AT3G66656
MADS Family

Agamous-like MADS-box protein AGL61

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012972
Physical Location & Seq
Reverse (-)
2091262 .. 2091798
537 bp
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UTR
Exon/CDS
Intron
AT3G66656.1

Sequence Viewer

Length: 537 bp
ATGGGTAGGAGAAAGATTAAGATGGAGAAAGTGCAAGACACAAACACGAAGCAAGTTACCTTCTCAAAACGTAGGCTGGGTTTGTTCAAGAAAGCGAGCGAGCTTGCGACTCTGTGCAACGCGGAGGTTGGTATTGTTGTCTTTTCTCCAGGAAACAAACCGTATTCCTTCGGGAAACCGAATTTTGATGTGATTGCAGAACGGTTTAAGAATGAATTCGAAGAAGAAGAAGAAGGAGATAGCTGTGAAACATCAGGCTATAGTAGAGGCAATAGAGCTAGACAGGAGAAGAAGATATGTAAACGCCTCAACTCGATTACTGAAGAAGCTGAAGCTGAGAAGAAACATGGTGAAGATCTTCACAAGTGGCTTGAATCTGCTGAACAGGATAAGTTCAATAAGCCCATTGAGGAGCTTACGCTTGAGGAACTCAAGGAATTTGAGGCTAAGATTAAGAAAATAAGCTGTGGGATCCAAAGTAACATTAGTCATATGCAGGCTTCGTCTTCTCTCATGTTTCTCTCTAATGATAATTAG

Protein Analysis

178

Amino Acids

20.37

Weight (kDa)

6.85

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 8.6e-24 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34440 AT3G66656
fragaria_vesca FvH4_3g05940 FvH4_3g36320
malus_domestica MD05G1287100.v1.1 MD10G1264500.v1.1
prunus_persica Prupe.4G077800_v2.0.a1
pyrus_communis pycom05g26560
rosa_chinensis RchiOBHm_Chr5g0013531
rosa_laevigata RLG00000032023
rosa_multiflora Rmu_sc0014653.1_g000002
rosa_roxburghii Rroxscaffold_1G00063040
rosa_rugosa Rorug05G0010300
rosa_samantha Rh5AG105300 Rh5BG101400 Rh5CG113400 Rh5DG100100
rosa_wichuraiana Rw5G009160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 122
AciI CCGC 1 cut(s) 122
AclWI GGATC 2 cut(s) 466, 479
AcsI RAATTY 3 cut(s) 181, 215, 437
AcuI CTGAAG 2 cut(s) 342, 351
AgsI TTSAA 3 cut(s) 88, 374, 397
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 7 cut(s) 103, 243, 278, 329, 335, 415, 465
AluI AGCT 7 cut(s) 103, 243, 278, 329, 335, 415, 465
AlwI GGATC 2 cut(s) 466, 479
ApoI RAATTY 3 cut(s) 181, 215, 437
Asp700I GAANNNNTTC 2 cut(s) 215, 357
AsuHPI GGTGA 1 cut(s) 362
AsuII TTCGAA 1 cut(s) 219
BamHI GGATCC 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 498
BccI CCATC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 150
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 259
BglII AGATCT 1 cut(s) 355
Bme1390I CCNGG 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 473
BmrFI CCNGG 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 498
BpmI CTGGAG 1 cut(s) 132
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 2 cut(s) 416, 443
BseBI CCWGG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 327
BseRI GAGGAG 1 cut(s) 425
BseYI CCCAGC 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 122
Bsp119I TTCGAA 1 cut(s) 219
Bsp143I GATC 2 cut(s) 355, 471
BspACI CCGC 1 cut(s) 122
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 473
BspPI GGATC 2 cut(s) 466, 479
BspT104I TTCGAA 1 cut(s) 219
BssMI GATC 2 cut(s) 355, 471
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 162, 204
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 4 cut(s) 97, 101, 105, 498
BstDEI CTNAG 2 cut(s) 336, 447
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 2 cut(s) 358, 474
BstMBI GATC 2 cut(s) 355, 471
BstNI CCWGG 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 1 cut(s) 259
BstUI CGCG 1 cut(s) 122
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 2 cut(s) 355, 471
BstYI RGATCY 2 cut(s) 355, 471
Cac8I GCNNGC 4 cut(s) 97, 101, 105, 498
CviAII CATG 2 cut(s) 347, 514
DdeI CTNAG 2 cut(s) 336, 447
DpnI GATC 2 cut(s) 357, 473
DpnII GATC 2 cut(s) 355, 471
Eco57I CTGAAG 2 cut(s) 342, 351
EcoRI GAATTC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 148
FaeI CATG 2 cut(s) 350, 517
FaiI YATR 6 cut(s) 261, 298, 348, 492, 494, 515
FatI CATG 2 cut(s) 346, 513
FauNDI CATATG 1 cut(s) 492
FspBI CTAG 1 cut(s) 279
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 132
Hin1II CATG 2 cut(s) 350, 517
HinfI GANTC 2 cut(s) 109, 374
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 1 cut(s) 302
Hpy188III TCNNGA 2 cut(s) 88, 172
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 3 cut(s) 70, 178, 227
HpyCH4III ACNGT 2 cut(s) 162, 204
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 4 cut(s) 34, 117, 197, 496
HpyF3I CTNAG 2 cut(s) 336, 447
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 2 cut(s) 350, 517
Kzo9I GATC 2 cut(s) 355, 471
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 7 cut(s) 62, 135, 162, 240, 269, 371, 482
MaeI CTAG 1 cut(s) 279
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 2 cut(s) 55, 479
MalI GATC 2 cut(s) 357, 473
MboI GATC 2 cut(s) 355, 471
MflI RGATCY 2 cut(s) 355, 471
MluCI AATT 4 cut(s) 181, 215, 437, 532
MlyI GAGTC 1 cut(s) 103
MnlI CCTC 6 cut(s) 118, 260, 317, 403, 418, 436
MroXI GAANNNNTTC 2 cut(s) 215, 357
MseI TTAA 3 cut(s) 18, 207, 453
MspR9I CCNGG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 122
NdeI CATATG 1 cut(s) 492
NdeII GATC 2 cut(s) 355, 471
NlaIII CATG 2 cut(s) 350, 517
NlaIV GGNNCC 1 cut(s) 473
NspV TTCGAA 1 cut(s) 219
PdmI GAANNNNTTC 2 cut(s) 215, 357
PfeI GAWTC 1 cut(s) 374
PfoI TCCNGGA 1 cut(s) 148
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 148
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 473
PsuI RGATCY 2 cut(s) 355, 471
SaqAI TTAA 3 cut(s) 18, 207, 453
Sau3AI GATC 2 cut(s) 355, 471
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 259
SfuI TTCGAA 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 422, 431
SmoI CTYRAG 2 cut(s) 422, 431
Sse9I AATT 4 cut(s) 181, 215, 437, 532
SsiI CCGC 1 cut(s) 122
SspMI CTAG 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 162, 204
TaiI ACGT 1 cut(s) 73
TaqI TCGA 2 cut(s) 219, 314
TasI AATT 4 cut(s) 181, 215, 437, 532
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 3 cut(s) 18, 207, 453
Tru9I TTAA 3 cut(s) 18, 207, 453
TspDTI ATGAA 1 cut(s) 228
XapI RAATTY 3 cut(s) 181, 215, 437
XmnI GAANNNNTTC 2 cut(s) 215, 357
XspI CTAG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.