MD06G1101400.v1.1

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012794
Physical Location & Seq
Reverse (-)
23785412 .. 23792786
7375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1101400.v1.1.491

Sequence Viewer

Length: 3138 bp
ATGGAGGATGCAGTGGCTGAGGAAGTGAACCCATTAGATCCAGCAGAGGTTGCACAACTCCAAGGGAAAGAGAATGACTACTCACTAAAATCTGAAACCGGCAACCCATTGGAATGCCAAGAGATGCGTATACCAGTTGACGGTGATTATCCCTTAAGCTCGCAACATGAGATTTTAGAGAAAGTAGATATGGCTAGCTATGTTAACAAATTAGAACACCCATATAGTGGCCTAGGCTTTATGAATGATGTTGGTTTCATGGAGGAAGAGCTAACTCTGAGGAATTACAATAGTTCAAACCTAGCCATGCTTGATACATCAAACAATCAAGGAAGAATGCAATCTAGGCAGAACCCGCAACAACATTTATTTCAGCTAGCAAGTGGATCAGGAAGTGGGAGTTCACAAGTCAAAACTGCAGTCAAGGATAATGGGAAGGCAATTTCCGGTGGCTTGGAGAATGAGGGGGCCACATCTTTCCCTGGTTTTTGGAATAAAAGGGAATTAAGTGACAATCACTATGACGTTGTGGAAGAATTGACAAACATTGAAAATACGGGGGCTTCAGCCAACACTTATGCAGGTATTCGGACAAAAATCCTATCTAGGCCAGGATTTTCTGAGTTTTTTGTTAAAAGTACACTGAAGGGTAAGGGTGTCATATTGAAGGGTCCGAATCATGAGAATGGTCACATTGAATCTAGAAACCTGAATAGTTTGAAGGTGGCCGGTGATTCTTTGGCAGCTTCTAATCCAACAATGAGTATGAATGCAAATGTCGTTACGCCTTCTTCCAATGGTGTGAATGTCGGGCTTAGGGGTGGTGGCTCTAATCATGATGAAAACAGTTTGAGAGAATGGCTGAATATTGGACGTCCTAAAGCGAATAAAGTTGAATGCTTGTATATATTTAGACAAATAGTGGATCTGGTGGATAATTTTCATTCTAAAGGAGTTTCTTTGCCTGGATTGCGACCTTCTTTCTTCAAGTTGTTACCTTCAAATCAGGTTAAGTATGTTTGCTTACCAGTCCGGAACGAGATGCTTGAGAGTACGAGGGATAGAGATACATCCCTTTTAGAAAATAGTTCAATCAAGAAAAAGCAAAAGATAAGTCAGAACACTAGATTGCACTGGCCCCAGTTCTCTAGCACTGCCTACTTCATGCATGATAATGTGAATACCAGCCACATCAACATCGCTCGTTTGCAGAACAGAAGTGATGCATTTGATGAACATAATCCAAGAACAAGGCATGAGATACGTACCATATCCACCAATCCTCATATGGATTATGCAGCTCAGCGGTTTACTCCAATAAGCGAGCTATTGGAAGAGAAGTGGTATATTAGTCCTGAGGAACTCAGTGAAGGAAGTTGCACAATTTTTTCAAATATCTACAGTCTGGGTGTTCTTCTTTTTGAGTTACTTGCTCATTTTGACTCAAATAGTGCCCTTGCTGCCGCAATGTCCAATCTGCGCCACAGAATTCTTCCCCCAAATTTCCTGTCAGAAAATGGTAAGGAGGTTGGATTTATTATGTGGCTTCTTCATCCTGAACCATCTTCACGCCCAACAACAAGGCAAATTCTACAATCTGAAGTAGTTAACAGGTTACAGGAGGTTTCTGTAAAAGAATTGTCATCTTCTGTGGAACAAGAGGGTGCAGAATTGGATTTATTATTGCATTTCCTTACTTCTTTGAAAGAACAGAAGCAGAACCATGCCGCAAATTTGTTGGAAACCATTGGGTTCATAGAAGCAGATGTTGAAGAAGTGGAGAGGAGGCACTCTCTAAGAAAACCCTTGATTGATCGTGGCTTGTACGATGAATCCATCAGTGGAAGTAAATATATGTTCGTGAGCAAAGAAGATTCAAGGTCAGAGGTGCTTTCTCCATTATTTCCTGTTCCTAGTTCAAATGAGTCGAGGTTGATGAAAAACATAGATCAGTTTGAGAGTGCTTACTTCTCGATGAGATCAAGAATTCAGATTCCTGAGACTGATTCTAGAGTATGCAGAGATAAAGATTTATTAAGAACTCGCAATAATTGGTATGTGGGAACAAAGGATGAAGACAAGGAGACATGCACTGATCGACTGGGAGCCATCTTTGATGGTTTGTGTAAATATTCGCGGTATAGTAAGTTTGAGGTACGTGGCATACTAAGAAATGGAGATTTCAGCAGTTCCTCTAAAGTAATTTCTTCTATGAGTTTTGATCGGGATGAAGACTATTTTGCTACTGCTGGGGTTTCAATGAAAATTAAGATTTTTGAGTTTAATGCTTTCTTCAACAATTCTGTTGATATTCATTATCCGGTGATTGAGATGTCAAACAAATCAAGGATCACATGTGTTTGCTGGAACAACTACATAAAAAACTATCTGGCTTCATCTGATTATGATGGTGTAATCAAGTTATGGGATGCCAGCACTGGTCAAGAGTTTGCTCAATACACTGAGCATGTAACGAGGGCTTGGTCTGTAGACTTTTCTCAAGTATATCCAACAAAATTAGCCAGTGGAAGTGATGATGGTTCTGTAAAATTGTGGAGCATCAACGAGAAAAACTGTTTAGATACCATCAAGCACGCTGCAAATGTATGCTGTGTACAGTTCTCTGCTCACTCCTCTCATTTGCTGGCCTTTGGGAGTGCGGACTACCACACCTACTGCTATGATCTTCGTAATACTAAAATTCCATGGTGCGTACTGGATGGGCATAAGAAAGCTGTAAGCTTTGTGAAATTTTTGGACTCGGAGACCCTTGTTTCTGCATCCACTGACAGTACATTAAAGCTATGGGATCTTAACAGAACCAGTACGAGTGATCCGTCCACCGATGCTTGCAGCCTAACCCTTGGTGGGCATACTAATGAGAAGAACTTTGTCGGGTTATCCGTTTCAGATGGTTACATAGCATGTGGTTCAGAAACGAATGAGGTTTATGCTTACTATAGATCTCTGCCTATGCCGATCACTTCACACAAGTTTGGCTCCATTGATCCTATTTCTGGAAAGGAGACTGATGATTATAGTGCACAATGGGTTTCGAGTGTCTGCTGGAGAGGGAAATCAGACATGGTTGTCGCTGCCAATTCAGACGGGTGTATAAAAGTGTTGGAGATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

1046

Amino Acids

117.17

Weight (kDa)

5.6

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 443 - 534 6.3e-06 Protein kinase domain
Beta-prop_WDR3_1st PF25173 728 - 834 1.7e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 730 - 986 5.7e-32 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 731 - 828 6.2e-09 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 770 - 936 5.6e-12 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 778 - 890 2.8e-15 THOC3 beta-propeller domain
WD40_Prp19 PF24814 779 - 866 1e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 779 - 936 1e-18 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 781 - 864 5.5e-07 WDHD1 first WD40 domain
WD40_Gbeta PF25391 788 - 898 1e-07 G protein beta WD-40 repeat protein
EIF3I PF24805 795 - 884 7.8e-09 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 838 - 943 1.8e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 838 - 986 1.7e-20 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 839 - 953 5.4e-09 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 839 - 943 5.5e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 839 - 940 8.2e-06 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 840 - 941 1.7e-09 TEP-1 second beta-propeller
WDR55 PF24796 840 - 940 3.7e-08 WDR55
Beta-prop_EIPR1 PF23609 861 - 936 2.1e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 865 - 943 5.2e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 866 - 979 1.3e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 882 - 1043 7.7e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3089
AatII GACGTC 1 cut(s) 877
Acc36I ACCTGC 1 cut(s) 572
AccB7I CCANNNNNTGG 1 cut(s) 227
AccI GTMKAC 2 cut(s) 130, 2490
AccII CGCG 1 cut(s) 2137
AccIII TCCGGA 1 cut(s) 1032
AciI CCGC 6 cut(s) 356, 1306, 1464, 1728, 2137, 2660
AclWI GGATC 7 cut(s) 32, 394, 933, 2357, 2817, 2828, 3002
AcoI YGGCCR 1 cut(s) 726
AcsI RAATTY 7 cut(s) 1488, 1501, 1587, 1732, 1986, 2700, 2750
AcuI CTGAAG 3 cut(s) 549, 665, 1620
AcyI GRCGYC 1 cut(s) 874
AfaI GTAC 9 cut(s) 640, 1054, 1267, 1826, 2157, 2616, 2715, 2794, 2827
AfiI CCNNNNNNNGG 4 cut(s) 140, 227, 2868, 3017
AflII CTTAAG 1 cut(s) 154
AflIII ACRYGT 1 cut(s) 2354
AjnI CCWGG 3 cut(s) 481, 610, 964
Alw21I GWGCWC 1 cut(s) 3046
Alw26I GTCTC 4 cut(s) 1994, 2078, 2759, 3020
Alw44I GTGCAC 1 cut(s) 3042
AlwI GGATC 7 cut(s) 32, 394, 933, 2357, 2817, 2828, 3002
AlwNI CAGNNNCTG 1 cut(s) 17
Aor13HI TCCGGA 1 cut(s) 1032
AoxI GGCC 6 cut(s) 229, 468, 608, 726, 1136, 2646
ApaLI GTGCAC 1 cut(s) 3042
ApeKI GCWGC 6 cut(s) 743, 1298, 1460, 2597, 2853, 3095
ApoI RAATTY 7 cut(s) 1488, 1501, 1587, 1732, 1986, 2700, 2750
ArsI GACNNNNNNTTYG 6 cut(s) 405, 437, 1099, 1131, 1493, 1525
AspA2I CCTAGG 1 cut(s) 232
AspLEI GCGC 1 cut(s) 1482
AspS9I GGNCC 3 cut(s) 468, 671, 1137
AsuHPI GGTGA 3 cut(s) 155, 743, 2335
AsuNHI GCTAGC 2 cut(s) 194, 376
AvaII GGWCC 1 cut(s) 671
AvrII CCTAGG 1 cut(s) 232
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 2 cut(s) 1456, 3046
BbsI GAAGAC 2 cut(s) 2082, 2238
Bbv12I GWGCWC 1 cut(s) 3046
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 6 cut(s) 755, 1310, 1447, 2584, 2865, 3082
BccI CCATC 9 cut(s) 1570, 1844, 2111, 2117, 2402, 2531, 2594, 2714, 2906
BciT130I CCWGG 3 cut(s) 483, 612, 966
BcoDI GTCTC 4 cut(s) 1994, 2078, 2759, 3020
BfmI CTRYAG 4 cut(s) 417, 1399, 2487, 2959
BfrI CTTAAG 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 572
BglII AGATCT 1 cut(s) 2963
BisI GCNGC 8 cut(s) 744, 1299, 1461, 1464, 1728, 2598, 2854, 3096
BlnI CCTAGG 1 cut(s) 232
BlpI GCTNAGC 1 cut(s) 1302
BlsI GCNGC 8 cut(s) 745, 1300, 1462, 1465, 1729, 2599, 2855, 3097
Bme1390I CCNGG 3 cut(s) 483, 612, 966
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 3 cut(s) 468, 671, 1137
BmiI GGNNCC 5 cut(s) 469, 672, 1139, 2107, 3001
BmrFI CCNGG 3 cut(s) 483, 612, 966
BmrI ACTGGG 2 cut(s) 1135, 2111
BmsI GCATC 7 cut(s) 114, 1032, 1213, 2419, 2568, 2789, 2836
BmtI GCTAGC 2 cut(s) 198, 380
BmuI ACTGGG 2 cut(s) 1135, 2111
BpiI GAAGAC 2 cut(s) 2082, 2238
BplI GAGNNNNNCTC 2 cut(s) 1777, 1809
BpmI CTGGAG 1 cut(s) 3088
Bpu10I CCTNAGC 2 cut(s) 18, 815
Bpu1102I GCTNAGC 1 cut(s) 1302
BpuEI CTTGAG 2 cut(s) 1067, 2484
BsaAI YACGTR 2 cut(s) 1265, 2159
BsaHI GRCGYC 1 cut(s) 874
BsaI GGTCTC 1 cut(s) 2759
BsaJI CCNNGG 5 cut(s) 61, 232, 481, 2705, 2863
BsaWI WCCGGW 3 cut(s) 446, 1032, 2320
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 227, 2868, 3017
Bse118I RCCGGY 2 cut(s) 98, 728
Bse1I ACTGG 9 cut(s) 134, 1028, 1139, 1141, 2106, 2443, 2523, 2721, 2823
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 1 cut(s) 1473
BseAI TCCGGA 1 cut(s) 1032
BseBI CCWGG 3 cut(s) 483, 612, 966
BseDI CCNNGG 5 cut(s) 61, 232, 481, 2705, 2863
BseGI GGATG 8 cut(s) 13, 1070, 1552, 2077, 2233, 2434, 2725, 2780
BseLI CCNNNNNNNGG 4 cut(s) 140, 227, 2868, 3017
BseMI GCAATG 1 cut(s) 1473
BseMII CTCAG 8 cut(s) 9, 269, 612, 1316, 1347, 1378, 1989, 2454
BseNI ACTGG 9 cut(s) 134, 1028, 1139, 1141, 2106, 2443, 2523, 2721, 2823
BseRI GAGGAG 2 cut(s) 1798, 2623
BseSI GKGCMC 2 cut(s) 1456, 3046
BseXI GCAGC 6 cut(s) 755, 1310, 1447, 2584, 2865, 3082
BseYI CCCAGC 1 cut(s) 2249
BsgI GTGCAG 1 cut(s) 1686
Bsh1236I CGCG 1 cut(s) 2137
BshFI GGCC 6 cut(s) 231, 470, 610, 728, 1138, 2648
BsiHKAI GWGCWC 1 cut(s) 3046
BsiSI CCGG 5 cut(s) 99, 447, 729, 1033, 2321
BslI CCNNNNNNNGG 4 cut(s) 140, 227, 2868, 3017
BsmAI GTCTC 4 cut(s) 1994, 2078, 2759, 3020
BsmI GAATGC 4 cut(s) 119, 342, 775, 902
BsnI GGCC 6 cut(s) 231, 470, 610, 728, 1138, 2648
Bso31I GGTCTC 1 cut(s) 2759
Bsp1286I GDGCHC 2 cut(s) 1456, 3046
Bsp13I TCCGGA 1 cut(s) 1032
Bsp1407I TGTACA 1 cut(s) 2614
Bsp1720I GCTNAGC 1 cut(s) 1302
Bsp19I CCATGG 1 cut(s) 2705
BspACI CCGC 6 cut(s) 356, 1306, 1464, 1728, 2137, 2660
BspANI GGCC 6 cut(s) 231, 470, 610, 728, 1138, 2648
BspCNI CTCAG 8 cut(s) 10, 270, 613, 1315, 1348, 1377, 1990, 2455
BspEI TCCGGA 1 cut(s) 1032
BspFNI CGCG 1 cut(s) 2137
BspHI TCATGA 2 cut(s) 679, 835
BspLI GGNNCC 5 cut(s) 469, 672, 1139, 2107, 3001
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 572
BspOI GCTAGC 2 cut(s) 198, 380
BspPI GGATC 7 cut(s) 32, 394, 933, 2357, 2817, 2828, 3002
BspQI GCTCTTC 1 cut(s) 261
BspTI CTTAAG 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 2759
BsrDI GCAATG 1 cut(s) 1473
BsrFI RCCGGY 2 cut(s) 98, 728
BsrGI TGTACA 1 cut(s) 2614
BsrI ACTGG 9 cut(s) 134, 1028, 1139, 1141, 2106, 2443, 2523, 2721, 2823
BssAI RCCGGY 2 cut(s) 98, 728
BssECI CCNNGG 5 cut(s) 61, 232, 481, 2705, 2863
BssNAI GTATAC 1 cut(s) 131
BssNI GRCGYC 1 cut(s) 874
BssT1I CCWWGG 4 cut(s) 61, 232, 2705, 2863
Bst1107I GTATAC 1 cut(s) 131
Bst2UI CCWGG 3 cut(s) 483, 612, 966
Bst4CI ACNGT 6 cut(s) 143, 848, 1403, 2576, 2619, 2792
Bst6I CTCTTC 2 cut(s) 261, 1329
BstACI GRCGYC 1 cut(s) 874
BstAFI CTTAAG 1 cut(s) 154
BstAPI GCANNNNNTGC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 2614
BstBAI YACGTR 2 cut(s) 1265, 2159
BstC8I GCNNGC 8 cut(s) 161, 196, 378, 1325, 2434, 2595, 2646, 2851
BstDSI CCRYGG 1 cut(s) 2705
BstF5I GGATG 8 cut(s) 13, 1070, 1552, 2077, 2233, 2434, 2725, 2780
BstFNI CGCG 1 cut(s) 2137
BstHHI GCGC 1 cut(s) 1482
BstMAI GTCTC 4 cut(s) 1994, 2078, 2759, 3020
BstMWI GCNNNNNNNGC 5 cut(s) 50, 346, 355, 970, 1460
BstNI CCWGG 3 cut(s) 483, 612, 966
BstNSI RCATGY 4 cut(s) 2091, 2358, 2471, 2928
BstSCI CCNGG 3 cut(s) 481, 610, 964
BstSFI CTRYAG 4 cut(s) 417, 1399, 2487, 2959
BstSLI GKGCMC 2 cut(s) 1456, 3046
BstSNI TACGTA 1 cut(s) 1265
BstUI CGCG 1 cut(s) 2137
BstV1I GCAGC 6 cut(s) 755, 1310, 1447, 2584, 2865, 3082
BstV2I GAAGAC 2 cut(s) 2082, 2238
BstX2I RGATCY 4 cut(s) 37, 925, 2809, 2963
BstYI RGATCY 4 cut(s) 37, 925, 2809, 2963
BstZ17I GTATAC 1 cut(s) 131
Bsu36I CCTNAGG 1 cut(s) 1356
BsuRI GGCC 6 cut(s) 231, 470, 610, 728, 1138, 2648
BtgI CCRYGG 1 cut(s) 2705
BtgZI GCGATG 1 cut(s) 1183
BtsCI GGATG 8 cut(s) 13, 1070, 1552, 2077, 2233, 2434, 2725, 2780
BtsI GCAGTG 2 cut(s) 18, 1152
BveI ACCTGC 1 cut(s) 572
Cac8I GCNNGC 8 cut(s) 161, 196, 378, 1325, 2434, 2595, 2646, 2851
CaiI CAGNNNCTG 1 cut(s) 17
CciI TCATGA 2 cut(s) 679, 835
CfoI GCGC 1 cut(s) 1482
Cfr10I RCCGGY 2 cut(s) 98, 728
Cfr13I GGNCC 3 cut(s) 468, 671, 1137
Csp6I GTAC 9 cut(s) 639, 1053, 1266, 1825, 2156, 2615, 2714, 2793, 2826
CviQI GTAC 9 cut(s) 639, 1053, 1266, 1825, 2156, 2615, 2714, 2793, 2826
DrdI GACNNNNNNGTC 1 cut(s) 3089
DseDI GACNNNNNNGTC 1 cut(s) 3089
EaeI YGGCCR 1 cut(s) 726
Eam1104I CTCTTC 2 cut(s) 261, 1329
EarI CTCTTC 2 cut(s) 261, 1329
Eco105I TACGTA 1 cut(s) 1265
Eco130I CCWWGG 4 cut(s) 61, 232, 2705, 2863
Eco31I GGTCTC 1 cut(s) 2759
Eco47I GGWCC 1 cut(s) 671
Eco57I CTGAAG 3 cut(s) 549, 665, 1620
Eco81I CCTNAGG 1 cut(s) 1356
EcoRI GAATTC 2 cut(s) 1488, 1986
EcoRII CCWGG 3 cut(s) 481, 610, 964
EcoT14I CCWWGG 4 cut(s) 61, 232, 2705, 2863
EcoT22I ATGCAT 2 cut(s) 1170, 1228
ErhI CCWWGG 4 cut(s) 61, 232, 2705, 2863
FauI CCCGC 1 cut(s) 363
FauNDI CATATG 1 cut(s) 1287
FblI GTMKAC 2 cut(s) 130, 2490
Fnu4HI GCNGC 8 cut(s) 744, 1299, 1461, 1464, 1728, 2598, 2854, 3096
FokI GGATG 8 cut(s) 20, 1057, 1539, 2084, 2240, 2441, 2732, 2767
Fsp4HI GCNGC 8 cut(s) 744, 1299, 1461, 1464, 1728, 2598, 2854, 3096
GlaI GCGC 1 cut(s) 1481
GluI GCNGC 8 cut(s) 744, 1299, 1461, 1464, 1728, 2598, 2854, 3096
GsaI CCCAGC 1 cut(s) 2253
GsuI CTGGAG 1 cut(s) 3088
HaeIII GGCC 6 cut(s) 231, 470, 610, 728, 1138, 2648
HapII CCGG 5 cut(s) 99, 447, 729, 1033, 2321
HhaI GCGC 1 cut(s) 1482
Hin1I GRCGYC 1 cut(s) 874
Hin6I GCGC 1 cut(s) 1480
HinP1I GCGC 1 cut(s) 1480
HincII GTYRAC 3 cut(s) 139, 205, 1609
HindII GTYRAC 3 cut(s) 139, 205, 1609
HindIII AAGCTT 1 cut(s) 2740
HpaI GTTAAC 2 cut(s) 205, 1609
HpaII CCGG 5 cut(s) 99, 447, 729, 1033, 2321
HphI GGTGA 3 cut(s) 155, 743, 2335
HpyAV CCTTC 8 cut(s) 430, 640, 661, 715, 798, 987, 1008, 1364
HpyCH4III ACNGT 6 cut(s) 143, 848, 1403, 2576, 2619, 2792
HpyCH4IV ACGT 4 cut(s) 525, 874, 1264, 2158
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 346, 355, 970, 1460
HpySE526I ACGT 4 cut(s) 525, 874, 1264, 2158
Hsp92I GRCGYC 1 cut(s) 874
HspAI GCGC 1 cut(s) 1480
Kpn2I TCCGGA 1 cut(s) 1032
KspAI GTTAAC 2 cut(s) 205, 1609
LguI GCTCTTC 1 cut(s) 261
LmnI GCTCC 3 cut(s) 2105, 2556, 3005
Lsp1109I GCAGC 6 cut(s) 755, 1310, 1447, 2584, 2865, 3082
LweI GCATC 7 cut(s) 114, 1032, 1213, 2419, 2568, 2789, 2836
MaeII ACGT 4 cut(s) 525, 874, 1264, 2158
MaeIII GTNAC 8 cut(s) 509, 689, 781, 993, 1425, 1614, 2470, 2915
MflI RGATCY 4 cut(s) 37, 925, 2809, 2963
MhlI GDGCHC 2 cut(s) 1456, 3046
MlyI GAGTC 3 cut(s) 1436, 1934, 2753
MmeI TCCRAC 5 cut(s) 779, 1510, 1719, 2534, 3105
Mph1103I ATGCAT 2 cut(s) 1170, 1228
MroI TCCGGA 1 cut(s) 1032
MslI CAYNNNNRTG 4 cut(s) 112, 684, 1173, 2877
MspA1I CMGCKG 1 cut(s) 1306
MspCI CTTAAG 1 cut(s) 154
MspI CCGG 5 cut(s) 99, 447, 729, 1033, 2321
MspR9I CCNGG 3 cut(s) 483, 612, 966
Mva1269I GAATGC 4 cut(s) 119, 342, 775, 902
MvaI CCWGG 3 cut(s) 483, 612, 966
MvnI CGCG 1 cut(s) 2137
MwoI GCNNNNNNNGC 5 cut(s) 50, 346, 355, 970, 1460
NcoI CCATGG 1 cut(s) 2705
NdeI CATATG 1 cut(s) 1287
NheI GCTAGC 2 cut(s) 194, 376
NlaIV GGNNCC 5 cut(s) 469, 672, 1139, 2107, 3001
NmuCI GTSAC 2 cut(s) 509, 689
NsiI ATGCAT 2 cut(s) 1170, 1228
NspI RCATGY 4 cut(s) 2091, 2358, 2471, 2928
PagI TCATGA 2 cut(s) 679, 835
PciI ACATGT 1 cut(s) 2354
PciSI GCTCTTC 1 cut(s) 261
PcsI WCGNNNNNNNCGW 2 cut(s) 2834, 2901
PctI GAATGC 4 cut(s) 119, 342, 775, 902
PfeI GAWTC 7 cut(s) 676, 698, 734, 1832, 1874, 1993, 2006
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 8 cut(s) 745, 1300, 1462, 1465, 1729, 2599, 2855, 3097
PleI GAGTC 3 cut(s) 1436, 1933, 2753
PpsI GAGTC 3 cut(s) 1436, 1933, 2753
Ppu21I YACGTR 2 cut(s) 1265, 2159
PscI ACATGT 1 cut(s) 2354
Psp6I CCWGG 3 cut(s) 481, 610, 964
PspFI CCCAGC 1 cut(s) 2249
PspGI CCWGG 3 cut(s) 481, 610, 964
PspN4I GGNNCC 5 cut(s) 469, 672, 1139, 2107, 3001
PspPI GGNCC 3 cut(s) 468, 671, 1137
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 4 cut(s) 37, 925, 2809, 2963
RsaI GTAC 9 cut(s) 640, 1054, 1267, 1826, 2157, 2616, 2715, 2794, 2827
RsaNI GTAC 9 cut(s) 639, 1053, 1266, 1825, 2156, 2615, 2714, 2793, 2826
RseI CAYNNNNRTG 4 cut(s) 112, 684, 1173, 2877
SapI GCTCTTC 1 cut(s) 261
SatI GCNGC 8 cut(s) 744, 1299, 1461, 1464, 1728, 2598, 2854, 3096
Sau96I GGNCC 3 cut(s) 468, 671, 1137
SchI GAGTC 3 cut(s) 1436, 1934, 2753
ScrFI CCNGG 3 cut(s) 483, 612, 966
SduI GDGCHC 2 cut(s) 1456, 3046
SfaNI GCATC 7 cut(s) 114, 1032, 1213, 2419, 2568, 2789, 2836
SfcI CTRYAG 4 cut(s) 417, 1399, 2487, 2959
SinI GGWCC 1 cut(s) 671
SmiMI CAYNNNNRTG 4 cut(s) 112, 684, 1173, 2877
SmlI CTYRAG 3 cut(s) 154, 1046, 2499
SmoI CTYRAG 3 cut(s) 154, 1046, 2499
SnaBI TACGTA 1 cut(s) 1265
SsiI CCGC 6 cut(s) 356, 1306, 1464, 1728, 2137, 2660
SspI AATATT 2 cut(s) 868, 2132
StyD4I CCNGG 3 cut(s) 481, 610, 964
StyI CCWWGG 4 cut(s) 61, 232, 2705, 2863
TaaI ACNGT 6 cut(s) 143, 848, 1403, 2576, 2619, 2792
TaiI ACGT 4 cut(s) 528, 877, 1267, 2161
TaqI TCGA 4 cut(s) 1928, 1973, 2098, 3056
TatI WGTACW 3 cut(s) 638, 2614, 2792
TauI GCSGC 2 cut(s) 1466, 1730
TfiI GAWTC 7 cut(s) 676, 698, 734, 1832, 1874, 1993, 2006
TseFI GTSAC 2 cut(s) 509, 689
TseI GCWGC 6 cut(s) 743, 1298, 1460, 2597, 2853, 3095
Tsp45I GTSAC 2 cut(s) 509, 689
TspGWI ACGGA 2 cut(s) 2826, 2893
Van91I CCANNNNNTGG 1 cut(s) 227
Vha464I CTTAAG 1 cut(s) 154
VneI GTGCAC 1 cut(s) 3042
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 7 cut(s) 1488, 1501, 1587, 1732, 1986, 2700, 2750
XbaI TCTAGA 2 cut(s) 701, 2009
XceI RCATGY 4 cut(s) 2091, 2358, 2471, 2928
XcmI CCANNNNNNNNNTGG 1 cut(s) 1285
XmaJI CCTAGG 1 cut(s) 232
XmiI GTMKAC 2 cut(s) 130, 2490
ZraI GACGTC 1 cut(s) 875
Zsp2I ATGCAT 2 cut(s) 1170, 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.