pycom07g15030

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0014982
Physical Location & Seq
Forward (+)
17107663 .. 17109293
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g15030.1

Sequence Viewer

Length: 690 bp
ATGGGGCTGGGATCTGATGAGGCAGAGCCGCCACAACTGCCCTTATTTTCTGCCCCACCATTTCACTCCCATGAGCCTTCAGGCACCCTAACTCCACCACTCCACACCTCAGTCTCAGTCCCATTCCGCTGGGAAGAGCAGCCCGGGAAGCCAAGGCCCTGCACTGTCCTGGCCACCATTCCAAACCCAACTGACTTTTCCCGAAAGTGCTTAGAGCTCCCTCCAAGGCTTCTTCTGGAAGCCAAACTGCTTTCTCCCACAACAGTGTTGGAGGGTCCTTATGTGGGCAGGTCAAAGCTTCAGTCTTCCTCATTCAGGATGGAGTGTTATGGCGGTTTTAGCCCTGAGAGAGGAGGGGAGCTTGGTGCTCTGGTTCTGAGAAGGAAAGGGAAGGAGAGAGGGTGGTTTGACTCATGGGGGAGGAGGGTGTTGAAGGGAAAAAGAGAGGTTGGTGGGGCTAGTTATGTCTTTCCATCTTCTGTGGATGGAGAGACTGACGGTGGTGGCTGTGTGGGAGGAGAGAGTGGGAGGTTGGAGATGAAGAAGAAGACGACAGGGATTACAAGGGTTGGGAGCTTTTCTAGTCTCTCTCATGCCAAGCCTCATTTCTGGGCAACAATTAAACAGAGCTTGAAGCAGGCAGTTCCATGGAAGAGCAGAAAATTCAAGAAAGATGGGTTTGCGATATGA

Protein Analysis

230

Amino Acids

25.04

Weight (kDa)

9.75

Isoelectric Point (pI)

52.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00950
fragaria_vesca FvH4_7g16850
malus_domestica MD01G1083500.v1.1 MD07G1152200.v1.1
prunus_persica Prupe.2G190500_v2.0.a1
pyrus_communis pycom07g15030
rosa_chinensis RchiOBHm_Chr1g0360071
rosa_laevigata RLG00000027835
rosa_multiflora Rmu_ssc0000368.1_g000038
rosa_roxburghii Rroxscaffold_4G00296400
rosa_rugosa Rorug01G0274700
rosa_samantha Rh1AG288700 Rh1BG254200 Rh1CG271700 Rh1DG283300
rosa_wichuraiana Rw1G025600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 83
AciI CCGC 3 cut(s) 29, 127, 333
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 662
AcuI CTGAAG 2 cut(s) 63, 284
AfiI CCNNNNNNNGG 3 cut(s) 284, 315, 350
AgsI TTSAA 3 cut(s) 433, 634, 667
AjnI CCWGG 1 cut(s) 168
AjuI GAANNNNNNNTTGG 4 cut(s) 181, 213, 590, 622
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 5 cut(s) 217, 298, 361, 576, 630
AluI AGCT 5 cut(s) 217, 298, 361, 576, 630
Alw21I GWGCWC 2 cut(s) 219, 370
Alw26I GTCTC 3 cut(s) 118, 485, 590
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 2 cut(s) 155, 171
ApeKI GCWGC 1 cut(s) 139
ApoI RAATTY 1 cut(s) 662
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspS9I GGNCC 2 cut(s) 156, 275
AsuC2I CCSGG 2 cut(s) 144, 145
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 1 cut(s) 275
BalI TGGCCA 1 cut(s) 173
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 219
BbsI GAAGAC 2 cut(s) 297, 554
Bbv12I GWGCWC 2 cut(s) 219, 370
BbvI GCAGC 1 cut(s) 151
BccI CCATC 4 cut(s) 313, 479, 481, 668
BciT130I CCWGG 1 cut(s) 170
BcnI CCSGG 2 cut(s) 144, 145
BcoDI GTCTC 3 cut(s) 118, 485, 590
BfaI CTAG 2 cut(s) 459, 582
BfuAI ACCTGC 1 cut(s) 279
BisI GCNGC 2 cut(s) 29, 140
BlsI GCNGC 2 cut(s) 30, 141
Bme1390I CCNGG 3 cut(s) 144, 145, 170
Bme18I GGWCC 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 2 cut(s) 156, 275
BmiI GGNNCC 2 cut(s) 85, 276
BmrFI CCNGG 3 cut(s) 144, 145, 170
BpiI GAAGAC 2 cut(s) 297, 554
BpuMI CCSGG 2 cut(s) 144, 145
BsaJI CCNNGG 4 cut(s) 143, 152, 224, 647
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 386, 416
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 315, 350
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 4 cut(s) 143, 152, 224, 647
BseGI GGATG 2 cut(s) 324, 490
BseLI CCNNNNNNNGG 3 cut(s) 284, 315, 350
BseMII CTCAG 4 cut(s) 123, 129, 336, 368
BseRI GAGGAG 3 cut(s) 366, 436, 531
BseXI GCAGC 1 cut(s) 151
BseYI CCCAGC 2 cut(s) 7, 129
BsgI GTGCAG 1 cut(s) 145
BshFI GGCC 2 cut(s) 157, 173
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 2 cut(s) 219, 370
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 3 cut(s) 284, 315, 350
BsmAI GTCTC 3 cut(s) 118, 485, 590
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 2 cut(s) 157, 173
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 219, 370
Bsp143I GATC 1 cut(s) 11
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 3 cut(s) 29, 127, 333
BspANI GGCC 2 cut(s) 157, 173
BspCNI CTCAG 4 cut(s) 122, 128, 337, 369
BspLI GGNNCC 2 cut(s) 85, 276
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 2 cut(s) 129, 647
BspT107I GGYRCC 1 cut(s) 83
BssECI CCNNGG 4 cut(s) 143, 152, 224, 647
BssMI GATC 1 cut(s) 11
BssT1I CCWWGG 3 cut(s) 152, 224, 647
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 166, 265, 500
Bst6I CTCTTC 2 cut(s) 129, 647
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 5 cut(s) 109, 115, 211, 345, 377
BstDSI CCRYGG 1 cut(s) 647
BstENI CCTNNNNNAGG 2 cut(s) 313, 348
BstF5I GGATG 2 cut(s) 324, 490
BstKTI GATC 1 cut(s) 14
BstMAI GTCTC 3 cut(s) 118, 485, 590
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 3 cut(s) 37, 148, 339
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 3 cut(s) 142, 143, 168
BstV1I GCAGC 1 cut(s) 151
BstV2I GAAGAC 2 cut(s) 297, 554
BstX2I RGATCY 1 cut(s) 11
BstXI CCANNNNNNTGG 1 cut(s) 129
BstYI RGATCY 1 cut(s) 11
BsuRI GGCC 2 cut(s) 157, 173
BtgI CCRYGG 1 cut(s) 647
BtsCI GGATG 2 cut(s) 324, 490
BtsIMutI CAGTG 2 cut(s) 162, 270
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 639
Cfr13I GGNCC 2 cut(s) 156, 275
Cfr9I CCCGGG 1 cut(s) 143
CviAII CATG 4 cut(s) 71, 414, 593, 648
DdeI CTNAG 5 cut(s) 109, 115, 211, 345, 377
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 2 cut(s) 129, 647
EarI CTCTTC 2 cut(s) 129, 647
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 152, 224, 647
Eco24I GRGCYC 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 63, 284
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 217
EcoNI CCTNNNNNAGG 2 cut(s) 313, 348
EcoO109I RGGNCCY 2 cut(s) 156, 275
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 152, 224, 647
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 3 cut(s) 152, 224, 647
FaeI CATG 4 cut(s) 74, 417, 596, 651
FaiI YATR 8 cut(s) 72, 282, 330, 415, 465, 594, 649, 688
FaqI GGGAC 1 cut(s) 104
FatI CATG 4 cut(s) 70, 413, 592, 647
Fnu4HI GCNGC 2 cut(s) 29, 140
FokI GGATG 2 cut(s) 331, 497
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 2 cut(s) 29, 140
FspBI CTAG 2 cut(s) 459, 582
GluI GCNGC 2 cut(s) 29, 140
GsaI CCCAGC 2 cut(s) 11, 133
HaeIII GGCC 2 cut(s) 157, 173
HapII CCGG 1 cut(s) 144
Hin1II CATG 4 cut(s) 74, 417, 596, 651
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 1 cut(s) 410
HpaII CCGG 1 cut(s) 144
Hpy188I TCNGA 2 cut(s) 16, 378
Hpy188III TCNNGA 4 cut(s) 201, 236, 316, 667
HpyAV CCTTC 4 cut(s) 87, 375, 385, 427
HpyCH4III ACNGT 3 cut(s) 166, 265, 500
HpyCH4V TGCA 1 cut(s) 162
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 148, 339
HpyF3I CTNAG 5 cut(s) 109, 115, 211, 345, 377
Hsp92II CATG 4 cut(s) 74, 417, 596, 651
Kzo9I GATC 1 cut(s) 11
LguI GCTCTTC 2 cut(s) 129, 647
LmnI GCTCC 3 cut(s) 222, 358, 573
Lsp1109I GCAGC 1 cut(s) 151
MaeI CTAG 2 cut(s) 459, 582
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 8 cut(s) 146, 224, 297, 468, 553, 556, 559, 664
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 2 cut(s) 219, 370
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 2 cut(s) 618, 662
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 249, 513
Mox20I TGGCCA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 173
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 2 cut(s) 69, 263
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 3 cut(s) 144, 145, 170
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 37, 148, 339
NciI CCSGG 2 cut(s) 144, 145
NcoI CCATGG 1 cut(s) 647
NdeII GATC 1 cut(s) 11
NlaIII CATG 4 cut(s) 74, 417, 596, 651
NlaIV GGNNCC 2 cut(s) 85, 276
OliI CACNNNNGTG 1 cut(s) 263
PciSI GCTCTTC 2 cut(s) 129, 647
PkrI GCNGC 2 cut(s) 30, 141
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PpuMI RGGWCCY 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 2 cut(s) 7, 129
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 85, 276
PspPI GGNCC 2 cut(s) 156, 275
PspPPI RGGWCCY 1 cut(s) 275
PsuI RGATCY 1 cut(s) 11
RseI CAYNNNNRTG 2 cut(s) 69, 263
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 2 cut(s) 129, 647
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 2 cut(s) 29, 140
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 2 cut(s) 156, 275
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 144, 145, 170
SduI GDGCHC 2 cut(s) 219, 370
SetI ASST 9 cut(s) 110, 219, 293, 300, 363, 450, 533, 578, 632
SinI GGWCC 1 cut(s) 275
SmaI CCCGGG 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 69, 263
Sse9I AATT 2 cut(s) 618, 662
SsiI CCGC 3 cut(s) 29, 127, 333
SspMI CTAG 2 cut(s) 459, 582
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 142, 143, 168
StyI CCWWGG 3 cut(s) 152, 224, 647
TaaI ACNGT 3 cut(s) 166, 265, 500
TasI AATT 2 cut(s) 618, 662
TauI GCSGC 1 cut(s) 31
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 2 cut(s) 169, 270
TseI GCWGC 1 cut(s) 139
TspDTI ATGAA 1 cut(s) 554
TspMI CCCGGG 1 cut(s) 143
TspRI CASTG 2 cut(s) 169, 270
VpaK11BI GGWCC 1 cut(s) 275
XagI CCTNNNNNAGG 2 cut(s) 313, 348
XapI RAATTY 1 cut(s) 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XmaI CCCGGG 1 cut(s) 143
XspI CTAG 2 cut(s) 459, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.