MD14G1121100.v1.1

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012794
Physical Location & Seq
Reverse (-)
19552966 .. 19561253
8288 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1121100.v1.1.491

Sequence Viewer

Length: 3201 bp
ATGGAGGATGCAGTGGATGAGGAAGTGACCCCATTAGATTTAGCAGAGGTTGTACAACGTGAGAGGAAAGAGAATGAGCACTCAGTTAAATCTGAAAACGGCAACCCATTGGAATGCCAAGAAATGTGTACATATGGTGAATGTGATTATTCGATGCGTATACCAGTTGAGGGTGATTATCCCTTAAGCTCGCGGCATGAGTTTGTAGAGAATGTAGATATGGCTATGAGCCCTGTTAACGAATTAGAACACCCATATGGTAGCCTTGGCTCTATGAATGATGTTGGTTCCATGGAGGAAGAGCCAACTGTGAGAAATTACAACAATCAAGGAAGAATGCAATTTTGGCAGAACCCACAACAACATTTATTTCAGCTAGCAAGTGGATCAGGAAGTGAGAGTTCACAAGTCAATACTGCATTCAAGGATAATGGGAAGGCAATTTCTGGTGGCTTGGAGAATGAGGGGTCCACATCTGTCTCCGGTTTTTGGAATCAAAGGGCATTAAGTGACAATCACTATGATGTTGTGGAAGAATTAACAAACATTGAAAATACAGGGGCTTCAGGCAATACTTATGCAGGTATCCGAACAAAAATTCTATCTAGGCCAGGCTTTTCCGAGTTCTTTGTTAAGAATACACTGAAGGGTAAGGGTGTCATAATGAAGGGTCTGAACCATGAGAATTGTCACATTGAATCTAGAAACCTGAATAGTTCAAAGGTGGCCGGTGATCCTTTGGCAGCTTCTGATCCAACAATGAGTATGGATGCCAATGTTATTATGCCTTCTTCCAATGGTGTTAATGCTGGACGTACGGCTGGTGGCTCTTATCATGATGAAAACAGTTTGAGAGAATGGCTGAATACTGGACGTCCTAAAGCGAATAAAGCTGAATGCTTGTATATATTTAGACAGATAGTGGATCTGGTGGATAATTTTCATTCTAAAGGAGTTGCTTTGCCTGGATTGCGACCTTCTTTCTTCAAGTTGTTACCTTCAAATCAGGTTAAGTATGTTGGCTTACCAGTCCCAAGCGAGATGCTTGAGAGTACGAAGAAGAGAGATATATCTCTTTTAGAAAATAGTCCAGTAAGGAAAAAGCAAAGGGTCAGTGAGAATACTAGATTGCAATGGCCCCAGTTCTCTACCACTTCCTACTTCATGCATGATAATGTGAATAACAGCCACATCAACATCACTGGTTTGCAGAACAAAAGTGATGCATTTGGTGAACAACATCCAAGAATAAGGCATGAGATACGTACCATGTTCACCAGTCCTCATATGCATTATGCAACTCAGCAGTTTACTTCAATAAATGAGCTATTGGAAGAGAAGTGGTATATTAGTCCTGAGGAACTCAGTGAAGGAAGTTACACAGTTTTATCAAATGTCTATGATCTTGGTGTTCTTCTTTTCGAGTTACTTGCTCATTTTGACTCAAATAGCGCCCTTGCTGCAGCAATGTCCAACCTGTGCTACAGAATTCTTCCCCCAAAATTTCTGTCAGAAAATGCTATGGAGGCTGGATTTATTATTTGGCTTCTTCATCCTGAACCATCTTCACGCCCAACGACAAGGCAAATTCTACAATCTGAAGTACTTAACGGGTTACAGGAGGTTTGTGTAAAAGAATTGTCACCTTCTGTGGAACAAGAGGACGCAGAATTGGATTTATTATTGCATTTCCTCACTTCTTTGAAAGAACAGATGCAGAACCATGATGCAACTTTGTTGGAAACAGTTCAGTTCTTAGAAGCAGATGTTGAAGAAGTGGAGAGGAGGCACTCTGCAAGAAAACCCTTGATTGATTGTGGCTTGTACGGTGAATCCTTAAGGAAAAATATGTTCATTAGCAAAGAAGATTCAAGGTCAGAGGTGCTTTCTCCATTATTTCCTGTTCTTAGTTCAAATGAGTCGAGGTTGATGAAAAATATAGATCAGTTTGAGAGTGCTTACTTCTTGATGAGATCAAGAATTCAGATTCCTGAGACGGATTCTAGAATACGCAGCGATAAAGATTTATTAAGAACTCGCAATAATTGGTATGTGGGAACAAAGGATGAAGACAAGGAGATATGCACTGATCGACCGGGAGCCATCTTTGATGGTTTGTGTAAATATGCTCGGTATAGTAAGTTTGAAGTACGTGGTATACTAAGAAATGGAGATTTCAGCAGTTCCTCTAAAGTAATTTCCTCTATGAGTTTTGATCGGGATGAAGACTATTTTGCTACTGCCGGGGTTTCAATGAAAATCAAGATTTTTGAGTTTAATGCTTTCTTCAACAATCCTGCTGATATTCATTATCCAGTGATTGAGATGTCAAACAAATCAAGGATCAGCTGTGTTTGCTGGAACAACTATATAAAAAACTATCTGGCTTCATCTGATTATGATGGTGTAATCAAGAGAAAGGATCCTCGCCGGATCCTCTTTGTGAAGATCCCGGGGATCCTCCAATCACATTCGTTCATCGTACATCATGCGTCCAGTTTTGGTCAGTTATGGGATGCCAGCACTGGTCAAGAGTTTGCTCAATACACTGAGCATGCAATGAGGGCTTGGTCTGTAGACTTTTCTCAAGTATATCCTACAAAATTAGCCACTGGGAGTGATGATGGTTGTAGAAACAGTTTAGATACCATCAAGCACGCTGCAAATGTATGCTGTGTACAGTTCTCTTCTCACTCCTCTCATTTGCTGGCCTTCGGGAGTGCGGACTACTACACCTACTGCTATGATCTCCGTAATACTAAAATTCCGTGGTGCGTATTGGATGGGCATAAGAAAGCTGTAAGCTATGTGAAATTTTTGGACTCAGAGACCCTTGTTTCTGCATCCACTGACAACACATTAAAGCTATGGGATCTTAGCAAAACCAGTATGAGTGATACATCCACCGATGCTTGCAGCCTAAGCCTTGGTGGGCACACCAATGAGAAGAACTTTGTCGGGTTATCCGTTTCAGATGATTACATAGCTTGTGGTTCAGAAACAAATGAGGTTTATGCTTACCATAGATCTCTGCCTATGCCGATCACTTCACACAAGTTTGGCTCCATTGATCCTATTTCTGGAAAGGAGACCAATGATTATAGTGCACAATGGGTTTCGAGTGTCTGCTGGAGAGGGAAATCAGACATGGTGGTTGCTGCCAATTCAGACGGGTGTATAAAAGTGCAGAAGTTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

1067

Amino Acids

120.06

Weight (kDa)

5.49

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 440 - 534 1e-05 Protein kinase domain
Beta-prop_WDR5 PF25175 836 - 1008 3.2e-13 WDR5 beta-propeller domain
Beta-prop_EIPR1 PF23609 881 - 957 4e-07 EIPR1 beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 881 - 957 5.8e-08 WDR36/Utp21 first beta-propeller
WDR55 PF24796 882 - 962 4.7e-06 WDR55
Beta-prop_TEP1_2nd PF25047 883 - 962 9.6e-09 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 884 - 968 2.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 885 - 1007 2.5e-13 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 885 - 983 3.2e-08 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 886 - 1065 3.1e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 887 - 1001 8.8e-09 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 877
Acc36I ACCTGC 1 cut(s) 572
AccI GTMKAC 3 cut(s) 160, 2158, 2577
AccII CGCG 1 cut(s) 193
AciI CCGC 2 cut(s) 193, 2723
AcoI YGGCCR 1 cut(s) 726
AcsI RAATTY 7 cut(s) 597, 1488, 1502, 1587, 1980, 2763, 2813
AcuI CTGAAG 3 cut(s) 549, 665, 1620
AcyI GRCGYC 1 cut(s) 874
AfiI CCNNNNNNNGG 3 cut(s) 170, 489, 3080
AflII CTTAAG 2 cut(s) 184, 1837
AjnI CCWGG 2 cut(s) 610, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
Alw21I GWGCWC 2 cut(s) 81, 3109
Alw26I GTCTC 4 cut(s) 484, 1988, 2822, 3083
Alw44I GTGCAC 1 cut(s) 3105
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 2452
AoxI GGCC 4 cut(s) 608, 726, 1136, 2709
ApaLI GTGCAC 1 cut(s) 3105
ApeKI GCWGC 7 cut(s) 743, 1460, 1463, 2013, 2660, 2916, 3158
ApoI RAATTY 7 cut(s) 597, 1488, 1502, 1587, 1980, 2763, 2813
ArsI GACNNNNNNTTYG 2 cut(s) 1493, 1525
Asp700I GAANNNNTTC 1 cut(s) 1746
AspLEI GCGC 1 cut(s) 1454
AspS9I GGNCC 2 cut(s) 468, 1137
AsuC2I CCSGG 4 cut(s) 2097, 2245, 2453, 2454
AsuHPI GGTGA 7 cut(s) 149, 185, 743, 1244, 1267, 1635, 1841
AsuNHI GCTAGC 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 2452
AvaII GGWCC 1 cut(s) 468
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 2 cut(s) 2937, 3109
BamHI GGATCC 3 cut(s) 2422, 2433, 2457
BanII GRGCYC 1 cut(s) 233
BbsI GAAGAC 2 cut(s) 2076, 2232
Bbv12I GWGCWC 2 cut(s) 81, 3109
BbvI GCAGC 7 cut(s) 755, 1447, 1475, 2025, 2647, 2928, 3145
BccI CCATC 7 cut(s) 1570, 2105, 2111, 2396, 2618, 2657, 2777
BceAI ACGGC 2 cut(s) 115, 834
BcgI CGANNNNNNTGC 3 cut(s) 1412, 1446, 3176
BciT130I CCWGG 2 cut(s) 612, 966
BciVI GTATCC 1 cut(s) 596
BcnI CCSGG 4 cut(s) 2097, 2245, 2453, 2454
BcoDI GTCTC 4 cut(s) 484, 1988, 2822, 3083
BfaI CTAG 5 cut(s) 377, 606, 702, 1125, 2004
BfmI CTRYAG 3 cut(s) 1461, 1483, 2574
BfoI RGCGCY 1 cut(s) 1455
BfrI CTTAAG 2 cut(s) 184, 1837
BfuAI ACCTGC 1 cut(s) 572
BfuI GTATCC 1 cut(s) 596
BglII AGATCT 1 cut(s) 3026
BisI GCNGC 8 cut(s) 194, 744, 1461, 1464, 2014, 2661, 2917, 3159
BlsI GCNGC 8 cut(s) 195, 745, 1462, 1465, 2015, 2662, 2918, 3160
BmcAI AGTACT 1 cut(s) 1605
Bme1390I CCNGG 6 cut(s) 612, 966, 2097, 2245, 2453, 2454
Bme18I GGWCC 1 cut(s) 468
BmeT110I CYCGRG 1 cut(s) 2452
BmgT120I GGNCC 2 cut(s) 468, 1137
BmiI GGNNCC 8 cut(s) 289, 469, 1139, 2101, 2424, 2435, 2459, 3064
BmrFI CCNGG 6 cut(s) 612, 966, 2097, 2245, 2453, 2454
BmrI ACTGGG 2 cut(s) 1135, 2622
BmsI GCATC 9 cut(s) 144, 760, 1032, 1213, 1704, 1717, 2506, 2852, 2899
BmtI GCTAGC 1 cut(s) 380
BmuI ACTGGG 2 cut(s) 1135, 2622
BpiI GAAGAC 2 cut(s) 2076, 2232
BpmI CTGGAG 1 cut(s) 3151
Bpu10I CCTNAGC 1 cut(s) 2921
BpuEI CTTGAG 2 cut(s) 1067, 2571
BpuMI CCSGG 4 cut(s) 2097, 2245, 2453, 2454
BsaAI YACGTR 2 cut(s) 1265, 2153
BsaHI GRCGYC 1 cut(s) 874
BsaI GGTCTC 2 cut(s) 2822, 3083
BsaJI CCNNGG 7 cut(s) 265, 291, 2244, 2452, 2453, 2768, 2926
BsaWI WCCGGW 1 cut(s) 482
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 489, 3080
Bse118I RCCGGY 1 cut(s) 728
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 3 cut(s) 1139, 1473, 2565
BseBI CCWGG 2 cut(s) 612, 966
BseDI CCNNGG 7 cut(s) 265, 291, 2244, 2452, 2453, 2768, 2926
BseLI CCNNNNNNNGG 3 cut(s) 170, 489, 3080
BseMI GCAATG 3 cut(s) 1139, 1473, 2565
BseMII CTCAG 7 cut(s) 96, 1316, 1347, 1378, 1983, 2541, 2838
BseRI GAGGAG 2 cut(s) 1798, 2686
BseSI GKGCMC 2 cut(s) 2937, 3109
BseXI GCAGC 7 cut(s) 755, 1447, 1475, 2025, 2647, 2928, 3145
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 2096
BshFI GGCC 4 cut(s) 610, 728, 1138, 2711
BsiEI CGRYCG 1 cut(s) 2096
BsiHKAI GWGCWC 2 cut(s) 81, 3109
BsiHKCI CYCGRG 1 cut(s) 2452
BsiSI CCGG 6 cut(s) 483, 729, 2096, 2244, 2431, 2453
BsiWI CGTACG 1 cut(s) 815
BslFI GGGAC 1 cut(s) 1016
BslI CCNNNNNNNGG 3 cut(s) 170, 489, 3080
BsmAI GTCTC 4 cut(s) 484, 1988, 2822, 3083
BsmBI CGTCTC 1 cut(s) 1988
BsmFI GGGAC 1 cut(s) 1016
BsmI GAATGC 4 cut(s) 119, 342, 419, 902
BsnI GGCC 4 cut(s) 610, 728, 1138, 2711
Bso31I GGTCTC 2 cut(s) 2822, 3083
BsoBI CYCGRG 1 cut(s) 2452
Bsp1286I GDGCHC 4 cut(s) 81, 233, 2937, 3109
Bsp1407I TGTACA 3 cut(s) 52, 128, 2677
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 2 cut(s) 193, 2723
BspANI GGCC 4 cut(s) 610, 728, 1138, 2711
BspCNI CTCAG 7 cut(s) 95, 1315, 1348, 1377, 1984, 2542, 2837
BspFNI CGCG 1 cut(s) 193
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 8 cut(s) 289, 469, 1139, 2101, 2424, 2435, 2459, 3064
BspMAI CTGCAG 1 cut(s) 1465
BspMI ACCTGC 1 cut(s) 572
BspOI GCTAGC 1 cut(s) 380
BspQI GCTCTTC 1 cut(s) 294
BspTI CTTAAG 2 cut(s) 184, 1837
BspTNI GGTCTC 2 cut(s) 2822, 3083
BsrDI GCAATG 3 cut(s) 1139, 1473, 2565
BsrFI RCCGGY 1 cut(s) 728
BsrGI TGTACA 3 cut(s) 52, 128, 2677
BssAI RCCGGY 1 cut(s) 728
BssECI CCNNGG 7 cut(s) 265, 291, 2244, 2452, 2453, 2768, 2926
BssNAI GTATAC 2 cut(s) 161, 2159
BssNI GRCGYC 1 cut(s) 874
BssT1I CCWWGG 3 cut(s) 265, 291, 2926
Bst1107I GTATAC 2 cut(s) 161, 2159
Bst2UI CCWGG 2 cut(s) 612, 966
Bst4CI ACNGT 7 cut(s) 310, 848, 1384, 1747, 1829, 2639, 2682
Bst6I CTCTTC 4 cut(s) 294, 1055, 1329, 2692
BstACI GRCGYC 1 cut(s) 874
BstAFI CTTAAG 2 cut(s) 184, 1837
BstAUI TGTACA 3 cut(s) 52, 128, 2677
BstBAI YACGTR 2 cut(s) 1265, 2153
BstC8I GCNNGC 7 cut(s) 191, 378, 2521, 2556, 2658, 2709, 2914
BstDSI CCRYGG 2 cut(s) 291, 2768
BstFNI CGCG 1 cut(s) 193
BstH2I RGCGCY 1 cut(s) 1455
BstHHI GCGC 1 cut(s) 1454
BstMAI GTCTC 4 cut(s) 484, 1988, 2822, 3083
BstMCI CGRYCG 1 cut(s) 2096
BstMWI GCNNNNNNNGC 8 cut(s) 346, 890, 970, 1460, 1526, 2355, 2564, 2922
BstNI CCWGG 2 cut(s) 612, 966
BstNSI RCATGY 1 cut(s) 2558
BstSCI CCNGG 6 cut(s) 610, 964, 2095, 2243, 2451, 2452
BstSFI CTRYAG 3 cut(s) 1461, 1483, 2574
BstSLI GKGCMC 2 cut(s) 2937, 3109
BstSNI TACGTA 1 cut(s) 1265
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 7 cut(s) 755, 1447, 1475, 2025, 2647, 2928, 3145
BstV2I GAAGAC 2 cut(s) 2076, 2232
BstX2I RGATCY 7 cut(s) 925, 2422, 2433, 2448, 2457, 2872, 3026
BstYI RGATCY 7 cut(s) 925, 2422, 2433, 2448, 2457, 2872, 3026
BstZ17I GTATAC 2 cut(s) 161, 2159
Bsu36I CCTNAGG 1 cut(s) 1356
BsuI GTATCC 1 cut(s) 596
BsuRI GGCC 4 cut(s) 610, 728, 1138, 2711
BtgI CCRYGG 2 cut(s) 291, 2768
BtsI GCAGTG 1 cut(s) 18
BveI ACCTGC 1 cut(s) 572
Cac8I GCNNGC 7 cut(s) 191, 378, 2521, 2556, 2658, 2709, 2914
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 835
CfoI GCGC 1 cut(s) 1454
Cfr10I RCCGGY 1 cut(s) 728
Cfr13I GGNCC 2 cut(s) 468, 1137
Cfr9I CCCGGG 1 cut(s) 2452
CseI GACGC 2 cut(s) 1673, 2481
EaeI YGGCCR 1 cut(s) 726
Eam1104I CTCTTC 4 cut(s) 294, 1055, 1329, 2692
EarI CTCTTC 4 cut(s) 294, 1055, 1329, 2692
Eco105I TACGTA 1 cut(s) 1265
Eco130I CCWWGG 3 cut(s) 265, 291, 2926
Eco24I GRGCYC 1 cut(s) 233
Eco31I GGTCTC 2 cut(s) 2822, 3083
Eco47I GGWCC 1 cut(s) 468
Eco57I CTGAAG 3 cut(s) 549, 665, 1620
Eco81I CCTNAGG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 2452
EcoRI GAATTC 2 cut(s) 1488, 1980
EcoRII CCWGG 2 cut(s) 610, 964
EcoT14I CCWWGG 3 cut(s) 265, 291, 2926
EcoT22I ATGCAT 3 cut(s) 1170, 1228, 1293
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 3 cut(s) 265, 291, 2926
Esp3I CGTCTC 1 cut(s) 1988
FaqI GGGAC 1 cut(s) 1016
FauNDI CATATG 3 cut(s) 133, 256, 1287
FblI GTMKAC 3 cut(s) 160, 2158, 2577
Fnu4HI GCNGC 8 cut(s) 194, 744, 1461, 1464, 2014, 2661, 2917, 3159
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 8 cut(s) 194, 744, 1461, 1464, 2014, 2661, 2917, 3159
FspBI CTAG 5 cut(s) 377, 606, 702, 1125, 2004
GlaI GCGC 1 cut(s) 1453
GluI GCNGC 8 cut(s) 194, 744, 1461, 1464, 2014, 2661, 2917, 3159
GsuI CTGGAG 1 cut(s) 3151
HaeII RGCGCY 1 cut(s) 1455
HaeIII GGCC 4 cut(s) 610, 728, 1138, 2711
HapII CCGG 6 cut(s) 483, 729, 2096, 2244, 2431, 2453
HgaI GACGC 2 cut(s) 1673, 2481
HhaI GCGC 1 cut(s) 1454
Hin1I GRCGYC 1 cut(s) 874
Hin6I GCGC 1 cut(s) 1452
HinP1I GCGC 1 cut(s) 1452
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 9 cut(s) 493, 698, 1442, 1832, 1868, 1919, 1987, 2000, 2822
HpaI GTTAAC 1 cut(s) 238
HpaII CCGG 6 cut(s) 483, 729, 2096, 2244, 2431, 2453
HphI GGTGA 7 cut(s) 149, 185, 743, 1244, 1267, 1635, 1841
HpyAV CCTTC 9 cut(s) 430, 640, 661, 798, 987, 1008, 1364, 1656, 2722
HpyCH4III ACNGT 7 cut(s) 310, 848, 1384, 1747, 1829, 2639, 2682
HpyCH4IV ACGT 5 cut(s) 58, 814, 874, 1264, 2152
HpyF10VI GCNNNNNNNGC 8 cut(s) 346, 890, 970, 1460, 1526, 2355, 2564, 2922
HpySE526I ACGT 5 cut(s) 58, 814, 874, 1264, 2152
Hsp92I GRCGYC 1 cut(s) 874
HspAI GCGC 1 cut(s) 1452
KspAI GTTAAC 1 cut(s) 238
LguI GCTCTTC 1 cut(s) 294
LmnI GCTCC 2 cut(s) 2099, 3068
Lsp1109I GCAGC 7 cut(s) 755, 1447, 1475, 2025, 2647, 2928, 3145
LweI GCATC 9 cut(s) 144, 760, 1032, 1213, 1704, 1717, 2506, 2852, 2899
MaeI CTAG 5 cut(s) 377, 606, 702, 1125, 2004
MaeII ACGT 5 cut(s) 58, 814, 874, 1264, 2152
MaeIII GTNAC 8 cut(s) 25, 509, 689, 993, 1376, 1425, 1614, 1641
MflI RGATCY 7 cut(s) 925, 2422, 2433, 2448, 2457, 2872, 3026
MhlI GDGCHC 4 cut(s) 81, 233, 2937, 3109
MlyI GAGTC 3 cut(s) 1436, 1928, 2816
MmeI TCCRAC 3 cut(s) 779, 1497, 1719
Mph1103I ATGCAT 3 cut(s) 1170, 1228, 1293
MroXI GAANNNNTTC 1 cut(s) 1746
MslI CAYNNNNRTG 5 cut(s) 112, 255, 522, 1173, 2940
MspA1I CMGCKG 1 cut(s) 2349
MspCI CTTAAG 2 cut(s) 184, 1837
MspI CCGG 6 cut(s) 483, 729, 2096, 2244, 2431, 2453
MspR9I CCNGG 6 cut(s) 612, 966, 2097, 2245, 2453, 2454
Mva1269I GAATGC 4 cut(s) 119, 342, 419, 902
MvaI CCWGG 2 cut(s) 612, 966
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 8 cut(s) 346, 890, 970, 1460, 1526, 2355, 2564, 2922
NciI CCSGG 4 cut(s) 2097, 2245, 2453, 2454
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 3 cut(s) 133, 256, 1287
NheI GCTAGC 1 cut(s) 376
NlaIV GGNNCC 8 cut(s) 289, 469, 1139, 2101, 2424, 2435, 2459, 3064
NmuCI GTSAC 4 cut(s) 25, 509, 689, 1641
NsiI ATGCAT 3 cut(s) 1170, 1228, 1293
NspI RCATGY 1 cut(s) 2558
PaeI GCATGC 1 cut(s) 2558
PagI TCATGA 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 2964
PctI GAATGC 4 cut(s) 119, 342, 419, 902
PdmI GAANNNNTTC 1 cut(s) 1746
PfeI GAWTC 6 cut(s) 493, 698, 1832, 1868, 1987, 2000
Pfl23II CGTACG 1 cut(s) 815
PkrI GCNGC 8 cut(s) 195, 745, 1462, 1465, 2015, 2662, 2918, 3160
PleI GAGTC 3 cut(s) 1436, 1927, 2816
PpsI GAGTC 3 cut(s) 1436, 1927, 2816
Ppu21I YACGTR 2 cut(s) 1265, 2153
Psp6I CCWGG 2 cut(s) 610, 964
PspGI CCWGG 2 cut(s) 610, 964
PspLI CGTACG 1 cut(s) 815
PspN4I GGNNCC 8 cut(s) 289, 469, 1139, 2101, 2424, 2435, 2459, 3064
PspPI GGNCC 2 cut(s) 468, 1137
PstI CTGCAG 1 cut(s) 1465
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 7 cut(s) 925, 2422, 2433, 2448, 2457, 2872, 3026
PvuII CAGCTG 1 cut(s) 2349
RseI CAYNNNNRTG 5 cut(s) 112, 255, 522, 1173, 2940
SapI GCTCTTC 1 cut(s) 294
SatI GCNGC 8 cut(s) 194, 744, 1461, 1464, 2014, 2661, 2917, 3159
Sau96I GGNCC 2 cut(s) 468, 1137
ScaI AGTACT 1 cut(s) 1605
SchI GAGTC 3 cut(s) 1436, 1928, 2816
ScrFI CCNGG 6 cut(s) 612, 966, 2097, 2245, 2453, 2454
SduI GDGCHC 4 cut(s) 81, 233, 2937, 3109
SfaNI GCATC 9 cut(s) 144, 760, 1032, 1213, 1704, 1717, 2506, 2852, 2899
SfcI CTRYAG 3 cut(s) 1461, 1483, 2574
SinI GGWCC 1 cut(s) 468
SmaI CCCGGG 1 cut(s) 2454
SmiMI CAYNNNNRTG 5 cut(s) 112, 255, 522, 1173, 2940
SmlI CTYRAG 4 cut(s) 184, 1046, 1837, 2586
SmoI CTYRAG 4 cut(s) 184, 1046, 1837, 2586
SnaBI TACGTA 1 cut(s) 1265
SphI GCATGC 1 cut(s) 2558
SsiI CCGC 2 cut(s) 193, 2723
SspMI CTAG 5 cut(s) 377, 606, 702, 1125, 2004
StyD4I CCNGG 6 cut(s) 610, 964, 2095, 2243, 2451, 2452
StyI CCWWGG 3 cut(s) 265, 291, 2926
TaaI ACNGT 7 cut(s) 310, 848, 1384, 1747, 1829, 2639, 2682
TaiI ACGT 5 cut(s) 61, 817, 877, 1267, 2155
TaqI TCGA 5 cut(s) 152, 1422, 1922, 2092, 3119
TatI WGTACW 4 cut(s) 52, 128, 1603, 2677
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 6 cut(s) 493, 698, 1832, 1868, 1987, 2000
TseFI GTSAC 4 cut(s) 25, 509, 689, 1641
TseI GCWGC 7 cut(s) 743, 1460, 1463, 2013, 2660, 2916, 3158
Tsp45I GTSAC 4 cut(s) 25, 509, 689, 1641
TspGWI ACGGA 4 cut(s) 2012, 2741, 2757, 2956
TspMI CCCGGG 1 cut(s) 2452
Vha464I CTTAAG 2 cut(s) 184, 1837
VneI GTGCAC 1 cut(s) 3105
VpaK11BI GGWCC 1 cut(s) 468
XapI RAATTY 7 cut(s) 597, 1488, 1502, 1587, 1980, 2763, 2813
XbaI TCTAGA 2 cut(s) 701, 2003
XceI RCATGY 1 cut(s) 2558
XmaI CCCGGG 1 cut(s) 2452
XmiI GTMKAC 3 cut(s) 160, 2158, 2577
XmnI GAANNNNTTC 1 cut(s) 1746
XspI CTAG 5 cut(s) 377, 606, 702, 1125, 2004
ZraI GACGTC 1 cut(s) 875
ZrmI AGTACT 1 cut(s) 1605
Zsp2I ATGCAT 3 cut(s) 1170, 1228, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.