Rroxscaffold_4G00329170

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010028
Physical Location & Seq
Reverse (-)
62374401 .. 62385256
10856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329170.1

Sequence Viewer

Length: 3300 bp
ATGGCGTGTATAAAGGGGGTGAACAGATCAGCGTCGGTGGCGGTCGCGCCGGACGCTCCGTACATGGCGGCCGGGACGATGGCGGGAGCGGTGGATCTGTCCTTCAGCTCCTCAGCGAATATCGAGATCTTCAAGCTCGATTTACAGTCGGACGATCGTGATCTTCCTGTAGTGGGAGAGTCGACGAGCTCCGAGAGATTCAACAGGTTATCGTGGTCCAAGCCCGCTGCTGGATCAGGCTCGCAAGACTTTGGCCTTGGGCTCATCGCCGGTGGACTCGTCGATGGGACTATTGATATCTGGAATCCGCTCACTCTGATCCGTTCTGAGACGGGGGAAAATGCTTCTGTTGAGCGTCTGACAAGACATAAGGGGCCTGTTCGTGGTCTTGAATTTAATGCAATCGCTCCAAATTTACTTGCATCTGGGGCCGACGATGGTGAAATTTGCATCTGGGACTTGGCTAATCCTACTGAGCCAACTCATTTTCCGCCTCTTAGGGGAAGTGGTTCTGCTGCCCAGGGTGAGATTTCATTTTTATCTTGGAATAGCAAGGTCCAACATATATTGGCATCCTCTTCGTATAATGGGACAACTGTGATTTGGGACCTAAAAAAGCAAAAGCCAGTGATAAGTTTTGCGGATTCAGTTAGAAGACGGTGCTCTGTTTTGCAGTGGAATCCTGATGTTGCCACTCAGCTTGTTGTTGCATCTGATGAAGATGGTTCACCTTCTCTCAGGCTATGGGATATGCGTAATATATTGTCACCAGTTAAAGAGTTTGTGGGGCACACTAAAGGTGTAATTGCAATGTCATGGTGTCCTAATGACAGCTCTTATCTGCTAACGTGTGCCAAGGACAATCGAACTATTTGTTGGGACACAGTTTCTGCAGAGATTGTATGTGAATTGCCTGCAGGCACCAACTGGAATTTTGATGTGCATTGGTATCCGAAAGTACCTGGAGTGATATCAGCGTCTTCATTTGATGGAAAAATTGGAATTTATAATATTGAGGGTTGCAGTAGATATGGTGTGGGGGAGAGCGACTTTGGTGCTGGACCTCTCAGAGCTCCAAAATGGTATAAGCGCCCAGCTGGAGCATCTTTTGGTTTCGGAGGCAAGATTGTGTCCTTTCACCCTAGTTCATCTGGTGTTTCAGAGGTTTATGTGCACAGCTTGGTTACTGAACAAAGTTTGGTTGATCGCTCATCTGAATTTGAAAGTGCAATACAAAATGGGGAAAGGTCTTCACTCCGGGCTTTATGTGACAAGAAAGCACAGGAGTCTGAGTCTGCGGATGACCGGGAAACATGGGGCTTGTTAAGGGTTATGTTTGAAGATGATGGGACTGCAAGGACAAACCTGATCACACATCTTGGTTTCAGTGTACCTGAGGAAACAAAAGAAACAGTTCAAGATGGTCTATCAGCGGAAGTCAGTGCACCTGGGATTGAGGATTCAACAACTGATAAAGCTGGTTTGGGTGCTGAGAAGGAAACTACAATTTTTCCTTCTGACAATGGAGAAGATTTCTTTAACAATCTTCCTAGTCCTAAAGCTGACACTCCTCATTCAACCTCTGGTGACAAATTTGTTGCGGCGGATACAGTTCCTATTACAGACCAAGTTCAAGAAGAACCTGATGAACCGGAAGAGAGCGCCGATCCTTCATTTGATGAATCAGTTCAACATGCTTTAGCTGTAGGAGATTATAAAGGGGCTGTTGCAAAGTGCATTTCAGCAAATAAAATGGCTGATGCTTTAGTTATTGCACATGCTGGTGGTCCGACGTTGTGGGAGCGCACTCGGGATCAATACCTTAAGTTGAGCCATTCACCTTACCTTAAGATTGTTTCTGCAATGGTGAGCAATGATCTATCAAGTCTTGTTAACACCAGACCCCTCAAATTCTGGAAGGAAACACTTGCTGTTCTCTGTAGTTTTTCATCAATAGAGGCGTGGGAAGATCTCTGTAATACGCTTGCTGCTAGACTTATAGCTGCTGGTAATACCCTGGCAGCAACTATTTGTTATATATGTGCGGGAAATATTGACAAGACGGTAGATATTTGGTCAAGGAATCTGACAACTGATCATGAAGGGAGATCCTATGTTGACCTTCTTCAGGAATTAATGGAGAAGACTATAGTACTTGCCTTGGCAACTGGACAGAAGCGATTTAGTGTCTCTTTGTGCAAACTTGTTGAGAAATATGCTGAAATTTTAGCTAGTCAAGGTCTTTTAACAACAGCAATGGAGTACCTGAAACTCTTGGGCTCTGATGAATTGTCTCCTGAACTTGTGATCTTAAGGGATCGTATTGCTCTTTCTACAGCACCTGTAGAGAAAGTCCCCAAGACTGTAACATTTGAAAGCCCTGATCAAACCCCTTATGTGGGTGGGAATTCTCCATATTATCAGGAACCAGCTCAGCCACCTGTGAATCCGTATGGCGGCTATTCTGAACCTGTGACTCCTCCGTATGCAGGTGGAGGGTATCCTCATGTCCCATACCCCCAGCCTCCATCACAGACTCCATCACAGCCTCCTCACATGTTTATTCCTACACCAACACCTCAACTACCACAGGAAAAATATCCTGTACCTCCTGCTTCTACTCAGCCTAACGTGTCCTTTATTCCTTCGACTCCTCCTGCTCTGTTAAATGTGGAGAAGTATCAGCAGCCTACGCTGGGTTCTCAATTGTATCCTGGAACTGCTTTTCAACCTATGCAAACAGGGCCTGGTTCAGGAGCCCCTCATCAATCACATGTCAATCCAGTTCCTGCAAGTAAGATGCCTCATGCTGTACCCCCTCCACAAAGGGGATTTATGCCAGTCACTAGTTCAGGAGTTGTTCAGGGGACTCAGCAGCCATCTAGTCCCCCTGCTCCACCTCGACCTTCCGTGGCTCCTGCTCCACCACCTACCATACAGACAGTTGATACTTCAAAAGTTCCCGGCCACCAAAAACCCGTAATCGCAACATTAGGGAGACTTTTCAATGAAACATCTGAAGCATTGGGAGGTTCACGAGCAAATCCTGCTAAGAAGCGTGAAATAGAAGACAATTCAAGGAAGATAGGTGCACTGTTTGCCAAACTCAACAGCGGAGACATATCTAAAAATGCTGGTGATAAACTTGTGCAGCTGTGCCAGGCACTGGATAATGGTGATTTCACCACTGCCCTAGGCATTCAGGTACAACTTACCACTAGTGAGTGGGATGAGTGCAACTTTTGGCTTGCAACTCTCAAGCGGATGATCAAGACCAGGCAAAATATGAGATTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SRA1 Sec16_C WD40

Protein Analysis

1099

Amino Acids

118.46

Weight (kDa)

5.17

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 88 - 240 3.4e-11 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 88 - 252 6e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 89 - 264 6.2e-13 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 89 - 157 5e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 89 - 229 1.4e-09 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 89 - 238 9.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 138 - 301 1.1e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 173 - 320 6.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 198 - 338 7.7e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 219 - 333 8.6e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 245 - 339 7e-10 WDR5 beta-propeller domain
TPR_GEMI5 PF23774 557 - 695 2.1e-06 GEMI5 TPR domain
TPR_Sec16 PF12931 563 - 758 6.1e-09 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63460 AT3G63460 AT3G63460
fragaria_vesca FvH4_7g01850 FvH4_7g01850 FvH4_7g01850
malus_domestica MD02G1300000.v1.1 MD07G1024900.v1.1
prunus_persica Prupe.2G024600_v2.0.a1
pyrus_communis pycom02g25200 pycom07g01920
rosa_chinensis RchiOBHm_Chr1g0318481
rosa_laevigata RLG00000030534
rosa_multiflora Rmu_ssc0000021.1_g000001
rosa_roxburghii Rroxscaffold_4G00329170
rosa_rugosa Rorug01G0021600.1
rosa_samantha Rh1AG034000 Rh1BG030000 Rh1CG033500 Rh1DG037100
rosa_wichuraiana Rw1G002700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1008, 1716
AarI CACCTGC 1 cut(s) 2480
Acc36I ACCTGC 1 cut(s) 2480
AccB1I GGYRCC 1 cut(s) 920
AccB7I CCANNNNNTGG 1 cut(s) 3166
AccBSI CCGCTC 2 cut(s) 89, 310
AccI GTMKAC 1 cut(s) 182
AccII CGCG 1 cut(s) 47
AclWI GGATC 7 cut(s) 102, 241, 313, 1661, 1821, 2103, 2325
AcoI YGGCCR 2 cut(s) 69, 2965
AcuI CTGAAG 3 cut(s) 88, 2111, 3039
AfaI GTAC 8 cut(s) 62, 960, 1392, 2154, 2264, 2607, 2814, 3207
AflII CTTAAG 3 cut(s) 1823, 1847, 2311
AflIII ACRYGT 4 cut(s) 848, 2556, 2631, 2773
AhlI ACTAGT 2 cut(s) 2846, 3218
AjnI CCWGG 8 cut(s) 519, 961, 1447, 2016, 2713, 2746, 3159, 3275
AjuI GAANNNNNNNTTGG 2 cut(s) 859, 891
Alw21I GWGCWC 6 cut(s) 191, 665, 1075, 1176, 1447, 3094
Alw26I GTCTC 5 cut(s) 323, 2194, 2298, 2992, 3111
Alw44I GTGCAC 3 cut(s) 1172, 1443, 3090
AlwI GGATC 7 cut(s) 102, 241, 313, 1661, 1821, 2103, 2325
AlwNI CAGNNNCTG 5 cut(s) 890, 2342, 2747, 2789, 3166
Ama87I CYCGRG 1 cut(s) 1809
AoxI GGCC 6 cut(s) 69, 253, 374, 429, 2744, 2965
ApaLI GTGCAC 3 cut(s) 1172, 1443, 3090
ApeKI GCWGC 8 cut(s) 227, 515, 1988, 2003, 2021, 2686, 2875, 3151
AseI ATTAAT 1 cut(s) 2135
Asp700I GAANNNNTTC 3 cut(s) 508, 1413, 1686
AspA2I CCTAGG 1 cut(s) 3193
AspLEI GCGC 4 cut(s) 49, 1092, 1664, 1806
AspS9I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
AsuC2I CCSGG 4 cut(s) 73, 1259, 1307, 2964
AvaI CYCGRG 1 cut(s) 1809
AvaII GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
AvrII CCTAGG 1 cut(s) 3193
AxyI CCTNAGG 1 cut(s) 1395
BaeGI GKGCMC 4 cut(s) 792, 1176, 1447, 3094
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
BauI CACGAG 1 cut(s) 3036
BbsI GAAGAC 5 cut(s) 661, 972, 1242, 2150, 3075
Bbv12I GWGCWC 6 cut(s) 191, 665, 1075, 1176, 1447, 3094
BbvCI CCTCAGC 1 cut(s) 112
BbvI GCAGC 8 cut(s) 214, 502, 1975, 1990, 2033, 2698, 2887, 3163
BcgI CGANNNNNNTGC 4 cut(s) 561, 595, 1037, 1071
BciT130I CCWGG 8 cut(s) 521, 963, 1449, 2018, 2715, 2748, 3161, 3277
BciVI GTATCC 4 cut(s) 960, 1600, 2511, 2721
BclI TGATCA 4 cut(s) 1368, 2095, 2383, 3267
BcnI CCSGG 4 cut(s) 73, 1259, 1307, 2964
BcoDI GTCTC 5 cut(s) 323, 2194, 2298, 2992, 3111
BcuI ACTAGT 2 cut(s) 2846, 3218
BfaI CTAG 8 cut(s) 1143, 1551, 1992, 2232, 2847, 2883, 3194, 3219
BfmI CTRYAG 8 cut(s) 168, 891, 915, 1704, 1939, 2148, 2334, 2343
BfoI RGCGCY 2 cut(s) 1093, 1665
BfrI CTTAAG 3 cut(s) 1823, 1847, 2311
BfuAI ACCTGC 1 cut(s) 2480
BfuI GTATCC 4 cut(s) 960, 1600, 2511, 2721
BglII AGATCT 2 cut(s) 126, 1969
BlnI CCTAGG 1 cut(s) 3193
BlpI GCTNAGC 1 cut(s) 2433
BmcAI AGTACT 1 cut(s) 2154
Bme18I GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
BmeT110I CYCGRG 1 cut(s) 1809
BmgT120I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
BmiI GGNNCC 7 cut(s) 375, 430, 608, 922, 2427, 2758, 2916
BmsI GCATC 7 cut(s) 431, 459, 581, 719, 1112, 1750, 2790
BpiI GAAGAC 5 cut(s) 661, 972, 1242, 2150, 3075
BpmI CTGGAG 2 cut(s) 984, 1119
Bpu10I CCTNAGC 1 cut(s) 112
Bpu1102I GCTNAGC 1 cut(s) 2433
BpuEI CTTGAG 1 cut(s) 3242
BpuMI CCSGG 4 cut(s) 73, 1259, 1307, 2964
BsaBI GATNNNNATC 1 cut(s) 159
BsaJI CCNNGG 9 cut(s) 256, 519, 520, 855, 1448, 2016, 2160, 2910, 3193
BsaWI WCCGGW 1 cut(s) 1651
BsaXI ACNNNNNCTCC 2 cut(s) 1277, 1307
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 7 cut(s) 626, 770, 932, 2173, 2783, 2840, 3171
Bse21I CCTNAGG 1 cut(s) 1395
Bse3DI GCAATG 4 cut(s) 816, 1869, 1879, 2262
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 8 cut(s) 521, 963, 1449, 2018, 2715, 2748, 3161, 3277
BseDI CCNNGG 9 cut(s) 256, 519, 520, 855, 1448, 2016, 2160, 2910, 3193
BseGI GGATG 4 cut(s) 572, 1306, 3235, 3270
BseJI GATNNNNATC 1 cut(s) 159
BseMI GCAATG 4 cut(s) 816, 1869, 1879, 2262
BseNI ACTGG 7 cut(s) 626, 770, 932, 2173, 2783, 2840, 3171
BseRI GAGGAG 5 cut(s) 100, 1560, 2469, 2541, 2643
BseSI GKGCMC 4 cut(s) 792, 1176, 1447, 3094
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 8 cut(s) 214, 502, 1975, 1990, 2033, 2698, 2887, 3163
BseYI CCCAGC 3 cut(s) 1093, 2520, 2695
BsgI GTGCAG 1 cut(s) 3170
Bsh1236I CGCG 1 cut(s) 47
Bsh1285I CGRYCG 3 cut(s) 45, 72, 157
BshFI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
BshNI GGYRCC 1 cut(s) 920
BsiEI CGRYCG 3 cut(s) 45, 72, 157
BsiHKAI GWGCWC 6 cut(s) 191, 665, 1075, 1176, 1447, 3094
BsiHKCI CYCGRG 1 cut(s) 1809
BsiSI CCGG 7 cut(s) 50, 72, 270, 1258, 1306, 1652, 2964
BsmAI GTCTC 5 cut(s) 323, 2194, 2298, 2992, 3111
BsmBI CGTCTC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 3198
BsnI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
BsoBI CYCGRG 1 cut(s) 1809
Bsp1720I GCTNAGC 1 cut(s) 2433
BspANI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
BspFNI CGCG 1 cut(s) 47
BspHI TCATGA 1 cut(s) 2098
BspLI GGNNCC 7 cut(s) 375, 430, 608, 922, 2427, 2758, 2916
BspMAI CTGCAG 2 cut(s) 895, 919
BspMI ACCTGC 1 cut(s) 2480
BspPI GGATC 7 cut(s) 102, 241, 313, 1661, 1821, 2103, 2325
BspT107I GGYRCC 1 cut(s) 920
BspTI CTTAAG 3 cut(s) 1823, 1847, 2311
BsrBI CCGCTC 2 cut(s) 89, 310
BsrDI GCAATG 4 cut(s) 816, 1869, 1879, 2262
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 7 cut(s) 626, 770, 932, 2173, 2783, 2840, 3171
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 9 cut(s) 256, 519, 520, 855, 1448, 2016, 2160, 2910, 3193
BssSI CACGAG 1 cut(s) 3036
BssT1I CCWWGG 4 cut(s) 256, 855, 2160, 3193
Bst2BI CACGAG 1 cut(s) 3036
Bst2UI CCWGG 8 cut(s) 521, 963, 1449, 2018, 2715, 2748, 3161, 3277
Bst6I CTCTTC 2 cut(s) 583, 1650
BstAFI CTTAAG 3 cut(s) 1823, 1847, 2311
BstAPI GCANNNNNTGC 2 cut(s) 3047, 3098
BstC8I GCNNGC 6 cut(s) 225, 242, 915, 919, 1986, 3249
BstDSI CCRYGG 1 cut(s) 2910
BstENI CCTNNNNNAGG 2 cut(s) 2126, 2751
BstF5I GGATG 4 cut(s) 572, 1306, 3235, 3270
BstFNI CGCG 1 cut(s) 47
BstH2I RGCGCY 2 cut(s) 1093, 1665
BstHHI GCGC 4 cut(s) 49, 1092, 1664, 1806
BstMAI GTCTC 5 cut(s) 323, 2194, 2298, 2992, 3111
BstMCI CGRYCG 3 cut(s) 45, 72, 157
BstMWI GCNNNNNNNGC 7 cut(s) 38, 53, 428, 2692, 2743, 3047, 3098
BstNI CCWGG 8 cut(s) 521, 963, 1449, 2018, 2715, 2748, 3161, 3277
BstNSI RCATGY 4 cut(s) 1697, 1781, 2560, 2777
BstSFI CTRYAG 8 cut(s) 168, 891, 915, 1704, 1939, 2148, 2334, 2343
BstSLI GKGCMC 4 cut(s) 792, 1176, 1447, 3094
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 8 cut(s) 214, 502, 1975, 1990, 2033, 2698, 2887, 3163
BstV2I GAAGAC 5 cut(s) 661, 972, 1242, 2150, 3075
BstX2I RGATCY 4 cut(s) 94, 126, 1969, 2108
BstYI RGATCY 4 cut(s) 94, 126, 1969, 2108
BstZI CGGCCG 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 1395
BsuI GTATCC 4 cut(s) 960, 1600, 2511, 2721
BsuRI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
BtgI CCRYGG 1 cut(s) 2910
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 4 cut(s) 572, 1306, 3235, 3270
BtsI GCAGTG 2 cut(s) 680, 3186
BtsIMutI CAGTG 7 cut(s) 633, 680, 1393, 1447, 3092, 3164, 3186
BveI ACCTGC 1 cut(s) 2480
Cac8I GCNNGC 6 cut(s) 225, 242, 915, 919, 1986, 3249
CaiI CAGNNNCTG 5 cut(s) 890, 2342, 2747, 2789, 3166
CciI TCATGA 1 cut(s) 2098
CfoI GCGC 4 cut(s) 49, 1092, 1664, 1806
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
CseI GACGC 4 cut(s) 21, 62, 344, 966
Csp6I GTAC 8 cut(s) 61, 959, 1391, 2153, 2263, 2606, 2813, 3206
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviQI GTAC 8 cut(s) 61, 959, 1391, 2153, 2263, 2606, 2813, 3206
EaeI YGGCCR 2 cut(s) 69, 2965
EagI CGGCCG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 583, 1650
EarI CTCTTC 2 cut(s) 583, 1650
EciI GGCGGA 2 cut(s) 480, 1619
Ecl136II GAGCTC 2 cut(s) 189, 1073
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 4 cut(s) 256, 855, 2160, 3193
Eco24I GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
Eco32I GATATC 2 cut(s) 298, 972
Eco47I GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
Eco52I CGGCCG 1 cut(s) 69
Eco53kI GAGCTC 2 cut(s) 189, 1073
Eco57I CTGAAG 3 cut(s) 88, 2111, 3039
Eco81I CCTNAGG 1 cut(s) 1395
Eco88I CYCGRG 1 cut(s) 1809
EcoICRI GAGCTC 2 cut(s) 189, 1073
EcoNI CCTNNNNNAGG 2 cut(s) 2126, 2751
EcoO109I RGGNCCY 3 cut(s) 374, 607, 2744
EcoRI GAATTC 1 cut(s) 2407
EcoRII CCWGG 8 cut(s) 519, 961, 1447, 2016, 2713, 2746, 3159, 3275
EcoRV GATATC 2 cut(s) 298, 972
EcoT14I CCWWGG 4 cut(s) 256, 855, 2160, 3193
EcoT38I GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
ErhI CCWWGG 4 cut(s) 256, 855, 2160, 3193
Esp3I CGTCTC 1 cut(s) 323
FauI CCCGC 3 cut(s) 76, 232, 2038
FbaI TGATCA 4 cut(s) 1368, 2095, 2383, 3267
FblI GTMKAC 1 cut(s) 182
FokI GGATG 4 cut(s) 559, 1313, 3242, 3277
FriOI GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
FspBI CTAG 8 cut(s) 1143, 1551, 1992, 2232, 2847, 2883, 3194, 3219
GlaI GCGC 4 cut(s) 48, 1091, 1663, 1805
GsaI CCCAGC 3 cut(s) 1097, 2524, 2699
GsuI CTGGAG 2 cut(s) 984, 1119
HaeII RGCGCY 2 cut(s) 1093, 1665
HaeIII GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
HapII CCGG 7 cut(s) 50, 72, 270, 1258, 1306, 1652, 2964
HgaI GACGC 4 cut(s) 21, 62, 344, 966
HhaI GCGC 4 cut(s) 49, 1092, 1664, 1806
Hin6I GCGC 4 cut(s) 47, 1090, 1662, 1804
HinP1I GCGC 4 cut(s) 47, 1090, 1662, 1804
HincII GTYRAC 3 cut(s) 183, 1894, 2119
HindII GTYRAC 3 cut(s) 183, 1894, 2119
HinfI GANTC 9 cut(s) 179, 198, 276, 304, 644, 679, 1286, 1292, 1460
HpaI GTTAAC 1 cut(s) 1894
HpaII CCGG 7 cut(s) 50, 72, 270, 1258, 1306, 1652, 2964
Hpy99I CGWCG 5 cut(s) 37, 187, 284, 437, 1795
HpyCH4IV ACGT 3 cut(s) 848, 1793, 2631
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 53, 428, 2692, 2743, 3047, 3098
HpySE526I ACGT 3 cut(s) 848, 1793, 2631
HspAI GCGC 4 cut(s) 47, 1090, 1662, 1804
Ksp22I TGATCA 4 cut(s) 1368, 2095, 2383, 3267
KspAI GTTAAC 1 cut(s) 1894
Lsp1109I GCAGC 8 cut(s) 214, 502, 1975, 1990, 2033, 2698, 2887, 3163
LweI GCATC 7 cut(s) 431, 459, 581, 719, 1112, 1750, 2790
MaeI CTAG 8 cut(s) 1143, 1551, 1992, 2232, 2847, 2883, 3194, 3219
MaeII ACGT 3 cut(s) 848, 1793, 2631
MaeIII GTNAC 7 cut(s) 765, 1183, 1268, 1586, 2365, 2473, 2842
MbiI CCGCTC 2 cut(s) 89, 310
MfeI CAATTG 1 cut(s) 2705
MflI RGATCY 4 cut(s) 94, 126, 1969, 2108
MlyI GAGTC 8 cut(s) 188, 270, 1295, 1301, 2470, 2530, 2644, 2863
MmeI TCCRAC 3 cut(s) 129, 583, 1814
MroXI GAANNNNTTC 3 cut(s) 508, 1413, 1686
MslI CAYNNNNRTG 1 cut(s) 1782
MspA1I CMGCKG 5 cut(s) 227, 1097, 1433, 3114, 3154
MspCI CTTAAG 3 cut(s) 1823, 1847, 2311
MspI CCGG 7 cut(s) 50, 72, 270, 1258, 1306, 1652, 2964
MunI CAATTG 1 cut(s) 2705
Mva1269I GAATGC 1 cut(s) 3198
MvaI CCWGG 8 cut(s) 521, 963, 1449, 2018, 2715, 2748, 3161, 3277
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 7 cut(s) 38, 53, 428, 2692, 2743, 3047, 3098
NciI CCSGG 4 cut(s) 73, 1259, 1307, 2964
NlaIV GGNNCC 7 cut(s) 375, 430, 608, 922, 2427, 2758, 2916
NmuCI GTSAC 5 cut(s) 765, 1268, 1586, 2473, 2842
NspI RCATGY 4 cut(s) 1697, 1781, 2560, 2777
PagI TCATGA 1 cut(s) 2098
PaqCI CACCTGC 1 cut(s) 2480
PasI CCCWGGG 1 cut(s) 520
PciI ACATGT 2 cut(s) 2556, 2773
PctI GAATGC 1 cut(s) 3198
PdmI GAANNNNTTC 3 cut(s) 508, 1413, 1686
PfeI GAWTC 8 cut(s) 198, 304, 644, 679, 1460, 1682, 2083, 2446
PflMI CCANNNNNTGG 1 cut(s) 3166
PfoI TCCNGGA 1 cut(s) 2713
Ple19I CGATCG 1 cut(s) 157
PleI GAGTC 8 cut(s) 187, 270, 1294, 1300, 2470, 2530, 2644, 2863
PpsI GAGTC 8 cut(s) 187, 270, 1294, 1300, 2470, 2530, 2644, 2863
PpuMI RGGWCCY 1 cut(s) 607
PscI ACATGT 2 cut(s) 2556, 2773
PshBI ATTAAT 1 cut(s) 2135
PsiI TTATAA 2 cut(s) 1008, 1716
Psp124BI GAGCTC 2 cut(s) 191, 1075
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 8 cut(s) 519, 961, 1447, 2016, 2713, 2746, 3159, 3275
PspFI CCCAGC 3 cut(s) 1093, 2520, 2695
PspGI CCWGG 8 cut(s) 519, 961, 1447, 2016, 2713, 2746, 3159, 3275
PspN4I GGNNCC 7 cut(s) 375, 430, 608, 922, 2427, 2758, 2916
PspPI GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
PspPPI RGGWCCY 1 cut(s) 607
PstI CTGCAG 2 cut(s) 895, 919
PstNI CAGNNNCTG 5 cut(s) 890, 2342, 2747, 2789, 3166
PsuI RGATCY 4 cut(s) 94, 126, 1969, 2108
PvuI CGATCG 1 cut(s) 157
PvuII CAGCTG 2 cut(s) 1097, 3154
RsaI GTAC 8 cut(s) 62, 960, 1392, 2154, 2264, 2607, 2814, 3207
RsaNI GTAC 8 cut(s) 61, 959, 1391, 2153, 2263, 2606, 2813, 3206
RseI CAYNNNNRTG 1 cut(s) 1782
SacI GAGCTC 2 cut(s) 191, 1075
SalI GTCGAC 1 cut(s) 181
Sau96I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
SbfI CCTGCAGG 1 cut(s) 919
ScaI AGTACT 1 cut(s) 2154
SchI GAGTC 8 cut(s) 188, 270, 1295, 1301, 2470, 2530, 2644, 2863
SdaI CCTGCAGG 1 cut(s) 919
SfaNI GCATC 7 cut(s) 431, 459, 581, 719, 1112, 1750, 2790
SfcI CTRYAG 8 cut(s) 168, 891, 915, 1704, 1939, 2148, 2334, 2343
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
SmiMI CAYNNNNRTG 1 cut(s) 1782
SmlI CTYRAG 4 cut(s) 1823, 1847, 2311, 3257
SmoI CTYRAG 4 cut(s) 1823, 1847, 2311, 3257
SpeI ACTAGT 2 cut(s) 2846, 3218
Sse8387I CCTGCAGG 1 cut(s) 919
SspI AATATT 2 cut(s) 1012, 2053
SspMI CTAG 8 cut(s) 1143, 1551, 1992, 2232, 2847, 2883, 3194, 3219
SstI GAGCTC 2 cut(s) 191, 1075
StyI CCWWGG 4 cut(s) 256, 855, 2160, 3193
TaiI ACGT 3 cut(s) 851, 1796, 2634
TaqI TCGA 7 cut(s) 123, 138, 182, 282, 865, 2648, 2902
TatI WGTACW 1 cut(s) 2152
TauI GCSGC 3 cut(s) 71, 1604, 2460
TfiI GAWTC 8 cut(s) 198, 304, 644, 679, 1460, 1682, 2083, 2446
TscAI CASTG 7 cut(s) 633, 680, 1393, 1447, 3099, 3171, 3193
TseFI GTSAC 5 cut(s) 765, 1268, 1586, 2473, 2842
TseI GCWGC 8 cut(s) 227, 515, 1988, 2003, 2021, 2686, 2875, 3151
Tsp45I GTSAC 5 cut(s) 765, 1268, 1586, 2473, 2842
TspGWI ACGGA 5 cut(s) 48, 311, 2439, 2472, 2899
TspRI CASTG 7 cut(s) 633, 680, 1393, 1447, 3099, 3171, 3193
Van91I CCANNNNNTGG 1 cut(s) 3166
Vha464I CTTAAG 3 cut(s) 1823, 1847, 2311
VneI GTGCAC 3 cut(s) 1172, 1443, 3090
VpaK11BI GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
VspI ATTAAT 1 cut(s) 2135
XagI CCTNNNNNAGG 2 cut(s) 2126, 2751
XceI RCATGY 4 cut(s) 1697, 1781, 2560, 2777
XmaJI CCTAGG 1 cut(s) 3193
XmiI GTMKAC 1 cut(s) 182
XmnI GAANNNNTTC 3 cut(s) 508, 1413, 1686
XspI CTAG 8 cut(s) 1143, 1551, 1992, 2232, 2847, 2883, 3194, 3219
ZrmI AGTACT 1 cut(s) 2154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.