MD01G1083500.v1.1

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014982
Physical Location & Seq
Forward (+)
18998778 .. 19000700
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1083500.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGGGCTGGGATCTGATGAGGCCGAGCCACCACAGCTGCCCTTATTTTCTGCCCCACCATTTCACTCCCACGAGCCATCAGGGACCCTAACCCCGCCCCACCCCTCCTCAGTCTCGGTCCCATTCCGCTGGGAAGAGCAGCCCGGGAAGCCAAGGCCCTGCACTGCCCTGGCCACCCTTCAAAACCCCACTGACTTTTCCCAAAAGTGCTTAGAGCTCCCTCCAAGGCTGCTTTTGGAAGCCAAACAGCTTTCTCCCACCACAGTGTTGGAGGGTCCTTATGTCGGCAGGCCAAGGTTTCAGTCTTCCTCATTCAGGATGGAGTGTTATGGCGGTTTTAGCCCCGAGAGAGGAGGGGGGCTTGGTGCTCTGGTTCTGAGCAGGAAAGGGAAGGAGAGAGGGTGGTTTGACTCATGGGGGAGGAGGGTGTTGAAGGGAAAAAGAGACGTTGGTGGGGCTAGTTATGTCTTTCCATCTTCTGTGGACGGAGAGAGTGACGGTGGTGGCAGTGTGGGAGGAGAGAGTGGGAGGATGAAGCTGAAGAAGAAGGTGCCAAGGATTACAAGGGTTGGGAGCTTTTCTAGTCTCTCTCATGACAAACCTCACTTCTGGGCGACAGTGAAACAGAGCTTGAAACAGGCAGTTCCATGGAAGAATAGAAAATTCAAGAAAGATGGGTTTGTTATATGA

Protein Analysis

230

Amino Acids

25.06

Weight (kDa)

9.89

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00950
fragaria_vesca FvH4_7g16850
malus_domestica MD01G1083500.v1.1 MD07G1152200.v1.1
prunus_persica Prupe.2G190500_v2.0.a1
pyrus_communis pycom07g15030
rosa_chinensis RchiOBHm_Chr1g0360071
rosa_laevigata RLG00000027835
rosa_multiflora Rmu_ssc0000368.1_g000038
rosa_roxburghii Rroxscaffold_4G00296400
rosa_rugosa Rorug01G0274700
rosa_samantha Rh1AG288700 Rh1BG254200 Rh1CG271700 Rh1DG283300
rosa_wichuraiana Rw1G025600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 550
AciI CCGC 3 cut(s) 95, 127, 333
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 662
AcuI CTGAAG 1 cut(s) 560
AfiI CCNNNNNNNGG 3 cut(s) 284, 315, 350
AgsI TTSAA 4 cut(s) 182, 433, 634, 667
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 6 cut(s) 37, 217, 250, 538, 576, 630
AluI AGCT 6 cut(s) 37, 217, 250, 538, 576, 630
Alw21I GWGCWC 2 cut(s) 219, 370
Alw26I GTCTC 3 cut(s) 118, 438, 590
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 2 cut(s) 143, 344
AoxI GGCC 4 cut(s) 21, 155, 171, 290
ApeKI GCWGC 3 cut(s) 37, 139, 229
ApoI RAATTY 1 cut(s) 662
AspS9I GGNCC 4 cut(s) 84, 118, 156, 275
AsuC2I CCSGG 2 cut(s) 144, 145
AvaI CYCGRG 2 cut(s) 143, 344
AvaII GGWCC 3 cut(s) 84, 118, 275
BalI TGGCCA 1 cut(s) 173
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 219
BauI CACGAG 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 219, 370
BbvI GCAGC 3 cut(s) 24, 151, 216
BccI CCATC 4 cut(s) 85, 313, 481, 668
BciT130I CCWGG 1 cut(s) 170
BcnI CCSGG 2 cut(s) 144, 145
BcoDI GTCTC 3 cut(s) 118, 438, 590
BfaI CTAG 2 cut(s) 459, 582
BisI GCNGC 3 cut(s) 38, 140, 230
BlsI GCNGC 3 cut(s) 39, 141, 231
Bme1390I CCNGG 3 cut(s) 144, 145, 170
Bme18I GGWCC 3 cut(s) 84, 118, 275
BmeT110I CYCGRG 2 cut(s) 143, 344
BmgT120I GGNCC 4 cut(s) 84, 118, 156, 275
BmiI GGNNCC 5 cut(s) 85, 86, 120, 276, 552
BmrFI CCNGG 3 cut(s) 144, 145, 170
BpiI GAAGAC 1 cut(s) 297
BpuMI CCSGG 2 cut(s) 144, 145
BsaJI CCNNGG 7 cut(s) 143, 152, 168, 224, 293, 554, 647
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 315, 350
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 7 cut(s) 143, 152, 168, 224, 293, 554, 647
BseGI GGATG 2 cut(s) 324, 537
BseLI CCNNNNNNNGG 3 cut(s) 284, 315, 350
BseMII CTCAG 2 cut(s) 123, 368
BseRI GAGGAG 4 cut(s) 97, 366, 436, 531
BseXI GCAGC 3 cut(s) 24, 151, 216
BseYI CCCAGC 2 cut(s) 7, 129
BsgI GTGCAG 1 cut(s) 145
BshFI GGCC 4 cut(s) 23, 157, 173, 292
BshNI GGYRCC 1 cut(s) 550
BsiHKAI GWGCWC 2 cut(s) 219, 370
BsiHKCI CYCGRG 2 cut(s) 143, 344
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 2 cut(s) 97, 104
BslI CCNNNNNNNGG 3 cut(s) 284, 315, 350
BsmAI GTCTC 3 cut(s) 118, 438, 590
BsmBI CGTCTC 1 cut(s) 438
BsmFI GGGAC 2 cut(s) 97, 104
BsnI GGCC 4 cut(s) 23, 157, 173, 292
BsoBI CYCGRG 2 cut(s) 143, 344
Bsp1286I GDGCHC 2 cut(s) 219, 370
Bsp143I GATC 1 cut(s) 11
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 3 cut(s) 95, 127, 333
BspANI GGCC 4 cut(s) 23, 157, 173, 292
BspCNI CTCAG 2 cut(s) 122, 369
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 5 cut(s) 85, 86, 120, 276, 552
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 550
BssECI CCNNGG 7 cut(s) 143, 152, 168, 224, 293, 554, 647
BssMI GATC 1 cut(s) 11
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 5 cut(s) 152, 224, 293, 554, 647
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 265, 500, 619
Bst6I CTCTTC 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 290
BstDEI CTNAG 3 cut(s) 109, 211, 377
BstDSI CCRYGG 1 cut(s) 647
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 2 cut(s) 324, 537
BstKTI GATC 1 cut(s) 14
BstMAI GTCTC 3 cut(s) 118, 438, 590
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 3 cut(s) 34, 148, 339
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 3 cut(s) 142, 143, 168
BstV1I GCAGC 3 cut(s) 24, 151, 216
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 11
BstXI CCANNNNNNTGG 2 cut(s) 129, 268
BstYI RGATCY 1 cut(s) 11
BsuRI GGCC 4 cut(s) 23, 157, 173, 292
BtgI CCRYGG 1 cut(s) 647
BtsCI GGATG 2 cut(s) 324, 537
BtsI GCAGTG 2 cut(s) 162, 514
BtsIMutI CAGTG 5 cut(s) 162, 189, 270, 514, 624
Cac8I GCNNGC 1 cut(s) 290
CciI TCATGA 1 cut(s) 592
Cfr13I GGNCC 4 cut(s) 84, 118, 156, 275
Cfr9I CCCGGG 1 cut(s) 143
CviAII CATG 3 cut(s) 414, 593, 648
DdeI CTNAG 3 cut(s) 109, 211, 377
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 5 cut(s) 152, 224, 293, 554, 647
Eco24I GRGCYC 1 cut(s) 219
Eco47I GGWCC 3 cut(s) 84, 118, 275
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 560
Eco88I CYCGRG 2 cut(s) 143, 344
EcoICRI GAGCTC 1 cut(s) 217
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoO109I RGGNCCY 3 cut(s) 84, 156, 275
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 5 cut(s) 152, 224, 293, 554, 647
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 5 cut(s) 152, 224, 293, 554, 647
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 3 cut(s) 417, 596, 651
FaiI YATR 8 cut(s) 282, 330, 415, 465, 594, 649, 686, 688
FaqI GGGAC 2 cut(s) 97, 104
FatI CATG 3 cut(s) 413, 592, 647
FauI CCCGC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 38, 140, 230
FokI GGATG 2 cut(s) 331, 544
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 3 cut(s) 38, 140, 230
FspBI CTAG 2 cut(s) 459, 582
GluI GCNGC 3 cut(s) 38, 140, 230
GsaI CCCAGC 2 cut(s) 11, 133
HaeIII GGCC 4 cut(s) 23, 157, 173, 292
HapII CCGG 1 cut(s) 144
Hin1II CATG 3 cut(s) 417, 596, 651
HinfI GANTC 1 cut(s) 410
HpaII CCGG 1 cut(s) 144
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 2 cut(s) 16, 378
Hpy188III TCNNGA 3 cut(s) 316, 593, 667
Hpy8I GTNNAC 1 cut(s) 484
HpyAV CCTTC 4 cut(s) 188, 385, 427, 541
HpyCH4III ACNGT 3 cut(s) 265, 500, 619
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 1 cut(s) 162
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 148, 339
HpyF3I CTNAG 3 cut(s) 109, 211, 377
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 3 cut(s) 417, 596, 651
KflI GGGWCCC 1 cut(s) 84
Kzo9I GATC 1 cut(s) 11
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 2 cut(s) 222, 573
Lsp1109I GCAGC 3 cut(s) 24, 151, 216
MaeI CTAG 2 cut(s) 459, 582
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 494
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 6 cut(s) 146, 297, 468, 553, 556, 664
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 2 cut(s) 219, 370
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 1 cut(s) 662
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 249
Mox20I TGGCCA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 263
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 2 cut(s) 37, 129
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 3 cut(s) 144, 145, 170
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 34, 148, 339
NciI CCSGG 2 cut(s) 144, 145
NcoI CCATGG 1 cut(s) 647
NdeII GATC 1 cut(s) 11
NlaIII CATG 3 cut(s) 417, 596, 651
NlaIV GGNNCC 5 cut(s) 85, 86, 120, 276, 552
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 1 cut(s) 494
OliI CACNNNNGTG 1 cut(s) 263
PagI TCATGA 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 129
PkrI GCNGC 3 cut(s) 39, 141, 231
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PpuMI RGGWCCY 2 cut(s) 84, 275
Psp124BI GAGCTC 1 cut(s) 219
Psp5II RGGWCCY 2 cut(s) 84, 275
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 2 cut(s) 7, 129
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 5 cut(s) 85, 86, 120, 276, 552
PspPI GGNCC 4 cut(s) 84, 118, 156, 275
PspPPI RGGWCCY 2 cut(s) 84, 275
PsuI RGATCY 1 cut(s) 11
PvuII CAGCTG 1 cut(s) 37
RseI CAYNNNNRTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 129
SatI GCNGC 3 cut(s) 38, 140, 230
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 4 cut(s) 84, 118, 156, 275
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 144, 145, 170
SduI GDGCHC 2 cut(s) 219, 370
SinI GGWCC 3 cut(s) 84, 118, 275
SmaI CCCGGG 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 1 cut(s) 662
SsiI CCGC 3 cut(s) 95, 127, 333
SspMI CTAG 2 cut(s) 459, 582
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 142, 143, 168
StyI CCWWGG 5 cut(s) 152, 224, 293, 554, 647
TaaI ACNGT 3 cut(s) 265, 500, 619
TaiI ACGT 1 cut(s) 450
TaqII GACCGA 1 cut(s) 106
TasI AATT 1 cut(s) 662
TscAI CASTG 5 cut(s) 169, 196, 270, 514, 624
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 3 cut(s) 37, 139, 229
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 1 cut(s) 548
TspGWI ACGGA 1 cut(s) 501
TspMI CCCGGG 1 cut(s) 143
TspRI CASTG 5 cut(s) 169, 196, 270, 514, 624
VpaK11BI GGWCC 3 cut(s) 84, 118, 275
XagI CCTNNNNNAGG 1 cut(s) 313
XapI RAATTY 1 cut(s) 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XmaI CCCGGG 1 cut(s) 143
XspI CTAG 2 cut(s) 459, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.