MD02G1300000.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010028
Physical Location & Seq
Reverse (-)
35472739 .. 35483295
10557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1300000.v1.1.491

Sequence Viewer

Length: 3342 bp
ATGGCGTGCGTAAAGGGGGTGAATCGATCGGCCTGCGTGGCGCTGGCTCCGGACGCGCCCTACATGGCAGCAGGGACGATGGCCGGAGCAGTGGATCTGTCCTTCAGCTCCTCCGCCAACATTGAGATCTTCAAGCTTGATTTACAGTCAGACGACCGTGATCTCCCGGTAGTGGGCGAGTCGACGAGCTCTGAGAGATTCAACAGGTTGTCATGGGCCAAGCCCACTGGATCGGGGTCGCAAGAATTCGGGCTTGGGCTCATCGCTGGTGGACTCGTTGATGGCACTATTGATATCTGGAACCCACAGACTCTGATCCGTCCGGAGGCGGGTGTAAGTGCTTCAGTTGGGCATCTCACAAGACATAAAGGCCCTGTTCGTGGTCTTGAATTTAATGCAATTGCACCCAATTTACTTGCATCTGGGGCTGATGATGGTGAAATTTGCATTTGGGACTTGACCAATCCTGCAGAACCCTCTCACTTTCCACCTCTCAAGGGTAGTGGATCTGCTGCCCAAGGTGAGGTGTCATTTTTATCTTGGAATAGCAAGGTCCAACATATATTAGCATCCACTTCATTAAATGGGACAACTGTGATTTGGGACCTGAAAAAGCAAAAGCCAGTAATAAGTTTTACAGATTCAGTTAGAAGACGGTGCTCTGTTTTGCAGTGGAATCCTGATATTGCCACTCAGCTAATTGTTTCTTCAGATGAAGATGGTTCACCTTCTCTTAGGCTGTGGGATATGCGTAATATAATGTCACCAGTTAAAGAGTTTGTCGGGCACACAAAAGGTGTAATTGCAATGTCTTGGTGTCCGAATGACAGCTCTTATCTGCTTACATGTGCCAAGGATAATCGAACTATTTGCTGGGACACAGTTTCTGCAGAGATTGTATGTGAATTGCCGGCAGGCACCAATTGGAATTTTGATGTGCATTGGTATCCAAAAGTACCTGGAGTGATATCGGCATCTTCATTTGATGGAAAAATTGGAATTTACAATATTGAGGGTTGCAGCAGATATGGAGTTGGGGAAAGTGATTTTGGTGCAGGACCTCTGAGAGCTCCGAAATGGTATAAGCGCCCAGTTGGGGCATCTTTTGGCTTTGGAGGCAAGATTGTGTCCTTTCAGCATACTTCTGCTGGTTCGGAGGTTTATGTACACAGCTTGGTTACTGAACACAGTTTGGTGAATCGCTCATCGGAATTTGAAGCTGCAATCCAAAATGGGGAAAGGCATTTACTTCGAGCCTTGTGTGAGAAGAAATCACAGGAGTCTGAATCTGAGGATGACCAGGAAACATGGGGTTTATTAAGGGTTATGTTTGAAGATGATGGGACTGCAAGGACGAAACTGATCACACATCTTGGTTTCAGTATGCCCGAGGAAACAAAAGAAGATGTTCCAGAGGATCTATCACAGGAAGTCGATGCACTTGGGCTTGATGATACTATAACTGATAAAGGGGGTCTTGGGAGTGATAAGGAAGCCACCATCTTTCCTTCTGATAACGGAGAAGATTTCTTCAATAATCTTCCTAGTCCTAAAGCTGATACTCCTGTGTCAACCTCTGGTGACAAATTATCTGTGGGGGATACAATTCCTGTTATGGAACAAATGGAACAAGAGTCTGATGGACTGGAGGAGAGTGCTGACCCATCATTTGATGAATCTGTTCAACATGCTTTAGTTGTAGGAGATTATAAGGGCGCTGTTGCAAAATGCATTTCAGCAAATAAAATAGCTGATGCTTTAGTTATTGCTCATGCTGGTGGTTCTTCATTGTGGGAGAGCACGCGGGATCAGTATCTTAAGATGAGCCATTCGCCTTACCTTAAGATTGTTTCTGCAATGGTGAGCAACGATCTCTTGAGTCTTGTCAACACCAGGCCTCTAAAGTTCTGGAAAGAAACACTTGCTCTTCTGTGTAGTTTTGCATCCAGAGACGAGTGGACTGTGCTTTGTGATACACTTGCTTCCAAACTTGTGGCTGCTGGTAATACGCTGGCGGCAACTATTTGTTATATATGTGCGGGAAATATTGATAAAACCGTAGAGATTTGGTCAAGAAGTCTGACCACTGAGCATGAGGGGAGATCCTATGTCGACCTCCTCCAGGAATTAATGGAGAAGACAATTGTCCTTGCCTTGGCATCTGGGCAGAAGCGGTTTAGTGCATCTTTGTGCAAACTTGTTGAGAAATACGCCGAAATTTTAGCTAGTCAAGGCCTTTTAACAACAGCAATGGAGTACCTAAAACTCTTGGGTTCTGATGAGTTGTCACCTGAGCTTGTTATCTTAAGAGATCGTATTTCTCTTTCAACAGAACCTGAAAAAGTTGCCAAGAATGAAACATTTGGAAACCAACCTGCAGCAAGTGGGCCTGTATATGCGGCTGATCAGTCTACTTTTGTGGGGGCCTCTCCTCCATATTATCAGGAAACAGTGCCATCACATTTGCAATCAGGTGTTCCTGTAAGTCCTTATGGCGAGAGCTACCAAGAACCTGTGAATCCTTCGTATGGAAGAGGAGGATATGGCCCTCCTGCCCCGTATCAGCCTGCATCCCAACCCCTGATGTTTCTTCCTAACCAACCACCTCAGGTTCCACAGAATTTTTCCGCACCTCCCGTTTCCTCTCAGCCCGCAGTAAGGCCCTTCATTCCTTCAACTCCTCCTGTGCTAAAAAATGTGGAGCAATATCAGCAGCCAACATTGGGTTCTCAGTTGTATCCTACTTTCCAACCTATGCAACCGGTGCCTGGTTCAACTGCTCCTCTTCAATCACAAGTCGCTCCAGTTCCTGTAAATAAACCACATGTTGTAGCTCCCTCTGTTCCTCCAAGAGGATTTATGCCAGTTACTAATCCAGGAGTTGTTCAGGGGCCTCATGCGGGTTCACTGGAGCCACCTAGTCCTACTCACCAAGCTCCAGCTCGTACTCCTGTGGCAGTAGCAGCTCCACCACCCACCATACAGACAGTTGATACATCCAGTGTTCCTGCCCATCAAAAATCGGTAATTACAACATTAACAAGACTTTTCAATGAGACATCGGAGGCGCTGGGAGGTGCACGTGCAAATCCCGGTAAGAAGCGTGAAATAGAAGACAATTCGAGGAAGATAGGTGCATTGTTTGCCAAACTCAACAGTGGGGACATATCTAGAAACGCTGCTGATAAGCTTGTTCAGCTTTGCCAGGCATTGGATAATGGTGATTTCGGTACTGCCCTACAAATCCAGGTGCTTCTTACCACTAGTGAATGGGACGAGTGCAACTTTTGGCTTGCAACTCTCAAGAGGATGATCAAGACAAGGCAAAATGTGAGACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SRA1 Sec16_C WD40

Protein Analysis

1114

Amino Acids

120.07

Weight (kDa)

5.12

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 87 - 251 1e-10 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 88 - 156 7.1e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 88 - 237 3.6e-09 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 88 - 239 1.6e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 88 - 251 2.2e-10 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 137 - 300 2.8e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 172 - 319 2.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 197 - 337 3.6e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 218 - 332 6.8e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 236 - 338 3.3e-10 WDR5 beta-propeller domain
TPR_GEMI5 PF23774 539 - 694 4.7e-07 GEMI5 TPR domain
TPR_Sec16 PF12931 561 - 756 3e-09 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63460 AT3G63460 AT3G63460
fragaria_vesca FvH4_7g01850 FvH4_7g01850 FvH4_7g01850
malus_domestica MD02G1300000.v1.1 MD07G1024900.v1.1
prunus_persica Prupe.2G024600_v2.0.a1
pyrus_communis pycom02g25200 pycom07g01920
rosa_chinensis RchiOBHm_Chr1g0318481
rosa_laevigata RLG00000030534
rosa_multiflora Rmu_ssc0000021.1_g000001
rosa_roxburghii Rroxscaffold_4G00329170
rosa_rugosa Rorug01G0021600.1
rosa_samantha Rh1AG034000 Rh1BG030000 Rh1CG033500 Rh1DG037100
rosa_wichuraiana Rw1G002700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1710
Acc36I ACCTGC 1 cut(s) 2383
AccB1I GGYRCC 2 cut(s) 917, 2764
AccB7I CCANNNNNTGG 1 cut(s) 3211
AccI GTMKAC 3 cut(s) 182, 2112, 2411
AccII CGCG 2 cut(s) 56, 1804
AccIII TCCGGA 2 cut(s) 49, 322
AclWI GGATC 7 cut(s) 102, 238, 310, 514, 1425, 1815, 2097
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 8 cut(s) 245, 389, 441, 928, 999, 1211, 2217, 2620
AcuI CTGAAG 3 cut(s) 88, 327, 693
AcvI CACGTG 1 cut(s) 3083
AfaI GTAC 5 cut(s) 957, 1167, 2258, 2947, 3232
AflII CTTAAG 3 cut(s) 1817, 1841, 2305
AflIII ACRYGT 2 cut(s) 845, 2824
AgeI ACCGGT 1 cut(s) 2761
AhlI ACTAGT 1 cut(s) 3263
AjnI CCWGG 8 cut(s) 958, 1299, 1892, 2121, 2767, 2875, 3204, 3246
AjuI GAANNNNNNNTTGG 6 cut(s) 237, 269, 1032, 1064, 2710, 2742
Alw21I GWGCWC 5 cut(s) 191, 662, 1072, 1802, 3082
Alw26I GTCTC 3 cut(s) 1944, 3050, 3328
Alw44I GTGCAC 1 cut(s) 3078
AlwI GGATC 7 cut(s) 102, 238, 310, 514, 1425, 1815, 2097
AlwNI CAGNNNCTG 4 cut(s) 313, 887, 2336, 2810
Ama87I CYCGRG 1 cut(s) 1388
Aor13HI TCCGGA 2 cut(s) 49, 322
ApaLI GTGCAC 1 cut(s) 3078
ApeKI GCWGC 9 cut(s) 68, 512, 1020, 1220, 1997, 2378, 2713, 2963, 3179
ApoI RAATTY 8 cut(s) 245, 389, 441, 928, 999, 1211, 2217, 2620
ArsI GACNNNNNNTTYG 2 cut(s) 1652, 1684
AseI ATTAAT 1 cut(s) 2129
AsiGI ACCGGT 1 cut(s) 2761
Asp700I GAANNNNTTC 2 cut(s) 1407, 1680
AspLEI GCGC 5 cut(s) 43, 58, 1089, 1718, 3070
AsuC2I CCSGG 2 cut(s) 167, 3093
AvaI CYCGRG 1 cut(s) 1388
AvaII GGWCC 3 cut(s) 553, 604, 1058
AxyI CCTNAGG 1 cut(s) 2607
BaeGI GKGCMC 2 cut(s) 789, 3082
BaeI ACNNNNGTAYC 2 cut(s) 1551, 1584
BanI GGYRCC 2 cut(s) 917, 2764
BanII GRGCYC 3 cut(s) 191, 261, 1072
BbrPI CACGTG 1 cut(s) 3083
BbsI GAAGAC 3 cut(s) 658, 2144, 3120
Bbv12I GWGCWC 5 cut(s) 191, 662, 1072, 1802, 3082
BbvI GCAGC 9 cut(s) 80, 499, 1032, 1207, 1984, 2390, 2725, 2975, 3166
BcgI CGANNNNNNTGC 6 cut(s) 15, 49, 852, 886, 1232, 1266
BciT130I CCWGG 8 cut(s) 960, 1301, 1894, 2123, 2769, 2877, 3206, 3248
BciVI GTATCC 3 cut(s) 957, 1594, 2748
BclI TGATCA 3 cut(s) 1362, 2404, 3312
BcnI CCSGG 2 cut(s) 167, 3093
BcoDI GTCTC 3 cut(s) 1944, 3050, 3328
BcuI ACTAGT 1 cut(s) 3263
BfaI CTAG 5 cut(s) 1545, 2226, 2919, 3171, 3264
BfmI CTRYAG 3 cut(s) 468, 888, 2376
BfoI RGCGCY 4 cut(s) 44, 1090, 1719, 3071
BfrI CTTAAG 3 cut(s) 1817, 1841, 2305
BfuAI ACCTGC 1 cut(s) 2383
BfuI GTATCC 3 cut(s) 957, 1594, 2748
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 1 cut(s) 126
Bme18I GGWCC 3 cut(s) 553, 604, 1058
BmeT110I CYCGRG 1 cut(s) 1388
BmiI GGNNCC 9 cut(s) 48, 302, 605, 919, 2425, 2613, 2766, 2892, 2913
BmrI ACTGGG 1 cut(s) 1085
BmuI ACTGGG 1 cut(s) 1085
BoxI GACNNNNGTC 1 cut(s) 2144
BpiI GAAGAC 3 cut(s) 658, 2144, 3120
BpmI CTGGAG 6 cut(s) 981, 1667, 2105, 2787, 2922, 2930
Bpu10I CCTNAGC 1 cut(s) 2292
BpuEI CTTGAG 3 cut(s) 479, 1897, 3287
BpuMI CCSGG 2 cut(s) 167, 3093
Bsa29I ATCGAT 1 cut(s) 25
BsaAI YACGTR 1 cut(s) 3083
BsaBI GATNNNNATC 1 cut(s) 1812
BsaJI CCNNGG 4 cut(s) 517, 852, 1389, 2154
BsaWI WCCGGW 3 cut(s) 49, 322, 2761
Bse118I RCCGGY 2 cut(s) 910, 2761
Bse1I ACTGG 9 cut(s) 232, 623, 767, 1091, 1650, 2804, 2864, 2913, 3000
Bse21I CCTNAGG 1 cut(s) 2607
Bse3DI GCAATG 3 cut(s) 813, 1863, 2256
Bse8I GATNNNNATC 1 cut(s) 1812
BseAI TCCGGA 2 cut(s) 49, 322
BseBI CCWGG 8 cut(s) 960, 1301, 1894, 2123, 2769, 2877, 3206, 3248
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 4 cut(s) 517, 852, 1389, 2154
BseGI GGATG 6 cut(s) 569, 1300, 1943, 2570, 2996, 3315
BseJI GATNNNNATC 1 cut(s) 1812
BseMI GCAATG 3 cut(s) 813, 1863, 2256
BseMII CTCAG 9 cut(s) 183, 707, 1055, 1281, 2079, 2283, 2621, 2660, 2744
BseNI ACTGG 9 cut(s) 232, 623, 767, 1091, 1650, 2804, 2864, 2913, 3000
BseRI GAGGAG 7 cut(s) 100, 1664, 2108, 2421, 2550, 2670, 2772
BseSI GKGCMC 2 cut(s) 789, 3082
BseXI GCAGC 9 cut(s) 80, 499, 1032, 1207, 1984, 2390, 2725, 2975, 3166
BseYI CCCAGC 2 cut(s) 873, 3070
BsgI GTGCAG 1 cut(s) 1074
Bsh1236I CGCG 2 cut(s) 56, 1804
Bsh1285I CGRYCG 2 cut(s) 29, 157
BshNI GGYRCC 2 cut(s) 917, 2764
BshTI ACCGGT 1 cut(s) 2761
BshVI ATCGAT 1 cut(s) 25
BsiEI CGRYCG 2 cut(s) 29, 157
BsiHKAI GWGCWC 5 cut(s) 191, 662, 1072, 1802, 3082
BsiHKCI CYCGRG 1 cut(s) 1388
BsiSI CCGG 7 cut(s) 50, 84, 167, 323, 911, 2762, 3093
BslFI GGGAC 8 cut(s) 88, 467, 601, 617, 890, 1357, 3176, 3287
BsmAI GTCTC 3 cut(s) 1944, 3050, 3328
BsmBI CGTCTC 1 cut(s) 1944
BsmFI GGGAC 8 cut(s) 88, 467, 601, 617, 890, 1357, 3176, 3287
BsoBI CYCGRG 1 cut(s) 1388
Bsp1286I GDGCHC 7 cut(s) 191, 261, 662, 789, 1072, 1802, 3082
Bsp13I TCCGGA 2 cut(s) 49, 322
Bsp1407I TGTACA 1 cut(s) 1165
BspCNI CTCAG 9 cut(s) 184, 706, 1056, 1282, 2080, 2284, 2620, 2659, 2743
BspDI ATCGAT 1 cut(s) 25
BspEI TCCGGA 2 cut(s) 49, 322
BspFNI CGCG 2 cut(s) 56, 1804
BspLI GGNNCC 9 cut(s) 48, 302, 605, 919, 2425, 2613, 2766, 2892, 2913
BspMAI CTGCAG 3 cut(s) 472, 892, 2380
BspMI ACCTGC 1 cut(s) 2383
BspPI GGATC 7 cut(s) 102, 238, 310, 514, 1425, 1815, 2097
BspQI GCTCTTC 1 cut(s) 1932
BspT107I GGYRCC 2 cut(s) 917, 2764
BspTI CTTAAG 3 cut(s) 1817, 1841, 2305
BsrDI GCAATG 3 cut(s) 813, 1863, 2256
BsrFI RCCGGY 2 cut(s) 910, 2761
BsrGI TGTACA 1 cut(s) 1165
BsrI ACTGG 9 cut(s) 232, 623, 767, 1091, 1650, 2804, 2864, 2913, 3000
BssAI RCCGGY 2 cut(s) 910, 2761
BssECI CCNNGG 4 cut(s) 517, 852, 1389, 2154
BssT1I CCWWGG 3 cut(s) 517, 852, 2154
Bst2UI CCWGG 8 cut(s) 960, 1301, 1894, 2123, 2769, 2877, 3206, 3248
Bst6I CTCTTC 3 cut(s) 1932, 2527, 2790
BstAFI CTTAAG 3 cut(s) 1817, 1841, 2305
BstAPI GCANNNNNTGC 2 cut(s) 2764, 3143
BstAUI TGTACA 1 cut(s) 1165
BstBAI YACGTR 1 cut(s) 3083
BstENI CCTNNNNNAGG 2 cut(s) 2120, 2850
BstF5I GGATG 6 cut(s) 569, 1300, 1943, 2570, 2996, 3315
BstFNI CGCG 2 cut(s) 56, 1804
BstH2I RGCGCY 4 cut(s) 44, 1090, 1719, 3071
BstHHI GCGC 5 cut(s) 43, 58, 1089, 1718, 3070
BstMAI GTCTC 3 cut(s) 1944, 3050, 3328
BstMCI CGRYCG 2 cut(s) 29, 157
BstMWI GCNNNNNNNGC 9 cut(s) 38, 53, 425, 1116, 2710, 2764, 2963, 3143, 3196
BstNI CCWGG 8 cut(s) 960, 1301, 1894, 2123, 2769, 2877, 3206, 3248
BstNSI RCATGY 3 cut(s) 849, 1691, 2828
BstPAI GACNNNNGTC 1 cut(s) 2144
BstSFI CTRYAG 3 cut(s) 468, 888, 2376
BstSLI GKGCMC 2 cut(s) 789, 3082
BstUI CGCG 2 cut(s) 56, 1804
BstV1I GCAGC 9 cut(s) 80, 499, 1032, 1207, 1984, 2390, 2725, 2975, 3166
BstV2I GAAGAC 3 cut(s) 658, 2144, 3120
BstX2I RGATCY 5 cut(s) 94, 126, 506, 1417, 2102
BstXI CCANNNNNNTGG 1 cut(s) 1993
BstYI RGATCY 5 cut(s) 94, 126, 506, 1417, 2102
Bsu15I ATCGAT 1 cut(s) 25
Bsu36I CCTNAGG 1 cut(s) 2607
BsuI GTATCC 3 cut(s) 957, 1594, 2748
BsuTUI ATCGAT 1 cut(s) 25
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 6 cut(s) 569, 1300, 1943, 2570, 2996, 3315
BtsI GCAGTG 2 cut(s) 96, 677
BtsIMutI CAGTG 8 cut(s) 96, 225, 677, 2085, 2457, 2906, 3007, 3163
BveI ACCTGC 1 cut(s) 2383
CaiI CAGNNNCTG 4 cut(s) 313, 887, 2336, 2810
CfoI GCGC 5 cut(s) 43, 58, 1089, 1718, 3070
Cfr10I RCCGGY 2 cut(s) 910, 2761
ClaI ATCGAT 1 cut(s) 25
CseI GACGC 1 cut(s) 62
Csp6I GTAC 5 cut(s) 956, 1166, 2257, 2946, 3231
CspAI ACCGGT 1 cut(s) 2761
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CviAII CATG 9 cut(s) 64, 213, 846, 1308, 1688, 1772, 2093, 2825, 2897
CviQI GTAC 5 cut(s) 956, 1166, 2257, 2946, 3231
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 3 cut(s) 1932, 2527, 2790
EarI CTCTTC 3 cut(s) 1932, 2527, 2790
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 2 cut(s) 189, 1070
Eco130I CCWWGG 3 cut(s) 517, 852, 2154
Eco147I AGGCCT 2 cut(s) 1897, 2235
Eco24I GRGCYC 3 cut(s) 191, 261, 1072
Eco32I GATATC 2 cut(s) 295, 969
Eco47I GGWCC 3 cut(s) 553, 604, 1058
Eco53kI GAGCTC 2 cut(s) 189, 1070
Eco57I CTGAAG 3 cut(s) 88, 327, 693
Eco72I CACGTG 1 cut(s) 3083
Eco81I CCTNAGG 1 cut(s) 2607
Eco88I CYCGRG 1 cut(s) 1388
EcoICRI GAGCTC 2 cut(s) 189, 1070
EcoNI CCTNNNNNAGG 2 cut(s) 2120, 2850
EcoO109I RGGNCCY 6 cut(s) 371, 604, 1058, 2424, 2661, 2891
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 8 cut(s) 958, 1299, 1892, 2121, 2767, 2875, 3204, 3246
EcoRV GATATC 2 cut(s) 295, 969
EcoT14I CCWWGG 3 cut(s) 517, 852, 2154
EcoT22I ATGCAT 1 cut(s) 1733
EcoT38I GRGCYC 3 cut(s) 191, 261, 1072
ErhI CCWWGG 3 cut(s) 517, 852, 2154
Esp3I CGTCTC 1 cut(s) 1944
FaeI CATG 9 cut(s) 67, 216, 849, 1311, 1691, 1775, 2096, 2828, 2900
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 1905, 1937
FaqI GGGAC 8 cut(s) 88, 467, 601, 617, 890, 1357, 3176, 3287
FatI CATG 9 cut(s) 63, 212, 845, 1307, 1687, 1771, 2092, 2824, 2896
FauI CCCGC 5 cut(s) 322, 1797, 2032, 2659, 2893
FbaI TGATCA 3 cut(s) 1362, 2404, 3312
FblI GTMKAC 3 cut(s) 182, 2112, 2411
FokI GGATG 6 cut(s) 556, 1307, 1930, 2557, 2983, 3322
FriOI GRGCYC 3 cut(s) 191, 261, 1072
FspBI CTAG 5 cut(s) 1545, 2226, 2919, 3171, 3264
GlaI GCGC 5 cut(s) 42, 57, 1088, 1717, 3069
GsaI CCCAGC 2 cut(s) 877, 3074
GsuI CTGGAG 6 cut(s) 981, 1667, 2105, 2787, 2922, 2930
HaeII RGCGCY 4 cut(s) 44, 1090, 1719, 3071
HapII CCGG 7 cut(s) 50, 84, 167, 323, 911, 2762, 3093
HgaI GACGC 1 cut(s) 62
HhaI GCGC 5 cut(s) 43, 58, 1089, 1718, 3070
Hin1II CATG 9 cut(s) 67, 216, 849, 1311, 1691, 1775, 2096, 2828, 2900
Hin6I GCGC 5 cut(s) 41, 56, 1087, 1716, 3068
HinP1I GCGC 5 cut(s) 41, 56, 1087, 1716, 3068
HincII GTYRAC 4 cut(s) 183, 1572, 1888, 2113
HindII GTYRAC 4 cut(s) 183, 1572, 1888, 2113
HindIII AAGCTT 2 cut(s) 134, 3188
HpaII CCGG 7 cut(s) 50, 84, 167, 323, 911, 2762, 3093
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 6 cut(s) 112, 738, 1518, 2532, 2674, 2682
HpyCH4IV ACGT 1 cut(s) 3082
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 53, 425, 1116, 2710, 2764, 2963, 3143, 3196
HpySE526I ACGT 1 cut(s) 3082
Hsp92II CATG 9 cut(s) 67, 216, 849, 1311, 1691, 1775, 2096, 2828, 2900
HspAI GCGC 5 cut(s) 41, 56, 1087, 1716, 3068
Kpn2I TCCGGA 2 cut(s) 49, 322
KroI GCCGGC 1 cut(s) 910
KroNI GCCGGC 1 cut(s) 912
Ksp22I TGATCA 3 cut(s) 1362, 2404, 3312
LguI GCTCTTC 1 cut(s) 1932
Lsp1109I GCAGC 9 cut(s) 80, 499, 1032, 1207, 1984, 2390, 2725, 2975, 3166
MaeI CTAG 5 cut(s) 1545, 2226, 2919, 3171, 3264
MaeII ACGT 1 cut(s) 3082
MaeIII GTNAC 5 cut(s) 762, 1177, 1580, 2286, 2866
MfeI CAATTG 3 cut(s) 399, 922, 2142
MflI RGATCY 5 cut(s) 94, 126, 506, 1417, 2102
MhlI GDGCHC 7 cut(s) 191, 261, 662, 789, 1072, 1802, 3082
MlyI GAGTC 6 cut(s) 188, 267, 304, 1289, 1643, 1888
MmeI TCCRAC 2 cut(s) 580, 2773
Mph1103I ATGCAT 1 cut(s) 1733
MroI TCCGGA 2 cut(s) 49, 322
MroNI GCCGGC 1 cut(s) 910
MroXI GAANNNNTTC 2 cut(s) 1407, 1680
MslI CAYNNNNRTG 2 cut(s) 1776, 2188
MspCI CTTAAG 3 cut(s) 1817, 1841, 2305
MspI CCGG 7 cut(s) 50, 84, 167, 323, 911, 2762, 3093
MunI CAATTG 3 cut(s) 399, 922, 2142
MvaI CCWGG 8 cut(s) 960, 1301, 1894, 2123, 2769, 2877, 3206, 3248
MvnI CGCG 2 cut(s) 56, 1804
MwoI GCNNNNNNNGC 9 cut(s) 38, 53, 425, 1116, 2710, 2764, 2963, 3143, 3196
NaeI GCCGGC 1 cut(s) 912
NciI CCSGG 2 cut(s) 167, 3093
NgoMIV GCCGGC 1 cut(s) 910
NlaIII CATG 9 cut(s) 67, 216, 849, 1311, 1691, 1775, 2096, 2828, 2900
NlaIV GGNNCC 9 cut(s) 48, 302, 605, 919, 2425, 2613, 2766, 2892, 2913
NmuCI GTSAC 3 cut(s) 762, 1580, 2286
NsiI ATGCAT 1 cut(s) 1733
NspI RCATGY 3 cut(s) 849, 1691, 2828
PceI AGGCCT 2 cut(s) 1897, 2235
PciI ACATGT 2 cut(s) 845, 2824
PciSI GCTCTTC 1 cut(s) 1932
PdiI GCCGGC 1 cut(s) 912
PdmI GAANNNNTTC 2 cut(s) 1407, 1680
PfeI GAWTC 8 cut(s) 22, 198, 641, 676, 1198, 1286, 1676, 2518
PflMI CCANNNNNTGG 1 cut(s) 3211
PfoI TCCNGGA 2 cut(s) 2121, 2875
PinAI ACCGGT 1 cut(s) 2761
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 6 cut(s) 187, 267, 304, 1288, 1642, 1887
PmaCI CACGTG 1 cut(s) 3083
PmlI CACGTG 1 cut(s) 3083
PpsI GAGTC 6 cut(s) 187, 267, 304, 1288, 1642, 1887
Ppu21I YACGTR 1 cut(s) 3083
PpuMI RGGWCCY 2 cut(s) 604, 1058
PscI ACATGT 2 cut(s) 845, 2824
PshAI GACNNNNGTC 1 cut(s) 2144
PshBI ATTAAT 1 cut(s) 2129
PsiI TTATAA 1 cut(s) 1710
Psp124BI GAGCTC 2 cut(s) 191, 1072
Psp5II RGGWCCY 2 cut(s) 604, 1058
Psp6I CCWGG 8 cut(s) 958, 1299, 1892, 2121, 2767, 2875, 3204, 3246
PspCI CACGTG 1 cut(s) 3083
PspFI CCCAGC 2 cut(s) 873, 3070
PspGI CCWGG 8 cut(s) 958, 1299, 1892, 2121, 2767, 2875, 3204, 3246
PspN4I GGNNCC 9 cut(s) 48, 302, 605, 919, 2425, 2613, 2766, 2892, 2913
PspPPI RGGWCCY 2 cut(s) 604, 1058
PstI CTGCAG 3 cut(s) 472, 892, 2380
PstNI CAGNNNCTG 4 cut(s) 313, 887, 2336, 2810
PsuI RGATCY 5 cut(s) 94, 126, 506, 1417, 2102
PvuI CGATCG 1 cut(s) 29
RsaI GTAC 5 cut(s) 957, 1167, 2258, 2947, 3232
RsaNI GTAC 5 cut(s) 956, 1166, 2257, 2946, 3231
RseI CAYNNNNRTG 2 cut(s) 1776, 2188
SacI GAGCTC 2 cut(s) 191, 1072
SalI GTCGAC 2 cut(s) 181, 2111
SapI GCTCTTC 1 cut(s) 1932
SchI GAGTC 6 cut(s) 188, 267, 304, 1289, 1643, 1888
SduI GDGCHC 7 cut(s) 191, 261, 662, 789, 1072, 1802, 3082
SfcI CTRYAG 3 cut(s) 468, 888, 2376
SinI GGWCC 3 cut(s) 553, 604, 1058
SmiMI CAYNNNNRTG 2 cut(s) 1776, 2188
SmlI CTYRAG 6 cut(s) 494, 1817, 1841, 1876, 2305, 3302
SmoI CTYRAG 6 cut(s) 494, 1817, 1841, 1876, 2305, 3302
SpeI ACTAGT 1 cut(s) 3263
SseBI AGGCCT 2 cut(s) 1897, 2235
SspI AATATT 2 cut(s) 1009, 2047
SspMI CTAG 5 cut(s) 1545, 2226, 2919, 3171, 3264
SstI GAGCTC 2 cut(s) 191, 1072
StuI AGGCCT 2 cut(s) 1897, 2235
StyI CCWWGG 3 cut(s) 517, 852, 2154
TaiI ACGT 1 cut(s) 3085
TaqI TCGA 7 cut(s) 25, 182, 862, 1252, 1434, 2112, 3122
TatI WGTACW 1 cut(s) 1165
TauI GCSGC 2 cut(s) 2018, 2402
TfiI GAWTC 8 cut(s) 22, 198, 641, 676, 1198, 1286, 1676, 2518
TscAI CASTG 8 cut(s) 96, 232, 677, 2092, 2457, 2913, 3007, 3163
TseFI GTSAC 3 cut(s) 762, 1580, 2286
TseI GCWGC 9 cut(s) 68, 512, 1020, 1220, 1997, 2378, 2713, 2963, 3179
Tsp45I GTSAC 3 cut(s) 762, 1580, 2286
TspDTI ATGAA 7 cut(s) 567, 729, 969, 1689, 1776, 2370, 2656
TspGWI ACGGA 2 cut(s) 308, 1533
TspRI CASTG 8 cut(s) 96, 232, 677, 2092, 2457, 2913, 3007, 3163
Van91I CCANNNNNTGG 1 cut(s) 3211
Vha464I CTTAAG 3 cut(s) 1817, 1841, 2305
VneI GTGCAC 1 cut(s) 3078
VpaK11BI GGWCC 3 cut(s) 553, 604, 1058
VspI ATTAAT 1 cut(s) 2129
XagI CCTNNNNNAGG 2 cut(s) 2120, 2850
XapI RAATTY 8 cut(s) 245, 389, 441, 928, 999, 1211, 2217, 2620
XbaI TCTAGA 1 cut(s) 3170
XceI RCATGY 3 cut(s) 849, 1691, 2828
XmiI GTMKAC 3 cut(s) 182, 2112, 2411
XmnI GAANNNNTTC 2 cut(s) 1407, 1680
XspI CTAG 5 cut(s) 1545, 2226, 2919, 3171, 3264
Zsp2I ATGCAT 1 cut(s) 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.