Rorug01G0274700

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014982
Physical Location & Seq
Reverse (-)
38695266 .. 38696597
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0274700.1

Sequence Viewer

Length: 885 bp
ATGTTCCCCAGCACTACTAAACGATCGAGCAAAACCCTGATGGCCCCAAGAGTTGGAACCCACGTAAAACGATGCCTGAGCCGCCGAGACTGGACGGAACTTCCGGGCGTCGTTACGGCATCGATACTGTCACATCTCGGAGACATCGAGTCCCTGACGGTGGCTGAGATGGTGTGCAAGACGTGGCGTGAAATCTGCAAAGACCCTATGCTGTGGCGCACCATCGACTTCATGAGCAACCATACAAAGTTCATGTTCGGGCCGCCGTTGCGTTACGATATAGTGAAGATGTGCCGCCGCGCCATACACCGTAGCTCCGGTAGTTTTGTCTATGTCAACGTCGACTACTTTTGCAACAACGAACTGTTAAAGTATATCACCAACAGTTCAGCTGGATTTATTAGACACCTCAGTTTGTTCAATTGTGGCATTATAACTGATGAGGTGTTGTGTGAAGCAGCTTTGAGACTTCCAGTTTTGGAGGAACTTGACATTTCGATGTGCAAATTCTCCAGTAAAGCTCTACAAGTAGTTGGGCGCTGCTGCCCTCTTTTGAAATCATTCAAATTTAACACCCCGAATGGAAGAAAGTCTTCCAGGCAGTATTATAGCGACGAGGATGCAGTTGCTATATCAGGAACCATGCATGGTTTAGTCCTCCTTGAGATCCGTAGGAATGAGCTGACTTGCAATGGCTTGGAGACAATTCTTGATTGTTGTCCTCAACTCGAGATCCTTGATTTGCGCAAGTGTAAAAACCTGAGATATATGAGCACAGATTTAGAAATAAGATGTGTTGAAAGAATTAAGAAGTTGTGGCTCCGCGGCCCGATCGATCCCTCTAGTATATCGGACTTGAATTTGGTGGGCAAACCATGTTATTAA

Protein Analysis

294

Amino Acids

33.57

Weight (kDa)

8.9

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 31 - 77 1e-10 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00950
fragaria_vesca FvH4_7g16850
malus_domestica MD01G1083500.v1.1 MD07G1152200.v1.1
prunus_persica Prupe.2G190500_v2.0.a1
pyrus_communis pycom07g15030
rosa_chinensis RchiOBHm_Chr1g0360071
rosa_laevigata RLG00000027835
rosa_multiflora Rmu_ssc0000368.1_g000038
rosa_roxburghii Rroxscaffold_4G00296400
rosa_rugosa Rorug01G0274700
rosa_samantha Rh1AG288700 Rh1BG254200 Rh1CG271700 Rh1DG283300
rosa_wichuraiana Rw1G025600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
Acc16I TGCGCA 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 53
AccI GTMKAC 1 cut(s) 342
AccII CGCG 2 cut(s) 300, 825
AciI CCGC 6 cut(s) 82, 263, 295, 298, 823, 825
AclWI GGATC 3 cut(s) 661, 727, 830
AcsI RAATTY 3 cut(s) 506, 566, 859
AcyI GRCGYC 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 53, 160
AgsI TTSAA 5 cut(s) 421, 556, 565, 800, 859
AhdI GACNNNNNGTC 1 cut(s) 148
AjiI CACGTC 1 cut(s) 183
AjnI CCWGG 1 cut(s) 596
AluBI AGCT 5 cut(s) 315, 392, 461, 521, 682
AluI AGCT 5 cut(s) 315, 392, 461, 521, 682
Alw21I GWGCWC 1 cut(s) 776
Alw26I GTCTC 4 cut(s) 81, 135, 460, 695
AlwI GGATC 3 cut(s) 661, 727, 830
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 3 cut(s) 42, 260, 826
ApeKI GCWGC 3 cut(s) 458, 540, 543
ApoI RAATTY 3 cut(s) 506, 566, 859
Asp700I GAANNNNTTC 2 cut(s) 560, 592
AspLEI GCGC 4 cut(s) 219, 302, 540, 747
AspS9I GGNCC 3 cut(s) 43, 260, 827
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 728
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 776
BbvI GCAGC 3 cut(s) 470, 527, 530
BccI CCATC 3 cut(s) 34, 163, 230
BceAI ACGGC 2 cut(s) 132, 250
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 1 cut(s) 598
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 4 cut(s) 81, 135, 460, 695
BfaI CTAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 541
BisI GCNGC 8 cut(s) 82, 263, 295, 298, 459, 541, 544, 826
BlsI GCNGC 8 cut(s) 83, 264, 296, 299, 460, 542, 545, 827
Bme1390I CCNGG 2 cut(s) 105, 598
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 183
BmgT120I GGNCC 3 cut(s) 43, 260, 827
BmiI GGNNCC 4 cut(s) 45, 58, 640, 821
BmrFI CCNGG 2 cut(s) 105, 598
BmsI GCATC 3 cut(s) 62, 128, 610
BpiI GAAGAC 1 cut(s) 585
BpmI CTGGAG 1 cut(s) 496
Bpu10I CCTNAGC 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 105
Bsa29I ATCGAT 2 cut(s) 122, 834
BsaAI YACGTR 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 823
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 160
Bse1I ACTGG 3 cut(s) 95, 473, 513
Bse3DI GCAATG 1 cut(s) 697
BseBI CCWGG 1 cut(s) 598
BseCI ATCGAT 2 cut(s) 122, 834
BseDI CCNNGG 1 cut(s) 823
BseGI GGATG 1 cut(s) 625
BseLI CCNNNNNNNGG 2 cut(s) 53, 160
BseMI GCAATG 1 cut(s) 697
BseMII CTCAG 4 cut(s) 68, 156, 424, 752
BseNI ACTGG 3 cut(s) 95, 473, 513
BseXI GCAGC 3 cut(s) 470, 527, 530
BseYI CCCAGC 1 cut(s) 8
Bsh1236I CGCG 2 cut(s) 300, 825
Bsh1285I CGRYCG 2 cut(s) 26, 834
BshFI GGCC 3 cut(s) 44, 262, 828
BshVI ATCGAT 2 cut(s) 122, 834
BsiEI CGRYCG 2 cut(s) 26, 834
BsiHKAI GWGCWC 1 cut(s) 776
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 2 cut(s) 104, 318
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 2 cut(s) 53, 160
BsmAI GTCTC 4 cut(s) 81, 135, 460, 695
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 3 cut(s) 44, 262, 828
BsoBI CYCGRG 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 776
Bsp143I GATC 5 cut(s) 23, 666, 732, 831, 835
BspACI CCGC 6 cut(s) 82, 263, 295, 298, 823, 825
BspANI GGCC 3 cut(s) 44, 262, 828
BspCNI CTCAG 4 cut(s) 69, 157, 423, 753
BspDI ATCGAT 2 cut(s) 122, 834
BspFNI CGCG 2 cut(s) 300, 825
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 4 cut(s) 45, 58, 640, 821
BspPI GGATC 3 cut(s) 661, 727, 830
BsrDI GCAATG 1 cut(s) 697
BsrI ACTGG 3 cut(s) 95, 473, 513
BssECI CCNNGG 1 cut(s) 823
BssMI GATC 5 cut(s) 23, 666, 732, 831, 835
BssNI GRCGYC 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 598
Bst4CI ACNGT 5 cut(s) 129, 160, 311, 366, 386
BstACI GRCGYC 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 64
BstDEI CTNAG 4 cut(s) 77, 165, 410, 761
BstDSI CCRYGG 1 cut(s) 823
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 2 cut(s) 300, 825
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 4 cut(s) 219, 302, 540, 747
BstKTI GATC 5 cut(s) 26, 669, 735, 834, 838
BstMAI GTCTC 4 cut(s) 81, 135, 460, 695
BstMBI GATC 5 cut(s) 23, 666, 732, 831, 835
BstMCI CGRYCG 2 cut(s) 26, 834
BstMWI GCNNNNNNNGC 2 cut(s) 81, 268
BstNI CCWGG 1 cut(s) 598
BstSCI CCNGG 2 cut(s) 103, 596
BstUI CGCG 2 cut(s) 300, 825
BstV1I GCAGC 3 cut(s) 470, 527, 530
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 2 cut(s) 666, 732
BstYI RGATCY 2 cut(s) 666, 732
Bsu15I ATCGAT 2 cut(s) 122, 834
BsuRI GGCC 3 cut(s) 44, 262, 828
BsuTUI ATCGAT 2 cut(s) 122, 834
BtgI CCRYGG 1 cut(s) 823
BtrI CACGTC 1 cut(s) 183
BtsCI GGATG 1 cut(s) 625
CciI TCATGA 1 cut(s) 231
CfoI GCGC 4 cut(s) 219, 302, 540, 747
Cfr13I GGNCC 3 cut(s) 43, 260, 827
Cfr42I CCGCGG 1 cut(s) 826
ClaI ATCGAT 2 cut(s) 122, 834
CseI GACGC 1 cut(s) 97
CviAII CATG 5 cut(s) 232, 253, 643, 647, 876
DdeI CTNAG 4 cut(s) 77, 165, 410, 761
DpnI GATC 5 cut(s) 25, 668, 734, 833, 837
DpnII GATC 5 cut(s) 23, 666, 732, 831, 835
DriI GACNNNNNGTC 1 cut(s) 148
Eam1105I GACNNNNNGTC 1 cut(s) 148
Eco88I CYCGRG 1 cut(s) 728
EcoRII CCWGG 1 cut(s) 596
EcoT22I ATGCAT 1 cut(s) 648
FaeI CATG 5 cut(s) 235, 256, 646, 650, 879
FalI AAGNNNNNCTT 2 cut(s) 577, 609
FaqI GGGAC 1 cut(s) 136
FatI CATG 5 cut(s) 231, 252, 642, 646, 875
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 8 cut(s) 82, 263, 295, 298, 459, 541, 544, 826
FokI GGATG 1 cut(s) 632
Fsp4HI GCNGC 8 cut(s) 82, 263, 295, 298, 459, 541, 544, 826
FspBI CTAG 1 cut(s) 843
FspI TGCGCA 1 cut(s) 746
GlaI GCGC 4 cut(s) 218, 301, 539, 746
GluI GCNGC 8 cut(s) 82, 263, 295, 298, 459, 541, 544, 826
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 496
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 3 cut(s) 44, 262, 828
HapII CCGG 2 cut(s) 104, 318
HgaI GACGC 1 cut(s) 97
HhaI GCGC 4 cut(s) 219, 302, 540, 747
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 5 cut(s) 235, 256, 646, 650, 879
Hin6I GCGC 4 cut(s) 217, 300, 538, 745
HinP1I GCGC 4 cut(s) 217, 300, 538, 745
HincII GTYRAC 2 cut(s) 337, 343
HindII GTYRAC 2 cut(s) 337, 343
HinfI GANTC 1 cut(s) 149
HpaII CCGG 2 cut(s) 104, 318
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 2 cut(s) 337, 343
Hpy188I TCNGA 2 cut(s) 140, 853
Hpy188III TCNNGA 4 cut(s) 232, 636, 710, 730
Hpy8I GTNNAC 2 cut(s) 337, 343
Hpy99I CGWCG 3 cut(s) 113, 344, 617
HpyCH4III ACNGT 5 cut(s) 129, 160, 311, 366, 386
HpyCH4IV ACGT 3 cut(s) 63, 182, 339
HpyCH4V TGCA 7 cut(s) 177, 198, 354, 504, 623, 646, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 268
HpyF3I CTNAG 4 cut(s) 77, 165, 410, 761
HpySE526I ACGT 3 cut(s) 63, 182, 339
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 5 cut(s) 235, 256, 646, 650, 879
HspAI GCGC 4 cut(s) 217, 300, 538, 745
KspI CCGCGG 1 cut(s) 826
Kzo9I GATC 5 cut(s) 23, 666, 732, 831, 835
LmnI GCTCC 2 cut(s) 320, 825
Lsp1109I GCAGC 3 cut(s) 470, 527, 530
LweI GCATC 3 cut(s) 62, 128, 610
MaeI CTAG 1 cut(s) 843
MaeII ACGT 3 cut(s) 63, 182, 339
MaeIII GTNAC 3 cut(s) 112, 129, 272
MalI GATC 5 cut(s) 25, 668, 734, 833, 837
MboI GATC 5 cut(s) 23, 666, 732, 831, 835
MboII GAAGA 3 cut(s) 298, 585, 597
MfeI CAATTG 1 cut(s) 421
MflI RGATCY 2 cut(s) 666, 732
MhlI GDGCHC 1 cut(s) 776
MluCI AATT 6 cut(s) 421, 506, 566, 705, 804, 859
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 8 cut(s) 419, 436, 475, 558, 610, 668, 732, 850
Mph1103I ATGCAT 1 cut(s) 648
MroXI GAANNNNTTC 2 cut(s) 560, 592
MseI TTAA 4 cut(s) 368, 570, 807, 883
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 2 cut(s) 392, 825
MspI CCGG 2 cut(s) 104, 318
MspR9I CCNGG 2 cut(s) 105, 598
MunI CAATTG 1 cut(s) 421
MvaI CCWGG 1 cut(s) 598
MvnI CGCG 2 cut(s) 300, 825
MwoI GCNNNNNNNGC 2 cut(s) 81, 268
NciI CCSGG 1 cut(s) 105
NdeII GATC 5 cut(s) 23, 666, 732, 831, 835
NlaIII CATG 5 cut(s) 235, 256, 646, 650, 879
NlaIV GGNNCC 4 cut(s) 45, 58, 640, 821
NmeAIII GCCGAG 1 cut(s) 110
NmuCI GTSAC 1 cut(s) 129
NsbI TGCGCA 1 cut(s) 746
NsiI ATGCAT 1 cut(s) 648
PaeR7I CTCGAG 1 cut(s) 728
PagI TCATGA 1 cut(s) 231
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PdmI GAANNNNTTC 2 cut(s) 560, 592
PflMI CCANNNNNTGG 1 cut(s) 53
PkrI GCNGC 8 cut(s) 83, 264, 296, 299, 460, 542, 545, 827
Ple19I CGATCG 2 cut(s) 26, 834
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
Ppu21I YACGTR 1 cut(s) 64
PsiI TTATAA 1 cut(s) 434
Psp6I CCWGG 1 cut(s) 596
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 1 cut(s) 596
PspN4I GGNNCC 4 cut(s) 45, 58, 640, 821
PspPI GGNCC 3 cut(s) 43, 260, 827
PsuI RGATCY 2 cut(s) 666, 732
PvuI CGATCG 2 cut(s) 26, 834
PvuII CAGCTG 1 cut(s) 392
RseI CAYNNNNRTG 1 cut(s) 497
SacII CCGCGG 1 cut(s) 826
SalI GTCGAC 1 cut(s) 341
SaqAI TTAA 4 cut(s) 368, 570, 807, 883
SatI GCNGC 8 cut(s) 82, 263, 295, 298, 459, 541, 544, 826
Sau3AI GATC 5 cut(s) 23, 666, 732, 831, 835
Sau96I GGNCC 3 cut(s) 43, 260, 827
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 105, 598
SduI GDGCHC 1 cut(s) 776
SfaNI GCATC 3 cut(s) 62, 128, 610
Sfr274I CTCGAG 1 cut(s) 728
Sfr303I CCGCGG 1 cut(s) 826
SgrBI CCGCGG 1 cut(s) 826
SlaI CTCGAG 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 662, 728
SmoI CTYRAG 2 cut(s) 662, 728
Sse9I AATT 6 cut(s) 421, 506, 566, 705, 804, 859
SsiI CCGC 6 cut(s) 82, 263, 295, 298, 823, 825
SspMI CTAG 1 cut(s) 843
StyD4I CCNGG 2 cut(s) 103, 596
TaaI ACNGT 5 cut(s) 129, 160, 311, 366, 386
TaiI ACGT 3 cut(s) 66, 185, 342
TaqI TCGA 8 cut(s) 26, 122, 147, 225, 342, 497, 729, 834
TasI AATT 6 cut(s) 421, 506, 566, 705, 804, 859
TauI GCSGC 5 cut(s) 84, 265, 297, 300, 828
Tru1I TTAA 4 cut(s) 368, 570, 807, 883
Tru9I TTAA 4 cut(s) 368, 570, 807, 883
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 3 cut(s) 458, 540, 543
Tsp45I GTSAC 1 cut(s) 129
TspDTI ATGAA 2 cut(s) 220, 241
TspGWI ACGGA 2 cut(s) 110, 659
Van91I CCANNNNNTGG 1 cut(s) 53
XapI RAATTY 3 cut(s) 506, 566, 859
XhoI CTCGAG 1 cut(s) 728
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 2 cut(s) 560, 592
XspI CTAG 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.