AT4G00950

Protein of unknown function (DUF688)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014982
Physical Location & Seq
Reverse (-)
405623 .. 407142
1520 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00950.1

Sequence Viewer

Length: 876 bp
ATGGAAGCTGAGAAAGAAACAGAACAAGAAGGAAACTTGACAGTAATGAAACTTCCGGTGTTACCAACTAAACCCAACACTCACAGCCACTCTATGTCATCACCAATTCACAGTTCCATATCAGCTTCAGTTCCCTTTAGCTGGGAAGAAGAGCCTGGCAAGCCCAAGCAACACTCTACTTCTTCTTCCTCCTCTTCCTCTTCCTCACCATTAACTTCTTATTCTTCATCTCCTTTTGAAACTCACAAGTCCTTAGAGCTACCACCAAGGCTTCACTTACTTGAAAAAGATGGAGGATCAGTAACCAAACTTCACTCGCCTATAACAGTCTTTGATGGACCTTATAGCATGACGACATCAAAAAGGATGGATTCACCTTCCTTTAGGATGATGGTGAAAGGTAGTGCTGATTGTTATGGGTCTTTCAGGAGTGATATCGATGGTGATTTAGAGGATTTGGAAGTGGGCTCTAAGCAGCAGGAGAACTTGAGTAGTGGCTCTTTGGCTGTTGTGAAGAAGAGAGGGAGATTAGGGTTTTTTGGGTTTAGGAGGAGGAGAGCATTGAAAGGGAAAACAGAGTTTGGTAGAGGTAGTTATGTGTTCCCATCTTCTGTAGACAGAGAGAGTGAATACAGTAGAAAGGAAGAGGAAGAAGAAAAAGAAGACAAAAGGTTTGGTTATGATGATACTGATGGTATTAGTTGCAGCCAAAGCAGCAGGTTTTGTGATGTGAAGATCTCAAGCATTAGTAGAACAGGCAGCTTCTCTACTCTGCCTGCACCACCTTCTTCTTCTTCAAAGTCTCACTTTTGGACGAATGTGTATGCAGGACTAAAGCAAGTGGTTCCATGGAAGAGCAAGAAGACTACAGTTTGA

Protein Analysis

291

Amino Acids

32.17

Weight (kDa)

8.6

Isoelectric Point (pI)

69.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 11 - 250 1.7e-08 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00950
fragaria_vesca FvH4_7g16850
malus_domestica MD01G1083500.v1.1 MD07G1152200.v1.1
prunus_persica Prupe.2G190500_v2.0.a1
pyrus_communis pycom07g15030
rosa_chinensis RchiOBHm_Chr1g0360071
rosa_laevigata RLG00000027835
rosa_multiflora Rmu_ssc0000368.1_g000038
rosa_roxburghii Rroxscaffold_4G00296400
rosa_rugosa Rorug01G0274700
rosa_samantha Rh1AG288700 Rh1BG254200 Rh1CG271700 Rh1DG283300
rosa_wichuraiana Rw1G025600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 708
AccI GTMKAC 1 cut(s) 615
AclWI GGATC 1 cut(s) 304
AcuI CTGAAG 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 4 cut(s) 239, 284, 565, 798
AjnI CCWGG 1 cut(s) 154
AjuI GAANNNNNNNTTGG 2 cut(s) 97, 129
AluBI AGCT 5 cut(s) 8, 125, 141, 259, 762
AluI AGCT 5 cut(s) 8, 125, 141, 259, 762
Alw26I GTCTC 1 cut(s) 807
AlwI GGATC 1 cut(s) 304
ApeKI GCWGC 4 cut(s) 475, 705, 714, 759
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 5 cut(s) 93, 198, 366, 406, 455
AvaII GGWCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 470
BarI GAAGNNNNNNTAC 4 cut(s) 36, 68, 294, 326
BbsI GAAGAC 2 cut(s) 669, 869
BbvI GCAGC 4 cut(s) 487, 717, 726, 771
BccI CCATC 7 cut(s) 284, 329, 361, 385, 434, 613, 686
BciT130I CCWGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 807
BfmI CTRYAG 2 cut(s) 612, 867
BfuAI ACCTGC 1 cut(s) 708
BglII AGATCT 1 cut(s) 735
BisI GCNGC 4 cut(s) 476, 706, 715, 760
BlsI GCNGC 4 cut(s) 477, 707, 716, 761
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 846
BmrFI CCNGG 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 669, 869
BpuEI CTTGAG 2 cut(s) 508, 724
Bsa29I ATCGAT 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 266, 848
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 141
BseBI CCWGG 1 cut(s) 156
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 266, 848
BseGI GGATG 2 cut(s) 372, 393
BseLI CCNNNNNNNGG 1 cut(s) 141
BseRI GAGGAG 3 cut(s) 181, 565, 568
BseXI GCAGC 4 cut(s) 487, 717, 726, 771
BseYI CCCAGC 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 762
BshVI ATCGAT 1 cut(s) 438
BsiSI CCGG 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 807
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 2 cut(s) 296, 735
Bsp19I CCATGG 1 cut(s) 848
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 846
BspMI ACCTGC 1 cut(s) 708
BspPI GGATC 1 cut(s) 304
BspQI GCTCTTC 2 cut(s) 144, 848
BssECI CCNNGG 2 cut(s) 266, 848
BssMI GATC 2 cut(s) 296, 735
BssT1I CCWWGG 2 cut(s) 266, 848
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 5 cut(s) 43, 113, 328, 635, 871
Bst6I CTCTTC 6 cut(s) 144, 199, 205, 512, 639, 848
BstC8I GCNNGC 2 cut(s) 161, 777
BstDEI CTNAG 3 cut(s) 9, 253, 471
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 2 cut(s) 372, 393
BstKTI GATC 2 cut(s) 299, 738
BstMAI GTCTC 1 cut(s) 807
BstMBI GATC 2 cut(s) 296, 735
BstMWI GCNNNNNNNGC 3 cut(s) 160, 711, 714
BstNI CCWGG 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 2 cut(s) 612, 867
BstV1I GCAGC 4 cut(s) 487, 717, 726, 771
BstV2I GAAGAC 2 cut(s) 669, 869
BstX2I RGATCY 1 cut(s) 735
BstYI RGATCY 1 cut(s) 735
Bsu15I ATCGAT 1 cut(s) 438
BsuTUI ATCGAT 1 cut(s) 438
BtgI CCRYGG 1 cut(s) 848
BtsCI GGATG 2 cut(s) 372, 393
BveI ACCTGC 1 cut(s) 708
Cac8I GCNNGC 2 cut(s) 161, 777
Cfr13I GGNCC 1 cut(s) 338
ClaI ATCGAT 1 cut(s) 438
CviAII CATG 2 cut(s) 349, 849
DdeI CTNAG 3 cut(s) 9, 253, 471
DpnI GATC 2 cut(s) 298, 737
DpnII GATC 2 cut(s) 296, 735
Eam1104I CTCTTC 6 cut(s) 144, 199, 205, 512, 639, 848
EarI CTCTTC 6 cut(s) 144, 199, 205, 512, 639, 848
Eco130I CCWWGG 2 cut(s) 266, 848
Eco24I GRGCYC 1 cut(s) 470
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 154
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 2 cut(s) 266, 848
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 2 cut(s) 266, 848
FaeI CATG 2 cut(s) 352, 852
FalI AAGNNNNNCTT 2 cut(s) 791, 823
FatI CATG 2 cut(s) 348, 848
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 4 cut(s) 476, 706, 715, 760
FokI GGATG 2 cut(s) 379, 400
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 4 cut(s) 476, 706, 715, 760
GluI GCNGC 4 cut(s) 476, 706, 715, 760
GsaI CCCAGC 1 cut(s) 145
HapII CCGG 1 cut(s) 56
Hin1II CATG 2 cut(s) 352, 852
HinfI GANTC 1 cut(s) 371
HpaII CCGG 1 cut(s) 56
HphI GGTGA 5 cut(s) 93, 198, 366, 406, 455
Hpy166II GTNNAC 1 cut(s) 616
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 1 cut(s) 616
HpyAV CCTTC 3 cut(s) 23, 387, 795
HpyCH4III ACNGT 5 cut(s) 43, 113, 328, 635, 871
HpyCH4V TGCA 3 cut(s) 705, 779, 827
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 711, 714
HpyF3I CTNAG 3 cut(s) 9, 253, 471
Hsp92II CATG 2 cut(s) 352, 852
Kzo9I GATC 2 cut(s) 296, 735
LguI GCTCTTC 2 cut(s) 144, 848
Lsp1109I GCAGC 4 cut(s) 487, 717, 726, 771
MaeIII GTNAC 2 cut(s) 60, 301
MalI GATC 2 cut(s) 298, 737
MboI GATC 2 cut(s) 296, 735
MflI RGATCY 1 cut(s) 735
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 1 cut(s) 105
MseI TTAA 1 cut(s) 212
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 156
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 160, 711, 714
NcoI CCATGG 1 cut(s) 848
NdeII GATC 2 cut(s) 296, 735
NlaIII CATG 2 cut(s) 352, 852
NlaIV GGNNCC 1 cut(s) 846
PciSI GCTCTTC 2 cut(s) 144, 848
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 4 cut(s) 477, 707, 716, 761
Psp6I CCWGG 1 cut(s) 154
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 846
PspPI GGNCC 1 cut(s) 338
PsuI RGATCY 1 cut(s) 735
SapI GCTCTTC 2 cut(s) 144, 848
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 4 cut(s) 476, 706, 715, 760
Sau3AI GATC 2 cut(s) 296, 735
Sau96I GGNCC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 1 cut(s) 470
SfcI CTRYAG 2 cut(s) 612, 867
SinI GGWCC 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 487, 739
SmoI CTYRAG 2 cut(s) 487, 739
Sse9I AATT 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 2 cut(s) 266, 848
TaaI ACNGT 5 cut(s) 43, 113, 328, 635, 871
TaqI TCGA 1 cut(s) 438
TasI AATT 1 cut(s) 105
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TseI GCWGC 4 cut(s) 475, 705, 714, 759
TspDTI ATGAA 2 cut(s) 62, 216
VpaK11BI GGWCC 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.