 2 cut(s) 58, 1080
AsuC2I CCSGG 3 cut(s) 167, 320, 3087
AvaI CYCGRG 1 cut(s) 1379
AvaII GGWCC 5 cut(s) 544, 595, 1049, 1950, 2415
BaeGI GKGCMC 2 cut(s) 780, 3076
BaeI ACNNNNGTAYC 2 cut(s) 1542, 1575
BanI GGYRCC 1 cut(s) 908
BanII GRGCYC 3 cut(s) 191, 261, 1063
BarI GAAGNNNNNNTAC 2 cut(s) 1200, 1232
BbrPI CACGTG 1 cut(s) 3077
BbsI GAAGAC 2 cut(s) 649, 3114
Bbv12I GWGCWC 5 cut(s) 191, 653, 1063, 1793, 3076
BbvI GCAGC 9 cut(s) 1023, 1198, 1975, 2719, 2815, 2917, 2963, 2969, 3160
BceAI ACGGC 3 cut(s) 43, 2406, 2551
BcgI CGANNNNNNTGC 2 cut(s) 15, 49
BciVI GTATCC 4 cut(s) 948, 1438, 2525, 2742
BclI TGATCA 2 cut(s) 1353, 3306
BcnI CCSGG 3 cut(s) 167, 320, 3087
BcoDI GTCTC 4 cut(s) 243, 726, 3044, 3322
BcuI ACTAGT 1 cut(s) 3257
BfaI CTAG 8 cut(s) 689, 1433, 1536, 2217, 2403, 2913, 3165, 3258
BfmI CTRYAG 5 cut(s) 459, 879, 2049, 2646, 2903
BfoI RGCGCY 1 cut(s) 1081
BfrI CTTAAG 3 cut(s) 1808, 1832, 2296
BfuAI ACCTGC 3 cut(s) 2374, 2566, 2590
BfuI GTATCC 4 cut(s) 948, 1438, 2525, 2742
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 2 cut(s) 126, 1408
Bme18I GGWCC 5 cut(s) 544, 595, 1049, 1950, 2415
BmeT110I CYCGRG 1 cut(s) 1379
BmiI GGNNCC 7 cut(s) 302, 596, 910, 1889, 2416, 2604, 2886
BmrI ACTGGG 1 cut(s) 1076
BmsI GCATC 9 cut(s) 419, 569, 974, 1100, 1582, 1735, 2159, 2183, 2570
BmuI ACTGGG 1 cut(s) 1076
BoxI GACNNNNGTC 1 cut(s) 2135
BpiI GAAGAC 2 cut(s) 649, 3114
BpmI CTGGAG 3 cut(s) 972, 2096, 2916
Bpu10I CCTNAGC 1 cut(s) 2283
BpuEI CTTGAG 4 cut(s) 225, 470, 1460, 1888
BpuMI CCSGG 3 cut(s) 167, 320, 3087
Bsa29I ATCGAT 1 cut(s) 25
BsaAI YACGTR 1 cut(s) 3077
BsaBI GATNNNNATC 3 cut(s) 159, 1803, 2700
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 7 cut(s) 507, 508, 843, 1380, 2145, 2756, 2946
BsaWI WCCGGW 1 cut(s) 49
Bse118I RCCGGY 1 cut(s) 901
Bse1I ACTGG 4 cut(s) 232, 614, 758, 1082
Bse3DI GCAATG 3 cut(s) 261, 804, 1854
Bse8I GATNNNNATC 3 cut(s) 159, 1803, 2700
BseAI TCCGGA 1 cut(s) 49
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 7 cut(s) 507, 508, 843, 1380, 2145, 2756, 2946
BseGI GGATG 8 cut(s) 560, 1196, 1291, 1597, 2174, 2561, 3274, 3309
BseJI GATNNNNATC 3 cut(s) 159, 1803, 2700
BseMI GCAATG 3 cut(s) 261, 804, 1854
BseNI ACTGG 4 cut(s) 232, 614, 758, 1082
BseRI GAGGAG 6 cut(s) 100, 1655, 2412, 2541, 2664, 2766
BseSI GKGCMC 2 cut(s) 780, 3076
BseXI GCAGC 9 cut(s) 1023, 1198, 1975, 2719, 2815, 2917, 2963, 2969, 3160
BseYI CCCAGC 2 cut(s) 864, 2565
BsgI GTGCAG 2 cut(s) 1065, 2607
Bsh1236I CGCG 2 cut(s) 56, 1795
Bsh1285I CGRYCG 1 cut(s) 29
BshNI GGYRCC 1 cut(s) 908
BshVI ATCGAT 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 5 cut(s) 191, 653, 1063, 1793, 3076
BsiHKCI CYCGRG 1 cut(s) 1379
BsiSI CCGG 6 cut(s) 50, 84, 167, 319, 902, 3087
BslFI GGGAC 8 cut(s) 88, 458, 592, 608, 881, 1348, 2777, 3170
BsmAI GTCTC 4 cut(s) 243, 726, 3044, 3322
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 8 cut(s) 88, 458, 592, 608, 881, 1348, 2777, 3170
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 1379
Bsp1286I GDGCHC 7 cut(s) 191, 261, 653, 780, 1063, 1793, 3076
Bsp13I TCCGGA 1 cut(s) 49
BspDI ATCGAT 1 cut(s) 25
BspEI TCCGGA 1 cut(s) 49
BspFNI CGCG 2 cut(s) 56, 1795
BspLI GGNNCC 7 cut(s) 302, 596, 910, 1889, 2416, 2604, 2886
BspMAI CTGCAG 4 cut(s) 463, 883, 2650, 2907
BspMI ACCTGC 3 cut(s) 2374, 2566, 2590
BspPI GGATC 7 cut(s) 102, 238, 310, 1646, 1806, 2088, 2703
BspQI GCTCTTC 1 cut(s) 1923
BspT107I GGYRCC 1 cut(s) 908
BspTI CTTAAG 3 cut(s) 1808, 1832, 2296
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 3 cut(s) 261, 804, 1854
BsrFI RCCGGY 1 cut(s) 901
BsrI ACTGG 4 cut(s) 232, 614, 758, 1082
BssAI RCCGGY 1 cut(s) 901
BssECI CCNNGG 7 cut(s) 507, 508, 843, 1380, 2145, 2756, 2946
BssT1I CCWWGG 2 cut(s) 843, 2145
Bst6I CTCTTC 3 cut(s) 1923, 2518, 2784
BstAFI CTTAAG 3 cut(s) 1808, 1832, 2296
BstAPI GCANNNNNTGC 1 cut(s) 3137
BstBAI YACGTR 1 cut(s) 3077
BstC8I GCNNGC 8 cut(s) 7, 34, 408, 903, 907, 1793, 2646, 3288
BstDSI CCRYGG 1 cut(s) 2946
BstENI CCTNNNNNAGG 2 cut(s) 2111, 2844
BstF5I GGATG 8 cut(s) 560, 1196, 1291, 1597, 2174, 2561, 3274, 3309
BstFNI CGCG 2 cut(s) 56, 1795
BstH2I RGCGCY 1 cut(s) 1081
BstHHI GCGC 2 cut(s) 58, 1080
BstMAI GTCTC 4 cut(s) 243, 726, 3044, 3322
BstMCI CGRYCG 1 cut(s) 29
BstNSI RCATGY 4 cut(s) 840, 1682, 2792, 2822
BstPAI GACNNNNGTC 1 cut(s) 2135
BstSFI CTRYAG 5 cut(s) 459, 879, 2049, 2646, 2903
BstSLI GKGCMC 2 cut(s) 780, 3076
BstUI CGCG 2 cut(s) 56, 1795
BstV1I GCAGC 9 cut(s) 1023, 1198, 1975, 2719, 2815, 2917, 2963, 2969, 3160
BstV2I GAAGAC 2 cut(s) 649, 3114
BstX2I RGATCY 4 cut(s) 94, 126, 1408, 2093
BstYI RGATCY 4 cut(s) 94, 126, 1408, 2093
Bsu15I ATCGAT 1 cut(s) 25
BsuI GTATCC 4 cut(s) 948, 1438, 2525, 2742
BsuTUI ATCGAT 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 2946
BtsCI GGATG 8 cut(s) 560, 1196, 1291, 1597, 2174, 2561, 3274, 3309
BtsI GCAGTG 3 cut(s) 96, 668, 2900
BtsIMutI CAGTG 7 cut(s) 96, 225, 621, 668, 2448, 2900, 3157
BveI ACCTGC 3 cut(s) 2374, 2566, 2590
Cac8I GCNNGC 8 cut(s) 7, 34, 408, 903, 907, 1793, 2646, 3288
CaiI CAGNNNCTG 4 cut(s) 313, 878, 1601, 2762
CfoI GCGC 2 cut(s) 58, 1080
Cfr10I RCCGGY 1 cut(s) 901
ClaI ATCGAT 1 cut(s) 25
CseI GACGC 2 cut(s) 62, 1923
Csp6I GTAC 4 cut(s) 61, 947, 2248, 3225
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviQI GTAC 4 cut(s) 61, 947, 2248, 3225
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 3 cut(s) 1923, 2518, 2784
EarI CTCTTC 3 cut(s) 1923, 2518, 2784
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 2 cut(s) 189, 1061
Eco130I CCWWGG 2 cut(s) 843, 2145
Eco147I AGGCCT 2 cut(s) 1234, 2226
Eco24I GRGCYC 3 cut(s) 191, 261, 1063
Eco31I GGTCTC 1 cut(s) 243
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 5 cut(s) 544, 595, 1049, 1950, 2415
Eco53kI GAGCTC 2 cut(s) 189, 1061
Eco57I CTGAAG 2 cut(s) 88, 684
Eco72I CACGTG 1 cut(s) 3077
Eco88I CYCGRG 1 cut(s) 1379
EcoICRI GAGCTC 2 cut(s) 189, 1061
EcoNI CCTNNNNNAGG 2 cut(s) 2111, 2844
EcoO109I RGGNCCY 6 cut(s) 595, 1049, 1887, 2415, 2759, 2885
EcoRI GAATTC 1 cut(s) 245
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 843, 2145
EcoT22I ATGCAT 1 cut(s) 1724
EcoT38I GRGCYC 3 cut(s) 191, 261, 1063
ErhI CCWWGG 2 cut(s) 843, 2145
Esp3I CGTCTC 1 cut(s) 726
FalI AAGNNNNNCTT 2 cut(s) 1896, 1928
FaqI GGGAC 8 cut(s) 88, 458, 592, 608, 881, 1348, 2777, 3170
FauI CCCGC 2 cut(s) 1788, 2023
FauNDI CATATG 3 cut(s) 440, 2518, 2574
FbaI TGATCA 2 cut(s) 1353, 3306
FblI GTMKAC 1 cut(s) 182
FokI GGATG 8 cut(s) 547, 1183, 1298, 1604, 2161, 2548, 3281, 3316
FriOI GRGCYC 3 cut(s) 191, 261, 1063
FspBI CTAG 8 cut(s) 689, 1433, 1536, 2217, 2403, 2913, 3165, 3258
GlaI GCGC 2 cut(s) 57, 1079
GsaI CCCAGC 2 cut(s) 868, 2569
GsuI CTGGAG 3 cut(s) 972, 2096, 2916
HaeII RGCGCY 1 cut(s) 1081
HapII CCGG 6 cut(s) 50, 84, 167, 319, 902, 3087
HgaI GACGC 2 cut(s) 62, 1923
HhaI GCGC 2 cut(s) 58, 1080
Hin6I GCGC 2 cut(s) 56, 1078
HinP1I GCGC 2 cut(s) 56, 1078
HincII GTYRAC 4 cut(s) 183, 1563, 1879, 2104
HindII GTYRAC 4 cut(s) 183, 1563, 1879, 2104
HindIII AAGCTT 2 cut(s) 134, 3182
HpaII CCGG 6 cut(s) 50, 84, 167, 319, 902, 3087
Hpy166II GTNNAC 9 cut(s) 183, 272, 716, 1563, 1879, 1950, 2104, 2900, 3074
Hpy8I GTNNAC 9 cut(s) 183, 272, 716, 1563, 1879, 1950, 2104, 2900, 3074
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 6 cut(s) 112, 2081, 2117, 2237, 2523, 2676
HpyCH4IV ACGT 1 cut(s) 3076
HpySE526I ACGT 1 cut(s) 3076
HspAI GCGC 2 cut(s) 56, 1078
Kpn2I TCCGGA 1 cut(s) 49
KroI GCCGGC 1 cut(s) 901
KroNI GCCGGC 1 cut(s) 903
Ksp22I TGATCA 2 cut(s) 1353, 3306
LguI GCTCTTC 1 cut(s) 1923
LmnI GCTCC 9 cut(s) 52, 86, 113, 656, 1066, 1088, 2779, 2935, 2965
Lsp1109I GCAGC 9 cut(s) 1023, 1198, 1975, 2719, 2815, 2917, 2963, 2969, 3160
LweI GCATC 9 cut(s) 419, 569, 974, 1100, 1582, 1735, 2159, 2183, 2570
MaeI CTAG 8 cut(s) 689, 1433, 1536, 2217, 2403, 2913, 3165, 3258
MaeII ACGT 1 cut(s) 3076
MaeIII GTNAC 5 cut(s) 753, 1168, 2277, 2335, 2860
MfeI CAATTG 3 cut(s) 390, 913, 2133
MflI RGATCY 4 cut(s) 94, 126, 1408, 2093
MhlI GDGCHC 7 cut(s) 191, 261, 653, 780, 1063, 1793, 3076
MlyI GAGTC 4 cut(s) 188, 267, 304, 1879
MmeI TCCRAC 3 cut(s) 571, 1066, 2767
Mph1103I ATGCAT 1 cut(s) 1724
MroI TCCGGA 1 cut(s) 49
MroNI GCCGGC 1 cut(s) 901
MroXI GAANNNNTTC 3 cut(s) 1398, 1579, 1671
MslI CAYNNNNRTG 2 cut(s) 1767, 2179
MspA1I CMGCKG 1 cut(s) 2954
MspCI CTTAAG 3 cut(s) 1808, 1832, 2296
MspI CCGG 6 cut(s) 50, 84, 167, 319, 902, 3087
MunI CAATTG 3 cut(s) 390, 913, 2133
MvnI CGCG 2 cut(s) 56, 1795
NaeI GCCGGC 1 cut(s) 903
NciI CCSGG 3 cut(s) 167, 320, 3087
NdeI CATATG 3 cut(s) 440, 2518, 2574
NgoMIV GCCGGC 1 cut(s) 901
NlaIV GGNNCC 7 cut(s) 302, 596, 910, 1889, 2416, 2604, 2886
NmuCI GTSAC 3 cut(s) 753, 1168, 2277
NsiI ATGCAT 1 cut(s) 1724
NspI RCATGY 4 cut(s) 840, 1682, 2792, 2822
PasI CCCWGGG 1 cut(s) 508
PceI AGGCCT 2 cut(s) 1234, 2226
PciI ACATGT 3 cut(s) 836, 2788, 2818
PciSI GCTCTTC 1 cut(s) 1923
PdiI GCCGGC 1 cut(s) 903
PdmI GAANNNNTTC 3 cut(s) 1398, 1579, 1671
PfeI GAWTC 8 cut(s) 22, 198, 632, 667, 1189, 1277, 1667, 2509
PflMI CCANNNNNTGG 2 cut(s) 2762, 3205
PfoI TCCNGGA 3 cut(s) 318, 2112, 2869
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 4 cut(s) 187, 267, 304, 1878
PmaCI CACGTG 1 cut(s) 3077
PmlI CACGTG 1 cut(s) 3077
PpsI GAGTC 4 cut(s) 187, 267, 304, 1878
Ppu21I YACGTR 1 cut(s) 3077
PpuMI RGGWCCY 3 cut(s) 595, 1049, 2415
PscI ACATGT 3 cut(s) 836, 2788, 2818
PshAI GACNNNNGTC 1 cut(s) 2135
PshBI ATTAAT 1 cut(s) 2120
PsiI TTATAA 2 cut(s) 996, 1701
Psp124BI GAGCTC 2 cut(s) 191, 1063
Psp5II RGGWCCY 3 cut(s) 595, 1049, 2415
PspCI CACGTG 1 cut(s) 3077
PspFI CCCAGC 2 cut(s) 864, 2565
PspN4I GGNNCC 7 cut(s) 302, 596, 910, 1889, 2416, 2604, 2886
PspPPI RGGWCCY 3 cut(s) 595, 1049, 2415
PstI CTGCAG 4 cut(s) 463, 883, 2650, 2907
PstNI CAGNNNCTG 4 cut(s) 313, 878, 1601, 2762
PsuI RGATCY 4 cut(s) 94, 126, 1408, 2093
PvuI CGATCG 1 cut(s) 29
RsaI GTAC 4 cut(s) 62, 948, 2249, 3226
RsaNI GTAC 4 cut(s) 61, 947, 2248, 3225
RseI CAYNNNNRTG 2 cut(s) 1767, 2179
SacI GAGCTC 2 cut(s) 191, 1063
SalI GTCGAC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 1923
SchI GAGTC 4 cut(s) 188, 267, 304, 1879
SduI GDGCHC 7 cut(s) 191, 261, 653, 780, 1063, 1793, 3076
SfaNI GCATC 9 cut(s) 419, 569, 974, 1100, 1582, 1735, 2159, 2183, 2570
SfcI CTRYAG 5 cut(s) 459, 879, 2049, 2646, 2903
SinI GGWCC 5 cut(s) 544, 595, 1049, 1950, 2415
SmiMI CAYNNNNRTG 2 cut(s) 1767, 2179
SmlI CTYRAG 7 cut(s) 240, 485, 1439, 1808, 1832, 1867, 2296
SmoI CTYRAG 7 cut(s) 240, 485, 1439, 1808, 1832, 1867, 2296
SpeI ACTAGT 1 cut(s) 3257
SseBI AGGCCT 2 cut(s) 1234, 2226
SspI AATATT 2 cut(s) 1000, 2038
SspMI CTAG 8 cut(s) 689, 1433, 1536, 2217, 2403, 2913, 3165, 3258
SstI GAGCTC 2 cut(s) 191, 1063
StuI AGGCCT 2 cut(s) 1234, 2226
StyI CCWWGG 2 cut(s) 843, 2145
TaiI ACGT 1 cut(s) 3079
TaqI TCGA 4 cut(s) 25, 182, 1243, 2938
TauI GCSGC 5 cut(s) 71, 506, 965, 2009, 2957
TfiI GAWTC 8 cut(s) 22, 198, 632, 667, 1189, 1277, 1667, 2509
TscAI CASTG 7 cut(s) 96, 232, 621, 668, 2448, 2907, 3157
TseFI GTSAC 3 cut(s) 753, 1168, 2277
TseI GCWGC 9 cut(s) 1011, 1211, 1988, 2707, 2828, 2905, 2951, 2957, 3173
Tsp45I GTSAC 3 cut(s) 753, 1168, 2277
TspDTI ATGAA 8 cut(s) 510, 558, 720, 960, 1680, 2100, 2505, 2650
TspGWI ACGGA 1 cut(s) 643
TspRI CASTG 7 cut(s) 96, 232, 621, 668, 2448, 2907, 3157
Van91I CCANNNNNTGG 2 cut(s) 2762, 3205
Vha464I CTTAAG 3 cut(s) 1808, 1832, 2296
VneI GTGCAC 1 cut(s) 3072
VpaK11BI GGWCC 5 cut(s) 544, 595, 1049, 1950, 2415
VspI ATTAAT 1 cut(s) 2120
XagI CCTNNNNNAGG 2 cut(s) 2111, 2844
XbaI TCTAGA 1 cut(s) 3164
XceI RCATGY 4 cut(s) 840, 1682, 2792, 2822
XmiI GTMKAC 1 cut(s) 182
XmnI GAANNNNTTC 3 cut(s) 1398, 1579, 1671
XspI CTAG 8 cut(s) 689, 1433, 1536, 2217, 2403, 2913, 3165, 3258
Zsp2I ATGCAT 1 cut(s) 1724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.