FvH4_5g04010

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012794
Physical Location & Seq
Reverse (-)
2381602 .. 2388087
6486 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04010.t1

Sequence Viewer

Length: 2997 bp
ATGGAGAATGCAGTGGGTAGGGAGGTGGCCGTGCAGGATCAGACGGAGGGGGTGCAGCTTCAGAGTAAGGAGAATGAGCATGCGTTGAGACTGGAAAATGGCCACATGTTTGACTGCAGGGAGATGCGTGCACCGGGTGAAGAAGATCATTCATTGCGTATACCGGTTGGAAGCAGTTGTCCTGAGAGCTCGAGTCAGGAGTGTTTTGTGAACCCGCATGATGGTTTAGGCTTGTATCCGAGTGGATCAAGGAGCGGGAGTGGTGGGAGTTCACGAGTCAATACTGCATTTATAGATTTTGGTCAGGTAAGATCAAATGATTTGGAGAATAAGGAGTTCCCATCTTTGGATGATATTATGGTTCAAAAGGCAAATAGTGACAATCTGGTTGAAGTTGTGGCAGAATTGACAAGTACTCCAGATGCAGGGGTTTCAGGCAATGCATGTGGAGGTATCCGGACAAAAATGCTTTCTAAATCAAGATCAGAATATTATGTTAAGAATACATTGAAGGGTAAGGGTGTTATTTTCAAAGGTCCAAATCATGGTGGCGGTCCCGTTCAATCTAAGAACCTCAACAGTACAAAGGTTACCGGTGCTTCTAGGGCTACTTCTGATCCAGCACAGAGTTTGGATGTCAGAATTGTTATGCCTATTTCCACTGATGTGAACGTTGTGCCCAGACCGCCAGCCTTGGAAAATGATGAAATCAGTTTAAGAGAATGGCTGAGAATTGAACGTCCTAAAGTGAATAAAGTTGTGTGCCTGTATGTATTCAAACAAATTGCAAATATCGTGAATCAGTTTCACTCTCAAGGTGTTGCTTTGCAAGAATTGCACCCTTCTTTTTTTAAGATGTTGCCTTCAAATGAGGTCAAGTATGTTGGCTTACCAATCCCAAAAGAGATACTAGAAAGTTCTATGGATAAAGGTATCTGTCATTCAGAAAATAGTTCAAAGAAAATAATGTCAGTGGAGCATGAATTTGTTTCTGTTGGTTCAAGTGCAAAGAAGCAAAAGATCAGCCAGAGCACTAGATTGGTTAAGCAATGGCCTCAGTTCCCCACTTCTTCCTGTTTCCAGCGTGAAACTAGGAATGCTAGCCACTTCAACATTAATGGTCTCCGAAATAGATCTCCTATAATGTATAATGCAGCTCCACAACACTTGACTTCAATCACCGAACAGTTGGAAGAAAAGTGGTATATCAGTCCTGAAGAACTCAATGAAGGAAGCTGTACTGTTTTGTCAAATATCTACAGTCTGGGCGTTCTTCTTTTTGAGTTGCTTGCGCAATTTGACTCAAATAGTGCCATTGTCACAGCAATGTTCAATCTTCGTCATAGAATTCTTCCTCCAAGTTTTCTGTCAGAATACGCCAAGGAGGCTGGATTTTGTCTTTGGTTACTTCATCCTGAACCGTCTTCACGTCCAACAACAAGGGAAATTCTACAATCGGAAGTAGTTAATGGAATACAGGAGGTGTGTGAAGAGAAAGTGGCATCTTCTGTGGTCGAAGATGATGCTGAATTGGAACTATTATCTCATTTCCTCATGTTTATGAAAGAAAAGAAGCAGAAGGATGCCTTCAAGTTGATGGAAAGCATCAGGGTTTTAGAAGCAGATGTTGAAGAAGCTGAGAGGAGGTACTGTATAAGAAAACCCTTGATTGATCCCGACTGGTACAAGGAGTCCCTGGGTGGATGGAAAAATGCATGTAAAGAAGCTTCAAGATCGGAGATGCTTCCTCTTTTACCCCCTCTTCCCAGTTCAAATGAGTCAAGGTTGACGAGGAATATTGATCAGCTTGAGAGTGCTTACTTCTCTATGAGAAGGAAGATTCAGCCTACTGAAGCTGATTCTACTATACGTATAGATAAAGAGTTATTAAAAACTCGCAAAAATTGGTATATGAAATCAGAGGATGATGAAAATAAGACTCCTACGGATCAATTGGGAGCTATCTTTGATGGTTTATGCAAGTATGATCGGTACAGTAAGTTTGAAGTATGTGGAGTACTAAGAAATGGAGATCTCAACAGTAACTCAAATTTGATTTGCTCTTTGAGTTTTGATCGGGATGAGGACTACATCGCTTCTGCCGGGGTTTCAAAGAAAATAAAGATTTATGAGTTTAATGCACTGTTCAATGATTCTGTTGATATTCATTATCCATCGATCGAGATGTCAAATGAATCAAAGATTAGCTGTATTTGCTGGAACAACTACATAAAAAACTATCTCACTTCAGCTGATCATGAAGGTTTAGTGAAGTTGTGGGATGTGAGCACTGGTCAAGAGTTTGCTCGATACAGAGGGCATGAACAAAAAGCTTGGTCTGTTGACTTTTCTCAAGTAGATCCTACAAAATTAGCCAGTGGAAGTGATGATGGTACTGTGAAACTATGGAGCATTAATGAGAAAAACTGTACAGGCACCATCAGAAACAGAAACATTGCAAATGTATGCTGTGTACAGTTCTCTGCTCACTCCACTAATTTGCTGGGCTTCGGGTCTGCGGATTACAACACATATTGCTATGATCTTCGTAATATTAGAACACCCTGGTGTGTATTGGTTGGTCACCAGAAAAGCGTAAGCTATGCCAAATTCTTGGACTCTGAGACCCTTGTTTCTGCATCCACCGACAACACGTTGAAGCTATGGGATCTCAACAAAACCACTGCAAGTGGTCCATCCACCAATGCTTGCAGCAATACCCTCAGTGGGCACACCAATGAGAAGAACTTTGTGGGCTTATCTGTTTCTGATGGTTATATAGCGTGTGGTTCAGAAACAAATGAGGTTTATGCTTATTATAGATCTCTGCCTATGCCAATCACTTCACACAAATTCGGCTCCATTGATCCTATAACCGGAAAGGAGACGGATGAAATTAATGGACCATTTGTTTCGAGCATCTGCTGGAGACAGAAATCAAAAATGGTGGTTGCCGCTAATTCAAGCGGGTGCATAAAAGTGTTGCAGATTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

999

Amino Acids

111.52

Weight (kDa)

5.88

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 393 - 487 5.4e-06 Protein kinase domain
Beta-prop_WDR5 PF25175 680 - 939 9.6e-33 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 681 - 848 8.1e-16 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 682 - 806 1.4e-06 TEP-1 second beta-propeller
WDR55 PF24796 684 - 815 1.5e-07 WDR55
WD40_Gbeta PF25391 685 - 851 4.1e-08 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 686 - 895 2.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 688 - 809 1.9e-06 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 688 - 775 3.3e-07 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 697 - 889 5e-12 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 728 - 814 4.6e-08 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 729 - 841 1.9e-14 THOC3 beta-propeller domain
WD40_Prp19 PF24814 730 - 817 1.7e-08 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 746 - 894 4.8e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 748 - 895 2.6e-10 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 748 - 835 8.1e-07 EIF3I
WD40 PF00400 769 - 804 2.6e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 778 - 896 5.8e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 790 - 939 1.1e-18 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 791 - 894 1.7e-10 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 792 - 937 6.8e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 820 - 932 3e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 821 - 996 9.3e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1293
AccB1I GGYRCC 1 cut(s) 2435
AccB7I CCANNNNNTGG 1 cut(s) 545
AccBSI CCGCTC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 160
AccIII TCCGGA 1 cut(s) 456
AciI CCGC 7 cut(s) 215, 255, 552, 686, 2519, 2955, 2967
AclI AACGTT 1 cut(s) 672
AclWI GGATC 8 cut(s) 45, 253, 611, 1667, 1956, 2354, 2676, 2861
AcoI YGGCCR 2 cut(s) 27, 100
AcsI RAATTY 6 cut(s) 983, 1347, 1446, 2050, 2609, 2852
AcuI CTGAAG 4 cut(s) 44, 1236, 1872, 2232
AdeI CACNNNGTG 1 cut(s) 137
AfiI CCNNNNNNNGG 6 cut(s) 221, 346, 425, 545, 2727, 2876
AflIII ACRYGT 2 cut(s) 105, 2652
AgeI ACCGGT 2 cut(s) 163, 593
AjiI CACGTC 1 cut(s) 1430
AjnI CCWGG 2 cut(s) 1695, 2564
AleI CACNNNNGTG 2 cut(s) 665, 2566
Alw21I GWGCWC 4 cut(s) 133, 191, 1034, 2291
Alw26I GTCTC 5 cut(s) 82, 1127, 2618, 2879, 2923
Alw44I GTGCAC 1 cut(s) 129
AlwI GGATC 8 cut(s) 45, 253, 611, 1667, 1956, 2354, 2676, 2861
Ama87I CYCGRG 1 cut(s) 190
Aor13HI TCCGGA 1 cut(s) 456
AoxI GGCC 3 cut(s) 27, 100, 1052
ApaLI GTGCAC 1 cut(s) 129
ApeKI GCWGC 3 cut(s) 55, 1154, 2712
ApoI RAATTY 6 cut(s) 983, 1347, 1446, 2050, 2609, 2852
AseI ATTAAT 3 cut(s) 1116, 2415, 2898
AsiGI ACCGGT 2 cut(s) 163, 593
AspLEI GCGC 1 cut(s) 1294
AspS9I GGNCC 4 cut(s) 536, 554, 2693, 2903
AsuC2I CCSGG 2 cut(s) 135, 2104
AsuHPI GGTGA 3 cut(s) 149, 1171, 2576
AsuNHI GCTAGC 1 cut(s) 1100
AvaI CYCGRG 1 cut(s) 190
AvaII GGWCC 4 cut(s) 536, 554, 2693, 2903
BaeGI GKGCMC 3 cut(s) 133, 681, 2733
BalI TGGCCA 1 cut(s) 102
BanI GGYRCC 1 cut(s) 2435
BanII GRGCYC 1 cut(s) 191
BarI GAAGNNNNNNTAC 2 cut(s) 1711, 1743
BauI CACGAG 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 1416
Bbv12I GWGCWC 4 cut(s) 133, 191, 1034, 2291
BbvI GCAGC 3 cut(s) 67, 1166, 2724
BceAI ACGGC 1 cut(s) 14
BcgI CGANNNNNNTGC 2 cut(s) 1505, 1539
BciT130I CCWGG 2 cut(s) 1697, 2566
BciVI GTATCC 2 cut(s) 246, 464
BclI TGATCA 2 cut(s) 1801, 2254
BcnI CCSGG 2 cut(s) 135, 2104
BcoDI GTCTC 5 cut(s) 82, 1127, 2618, 2879, 2923
BfaI CTAG 5 cut(s) 603, 911, 1035, 1092, 1101
BfmI CTRYAG 2 cut(s) 115, 1258
BfuI GTATCC 2 cut(s) 246, 464
BglI GCCNNNNNGGC 1 cut(s) 1385
BglII AGATCT 3 cut(s) 1133, 2032, 2822
BisI GCNGC 4 cut(s) 56, 1155, 2713, 2955
BlsI GCNGC 4 cut(s) 57, 1156, 2714, 2956
BmcAI AGTACT 2 cut(s) 415, 2019
Bme1390I CCNGG 4 cut(s) 135, 1697, 2104, 2566
Bme18I GGWCC 4 cut(s) 536, 554, 2693, 2903
BmeT110I CYCGRG 1 cut(s) 190
BmgBI CACGTC 1 cut(s) 1430
BmgT120I GGNCC 4 cut(s) 536, 554, 2693, 2903
BmiI GGNNCC 3 cut(s) 556, 2437, 2860
BmrFI CCNGG 4 cut(s) 135, 1697, 2104, 2566
BmrI ACTGGG 1 cut(s) 1761
BmsI GCATC 9 cut(s) 114, 412, 1511, 1513, 1573, 1614, 1731, 2648, 2928
BmtI GCTAGC 1 cut(s) 1104
BmuI ACTGGG 1 cut(s) 1761
BpiI GAAGAC 1 cut(s) 1416
BpmI CTGGAG 2 cut(s) 402, 2947
BpuEI CTTGAG 3 cut(s) 798, 1829, 2337
BpuMI CCSGG 2 cut(s) 135, 2104
Bsa29I ATCGAT 1 cut(s) 2177
BsaAI YACGTR 1 cut(s) 1871
BsaI GGTCTC 2 cut(s) 1127, 2618
BsaJI CCNNGG 6 cut(s) 693, 1380, 1695, 1696, 2103, 2564
BsaWI WCCGGW 4 cut(s) 163, 456, 593, 2876
BsaXI ACNNNNNCTCC 6 cut(s) 244, 274, 400, 430, 1636, 1666
Bsc4I CCNNNNNNNGG 6 cut(s) 221, 346, 425, 545, 2727, 2876
Bse118I RCCGGY 2 cut(s) 163, 593
Bse1I ACTGG 5 cut(s) 96, 1685, 1767, 2296, 2376
Bse3DI GCAATG 5 cut(s) 152, 445, 1055, 1332, 2454
BseAI TCCGGA 1 cut(s) 456
BseBI CCWGG 2 cut(s) 1697, 2566
BseCI ATCGAT 1 cut(s) 2177
BseDI CCNNGG 6 cut(s) 693, 1380, 1695, 1696, 2103, 2564
BseLI CCNNNNNNNGG 6 cut(s) 221, 346, 425, 545, 2727, 2876
BseMI GCAATG 5 cut(s) 152, 445, 1055, 1332, 2454
BseMII CTCAG 6 cut(s) 174, 719, 1070, 1629, 2613, 2737
BseNI ACTGG 5 cut(s) 96, 1685, 1767, 2296, 2376
BseRI GAGGAG 1 cut(s) 1657
BseSI GKGCMC 3 cut(s) 133, 681, 2733
BseXI GCAGC 3 cut(s) 67, 1166, 2724
BseYI CCCAGC 1 cut(s) 2503
BsgI GTGCAG 2 cut(s) 53, 74
Bsh1285I CGRYCG 1 cut(s) 2181
BshFI GGCC 3 cut(s) 29, 102, 1054
BshNI GGYRCC 1 cut(s) 2435
BshTI ACCGGT 2 cut(s) 163, 593
BshVI ATCGAT 1 cut(s) 2177
BsiEI CGRYCG 1 cut(s) 2181
BsiHKAI GWGCWC 4 cut(s) 133, 191, 1034, 2291
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 6 cut(s) 134, 164, 457, 594, 2103, 2877
BslFI GGGAC 2 cut(s) 540, 1678
BslI CCNNNNNNNGG 6 cut(s) 221, 346, 425, 545, 2727, 2876
BsmAI GTCTC 5 cut(s) 82, 1127, 2618, 2879, 2923
BsmBI CGTCTC 1 cut(s) 2879
BsmFI GGGAC 2 cut(s) 540, 1678
BsmI GAATGC 2 cut(s) 13, 1102
BsnI GGCC 3 cut(s) 29, 102, 1054
Bso31I GGTCTC 2 cut(s) 1127, 2618
BsoBI CYCGRG 1 cut(s) 190
Bsp1286I GDGCHC 6 cut(s) 133, 191, 681, 1034, 2291, 2733
Bsp13I TCCGGA 1 cut(s) 456
Bsp1407I TGTACA 2 cut(s) 2429, 2473
BspACI CCGC 7 cut(s) 215, 255, 552, 686, 2519, 2955, 2967
BspANI GGCC 3 cut(s) 29, 102, 1054
BspCNI CTCAG 6 cut(s) 175, 720, 1069, 1630, 2614, 2736
BspDI ATCGAT 1 cut(s) 2177
BspEI TCCGGA 1 cut(s) 456
BspHI TCATGA 1 cut(s) 2257
BspLI GGNNCC 3 cut(s) 556, 2437, 2860
BspMAI CTGCAG 1 cut(s) 119
BspOI GCTAGC 1 cut(s) 1104
BspPI GGATC 8 cut(s) 45, 253, 611, 1667, 1956, 2354, 2676, 2861
BspT107I GGYRCC 1 cut(s) 2435
BspTNI GGTCTC 2 cut(s) 1127, 2618
BsrBI CCGCTC 1 cut(s) 255
BsrDI GCAATG 5 cut(s) 152, 445, 1055, 1332, 2454
BsrFI RCCGGY 2 cut(s) 163, 593
BsrGI TGTACA 2 cut(s) 2429, 2473
BsrI ACTGG 5 cut(s) 96, 1685, 1767, 2296, 2376
BssAI RCCGGY 2 cut(s) 163, 593
BssECI CCNNGG 6 cut(s) 693, 1380, 1695, 1696, 2103, 2564
BssNAI GTATAC 1 cut(s) 161
BssSI CACGAG 1 cut(s) 273
BssT1I CCWWGG 2 cut(s) 693, 1380
Bst1107I GTATAC 1 cut(s) 161
Bst2BI CACGAG 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 1697, 2566
Bst6I CTCTTC 2 cut(s) 1485, 1767
BstAPI GCANNNNNTGC 1 cut(s) 835
BstAUI TGTACA 2 cut(s) 2429, 2473
BstBAI YACGTR 1 cut(s) 1871
BstC8I GCNNGC 6 cut(s) 81, 129, 690, 1102, 1290, 2710
BstDEI CTNAG 8 cut(s) 183, 567, 728, 1056, 1638, 2021, 2622, 2723
BstEII GGTNACC 2 cut(s) 589, 2582
BstHHI GCGC 1 cut(s) 1294
BstMAI GTCTC 5 cut(s) 82, 1127, 2618, 2879, 2923
BstMCI CGRYCG 1 cut(s) 2181
BstMWI GCNNNNNNNGC 5 cut(s) 605, 685, 835, 1385, 2214
BstNI CCWGG 2 cut(s) 1697, 2566
BstNSI RCATGY 4 cut(s) 83, 109, 447, 1719
BstPI GGTNACC 2 cut(s) 589, 2582
BstSCI CCNGG 4 cut(s) 133, 1695, 2102, 2564
BstSFI CTRYAG 2 cut(s) 115, 1258
BstSLI GKGCMC 3 cut(s) 133, 681, 2733
BstSNI TACGTA 1 cut(s) 1871
BstV1I GCAGC 3 cut(s) 67, 1166, 2724
BstV2I GAAGAC 1 cut(s) 1416
BstX2I RGATCY 5 cut(s) 1133, 2032, 2359, 2668, 2822
BstXI CCANNNNNNTGG 1 cut(s) 2614
BstYI RGATCY 5 cut(s) 1133, 2032, 2359, 2668, 2822
BstZ17I GTATAC 1 cut(s) 161
Bsu15I ATCGAT 1 cut(s) 2177
BsuI GTATCC 2 cut(s) 246, 464
BsuRI GGCC 3 cut(s) 29, 102, 1054
BsuTUI ATCGAT 1 cut(s) 2177
BtgZI GCGATG 1 cut(s) 2077
BtrI CACGTC 1 cut(s) 1430
BtsI GCAGTG 2 cut(s) 18, 2682
BtsIMutI CAGTG 8 cut(s) 18, 660, 978, 2141, 2289, 2383, 2682, 2731
Cac8I GCNNGC 6 cut(s) 81, 129, 690, 1102, 1290, 2710
CciI TCATGA 1 cut(s) 2257
CfoI GCGC 1 cut(s) 1294
Cfr10I RCCGGY 2 cut(s) 163, 593
Cfr13I GGNCC 4 cut(s) 536, 554, 2693, 2903
ClaI ATCGAT 1 cut(s) 2177
CspAI ACCGGT 2 cut(s) 163, 593
CspCI CAANNNNNGTGG 6 cut(s) 427, 462, 1149, 1184, 2928, 2963
DdeI CTNAG 8 cut(s) 183, 567, 728, 1056, 1638, 2021, 2622, 2723
DraIII CACNNNGTG 1 cut(s) 137
EaeI YGGCCR 2 cut(s) 27, 100
Eam1104I CTCTTC 2 cut(s) 1485, 1767
EarI CTCTTC 2 cut(s) 1485, 1767
Ecl136II GAGCTC 1 cut(s) 189
Eco105I TACGTA 1 cut(s) 1871
Eco130I CCWWGG 2 cut(s) 693, 1380
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 2 cut(s) 1127, 2618
Eco47I GGWCC 4 cut(s) 536, 554, 2693, 2903
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 4 cut(s) 44, 1236, 1872, 2232
Eco88I CYCGRG 1 cut(s) 190
Eco91I GGTNACC 2 cut(s) 589, 2582
EcoICRI GAGCTC 1 cut(s) 189
EcoO65I GGTNACC 2 cut(s) 589, 2582
EcoRI GAATTC 1 cut(s) 1347
EcoRII CCWGG 2 cut(s) 1695, 2564
EcoT14I CCWWGG 2 cut(s) 693, 1380
EcoT22I ATGCAT 2 cut(s) 445, 1717
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 2 cut(s) 693, 1380
Esp3I CGTCTC 1 cut(s) 2879
FalI AAGNNNNNCTT 2 cut(s) 1571, 1603
FaqI GGGAC 2 cut(s) 540, 1678
FauI CCCGC 3 cut(s) 222, 248, 2960
FbaI TGATCA 2 cut(s) 1801, 2254
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 4 cut(s) 56, 1155, 2713, 2955
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 4 cut(s) 56, 1155, 2713, 2955
FspBI CTAG 5 cut(s) 603, 911, 1035, 1092, 1101
FspI TGCGCA 1 cut(s) 1293
GlaI GCGC 1 cut(s) 1293
GluI GCNGC 4 cut(s) 56, 1155, 2713, 2955
GsaI CCCAGC 1 cut(s) 2507
GsuI CTGGAG 2 cut(s) 402, 2947
HaeIII GGCC 3 cut(s) 29, 102, 1054
HapII CCGG 6 cut(s) 134, 164, 457, 594, 2103, 2877
HhaI GCGC 1 cut(s) 1294
Hin6I GCGC 1 cut(s) 1292
HinP1I GCGC 1 cut(s) 1292
HincII GTYRAC 2 cut(s) 1788, 2344
HindII GTYRAC 2 cut(s) 1788, 2344
HindIII AAGCTT 2 cut(s) 1725, 2331
HpaII CCGG 6 cut(s) 134, 164, 457, 594, 2103, 2877
HphI GGTGA 3 cut(s) 149, 1171, 2576
Hpy166II GTNNAC 8 cut(s) 131, 161, 211, 272, 670, 1788, 2344, 2474
Hpy8I GTNNAC 8 cut(s) 131, 161, 211, 272, 670, 1788, 2344, 2474
HpyAV CCTTC 8 cut(s) 505, 852, 873, 1223, 1573, 1597, 1827, 2255
HpyCH4IV ACGT 5 cut(s) 672, 739, 1429, 1870, 2654
HpyF10VI GCNNNNNNNGC 5 cut(s) 605, 685, 835, 1385, 2214
HpyF3I CTNAG 8 cut(s) 183, 567, 728, 1056, 1638, 2021, 2622, 2723
HpySE526I ACGT 5 cut(s) 672, 739, 1429, 1870, 2654
HspAI GCGC 1 cut(s) 1292
Kpn2I TCCGGA 1 cut(s) 456
Ksp22I TGATCA 2 cut(s) 1801, 2254
LmnI GCTCC 6 cut(s) 252, 976, 1162, 1958, 2409, 2864
Lsp1109I GCAGC 3 cut(s) 67, 1166, 2724
LweI GCATC 9 cut(s) 114, 412, 1511, 1513, 1573, 1614, 1731, 2648, 2928
MaeI CTAG 5 cut(s) 603, 911, 1035, 1092, 1101
MaeII ACGT 5 cut(s) 672, 739, 1429, 1870, 2654
MaeIII GTNAC 6 cut(s) 377, 589, 1318, 1404, 2042, 2582
MbiI CCGCTC 1 cut(s) 255
MfeI CAATTG 1 cut(s) 1952
MflI RGATCY 5 cut(s) 1133, 2032, 2359, 2668, 2822
MhlI GDGCHC 6 cut(s) 133, 191, 681, 1034, 2291, 2733
MlsI TGGCCA 1 cut(s) 102
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 7 cut(s) 202, 285, 1295, 1700, 1787, 1933, 2612
MmeI TCCRAC 3 cut(s) 148, 1170, 1457
Mox20I TGGCCA 1 cut(s) 102
Mph1103I ATGCAT 2 cut(s) 445, 1717
MroI TCCGGA 1 cut(s) 456
MscI TGGCCA 1 cut(s) 102
MslI CAYNNNNRTG 6 cut(s) 665, 1325, 1559, 2566, 2736, 2978
Msp20I TGGCCA 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 2252
MspI CCGG 6 cut(s) 134, 164, 457, 594, 2103, 2877
MspR9I CCNGG 4 cut(s) 135, 1697, 2104, 2566
MunI CAATTG 1 cut(s) 1952
Mva1269I GAATGC 2 cut(s) 13, 1102
MvaI CCWGG 2 cut(s) 1697, 2566
MwoI GCNNNNNNNGC 5 cut(s) 605, 685, 835, 1385, 2214
NciI CCSGG 2 cut(s) 135, 2104
NheI GCTAGC 1 cut(s) 1100
NlaIV GGNNCC 3 cut(s) 556, 2437, 2860
NmuCI GTSAC 3 cut(s) 377, 1318, 2582
NsbI TGCGCA 1 cut(s) 1293
NsiI ATGCAT 2 cut(s) 445, 1717
NspI RCATGY 4 cut(s) 83, 109, 447, 1719
OliI CACNNNNGTG 2 cut(s) 665, 2566
PaeI GCATGC 1 cut(s) 83
PaeR7I CTCGAG 1 cut(s) 190
PagI TCATGA 1 cut(s) 2257
PasI CCCWGGG 1 cut(s) 1696
PciI ACATGT 1 cut(s) 105
PctI GAATGC 2 cut(s) 13, 1102
PfeI GAWTC 5 cut(s) 799, 1840, 1859, 2153, 2195
PflMI CCANNNNNTGG 1 cut(s) 545
PinAI ACCGGT 2 cut(s) 163, 593
PkrI GCNGC 4 cut(s) 57, 1156, 2714, 2956
Ple19I CGATCG 1 cut(s) 2181
PleI GAGTC 7 cut(s) 201, 284, 1295, 1699, 1786, 1933, 2612
PpsI GAGTC 7 cut(s) 201, 284, 1295, 1699, 1786, 1933, 2612
Ppu21I YACGTR 1 cut(s) 1871
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 3 cut(s) 1116, 2415, 2898
Psp124BI GAGCTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 672
Psp6I CCWGG 2 cut(s) 1695, 2564
PspEI GGTNACC 2 cut(s) 589, 2582
PspFI CCCAGC 1 cut(s) 2503
PspGI CCWGG 2 cut(s) 1695, 2564
PspN4I GGNNCC 3 cut(s) 556, 2437, 2860
PspPI GGNCC 4 cut(s) 536, 554, 2693, 2903
PspXI VCTCGAGB 1 cut(s) 190
PstI CTGCAG 1 cut(s) 119
PsuI RGATCY 5 cut(s) 1133, 2032, 2359, 2668, 2822
PvuI CGATCG 1 cut(s) 2181
PvuII CAGCTG 1 cut(s) 2252
RseI CAYNNNNRTG 6 cut(s) 665, 1325, 1559, 2566, 2736, 2978
SacI GAGCTC 1 cut(s) 191
SatI GCNGC 4 cut(s) 56, 1155, 2713, 2955
Sau96I GGNCC 4 cut(s) 536, 554, 2693, 2903
ScaI AGTACT 2 cut(s) 415, 2019
SchI GAGTC 7 cut(s) 202, 285, 1295, 1700, 1787, 1933, 2612
ScrFI CCNGG 4 cut(s) 135, 1697, 2104, 2566
SduI GDGCHC 6 cut(s) 133, 191, 681, 1034, 2291, 2733
SfaNI GCATC 9 cut(s) 114, 412, 1511, 1513, 1573, 1614, 1731, 2648, 2928
SfcI CTRYAG 2 cut(s) 115, 1258
Sfr274I CTCGAG 1 cut(s) 190
SinI GGWCC 4 cut(s) 536, 554, 2693, 2903
SlaI CTCGAG 1 cut(s) 190
SmiMI CAYNNNNRTG 6 cut(s) 665, 1325, 1559, 2566, 2736, 2978
SmlI CTYRAG 4 cut(s) 190, 813, 1808, 2352
SmoI CTYRAG 4 cut(s) 190, 813, 1808, 2352
SnaBI TACGTA 1 cut(s) 1871
SphI GCATGC 1 cut(s) 83
SsiI CCGC 7 cut(s) 215, 255, 552, 686, 2519, 2955, 2967
SspI AATATT 3 cut(s) 491, 1798, 2554
SspMI CTAG 5 cut(s) 603, 911, 1035, 1092, 1101
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 133, 1695, 2102, 2564
StyI CCWWGG 2 cut(s) 693, 1380
TaiI ACGT 5 cut(s) 675, 742, 1432, 1873, 2657
TaqI TCGA 6 cut(s) 191, 1515, 2177, 2181, 2308, 2915
TatI WGTACW 6 cut(s) 413, 581, 1238, 2017, 2429, 2473
TauI GCSGC 1 cut(s) 2957
TfiI GAWTC 5 cut(s) 799, 1840, 1859, 2153, 2195
TscAI CASTG 8 cut(s) 18, 667, 978, 2148, 2296, 2383, 2689, 2731
TseFI GTSAC 3 cut(s) 377, 1318, 2582
TseI GCWGC 3 cut(s) 55, 1154, 2712
Tsp45I GTSAC 3 cut(s) 377, 1318, 2582
TspGWI ACGGA 3 cut(s) 59, 1961, 2903
TspRI CASTG 8 cut(s) 18, 667, 978, 2148, 2296, 2383, 2689, 2731
Van91I CCANNNNNTGG 1 cut(s) 545
VneI GTGCAC 1 cut(s) 129
VpaK11BI GGWCC 4 cut(s) 536, 554, 2693, 2903
VspI ATTAAT 3 cut(s) 1116, 2415, 2898
XapI RAATTY 6 cut(s) 983, 1347, 1446, 2050, 2609, 2852
XceI RCATGY 4 cut(s) 83, 109, 447, 1719
XcmI CCANNNNNNNNNTGG 1 cut(s) 2500
XhoI CTCGAG 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 160
XspI CTAG 5 cut(s) 603, 911, 1035, 1092, 1101
ZrmI AGTACT 2 cut(s) 415, 2019
Zsp2I ATGCAT 2 cut(s) 445, 1717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.