MD07G1152200.v1.1

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014982
Physical Location & Seq
Forward (+)
22188821 .. 22191047
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1152200.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGGGCTGGGATCTGATGAGGCAGAGCCACCACAACTGCCCTTATTTTCTGCCCCACCATTTCACTCCCATGAGCCTTCAGGCACCCTAACTCCACCACTCCACACCTCAGTCTCAGTCCCATTCCGCTGGGAAGAGCAGCCCGGGAAGCCAAGGCCCTGCACTGCCCTGGCCACCGTTCCAAACCCAACAGACTTTTCCCGAAAGTGCTTAGAGCTCCCTCCAAGGCTGCTTTTGGAAGCCAAACTGCTTTCTCCCACCACAGTGTTGGAGGATCCTTACGTGGGCAGGTCAAAGTTTCAGTCTTCCTCATTCAGGATGGAGTGTTATGGCGGTTTCAGCCCTGAGAGAGGAGGGGAGCTTGGTGCTCTGGTTCTGAGAAGGAAAGGGAAGGAGAGAGGGTGGTTTGACTCATGGGGGAGGAGGGTGTTGAAGGGAAAAAGAGAGGTTGGTGGGGCTGGTTATGTCTTTCCATCTTCTGTGGATGGAGAGAGTGACGGTGGTGGCAGTGTGGGAGGAGAGAGTGGGAAGTTGAAGACGAAGAAGAAGATGACAGGGATTACAACGGTTGGGAGCATTTCTAGTCTCTCTCATGCCAAGCCTCATTTCTGGGCAACAATTAAACAGAGCTTGAAGCAGGCGGCAGTTCCATGGAAGGGTAGAAAATTCAAGAAAGACGGGTTTGCGATATGA

Protein Analysis

231

Amino Acids

24.95

Weight (kDa)

9.76

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00950
fragaria_vesca FvH4_7g16850
malus_domestica MD01G1083500.v1.1 MD07G1152200.v1.1
prunus_persica Prupe.2G190500_v2.0.a1
pyrus_communis pycom07g15030
rosa_chinensis RchiOBHm_Chr1g0360071
rosa_laevigata RLG00000027835
rosa_multiflora Rmu_ssc0000368.1_g000038
rosa_roxburghii Rroxscaffold_4G00296400
rosa_rugosa Rorug01G0274700
rosa_samantha Rh1AG288700 Rh1BG254200 Rh1CG271700 Rh1DG283300
rosa_wichuraiana Rw1G025600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 83
AciI CCGC 3 cut(s) 127, 333, 641
AclWI GGATC 3 cut(s) 19, 269, 282
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 1 cut(s) 63
AfiI CCNNNNNNNGG 4 cut(s) 284, 315, 350, 656
AgsI TTSAA 4 cut(s) 433, 535, 634, 670
AjnI CCWGG 1 cut(s) 168
AjuI GAANNNNNNNTTGG 4 cut(s) 181, 213, 590, 622
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 3 cut(s) 217, 361, 630
AluI AGCT 3 cut(s) 217, 361, 630
Alw21I GWGCWC 2 cut(s) 219, 370
Alw26I GTCTC 2 cut(s) 118, 590
AlwI GGATC 3 cut(s) 19, 269, 282
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 2 cut(s) 155, 171
ApeKI GCWGC 2 cut(s) 139, 229
ApoI RAATTY 1 cut(s) 665
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspS9I GGNCC 1 cut(s) 156
AsuC2I CCSGG 2 cut(s) 144, 145
AvaI CYCGRG 1 cut(s) 143
BalI TGGCCA 1 cut(s) 173
BamHI GGATCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 219
BbsI GAAGAC 2 cut(s) 297, 542
Bbv12I GWGCWC 2 cut(s) 219, 370
BbvI GCAGC 2 cut(s) 151, 216
BccI CCATC 3 cut(s) 313, 479, 481
BciT130I CCWGG 1 cut(s) 170
BcnI CCSGG 2 cut(s) 144, 145
BcoDI GTCTC 2 cut(s) 118, 590
BfaI CTAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 279
BisI GCNGC 3 cut(s) 140, 230, 642
BlsI GCNGC 3 cut(s) 141, 231, 643
Bme1390I CCNGG 3 cut(s) 144, 145, 170
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 2 cut(s) 85, 276
BmrFI CCNGG 3 cut(s) 144, 145, 170
BpiI GAAGAC 2 cut(s) 297, 542
BpuMI CCSGG 2 cut(s) 144, 145
BsaAI YACGTR 1 cut(s) 283
BsaJI CCNNGG 5 cut(s) 143, 152, 168, 224, 650
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 386, 416
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 315, 350, 656
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 5 cut(s) 143, 152, 168, 224, 650
BseGI GGATG 2 cut(s) 324, 490
BseLI CCNNNNNNNGG 4 cut(s) 284, 315, 350, 656
BseMII CTCAG 4 cut(s) 123, 129, 336, 368
BseRI GAGGAG 3 cut(s) 366, 436, 531
BseXI GCAGC 2 cut(s) 151, 216
BseYI CCCAGC 2 cut(s) 7, 129
BsgI GTGCAG 1 cut(s) 145
BshFI GGCC 2 cut(s) 157, 173
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 2 cut(s) 219, 370
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 4 cut(s) 284, 315, 350, 656
BsmAI GTCTC 2 cut(s) 118, 590
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 2 cut(s) 157, 173
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 219, 370
Bsp143I GATC 2 cut(s) 11, 274
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 3 cut(s) 127, 333, 641
BspANI GGCC 2 cut(s) 157, 173
BspCNI CTCAG 4 cut(s) 122, 128, 337, 369
BspLI GGNNCC 2 cut(s) 85, 276
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 3 cut(s) 19, 269, 282
BspQI GCTCTTC 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 83
BssECI CCNNGG 5 cut(s) 143, 152, 168, 224, 650
BssMI GATC 2 cut(s) 11, 274
BssT1I CCWWGG 3 cut(s) 152, 224, 650
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 4 cut(s) 178, 265, 500, 568
Bst6I CTCTTC 1 cut(s) 129
BstBAI YACGTR 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 5 cut(s) 109, 115, 211, 345, 377
BstDSI CCRYGG 1 cut(s) 650
BstENI CCTNNNNNAGG 2 cut(s) 313, 348
BstF5I GGATG 2 cut(s) 324, 490
BstKTI GATC 2 cut(s) 14, 277
BstMAI GTCTC 2 cut(s) 118, 590
BstMBI GATC 2 cut(s) 11, 274
BstMWI GCNNNNNNNGC 2 cut(s) 148, 339
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 3 cut(s) 142, 143, 168
BstV1I GCAGC 2 cut(s) 151, 216
BstV2I GAAGAC 2 cut(s) 297, 542
BstX2I RGATCY 2 cut(s) 11, 274
BstXI CCANNNNNNTGG 2 cut(s) 129, 268
BstYI RGATCY 2 cut(s) 11, 274
BsuRI GGCC 2 cut(s) 157, 173
BtgI CCRYGG 1 cut(s) 650
BtsCI GGATG 2 cut(s) 324, 490
BtsI GCAGTG 2 cut(s) 162, 514
BtsIMutI CAGTG 3 cut(s) 162, 270, 514
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 639
Cfr13I GGNCC 1 cut(s) 156
Cfr9I CCCGGG 1 cut(s) 143
CviAII CATG 4 cut(s) 71, 414, 593, 651
DdeI CTNAG 5 cut(s) 109, 115, 211, 345, 377
DpnI GATC 2 cut(s) 13, 276
DpnII GATC 2 cut(s) 11, 274
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 152, 224, 650
Eco24I GRGCYC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 217
EcoNI CCTNNNNNAGG 2 cut(s) 313, 348
EcoO109I RGGNCCY 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 152, 224, 650
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 3 cut(s) 152, 224, 650
FaeI CATG 4 cut(s) 74, 417, 596, 654
FaiI YATR 7 cut(s) 72, 330, 415, 465, 594, 652, 691
FaqI GGGAC 1 cut(s) 104
FatI CATG 4 cut(s) 70, 413, 592, 650
Fnu4HI GCNGC 3 cut(s) 140, 230, 642
FokI GGATG 2 cut(s) 331, 497
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 3 cut(s) 140, 230, 642
FspBI CTAG 1 cut(s) 582
GluI GCNGC 3 cut(s) 140, 230, 642
GsaI CCCAGC 2 cut(s) 11, 133
HaeIII GGCC 2 cut(s) 157, 173
HapII CCGG 1 cut(s) 144
Hin1II CATG 4 cut(s) 74, 417, 596, 654
HinfI GANTC 1 cut(s) 410
HpaII CCGG 1 cut(s) 144
Hpy188I TCNGA 2 cut(s) 16, 378
Hpy188III TCNNGA 3 cut(s) 201, 316, 670
HpyAV CCTTC 5 cut(s) 87, 375, 385, 427, 649
HpyCH4III ACNGT 4 cut(s) 178, 265, 500, 568
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 1 cut(s) 162
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 339
HpyF3I CTNAG 5 cut(s) 109, 115, 211, 345, 377
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 4 cut(s) 74, 417, 596, 654
Kzo9I GATC 2 cut(s) 11, 274
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 3 cut(s) 222, 358, 573
Lsp1109I GCAGC 2 cut(s) 151, 216
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 494
MalI GATC 2 cut(s) 13, 276
MboI GATC 2 cut(s) 11, 274
MboII GAAGA 7 cut(s) 146, 297, 468, 547, 553, 556, 559
MflI RGATCY 2 cut(s) 11, 274
MhlI GDGCHC 2 cut(s) 219, 370
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 2 cut(s) 618, 665
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 249
Mox20I TGGCCA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 173
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 2 cut(s) 69, 263
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 3 cut(s) 144, 145, 170
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 2 cut(s) 148, 339
NciI CCSGG 2 cut(s) 144, 145
NcoI CCATGG 1 cut(s) 650
NdeII GATC 2 cut(s) 11, 274
NlaIII CATG 4 cut(s) 74, 417, 596, 654
NlaIV GGNNCC 2 cut(s) 85, 276
NmuCI GTSAC 1 cut(s) 494
OliI CACNNNNGTG 1 cut(s) 263
PciSI GCTCTTC 1 cut(s) 129
PkrI GCNGC 3 cut(s) 141, 231, 643
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Ppu21I YACGTR 1 cut(s) 283
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 2 cut(s) 7, 129
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 85, 276
PspPI GGNCC 1 cut(s) 156
PsuI RGATCY 2 cut(s) 11, 274
RseI CAYNNNNRTG 2 cut(s) 69, 263
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 3 cut(s) 140, 230, 642
Sau3AI GATC 2 cut(s) 11, 274
Sau96I GGNCC 1 cut(s) 156
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 144, 145, 170
SduI GDGCHC 2 cut(s) 219, 370
SetI ASST 7 cut(s) 110, 219, 285, 293, 363, 450, 632
SmaI CCCGGG 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 69, 263
Sse9I AATT 2 cut(s) 618, 665
SsiI CCGC 3 cut(s) 127, 333, 641
SspMI CTAG 1 cut(s) 582
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 142, 143, 168
StyI CCWWGG 3 cut(s) 152, 224, 650
TaaI ACNGT 4 cut(s) 178, 265, 500, 568
TaiI ACGT 1 cut(s) 285
TasI AATT 2 cut(s) 618, 665
TauI GCSGC 1 cut(s) 644
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 3 cut(s) 169, 270, 514
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 2 cut(s) 139, 229
Tsp45I GTSAC 1 cut(s) 494
TspMI CCCGGG 1 cut(s) 143
TspRI CASTG 3 cut(s) 169, 270, 514
XagI CCTNNNNNAGG 2 cut(s) 313, 348
XapI RAATTY 1 cut(s) 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XmaI CCCGGG 1 cut(s) 143
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.