Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0009544

Physical Location & Seq

Genomic Coordinates Reverse (-)

15436635 .. 15443493

Gene Structure 6859 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00394660.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGGGGCAGATCCAGTTCAATCGTCCAGGGTCACTGAACAAGGTGCTGATGAAACAGTTGCTTTTGCTTTGTTGTGTGATATCTGTCGCTCATGCCAATGTACCCTATGATTTACACAAAGCATCCTCAGTATCTTTGACAAGTGCACCTTTGGCAGCACCTCCTATGCCAAATCTTCCTTTGCCAGCCAGGTTGCAACATTTCGTGCCGCGTGGTGCTCCCATGGCATTTTCACCTGCTCCTCTAAATGGCCCCTTGATAACTGCTGCTACTCCACCAACAACTTCTCAATTGGCGAAGCCACCAAAGAGCAGTGGCTTAGCACCTCCTTTTGCTGGCTTTAAGAGTATTGCTCCTCAGTCGATTGCTGGTTCAATTCCTCCTGGTTTAGCTAAGCCACCAATCTCTCCATCCAGTTCCAACTGTTGCAAACAGAATATGGTACTGAGACGAGGAAGTCAGGGTTGCAACTGCGTTTATCCCATAAAGCTTGACATTCTCCTGTTAAATGTTTCAACAAATCCTAATTGGAAGCTCTTTCTTGACGACTTTGCTAAGCAGCTTGGTCTGGAAGTTTCTCAAATAGAGCTGATTAACTTTTATGTACTCAACTATGCAAGATTAAATATTTCAATGGATATTACTCCACATTCAGGGGTCAGTTTCTCTGCCAGTGATGCATCTGCAATAAACTCTTCTCTTGTCATGCACAAGGTTAATTTAGACCCTTCATTAGTGGGTGATTACAAACTCCTCAATATCACTTGGTTTAAGCCTCCACCTCCTTCTCAAGCTCCTGTTGCCACATCACCATTGGAAACCCCACCAAAACCATCACCAGCTCTTACATCATCAAGTGCTTCAAATAAAGGGAAACATTCAAATTGGATTCATATTATTGGTATTGGTGCCGGCATACTGTTCCTTGCCATTATATCTGTGCTTATACTTTGTTTATGCATATTCCGTAAAGAGAAGATTAAAGCATCTCCTGCAGAAACAGCACCAGAGAAAGAGAGGACTGTTGACACAGTTCTACCAGTAGGATCTCTACCCCACCTTAGCAGCACTCGGTTTCTGGCATATGAAGAACTTAAACAAGCAACGAACAACTTTGAATCTGCAAGTATACTTGGAGAGGGTGGGTTTGGCAGGGTTTTCAAGGGTGTTTTAAGTGATGGTACAGCTGTAGCAATTAAAAGACTTACTAATGGAGGGCAACAGGGTGACAAGGAGTTCTTGGTGGAGGTTGAGATGCTTAGCAGGCTGCATCATCGTAATCTTGTGAAGCTTATCGGTTACTATAGCAGTCGTGACTCCTCACAAAACTTACTTTGCTATGAGCTTGTGCCAAATGGAAGCTTGGAGGCTTGGCTTCATGGTCCCCTGGGAGTGAATTGTCGTTTGGATTGGGACACCAGAATGAAGATTGCACTTGATGCTGCCAGAGGACTTGCTTACCTGCACGAGGACTCACAACCTTGTGTTATTCACAGAGATTTTAAAGCATCCAATATATTGCTGGAGAATGATTTTCATGCCAAAGTTGCTGATTTTGGCCTGGCTAAACAGGCACCTGAAGGCAGAGCAAATTACCTTTCTACTCGGGTCATGGGCACATTTGGGTATGTTGCTCCAGAGTACGCCATGACCGGACACCTACTAGTTAAAAGTGATGTTTACAGCTATGGGGTTGTCCTTCTTGAATTACTTACTGGAAGAAGGCCTGTAGATATGTCACAGCCATCTGGGCAGGAGAATCTTGTCACTTGGGCAAGGCCAATTCTTAGAGACAAGGATCGGCTGGAAGAGCTTGCTGATCCTAGTCTTGGAGCAAAGTACCCAATGGAAGATTTTGTAAGGGTTTGCACCATTGCAGCAGCCTGTGTTGCTCCTGAGGCAAGCCAACGCCCCACAATGGGTGAAGTGGTACAGTCACTGAAGATGGTTCAAAGGATCACTGAATATCAGGATTCTGCGTTAACCTCTTCCACTGCCAGACCCAATCATAGGTCATCAACTACCTTTGAGTCTGATGTATCATCCTCAATGTTCTCTTCTGGTCCATACTCTGGTCTAAGTGCGTTTGATAATGACAACATCTCCCGGACAGCATTTTTTTCTGAAGATCTTCATGAAGGACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

715

Amino Acids

77.52

Weight (kDa)

8.43

Isoelectric Point (pI)

47.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	154 - 256	7.8e-08	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	373 - 645	8.2e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	376 - 647	6.6e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009544)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02010
malus_domestica	MD09G1283200.v1.1 MD17G1274000.v1.1
prunus_persica	Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1 Prupe.3G011000_v2.0.a1
pyrus_communis	pycom09g19120 pycom17g27200
rosa_chinensis	RchiOBHm_Chr3g0487601
rosa_laevigata	RLG00000022997
rosa_multiflora	Rmu_sc0005285.1_g000001 Rmu_sc0012412.1_g000008
rosa_roxburghii	Rroxscaffold_6G00394660
rosa_rugosa	Rorug03G0234600 Rorug03G0234700 Rorug03G0234800 Rorug03G0234900
rosa_samantha	Rh3AG283200 Rh3BG318900 Rh3CG317000 Rh3DG315200
rosa_wichuraiana	Rw3G025040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	244
AasI	GACNNNNNNGTC	1 cut(s)	456
Acc36I	ACCTGC	2 cut(s)	244, 1470
AccB1I	GGYRCC	2 cut(s)	908, 1573
AccB7I	CCANNNNNTGG	1 cut(s)	2072
AccI	GTMKAC	1 cut(s)	1129
AccII	CGCG	1 cut(s)	211
AciI	CCGC	1 cut(s)	209
AclWI	GGATC	5 cut(s)	4, 1054, 1806, 1814, 1964
AcuI	CTGAAG	3 cut(s)	1599, 1961, 2145
AfaI	GTAC	7 cut(s)	102, 444, 606, 1183, 1643, 1841, 1932
AfiI	CCNNNNNNNGG	9 cut(s)	248, 335, 653, 1468, 1829, 1918, 1919, 2010, 2072
AhlI	ACTAGT	1 cut(s)	1663
AjnI	CCWGG	5 cut(s)	25, 188, 382, 1386, 1560
AjuI	GAANNNNNNNTTGG	2 cut(s)	1388, 1420
Alw21I	GWGCWC	2 cut(s)	148, 220
Alw26I	GTCTC	2 cut(s)	442, 1785
Alw44I	GTGCAC	1 cut(s)	144
AlwI	GGATC	5 cut(s)	4, 1054, 1806, 1814, 1964
AlwNI	CAGNNNCTG	1 cut(s)	1939
Ama87I	CYCGRG	1 cut(s)	1605
AoxI	GGCC	4 cut(s)	250, 1558, 1724, 1778
ApaLI	GTGCAC	1 cut(s)	144
ApeKI	GCWGC	8 cut(s)	155, 266, 559, 1065, 1267, 1442, 1877, 1880
Asp700I	GAANNNNTTC	1 cut(s)	2130
AspS9I	GGNCC	3 cut(s)	251, 1382, 2063
AsuC2I	CCSGG	1 cut(s)	2107
AsuHPI	GGTGA	6 cut(s)	225, 752, 801, 828, 1238, 1934
AvaI	CYCGRG	1 cut(s)	1605
AvaII	GGWCC	2 cut(s)	1382, 2063
AxyI	CCTNAGG	1 cut(s)	1896
BaeGI	GKGCMC	2 cut(s)	148, 1619
BaeI	ACNNNNGTAYC	2 cut(s)	1173, 1206
BanI	GGYRCC	2 cut(s)	908, 1573
BauI	CACGAG	1 cut(s)	1466
Bbv12I	GWGCWC	2 cut(s)	148, 220
BbvI	GCAGC	8 cut(s)	167, 253, 571, 1077, 1254, 1429, 1889, 1892
BccI	CCATC	5 cut(s)	418, 841, 1172, 1753, 1939
BciT130I	CCWGG	5 cut(s)	27, 190, 384, 1388, 1562
BcnI	CCSGG	1 cut(s)	2107
BcoDI	GTCTC	2 cut(s)	442, 1785
BcuI	ACTAGT	1 cut(s)	1663
BfaI	CTAG	2 cut(s)	1664, 1824
BfmI	CTRYAG	4 cut(s)	993, 1188, 1303, 1728
BfuAI	ACCTGC	2 cut(s)	244, 1470
BglI	GCCNNNNNGGC	1 cut(s)	1750
BglII	AGATCT	1 cut(s)	2128
BisI	GCNGC	9 cut(s)	156, 209, 267, 560, 1066, 1268, 1443, 1878, 1881
BlpI	GCTNAGC	4 cut(s)	319, 393, 555, 1259
BlsI	GCNGC	9 cut(s)	157, 210, 268, 561, 1067, 1269, 1444, 1879, 1882
Bme1390I	CCNGG	6 cut(s)	27, 190, 384, 1388, 1562, 2107
Bme18I	GGWCC	2 cut(s)	1382, 2063
BmeT110I	CYCGRG	1 cut(s)	1605
BmgT120I	GGNCC	3 cut(s)	251, 1382, 2063
BmiI	GGNNCC	4 cut(s)	253, 910, 1384, 1575
BmrFI	CCNGG	6 cut(s)	27, 190, 384, 1388, 1562, 2107
BmsI	GCATC	8 cut(s)	131, 667, 689, 995, 1245, 1279, 1429, 1517
BplI	GAGNNNNNCTC	2 cut(s)	337, 369
BpmI	CTGGAG	2 cut(s)	1544, 1620
Bpu10I	CCTNAGC	1 cut(s)	1061
Bpu1102I	GCTNAGC	4 cut(s)	319, 393, 555, 1259
BpuEI	CTTGAG	1 cut(s)	774
BpuMI	CCSGG	1 cut(s)	2107
BsaJI	CCNNGG	4 cut(s)	26, 222, 1386, 1387
BsaWI	WCCGGW	1 cut(s)	1652
BsaXI	ACNNNNNCTCC	2 cut(s)	2087, 2117
Bsc4I	CCNNNNNNNGG	9 cut(s)	248, 335, 653, 1468, 1829, 1918, 1919, 2010, 2072
Bse118I	RCCGGY	1 cut(s)	911
Bse1I	ACTGG	5 cut(s)	13, 414, 672, 1040, 1720
Bse21I	CCTNAGG	1 cut(s)	1896
Bse3DI	GCAATG	1 cut(s)	1872
BseBI	CCWGG	5 cut(s)	27, 190, 384, 1388, 1562
BseDI	CCNNGG	4 cut(s)	26, 222, 1386, 1387
BseGI	GGATG	4 cut(s)	122, 410, 1508, 2042
BseLI	CCNNNNNNNGG	9 cut(s)	248, 335, 653, 1468, 1829, 1918, 1919, 2010, 2072
BseMI	GCAATG	1 cut(s)	1872
BseMII	CTCAG	4 cut(s)	141, 371, 437, 1887
BseNI	ACTGG	5 cut(s)	13, 414, 672, 1040, 1720
BseRI	GAGGAG	4 cut(s)	231, 345, 743, 1309
BseSI	GKGCMC	2 cut(s)	148, 1619
BseXI	GCAGC	8 cut(s)	167, 253, 571, 1077, 1254, 1429, 1889, 1892
BsgI	GTGCAG	1 cut(s)	1448
Bsh1236I	CGCG	1 cut(s)	211
BshFI	GGCC	4 cut(s)	252, 1560, 1726, 1780
BshNI	GGYRCC	2 cut(s)	908, 1573
BsiHKAI	GWGCWC	2 cut(s)	148, 220
BsiHKCI	CYCGRG	1 cut(s)	1605
BsiSI	CCGG	3 cut(s)	912, 1653, 2107
BslFI	GGGAC	2 cut(s)	1368, 1427
BslI	CCNNNNNNNGG	9 cut(s)	248, 335, 653, 1468, 1829, 1918, 1919, 2010, 2072
BsmAI	GTCTC	2 cut(s)	442, 1785
BsmBI	CGTCTC	1 cut(s)	442
BsmFI	GGGAC	2 cut(s)	1368, 1427
BsnI	GGCC	4 cut(s)	252, 1560, 1726, 1780
BsoBI	CYCGRG	1 cut(s)	1605
Bsp1286I	GDGCHC	3 cut(s)	148, 220, 1619
Bsp143I	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
Bsp1720I	GCTNAGC	4 cut(s)	319, 393, 555, 1259
Bsp19I	CCATGG	1 cut(s)	222
BspACI	CCGC	1 cut(s)	209
BspANI	GGCC	4 cut(s)	252, 1560, 1726, 1780
BspCNI	CTCAG	4 cut(s)	140, 370, 438, 1888
BspFNI	CGCG	1 cut(s)	211
BspHI	TCATGA	1 cut(s)	2134
BspLI	GGNNCC	4 cut(s)	253, 910, 1384, 1575
BspMAI	CTGCAG	1 cut(s)	997
BspMI	ACCTGC	2 cut(s)	244, 1470
BspPI	GGATC	5 cut(s)	4, 1054, 1806, 1814, 1964
BspQI	GCTCTTC	1 cut(s)	1803
BspT107I	GGYRCC	2 cut(s)	908, 1573
BsrDI	GCAATG	1 cut(s)	1872
BsrFI	RCCGGY	1 cut(s)	911
BsrI	ACTGG	5 cut(s)	13, 414, 672, 1040, 1720
BssAI	RCCGGY	1 cut(s)	911
BssECI	CCNNGG	4 cut(s)	26, 222, 1386, 1387
BssMI	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
BssNAI	GTATAC	1 cut(s)	1130
BssSI	CACGAG	1 cut(s)	1466
BssT1I	CCWWGG	1 cut(s)	222
Bst1107I	GTATAC	1 cut(s)	1130
Bst2BI	CACGAG	1 cut(s)	1466
Bst2UI	CCWGG	5 cut(s)	27, 190, 384, 1388, 1562
Bst4CI	ACNGT	6 cut(s)	57, 425, 921, 1024, 1033, 1935
Bst6I	CTCTTC	4 cut(s)	700, 1803, 1993, 2062
BstAPI	GCANNNNNTGC	2 cut(s)	992, 1439
BstC8I	GCNNGC	6 cut(s)	186, 337, 913, 1265, 1815, 1903
BstDSI	CCRYGG	1 cut(s)	222
BstENI	CCTNNNNNAGG	1 cut(s)	1466
BstF5I	GGATG	4 cut(s)	122, 410, 1508, 2042
BstFNI	CGCG	1 cut(s)	211
BstKTI	GATC	6 cut(s)	12, 1049, 1801, 1822, 1959, 2131
BstMAI	GTCTC	2 cut(s)	442, 1785
BstMBI	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
BstNI	CCWGG	5 cut(s)	27, 190, 384, 1388, 1562
BstSCI	CCNGG	6 cut(s)	25, 188, 382, 1386, 1560, 2105
BstSFI	CTRYAG	4 cut(s)	993, 1188, 1303, 1728
BstSLI	GKGCMC	2 cut(s)	148, 1619
BstUI	CGCG	1 cut(s)	211
BstV1I	GCAGC	8 cut(s)	167, 253, 571, 1077, 1254, 1429, 1889, 1892
BstX2I	RGATCY	3 cut(s)	9, 1046, 2128
BstYI	RGATCY	3 cut(s)	9, 1046, 2128
BstZ17I	GTATAC	1 cut(s)	1130
Bsu36I	CCTNAGG	1 cut(s)	1896
BsuRI	GGCC	4 cut(s)	252, 1560, 1726, 1780
BtgI	CCRYGG	1 cut(s)	222
BtsCI	GGATG	4 cut(s)	122, 410, 1508, 2042
BtsI	GCAGTG	2 cut(s)	319, 1992
BtsIMutI	CAGTG	6 cut(s)	32, 319, 679, 1937, 1959, 1992
BveI	ACCTGC	2 cut(s)	244, 1470
Cac8I	GCNNGC	6 cut(s)	186, 337, 913, 1265, 1815, 1903
CaiI	CAGNNNCTG	1 cut(s)	1939
CciI	TCATGA	1 cut(s)	2134
Cfr10I	RCCGGY	1 cut(s)	911
Cfr13I	GGNCC	3 cut(s)	251, 1382, 2063
Csp6I	GTAC	7 cut(s)	101, 443, 605, 1182, 1642, 1840, 1931
CviAII	CATG	8 cut(s)	92, 223, 706, 1379, 1538, 1612, 1648, 2135
CviQI	GTAC	7 cut(s)	101, 443, 605, 1182, 1642, 1840, 1931
DpnI	GATC	6 cut(s)	11, 1048, 1800, 1821, 1958, 2130
DpnII	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
DraI	TTTAAA	1 cut(s)	1504
DrdI	GACNNNNNNGTC	1 cut(s)	456
DseDI	GACNNNNNNGTC	1 cut(s)	456
Eam1104I	CTCTTC	4 cut(s)	700, 1803, 1993, 2062
EarI	CTCTTC	4 cut(s)	700, 1803, 1993, 2062
Eco130I	CCWWGG	1 cut(s)	222
Eco147I	AGGCCT	1 cut(s)	1726
Eco32I	GATATC	1 cut(s)	81
Eco47I	GGWCC	2 cut(s)	1382, 2063
Eco57I	CTGAAG	3 cut(s)	1599, 1961, 2145
Eco81I	CCTNAGG	1 cut(s)	1896
Eco88I	CYCGRG	1 cut(s)	1605
EcoNI	CCTNNNNNAGG	1 cut(s)	1466
EcoRII	CCWGG	5 cut(s)	25, 188, 382, 1386, 1560
EcoRV	GATATC	1 cut(s)	81
EcoT14I	CCWWGG	1 cut(s)	222
EcoT22I	ATGCAT	2 cut(s)	682, 962
ErhI	CCWWGG	1 cut(s)	222
Esp3I	CGTCTC	1 cut(s)	442
FaeI	CATG	8 cut(s)	95, 226, 709, 1382, 1541, 1615, 1651, 2138
FalI	AAGNNNNNCTT	4 cut(s)	133, 165, 1223, 1255
FaqI	GGGAC	2 cut(s)	1368, 1427
FatI	CATG	8 cut(s)	91, 222, 705, 1378, 1537, 1611, 1647, 2134
FauNDI	CATATG	1 cut(s)	1084
FblI	GTMKAC	1 cut(s)	1129
Fnu4HI	GCNGC	9 cut(s)	156, 209, 267, 560, 1066, 1268, 1443, 1878, 1881
FokI	GGATG	4 cut(s)	109, 397, 1495, 2029
Fsp4HI	GCNGC	9 cut(s)	156, 209, 267, 560, 1066, 1268, 1443, 1878, 1881
FspBI	CTAG	2 cut(s)	1664, 1824
GluI	GCNGC	9 cut(s)	156, 209, 267, 560, 1066, 1268, 1443, 1878, 1881
GsuI	CTGGAG	2 cut(s)	1544, 1620
HaeIII	GGCC	4 cut(s)	252, 1560, 1726, 1780
HapII	CCGG	3 cut(s)	912, 1653, 2107
Hin1II	CATG	8 cut(s)	95, 226, 709, 1382, 1541, 1615, 1651, 2138
HincII	GTYRAC	2 cut(s)	1027, 1983
HindII	GTYRAC	2 cut(s)	1027, 1983
HindIII	AAGCTT	3 cut(s)	488, 1289, 1360
HinfI	GANTC	7 cut(s)	889, 1118, 1316, 1472, 1759, 1973, 2030
HpaI	GTTAAC	1 cut(s)	1983
HpaII	CCGG	3 cut(s)	912, 1653, 2107
HphI	GGTGA	6 cut(s)	225, 752, 801, 828, 1238, 1934
Hpy166II	GTNNAC	5 cut(s)	146, 1027, 1130, 1681, 1983
Hpy188I	TCNGA	2 cut(s)	2035, 2125
Hpy188III	TCNNGA	8 cut(s)	542, 569, 1313, 1637, 1703, 1895, 1970, 2135
Hpy8I	GTNNAC	5 cut(s)	146, 1027, 1130, 1681, 1983
HpyAV	CCTTC	6 cut(s)	738, 795, 1574, 1709, 1716, 2132
HpyCH4III	ACNGT	6 cut(s)	57, 425, 921, 1024, 1033, 1935
Hsp92II	CATG	8 cut(s)	95, 226, 709, 1382, 1541, 1615, 1651, 2138
KroI	GCCGGC	1 cut(s)	911
KroNI	GCCGGC	1 cut(s)	913
KspAI	GTTAAC	1 cut(s)	1983
Kzo9I	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
LguI	GCTCTTC	1 cut(s)	1803
LmnI	GCTCC	7 cut(s)	223, 244, 358, 799, 1639, 1832, 1897
Lsp1109I	GCAGC	8 cut(s)	167, 253, 571, 1077, 1254, 1429, 1889, 1892
LweI	GCATC	8 cut(s)	131, 667, 689, 995, 1245, 1279, 1429, 1517
MaeI	CTAG	2 cut(s)	1664, 1824
MaeIII	GTNAC	7 cut(s)	30, 1226, 1298, 1313, 1737, 1765, 1935
MalI	GATC	6 cut(s)	11, 1048, 1800, 1821, 1958, 2130
MboI	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
MfeI	CAATTG	1 cut(s)	290
MflI	RGATCY	3 cut(s)	9, 1046, 2128
MhlI	GDGCHC	3 cut(s)	148, 220, 1619
MlyI	GAGTC	3 cut(s)	1310, 1466, 2039
MmeI	TCCRAC	1 cut(s)	444
Mph1103I	ATGCAT	2 cut(s)	682, 962
MroNI	GCCGGC	1 cut(s)	911
MroXI	GAANNNNTTC	1 cut(s)	2130
MslI	CAYNNNNRTG	2 cut(s)	96, 1421
MspA1I	CMGCKG	1 cut(s)	1187
MspI	CCGG	3 cut(s)	912, 1653, 2107
MspR9I	CCNGG	6 cut(s)	27, 190, 384, 1388, 1562, 2107
MunI	CAATTG	1 cut(s)	290
MvaI	CCWGG	5 cut(s)	27, 190, 384, 1388, 1562
MvnI	CGCG	1 cut(s)	211
NaeI	GCCGGC	1 cut(s)	913
NciI	CCSGG	1 cut(s)	2107
NcoI	CCATGG	1 cut(s)	222
NdeI	CATATG	1 cut(s)	1084
NdeII	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
NgoMIV	GCCGGC	1 cut(s)	911
NlaIII	CATG	8 cut(s)	95, 226, 709, 1382, 1541, 1615, 1651, 2138
NlaIV	GGNNCC	4 cut(s)	253, 910, 1384, 1575
NmuCI	GTSAC	6 cut(s)	30, 1226, 1313, 1737, 1765, 1935
NsiI	ATGCAT	2 cut(s)	682, 962
PagI	TCATGA	1 cut(s)	2134
PaqCI	CACCTGC	1 cut(s)	244
PasI	CCCWGGG	1 cut(s)	1387
PceI	AGGCCT	1 cut(s)	1726
PciSI	GCTCTTC	1 cut(s)	1803
PdiI	GCCGGC	1 cut(s)	913
PdmI	GAANNNNTTC	1 cut(s)	2130
PfeI	GAWTC	4 cut(s)	889, 1118, 1759, 1973
PflMI	CCANNNNNTGG	1 cut(s)	2072
PfoI	TCCNGGA	1 cut(s)	2105
PkrI	GCNGC	9 cut(s)	157, 210, 268, 561, 1067, 1269, 1444, 1879, 1882
PleI	GAGTC	3 cut(s)	1310, 1466, 2038
PpsI	GAGTC	3 cut(s)	1310, 1466, 2038
Psp6I	CCWGG	5 cut(s)	25, 188, 382, 1386, 1560
PspGI	CCWGG	5 cut(s)	25, 188, 382, 1386, 1560
PspN4I	GGNNCC	4 cut(s)	253, 910, 1384, 1575
PspPI	GGNCC	3 cut(s)	251, 1382, 2063
PstI	CTGCAG	1 cut(s)	997
PstNI	CAGNNNCTG	1 cut(s)	1939
PsuI	RGATCY	3 cut(s)	9, 1046, 2128
PvuII	CAGCTG	1 cut(s)	1187
RsaI	GTAC	7 cut(s)	102, 444, 606, 1183, 1643, 1841, 1932
RsaNI	GTAC	7 cut(s)	101, 443, 605, 1182, 1642, 1840, 1931
RseI	CAYNNNNRTG	2 cut(s)	96, 1421
SapI	GCTCTTC	1 cut(s)	1803
SatI	GCNGC	9 cut(s)	156, 209, 267, 560, 1066, 1268, 1443, 1878, 1881
Sau3AI	GATC	6 cut(s)	9, 1046, 1798, 1819, 1956, 2128
Sau96I	GGNCC	3 cut(s)	251, 1382, 2063
SchI	GAGTC	3 cut(s)	1310, 1466, 2039
ScrFI	CCNGG	6 cut(s)	27, 190, 384, 1388, 1562, 2107
SduI	GDGCHC	3 cut(s)	148, 220, 1619
SfaNI	GCATC	8 cut(s)	131, 667, 689, 995, 1245, 1279, 1429, 1517
SfcI	CTRYAG	4 cut(s)	993, 1188, 1303, 1728
SinI	GGWCC	2 cut(s)	1382, 2063
SmiMI	CAYNNNNRTG	2 cut(s)	96, 1421
SmlI	CTYRAG	1 cut(s)	789
SmoI	CTYRAG	1 cut(s)	789
SpeI	ACTAGT	1 cut(s)	1663
SseBI	AGGCCT	1 cut(s)	1726
SsiI	CCGC	1 cut(s)	209
SspI	AATATT	1 cut(s)	628
SspMI	CTAG	2 cut(s)	1664, 1824
StuI	AGGCCT	1 cut(s)	1726
StyD4I	CCNGG	6 cut(s)	25, 188, 382, 1386, 1560, 2105
StyI	CCWWGG	1 cut(s)	222
TaaI	ACNGT	6 cut(s)	57, 425, 921, 1024, 1033, 1935
TaqI	TCGA	1 cut(s)	362
TatI	WGTACW	1 cut(s)	604
TauI	GCSGC	1 cut(s)	211
TfiI	GAWTC	4 cut(s)	889, 1118, 1759, 1973
TscAI	CASTG	6 cut(s)	39, 319, 679, 1944, 1966, 1999
TseFI	GTSAC	6 cut(s)	30, 1226, 1313, 1737, 1765, 1935
TseI	GCWGC	8 cut(s)	155, 266, 559, 1065, 1267, 1442, 1877, 1880
Tsp45I	GTSAC	6 cut(s)	30, 1226, 1313, 1737, 1765, 1935
TspDTI	ATGAA	8 cut(s)	65, 720, 881, 1101, 1367, 1439, 1526, 2123
TspGWI	ACGGA	1 cut(s)	956
TspRI	CASTG	6 cut(s)	39, 319, 679, 1944, 1966, 1999
Van91I	CCANNNNNTGG	1 cut(s)	2072
VneI	GTGCAC	1 cut(s)	144
VpaK11BI	GGWCC	2 cut(s)	1382, 2063
XagI	CCTNNNNNAGG	1 cut(s)	1466
XcmI	CCANNNNNNNNNTGG	2 cut(s)	811, 1519
XmiI	GTMKAC	1 cut(s)	1129
XmnI	GAANNNNTTC	1 cut(s)	2130
XspI	CTAG	2 cut(s)	1664, 1824
Zsp2I	ATGCAT	2 cut(s)	682, 962

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.