Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009544

Physical Location & Seq

Genomic Coordinates Forward (+)

881061 .. 885463

Gene Structure 4403 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G02010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGTCGATTTTCTCACTCGCGAGCTCTTCTGATTCACAAAGAGATGACCTAATAATGGCGTTGAAGGCGGTGGTTATTGTATATTGTGTGGTTTCTCTCGTCAGTGTTCAATTAGCTGATGCTCAACATGAAGGACTGCCAGTTTCACCAACGTTATCACCTTCAACTTCACCAGTTATCACTGATCTGCCCCTACCAGCTGAATTTCCGCGGTTTCACAGAAAGTATTTCGCACCACAACAAGCAGAAGCACCTCAGCATTCTCCCCCTTATAGTCGTTTGGTCGCTTCTGATCATCCCCCTACCAGCTCACATTTCTCCAAACCTTCCATGAAAAGGAATGCTCAGTCTCCTGGAGCCGGCTTGGCTGATATTGCTCCAGCACAATCTAGCAATGGTGTTCTTCCTGATGCCTTAACTCAGCCACCTTTGTCGCCCTCCATTTCAAATTGTTGCAAATCAGATATGGTGCTTAAACGAAGAAGTATTGGTTGCCACTGCGTGTATCCTATAAAACTGGACATCCTTCTCTTGAATGTTTCAGAAACTCCTAGTTGGAACATGTTCTTGAACGAATTTGCTACCCAGCTTGGTCTCCTACCTCACCAAATCGAGCTGATTAACTTCTATGTGCTAAGCTTATCAAGGATGAACATATCGATGGATATCACCCCTCATTCTGGAATTAGTTTCTCAGCTAGTCAGGCATCCGCAATAAACTCTTCCCTTATCAGCCACAAGATTCAATTTAGCCCTACTTTGGTGGGAGATTACAAACTTCTAAACCTTACTTGGTTTGAGGCCCCTGCACCTTCGCAAGCACCTCTAGTGGCTTCTTCACCTCATAAAGCACCATCACAAGGATCCTCAGCAACTACGTCAGTAAGATCTCCAGGGAAAAAGAGGCATCCCAATCTTATTCTTATCTTTTCTATAGCCGCTGGTGTGCTTATACTTGCCATAATCACTGTACTTGTTATTTGTTCCCGCGCACTCCGAGAAGAGAAAGCTCCAGATCCTCACAAAGAAGCTGTAAAACCAAGGAACCTGGACGCTGGTTCATTTGGGGGATCTCTTCCTCACCCAGCAAGTACACGGTTTCTGTCATATGAAGAACTCAAAGAGGCAACTAGCAATTTTGAATCTGCTAGCATTCTAGGAGAAGGTGGGTTTGGCAAGGTTTACAGAGGCATCTTAGCCGATGGTACTGCTGTAGCGATTAAGAAGCTCACAAGTGGTGGGCCACAAGGTGATAAAGAATTCCAGGTGGAGATTGATATGCTTAGCCGTCTTCATCATCGTAATCTTGTGAAACTTGTGGGTTACTATAGTAGTCGAGATTCTTCTCAGCACCTACTTTGTTATGAGCTTGTTCCAAATGGCAGCCTCGAGGCTTGGCTCCATGGGCCTCTCGGGTTGAACTGTCCTCTTGATTGGGACACCAGAATGAAGATTGCACTTGATGCTGCAAGAGGACTTGCATACCTTCATGAAGACTCGCAACCCTCCGTTATACACAGAGATTTTAAAGCCTCTAATATACTCCTTGAAAACAACTTCAACGCCAAAGTTGCAGATTTTGGCCTAGCCAAACAAGCTCCTGAAGGCAGGGGTAATCACTTATCTACTCGTGTTATGGGCACATTTGGATATGTTGCGCCTGAATATGCAATGACGGGACACCTACTCGTCAAGAGTGATGTTTATAGTTACGGTGTGGTCCTTCTCGAATTGTTAACTGGTAGAAAACCTGTGGATATGTCACAACCTTCAGGCCAAGAAAATCTCGTCACTTGGACAAGGCCAGTTTTAAGAGACAAAGACCGGTTAGAAGAACTAGTCGATTCAAGACTTGAAGGAAAATACCCGAAAGAAGATTTCATAAGAGTATGCACAATCGCTGCAGCTTGTGTTGCACCTGAAGCTAGCCAGAGACCAACGATGGGCGAAGTGGTTCAGTCACTTAAAATGGTTCAACGGGTGGTTGAGTATCAAGACCCGGTTTTAAACACTTCAAATAAAGCTCGTCCTAACCGGAGACAATCATCAGCTACGTTCGAGTCAGAAGTAACCTCTTCTATGTTCTCTTCTGGTCCTTATTCTGGTCTAAGCGCTTTTGATCATGAAAATATTACACGAACAACTGTTTTCTCAGAAGATCTTCACGAAGGCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

725

Amino Acids

79.05

Weight (kDa)

6.91

Isoelectric Point (pI)

50.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	164 - 266	1e-06	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	381 - 653	1.6e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	384 - 655	3.9e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009544)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02010
malus_domestica	MD09G1283200.v1.1 MD17G1274000.v1.1
prunus_persica	Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1
pyrus_communis	pycom09g19120 pycom17g27200
rosa_chinensis	RchiOBHm_Chr3g0487601
rosa_laevigata	RLG00000022997
rosa_multiflora	Rmu_sc0005285.1_g000001 Rmu_sc0012412.1_g000008
rosa_roxburghii	Rroxscaffold_6G00394660
rosa_rugosa	Rorug03G0234600 Rorug03G0234700 Rorug03G0234800 Rorug03G0234900
rosa_samantha	Rh3AG283200 Rh3BG318900 Rh3CG317000 Rh3DG315200
rosa_wichuraiana	Rw3G025040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1388
AccII	CGCG	3 cut(s)	20, 211, 990
AciI	CCGC	6 cut(s)	68, 209, 211, 711, 939, 988
AclI	AACGTT	1 cut(s)	152
AclWI	GGATC	4 cut(s)	858, 871, 1010, 1078
AcsI	RAATTY	3 cut(s)	203, 575, 1259
AcuI	CTGAAG	3 cut(s)	1623, 1755, 1941
AdeI	CACNNNGTG	2 cut(s)	502, 1250
AfaI	GTAC	3 cut(s)	972, 1093, 1207
AfeI	AGCGCT	1 cut(s)	2113
AfiI	CCNNNNNNNGG	8 cut(s)	55, 336, 359, 680, 760, 860, 1943, 2102
AflIII	ACRYGT	1 cut(s)	561
AgeI	ACCGGT	1 cut(s)	1824
AhlI	ACTAGT	1 cut(s)	1837
AjnI	CCWGG	4 cut(s)	352, 892, 1047, 1263
AjuI	GAANNNNNNNTTGG	2 cut(s)	1155, 1187
Alw21I	GWGCWC	1 cut(s)	26
Alw26I	GTCTC	5 cut(s)	354, 599, 1809, 1927, 2032
AlwI	GGATC	4 cut(s)	858, 871, 1010, 1078
Ama87I	CYCGRG	2 cut(s)	1388, 1412
Aor51HI	AGCGCT	1 cut(s)	2113
AoxI	GGCC	7 cut(s)	801, 1241, 1406, 1582, 1774, 1802, 2170
ApeKI	GCWGC	4 cut(s)	1383, 1466, 1901, 1904
ApoI	RAATTY	3 cut(s)	203, 575, 1259
AsiGI	ACCGGT	1 cut(s)	1824
Asp700I	GAANNNNTTC	3 cut(s)	563, 1953, 2160
AspLEI	GCGC	3 cut(s)	992, 1660, 2114
AspS9I	GGNCC	5 cut(s)	802, 1241, 1406, 1720, 2093
AsuC2I	CCSGG	1 cut(s)	2000
AsuHPI	GGTGA	8 cut(s)	138, 150, 162, 596, 661, 831, 1073, 1262
AsuNHI	GCTAGC	2 cut(s)	1148, 1925
AvaI	CYCGRG	2 cut(s)	1388, 1412
AvaII	GGWCC	2 cut(s)	1720, 2093
BaeGI	GKGCMC	1 cut(s)	1643
BaeI	ACNNNNGTAYC	2 cut(s)	1197, 1230
BamHI	GGATCC	1 cut(s)	863
BanII	GRGCYC	1 cut(s)	26
BauI	CACGAG	1 cut(s)	1629
BbsI	GAAGAC	2 cut(s)	1283, 1500
Bbv12I	GWGCWC	1 cut(s)	26
BbvCI	CCTCAGC	2 cut(s)	255, 868
BbvI	GCAGC	4 cut(s)	1395, 1453, 1888, 1916
BccI	CCATC	4 cut(s)	655, 862, 1196, 1936
BceAI	ACGGC	1 cut(s)	1272
BcgI	CGANNNNNNTGC	2 cut(s)	1190, 1224
BciT130I	CCWGG	4 cut(s)	354, 894, 1049, 1265
BciVI	GTATCC	1 cut(s)	516
BclI	TGATCA	2 cut(s)	292, 2119
BcnI	CCSGG	1 cut(s)	2000
BcoDI	GTCTC	5 cut(s)	354, 599, 1809, 1927, 2032
BcuI	ACTAGT	1 cut(s)	1837
BfmI	CTRYAG	4 cut(s)	933, 1212, 1327, 1902
BfoI	RGCGCY	1 cut(s)	2115
BfuI	GTATCC	1 cut(s)	516
BglI	GCCNNNNNGGC	1 cut(s)	365
BglII	AGATCT	2 cut(s)	887, 2158
BisI	GCNGC	5 cut(s)	939, 1384, 1467, 1902, 1905
BlpI	GCTNAGC	2 cut(s)	635, 1283
BlsI	GCNGC	5 cut(s)	940, 1385, 1468, 1903, 1906
Bme1390I	CCNGG	5 cut(s)	354, 894, 1049, 1265, 2000
Bme18I	GGWCC	2 cut(s)	1720, 2093
BmeT110I	CYCGRG	2 cut(s)	1388, 1412
BmgT120I	GGNCC	5 cut(s)	802, 1241, 1406, 1720, 2093
BmiI	GGNNCC	5 cut(s)	358, 804, 865, 1046, 1400
BmrFI	CCNGG	5 cut(s)	354, 894, 1049, 1265, 2000
BmsI	GCATC	6 cut(s)	109, 400, 716, 916, 1200, 1453
BmtI	GCTAGC	2 cut(s)	1152, 1929
BpiI	GAAGAC	2 cut(s)	1283, 1500
BpmI	CTGGAG	4 cut(s)	363, 375, 876, 996
Bpu10I	CCTNAGC	2 cut(s)	255, 868
Bpu1102I	GCTNAGC	2 cut(s)	635, 1283
BpuMI	CCSGG	1 cut(s)	2000
Bsa29I	ATCGAT	1 cut(s)	659
BsaBI	GATNNNNATC	1 cut(s)	665
BsaI	GGTCTC	2 cut(s)	599, 1927
BsaJI	CCNNGG	4 cut(s)	209, 893, 1040, 1402
BsaWI	WCCGGW	2 cut(s)	1824, 2034
Bsc4I	CCNNNNNNNGG	8 cut(s)	55, 336, 359, 680, 760, 860, 1943, 2102
Bse118I	RCCGGY	2 cut(s)	359, 1824
Bse1I	ACTGG	5 cut(s)	140, 173, 522, 1744, 1805
Bse3DI	GCAATG	2 cut(s)	400, 1677
Bse8I	GATNNNNATC	1 cut(s)	665
BseBI	CCWGG	4 cut(s)	354, 894, 1049, 1265
BseCI	ATCGAT	1 cut(s)	659
BseDI	CCNNGG	4 cut(s)	209, 893, 1040, 1402
BseGI	GGATG	5 cut(s)	295, 522, 654, 707, 907
BseJI	GATNNNNATC	1 cut(s)	665
BseLI	CCNNNNNNNGG	8 cut(s)	55, 336, 359, 680, 760, 860, 1943, 2102
BseMI	GCAATG	2 cut(s)	400, 1677
BseMII	CTCAG	7 cut(s)	269, 359, 434, 708, 882, 1361, 2166
BseNI	ACTGG	5 cut(s)	140, 173, 522, 1744, 1805
BseSI	GKGCMC	1 cut(s)	1643
BseXI	GCAGC	4 cut(s)	1395, 1453, 1888, 1916
BseYI	CCCAGC	2 cut(s)	585, 1084
BsgI	GTGCAG	1 cut(s)	792
Bsh1236I	CGCG	3 cut(s)	20, 211, 990
BshFI	GGCC	7 cut(s)	803, 1243, 1408, 1584, 1776, 1804, 2172
BshTI	ACCGGT	1 cut(s)	1824
BshVI	ATCGAT	1 cut(s)	659
BsiHKAI	GWGCWC	1 cut(s)	26
BsiHKCI	CYCGRG	2 cut(s)	1388, 1412
BsiSI	CCGG	4 cut(s)	360, 1825, 2000, 2035
BslFI	GGGAC	2 cut(s)	1451, 1692
BslI	CCNNNNNNNGG	8 cut(s)	55, 336, 359, 680, 760, 860, 1943, 2102
BsmAI	GTCTC	5 cut(s)	354, 599, 1809, 1927, 2032
BsmFI	GGGAC	2 cut(s)	1451, 1692
BsmI	GAATGC	3 cut(s)	259, 346, 1152
BsnI	GGCC	7 cut(s)	803, 1243, 1408, 1584, 1776, 1804, 2172
Bso31I	GGTCTC	2 cut(s)	599, 1927
BsoBI	CYCGRG	2 cut(s)	1388, 1412
Bsp1286I	GDGCHC	2 cut(s)	26, 1643
Bsp143I	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
Bsp1720I	GCTNAGC	2 cut(s)	635, 1283
Bsp19I	CCATGG	1 cut(s)	1402
Bsp68I	TCGCGA	1 cut(s)	20
BspACI	CCGC	6 cut(s)	68, 209, 211, 711, 939, 988
BspANI	GGCC	7 cut(s)	803, 1243, 1408, 1584, 1776, 1804, 2172
BspCNI	CTCAG	7 cut(s)	268, 358, 433, 707, 881, 1360, 2165
BspDI	ATCGAT	1 cut(s)	659
BspFNI	CGCG	3 cut(s)	20, 211, 990
BspHI	TCATGA	2 cut(s)	1489, 2122
BspLI	GGNNCC	5 cut(s)	358, 804, 865, 1046, 1400
BspMAI	CTGCAG	1 cut(s)	1906
BspOI	GCTAGC	2 cut(s)	1152, 1929
BspPI	GGATC	4 cut(s)	858, 871, 1010, 1078
BspQI	GCTCTTC	1 cut(s)	31
BspTNI	GGTCTC	2 cut(s)	599, 1927
BsrDI	GCAATG	2 cut(s)	400, 1677
BsrFI	RCCGGY	2 cut(s)	359, 1824
BsrI	ACTGG	5 cut(s)	140, 173, 522, 1744, 1805
BssAI	RCCGGY	2 cut(s)	359, 1824
BssECI	CCNNGG	4 cut(s)	209, 893, 1040, 1402
BssMI	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
BssSI	CACGAG	1 cut(s)	1629
BssT1I	CCWWGG	2 cut(s)	1040, 1402
Bst2BI	CACGAG	1 cut(s)	1629
Bst2UI	CCWGG	4 cut(s)	354, 894, 1049, 1265
Bst4CI	ACNGT	5 cut(s)	970, 1098, 1424, 1715, 2146
Bst6I	CTCTTC	6 cut(s)	31, 727, 996, 1080, 2080, 2092
BstAPI	GCANNNNNTGC	1 cut(s)	1463
BstC8I	GCNNGC	5 cut(s)	22, 361, 819, 1150, 1927
BstDSI	CCRYGG	2 cut(s)	209, 1402
BstF5I	GGATG	5 cut(s)	295, 522, 654, 707, 907
BstFNI	CGCG	3 cut(s)	20, 211, 990
BstH2I	RGCGCY	1 cut(s)	2115
BstHHI	GCGC	3 cut(s)	992, 1660, 2114
BstKTI	GATC	8 cut(s)	187, 295, 866, 890, 1018, 1073, 2122, 2161
BstMAI	GTCTC	5 cut(s)	354, 599, 1809, 1927, 2032
BstMBI	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
BstNI	CCWGG	4 cut(s)	354, 894, 1049, 1265
BstNSI	RCATGY	1 cut(s)	565
BstSCI	CCNGG	5 cut(s)	352, 892, 1047, 1263, 1998
BstSFI	CTRYAG	4 cut(s)	933, 1212, 1327, 1902
BstSLI	GKGCMC	1 cut(s)	1643
BstUI	CGCG	3 cut(s)	20, 211, 990
BstV1I	GCAGC	4 cut(s)	1395, 1453, 1888, 1916
BstV2I	GAAGAC	2 cut(s)	1283, 1500
BstX2I	RGATCY	5 cut(s)	863, 887, 1015, 1070, 2158
BstYI	RGATCY	5 cut(s)	863, 887, 1015, 1070, 2158
Bsu15I	ATCGAT	1 cut(s)	659
BsuI	GTATCC	1 cut(s)	516
BsuRI	GGCC	7 cut(s)	803, 1243, 1408, 1584, 1776, 1804, 2172
BsuTUI	ATCGAT	1 cut(s)	659
BtgI	CCRYGG	2 cut(s)	209, 1402
BtsCI	GGATG	5 cut(s)	295, 522, 654, 707, 907
BtsI	GCAGTG	1 cut(s)	496
BtsIMutI	CAGTG	4 cut(s)	109, 180, 496, 966
BtuMI	TCGCGA	1 cut(s)	20
Cac8I	GCNNGC	5 cut(s)	22, 361, 819, 1150, 1927
CciI	TCATGA	2 cut(s)	1489, 2122
CfoI	GCGC	3 cut(s)	992, 1660, 2114
Cfr10I	RCCGGY	2 cut(s)	359, 1824
Cfr13I	GGNCC	5 cut(s)	802, 1241, 1406, 1720, 2093
Cfr42I	CCGCGG	1 cut(s)	212
ClaI	ATCGAT	1 cut(s)	659
CseI	GACGC	1 cut(s)	1061
Csp6I	GTAC	3 cut(s)	971, 1092, 1206
CspAI	ACCGGT	1 cut(s)	1824
CviAII	CATG	6 cut(s)	128, 331, 562, 1403, 1490, 2123
CviQI	GTAC	3 cut(s)	971, 1092, 1206
DpnI	GATC	8 cut(s)	186, 294, 865, 889, 1017, 1072, 2121, 2160
DpnII	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
DraI	TTTAAA	2 cut(s)	1528, 2007
DraIII	CACNNNGTG	2 cut(s)	502, 1250
Eam1104I	CTCTTC	6 cut(s)	31, 727, 996, 1080, 2080, 2092
EarI	CTCTTC	6 cut(s)	31, 727, 996, 1080, 2080, 2092
Ecl136II	GAGCTC	1 cut(s)	24
Eco130I	CCWWGG	2 cut(s)	1040, 1402
Eco24I	GRGCYC	1 cut(s)	26
Eco31I	GGTCTC	2 cut(s)	599, 1927
Eco32I	GATATC	1 cut(s)	667
Eco47I	GGWCC	2 cut(s)	1720, 2093
Eco47III	AGCGCT	1 cut(s)	2113
Eco53kI	GAGCTC	1 cut(s)	24
Eco57I	CTGAAG	3 cut(s)	1623, 1755, 1941
Eco88I	CYCGRG	2 cut(s)	1388, 1412
EcoICRI	GAGCTC	1 cut(s)	24
EcoO109I	RGGNCCY	1 cut(s)	802
EcoRI	GAATTC	1 cut(s)	1259
EcoRII	CCWGG	4 cut(s)	352, 892, 1047, 1263
EcoRV	GATATC	1 cut(s)	667
EcoT14I	CCWWGG	2 cut(s)	1040, 1402
EcoT38I	GRGCYC	1 cut(s)	26
ErhI	CCWWGG	2 cut(s)	1040, 1402
FaeI	CATG	6 cut(s)	131, 334, 565, 1406, 1493, 2126
FaqI	GGGAC	2 cut(s)	1451, 1692
FatI	CATG	6 cut(s)	127, 330, 561, 1402, 1489, 2122
FauI	CCCGC	1 cut(s)	995
FauNDI	CATATG	1 cut(s)	1108
FbaI	TGATCA	2 cut(s)	292, 2119
Fnu4HI	GCNGC	5 cut(s)	939, 1384, 1467, 1902, 1905
FokI	GGATG	5 cut(s)	282, 509, 661, 694, 894
FriOI	GRGCYC	1 cut(s)	26
Fsp4HI	GCNGC	5 cut(s)	939, 1384, 1467, 1902, 1905
GlaI	GCGC	3 cut(s)	991, 1659, 2113
GluI	GCNGC	5 cut(s)	939, 1384, 1467, 1902, 1905
GsaI	CCCAGC	2 cut(s)	589, 1088
GsuI	CTGGAG	4 cut(s)	363, 375, 876, 996
HaeII	RGCGCY	1 cut(s)	2115
HaeIII	GGCC	7 cut(s)	803, 1243, 1408, 1584, 1776, 1804, 2172
HapII	CCGG	4 cut(s)	360, 1825, 2000, 2035
HgaI	GACGC	1 cut(s)	1061
HhaI	GCGC	3 cut(s)	992, 1660, 2114
Hin1II	CATG	6 cut(s)	131, 334, 565, 1406, 1493, 2126
Hin6I	GCGC	3 cut(s)	990, 1658, 2112
HinP1I	GCGC	3 cut(s)	990, 1658, 2112
HincII	GTYRAC	1 cut(s)	1737
HindII	GTYRAC	1 cut(s)	1737
HindIII	AAGCTT	1 cut(s)	637
HinfI	GANTC	7 cut(s)	32, 742, 1142, 1340, 1496, 1844, 2060
HpaI	GTTAAC	1 cut(s)	1737
HpaII	CCGG	4 cut(s)	360, 1825, 2000, 2035
HphI	GGTGA	8 cut(s)	138, 150, 162, 596, 661, 831, 1073, 1262
Hpy166II	GTNNAC	3 cut(s)	1094, 1183, 1737
Hpy188I	TCNGA	7 cut(s)	31, 292, 463, 544, 998, 2065, 2155
Hpy8I	GTNNAC	3 cut(s)	1094, 1183, 1737
HpyCH4III	ACNGT	5 cut(s)	970, 1098, 1424, 1715, 2146
HpyCH4IV	ACGT	3 cut(s)	152, 878, 2054
HpySE526I	ACGT	3 cut(s)	152, 878, 2054
Hsp92II	CATG	6 cut(s)	131, 334, 565, 1406, 1493, 2126
HspAI	GCGC	3 cut(s)	990, 1658, 2112
KroI	GCCGGC	1 cut(s)	359
KroNI	GCCGGC	1 cut(s)	361
Ksp22I	TGATCA	2 cut(s)	292, 2119
KspAI	GTTAAC	1 cut(s)	1737
KspI	CCGCGG	1 cut(s)	212
Kzo9I	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
LguI	GCTCTTC	1 cut(s)	31
LmnI	GCTCC	5 cut(s)	356, 382, 1015, 1404, 1603
Lsp1109I	GCAGC	4 cut(s)	1395, 1453, 1888, 1916
LweI	GCATC	6 cut(s)	109, 400, 716, 916, 1200, 1453
MaeII	ACGT	3 cut(s)	152, 878, 2054
MaeIII	GTNAC	6 cut(s)	1322, 1709, 1761, 1789, 1959, 2068
MalI	GATC	8 cut(s)	186, 294, 865, 889, 1017, 1072, 2121, 2160
MboI	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
MflI	RGATCY	5 cut(s)	863, 887, 1015, 1070, 2158
MhlI	GDGCHC	2 cut(s)	26, 1643
MluCI	AATT	9 cut(s)	110, 203, 448, 575, 684, 746, 1135, 1259, 1730
MlyI	GAGTC	2 cut(s)	1490, 2069
MmeI	TCCRAC	1 cut(s)	536
MroNI	GCCGGC	1 cut(s)	359
MroXI	GAANNNNTTC	3 cut(s)	563, 1953, 2160
MseI	TTAA	9 cut(s)	416, 474, 621, 1221, 1527, 1736, 1811, 1965, 2006
MslI	CAYNNNNRTG	2 cut(s)	659, 1445
MspA1I	CMGCKG	3 cut(s)	200, 211, 941
MspI	CCGG	4 cut(s)	360, 1825, 2000, 2035
MspR9I	CCNGG	5 cut(s)	354, 894, 1049, 1265, 2000
Mva1269I	GAATGC	3 cut(s)	259, 346, 1152
MvaI	CCWGG	4 cut(s)	354, 894, 1049, 1265
MvnI	CGCG	3 cut(s)	20, 211, 990
NaeI	GCCGGC	1 cut(s)	361
NciI	CCSGG	1 cut(s)	2000
NcoI	CCATGG	1 cut(s)	1402
NdeI	CATATG	1 cut(s)	1108
NdeII	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
NgoMIV	GCCGGC	1 cut(s)	359
NheI	GCTAGC	2 cut(s)	1148, 1925
NlaIII	CATG	6 cut(s)	131, 334, 565, 1406, 1493, 2126
NlaIV	GGNNCC	5 cut(s)	358, 804, 865, 1046, 1400
NmuCI	GTSAC	3 cut(s)	1761, 1789, 1959
NruI	TCGCGA	1 cut(s)	20
NspI	RCATGY	1 cut(s)	565
PaeR7I	CTCGAG	1 cut(s)	1388
PagI	TCATGA	2 cut(s)	1489, 2122
PciI	ACATGT	1 cut(s)	561
PciSI	GCTCTTC	1 cut(s)	31
PctI	GAATGC	3 cut(s)	259, 346, 1152
PdiI	GCCGGC	1 cut(s)	361
PdmI	GAANNNNTTC	3 cut(s)	563, 1953, 2160
PfeI	GAWTC	5 cut(s)	32, 742, 1142, 1340, 1844
PfoI	TCCNGGA	1 cut(s)	352
PinAI	ACCGGT	1 cut(s)	1824
PkrI	GCNGC	5 cut(s)	940, 1385, 1468, 1903, 1906
PleI	GAGTC	2 cut(s)	1490, 2068
PpsI	GAGTC	2 cut(s)	1490, 2068
PscI	ACATGT	1 cut(s)	561
Psp124BI	GAGCTC	1 cut(s)	26
Psp1406I	AACGTT	1 cut(s)	152
Psp6I	CCWGG	4 cut(s)	352, 892, 1047, 1263
PspFI	CCCAGC	2 cut(s)	585, 1084
PspGI	CCWGG	4 cut(s)	352, 892, 1047, 1263
PspN4I	GGNNCC	5 cut(s)	358, 804, 865, 1046, 1400
PspPI	GGNCC	5 cut(s)	802, 1241, 1406, 1720, 2093
PspXI	VCTCGAGB	1 cut(s)	1388
PstI	CTGCAG	1 cut(s)	1906
PsuI	RGATCY	5 cut(s)	863, 887, 1015, 1070, 2158
PvuII	CAGCTG	1 cut(s)	200
RruI	TCGCGA	1 cut(s)	20
RsaI	GTAC	3 cut(s)	972, 1093, 1207
RsaNI	GTAC	3 cut(s)	971, 1092, 1206
RseI	CAYNNNNRTG	2 cut(s)	659, 1445
SacI	GAGCTC	1 cut(s)	26
SacII	CCGCGG	1 cut(s)	212
SapI	GCTCTTC	1 cut(s)	31
SaqAI	TTAA	9 cut(s)	416, 474, 621, 1221, 1527, 1736, 1811, 1965, 2006
SatI	GCNGC	5 cut(s)	939, 1384, 1467, 1902, 1905
Sau3AI	GATC	8 cut(s)	184, 292, 863, 887, 1015, 1070, 2119, 2158
Sau96I	GGNCC	5 cut(s)	802, 1241, 1406, 1720, 2093
SchI	GAGTC	2 cut(s)	1490, 2069
ScrFI	CCNGG	5 cut(s)	354, 894, 1049, 1265, 2000
SduI	GDGCHC	2 cut(s)	26, 1643
SfaNI	GCATC	6 cut(s)	109, 400, 716, 916, 1200, 1453
SfcI	CTRYAG	4 cut(s)	933, 1212, 1327, 1902
Sfr274I	CTCGAG	1 cut(s)	1388
Sfr303I	CCGCGG	1 cut(s)	212
SgrBI	CCGCGG	1 cut(s)	212
SinI	GGWCC	2 cut(s)	1720, 2093
SlaI	CTCGAG	1 cut(s)	1388
SmiMI	CAYNNNNRTG	2 cut(s)	659, 1445
SmlI	CTYRAG	1 cut(s)	1388
SmoI	CTYRAG	1 cut(s)	1388
SpeI	ACTAGT	1 cut(s)	1837
Sse9I	AATT	9 cut(s)	110, 203, 448, 575, 684, 746, 1135, 1259, 1730
SsiI	CCGC	6 cut(s)	68, 209, 211, 711, 939, 988
SspI	AATATT	1 cut(s)	2131
SstI	GAGCTC	1 cut(s)	26
StyD4I	CCNGG	5 cut(s)	352, 892, 1047, 1263, 1998
StyI	CCWWGG	2 cut(s)	1040, 1402
TaaI	ACNGT	5 cut(s)	970, 1098, 1424, 1715, 2146
TaiI	ACGT	3 cut(s)	155, 881, 2057
TaqI	TCGA	8 cut(s)	5, 612, 659, 1336, 1389, 1728, 1842, 2058
TasI	AATT	9 cut(s)	110, 203, 448, 575, 684, 746, 1135, 1259, 1730
TatI	WGTACW	2 cut(s)	970, 1091
TauI	GCSGC	1 cut(s)	941
TfiI	GAWTC	5 cut(s)	32, 742, 1142, 1340, 1844
Tru1I	TTAA	9 cut(s)	416, 474, 621, 1221, 1527, 1736, 1811, 1965, 2006
Tru9I	TTAA	9 cut(s)	416, 474, 621, 1221, 1527, 1736, 1811, 1965, 2006
TscAI	CASTG	4 cut(s)	109, 187, 503, 973
TseFI	GTSAC	3 cut(s)	1761, 1789, 1959
TseI	GCWGC	4 cut(s)	1383, 1466, 1901, 1904
Tsp45I	GTSAC	3 cut(s)	1761, 1789, 1959
TspGWI	ACGGA	1 cut(s)	1498
TspRI	CASTG	4 cut(s)	109, 187, 503, 973
VpaK11BI	GGWCC	2 cut(s)	1720, 2093
XapI	RAATTY	3 cut(s)	203, 575, 1259
XceI	RCATGY	1 cut(s)	565
XhoI	CTCGAG	1 cut(s)	1388
XmnI	GAANNNNTTC	3 cut(s)	563, 1953, 2160

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.