Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009544

Physical Location & Seq

Genomic Coordinates Reverse (-)

33565689 .. 33571683

Gene Structure 5995 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1274000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGTCTGAGCTCACAGGAATATTGAGCAAGGTGCAATGGTTGTTGATGAATCAGTTGCTTGTGTTTTGTTATGTGATTTCCATCACTGAAGCGAGTGAACACTATGATCTACTCGAAGGACCGTCAGTATCTCCATCGAGTGCACCTTGGGCTGCATCAGTGTCCCACTCCCATAGACGCTGGCAAAAAAATTTCTTGCCACATGCTGCGCCAGTCGCTCTATCTCCCGCTCACCCTCCTTTTTATGGTCCCTTGATAACTGCTGGTCATCCCCCTACAGCTTCTCGATTGTCAAAGCTGTCAATGAAGAGGGGTGGCTTGGCACCTCCCCTTGCTGGCTTTAAGAATATCGCCCCAACACAGTCGAGTGCTGGTACAATTCCTTCTGGTTTAGCACAGCCCCCGCTCACTCCTTCCATTTCCAACTGTTGCAAACCAGACATGGTACTGAGACGGGGAAGTCAGGGTTGCCAATGTGCTTATCCCATAAAGCTTGATATTCTCCTCCTAAATGTTTCACAAAATCCTAACTGGAATCTTTTTCTTGAAGAACTAGCTTCCGAGCTTCATTTGCAAGTTTCTCAAATAGAGCTGATTAACTTTTATGTACTCGGATTATCAAGATTAAATATTTCAATGGATATTACTCCCAGTTCAGGACTCAGCTTCTCTGCAAGTGATGCGTCAGCAATAAACTCTTCTCTTGTCATGCACAAGATTAAATTGAACCCTGCATTAGTGGGCGATTACAAACTCCTCAATATAACTTGGTTTAAGCCTCCACCTCCTTCTCATGCTCCTGTTATTGCTGCATCACCAGTGGAAGCCCCAGCAAATCCATCCCCGACTGGTACATCCTCAAGTGCTTCAGATAAAGGGAGGCATTCAAATTGGAATCTCATTATTGGTATCGGCGTTGGCATACTGTTCATTGCCATTATATCTGTGCTCATACTTTGTTTGTGCACATTCCGCCGAGAGAAGACTAAAGCTTCTCCTATAGAAACAGCCCCTGAAAAGCAGATGACTGTTGATACAGTACCAGCAGTAGGACCTTTACCTCATCCAAGCAGCACTCGATTTCTGACATATGAAGAACTTAAACAAGCAACAAACAACTTTGAACCTGCAAGCATACTCGGAGAGGGTGGTTTTGGCAAGGTGTTCAAGGGTGTCTTAAGTGATGGTACAGCTGTAGCAATTAAAAGACTTACCAATGGAGGGCAACAGGGCGATAAGGAGTTCTTGGTTGAGGTTGAAATGCTGAGCAGGCTGCATCATCGTAATCTGGTGAAACTTGTGGGTTACTATAGCAGTCGTGACTCTTCACAAAACTTACTTTGCTATGAGCTTGTACCAAATGGAAGCTTGGAGGCCTGGCTCCATGGTCCCCTGGGAGTAAATTGTCCTTTGGATTGGGACACCCGAATGAAAATTGCACTTGATGCTGCCAGAGGACTTGCTTACCTGCATGAGGACTCACAACCTTGTGTCATCCACAGAGATTTTAAAGCATCTAATATATTGCTTGAGAACAACTTTCATGCTAAAGTTGCTGATTTTGGCCTGGCTAAACAGGCTCCTGAAGGCAGAGCAAACTATCTTTCAACTCGTGTGATGGGAACATTCGGGTATGTGGCTCCAGAGTACGCCATGACTGGACACCTACTTGTTAAAAGTGATGTTTACAGTTATGGGGTTGTCCTTCTTGAGTTACTCACTGGAAGAAAGCCTGTAGATATGTCACAGCCAGCCGGACAGGAGAATCTTGTCACTTGGTCAAGGCCAATTCTTAGAGACAAGGATAGGCTGGATGAGCTTGCTGATCCAAGTCTTGAAGGAAAGTACCCGACGGAAGACTTTGTACGAGTTTGCACGATTGCAGCGGCTTGTGTTGCTCCTGAGGCAAGCCAACGCCCTACGATGGGTGAAGTGGTACAATCACTGAAGATGGTGCAACGGATCACAGAATACCAGGATTCCATGTTAACCTCTTCCACTACCAGACCCAATAACAGGCAGTCTTCAACTACCTACGAATCCGATGTGTCATCTTCAATGTTCTCTTCTGGTCCTTACTCTGGTCTAAGTGGCTTTGATAATGACAACGTCTCTCGAGCAGCAGTGTTCTCTGAAGATCTTCACGAAGGACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

719

Amino Acids

78.02

Weight (kDa)

6.56

Isoelectric Point (pI)

50.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	155 - 257	3.2e-07	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	376 - 647	9.9e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	378 - 649	6.5e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009544)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02010
malus_domestica	MD09G1283200.v1.1 MD17G1274000.v1.1
prunus_persica	Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1
pyrus_communis	pycom09g19120 pycom17g27200
rosa_chinensis	RchiOBHm_Chr3g0487601
rosa_laevigata	RLG00000022997
rosa_multiflora	Rmu_sc0005285.1_g000001 Rmu_sc0012412.1_g000008
rosa_roxburghii	Rroxscaffold_6G00394660
rosa_rugosa	Rorug03G0234600 Rorug03G0234700 Rorug03G0234800 Rorug03G0234900
rosa_samantha	Rh3AG283200 Rh3BG318900 Rh3CG317000 Rh3DG315200
rosa_wichuraiana	Rw3G025040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	459
Acc36I	ACCTGC	2 cut(s)	1135, 1476
AccB1I	GGYRCC	1 cut(s)	322
AccBSI	CCGCTC	2 cut(s)	230, 406
AciI	CCGC	4 cut(s)	228, 404, 973, 1886
AclWI	GGATC	2 cut(s)	1820, 1970
AcsI	RAATTY	1 cut(s)	190
AcuI	CTGAAG	5 cut(s)	108, 852, 1605, 1967, 2154
AflII	CTTAAG	1 cut(s)	1177
AjnI	CCWGG	4 cut(s)	1376, 1392, 1566, 1974
Alw21I	GWGCWC	4 cut(s)	12, 145, 951, 968
Alw26I	GTCTC	3 cut(s)	445, 1791, 2116
Alw44I	GTGCAC	2 cut(s)	141, 964
AlwI	GGATC	2 cut(s)	1820, 1970
AlwNI	CAGNNNCTG	1 cut(s)	1013
Ama87I	CYCGRG	1 cut(s)	2115
AoxI	GGCC	3 cut(s)	1374, 1564, 1784
ApaLI	GTGCAC	2 cut(s)	141, 964
ApeKI	GCWGC	8 cut(s)	152, 206, 809, 1071, 1273, 1448, 1883, 2120
ApoI	RAATTY	1 cut(s)	190
Asp700I	GAANNNNTTC	1 cut(s)	2139
AspLEI	GCGC	1 cut(s)	211
AspS9I	GGNCC	5 cut(s)	119, 248, 1052, 1388, 2072
AsuHPI	GGTGA	4 cut(s)	224, 807, 1303, 1940
AvaI	CYCGRG	1 cut(s)	2115
AvaII	GGWCC	5 cut(s)	119, 248, 1052, 1388, 2072
AxyI	CCTNAGG	1 cut(s)	1902
BaeGI	GKGCMC	2 cut(s)	145, 968
BaeI	ACNNNNGTAYC	2 cut(s)	1179, 1212
BanI	GGYRCC	1 cut(s)	322
BanII	GRGCYC	1 cut(s)	12
BarI	GAAGNNNNNNTAC	4 cut(s)	367, 399, 1848, 1880
BauI	CACGAG	1 cut(s)	1611
BbsI	GAAGAC	3 cut(s)	989, 1863, 2016
Bbv12I	GWGCWC	4 cut(s)	12, 145, 951, 968
BbvI	GCAGC	8 cut(s)	139, 193, 796, 1083, 1260, 1435, 1895, 2132
BccI	CCATC	7 cut(s)	89, 142, 847, 1178, 1612, 1918, 1945
BciT130I	CCWGG	4 cut(s)	1378, 1394, 1568, 1976
BcoDI	GTCTC	3 cut(s)	445, 1791, 2116
BfaI	CTAG	1 cut(s)	554
BfmI	CTRYAG	5 cut(s)	276, 999, 1194, 1309, 1734
BfrI	CTTAAG	1 cut(s)	1177
BfuAI	ACCTGC	2 cut(s)	1135, 1476
BglII	AGATCT	1 cut(s)	2137
BisI	GCNGC	9 cut(s)	153, 207, 810, 1072, 1274, 1449, 1884, 1887, 2121
BlpI	GCTNAGC	1 cut(s)	1265
BlsI	GCNGC	9 cut(s)	154, 208, 811, 1073, 1275, 1450, 1885, 1888, 2122
Bme1390I	CCNGG	4 cut(s)	1378, 1394, 1568, 1976
Bme18I	GGWCC	5 cut(s)	119, 248, 1052, 1388, 2072
BmeT110I	CYCGRG	1 cut(s)	2115
BmgT120I	GGNCC	5 cut(s)	119, 248, 1052, 1388, 2072
BmiI	GGNNCC	6 cut(s)	250, 324, 1382, 1390, 1581, 1641
BmrFI	CCNGG	4 cut(s)	1378, 1394, 1568, 1976
BmrI	ACTGGG	1 cut(s)	645
BmsI	GCATC	6 cut(s)	164, 670, 821, 1285, 1435, 1523
BmuI	ACTGGG	1 cut(s)	645
BpiI	GAAGAC	3 cut(s)	989, 1863, 2016
BpmI	CTGGAG	1 cut(s)	1626
Bpu1102I	GCTNAGC	1 cut(s)	1265
BpuEI	CTTGAG	3 cut(s)	844, 1550, 1730
BsaBI	GATNNNNATC	1 cut(s)	80
BsaJI	CCNNGG	4 cut(s)	146, 1384, 1392, 1393
Bse1I	ACTGG	7 cut(s)	212, 536, 651, 818, 853, 1663, 1726
Bse21I	CCTNAGG	1 cut(s)	1902
Bse3DI	GCAATG	2 cut(s)	41, 930
Bse8I	GATNNNNATC	1 cut(s)	80
BseBI	CCWGG	4 cut(s)	1378, 1394, 1568, 1976
BseDI	CCNNGG	4 cut(s)	146, 1384, 1392, 1393
BseGI	GGATG	6 cut(s)	268, 839, 854, 1063, 1494, 1819
BseJI	GATNNNNATC	1 cut(s)	80
BseMI	GCAATG	2 cut(s)	41, 930
BseMII	CTCAG	4 cut(s)	440, 676, 1256, 1893
BseNI	ACTGG	7 cut(s)	212, 536, 651, 818, 853, 1663, 1726
BseRI	GAGGAG	2 cut(s)	494, 746
BseSI	GKGCMC	2 cut(s)	145, 968
BseXI	GCAGC	8 cut(s)	139, 193, 796, 1083, 1260, 1435, 1895, 2132
BseYI	CCCAGC	1 cut(s)	829
BshFI	GGCC	3 cut(s)	1376, 1566, 1786
BshNI	GGYRCC	1 cut(s)	322
BsiHKAI	GWGCWC	4 cut(s)	12, 145, 951, 968
BsiHKCI	CYCGRG	1 cut(s)	2115
BsiSI	CCGG	1 cut(s)	1755
BslFI	GGGAC	4 cut(s)	148, 234, 1374, 1433
BsmAI	GTCTC	3 cut(s)	445, 1791, 2116
BsmBI	CGTCTC	2 cut(s)	445, 2116
BsmFI	GGGAC	4 cut(s)	148, 234, 1374, 1433
BsmI	GAATGC	1 cut(s)	883
BsnI	GGCC	3 cut(s)	1376, 1566, 1786
BsoBI	CYCGRG	1 cut(s)	2115
Bsp1286I	GDGCHC	4 cut(s)	12, 145, 951, 968
Bsp143I	GATC	4 cut(s)	106, 1825, 1962, 2137
Bsp1720I	GCTNAGC	1 cut(s)	1265
Bsp19I	CCATGG	1 cut(s)	1384
BspACI	CCGC	4 cut(s)	228, 404, 973, 1886
BspANI	GGCC	3 cut(s)	1376, 1566, 1786
BspCNI	CTCAG	4 cut(s)	441, 675, 1257, 1894
BspLI	GGNNCC	6 cut(s)	250, 324, 1382, 1390, 1581, 1641
BspMI	ACCTGC	2 cut(s)	1135, 1476
BspPI	GGATC	2 cut(s)	1820, 1970
BspT107I	GGYRCC	1 cut(s)	322
BspTI	CTTAAG	1 cut(s)	1177
BsrBI	CCGCTC	2 cut(s)	230, 406
BsrDI	GCAATG	2 cut(s)	41, 930
BsrI	ACTGG	7 cut(s)	212, 536, 651, 818, 853, 1663, 1726
BssECI	CCNNGG	4 cut(s)	146, 1384, 1392, 1393
BssMI	GATC	4 cut(s)	106, 1825, 1962, 2137
BssSI	CACGAG	1 cut(s)	1611
BssT1I	CCWWGG	2 cut(s)	146, 1384
Bst2BI	CACGAG	1 cut(s)	1611
Bst2UI	CCWGG	4 cut(s)	1378, 1394, 1568, 1976
Bst4CI	ACNGT	7 cut(s)	123, 363, 428, 927, 1030, 1039, 1691
Bst6I	CTCTTC	5 cut(s)	302, 703, 1330, 1999, 2071
BstAFI	CTTAAG	1 cut(s)	1177
BstAPI	GCANNNNNTGC	2 cut(s)	680, 1445
BstC8I	GCNNGC	7 cut(s)	182, 337, 1132, 1271, 1752, 1821, 1909
BstDEI	CTNAG	7 cut(s)	6, 449, 662, 1265, 1793, 1902, 2087
BstDSI	CCRYGG	1 cut(s)	1384
BstENI	CCTNNNNNAGG	1 cut(s)	1472
BstF5I	GGATG	6 cut(s)	268, 839, 854, 1063, 1494, 1819
BstHHI	GCGC	1 cut(s)	211
BstKTI	GATC	4 cut(s)	109, 1828, 1965, 2140
BstMAI	GTCTC	3 cut(s)	445, 1791, 2116
BstMBI	GATC	4 cut(s)	106, 1825, 1962, 2137
BstNI	CCWGG	4 cut(s)	1378, 1394, 1568, 1976
BstNSI	RCATGY	1 cut(s)	206
BstSCI	CCNGG	4 cut(s)	1376, 1392, 1566, 1974
BstSFI	CTRYAG	5 cut(s)	276, 999, 1194, 1309, 1734
BstSLI	GKGCMC	2 cut(s)	145, 968
BstV1I	GCAGC	8 cut(s)	139, 193, 796, 1083, 1260, 1435, 1895, 2132
BstV2I	GAAGAC	3 cut(s)	989, 1863, 2016
BstX2I	RGATCY	1 cut(s)	2137
BstYI	RGATCY	1 cut(s)	2137
Bsu36I	CCTNAGG	1 cut(s)	1902
BsuRI	GGCC	3 cut(s)	1376, 1566, 1786
BtgI	CCRYGG	1 cut(s)	1384
BtsCI	GGATG	6 cut(s)	268, 839, 854, 1063, 1494, 1819
BtsI	GCAGTG	1 cut(s)	2130
BtsIMutI	CAGTG	6 cut(s)	84, 165, 825, 1719, 1943, 2130
BveI	ACCTGC	2 cut(s)	1135, 1476
Cac8I	GCNNGC	7 cut(s)	182, 337, 1132, 1271, 1752, 1821, 1909
CaiI	CAGNNNCTG	1 cut(s)	1013
CfoI	GCGC	1 cut(s)	211
Cfr13I	GGNCC	5 cut(s)	119, 248, 1052, 1388, 2072
CseI	GACGC	2 cut(s)	186, 672
CviAII	CATG	9 cut(s)	203, 442, 709, 794, 1385, 1472, 1544, 1654, 1984
DdeI	CTNAG	7 cut(s)	6, 449, 662, 1265, 1793, 1902, 2087
DpnI	GATC	4 cut(s)	108, 1827, 1964, 2139
DpnII	GATC	4 cut(s)	106, 1825, 1962, 2137
DraI	TTTAAA	1 cut(s)	1510
DrdI	GACNNNNNNGTC	1 cut(s)	459
DseDI	GACNNNNNNGTC	1 cut(s)	459
Eam1104I	CTCTTC	5 cut(s)	302, 703, 1330, 1999, 2071
EarI	CTCTTC	5 cut(s)	302, 703, 1330, 1999, 2071
EciI	GGCGGA	1 cut(s)	962
Ecl136II	GAGCTC	1 cut(s)	10
Eco130I	CCWWGG	2 cut(s)	146, 1384
Eco147I	AGGCCT	1 cut(s)	1376
Eco24I	GRGCYC	1 cut(s)	12
Eco47I	GGWCC	5 cut(s)	119, 248, 1052, 1388, 2072
Eco53kI	GAGCTC	1 cut(s)	10
Eco57I	CTGAAG	5 cut(s)	108, 852, 1605, 1967, 2154
Eco81I	CCTNAGG	1 cut(s)	1902
Eco88I	CYCGRG	1 cut(s)	2115
EcoICRI	GAGCTC	1 cut(s)	10
EcoNI	CCTNNNNNAGG	1 cut(s)	1472
EcoO109I	RGGNCCY	1 cut(s)	1052
EcoRII	CCWGG	4 cut(s)	1376, 1392, 1566, 1974
EcoT14I	CCWWGG	2 cut(s)	146, 1384
EcoT38I	GRGCYC	1 cut(s)	12
ErhI	CCWWGG	2 cut(s)	146, 1384
Esp3I	CGTCTC	2 cut(s)	445, 2116
FaeI	CATG	9 cut(s)	206, 445, 712, 797, 1388, 1475, 1547, 1657, 1987
FalI	AAGNNNNNCTT	4 cut(s)	1160, 1192, 1229, 1261
FaqI	GGGAC	4 cut(s)	148, 234, 1374, 1433
FatI	CATG	9 cut(s)	202, 441, 708, 793, 1384, 1471, 1543, 1653, 1983
FauI	CCCGC	2 cut(s)	235, 411
FauNDI	CATATG	1 cut(s)	1090
Fnu4HI	GCNGC	9 cut(s)	153, 207, 810, 1072, 1274, 1449, 1884, 1887, 2121
FokI	GGATG	6 cut(s)	255, 826, 841, 1050, 1481, 1826
FriOI	GRGCYC	1 cut(s)	12
Fsp4HI	GCNGC	9 cut(s)	153, 207, 810, 1072, 1274, 1449, 1884, 1887, 2121
FspBI	CTAG	1 cut(s)	554
GlaI	GCGC	1 cut(s)	210
GluI	GCNGC	9 cut(s)	153, 207, 810, 1072, 1274, 1449, 1884, 1887, 2121
GsaI	CCCAGC	1 cut(s)	833
GsuI	CTGGAG	1 cut(s)	1626
HaeIII	GGCC	3 cut(s)	1376, 1566, 1786
HapII	CCGG	1 cut(s)	1755
HgaI	GACGC	2 cut(s)	186, 672
HhaI	GCGC	1 cut(s)	211
Hin1II	CATG	9 cut(s)	206, 445, 712, 797, 1388, 1475, 1547, 1657, 1987
Hin6I	GCGC	1 cut(s)	209
HinP1I	GCGC	1 cut(s)	209
HincII	GTYRAC	1 cut(s)	1989
HindII	GTYRAC	1 cut(s)	1989
HindIII	AAGCTT	3 cut(s)	491, 990, 1366
HinfI	GANTC	9 cut(s)	49, 535, 660, 895, 1322, 1478, 1765, 1979, 2039
HpaI	GTTAAC	1 cut(s)	1989
HpaII	CCGG	1 cut(s)	1755
HphI	GGTGA	4 cut(s)	224, 807, 1303, 1940
Hpy166II	GTNNAC	5 cut(s)	98, 143, 966, 1687, 1989
Hpy188I	TCNGA	8 cut(s)	7, 562, 614, 871, 1086, 1142, 2044, 2134
Hpy8I	GTNNAC	5 cut(s)	98, 143, 966, 1687, 1989
Hpy99I	CGWCG	1 cut(s)	1855
HpyAV	CCTTC	8 cut(s)	110, 393, 423, 798, 1580, 1715, 1832, 2141
HpyCH4III	ACNGT	7 cut(s)	123, 363, 428, 927, 1030, 1039, 1691
HpyCH4IV	ACGT	1 cut(s)	2109
HpyF3I	CTNAG	7 cut(s)	6, 449, 662, 1265, 1793, 1902, 2087
HpySE526I	ACGT	1 cut(s)	2109
Hsp92II	CATG	9 cut(s)	206, 445, 712, 797, 1388, 1475, 1547, 1657, 1987
HspAI	GCGC	1 cut(s)	209
KspAI	GTTAAC	1 cut(s)	1989
Kzo9I	GATC	4 cut(s)	106, 1825, 1962, 2137
LmnI	GCTCC	5 cut(s)	802, 1386, 1585, 1645, 1903
Lsp1109I	GCAGC	8 cut(s)	139, 193, 796, 1083, 1260, 1435, 1895, 2132
LweI	GCATC	6 cut(s)	164, 670, 821, 1285, 1435, 1523
MaeI	CTAG	1 cut(s)	554
MaeII	ACGT	1 cut(s)	2109
MaeIII	GTNAC	5 cut(s)	1304, 1319, 1713, 1743, 1771
MalI	GATC	4 cut(s)	108, 1827, 1964, 2139
MbiI	CCGCTC	2 cut(s)	230, 406
MboI	GATC	4 cut(s)	106, 1825, 1962, 2137
MflI	RGATCY	1 cut(s)	2137
MhlI	GDGCHC	4 cut(s)	12, 145, 951, 968
MluCI	AATT	8 cut(s)	190, 378, 722, 889, 1200, 1402, 1434, 1788
MlyI	GAGTC	3 cut(s)	654, 1316, 1472
MmeI	TCCRAC	1 cut(s)	447
MroXI	GAANNNNTTC	1 cut(s)	2139
MslI	CAYNNNNRTG	1 cut(s)	1427
MspA1I	CMGCKG	2 cut(s)	1193, 1886
MspCI	CTTAAG	1 cut(s)	1177
MspI	CCGG	1 cut(s)	1755
MspR9I	CCNGG	4 cut(s)	1378, 1394, 1568, 1976
Mva1269I	GAATGC	1 cut(s)	883
MvaI	CCWGG	4 cut(s)	1378, 1394, 1568, 1976
NcoI	CCATGG	1 cut(s)	1384
NdeI	CATATG	1 cut(s)	1090
NdeII	GATC	4 cut(s)	106, 1825, 1962, 2137
NlaIII	CATG	9 cut(s)	206, 445, 712, 797, 1388, 1475, 1547, 1657, 1987
NlaIV	GGNNCC	6 cut(s)	250, 324, 1382, 1390, 1581, 1641
NmeAIII	GCCGAG	1 cut(s)	1001
NmuCI	GTSAC	3 cut(s)	1319, 1743, 1771
NspI	RCATGY	1 cut(s)	206
PaeR7I	CTCGAG	1 cut(s)	2115
PasI	CCCWGGG	1 cut(s)	1393
PceI	AGGCCT	1 cut(s)	1376
PctI	GAATGC	1 cut(s)	883
PdmI	GAANNNNTTC	1 cut(s)	2139
PfeI	GAWTC	6 cut(s)	49, 535, 895, 1765, 1979, 2039
PflFI	GACNNNGTC	1 cut(s)	2108
PkrI	GCNGC	9 cut(s)	154, 208, 811, 1073, 1275, 1450, 1885, 1888, 2122
PleI	GAGTC	3 cut(s)	654, 1316, 1472
PpsI	GAGTC	3 cut(s)	654, 1316, 1472
PpuMI	RGGWCCY	1 cut(s)	1052
Psp124BI	GAGCTC	1 cut(s)	12
Psp5II	RGGWCCY	1 cut(s)	1052
Psp6I	CCWGG	4 cut(s)	1376, 1392, 1566, 1974
PspFI	CCCAGC	1 cut(s)	829
PspGI	CCWGG	4 cut(s)	1376, 1392, 1566, 1974
PspN4I	GGNNCC	6 cut(s)	250, 324, 1382, 1390, 1581, 1641
PspPI	GGNCC	5 cut(s)	119, 248, 1052, 1388, 2072
PspPPI	RGGWCCY	1 cut(s)	1052
PstNI	CAGNNNCTG	1 cut(s)	1013
PsuI	RGATCY	1 cut(s)	2137
PsyI	GACNNNGTC	1 cut(s)	2108
PvuII	CAGCTG	1 cut(s)	1193
RseI	CAYNNNNRTG	1 cut(s)	1427
SacI	GAGCTC	1 cut(s)	12
SatI	GCNGC	9 cut(s)	153, 207, 810, 1072, 1274, 1449, 1884, 1887, 2121
Sau3AI	GATC	4 cut(s)	106, 1825, 1962, 2137
Sau96I	GGNCC	5 cut(s)	119, 248, 1052, 1388, 2072
SchI	GAGTC	3 cut(s)	654, 1316, 1472
ScrFI	CCNGG	4 cut(s)	1378, 1394, 1568, 1976
SduI	GDGCHC	4 cut(s)	12, 145, 951, 968
SfaNI	GCATC	6 cut(s)	164, 670, 821, 1285, 1435, 1523
SfcI	CTRYAG	5 cut(s)	276, 999, 1194, 1309, 1734
Sfr274I	CTCGAG	1 cut(s)	2115
SinI	GGWCC	5 cut(s)	119, 248, 1052, 1388, 2072
SlaI	CTCGAG	1 cut(s)	2115
SmiMI	CAYNNNNRTG	1 cut(s)	1427
SmlI	CTYRAG	5 cut(s)	859, 1177, 1529, 1709, 2115
SmoI	CTYRAG	5 cut(s)	859, 1177, 1529, 1709, 2115
Sse9I	AATT	8 cut(s)	190, 378, 722, 889, 1200, 1402, 1434, 1788
SseBI	AGGCCT	1 cut(s)	1376
SsiI	CCGC	4 cut(s)	228, 404, 973, 1886
SspI	AATATT	2 cut(s)	21, 631
SspMI	CTAG	1 cut(s)	554
SstI	GAGCTC	1 cut(s)	12
StuI	AGGCCT	1 cut(s)	1376
StyD4I	CCNGG	4 cut(s)	1376, 1392, 1566, 1974
StyI	CCWWGG	2 cut(s)	146, 1384
TaaI	ACNGT	7 cut(s)	123, 363, 428, 927, 1030, 1039, 1691
TaiI	ACGT	1 cut(s)	2112
TaqI	TCGA	6 cut(s)	114, 137, 286, 365, 1078, 2116
TasI	AATT	8 cut(s)	190, 378, 722, 889, 1200, 1402, 1434, 1788
TatI	WGTACW	1 cut(s)	607
TauI	GCSGC	1 cut(s)	1889
TfiI	GAWTC	6 cut(s)	49, 535, 895, 1765, 1979, 2039
TscAI	CASTG	6 cut(s)	91, 165, 825, 1726, 1950, 2130
TseFI	GTSAC	3 cut(s)	1319, 1743, 1771
TseI	GCWGC	8 cut(s)	152, 206, 809, 1071, 1273, 1448, 1883, 2120
Tsp45I	GTSAC	3 cut(s)	1319, 1743, 1771
TspDTI	ATGAA	7 cut(s)	62, 320, 557, 919, 1107, 1445, 1532
TspGWI	ACGGA	2 cut(s)	1868, 1975
TspRI	CASTG	6 cut(s)	91, 165, 825, 1726, 1950, 2130
Tth111I	GACNNNGTC	1 cut(s)	2108
Vha464I	CTTAAG	1 cut(s)	1177
VneI	GTGCAC	2 cut(s)	141, 964
VpaK11BI	GGWCC	5 cut(s)	119, 248, 1052, 1388, 2072
XagI	CCTNNNNNAGG	1 cut(s)	1472
XapI	RAATTY	1 cut(s)	190
XceI	RCATGY	1 cut(s)	206
XhoI	CTCGAG	1 cut(s)	2115
XmnI	GAANNNNTTC	1 cut(s)	2139
XspI	CTAG	1 cut(s)	554

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.