Rh1DG037100

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010028
Physical Location & Seq
Reverse (-)
5986281 .. 5997139
10859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG037100.1

Sequence Viewer

Length: 2742 bp
ATGGCGTGTATAAAGGGGGTGAACAGATCGGCGTCGGTGGCGGTCGCGCCGGACGCTCCGTACATGGCGGCCGGGACGATGGCGGGAGCAGTGGATCTGTCCTTCAGCTCCTCCGCGAATATCGAGATCTTCAAGCTCGATTTACAGTCGGACGATCGTGATCTTCCTGTAGTGGGAGAGTCCACGAGCTCCGAGAGATTCAACAGGTTATCGTGGTCCAAGCCCGCTGCTGGATCAGGCTCGCAAGACTTTGGCCTTGGGCTCATCGCCGGTGGACTCGTCGATGGGACTATTGATATCTGGAATCCGCTCACTCTGATCCGTTCTGAGACGGGGGAAAATGCTTCCGTTGAGCGTCTGACAAGACATAAGGGGCCTGTTCGTGGTCTTGAATTTAATGCAATCGCCCCAAATTTACTTGCATCTGGGGCCGACGATGGTGAAATTTGCATCTGGGACTTGGCTAATCCTACTGAGCCAACTCATTTTCCGCCTCTTAGGGGAAGTGGTTCTGCTGCCCAGGGTGAGATTTCATTTTTATCTTGGAATAGCAAGGTCCAACATATATTGGCATCCTCTTCGTATAATGGGACAACTGTGATTTGGGACCTAAAAAAGCAAAAGCCAGTGATAAGTTTTGCGGATTCAGTTAGAAGACGGTGCTCTGTTTTGCAGTGGAATCCTGATGTTGCCACTCAGCTTGTTGTTGCATCTGATGAAGATGGTTCACCTTCTCTCAGGCTCTGGGATATGCGTAATATATTGTCACCAGTTAAAGAGTTTGTGGGGCACACTAAAGGTGTAATTGCAATGTCATGGTGTCCTAATGACAGCTCTTATCTGCTAACGTGTGCCAAGGACAATCGAACTATTTGTTGGGACACAGTTTCTGCAGAGATTGTATGTGAATTGCCTGCAGGAACCAACTGGAATTTCGATGTGCATTGGTATCCGAAAGTACCTGGAGTGATATCAGCGTCTTCATTTGATGGAAAAATTGGAATTTATAATATTGAGGGTTGCAGTAGATATGGTGTGGGGGAGAGCGACTTTGGTGCTGGACCTCTCAGAGCTCCAAAATGGTATAAGCGCCCAGCTGGAGCATCTTTTGGTTTTGGAGGCAAGATTGTGTCCTTTCACCCTAGTTCATCTGGTGTTTCAGAGGTTTATGTGCACAGCTTGGTTACTGAACAAAGTTTGGTGGATCGCTCATCTGAATTTGAAAGTGCAATACAAAATGGGGAAAGGTCTTCACTCCGGGCTTTATGTGACAAGAAAGCACAGGAGTCTGAGTCTGCAGATGACCAGGAAACATGGGGCTTGTTAAGGGTTATGTTTGAAGATGATGGGACTGCAAGGACAAACCTGATCACACATCTTGGTTTCAGTGTACCTGAGGAAACAAAAGAAACAGTTCAAGATGGTCTATCAGCGGAAGTCAGTGCACCTGGGCTTGAGGATTCAACAACTGATAGTTTGGGTGCTGAGAAGGAAACTACAATTTTTCCTTCTGACAATGGAGAAGATTTCTTTAACAATCTTCCTAGTCCTAAAGCTGACACTCCTCATTCAACCTCTGGTGACAAATTTGTTGCGGCGGATACAGTTCCTATTACAGACCAAGTTCAAGAAGAACCTGATGAACCGGAAGAGAGCGCCGATCCTTCATTTGATGAATCAGTTCAACATGCTTTAGCTGTAGGAGATTATAAAGGGGCTGTTGCAAAGTGCATTTCAGCAAATAAAATGGCTGATGCTTTAGTTATTGCTCATGCTGGTGGTCCGACGTTGTGGGAGCGCACTCGGGATCAATACCTTAAGTTGAGCCATTCACCTTACCTTAAGATTGTTTCTGCAATGGTGAGCAATGATCTATCAAGTCTTGTTAACACCAGACCCCTCAAATTCTGGAAGGAAACACTTGCTGTTCTCTGTAGTTTTTCATCAATAGAGGCGTGGGAAGGTCTCTGTAATACGCTTGCTGCTAGACTTATAGCTGCTGGTAATACCCTGGCAGCAACTATTTGTTATATATGTGCGGGAAATATTGACAAGACGGTAGATATTTGGTCAAGGAATCTGACAACTGATCATGAAGGGAGATCCTATGTTGACCTTCTTCAGGAATTAATGGAGAAGACTATAGTACTTGCCTTGGCAACTGGACAGAAGCGATTTAGTGCTTCTTTGTGCAAACTTGTTGAGAAATATGCTGAAATTTTAGCTAGTCAAGGTCTTTTGACAACAGCAATGGAGTACCTGAAACTCTTGGGCTCTGATGAATTGTCTCCTGAACTAGTGATCTTAAGGGATCGTATTGCTCTTTCTACAGAACCCGTAGAGAAAGTCCCCAAGACTGTAACATTTGAAAACCCTGATCAAACCCCTTATGTCGGTGGGAACCCTCCATATTATCAGGAACCAGCTCAGCCACCTGTGAATCCGTATGGCGGCTATTCTGAACCTGTGACTCCTCCATATGCAGGTGGAGGATATGTCCCATACCCCCAGCCTCCATCACAGACTCCATCACAGCCTCCTAACATATTTGTTCCTACACCAACACCTCAACTACCACAGGAAAAATATCTTGTACCTCCTGCTTCTACTCAGCCTAACATGACCTTTATTCCTTCGACTCCTCCTGGTCTGCCAAATGTGGAGAAGTATCATCAGCCTACGCTGGGTTCTCAATTGTATCCTGTCGGTAGATCTGCACTTGATTTTAATTTACAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SRA1 Sec16_C WD40

Protein Analysis

913

Amino Acids

98.73

Weight (kDa)

4.78

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 88 - 240 2.1e-11 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 88 - 252 4.5e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 89 - 252 9.5e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 89 - 157 4.6e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 89 - 237 1.1e-09 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 89 - 238 6.8e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 138 - 301 7.9e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 173 - 320 5e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 198 - 338 5.9e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 219 - 333 6.7e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 245 - 339 5.5e-10 WDR5 beta-propeller domain
TPR_GEMI5 PF23774 555 - 693 8.8e-06 GEMI5 TPR domain
TPR_Sec16 PF12931 561 - 756 4.3e-09 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63460 AT3G63460 AT3G63460
fragaria_vesca FvH4_7g01850 FvH4_7g01850 FvH4_7g01850
malus_domestica MD02G1300000.v1.1 MD07G1024900.v1.1
prunus_persica Prupe.2G024600_v2.0.a1
pyrus_communis pycom02g25200 pycom07g01920
rosa_chinensis RchiOBHm_Chr1g0318481
rosa_laevigata RLG00000030534
rosa_multiflora Rmu_ssc0000021.1_g000001
rosa_roxburghii Rroxscaffold_4G00329170
rosa_rugosa Rorug01G0021600.1
rosa_samantha Rh1AG034000 Rh1BG030000 Rh1CG033500 Rh1DG037100
rosa_wichuraiana Rw1G002700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1008, 1710
AarI CACCTGC 1 cut(s) 2474
Acc36I ACCTGC 1 cut(s) 2474
AccBSI CCGCTC 1 cut(s) 310
AccII CGCG 2 cut(s) 47, 116
AclWI GGATC 8 cut(s) 102, 241, 313, 1212, 1655, 1815, 2097, 2319
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 9 cut(s) 392, 412, 444, 931, 1002, 1217, 1586, 1904, 2217
AcuI CTGAAG 2 cut(s) 88, 2105
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 6 cut(s) 62, 960, 1392, 2148, 2258, 2595
AfiI CCNNNNNNNGG 9 cut(s) 173, 230, 383, 500, 2122, 2393, 2450, 2483, 2684
AflII CTTAAG 3 cut(s) 1817, 1841, 2305
AflIII ACRYGT 1 cut(s) 848
AhlI ACTAGT 1 cut(s) 2296
AjnI CCWGG 6 cut(s) 519, 961, 1305, 1447, 2010, 2644
AjuI GAANNNNNNNTTGG 4 cut(s) 859, 891, 917, 949
Alw21I GWGCWC 5 cut(s) 191, 665, 1075, 1176, 1447
Alw26I GTCTC 3 cut(s) 323, 1970, 2292
Alw44I GTGCAC 2 cut(s) 1172, 1443
AlwI GGATC 8 cut(s) 102, 241, 313, 1212, 1655, 1815, 2097, 2319
AlwNI CAGNNNCTG 2 cut(s) 744, 890
Ama87I CYCGRG 1 cut(s) 1803
AoxI GGCC 4 cut(s) 69, 253, 374, 429
ApaLI GTGCAC 2 cut(s) 1172, 1443
ApeKI GCWGC 5 cut(s) 227, 515, 1982, 1997, 2015
ApoI RAATTY 9 cut(s) 392, 412, 444, 931, 1002, 1217, 1586, 1904, 2217
AseI ATTAAT 1 cut(s) 2129
Asp700I GAANNNNTTC 3 cut(s) 508, 1413, 1680
AspLEI GCGC 4 cut(s) 49, 1092, 1658, 1800
AspS9I GGNCC 7 cut(s) 216, 374, 429, 556, 607, 1061, 1781
AsuC2I CCSGG 2 cut(s) 73, 1259
AsuHPI GGTGA 9 cut(s) 31, 452, 536, 720, 759, 1130, 1592, 1824, 1873
AvaI CYCGRG 1 cut(s) 1803
AvaII GGWCC 5 cut(s) 216, 556, 607, 1061, 1781
AxyI CCTNAGG 1 cut(s) 1395
BaeGI GKGCMC 3 cut(s) 792, 1176, 1447
BanII GRGCYC 4 cut(s) 191, 264, 1075, 2276
BauI CACGAG 1 cut(s) 184
BbsI GAAGAC 4 cut(s) 661, 972, 1242, 2144
Bbv12I GWGCWC 5 cut(s) 191, 665, 1075, 1176, 1447
BbvI GCAGC 5 cut(s) 214, 502, 1969, 1984, 2027
BccI CCATC 9 cut(s) 73, 278, 431, 716, 983, 1340, 1415, 2524, 2536
BcgI CGANNNNNNTGC 4 cut(s) 561, 595, 1037, 1071
BciT130I CCWGG 6 cut(s) 521, 963, 1307, 1449, 2012, 2646
BciVI GTATCC 3 cut(s) 960, 1594, 2709
BclI TGATCA 3 cut(s) 1368, 2089, 2377
BcnI CCSGG 2 cut(s) 73, 1259
BcoDI GTCTC 3 cut(s) 323, 1970, 2292
BcuI ACTAGT 1 cut(s) 2296
BfaI CTAG 5 cut(s) 1143, 1545, 1986, 2226, 2297
BfmI CTRYAG 8 cut(s) 168, 891, 915, 1296, 1698, 1933, 2142, 2328
BfoI RGCGCY 2 cut(s) 1093, 1659
BfrI CTTAAG 3 cut(s) 1817, 1841, 2305
BfuAI ACCTGC 1 cut(s) 2474
BfuI GTATCC 3 cut(s) 960, 1594, 2709
BglII AGATCT 3 cut(s) 126, 2711, 2736
BisI GCNGC 8 cut(s) 69, 228, 516, 1596, 1983, 1998, 2016, 2452
BlpI GCTNAGC 1 cut(s) 2427
BlsI GCNGC 8 cut(s) 70, 229, 517, 1597, 1984, 1999, 2017, 2453
BmcAI AGTACT 1 cut(s) 2148
Bme1390I CCNGG 8 cut(s) 73, 521, 963, 1259, 1307, 1449, 2012, 2646
Bme18I GGWCC 5 cut(s) 216, 556, 607, 1061, 1781
BmeT110I CYCGRG 1 cut(s) 1803
BmgT120I GGNCC 7 cut(s) 216, 374, 429, 556, 607, 1061, 1781
BmiI GGNNCC 6 cut(s) 375, 430, 608, 922, 2402, 2421
BmrFI CCNGG 8 cut(s) 73, 521, 963, 1259, 1307, 1449, 2012, 2646
BmsI GCATC 6 cut(s) 431, 459, 581, 719, 1112, 1744
BpiI GAAGAC 4 cut(s) 661, 972, 1242, 2144
BpmI CTGGAG 2 cut(s) 984, 1119
Bpu1102I GCTNAGC 1 cut(s) 2427
BpuEI CTTGAG 1 cut(s) 1475
BpuMI CCSGG 2 cut(s) 73, 1259
BsaBI GATNNNNATC 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 1970
BsaJI CCNNGG 7 cut(s) 256, 519, 520, 855, 1448, 2010, 2154
BsaWI WCCGGW 1 cut(s) 1645
BsaXI ACNNNNNCTCC 4 cut(s) 1277, 1307, 2481, 2511
Bsc4I CCNNNNNNNGG 9 cut(s) 173, 230, 383, 500, 2122, 2393, 2450, 2483, 2684
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 4 cut(s) 626, 770, 932, 2167
Bse21I CCTNAGG 1 cut(s) 1395
Bse3DI GCAATG 4 cut(s) 816, 1863, 1873, 2256
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 6 cut(s) 521, 963, 1307, 1449, 2012, 2646
BseDI CCNNGG 7 cut(s) 256, 519, 520, 855, 1448, 2010, 2154
BseGI GGATG 1 cut(s) 572
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 9 cut(s) 173, 230, 383, 500, 2122, 2393, 2450, 2483, 2684
BseMI GCAATG 4 cut(s) 816, 1863, 1873, 2256
BseNI ACTGG 4 cut(s) 626, 770, 932, 2167
BseRI GAGGAG 4 cut(s) 100, 1554, 2463, 2631
BseSI GKGCMC 3 cut(s) 792, 1176, 1447
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 5 cut(s) 214, 502, 1969, 1984, 2027
BseYI CCCAGC 3 cut(s) 1093, 2508, 2683
BsgI GTGCAG 1 cut(s) 2700
Bsh1236I CGCG 2 cut(s) 47, 116
Bsh1285I CGRYCG 3 cut(s) 45, 72, 157
BshFI GGCC 4 cut(s) 71, 255, 376, 431
BsiEI CGRYCG 3 cut(s) 45, 72, 157
BsiHKAI GWGCWC 5 cut(s) 191, 665, 1075, 1176, 1447
BsiHKCI CYCGRG 1 cut(s) 1803
BsiSI CCGG 5 cut(s) 50, 72, 270, 1258, 1646
BslFI GGGAC 9 cut(s) 88, 301, 470, 604, 620, 893, 1363, 2333, 2483
BslI CCNNNNNNNGG 9 cut(s) 173, 230, 383, 500, 2122, 2393, 2450, 2483, 2684
BsmAI GTCTC 3 cut(s) 323, 1970, 2292
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 9 cut(s) 88, 301, 470, 604, 620, 893, 1363, 2333, 2483
BsnI GGCC 4 cut(s) 71, 255, 376, 431
Bso31I GGTCTC 1 cut(s) 1970
BsoBI CYCGRG 1 cut(s) 1803
Bsp1286I GDGCHC 8 cut(s) 191, 264, 665, 792, 1075, 1176, 1447, 2276
Bsp1720I GCTNAGC 1 cut(s) 2427
BspANI GGCC 4 cut(s) 71, 255, 376, 431
BspFNI CGCG 2 cut(s) 47, 116
BspHI TCATGA 1 cut(s) 2092
BspLI GGNNCC 6 cut(s) 375, 430, 608, 922, 2402, 2421
BspMAI CTGCAG 3 cut(s) 895, 919, 1300
BspMI ACCTGC 1 cut(s) 2474
BspPI GGATC 8 cut(s) 102, 241, 313, 1212, 1655, 1815, 2097, 2319
BspTI CTTAAG 3 cut(s) 1817, 1841, 2305
BspTNI GGTCTC 1 cut(s) 1970
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 4 cut(s) 816, 1863, 1873, 2256
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 4 cut(s) 626, 770, 932, 2167
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 7 cut(s) 256, 519, 520, 855, 1448, 2010, 2154
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 184
BssT1I CCWWGG 3 cut(s) 256, 855, 2154
Bst2BI CACGAG 1 cut(s) 184
Bst2UI CCWGG 6 cut(s) 521, 963, 1307, 1449, 2012, 2646
Bst4CI ACNGT 8 cut(s) 147, 598, 660, 886, 1414, 1606, 2059, 2359
Bst6I CTCTTC 2 cut(s) 583, 1644
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 3 cut(s) 1817, 1841, 2305
BstC8I GCNNGC 4 cut(s) 225, 242, 915, 1980
BstENI CCTNNNNNAGG 1 cut(s) 2120
BstF5I GGATG 1 cut(s) 572
BstFNI CGCG 2 cut(s) 47, 116
BstH2I RGCGCY 2 cut(s) 1093, 1659
BstHHI GCGC 4 cut(s) 49, 1092, 1658, 1800
BstMAI GTCTC 3 cut(s) 323, 1970, 2292
BstMCI CGRYCG 3 cut(s) 45, 72, 157
BstMWI GCNNNNNNNGC 3 cut(s) 38, 53, 428
BstNI CCWGG 6 cut(s) 521, 963, 1307, 1449, 2012, 2646
BstNSI RCATGY 1 cut(s) 1691
BstSCI CCNGG 8 cut(s) 71, 519, 961, 1257, 1305, 1447, 2010, 2644
BstSFI CTRYAG 8 cut(s) 168, 891, 915, 1296, 1698, 1933, 2142, 2328
BstSLI GKGCMC 3 cut(s) 792, 1176, 1447
BstUI CGCG 2 cut(s) 47, 116
BstV1I GCAGC 5 cut(s) 214, 502, 1969, 1984, 2027
BstV2I GAAGAC 4 cut(s) 661, 972, 1242, 2144
BstX2I RGATCY 5 cut(s) 94, 126, 2102, 2711, 2736
BstYI RGATCY 5 cut(s) 94, 126, 2102, 2711, 2736
BstZI CGGCCG 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 1395
BsuI GTATCC 3 cut(s) 960, 1594, 2709
BsuRI GGCC 4 cut(s) 71, 255, 376, 431
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 572
BtsI GCAGTG 2 cut(s) 96, 680
BtsIMutI CAGTG 5 cut(s) 96, 633, 680, 1393, 1447
BveI ACCTGC 1 cut(s) 2474
Cac8I GCNNGC 4 cut(s) 225, 242, 915, 1980
CaiI CAGNNNCTG 2 cut(s) 744, 890
CciI TCATGA 1 cut(s) 2092
CfoI GCGC 4 cut(s) 49, 1092, 1658, 1800
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 7 cut(s) 216, 374, 429, 556, 607, 1061, 1781
CseI GACGC 4 cut(s) 21, 62, 344, 966
Csp6I GTAC 6 cut(s) 61, 959, 1391, 2147, 2257, 2594
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviAII CATG 7 cut(s) 64, 816, 1314, 1688, 1772, 2093, 2620
CviQI GTAC 6 cut(s) 61, 959, 1391, 2147, 2257, 2594
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 583, 1644
EarI CTCTTC 2 cut(s) 583, 1644
EciI GGCGGA 2 cut(s) 480, 1613
Ecl136II GAGCTC 2 cut(s) 189, 1073
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 256, 855, 2154
Eco24I GRGCYC 4 cut(s) 191, 264, 1075, 2276
Eco31I GGTCTC 1 cut(s) 1970
Eco32I GATATC 2 cut(s) 298, 972
Eco47I GGWCC 5 cut(s) 216, 556, 607, 1061, 1781
Eco52I CGGCCG 1 cut(s) 69
Eco53kI GAGCTC 2 cut(s) 189, 1073
Eco57I CTGAAG 2 cut(s) 88, 2105
Eco81I CCTNAGG 1 cut(s) 1395
Eco88I CYCGRG 1 cut(s) 1803
EcoICRI GAGCTC 2 cut(s) 189, 1073
EcoNI CCTNNNNNAGG 1 cut(s) 2120
EcoO109I RGGNCCY 2 cut(s) 374, 607
EcoRII CCWGG 6 cut(s) 519, 961, 1305, 1447, 2010, 2644
EcoRV GATATC 2 cut(s) 298, 972
EcoT14I CCWWGG 3 cut(s) 256, 855, 2154
EcoT38I GRGCYC 4 cut(s) 191, 264, 1075, 2276
ErhI CCWWGG 3 cut(s) 256, 855, 2154
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 7 cut(s) 67, 819, 1317, 1691, 1775, 2096, 2623
FaqI GGGAC 9 cut(s) 88, 301, 470, 604, 620, 893, 1363, 2333, 2483
FatI CATG 7 cut(s) 63, 815, 1313, 1687, 1771, 2092, 2619
FauI CCCGC 3 cut(s) 76, 232, 2032
FauNDI CATATG 1 cut(s) 2479
FbaI TGATCA 3 cut(s) 1368, 2089, 2377
Fnu4HI GCNGC 8 cut(s) 69, 228, 516, 1596, 1983, 1998, 2016, 2452
FokI GGATG 1 cut(s) 559
FriOI GRGCYC 4 cut(s) 191, 264, 1075, 2276
Fsp4HI GCNGC 8 cut(s) 69, 228, 516, 1596, 1983, 1998, 2016, 2452
FspBI CTAG 5 cut(s) 1143, 1545, 1986, 2226, 2297
GlaI GCGC 4 cut(s) 48, 1091, 1657, 1799
GluI GCNGC 8 cut(s) 69, 228, 516, 1596, 1983, 1998, 2016, 2452
GsaI CCCAGC 3 cut(s) 1097, 2512, 2687
GsuI CTGGAG 2 cut(s) 984, 1119
HaeII RGCGCY 2 cut(s) 1093, 1659
HaeIII GGCC 4 cut(s) 71, 255, 376, 431
HapII CCGG 5 cut(s) 50, 72, 270, 1258, 1646
HgaI GACGC 4 cut(s) 21, 62, 344, 966
HhaI GCGC 4 cut(s) 49, 1092, 1658, 1800
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 7 cut(s) 67, 819, 1317, 1691, 1775, 2096, 2623
Hin6I GCGC 4 cut(s) 47, 1090, 1656, 1798
HinP1I GCGC 4 cut(s) 47, 1090, 1656, 1798
HincII GTYRAC 2 cut(s) 1888, 2113
HindII GTYRAC 2 cut(s) 1888, 2113
HpaI GTTAAC 1 cut(s) 1888
HpaII CCGG 5 cut(s) 50, 72, 270, 1258, 1646
HphI GGTGA 9 cut(s) 31, 452, 536, 720, 759, 1130, 1592, 1824, 1873
Hpy166II GTNNAC 9 cut(s) 22, 183, 275, 728, 1174, 1391, 1445, 1888, 2113
Hpy8I GTNNAC 9 cut(s) 22, 183, 275, 728, 1174, 1391, 1445, 1888, 2113
Hpy99I CGWCG 4 cut(s) 37, 284, 437, 1789
HpyCH4III ACNGT 8 cut(s) 147, 598, 660, 886, 1414, 1606, 2059, 2359
HpyCH4IV ACGT 2 cut(s) 848, 1787
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 53, 428
HpySE526I ACGT 2 cut(s) 848, 1787
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 7 cut(s) 67, 819, 1317, 1691, 1775, 2096, 2623
HspAI GCGC 4 cut(s) 47, 1090, 1656, 1798
Ksp22I TGATCA 3 cut(s) 1368, 2089, 2377
KspAI GTTAAC 1 cut(s) 1888
LmnI GCTCC 7 cut(s) 61, 86, 113, 194, 1078, 1100, 1795
Lsp1109I GCAGC 5 cut(s) 214, 502, 1969, 1984, 2027
LweI GCATC 6 cut(s) 431, 459, 581, 719, 1112, 1744
MaeI CTAG 5 cut(s) 1143, 1545, 1986, 2226, 2297
MaeII ACGT 2 cut(s) 848, 1787
MaeIII GTNAC 6 cut(s) 765, 1183, 1268, 1580, 2359, 2467
MbiI CCGCTC 1 cut(s) 310
MfeI CAATTG 1 cut(s) 2693
MflI RGATCY 5 cut(s) 94, 126, 2102, 2711, 2736
MhlI GDGCHC 8 cut(s) 191, 264, 665, 792, 1075, 1176, 1447, 2276
MlyI GAGTC 7 cut(s) 188, 270, 1295, 1301, 2464, 2518, 2632
MmeI TCCRAC 3 cut(s) 129, 583, 1808
MroXI GAANNNNTTC 3 cut(s) 508, 1413, 1680
MslI CAYNNNNRTG 1 cut(s) 1776
MspA1I CMGCKG 3 cut(s) 227, 1097, 1433
MspCI CTTAAG 3 cut(s) 1817, 1841, 2305
MspI CCGG 5 cut(s) 50, 72, 270, 1258, 1646
MspR9I CCNGG 8 cut(s) 73, 521, 963, 1259, 1307, 1449, 2012, 2646
MunI CAATTG 1 cut(s) 2693
MvaI CCWGG 6 cut(s) 521, 963, 1307, 1449, 2012, 2646
MvnI CGCG 2 cut(s) 47, 116
MwoI GCNNNNNNNGC 3 cut(s) 38, 53, 428
NciI CCSGG 2 cut(s) 73, 1259
NdeI CATATG 1 cut(s) 2479
NlaIII CATG 7 cut(s) 67, 819, 1317, 1691, 1775, 2096, 2623
NlaIV GGNNCC 6 cut(s) 375, 430, 608, 922, 2402, 2421
NmuCI GTSAC 4 cut(s) 765, 1268, 1580, 2467
NspI RCATGY 1 cut(s) 1691
PagI TCATGA 1 cut(s) 2092
PaqCI CACCTGC 1 cut(s) 2474
PasI CCCWGGG 1 cut(s) 520
PdmI GAANNNNTTC 3 cut(s) 508, 1413, 1680
PfeI GAWTC 8 cut(s) 198, 304, 644, 679, 1460, 1676, 2077, 2440
PkrI GCNGC 8 cut(s) 70, 229, 517, 1597, 1984, 1999, 2017, 2453
Ple19I CGATCG 1 cut(s) 157
PleI GAGTC 7 cut(s) 187, 270, 1294, 1300, 2464, 2518, 2632
PpsI GAGTC 7 cut(s) 187, 270, 1294, 1300, 2464, 2518, 2632
PpuMI RGGWCCY 1 cut(s) 607
PshBI ATTAAT 1 cut(s) 2129
PsiI TTATAA 2 cut(s) 1008, 1710
Psp124BI GAGCTC 2 cut(s) 191, 1075
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 6 cut(s) 519, 961, 1305, 1447, 2010, 2644
PspFI CCCAGC 3 cut(s) 1093, 2508, 2683
PspGI CCWGG 6 cut(s) 519, 961, 1305, 1447, 2010, 2644
PspN4I GGNNCC 6 cut(s) 375, 430, 608, 922, 2402, 2421
PspPI GGNCC 7 cut(s) 216, 374, 429, 556, 607, 1061, 1781
PspPPI RGGWCCY 1 cut(s) 607
PstI CTGCAG 3 cut(s) 895, 919, 1300
PstNI CAGNNNCTG 2 cut(s) 744, 890
PsuI RGATCY 5 cut(s) 94, 126, 2102, 2711, 2736
PvuI CGATCG 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 6 cut(s) 62, 960, 1392, 2148, 2258, 2595
RsaNI GTAC 6 cut(s) 61, 959, 1391, 2147, 2257, 2594
RseI CAYNNNNRTG 1 cut(s) 1776
SacI GAGCTC 2 cut(s) 191, 1075
SatI GCNGC 8 cut(s) 69, 228, 516, 1596, 1983, 1998, 2016, 2452
Sau96I GGNCC 7 cut(s) 216, 374, 429, 556, 607, 1061, 1781
SbfI CCTGCAGG 1 cut(s) 919
ScaI AGTACT 1 cut(s) 2148
SchI GAGTC 7 cut(s) 188, 270, 1295, 1301, 2464, 2518, 2632
ScrFI CCNGG 8 cut(s) 73, 521, 963, 1259, 1307, 1449, 2012, 2646
SdaI CCTGCAGG 1 cut(s) 919
SduI GDGCHC 8 cut(s) 191, 264, 665, 792, 1075, 1176, 1447, 2276
SfaNI GCATC 6 cut(s) 431, 459, 581, 719, 1112, 1744
SfcI CTRYAG 8 cut(s) 168, 891, 915, 1296, 1698, 1933, 2142, 2328
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 5 cut(s) 216, 556, 607, 1061, 1781
SmiMI CAYNNNNRTG 1 cut(s) 1776
SmlI CTYRAG 4 cut(s) 1454, 1817, 1841, 2305
SmoI CTYRAG 4 cut(s) 1454, 1817, 1841, 2305
SpeI ACTAGT 1 cut(s) 2296
Sse8387I CCTGCAGG 1 cut(s) 919
SspI AATATT 2 cut(s) 1012, 2047
SspMI CTAG 5 cut(s) 1143, 1545, 1986, 2226, 2297
SstI GAGCTC 2 cut(s) 191, 1075
StyD4I CCNGG 8 cut(s) 71, 519, 961, 1257, 1305, 1447, 2010, 2644
StyI CCWWGG 3 cut(s) 256, 855, 2154
TaaI ACNGT 8 cut(s) 147, 598, 660, 886, 1414, 1606, 2059, 2359
TaiI ACGT 2 cut(s) 851, 1790
TaqI TCGA 6 cut(s) 123, 138, 282, 865, 936, 2636
TatI WGTACW 1 cut(s) 2146
TauI GCSGC 3 cut(s) 71, 1598, 2454
TfiI GAWTC 8 cut(s) 198, 304, 644, 679, 1460, 1676, 2077, 2440
TscAI CASTG 5 cut(s) 96, 633, 680, 1393, 1447
TseFI GTSAC 4 cut(s) 765, 1268, 1580, 2467
TseI GCWGC 5 cut(s) 227, 515, 1982, 1997, 2015
Tsp45I GTSAC 4 cut(s) 765, 1268, 1580, 2467
TspGWI ACGGA 4 cut(s) 48, 311, 337, 2433
TspRI CASTG 5 cut(s) 96, 633, 680, 1393, 1447
Vha464I CTTAAG 3 cut(s) 1817, 1841, 2305
VneI GTGCAC 2 cut(s) 1172, 1443
VpaK11BI GGWCC 5 cut(s) 216, 556, 607, 1061, 1781
VspI ATTAAT 1 cut(s) 2129
XagI CCTNNNNNAGG 1 cut(s) 2120
XapI RAATTY 9 cut(s) 392, 412, 444, 931, 1002, 1217, 1586, 1904, 2217
XceI RCATGY 1 cut(s) 1691
XmnI GAANNNNTTC 3 cut(s) 508, 1413, 1680
XspI CTAG 5 cut(s) 1143, 1545, 1986, 2226, 2297
ZrmI AGTACT 1 cut(s) 2148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.