AT4G11110

spa1-related 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012794
Physical Location & Seq
Forward (+)
6771605 .. 6777225
5621 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G11110.1

Sequence Viewer

Length: 3111 bp
ATGATGGATGAGGGATCAGTAGGGGATGTGAGTAGGATTGATGAAGCTGATGTTGCTCACCTTCAATTCAAGAATAGTGAGCAGTCGTTTAAACCGGAGAACATTGAAGTCCGGGAAGTAAAAGAAGTGCAGGTTCAGAGAGAAGCTGGTTCTCCTGATTGTTCTTATGGTGTAATAGCAGATTTTTTAGATGGTAAGAATGGTGGTGACCATGTGGAGCTTATAGGGAATGAACCATGTTCTAGCCGGCAGAATACAAATGATGAGGGGGATGTTGTCGAGGAGTTGACTGTTAAAACTTGTGAGGGATCGAGTATGGCTATAGTTGGTAGACCAAGTAGTAGAGCTAGATTAGAGATGAATCGCAGTCAGTTTCTGCATCGTTTTCCGCTAGACGGTGATTTACCGGGTAGTAGTTCTATGAGTAAGAAGGTAATTGACAGGGGAACAGTAAGTATCTTGAGAAATGCTGGGAAAATGTCTTTGCCTGAGACTTCAAATGGACAACTGGCTATTATTGCTGTTAATGGAGAAGCTAATGAACATTTGACAAATGTTGAACGAAATCCTGTACCTGTAGAGGCATTGTCTCATGAAGGCATCAAGACAAAGATGTTATCGCAGTCTGGGTTTTCTCAGTTTTTTGTTAGAAAAACATTGAAAGGGAAGGGAGTTACTTTCAGAGGCCCGCCAAACAATAGATCTAAAGCGAGGAATATGGATCAGCAAACTGTAGCTAGTTCTGGTAGCGCTTTAGTAATAGCTAATACTTCAGCAAAGATTTCTAGCAGCATCCCTTTGGCGGCTTATGATGGTTTGCCGTGCTTACCTTCCAATACCAGTAAACCATCTTCTTGTGCTAATCCTTCAGATACACATAGAGGTTGTGGAGGTGAAGGTTTAAGTCTGAGAGAATGGTTGAAGTCGGAGCGGCAGGAAGTTAATAAAGCAGAATGTATGTATATCTTTAGGCAGATCGTGGATCATGTGGACTGTTCTCATTCTCAAGGAGTTGTTTTATGTGACCTGCGACCATCTTCATTCAAAATTTTCAAGGAAAATGCAGTAAAGTATGTGGTTTCAGGTTCTCAACGAGAGTCTTTTGACAGCAACATGAACAAAGAGACTTTAAGTCAACTCGAAAACCCTCTGGTTAGAAGACGGTTAGGAGATACTTCTAGTCTCTCTATCCCTGCAAAGAAGCAGAAATCAAGTGGACCAAGCTCTAGACAGTGGCCTATGTTTCAACGAGCTGGTGGCGTCAATATCCAAACTGAAAATAATGATGGCGCGATACAGGAGTTTCATTTCAGATCTAGTCAACCTCATTGTAGTACAGTTGCCTGCCCTTTTACTTCAGTGAGTGAGCAGTTGGAAGAGAAATGGTATGCGAGCCCTGAGGAGCTCAGGGGTGACATGCGTTCAGCCTCTTCAAACATCTATAGTTTGGGTATTCTTCTTTATGAGTTGCTGAGCCAATTTCAGTGTGAAAGAGCTCGTGAGGCTGCAATGTCTGATATCCGACACCGCATTCTTCCTCCCAAATTTCTGTCGGAGAATCCAAAGGAAGCTGGGTTTTGTCTTTGGTTACTTCATCCAGAATCTTCATGCCGACCATCAACCAGGGACATCTTACAATCTGAAGTAGTTAATGGAATCCCGGATTTGTATGCTGAAGGCTTATCTTTATCCATTGAACAAGAGGACACAGAGTCTGAACTCTTGCAGCATTTCCTTTTCTTGTCCCAAGAGAAAAGGCAGAAGCATGCTGGAAATTTGATGGAAGAAATTGCGTCCGTAGAAGCAGATATCGAGGAAATTGTCAAGAGACGTTGTGCCATAGGGCCTCCGTCCCTGGAAGAAGCTTCTAGCTCATCTCCTGCTTCTAGTGTACCTGAAATGAGACTGATCAGGAACATTAATCAGCTTGAAAGCGCTTATTTCGCTGCAAGGATAGACGCACATCTCCCTGAAGCACGTTATAGGTTACGCCCAGATAGAGATCTGCTAAGAAATAGTGATAATACTGTAGCAGAAGTAGAAAACAGTGAGACTTGGAGCTCAGATGATCGTGTTGGAGCGTTCTTTGATGGGTTATGCAAATATGCTCGATATAGTAAGTTTGAAACTCGGGGTGTGCTGAGGACCAGTGAGTTGAACAACACTTCAAATGTGATTTGTTCTCTGGGTTTTGATCGGGACGAGGATTATTTTGCCACAGCTGGTGTGTCGAAAAAAATTAAGATATATGAGTTCAATTCCCTTTTCAACGAATCTGTTGACATTCATTATCCAGCCATAGAAATGCCAAATAGATCAAAGCTTAGCGGCGTTTGCTGGAACAACTACATTAGGAACTACCTAGCTTCCTCTGATTATGATGGTATTGTCAAGTTATGGGATGTGACAACAGGACAGGCCATTTCACATTTCATAGAGCATGAGAAGAGGGCTTGGTCTGTTGACTTCTCTGAAGCTTGTCCGACAAAATTAGCCAGTGGAAGCGATGATTGTTCTGTGAAGCTATGGAACATCAATGAGAGGAACTGCTTAGGAACAATCAGGAACATTGCAAACGTTTGTTGTGTGCAATTTTCTCCACAGTCCTCTCACTTGCTAGCCTTTGGATCATCAGACTTCAGAACCTACTGCTATGATCTAAGGAATTTAAGAACTCCTTGGTGCATACTGTCAGGACACAACAAAGCAGTTAGCTATGCGAAGTTCTTGGATAATGAAACTCTTGTAACAGCATCAACTGATAACACGTTAAAGCTTTGGGATCTTAAGAAAACCACTCACGGTGGCCTCTCAACAAATGCTTGCAGCTTAACTTTTGGCGGCCATACAAATGAGAAGAACTTTGTTGGTTTGTCTACTTCTGATGGATATATAGCTTGTGGCTCTGAGACAAATGAGGTTTATGCCTATCACCGGTCTCTTCCCATGCCAATTACCTCGTACAAGTTTGGTTCTATTGATCCAATATCAGGTAAGGAGATAGAGGAGGACAACAACCTGTTCGTTTCAAGCGTTTGCTGGAGAAAAAGATCGAATATGGTTGTTTCAGCTAGCTCAAATGGGTCAATCAAAGTTCTACAGTTGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

1036

Amino Acids

115.0

Weight (kDa)

5.76

Isoelectric Point (pI)

54.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 456 - 548 8.2e-06 Protein kinase domain
Beta-prop_TEP1_2nd PF25047 715 - 844 8.8e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 719 - 978 1.3e-34 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 724 - 845 5.5e-10 WDR3 first beta-propeller domain
Beta-prop_RIG_2nd PF23775 728 - 820 8.9e-07 RIG second beta-propeller
WD40_Gbeta PF25391 731 - 889 2.9e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 763 - 927 2.7e-13 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 772 - 927 2.5e-18 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 774 - 972 2.4e-20 THOC3 beta-propeller domain
WD40_Prp19 PF24814 788 - 909 2.7e-12 Prp19 WD40 domain
EIF3I PF24805 788 - 875 8e-08 EIF3I
WD40_WDHD1_1st PF24817 812 - 934 2.8e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 829 - 977 1.5e-17 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 830 - 978 1.2e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 830 - 941 1.4e-08 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 830 - 932 4.4e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 831 - 933 2.3e-09 TEP-1 second beta-propeller
WD40_Prp19 PF24814 858 - 971 2.8e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 873 - 1034 6.2e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 121, 1035
AccBSI CCGCTC 1 cut(s) 931
AccI GTMKAC 2 cut(s) 331, 2876
AccII CGCG 1 cut(s) 1292
AciI CCGC 7 cut(s) 389, 689, 803, 931, 1528, 2328, 2841
AclI AACGTT 1 cut(s) 2577
AclWI GGATC 7 cut(s) 22, 316, 729, 990, 2635, 2790, 2975
AcoI YGGCCR 1 cut(s) 2842
AcsI RAATTY 4 cut(s) 1047, 1544, 1774, 2665
AcuI CTGAAG 8 cut(s) 756, 852, 1341, 1662, 1695, 1992, 2493, 2623
AcyI GRCGYC 1 cut(s) 1260
AfaI GTAC 4 cut(s) 573, 1336, 1893, 2963
AfeI AGCGCT 2 cut(s) 751, 1936
AfiI CCNNNNNNNGG 4 cut(s) 395, 802, 2934, 2990
AflII CTTAAG 1 cut(s) 2786
AflIII ACRYGT 1 cut(s) 2766
AgeI ACCGGT 1 cut(s) 2934
AhdI GACNNNNNGTC 2 cut(s) 1131, 1711
AjnI CCWGG 2 cut(s) 1622, 1854
Alw21I GWGCWC 3 cut(s) 1407, 1498, 2063
Alw26I GTCTC 9 cut(s) 485, 594, 1118, 1187, 1822, 1897, 2045, 2903, 2943
AlwI GGATC 7 cut(s) 22, 316, 729, 990, 2635, 2790, 2975
AlwNI CAGNNNCTG 2 cut(s) 376, 1715
Ama87I CYCGRG 1 cut(s) 2130
Aor51HI AGCGCT 2 cut(s) 751, 1936
AoxI GGCC 6 cut(s) 685, 1235, 1844, 2418, 2806, 2842
ApeKI GCWGC 5 cut(s) 789, 1505, 1726, 1946, 2826
ApoI RAATTY 4 cut(s) 1047, 1544, 1774, 2665
AseI ATTAAT 1 cut(s) 1920
AsiGI ACCGGT 1 cut(s) 2934
AspLEI GCGC 3 cut(s) 752, 1292, 1937
AspS9I GGNCC 4 cut(s) 686, 1217, 1844, 2145
AsuC2I CCSGG 3 cut(s) 113, 408, 1661
AsuHPI GGTGA 6 cut(s) 50, 218, 410, 905, 1424, 2924
AsuNHI GCTAGC 2 cut(s) 2617, 3071
AvaI CYCGRG 1 cut(s) 2130
AvaII GGWCC 2 cut(s) 1217, 2145
AxyI CCTNAGG 1 cut(s) 1398
BaeI ACNNNNGTAYC 2 cut(s) 1286, 1319
BanII GRGCYC 4 cut(s) 1397, 1407, 1498, 2063
BarI GAAGNNNNNNTAC 2 cut(s) 835, 867
BauI CACGAG 1 cut(s) 1497
BbsI GAAGAC 1 cut(s) 1165
Bbv12I GWGCWC 3 cut(s) 1407, 1498, 2063
BbvCI CCTCAGC 1 cut(s) 2141
BbvI GCAGC 5 cut(s) 801, 1492, 1738, 1933, 2838
BceAI ACGGC 1 cut(s) 805
BciT130I CCWGG 2 cut(s) 1624, 1856
BclI TGATCA 1 cut(s) 1908
BcnI CCSGG 3 cut(s) 113, 408, 1661
BcoDI GTCTC 9 cut(s) 485, 594, 1118, 1187, 1822, 1897, 2045, 2903, 2943
BfmI CTRYAG 6 cut(s) 321, 576, 732, 1441, 2028, 3098
BfoI RGCGCY 2 cut(s) 753, 1938
BfrI CTTAAG 1 cut(s) 2786
BfuAI ACCTGC 2 cut(s) 121, 1035
BglII AGATCT 3 cut(s) 701, 1313, 2002
BisI GCNGC 9 cut(s) 790, 804, 932, 1506, 1727, 1947, 2329, 2827, 2842
BlpI GCTNAGC 2 cut(s) 1472, 2324
BlsI GCNGC 9 cut(s) 791, 805, 933, 1507, 1728, 1948, 2330, 2828, 2843
Bme1390I CCNGG 5 cut(s) 113, 408, 1624, 1661, 1856
Bme18I GGWCC 2 cut(s) 1217, 2145
BmeRI GACNNNNNGTC 2 cut(s) 1131, 1711
BmeT110I CYCGRG 1 cut(s) 2130
BmgT120I GGNCC 4 cut(s) 686, 1217, 1844, 2145
BmrFI CCNGG 5 cut(s) 113, 408, 1624, 1661, 1856
BmsI GCATC 4 cut(s) 388, 609, 801, 2762
BmtI GCTAGC 2 cut(s) 2621, 3075
BpiI GAAGAC 1 cut(s) 1165
BpmI CTGGAG 1 cut(s) 3061
Bpu10I CCTNAGC 3 cut(s) 1406, 2141, 2551
Bpu1102I GCTNAGC 2 cut(s) 1472, 2324
BpuEI CTTGAG 2 cut(s) 481, 990
BpuMI CCSGG 3 cut(s) 113, 408, 1661
BsaBI GATNNNNATC 1 cut(s) 2001
BsaHI GRCGYC 1 cut(s) 1260
BsaI GGTCTC 1 cut(s) 2943
BsaJI CCNNGG 3 cut(s) 1623, 1854, 2678
BsaWI WCCGGW 2 cut(s) 94, 2934
Bsc4I CCNNNNNNNGG 4 cut(s) 395, 802, 2934, 2990
Bse118I RCCGGY 2 cut(s) 246, 2934
Bse1I ACTGG 4 cut(s) 513, 840, 2148, 2496
Bse21I CCTNAGG 1 cut(s) 1398
Bse3DI GCAATG 2 cut(s) 1515, 2568
Bse8I GATNNNNATC 1 cut(s) 2001
BseBI CCWGG 2 cut(s) 1624, 1856
BseDI CCNNGG 3 cut(s) 1623, 1854, 2678
BseGI GGATG 6 cut(s) 13, 31, 277, 792, 1594, 2407
BseJI GATNNNNATC 1 cut(s) 2001
BseLI CCNNNNNNNGG 4 cut(s) 395, 802, 2934, 2990
BseMI GCAATG 2 cut(s) 1515, 2568
BseMII CTCAG 9 cut(s) 480, 650, 899, 1389, 1420, 1463, 2076, 2132, 2898
BseNI ACTGG 4 cut(s) 513, 840, 2148, 2496
BseRI GAGGAG 3 cut(s) 296, 1415, 3020
BseXI GCAGC 5 cut(s) 801, 1492, 1738, 1933, 2838
BseYI CCCAGC 2 cut(s) 470, 1571
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 1292
BshFI GGCC 6 cut(s) 687, 1237, 1846, 2420, 2808, 2844
BshTI ACCGGT 1 cut(s) 2934
BsiHKAI GWGCWC 3 cut(s) 1407, 1498, 2063
BsiHKCI CYCGRG 1 cut(s) 2130
BsiSI CCGG 6 cut(s) 95, 112, 247, 407, 1661, 2935
BslFI GGGAC 4 cut(s) 1640, 1729, 1837, 2213
BslI CCNNNNNNNGG 4 cut(s) 395, 802, 2934, 2990
BsmAI GTCTC 9 cut(s) 485, 594, 1118, 1187, 1822, 1897, 2045, 2903, 2943
BsmBI CGTCTC 1 cut(s) 1822
BsmFI GGGAC 4 cut(s) 1640, 1729, 1837, 2213
BsmI GAATGC 1 cut(s) 1530
BsnI GGCC 6 cut(s) 687, 1237, 1846, 2420, 2808, 2844
Bso31I GGTCTC 1 cut(s) 2943
BsoBI CYCGRG 1 cut(s) 2130
Bsp1286I GDGCHC 4 cut(s) 1397, 1407, 1498, 2063
Bsp1720I GCTNAGC 2 cut(s) 1472, 2324
BspACI CCGC 7 cut(s) 389, 689, 803, 931, 1528, 2328, 2841
BspANI GGCC 6 cut(s) 687, 1237, 1846, 2420, 2808, 2844
BspCNI CTCAG 9 cut(s) 481, 649, 900, 1390, 1419, 1464, 2075, 2133, 2899
BspFNI CGCG 1 cut(s) 1292
BspHI TCATGA 1 cut(s) 592
BspMI ACCTGC 2 cut(s) 121, 1035
BspOI GCTAGC 2 cut(s) 2621, 3075
BspPI GGATC 7 cut(s) 22, 316, 729, 990, 2635, 2790, 2975
BspTI CTTAAG 1 cut(s) 2786
BspTNI GGTCTC 1 cut(s) 2943
BsrBI CCGCTC 1 cut(s) 931
BsrDI GCAATG 2 cut(s) 1515, 2568
BsrFI RCCGGY 2 cut(s) 246, 2934
BsrI ACTGG 4 cut(s) 513, 840, 2148, 2496
BssAI RCCGGY 2 cut(s) 246, 2934
BssECI CCNNGG 3 cut(s) 1623, 1854, 2678
BssNI GRCGYC 1 cut(s) 1260
BssSI CACGAG 1 cut(s) 1497
BssT1I CCWWGG 1 cut(s) 2678
Bst2BI CACGAG 1 cut(s) 1497
Bst2UI CCWGG 2 cut(s) 1624, 1856
Bst6I CTCTTC 4 cut(s) 1371, 1435, 2441, 2946
BstACI GRCGYC 1 cut(s) 1260
BstAFI CTTAAG 1 cut(s) 2786
BstC8I GCNNGC 8 cut(s) 248, 689, 1345, 1393, 1767, 2619, 2824, 3073
BstEII GGTNACC 1 cut(s) 206
BstF5I GGATG 6 cut(s) 13, 31, 277, 792, 1594, 2407
BstFNI CGCG 1 cut(s) 1292
BstH2I RGCGCY 2 cut(s) 753, 1938
BstHHI GCGC 3 cut(s) 752, 1292, 1937
BstMAI GTCTC 9 cut(s) 485, 594, 1118, 1187, 1822, 1897, 2045, 2903, 2943
BstMWI GCNNNNNNNGC 5 cut(s) 53, 518, 1502, 1943, 2334
BstNI CCWGG 2 cut(s) 1624, 1856
BstNSI RCATGY 2 cut(s) 1420, 1769
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 5 cut(s) 111, 406, 1622, 1659, 1854
BstSFI CTRYAG 6 cut(s) 321, 576, 732, 1441, 2028, 3098
BstUI CGCG 1 cut(s) 1292
BstV1I GCAGC 5 cut(s) 801, 1492, 1738, 1933, 2838
BstV2I GAAGAC 1 cut(s) 1165
BstX2I RGATCY 4 cut(s) 701, 1313, 2002, 2782
BstYI RGATCY 4 cut(s) 701, 1313, 2002, 2782
Bsu36I CCTNAGG 1 cut(s) 1398
BsuRI GGCC 6 cut(s) 687, 1237, 1846, 2420, 2808, 2844
BtgZI GCGATG 1 cut(s) 2520
BtsCI GGATG 6 cut(s) 13, 31, 277, 792, 1594, 2407
BtsIMutI CAGTG 6 cut(s) 1238, 1365, 1490, 2053, 2155, 2503
BveI ACCTGC 2 cut(s) 121, 1035
Cac8I GCNNGC 8 cut(s) 248, 689, 1345, 1393, 1767, 2619, 2824, 3073
CaiI CAGNNNCTG 2 cut(s) 376, 1715
CciI TCATGA 1 cut(s) 592
CfoI GCGC 3 cut(s) 752, 1292, 1937
Cfr10I RCCGGY 2 cut(s) 246, 2934
Cfr13I GGNCC 4 cut(s) 686, 1217, 1844, 2145
CseI GACGC 3 cut(s) 1249, 1782, 1967
Csp6I GTAC 4 cut(s) 572, 1335, 1892, 2962
CspAI ACCGGT 1 cut(s) 2934
CviQI GTAC 4 cut(s) 572, 1335, 1892, 2962
DraI TTTAAA 1 cut(s) 91
DriI GACNNNNNGTC 2 cut(s) 1131, 1711
EaeI YGGCCR 1 cut(s) 2842
Eam1104I CTCTTC 4 cut(s) 1371, 1435, 2441, 2946
Eam1105I GACNNNNNGTC 2 cut(s) 1131, 1711
EarI CTCTTC 4 cut(s) 1371, 1435, 2441, 2946
Ecl136II GAGCTC 3 cut(s) 1405, 1496, 2061
Eco130I CCWWGG 1 cut(s) 2678
Eco24I GRGCYC 4 cut(s) 1397, 1407, 1498, 2063
Eco31I GGTCTC 1 cut(s) 2943
Eco32I GATATC 2 cut(s) 1519, 1810
Eco47I GGWCC 2 cut(s) 1217, 2145
Eco47III AGCGCT 2 cut(s) 751, 1936
Eco53kI GAGCTC 3 cut(s) 1405, 1496, 2061
Eco57I CTGAAG 8 cut(s) 756, 852, 1341, 1662, 1695, 1992, 2493, 2623
Eco81I CCTNAGG 1 cut(s) 1398
Eco88I CYCGRG 1 cut(s) 2130
Eco91I GGTNACC 1 cut(s) 206
EcoICRI GAGCTC 3 cut(s) 1405, 1496, 2061
EcoO109I RGGNCCY 1 cut(s) 1844
EcoO65I GGTNACC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 1622, 1854
EcoRV GATATC 2 cut(s) 1519, 1810
EcoT14I CCWWGG 1 cut(s) 2678
EcoT38I GRGCYC 4 cut(s) 1397, 1407, 1498, 2063
ErhI CCWWGG 1 cut(s) 2678
Esp3I CGTCTC 1 cut(s) 1822
FalI AAGNNNNNCTT 2 cut(s) 2662, 2694
FaqI GGGAC 4 cut(s) 1640, 1729, 1837, 2213
FauI CCCGC 1 cut(s) 696
FbaI TGATCA 1 cut(s) 1908
FblI GTMKAC 2 cut(s) 331, 2876
Fnu4HI GCNGC 9 cut(s) 790, 804, 932, 1506, 1727, 1947, 2329, 2827, 2842
FokI GGATG 6 cut(s) 20, 38, 284, 779, 1581, 2414
FriOI GRGCYC 4 cut(s) 1397, 1407, 1498, 2063
Fsp4HI GCNGC 9 cut(s) 790, 804, 932, 1506, 1727, 1947, 2329, 2827, 2842
GlaI GCGC 3 cut(s) 751, 1291, 1936
GluI GCNGC 9 cut(s) 790, 804, 932, 1506, 1727, 1947, 2329, 2827, 2842
GsaI CCCAGC 2 cut(s) 474, 1575
GsuI CTGGAG 1 cut(s) 3061
HaeII RGCGCY 2 cut(s) 753, 1938
HaeIII GGCC 6 cut(s) 687, 1237, 1846, 2420, 2808, 2844
HapII CCGG 6 cut(s) 95, 112, 247, 407, 1661, 2935
HgaI GACGC 3 cut(s) 1249, 1782, 1967
HhaI GCGC 3 cut(s) 752, 1292, 1937
Hin1I GRCGYC 1 cut(s) 1260
Hin6I GCGC 3 cut(s) 750, 1290, 1935
HinP1I GCGC 3 cut(s) 750, 1290, 1935
HincII GTYRAC 5 cut(s) 288, 1136, 1322, 2281, 2464
HindII GTYRAC 5 cut(s) 288, 1136, 1322, 2281, 2464
HindIII AAGCTT 4 cut(s) 1863, 2321, 2475, 2774
HinfI GANTC 7 cut(s) 361, 1097, 1558, 1601, 1656, 1712, 2273
HpaII CCGG 6 cut(s) 95, 112, 247, 407, 1661, 2935
HphI GGTGA 6 cut(s) 50, 218, 410, 905, 1424, 2924
HpyAV CCTTC 8 cut(s) 71, 424, 590, 661, 840, 876, 890, 1670
HpyCH4IV ACGT 4 cut(s) 1831, 1978, 2577, 2768
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 518, 1502, 1943, 2334
HpySE526I ACGT 4 cut(s) 1831, 1978, 2577, 2768
Hsp92I GRCGYC 1 cut(s) 1260
HspAI GCGC 3 cut(s) 750, 1290, 1935
KroI GCCGGC 1 cut(s) 246
KroNI GCCGGC 1 cut(s) 248
Ksp22I TGATCA 1 cut(s) 1908
LmnI GCTCC 5 cut(s) 217, 928, 1402, 2058, 2078
Lsp1109I GCAGC 5 cut(s) 801, 1492, 1738, 1933, 2838
LweI GCATC 4 cut(s) 388, 609, 801, 2762
MaeII ACGT 4 cut(s) 1831, 1978, 2577, 2768
MaeIII GTNAC 8 cut(s) 206, 673, 1022, 1412, 1587, 1986, 2404, 2746
MbiI CCGCTC 1 cut(s) 931
MflI RGATCY 4 cut(s) 701, 1313, 2002, 2782
MhlI GDGCHC 4 cut(s) 1397, 1407, 1498, 2063
MlyI GAGTC 2 cut(s) 1106, 1721
MmeI TCCRAC 6 cut(s) 906, 1353, 1533, 1546, 2056, 2507
MroNI GCCGGC 1 cut(s) 246
MslI CAYNNNNRTG 2 cut(s) 2303, 2850
MspA1I CMGCKG 1 cut(s) 2222
MspCI CTTAAG 1 cut(s) 2786
MspI CCGG 6 cut(s) 95, 112, 247, 407, 1661, 2935
MspR9I CCNGG 5 cut(s) 113, 408, 1624, 1661, 1856
MssI GTTTAAAC 1 cut(s) 91
Mva1269I GAATGC 1 cut(s) 1530
MvaI CCWGG 2 cut(s) 1624, 1856
MvnI CGCG 1 cut(s) 1292
MwoI GCNNNNNNNGC 5 cut(s) 53, 518, 1502, 1943, 2334
NaeI GCCGGC 1 cut(s) 248
NciI CCSGG 3 cut(s) 113, 408, 1661
NgoMIV GCCGGC 1 cut(s) 246
NheI GCTAGC 2 cut(s) 2617, 3071
NmuCI GTSAC 4 cut(s) 206, 1022, 1412, 2404
NspI RCATGY 2 cut(s) 1420, 1769
PaeI GCATGC 1 cut(s) 1769
PagI TCATGA 1 cut(s) 592
PcsI WCGNNNNNNNCGW 1 cut(s) 3030
PctI GAATGC 1 cut(s) 1530
PdiI GCCGGC 1 cut(s) 248
PfeI GAWTC 5 cut(s) 361, 1558, 1601, 1656, 2273
PfoI TCCNGGA 2 cut(s) 111, 1659
PinAI ACCGGT 1 cut(s) 2934
PkrI GCNGC 9 cut(s) 791, 805, 933, 1507, 1728, 1948, 2330, 2828, 2843
PleI GAGTC 2 cut(s) 1105, 1720
PmeI GTTTAAAC 1 cut(s) 91
PpsI GAGTC 2 cut(s) 1105, 1720
PshBI ATTAAT 1 cut(s) 1920
Psp124BI GAGCTC 3 cut(s) 1407, 1498, 2063
Psp1406I AACGTT 1 cut(s) 2577
Psp6I CCWGG 2 cut(s) 1622, 1854
PspEI GGTNACC 1 cut(s) 206
PspFI CCCAGC 2 cut(s) 470, 1571
PspGI CCWGG 2 cut(s) 1622, 1854
PspPI GGNCC 4 cut(s) 686, 1217, 1844, 2145
PstNI CAGNNNCTG 2 cut(s) 376, 1715
PsuI RGATCY 4 cut(s) 701, 1313, 2002, 2782
PvuII CAGCTG 1 cut(s) 2222
RsaI GTAC 4 cut(s) 573, 1336, 1893, 2963
RsaNI GTAC 4 cut(s) 572, 1335, 1892, 2962
RseI CAYNNNNRTG 2 cut(s) 2303, 2850
SacI GAGCTC 3 cut(s) 1407, 1498, 2063
SatI GCNGC 9 cut(s) 790, 804, 932, 1506, 1727, 1947, 2329, 2827, 2842
Sau96I GGNCC 4 cut(s) 686, 1217, 1844, 2145
SchI GAGTC 2 cut(s) 1106, 1721
ScrFI CCNGG 5 cut(s) 113, 408, 1624, 1661, 1856
SduI GDGCHC 4 cut(s) 1397, 1407, 1498, 2063
SfaNI GCATC 4 cut(s) 388, 609, 801, 2762
SfcI CTRYAG 6 cut(s) 321, 576, 732, 1441, 2028, 3098
SinI GGWCC 2 cut(s) 1217, 2145
SmiMI CAYNNNNRTG 2 cut(s) 2303, 2850
SmlI CTYRAG 3 cut(s) 460, 1005, 2786
SmoI CTYRAG 3 cut(s) 460, 1005, 2786
SphI GCATGC 1 cut(s) 1769
SsiI CCGC 7 cut(s) 389, 689, 803, 931, 1528, 2328, 2841
SstI GAGCTC 3 cut(s) 1407, 1498, 2063
StyD4I CCNGG 5 cut(s) 111, 406, 1622, 1659, 1854
StyI CCWWGG 1 cut(s) 2678
TaiI ACGT 4 cut(s) 1834, 1981, 2580, 2771
TaqI TCGA 7 cut(s) 279, 311, 1140, 1812, 2110, 2231, 3053
TatI WGTACW 1 cut(s) 1334
TauI GCSGC 4 cut(s) 806, 934, 2331, 2844
TfiI GAWTC 5 cut(s) 361, 1558, 1601, 1656, 2273
TscAI CASTG 6 cut(s) 1238, 1365, 1490, 2053, 2155, 2503
TseFI GTSAC 4 cut(s) 206, 1022, 1412, 2404
TseI GCWGC 5 cut(s) 789, 1505, 1726, 1946, 2826
Tsp45I GTSAC 4 cut(s) 206, 1022, 1412, 2404
TspGWI ACGGA 2 cut(s) 1786, 1839
TspRI CASTG 6 cut(s) 1238, 1365, 1490, 2053, 2155, 2503
Vha464I CTTAAG 1 cut(s) 2786
VpaK11BI GGWCC 2 cut(s) 1217, 2145
VspI ATTAAT 1 cut(s) 1920
XapI RAATTY 4 cut(s) 1047, 1544, 1774, 2665
XbaI TCTAGA 1 cut(s) 1226
XceI RCATGY 2 cut(s) 1420, 1769
XmiI GTMKAC 2 cut(s) 331, 2876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.