FvH4_7g01850

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010028
Physical Location & Seq
Forward (+)
2215840 .. 2226342
10503 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01850.t3

Sequence Viewer

Length: 3339 bp
ATGGCGTGTATAAAAGGGGTGAACAGATCGGCGTCGGTGGCGGTCGCGCCGGACGCGCCGTACATGGCGGCCGGGACGATGGCGGGAGCCGTGGATCTGTCGTTCAGCTCCTCCGCCAACATCGAAATCTTCAAGCTCGATTTACAGTCCGACGATCGTGATCTTCCGGTGGTCGGAGAGTCGACGAGCTCCGAGAGATTCAACAGGTTGTCGTGGTCCAAGCCCGCTGCTGGATCAGGCTCTCAAGACTTCGGCCTTGGCCTCATCGCCGGTGGACTCGTCGATGGGACTATTGATATCTGGAATCCGCTCACTCTGATTCGTTCCAAGACGGGGGAAAATGCTTCAGTTGAGCATCTCACCAGACATAAGGGGCCAGTTCGTGGTCTTGAATTCAATGCAATTACACCACATTTACTTGCATCTGGGGCCGACGATGGTGAAATTTGCATCTGGGACTTGGCTAATCCTACTGAGCCAACTCAGTTTCCGCCTCTTAAGGGAAGTGGCTCTGCTGCCCAGGGTGAGATTTCATTTTTATCTTGGAATAGCAAGGTCCAACATATACTGGCATCCTCTTCATATAATGGGACAACTGTGATTTGGGACCTAAAAAAGCAAAAGCCAGTGATTAGCTTCACGGATTCAGTTAGAAGACGTTGCTCTGTTTTGCAGTGGAATCCTGATATTGCCACTCAGCTTGTTGTTGCTTCAGATGACGACGGTTCACCTTCTCTCAGGCTCTGGGATATGCGTAATATAATGTCACCAGTTAAAGAGTTTGTGGGGCACACTAAAGGTGTAATTGCAATGTCATGGTGTCCTAACGACAGCTCTTATCTGCTAACATGTGCCAAGGATAATCGAACTATCTGTTGGGACACAGTTTCTGCAGAGATTGTATGTGAATTGCCTGCAGGCACCCACTGGAATTTCGATGTGCACTGGTATCCAAAAGTACCTGGAGTGATATCAGCATCTTCATTTGATGGAAAAATTGGAATTTATAATATTGAGGGTTGCAGTAGATATGGTGTGGGGGAGAGCGACTTTGGTGCTGGGCCTCTGAGAGCACCAAAATGGTACAAGCGACCAGCTGGGGCGTCTTTTGGTTTTGGAGGCAAGATCGTGTCCTTTCACCCTAGTTCATCTGGTGCAGGTGCTTCAGAGGTTTATGTGCACAGCTTGGTTACTGAACAAAGTTTGGTGGATCGCTCATCTGAATTTGAAAGTGCAATACAAAATGGGGAGAGGTCTTCACTCAGGGCTTTATGTGACAAGAAGGCACAGGAGTCTGAGACTGCTGATGACCGGGAAACATGGGGATTATTAAGGGTTATGTTTGAAGATGATGGGACTGCAAGGACAAACCTGATCACACATCTTGGTTTCATTGTACCTGAGGAAACAAAAGAAATTGTTGAAGACAATCTATCTGCAGAAGTCAATGCACCTGGGATTGAGGATTCAACAACTGACAAAGCTGGTTTGGGTGATGAGAAGGAAACTACAATTTTTCCTTCTGACAATGGAGAAGATTTCTTTAACAATCTTCCTAGTCCTAAAGCTGACACTCCTCATTCAACCTCTGGTGACAAATTTGTGGTGGGGGACACAGTTCCTATTACAGACCAAGTTCAAGAAGAACATGATGAACTGGAAGAGAGTGCTGATCCATCATTTGATGAATCAGTTCAACATGCTTTAGCTGTAGGAGATTATAAAGGGGCTGTTGCAAAGTGCATGTCAGCAAATAAAATGGCTGATGCTTTAGTTATTGCTCATGCTGGTGGTCCTACATTGTGGGAGAGCACTCGGGATCAATACCTTAAGTTGAGCCATTCACCTTACCTTAAGATTGTTTCTGCAATGGTGAGCAATGATCTATCAAGTCTTGTTAACACCAGACCCCTCAAATTCTGGAAGGAAACACTTGCTGTTCTCTGTAGTTTTTCATCAGTAGAGGCGTGGGAAGACCTGTGTAATATGCTTGCTGCCAGACTTATAGCTGCTGGTAATACCCTCGCAGCAACTATTTGTTATATATGTGCTGGAAATATTGATAAGACAGTAGATATTTGGTCAAGGAATCTGACAACTGATCACGAAGGGAGATCCTATGTTGACCTTCTTCAGGAATTAATGGAGAAGACTATAGTACTTGCCTTGGCAACTGGACAGAAGCGATTTAGTGCATCTTTGTGTAAACTTGTTGAGAAATATGCTGAAATTTTAGCTAGTCAAGGTCTTTTAACAACAGCACTGGAGTACCTGAAACTCTTGGGCACTGATGAATTGCCACCTGAACTTGTGATCTTAAGGGATCGTATTGCTCTTTCTACAGAACCTGAGAAAGTCGCCAAGTTTGAAAACCCTGCACCAGCTAATATATATCCAGAACCAAACCCTTACAGGCCAGGGAACGTTTCATATTATCAGGAACCAACTCCGACACATGTGCAACCTCGTGGTCCTGGCAATACGTATGATGTTTATCCTGAACCTGCGAATCCTCCGTATCATGGATATTCTCCCGCCCCTTTCCACCAGCCTCCGTCACAGCCTCCATCACAGCCTCCTAACATGTTTGTTCCTATCCAAACACCTCAAGTACCAAAGGACAAATTCTATACAACTCCTGCACCTACTCAGCCTCCCGCGACCTTTATTCCTTCGACTCCTCCTGCTTTGTTAAATGGAGAAAAATATCAGCAGGCCAACACCAACTCGCTGGGTTCTCAGTTGTATCCTCAGGGAGGTGCTTTTCAACATATGCAAACAGGGCCTAGTTCAGGAGCTCCTCATCAATCACATGTCAATCCAGTTCCCGGAAATAAGATGCCACAATCTGTGCCCCCTCCACAAAGTGGGTTTATGCCAGTCACTAATCCAGGAGTTGTTCAGGGGACTCTGCAGCCATCTAGTCCCCCTGCTCCAGCTCGACAGTCTGTGGCTCCAGCTCCACCACCTACCATACAGACAGCTGATACTTCAAAAGTTCCTGCCCACCAAAAGCCCATAATCGCAACACTAGGGAGACTTTTCAATGAAACCTCTGAAGCGTTGGGAGGTTCACGTGCGAACCCAGCTAAGAAGCGTGAAATAGAAGATAATTCGAAGAAGATAGGAGCATTGTTTGCCAAACTTAACAGTGGAGACATATCTAAAAATGCGGCTGATAATCTTGTGCAGCTGTGCCAGGCACTGGATAACGGTGATTTCAACACTGCGCTAACAATTCAGATACATCTTACCACTACCGAGTGGGATGAATGCAACTTTTGGCTTGCAACCCTCAAGCGGATGATCAAGACCAGACAAAATGTGAGATTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SRA1 Sec16_C WD40

Protein Analysis

1113

Amino Acids

120.35

Weight (kDa)

5.21

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 88 - 252 1.1e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 89 - 253 8.1e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 89 - 159 2.6e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 89 - 240 2.4e-10 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 89 - 232 2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 129 - 301 1.7e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 173 - 320 1.7e-13 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 174 - 242 9.7e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 176 - 253 7.2e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 198 - 338 3.3e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 220 - 333 1.1e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 245 - 339 3.6e-10 WDR5 beta-propeller domain
TPR_GEMI5 PF23774 556 - 697 1.8e-06 GEMI5 TPR domain
TPR_Sec16 PF12931 565 - 760 5.1e-09 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63460 AT3G63460 AT3G63460
fragaria_vesca FvH4_7g01850 FvH4_7g01850 FvH4_7g01850
malus_domestica MD02G1300000.v1.1 MD07G1024900.v1.1
prunus_persica Prupe.2G024600_v2.0.a1
pyrus_communis pycom02g25200 pycom07g01920
rosa_chinensis RchiOBHm_Chr1g0318481
rosa_laevigata RLG00000030534
rosa_multiflora Rmu_ssc0000021.1_g000001
rosa_roxburghii Rroxscaffold_4G00329170
rosa_rugosa Rorug01G0021600.1
rosa_samantha Rh1AG034000 Rh1BG030000 Rh1CG033500 Rh1DG037100
rosa_wichuraiana Rw1G002700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1008, 1722
AarI CACCTGC 1 cut(s) 1148
Acc36I ACCTGC 2 cut(s) 1148, 2512
AccB1I GGYRCC 1 cut(s) 920
AccB7I CCANNNNNTGG 3 cut(s) 383, 2867, 3205
AccBSI CCGCTC 1 cut(s) 310
AccI GTMKAC 1 cut(s) 182
AccII CGCG 3 cut(s) 47, 56, 2660
AclI AACGTT 1 cut(s) 2424
AclWI GGATC 7 cut(s) 102, 241, 1218, 1667, 1827, 2109, 2331
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 9 cut(s) 392, 444, 931, 1002, 1223, 1598, 1916, 2229, 2624
AcuI CTGAAG 5 cut(s) 330, 696, 1149, 2117, 3078
AcvI CACGTG 1 cut(s) 3077
AcyI GRCGYC 2 cut(s) 32, 1103
AdeI CACNNNGTG 1 cut(s) 2867
AfaI GTAC 7 cut(s) 62, 960, 1085, 1398, 2160, 2270, 2613
AflII CTTAAG 4 cut(s) 497, 1829, 1853, 2317
AflIII ACRYGT 4 cut(s) 848, 2455, 2583, 2812
AjnI CCWGG 7 cut(s) 519, 961, 1453, 2416, 2473, 2890, 3198
AjuI GAANNNNNNNTTGG 2 cut(s) 859, 891
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
Alw21I GWGCWC 6 cut(s) 191, 945, 1075, 1182, 1814, 2800
Alw26I GTCTC 3 cut(s) 1292, 3031, 3150
Alw44I GTGCAC 2 cut(s) 941, 1178
AlwI GGATC 7 cut(s) 102, 241, 1218, 1667, 1827, 2109, 2331
AlwNI CAGNNNCTG 4 cut(s) 744, 890, 2348, 3205
Ama87I CYCGRG 1 cut(s) 1815
AoxI GGCC 9 cut(s) 69, 253, 259, 374, 429, 1061, 2414, 2715, 2783
ApaLI GTGCAC 2 cut(s) 941, 1178
ApeKI GCWGC 7 cut(s) 227, 515, 1994, 2009, 2027, 2914, 3190
ApoI RAATTY 9 cut(s) 392, 444, 931, 1002, 1223, 1598, 1916, 2229, 2624
ArsI GACNNNNNNTTYG 2 cut(s) 1730, 1762
AseI ATTAAT 1 cut(s) 2141
Asp700I GAANNNNTTC 1 cut(s) 1692
AspLEI GCGC 3 cut(s) 49, 58, 3232
AspS9I GGNCC 9 cut(s) 216, 374, 429, 556, 607, 1061, 1793, 2471, 2783
AsuC2I CCSGG 3 cut(s) 73, 1313, 2829
AsuII TTCGAA 1 cut(s) 3116
AvaI CYCGRG 1 cut(s) 1815
AvaII GGWCC 5 cut(s) 216, 556, 607, 1793, 2471
AxyI CCTNAGG 2 cut(s) 1401, 2751
BaeGI GKGCMC 5 cut(s) 792, 945, 1182, 2288, 2856
BaeI ACNNNNGTAYC 2 cut(s) 2252, 2285
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 2 cut(s) 191, 2800
BauI CACGAG 1 cut(s) 2466
BbrPI CACGTG 1 cut(s) 3077
BbsI GAAGAC 5 cut(s) 661, 1248, 1431, 1980, 2156
Bbv12I GWGCWC 6 cut(s) 191, 945, 1075, 1182, 1814, 2800
BbvI GCAGC 7 cut(s) 214, 502, 1981, 1996, 2039, 2926, 3202
BccI CCATC 8 cut(s) 73, 278, 431, 983, 1346, 1684, 2575, 2926
BceAI ACGGC 2 cut(s) 43, 74
BcgI CGANNNNNNTGC 4 cut(s) 1037, 1071, 2440, 2474
BciT130I CCWGG 7 cut(s) 521, 963, 1455, 2418, 2475, 2892, 3200
BciVI GTATCC 2 cut(s) 960, 2757
BclI TGATCA 3 cut(s) 1374, 2101, 3306
BcnI CCSGG 3 cut(s) 73, 1313, 2829
BcoDI GTCTC 3 cut(s) 1292, 3031, 3150
BfaI CTAG 6 cut(s) 1143, 1557, 2238, 2787, 2922, 3032
BfmI CTRYAG 8 cut(s) 891, 915, 1437, 1710, 1945, 2154, 2340, 2912
BfrI CTTAAG 4 cut(s) 497, 1829, 1853, 2317
BfuAI ACCTGC 2 cut(s) 1148, 2512
BfuI GTATCC 2 cut(s) 960, 2757
BisI GCNGC 9 cut(s) 69, 228, 516, 1995, 2010, 2028, 2915, 3174, 3191
BlsI GCNGC 9 cut(s) 70, 229, 517, 1996, 2011, 2029, 2916, 3175, 3192
BmcAI AGTACT 1 cut(s) 2160
Bme18I GGWCC 5 cut(s) 216, 556, 607, 1793, 2471
BmeT110I CYCGRG 1 cut(s) 1815
BmgT120I GGNCC 9 cut(s) 216, 374, 429, 556, 607, 1061, 1793, 2471, 2783
BmiI GGNNCC 7 cut(s) 88, 375, 430, 608, 922, 2442, 2955
BmsI GCATC 8 cut(s) 364, 431, 459, 581, 986, 1756, 2204, 2829
BpiI GAAGAC 5 cut(s) 661, 1248, 1431, 1980, 2156
BpmI CTGGAG 4 cut(s) 984, 2285, 2919, 2940
Bpu14I TTCGAA 1 cut(s) 3116
BpuEI CTTGAG 3 cut(s) 228, 2592, 3281
BpuMI CCSGG 3 cut(s) 73, 1313, 2829
BsaAI YACGTR 2 cut(s) 2484, 3077
BsaBI GATNNNNATC 2 cut(s) 159, 2493
BsaHI GRCGYC 2 cut(s) 32, 1103
BsaJI CCNNGG 8 cut(s) 90, 256, 519, 520, 855, 1454, 2166, 2417
BsaWI WCCGGW 1 cut(s) 166
BsaXI ACNNNNNCTCC 2 cut(s) 2638, 2668
Bse118I RCCGGY 1 cut(s) 269
Bse21I CCTNAGG 2 cut(s) 1401, 2751
Bse3DI GCAATG 3 cut(s) 816, 1875, 1885
Bse8I GATNNNNATC 2 cut(s) 159, 2493
BseBI CCWGG 7 cut(s) 521, 963, 1455, 2418, 2475, 2892, 3200
BseDI CCNNGG 8 cut(s) 90, 256, 519, 520, 855, 1454, 2166, 2417
BseGI GGATG 3 cut(s) 572, 3274, 3309
BseJI GATNNNNATC 2 cut(s) 159, 2493
BseMI GCAATG 3 cut(s) 816, 1875, 1885
BseRI GAGGAG 4 cut(s) 100, 1566, 2670, 2790
BseSI GKGCMC 5 cut(s) 792, 945, 1182, 2288, 2856
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 7 cut(s) 214, 502, 1981, 1996, 2039, 2926, 3202
BseYI CCCAGC 4 cut(s) 1058, 1097, 2731, 3085
BsgI GTGCAG 4 cut(s) 1176, 2361, 2625, 3209
Bsh1236I CGCG 3 cut(s) 47, 56, 2660
Bsh1285I CGRYCG 3 cut(s) 45, 72, 157
BshFI GGCC 9 cut(s) 71, 255, 261, 376, 431, 1063, 2416, 2717, 2785
BshNI GGYRCC 1 cut(s) 920
BsiEI CGRYCG 3 cut(s) 45, 72, 157
BsiHKAI GWGCWC 6 cut(s) 191, 945, 1075, 1182, 1814, 2800
BsiHKCI CYCGRG 1 cut(s) 1815
BsiSI CCGG 6 cut(s) 50, 72, 167, 270, 1312, 2829
BsmAI GTCTC 3 cut(s) 1292, 3031, 3150
BsmI GAATGC 1 cut(s) 3278
BsnI GGCC 9 cut(s) 71, 255, 261, 376, 431, 1063, 2416, 2717, 2785
BsoBI CYCGRG 1 cut(s) 1815
Bsp119I TTCGAA 1 cut(s) 3116
Bsp1286I GDGCHC 9 cut(s) 191, 792, 945, 1075, 1182, 1814, 2288, 2800, 2856
BspANI GGCC 9 cut(s) 71, 255, 261, 376, 431, 1063, 2416, 2717, 2785
BspFNI CGCG 3 cut(s) 47, 56, 2660
BspLI GGNNCC 7 cut(s) 88, 375, 430, 608, 922, 2442, 2955
BspMAI CTGCAG 4 cut(s) 895, 919, 1441, 2916
BspMI ACCTGC 2 cut(s) 1148, 2512
BspPI GGATC 7 cut(s) 102, 241, 1218, 1667, 1827, 2109, 2331
BspT104I TTCGAA 1 cut(s) 3116
BspT107I GGYRCC 1 cut(s) 920
BspTI CTTAAG 4 cut(s) 497, 1829, 1853, 2317
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 3 cut(s) 816, 1875, 1885
BsrFI RCCGGY 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 8 cut(s) 90, 256, 519, 520, 855, 1454, 2166, 2417
BssNI GRCGYC 2 cut(s) 32, 1103
BssSI CACGAG 1 cut(s) 2466
BssT1I CCWWGG 3 cut(s) 256, 855, 2166
Bst2BI CACGAG 1 cut(s) 2466
Bst2UI CCWGG 7 cut(s) 521, 963, 1455, 2418, 2475, 2892, 3200
Bst4CI ACNGT 9 cut(s) 147, 598, 725, 886, 1618, 2071, 2946, 3152, 3215
Bst6I CTCTTC 2 cut(s) 583, 1656
BstACI GRCGYC 2 cut(s) 32, 1103
BstAFI CTTAAG 4 cut(s) 497, 1829, 1853, 2317
BstAPI GCANNNNNTGC 1 cut(s) 3137
BstBAI YACGTR 2 cut(s) 2484, 3077
BstBI TTCGAA 1 cut(s) 3116
BstC8I GCNNGC 6 cut(s) 225, 915, 919, 1992, 2715, 3288
BstDSI CCRYGG 1 cut(s) 90
BstENI CCTNNNNNAGG 3 cut(s) 2132, 2754, 2790
BstF5I GGATG 3 cut(s) 572, 3274, 3309
BstFNI CGCG 3 cut(s) 47, 56, 2660
BstHHI GCGC 3 cut(s) 49, 58, 3232
BstMAI GTCTC 3 cut(s) 1292, 3031, 3150
BstMCI CGRYCG 3 cut(s) 45, 72, 157
BstMWI GCNNNNNNNGC 7 cut(s) 38, 53, 55, 428, 2782, 3086, 3137
BstNI CCWGG 7 cut(s) 521, 963, 1455, 2418, 2475, 2892, 3200
BstNSI RCATGY 6 cut(s) 852, 1703, 1747, 2459, 2587, 2816
BstSFI CTRYAG 8 cut(s) 891, 915, 1437, 1710, 1945, 2154, 2340, 2912
BstSLI GKGCMC 5 cut(s) 792, 945, 1182, 2288, 2856
BstSNI TACGTA 1 cut(s) 2484
BstUI CGCG 3 cut(s) 47, 56, 2660
BstV1I GCAGC 7 cut(s) 214, 502, 1981, 1996, 2039, 2926, 3202
BstV2I GAAGAC 5 cut(s) 661, 1248, 1431, 1980, 2156
BstX2I RGATCY 2 cut(s) 94, 2114
BstXI CCANNNNNNTGG 1 cut(s) 2731
BstYI RGATCY 2 cut(s) 94, 2114
BstZI CGGCCG 1 cut(s) 69
Bsu36I CCTNAGG 2 cut(s) 1401, 2751
BsuI GTATCC 2 cut(s) 960, 2757
BsuRI GGCC 9 cut(s) 71, 255, 261, 376, 431, 1063, 2416, 2717, 2785
BtgI CCRYGG 1 cut(s) 90
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 3 cut(s) 572, 3274, 3309
BtsI GCAGTG 2 cut(s) 680, 3225
BtsIMutI CAGTG 9 cut(s) 633, 680, 925, 943, 2261, 2286, 3157, 3203, 3225
BveI ACCTGC 2 cut(s) 1148, 2512
Cac8I GCNNGC 6 cut(s) 225, 915, 919, 1992, 2715, 3288
CaiI CAGNNNCTG 4 cut(s) 744, 890, 2348, 3205
CfoI GCGC 3 cut(s) 49, 58, 3232
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 9 cut(s) 216, 374, 429, 556, 607, 1061, 1793, 2471, 2783
CseI GACGC 3 cut(s) 21, 62, 1092
Csp6I GTAC 7 cut(s) 61, 959, 1084, 1397, 2159, 2269, 2612
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviQI GTAC 7 cut(s) 61, 959, 1084, 1397, 2159, 2269, 2612
DraIII CACNNNGTG 1 cut(s) 2867
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 583, 1656
EarI CTCTTC 2 cut(s) 583, 1656
EciI GGCGGA 2 cut(s) 103, 480
Ecl136II GAGCTC 2 cut(s) 189, 2798
EclXI CGGCCG 1 cut(s) 69
Eco105I TACGTA 1 cut(s) 2484
Eco130I CCWWGG 3 cut(s) 256, 855, 2166
Eco24I GRGCYC 2 cut(s) 191, 2800
Eco32I GATATC 2 cut(s) 298, 972
Eco47I GGWCC 5 cut(s) 216, 556, 607, 1793, 2471
Eco52I CGGCCG 1 cut(s) 69
Eco53kI GAGCTC 2 cut(s) 189, 2798
Eco57I CTGAAG 5 cut(s) 330, 696, 1149, 2117, 3078
Eco72I CACGTG 1 cut(s) 3077
Eco81I CCTNAGG 2 cut(s) 1401, 2751
Eco88I CYCGRG 1 cut(s) 1815
EcoICRI GAGCTC 2 cut(s) 189, 2798
EcoNI CCTNNNNNAGG 3 cut(s) 2132, 2754, 2790
EcoO109I RGGNCCY 2 cut(s) 607, 2783
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 7 cut(s) 519, 961, 1453, 2416, 2473, 2890, 3198
EcoRV GATATC 2 cut(s) 298, 972
EcoT14I CCWWGG 3 cut(s) 256, 855, 2166
EcoT38I GRGCYC 2 cut(s) 191, 2800
ErhI CCWWGG 3 cut(s) 256, 855, 2166
FauI CCCGC 4 cut(s) 76, 232, 2542, 2665
FauNDI CATATG 1 cut(s) 2772
FbaI TGATCA 3 cut(s) 1374, 2101, 3306
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 9 cut(s) 69, 228, 516, 1995, 2010, 2028, 2915, 3174, 3191
FokI GGATG 3 cut(s) 559, 3281, 3316
FriOI GRGCYC 2 cut(s) 191, 2800
Fsp4HI GCNGC 9 cut(s) 69, 228, 516, 1995, 2010, 2028, 2915, 3174, 3191
FspBI CTAG 6 cut(s) 1143, 1557, 2238, 2787, 2922, 3032
GlaI GCGC 3 cut(s) 48, 57, 3231
GluI GCNGC 9 cut(s) 69, 228, 516, 1995, 2010, 2028, 2915, 3174, 3191
GsaI CCCAGC 4 cut(s) 1062, 1101, 2735, 3089
GsuI CTGGAG 4 cut(s) 984, 2285, 2919, 2940
HaeIII GGCC 9 cut(s) 71, 255, 261, 376, 431, 1063, 2416, 2717, 2785
HapII CCGG 6 cut(s) 50, 72, 167, 270, 1312, 2829
HgaI GACGC 3 cut(s) 21, 62, 1092
HhaI GCGC 3 cut(s) 49, 58, 3232
Hin1I GRCGYC 2 cut(s) 32, 1103
Hin6I GCGC 3 cut(s) 47, 56, 3230
HinP1I GCGC 3 cut(s) 47, 56, 3230
HincII GTYRAC 3 cut(s) 183, 1900, 2125
HindII GTYRAC 3 cut(s) 183, 1900, 2125
HpaI GTTAAC 1 cut(s) 1900
HpaII CCGG 6 cut(s) 50, 72, 167, 270, 1312, 2829
Hpy99I CGWCG 6 cut(s) 37, 155, 187, 284, 437, 725
HpyAV CCTTC 8 cut(s) 741, 1276, 1495, 1530, 1918, 2102, 2138, 2682
HpyCH4III ACNGT 9 cut(s) 147, 598, 725, 886, 1618, 2071, 2946, 3152, 3215
HpyCH4IV ACGT 4 cut(s) 658, 2424, 2483, 3076
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 53, 55, 428, 2782, 3086, 3137
HpySE526I ACGT 4 cut(s) 658, 2424, 2483, 3076
Hsp92I GRCGYC 2 cut(s) 32, 1103
HspAI GCGC 3 cut(s) 47, 56, 3230
Ksp22I TGATCA 3 cut(s) 1374, 2101, 3306
KspAI GTTAAC 1 cut(s) 1900
LmnI GCTCC 9 cut(s) 86, 113, 194, 2795, 2803, 2938, 2959, 2965, 3128
Lsp1109I GCAGC 7 cut(s) 214, 502, 1981, 1996, 2039, 2926, 3202
LweI GCATC 8 cut(s) 364, 431, 459, 581, 986, 1756, 2204, 2829
MaeI CTAG 6 cut(s) 1143, 1557, 2238, 2787, 2922, 3032
MaeII ACGT 4 cut(s) 658, 2424, 2483, 3076
MaeIII GTNAC 6 cut(s) 765, 1189, 1274, 1592, 2556, 2881
MbiI CCGCTC 1 cut(s) 310
MflI RGATCY 2 cut(s) 94, 2114
MhlI GDGCHC 9 cut(s) 191, 792, 945, 1075, 1182, 1814, 2288, 2800, 2856
MlyI GAGTC 5 cut(s) 188, 270, 1301, 2671, 2902
MmeI TCCRAC 4 cut(s) 154, 174, 583, 2474
MroXI GAANNNNTTC 1 cut(s) 1692
MslI CAYNNNNRTG 3 cut(s) 1078, 1788, 2200
MspA1I CMGCKG 4 cut(s) 227, 1097, 2984, 3193
MspCI CTTAAG 4 cut(s) 497, 1829, 1853, 2317
MspI CCGG 6 cut(s) 50, 72, 167, 270, 1312, 2829
Mva1269I GAATGC 1 cut(s) 3278
MvaI CCWGG 7 cut(s) 521, 963, 1455, 2418, 2475, 2892, 3200
MvnI CGCG 3 cut(s) 47, 56, 2660
MwoI GCNNNNNNNGC 7 cut(s) 38, 53, 55, 428, 2782, 3086, 3137
NciI CCSGG 3 cut(s) 73, 1313, 2829
NdeI CATATG 1 cut(s) 2772
NlaIV GGNNCC 7 cut(s) 88, 375, 430, 608, 922, 2442, 2955
NmuCI GTSAC 5 cut(s) 765, 1274, 1592, 2556, 2881
NspI RCATGY 6 cut(s) 852, 1703, 1747, 2459, 2587, 2816
NspV TTCGAA 1 cut(s) 3116
PaqCI CACCTGC 1 cut(s) 1148
PasI CCCWGGG 1 cut(s) 520
PciI ACATGT 4 cut(s) 848, 2455, 2583, 2812
PctI GAATGC 1 cut(s) 3278
PdmI GAANNNNTTC 1 cut(s) 1692
PfeI GAWTC 9 cut(s) 198, 304, 319, 644, 679, 1466, 1688, 2089, 2509
PflMI CCANNNNNTGG 3 cut(s) 383, 2867, 3205
PfoI TCCNGGA 2 cut(s) 2827, 2890
PkrI GCNGC 9 cut(s) 70, 229, 517, 1996, 2011, 2029, 2916, 3175, 3192
Ple19I CGATCG 1 cut(s) 157
PleI GAGTC 5 cut(s) 187, 270, 1300, 2671, 2902
PmaCI CACGTG 1 cut(s) 3077
PmlI CACGTG 1 cut(s) 3077
PpsI GAGTC 5 cut(s) 187, 270, 1300, 2671, 2902
Ppu21I YACGTR 2 cut(s) 2484, 3077
PpuMI RGGWCCY 1 cut(s) 607
PscI ACATGT 4 cut(s) 848, 2455, 2583, 2812
PshBI ATTAAT 1 cut(s) 2141
PsiI TTATAA 2 cut(s) 1008, 1722
Psp124BI GAGCTC 2 cut(s) 191, 2800
Psp1406I AACGTT 1 cut(s) 2424
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 7 cut(s) 519, 961, 1453, 2416, 2473, 2890, 3198
PspCI CACGTG 1 cut(s) 3077
PspFI CCCAGC 4 cut(s) 1058, 1097, 2731, 3085
PspGI CCWGG 7 cut(s) 519, 961, 1453, 2416, 2473, 2890, 3198
PspN4I GGNNCC 7 cut(s) 88, 375, 430, 608, 922, 2442, 2955
PspPI GGNCC 9 cut(s) 216, 374, 429, 556, 607, 1061, 1793, 2471, 2783
PspPPI RGGWCCY 1 cut(s) 607
PstI CTGCAG 4 cut(s) 895, 919, 1441, 2916
PstNI CAGNNNCTG 4 cut(s) 744, 890, 2348, 3205
PsuI RGATCY 2 cut(s) 94, 2114
PvuI CGATCG 1 cut(s) 157
PvuII CAGCTG 3 cut(s) 1097, 2984, 3193
RsaI GTAC 7 cut(s) 62, 960, 1085, 1398, 2160, 2270, 2613
RsaNI GTAC 7 cut(s) 61, 959, 1084, 1397, 2159, 2269, 2612
RseI CAYNNNNRTG 3 cut(s) 1078, 1788, 2200
SacI GAGCTC 2 cut(s) 191, 2800
SalI GTCGAC 1 cut(s) 181
SatI GCNGC 9 cut(s) 69, 228, 516, 1995, 2010, 2028, 2915, 3174, 3191
Sau96I GGNCC 9 cut(s) 216, 374, 429, 556, 607, 1061, 1793, 2471, 2783
SbfI CCTGCAGG 1 cut(s) 919
ScaI AGTACT 1 cut(s) 2160
SchI GAGTC 5 cut(s) 188, 270, 1301, 2671, 2902
SdaI CCTGCAGG 1 cut(s) 919
SduI GDGCHC 9 cut(s) 191, 792, 945, 1075, 1182, 1814, 2288, 2800, 2856
SfaNI GCATC 8 cut(s) 364, 431, 459, 581, 986, 1756, 2204, 2829
SfcI CTRYAG 8 cut(s) 891, 915, 1437, 1710, 1945, 2154, 2340, 2912
SfuI TTCGAA 1 cut(s) 3116
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 5 cut(s) 216, 556, 607, 1793, 2471
SmiMI CAYNNNNRTG 3 cut(s) 1078, 1788, 2200
SmlI CTYRAG 7 cut(s) 243, 497, 1829, 1853, 2317, 2607, 3296
SmoI CTYRAG 7 cut(s) 243, 497, 1829, 1853, 2317, 2607, 3296
SnaBI TACGTA 1 cut(s) 2484
Sse8387I CCTGCAGG 1 cut(s) 919
SspI AATATT 2 cut(s) 1012, 2059
SspMI CTAG 6 cut(s) 1143, 1557, 2238, 2787, 2922, 3032
SstI GAGCTC 2 cut(s) 191, 2800
StyI CCWWGG 3 cut(s) 256, 855, 2166
TaaI ACNGT 9 cut(s) 147, 598, 725, 886, 1618, 2071, 2946, 3152, 3215
TaiI ACGT 4 cut(s) 661, 2427, 2486, 3079
TaqI TCGA 9 cut(s) 123, 138, 182, 282, 865, 936, 2675, 2941, 3116
TatI WGTACW 1 cut(s) 2158
TauI GCSGC 2 cut(s) 71, 3176
TfiI GAWTC 9 cut(s) 198, 304, 319, 644, 679, 1466, 1688, 2089, 2509
TscAI CASTG 9 cut(s) 633, 680, 932, 950, 2268, 2293, 3157, 3210, 3232
TseFI GTSAC 5 cut(s) 765, 1274, 1592, 2556, 2881
TseI GCWGC 7 cut(s) 227, 515, 1994, 2009, 2027, 2914, 3190
Tsp45I GTSAC 5 cut(s) 765, 1274, 1592, 2556, 2881
TspGWI ACGGA 3 cut(s) 656, 2505, 2544
TspRI CASTG 9 cut(s) 633, 680, 932, 950, 2268, 2293, 3157, 3210, 3232
Van91I CCANNNNNTGG 3 cut(s) 383, 2867, 3205
Vha464I CTTAAG 4 cut(s) 497, 1829, 1853, 2317
VneI GTGCAC 2 cut(s) 941, 1178
VpaK11BI GGWCC 5 cut(s) 216, 556, 607, 1793, 2471
VspI ATTAAT 1 cut(s) 2141
XagI CCTNNNNNAGG 3 cut(s) 2132, 2754, 2790
XapI RAATTY 9 cut(s) 392, 444, 931, 1002, 1223, 1598, 1916, 2229, 2624
XceI RCATGY 6 cut(s) 852, 1703, 1747, 2459, 2587, 2816
XmiI GTMKAC 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 1692
XspI CTAG 6 cut(s) 1143, 1557, 2238, 2787, 2922, 3032
ZrmI AGTACT 1 cut(s) 2160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.