Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009544

Physical Location & Seq

Genomic Coordinates Reverse (-)

36101889 .. 36107673

Gene Structure 5785 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1283200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGTCTGAGCTCACAGGAATATTGAACAAGGTTCAATGGTTGGTGATGAATCAGTTGCTTTTGTTCTGTTATGTGATATCCGTCACTGAAGCCAGTGAACCCTATGGTTTACTCAAAGCACCATCAGTATCTCTATCGAGTGCACCTTTGGCTGCACCAGCTATGCCAAATCTACCCCTGCCAGCCACTTTGCCAGTGTCCCACTACCATAGACGCTGGCAAAAACATTTCTTGCCACATGCCGCGCCAGTTGCTCTATCTCCTGCACACCCTCCTTTTTATGGACCCTTGATAACTGCTGGTCACCCCCCTACAGCTTCTCGATTGTCAAAGCCATCAATGAAGAGGAGTGGCTTGGCACCTCCACTTGCTAGCTTTAAGAATATCGCCCCAACACAGTCGAGTGCTGGTACAATTCCTTCTGGTTTAGCACAGCCCCCGCTCACTCCTTCCATTTCCAACTGTTGCAAGCCAGACATGGTACTGAGACGGGGAAGTCAGGGTTGCCACTGTGCTTATCCCATAAAGCTTGATATTCTCCTCCTAAATGTTTCACAAAATCCTAACTGGAATATTTTTCTTGAAGAACTAGCTTCCGAGCTTCATTTGCAAGTTTCTCAAATAGAGCTGATTAACTTTTATGTACTCGGATTATCAAGATTAAATATTTCGATGGATATTACTCCCAATTCAGGATCCAGTTTCTCCGCGAGTGATGCGTCCACAATAAACTCTTCTCTTGTCATGCACAAGGTTAAATTGAACCCTGCATTAGTGGGCGATTACAAACTCCTCAATATAACTTGGTTTAAGCCTCCACCTCCTTCTCATGCTCCTATTGCAGCATCACCAGTGGAAGGCCAAGCAAATCCACCCCCTACTCTTACATCCTTAAGTGCTTCAGATAAAGGGAGGCATTCAAATTGGAGTCTTATTATTGGTATTGGCATCGGCATATTGTTCATTGCCATTATATCTGTGCTCATACTTTGTTTGTGCACATTCCGTCAAGAGAAGACTAAAGCATCTCTTATAGAAACAGCCCCTGAAAAGCAGAGGACTGTTGATACAGTGCAAGCAGTAGGATCTTTACCTCACCCAAGCAGCACTCGATTTCTGGCATATGAAGAACTTAAACAAGCAACAAACAACTTTGAAAGCATACTCGGAGAGGGTGGTTTTGGCAGGGTGTTCAAGGGTGTCTTAAGTGATGGTACAGCTGTAGCAATTAAAAGACTTACCAATGGAGGGCAACAGGGGGATAAGGAGTTCTTGGTTGAGGTTGAAATGCTGAGCAGGCTGCATCATCGTAATCTGGTGAAACTTGTGGGGTACTATAGCAGTCGTGACTCTTCACAAAACTTACTTTGCTATGAGCTTGTACCAAATGGAAGCTTGGAGGCCTGGCTCCATGGTCCCCTGGGGGTAAATTGTCCTTTGGATTGGGACACCCGAATGAAAATTGCACTTGATGCTGCCAGAGGACTTGCTTACCTGCATGAAGACTCACAACCTTGTGTCATCCACAGAGATTTTAAAGCATCCAATATATTGCTTGAGAATAACTTTCATGCTAAAGTTGCTGATTTTGGCCTGGCCAAACAGGCACCTGAAGGCAGAGCAAATTATCTTTCTACCCGTGTGATGGGAACATTTGGGTATGTGGCTCCAGAGTACGCCATGACTGGACACCTACTTGTTAAAAGTGATGTTTACAGTTACGGAGTTGTCCTTCTCGAGTTACTCACTGGAAGAAAGCCTGTAGATATGTCACAGCCAGCCGGACAGGAGAATCTAGTCACTTGGTCGAGGCCAATTCTTAGAGACAAGGATAGACTGGATGAGCTCGCTGACCCTAGTCTTGAAGGAAAGTACCCAACGGAGGATTTTGTACGAGTTTGCACAATTGCAGCCGCTTGTGTTGCTCCTGAGGCAAGCCAACGCCCTACAATGGGTGAAGTGGTACAATCACTGAAGATGGTGCAACGAATCACAGAATATCAGGATACCATGTTAACGTCTTCCAATGCCAGACCCAATAACAGGGAGTCTTCAATAACCTTTGAATCCGATGTGTCATCTTCGATGTTCTCTTCTGGTCCTTACTCTGGTCTAAGTGCCTTTGAGAATGACAACGTCTCTCGAGCAGCTGTTTTCTCTGAAGATCTTCACGAAGGACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

728

Amino Acids

79.17

Weight (kDa)

6.86

Isoelectric Point (pI)

49.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	167 - 269	7.6e-08	Receptor-like PK ALE2 N-terminal domain
PK_Tyr_Ser-Thr	PF07714	385 - 658	5.8e-45	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	386 - 656	1.9e-43	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009544)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02010
malus_domestica	MD09G1283200.v1.1 MD17G1274000.v1.1
prunus_persica	Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1
pyrus_communis	pycom09g19120 pycom17g27200
rosa_chinensis	RchiOBHm_Chr3g0487601
rosa_laevigata	RLG00000022997
rosa_multiflora	Rmu_sc0005285.1_g000001 Rmu_sc0012412.1_g000008
rosa_roxburghii	Rroxscaffold_6G00394660
rosa_rugosa	Rorug03G0234600 Rorug03G0234700 Rorug03G0234800 Rorug03G0234900
rosa_samantha	Rh3AG283200 Rh3BG318900 Rh3CG317000 Rh3DG315200
rosa_wichuraiana	Rw3G025040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	495
Acc36I	ACCTGC	1 cut(s)	1503
AccB1I	GGYRCC	2 cut(s)	358, 1606
AccBSI	CCGCTC	1 cut(s)	442
AccII	CGCG	2 cut(s)	245, 710
AciI	CCGC	4 cut(s)	243, 440, 708, 1914
AclWI	GGATC	3 cut(s)	690, 703, 1093
AcoI	YGGCCR	1 cut(s)	1596
AcuI	CTGAAG	5 cut(s)	108, 885, 1632, 1994, 2181
AflII	CTTAAG	2 cut(s)	892, 1204
AjnI	CCWGG	3 cut(s)	1403, 1419, 1593
AloI	GAACNNNNNNTCC	2 cut(s)	1253, 1285
Alw21I	GWGCWC	5 cut(s)	12, 145, 984, 1001, 1848
Alw26I	GTCTC	3 cut(s)	481, 1818, 2143
Alw44I	GTGCAC	2 cut(s)	141, 997
AlwI	GGATC	3 cut(s)	690, 703, 1093
AlwNI	CAGNNNCTG	1 cut(s)	1046
Ama87I	CYCGRG	2 cut(s)	1736, 2142
AoxI	GGCC	5 cut(s)	859, 1401, 1591, 1596, 1811
ApaLI	GTGCAC	2 cut(s)	141, 997
ApeKI	GCWGC	7 cut(s)	152, 842, 1104, 1300, 1475, 1910, 2147
Asp700I	GAANNNNTTC	1 cut(s)	2166
AspLEI	GCGC	1 cut(s)	247
AspS9I	GGNCC	3 cut(s)	284, 1415, 2099
AsuHPI	GGTGA	6 cut(s)	55, 296, 840, 1088, 1330, 1967
AsuNHI	GCTAGC	1 cut(s)	371
AvaI	CYCGRG	2 cut(s)	1736, 2142
AvaII	GGWCC	3 cut(s)	284, 1415, 2099
AxyI	CCTNAGG	1 cut(s)	1929
BaeGI	GKGCMC	2 cut(s)	145, 1001
BaeI	ACNNNNGTAYC	2 cut(s)	1206, 1239
BalI	TGGCCA	1 cut(s)	1598
BamHI	GGATCC	1 cut(s)	695
BanI	GGYRCC	2 cut(s)	358, 1606
BanII	GRGCYC	2 cut(s)	12, 1848
BarI	GAAGNNNNNNTAC	2 cut(s)	403, 435
BbsI	GAAGAC	4 cut(s)	1022, 1509, 2013, 2043
Bbv12I	GWGCWC	5 cut(s)	12, 145, 984, 1001, 1848
BbvI	GCAGC	7 cut(s)	139, 854, 1116, 1287, 1462, 1922, 2159
BccI	CCATC	6 cut(s)	130, 343, 667, 1205, 1639, 1972
BciT130I	CCWGG	3 cut(s)	1405, 1421, 1595
BciVI	GTATCC	1 cut(s)	1999
BcoDI	GTCTC	3 cut(s)	481, 1818, 2143
BfaI	CTAG	4 cut(s)	372, 590, 1796, 1857
BfmI	CTRYAG	4 cut(s)	312, 1221, 1336, 1761
BfrI	CTTAAG	2 cut(s)	892, 1204
BfuAI	ACCTGC	1 cut(s)	1503
BfuI	GTATCC	1 cut(s)	1999
BglI	GCCNNNNNGGC	1 cut(s)	1604
BglII	AGATCT	1 cut(s)	2164
BisI	GCNGC	9 cut(s)	153, 243, 843, 1105, 1301, 1476, 1911, 1914, 2148
BlpI	GCTNAGC	1 cut(s)	1292
BlsI	GCNGC	9 cut(s)	154, 244, 844, 1106, 1302, 1477, 1912, 1915, 2149
Bme1390I	CCNGG	3 cut(s)	1405, 1421, 1595
Bme18I	GGWCC	3 cut(s)	284, 1415, 2099
BmeT110I	CYCGRG	2 cut(s)	1736, 2142
BmgT120I	GGNCC	3 cut(s)	284, 1415, 2099
BmiI	GGNNCC	7 cut(s)	286, 360, 697, 1409, 1417, 1608, 1668
BmrFI	CCNGG	3 cut(s)	1405, 1421, 1595
BmsI	GCATC	7 cut(s)	706, 854, 957, 1034, 1312, 1462, 1550
BmtI	GCTAGC	1 cut(s)	375
BoxI	GACNNNNGTC	1 cut(s)	1857
BpiI	GAAGAC	4 cut(s)	1022, 1509, 2013, 2043
BpmI	CTGGAG	1 cut(s)	1653
Bpu1102I	GCTNAGC	1 cut(s)	1292
BpuEI	CTTGAG	1 cut(s)	1577
BsaJI	CCNNGG	3 cut(s)	1411, 1419, 1420
Bse1I	ACTGG	9 cut(s)	93, 194, 248, 572, 699, 851, 1690, 1753, 1842
Bse21I	CCTNAGG	1 cut(s)	1929
Bse3DI	GCAATG	1 cut(s)	963
BseBI	CCWGG	3 cut(s)	1405, 1421, 1595
BseDI	CCNNGG	3 cut(s)	1411, 1419, 1420
BseGI	GGATG	4 cut(s)	887, 1521, 1541, 1846
BseMI	GCAATG	1 cut(s)	963
BseMII	CTCAG	3 cut(s)	476, 1283, 1920
BseNI	ACTGG	9 cut(s)	93, 194, 248, 572, 699, 851, 1690, 1753, 1842
BseRI	GAGGAG	3 cut(s)	361, 530, 782
BseSI	GKGCMC	2 cut(s)	145, 1001
BseXI	GCAGC	7 cut(s)	139, 854, 1116, 1287, 1462, 1922, 2159
BsgI	GTGCAG	2 cut(s)	138, 249
Bsh1236I	CGCG	2 cut(s)	245, 710
BshFI	GGCC	5 cut(s)	861, 1403, 1593, 1598, 1813
BshNI	GGYRCC	2 cut(s)	358, 1606
BsiHKAI	GWGCWC	5 cut(s)	12, 145, 984, 1001, 1848
BsiHKCI	CYCGRG	2 cut(s)	1736, 2142
BsiSI	CCGG	1 cut(s)	1782
BslFI	GGGAC	3 cut(s)	184, 1401, 1460
BsmAI	GTCTC	3 cut(s)	481, 1818, 2143
BsmBI	CGTCTC	2 cut(s)	481, 2143
BsmFI	GGGAC	3 cut(s)	184, 1401, 1460
BsmI	GAATGC	1 cut(s)	916
BsnI	GGCC	5 cut(s)	861, 1403, 1593, 1598, 1813
BsoBI	CYCGRG	2 cut(s)	1736, 2142
Bsp1286I	GDGCHC	5 cut(s)	12, 145, 984, 1001, 1848
Bsp143I	GATC	3 cut(s)	695, 1085, 2164
Bsp1720I	GCTNAGC	1 cut(s)	1292
Bsp19I	CCATGG	1 cut(s)	1411
BspACI	CCGC	4 cut(s)	243, 440, 708, 1914
BspANI	GGCC	5 cut(s)	861, 1403, 1593, 1598, 1813
BspCNI	CTCAG	3 cut(s)	477, 1284, 1921
BspFNI	CGCG	2 cut(s)	245, 710
BspLI	GGNNCC	7 cut(s)	286, 360, 697, 1409, 1417, 1608, 1668
BspMI	ACCTGC	1 cut(s)	1503
BspOI	GCTAGC	1 cut(s)	375
BspPI	GGATC	3 cut(s)	690, 703, 1093
BspT107I	GGYRCC	2 cut(s)	358, 1606
BspTI	CTTAAG	2 cut(s)	892, 1204
BsrBI	CCGCTC	1 cut(s)	442
BsrDI	GCAATG	1 cut(s)	963
BsrI	ACTGG	9 cut(s)	93, 194, 248, 572, 699, 851, 1690, 1753, 1842
BssECI	CCNNGG	3 cut(s)	1411, 1419, 1420
BssMI	GATC	3 cut(s)	695, 1085, 2164
BssT1I	CCWWGG	1 cut(s)	1411
Bst2UI	CCWGG	3 cut(s)	1405, 1421, 1595
Bst4CI	ACNGT	6 cut(s)	399, 464, 512, 1063, 1072, 1718
Bst6I	CTCTTC	4 cut(s)	338, 739, 1357, 2098
BstAFI	CTTAAG	2 cut(s)	892, 1204
BstAPI	GCANNNNNTGC	1 cut(s)	1472
BstC8I	GCNNGC	9 cut(s)	183, 218, 373, 470, 1077, 1298, 1779, 1848, 1936
BstDEI	CTNAG	6 cut(s)	6, 485, 1292, 1820, 1929, 2114
BstDSI	CCRYGG	1 cut(s)	1411
BstEII	GGTNACC	1 cut(s)	302
BstF5I	GGATG	4 cut(s)	887, 1521, 1541, 1846
BstFNI	CGCG	2 cut(s)	245, 710
BstHHI	GCGC	1 cut(s)	247
BstKTI	GATC	3 cut(s)	698, 1088, 2167
BstMAI	GTCTC	3 cut(s)	481, 1818, 2143
BstMBI	GATC	3 cut(s)	695, 1085, 2164
BstNI	CCWGG	3 cut(s)	1405, 1421, 1595
BstNSI	RCATGY	1 cut(s)	242
BstPAI	GACNNNNGTC	1 cut(s)	1857
BstPI	GGTNACC	1 cut(s)	302
BstSCI	CCNGG	3 cut(s)	1403, 1419, 1593
BstSFI	CTRYAG	4 cut(s)	312, 1221, 1336, 1761
BstSLI	GKGCMC	2 cut(s)	145, 1001
BstUI	CGCG	2 cut(s)	245, 710
BstV1I	GCAGC	7 cut(s)	139, 854, 1116, 1287, 1462, 1922, 2159
BstV2I	GAAGAC	4 cut(s)	1022, 1509, 2013, 2043
BstX2I	RGATCY	3 cut(s)	695, 1085, 2164
BstYI	RGATCY	3 cut(s)	695, 1085, 2164
Bsu36I	CCTNAGG	1 cut(s)	1929
BsuI	GTATCC	1 cut(s)	1999
BsuRI	GGCC	5 cut(s)	861, 1403, 1593, 1598, 1813
BtgI	CCRYGG	1 cut(s)	1411
BtsCI	GGATG	4 cut(s)	887, 1521, 1541, 1846
BtsIMutI	CAGTG	8 cut(s)	84, 100, 201, 508, 858, 1077, 1746, 1970
BveI	ACCTGC	1 cut(s)	1503
Cac8I	GCNNGC	9 cut(s)	183, 218, 373, 470, 1077, 1298, 1779, 1848, 1936
CaiI	CAGNNNCTG	1 cut(s)	1046
CfoI	GCGC	1 cut(s)	247
Cfr13I	GGNCC	3 cut(s)	284, 1415, 2099
CseI	GACGC	2 cut(s)	222, 708
CviAII	CATG	9 cut(s)	239, 478, 745, 830, 1412, 1499, 1571, 1681, 2011
DdeI	CTNAG	6 cut(s)	6, 485, 1292, 1820, 1929, 2114
DpnI	GATC	3 cut(s)	697, 1087, 2166
DpnII	GATC	3 cut(s)	695, 1085, 2164
DraI	TTTAAA	1 cut(s)	1537
DrdI	GACNNNNNNGTC	1 cut(s)	495
DseDI	GACNNNNNNGTC	1 cut(s)	495
EaeI	YGGCCR	1 cut(s)	1596
Eam1104I	CTCTTC	4 cut(s)	338, 739, 1357, 2098
EarI	CTCTTC	4 cut(s)	338, 739, 1357, 2098
Ecl136II	GAGCTC	2 cut(s)	10, 1846
Eco130I	CCWWGG	1 cut(s)	1411
Eco147I	AGGCCT	1 cut(s)	1403
Eco24I	GRGCYC	2 cut(s)	12, 1848
Eco32I	GATATC	1 cut(s)	78
Eco47I	GGWCC	3 cut(s)	284, 1415, 2099
Eco53kI	GAGCTC	2 cut(s)	10, 1846
Eco57I	CTGAAG	5 cut(s)	108, 885, 1632, 1994, 2181
Eco81I	CCTNAGG	1 cut(s)	1929
Eco88I	CYCGRG	2 cut(s)	1736, 2142
Eco91I	GGTNACC	1 cut(s)	302
EcoICRI	GAGCTC	2 cut(s)	10, 1846
EcoO65I	GGTNACC	1 cut(s)	302
EcoRII	CCWGG	3 cut(s)	1403, 1419, 1593
EcoRV	GATATC	1 cut(s)	78
EcoT14I	CCWWGG	1 cut(s)	1411
EcoT38I	GRGCYC	2 cut(s)	12, 1848
ErhI	CCWWGG	1 cut(s)	1411
Esp3I	CGTCTC	2 cut(s)	481, 2143
FaeI	CATG	9 cut(s)	242, 481, 748, 833, 1415, 1502, 1574, 1684, 2014
FalI	AAGNNNNNCTT	6 cut(s)	1014, 1046, 1187, 1219, 1256, 1288
FaqI	GGGAC	3 cut(s)	184, 1401, 1460
FatI	CATG	9 cut(s)	238, 477, 744, 829, 1411, 1498, 1570, 1680, 2010
FauI	CCCGC	1 cut(s)	447
FauNDI	CATATG	1 cut(s)	1123
Fnu4HI	GCNGC	9 cut(s)	153, 243, 843, 1105, 1301, 1476, 1911, 1914, 2148
FokI	GGATG	4 cut(s)	874, 1508, 1528, 1853
FriOI	GRGCYC	2 cut(s)	12, 1848
Fsp4HI	GCNGC	9 cut(s)	153, 243, 843, 1105, 1301, 1476, 1911, 1914, 2148
FspBI	CTAG	4 cut(s)	372, 590, 1796, 1857
GlaI	GCGC	1 cut(s)	246
GluI	GCNGC	9 cut(s)	153, 243, 843, 1105, 1301, 1476, 1911, 1914, 2148
GsuI	CTGGAG	1 cut(s)	1653
HaeIII	GGCC	5 cut(s)	861, 1403, 1593, 1598, 1813
HapII	CCGG	1 cut(s)	1782
HgaI	GACGC	2 cut(s)	222, 708
HhaI	GCGC	1 cut(s)	247
Hin1II	CATG	9 cut(s)	242, 481, 748, 833, 1415, 1502, 1574, 1684, 2014
Hin6I	GCGC	1 cut(s)	245
HinP1I	GCGC	1 cut(s)	245
HincII	GTYRAC	1 cut(s)	2016
HindII	GTYRAC	1 cut(s)	2016
HindIII	AAGCTT	2 cut(s)	527, 1393
HinfI	GANTC	8 cut(s)	49, 928, 1349, 1505, 1792, 1989, 2048, 2066
HpaI	GTTAAC	1 cut(s)	2016
HpaII	CCGG	1 cut(s)	1782
HphI	GGTGA	6 cut(s)	55, 296, 840, 1088, 1330, 1967
Hpy166II	GTNNAC	7 cut(s)	98, 110, 143, 723, 999, 1714, 2016
Hpy188I	TCNGA	7 cut(s)	7, 598, 650, 904, 1169, 2071, 2161
Hpy8I	GTNNAC	7 cut(s)	98, 110, 143, 723, 999, 1714, 2016
HpyAV	CCTTC	8 cut(s)	429, 459, 834, 851, 1607, 1742, 1859, 2168
HpyCH4III	ACNGT	6 cut(s)	399, 464, 512, 1063, 1072, 1718
HpyCH4IV	ACGT	2 cut(s)	2018, 2136
HpyF3I	CTNAG	6 cut(s)	6, 485, 1292, 1820, 1929, 2114
HpySE526I	ACGT	2 cut(s)	2018, 2136
Hsp92II	CATG	9 cut(s)	242, 481, 748, 833, 1415, 1502, 1574, 1684, 2014
HspAI	GCGC	1 cut(s)	245
KspAI	GTTAAC	1 cut(s)	2016
Kzo9I	GATC	3 cut(s)	695, 1085, 2164
LmnI	GCTCC	4 cut(s)	838, 1413, 1672, 1930
Lsp1109I	GCAGC	7 cut(s)	139, 854, 1116, 1287, 1462, 1922, 2159
LweI	GCATC	7 cut(s)	706, 854, 957, 1034, 1312, 1462, 1550
MaeI	CTAG	4 cut(s)	372, 590, 1796, 1857
MaeII	ACGT	2 cut(s)	2018, 2136
MaeIII	GTNAC	7 cut(s)	82, 302, 1346, 1718, 1740, 1770, 1798
MalI	GATC	3 cut(s)	697, 1087, 2166
MbiI	CCGCTC	1 cut(s)	442
MboI	GATC	3 cut(s)	695, 1085, 2164
MfeI	CAATTG	1 cut(s)	1905
MflI	RGATCY	3 cut(s)	695, 1085, 2164
MhlI	GDGCHC	5 cut(s)	12, 145, 984, 1001, 1848
MlsI	TGGCCA	1 cut(s)	1598
MluNI	TGGCCA	1 cut(s)	1598
MlyI	GAGTC	4 cut(s)	937, 1343, 1499, 2057
MmeI	TCCRAC	1 cut(s)	483
Mox20I	TGGCCA	1 cut(s)	1598
MroXI	GAANNNNTTC	1 cut(s)	2166
MscI	TGGCCA	1 cut(s)	1598
MslI	CAYNNNNRTG	1 cut(s)	1454
Msp20I	TGGCCA	1 cut(s)	1598
MspA1I	CMGCKG	2 cut(s)	1220, 2150
MspCI	CTTAAG	2 cut(s)	892, 1204
MspI	CCGG	1 cut(s)	1782
MspR9I	CCNGG	3 cut(s)	1405, 1421, 1595
MunI	CAATTG	1 cut(s)	1905
Mva1269I	GAATGC	1 cut(s)	916
MvaI	CCWGG	3 cut(s)	1405, 1421, 1595
MvnI	CGCG	2 cut(s)	245, 710
NcoI	CCATGG	1 cut(s)	1411
NdeI	CATATG	1 cut(s)	1123
NdeII	GATC	3 cut(s)	695, 1085, 2164
NheI	GCTAGC	1 cut(s)	371
NlaIII	CATG	9 cut(s)	242, 481, 748, 833, 1415, 1502, 1574, 1684, 2014
NlaIV	GGNNCC	7 cut(s)	286, 360, 697, 1409, 1417, 1608, 1668
NmuCI	GTSAC	5 cut(s)	82, 302, 1346, 1770, 1798
NspI	RCATGY	1 cut(s)	242
PaeR7I	CTCGAG	2 cut(s)	1736, 2142
PasI	CCCWGGG	1 cut(s)	1420
PceI	AGGCCT	1 cut(s)	1403
PctI	GAATGC	1 cut(s)	916
PdmI	GAANNNNTTC	1 cut(s)	2166
PfeI	GAWTC	4 cut(s)	49, 1792, 1989, 2066
PflFI	GACNNNGTC	1 cut(s)	2135
PkrI	GCNGC	9 cut(s)	154, 244, 844, 1106, 1302, 1477, 1912, 1915, 2149
PleI	GAGTC	4 cut(s)	936, 1343, 1499, 2056
PpsI	GAGTC	4 cut(s)	936, 1343, 1499, 2056
PshAI	GACNNNNGTC	1 cut(s)	1857
Psp124BI	GAGCTC	2 cut(s)	12, 1848
Psp6I	CCWGG	3 cut(s)	1403, 1419, 1593
PspEI	GGTNACC	1 cut(s)	302
PspGI	CCWGG	3 cut(s)	1403, 1419, 1593
PspN4I	GGNNCC	7 cut(s)	286, 360, 697, 1409, 1417, 1608, 1668
PspPI	GGNCC	3 cut(s)	284, 1415, 2099
PstNI	CAGNNNCTG	1 cut(s)	1046
PsuI	RGATCY	3 cut(s)	695, 1085, 2164
PsyI	GACNNNGTC	1 cut(s)	2135
PvuII	CAGCTG	2 cut(s)	1220, 2150
RseI	CAYNNNNRTG	1 cut(s)	1454
SacI	GAGCTC	2 cut(s)	12, 1848
SatI	GCNGC	9 cut(s)	153, 243, 843, 1105, 1301, 1476, 1911, 1914, 2148
Sau3AI	GATC	3 cut(s)	695, 1085, 2164
Sau96I	GGNCC	3 cut(s)	284, 1415, 2099
SchI	GAGTC	4 cut(s)	937, 1343, 1499, 2057
ScrFI	CCNGG	3 cut(s)	1405, 1421, 1595
SduI	GDGCHC	5 cut(s)	12, 145, 984, 1001, 1848
SfaNI	GCATC	7 cut(s)	706, 854, 957, 1034, 1312, 1462, 1550
SfcI	CTRYAG	4 cut(s)	312, 1221, 1336, 1761
Sfr274I	CTCGAG	2 cut(s)	1736, 2142
SinI	GGWCC	3 cut(s)	284, 1415, 2099
SlaI	CTCGAG	2 cut(s)	1736, 2142
SmiMI	CAYNNNNRTG	1 cut(s)	1454
SmlI	CTYRAG	5 cut(s)	892, 1204, 1556, 1736, 2142
SmoI	CTYRAG	5 cut(s)	892, 1204, 1556, 1736, 2142
SseBI	AGGCCT	1 cut(s)	1403
SsiI	CCGC	4 cut(s)	243, 440, 708, 1914
SspI	AATATT	3 cut(s)	21, 574, 667
SspMI	CTAG	4 cut(s)	372, 590, 1796, 1857
SstI	GAGCTC	2 cut(s)	12, 1848
StuI	AGGCCT	1 cut(s)	1403
StyD4I	CCNGG	3 cut(s)	1403, 1419, 1593
StyI	CCWWGG	1 cut(s)	1411
TaaI	ACNGT	6 cut(s)	399, 464, 512, 1063, 1072, 1718
TaiI	ACGT	2 cut(s)	2021, 2139
TaqI	TCGA	9 cut(s)	137, 322, 401, 671, 1111, 1737, 1808, 2084, 2143
TatI	WGTACW	1 cut(s)	643
TauI	GCSGC	2 cut(s)	245, 1916
TfiI	GAWTC	4 cut(s)	49, 1792, 1989, 2066
TscAI	CASTG	8 cut(s)	91, 100, 201, 515, 858, 1077, 1753, 1977
TseFI	GTSAC	5 cut(s)	82, 302, 1346, 1770, 1798
TseI	GCWGC	7 cut(s)	152, 842, 1104, 1300, 1475, 1910, 2147
Tsp45I	GTSAC	5 cut(s)	82, 302, 1346, 1770, 1798
TspDTI	ATGAA	8 cut(s)	62, 356, 593, 952, 1140, 1472, 1515, 1559
TspGWI	ACGGA	4 cut(s)	70, 995, 1737, 1895
TspRI	CASTG	8 cut(s)	91, 100, 201, 515, 858, 1077, 1753, 1977
Tth111I	GACNNNGTC	1 cut(s)	2135
Vha464I	CTTAAG	2 cut(s)	892, 1204
VneI	GTGCAC	2 cut(s)	141, 997
VpaK11BI	GGWCC	3 cut(s)	284, 1415, 2099
XceI	RCATGY	1 cut(s)	242
XhoI	CTCGAG	2 cut(s)	1736, 2142
XmnI	GAANNNNTTC	1 cut(s)	2166
XspI	CTAG	4 cut(s)	372, 590, 1796, 1857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.