RLG00000004114

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012794
Physical Location & Seq
Reverse (-)
56313234 .. 56318304
5071 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004114

Sequence Viewer

Length: 2988 bp
ATGGAGAATGCAGTGGGTGGGGAAGCGACCCCACCTAATTCGACGGAGGGTGCACAGCTTCAGAGCAAGGAGAACGAGCATGCATTGAGACATGGAAAGGACCACATGTTCGACTACCAAGAGATGCGTATACCGGGTGGTGACGAAGATCATTCACTGTGTATACCAGTTGGAAGCAATTGTCCTGAGAGCTCAAGTCAGGAGTGTTTTGTAAAACCGTATGATGGCTTAGGCTTATATCCAAGCGGATCAAGGAGTGGGAATGGTGGGAGTTCACGAGTCAATACTGCATTCAGAGATTATGGTCAGATGAGATCAAATGATTTGGAGAAAGTGGAGTACCCATCTCTGGATGATATTATGGCTCAGAGGGCAAATAGTGATCATCTGGTTGAAGTTGTGGCGGAATTGACAAATACTGTAGATGGAGGGGTTTCAGGCAATTCATATGGAGGTATCCGGACAAAAATGCTTTCCAAATCAAGATCAGAATTTTATGTTAAGTCTACATTGAAGGGTAAGGGTGTTATTTTCAAAGGTCCAAATCATGATGGCGGTCATGTTGAATCTAAGAACCTTAACAGTACAAAGGTTACTGGTGGTTCTAAGGCTGCTTTTGATTCAGTACAGAGTTTGGATGCCAAAATTGTTATGCCTTCTTCCAATGATGTGAACGTTGTGCCCAAACCACCAGCCTTGGAACATGATGAAATCAGTTTAAGAGAATGGCTGAGAATTGGACGTCCTAATGTCAATAAAGTTGAGTGTCTGTATGTATTCAAACAAATTGCAAATCTCGTGGATCATTTTCACTCTCAAGGAGTTGCTTTGCAAGAATTGCGCCCTTCTTTTTTTAAGGTGTCACCTTCAAATGAGGTCAAGTATGTTGGCTTACCAATCCCAAAAGAGATATTAGAAAGTTCTATGGATCAAGGTATCTGTCATTCAGAAAATAGTCCAATGAGAAAAATGTCAGGTGAGCAAGAATTTGCTTCTGTTGGTTCCAGTGCAAAGAAGCAAAAGATCAACCGGAACACTAGATTAGTTAGGCAATGGCCTCAGTTCCCCACTAGTTCCCGTTTCCAACGTGAAACTAGGAATACTAGCCACATTAACATCAATGGTCTGCAAAATAGAAGTCCTCATGTGTATAATGCGGCTCAACAACACTTGACTTCAATCACTGAGCAATTGGAAGAAAAGTGGTATATTAGTCCTGAAGAACTCAGTGAAGGGAGCTGTACAGTTTTGTCAAATATCTACAGTCTGGGCGTTCTTCTTTTTGAGTTGCTGGCCCGTTTTGACTCAAATAGTGCTGTTGTCTCAGCAATGTTCAATCTGCGTCATAGAATTCTTCCGCCAAATTTTCTGTCAGAAAACGCTCAAGAGGCTGGATTTTGTCTTTGGTTACTTCATCCTGAACCGTCTTCACGTCCAACAACAAGGGAAATTCTACAATCAGAAGTAGTTAATGGATTACAGGAGATATGTGAAGAGAAAGCGGCATCATCTGTAGTCGAAGAGGATGCTGAATTGGAACTATTATCCCATTTCCTCACGTCACTGAAAGAAAAGAAGCTGAAGGATGCCTCCAAGTTGATGGCAAGCATTAGGGTTTTAGAAGCAGATGCTGAAGAAGCTGAGAGGAGGCACTATTCAAGAAATCCCGACTGGTACAAAGAGTCCCTCGGTGGATGGAAAAACGCATGTAAAGAAGCTTCAAGATCAGAGGTGCTTTCTCCATTACCCCCTCTTCCTAGTTCAAATGAGTCAAGGTTGACAAGGAATATTGAGCAGCTTGAGAGTGCTTACTTCTCTATGAGATCTAGGATTCAGCCTCCTGAAGCTGATTGTACTATACGCATAGATAAAGAGTTATTAAAATCTCGCAAGAATTGGTATTTGGAATCAGATGATGACGAAAAGAAGACTCCTACCTATCAATTGGGAGCTATCTTTGATGGTTTATGCAAGTATGATCGGTACAGTAAGTTTGAAGTATGTGGAGTACTAAGAAATGGAGATTTCAACAGTAACTCAAATTTGATTTGCTCTTTGAGTTTTGATCGGGATGAGGACTACATCGCTTCTGCTGGGGTTTCAAAGAAAATAAAGATTTATGAGTTCAATGCACTTTTCAATGATTCTGTTGATATTCATTATCCATCGATCGAGATGTCAAATGAATCAAAGATTAGCTGTATTTGCTGGAACAACTACATAAAAAACTATCTCACTTCAACTGATTATGAAGGTGTAGTGAAGTTGTGGGATGTCAGCACCAGTCAAGAGTTTGCTCGATACAGAGGGCATGAACAAAAGGCTTGGTCTGTTGACTTCTCTCAAGTAGATCCTACAAAATTAGCCAGCGGGAGTGATGACGGTTCTGTGAAACTCTGGAGCATTAATGAGAAAAACTGTTTAGGCACCATCAGAAACAGAAACATTGCAAATGTATGCTGTGTACAGTTCTCTGCTCACTCCACTAATTTGCTGGCCTTTGGGTCTGCGGACTACAACACATATTGCTATGATCTTCGTAATACTAGAACGCCCTGGTGTGTATTGGTTGGTCACCAAAAAAGTGTAAGCTATGTCAAATTCTTGGACTCTGAGACCCTTGTTTCTGCATCCACTGACAACACATTAAAGCTATGGGATCTCAACAAAACCACTGCCAGTGGTCCATCCACCAATGCTTGCAGCTTAACCCTCAGTGGGCATACCAATGAGAAGAACTTTGTGGGCTTATCCGTTTCTAATGGTTATATAGCATGCGGTTCAGAAACAAATGAGGTCTATGCTTATTATAGATCTCTTTCTATGCCGATCACTTCATACAAATTTGGCTCCATTGATCCTATAACCGGAAAGGAGACTGATGATATTAATGGATCATTTGTTTCAAGTGTCTGCTGGAGACAGAAATCAAACATGGTGGTTGCCGCCAATTCAAGCGGGTGCATAAAAGTGTTGCAGATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

996

Amino Acids

111.02

Weight (kDa)

5.84

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 394 - 488 2.8e-06 Protein kinase domain
Beta-prop_WDR3_1st PF25173 679 - 845 1.4e-15 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 679 - 936 5.8e-32 WDR5 beta-propeller domain
WDR55 PF24796 681 - 812 1.8e-06 WDR55
Beta-prop_TEP1_2nd PF25047 682 - 801 9.4e-06 TEP-1 second beta-propeller
Beta-prop_EML PF23409 683 - 891 2.4e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 683 - 849 5.7e-08 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 684 - 839 1.4e-16 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 685 - 804 2.6e-06 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 688 - 777 5.2e-08 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 694 - 886 9.8e-14 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 728 - 814 7.2e-08 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 746 - 892 1.4e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 754 - 811 5.5e-06 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 763 - 891 8.2e-09 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 766 - 801 9.2e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 775 - 893 6.6e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 786 - 936 1.1e-19 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 788 - 891 1.3e-10 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 814 - 990 1.3e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 817 - 929 1.4e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 818 - 993 6.4e-11 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 745
AccB1I GGYRCC 1 cut(s) 2426
AccI GTMKAC 3 cut(s) 130, 163, 506
AccIII TCCGGA 1 cut(s) 459
AclI AACGTT 1 cut(s) 675
AclWI GGATC 7 cut(s) 256, 810, 936, 2345, 2667, 2852, 2902
AcsI RAATTY 8 cut(s) 491, 986, 1350, 1363, 1449, 2041, 2600, 2843
AcuI CTGAAG 5 cut(s) 44, 1239, 1601, 1653, 1863
AcyI GRCGYC 1 cut(s) 742
AfaI GTAC 9 cut(s) 341, 586, 627, 1243, 1676, 1855, 1985, 2010, 2466
AfiI CCNNNNNNNGG 4 cut(s) 224, 349, 2718, 2867
AflIII ACRYGT 1 cut(s) 105
AhlI ACTAGT 1 cut(s) 1070
AjiI CACGTC 2 cut(s) 1433, 1560
AjnI CCWGG 1 cut(s) 2555
AjuI GAANNNNNNNTTGG 2 cut(s) 1886, 1918
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
Alw21I GWGCWC 2 cut(s) 55, 194
Alw26I GTCTC 5 cut(s) 82, 1327, 2609, 2870, 2914
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 7 cut(s) 256, 810, 936, 2345, 2667, 2852, 2902
AlwNI CAGNNNCTG 1 cut(s) 1631
Aor13HI TCCGGA 1 cut(s) 459
AoxI GGCC 3 cut(s) 1055, 1293, 2496
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 3 cut(s) 611, 1795, 2703
ApoI RAATTY 8 cut(s) 491, 986, 1350, 1363, 1449, 2041, 2600, 2843
ArsI GACNNNNNNTTYG 6 cut(s) 92, 124, 1123, 1155, 1355, 1387
AseI ATTAAT 2 cut(s) 2406, 2889
AspLEI GCGC 1 cut(s) 843
AspS9I GGNCC 4 cut(s) 100, 539, 1294, 2684
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 4 cut(s) 152, 855, 989, 2567
AvaII GGWCC 3 cut(s) 100, 539, 2684
BaeGI GKGCMC 2 cut(s) 55, 684
BanI GGYRCC 1 cut(s) 2426
BanII GRGCYC 1 cut(s) 194
BarI GAAGNNNNNNTAC 2 cut(s) 1702, 1734
BauI CACGAG 2 cut(s) 276, 797
BbsI GAAGAC 2 cut(s) 1419, 1934
Bbv12I GWGCWC 2 cut(s) 55, 194
BbvI GCAGC 3 cut(s) 598, 1807, 2715
BcgI CGANNNNNNTGC 2 cut(s) 1508, 1542
BciT130I CCWGG 1 cut(s) 2557
BciVI GTATCC 1 cut(s) 467
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 5 cut(s) 82, 1327, 2609, 2870, 2914
BcuI ACTAGT 1 cut(s) 1070
BfaI CTAG 7 cut(s) 1038, 1071, 1095, 1104, 1758, 1827, 2547
BfmI CTRYAG 3 cut(s) 420, 1261, 1512
BfuI GTATCC 1 cut(s) 467
BglII AGATCT 2 cut(s) 1823, 2813
BisI GCNGC 6 cut(s) 612, 1158, 1503, 1796, 2704, 2946
BlsI GCNGC 6 cut(s) 613, 1159, 1504, 1797, 2705, 2947
BmcAI AGTACT 1 cut(s) 2010
Bme1390I CCNGG 2 cut(s) 135, 2557
Bme18I GGWCC 3 cut(s) 100, 539, 2684
BmgBI CACGTC 2 cut(s) 1433, 1560
BmgT120I GGNCC 4 cut(s) 100, 539, 1294, 2684
BmiI GGNNCC 3 cut(s) 1003, 2428, 2851
BmrFI CCNGG 2 cut(s) 135, 2557
BmsI GCATC 7 cut(s) 114, 628, 1514, 1516, 1576, 1618, 2639
BpiI GAAGAC 2 cut(s) 1419, 1934
BpmI CTGGAG 2 cut(s) 2419, 2938
Bpu10I CCTNAGC 1 cut(s) 229
BpuEI CTTGAG 5 cut(s) 178, 801, 1368, 1820, 2328
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 2168
BsaHI GRCGYC 1 cut(s) 742
BsaI GGTCTC 1 cut(s) 2609
BsaJI CCNNGG 3 cut(s) 696, 1687, 2555
BsaWI WCCGGW 3 cut(s) 459, 1029, 2867
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 349, 2718, 2867
Bse1I ACTGG 6 cut(s) 167, 601, 1005, 1676, 2283, 2679
Bse3DI GCAATG 3 cut(s) 1058, 1335, 2445
BseAI TCCGGA 1 cut(s) 459
BseBI CCWGG 1 cut(s) 2557
BseCI ATCGAT 1 cut(s) 2168
BseDI CCNNGG 3 cut(s) 696, 1687, 2555
BseLI CCNNNNNNNGG 4 cut(s) 224, 349, 2718, 2867
BseMI GCAATG 3 cut(s) 1058, 1335, 2445
BseNI ACTGG 6 cut(s) 167, 601, 1005, 1676, 2283, 2679
BseRI GAGGAG 1 cut(s) 1660
BseSI GKGCMC 2 cut(s) 55, 684
BseXI GCAGC 3 cut(s) 598, 1807, 2715
BseYI CCCAGC 1 cut(s) 2093
Bsh1285I CGRYCG 1 cut(s) 2172
BshFI GGCC 3 cut(s) 1057, 1295, 2498
BshNI GGYRCC 1 cut(s) 2426
BshVI ATCGAT 1 cut(s) 2168
BsiEI CGRYCG 1 cut(s) 2172
BsiHKAI GWGCWC 2 cut(s) 55, 194
BsiSI CCGG 4 cut(s) 134, 460, 1030, 2868
BslFI GGGAC 1 cut(s) 1669
BslI CCNNNNNNNGG 4 cut(s) 224, 349, 2718, 2867
BsmAI GTCTC 5 cut(s) 82, 1327, 2609, 2870, 2914
BsmFI GGGAC 1 cut(s) 1669
BsmI GAATGC 2 cut(s) 13, 290
BsnI GGCC 3 cut(s) 1057, 1295, 2498
Bso31I GGTCTC 1 cut(s) 2609
Bsp1286I GDGCHC 3 cut(s) 55, 194, 684
Bsp13I TCCGGA 1 cut(s) 459
Bsp1407I TGTACA 2 cut(s) 1241, 2464
BspANI GGCC 3 cut(s) 1057, 1295, 2498
BspDI ATCGAT 1 cut(s) 2168
BspEI TCCGGA 1 cut(s) 459
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 3 cut(s) 1003, 2428, 2851
BspPI GGATC 7 cut(s) 256, 810, 936, 2345, 2667, 2852, 2902
BspT107I GGYRCC 1 cut(s) 2426
BspTNI GGTCTC 1 cut(s) 2609
BsrDI GCAATG 3 cut(s) 1058, 1335, 2445
BsrGI TGTACA 2 cut(s) 1241, 2464
BsrI ACTGG 6 cut(s) 167, 601, 1005, 1676, 2283, 2679
BssECI CCNNGG 3 cut(s) 696, 1687, 2555
BssNAI GTATAC 2 cut(s) 131, 164
BssNI GRCGYC 1 cut(s) 742
BssSI CACGAG 2 cut(s) 276, 797
BssT1I CCWWGG 1 cut(s) 696
Bst1107I GTATAC 2 cut(s) 131, 164
Bst2BI CACGAG 2 cut(s) 276, 797
Bst2UI CCWGG 1 cut(s) 2557
Bst6I CTCTTC 3 cut(s) 1488, 1515, 1758
BstACI GRCGYC 1 cut(s) 742
BstAPI GCANNNNNTGC 1 cut(s) 838
BstAUI TGTACA 2 cut(s) 1241, 2464
BstC8I GCNNGC 7 cut(s) 81, 1293, 1606, 2368, 2496, 2701, 2776
BstEII GGTNACC 1 cut(s) 2573
BstHHI GCGC 1 cut(s) 843
BstMAI GTCTC 5 cut(s) 82, 1327, 2609, 2870, 2914
BstMCI CGRYCG 1 cut(s) 2172
BstMWI GCNNNNNNNGC 5 cut(s) 371, 838, 1388, 1637, 2205
BstNI CCWGG 1 cut(s) 2557
BstNSI RCATGY 4 cut(s) 83, 109, 1710, 2778
BstPI GGTNACC 1 cut(s) 2573
BstSCI CCNGG 2 cut(s) 133, 2555
BstSFI CTRYAG 3 cut(s) 420, 1261, 1512
BstSLI GKGCMC 2 cut(s) 55, 684
BstV1I GCAGC 3 cut(s) 598, 1807, 2715
BstV2I GAAGAC 2 cut(s) 1419, 1934
BstX2I RGATCY 4 cut(s) 1823, 2350, 2659, 2813
BstXI CCANNNNNNTGG 1 cut(s) 1600
BstYI RGATCY 4 cut(s) 1823, 2350, 2659, 2813
BstZ17I GTATAC 2 cut(s) 131, 164
Bsu15I ATCGAT 1 cut(s) 2168
BsuI GTATCC 1 cut(s) 467
BsuRI GGCC 3 cut(s) 1057, 1295, 2498
BsuTUI ATCGAT 1 cut(s) 2168
BtgZI GCGATG 1 cut(s) 2068
BtrI CACGTC 2 cut(s) 1433, 1560
BtsI GCAGTG 2 cut(s) 18, 2673
Cac8I GCNNGC 7 cut(s) 81, 1293, 1606, 2368, 2496, 2701, 2776
CaiI CAGNNNCTG 1 cut(s) 1631
CciI TCATGA 1 cut(s) 547
CfoI GCGC 1 cut(s) 843
Cfr13I GGNCC 4 cut(s) 100, 539, 1294, 2684
ClaI ATCGAT 1 cut(s) 2168
CseI GACGC 1 cut(s) 1331
Csp6I GTAC 9 cut(s) 340, 585, 626, 1242, 1675, 1854, 1984, 2009, 2465
CspCI CAANNNNNGTGG 6 cut(s) 678, 713, 780, 815, 2919, 2954
CviQI GTAC 9 cut(s) 340, 585, 626, 1242, 1675, 1854, 1984, 2009, 2465
Eam1104I CTCTTC 3 cut(s) 1488, 1515, 1758
EarI CTCTTC 3 cut(s) 1488, 1515, 1758
EciI GGCGGA 2 cut(s) 419, 1347
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 2609
Eco47I GGWCC 3 cut(s) 100, 539, 2684
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 5 cut(s) 44, 1239, 1601, 1653, 1863
Eco91I GGTNACC 1 cut(s) 2573
EcoICRI GAGCTC 1 cut(s) 192
EcoO65I GGTNACC 1 cut(s) 2573
EcoRI GAATTC 1 cut(s) 1350
EcoRII CCWGG 1 cut(s) 2555
EcoT14I CCWWGG 1 cut(s) 696
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 696
FaqI GGGAC 1 cut(s) 1669
FauI CCCGC 2 cut(s) 2363, 2951
FauNDI CATATG 1 cut(s) 448
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 3 cut(s) 130, 163, 506
Fnu4HI GCNGC 6 cut(s) 612, 1158, 1503, 1796, 2704, 2946
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 6 cut(s) 612, 1158, 1503, 1796, 2704, 2946
FspBI CTAG 7 cut(s) 1038, 1071, 1095, 1104, 1758, 1827, 2547
GlaI GCGC 1 cut(s) 842
GluI GCNGC 6 cut(s) 612, 1158, 1503, 1796, 2704, 2946
GsaI CCCAGC 1 cut(s) 2097
GsuI CTGGAG 2 cut(s) 2419, 2938
HaeIII GGCC 3 cut(s) 1057, 1295, 2498
HapII CCGG 4 cut(s) 134, 460, 1030, 2868
HgaI GACGC 1 cut(s) 1331
HhaI GCGC 1 cut(s) 843
Hin1I GRCGYC 1 cut(s) 742
Hin6I GCGC 1 cut(s) 841
HinP1I GCGC 1 cut(s) 841
HincII GTYRAC 2 cut(s) 1779, 2335
HindII GTYRAC 2 cut(s) 1779, 2335
HindIII AAGCTT 1 cut(s) 1716
HpaII CCGG 4 cut(s) 134, 460, 1030, 2868
HphI GGTGA 4 cut(s) 152, 855, 989, 2567
Hpy166II GTNNAC 9 cut(s) 53, 131, 164, 275, 507, 673, 1779, 2335, 2465
Hpy8I GTNNAC 9 cut(s) 53, 131, 164, 275, 507, 673, 1779, 2335, 2465
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 7 cut(s) 508, 666, 855, 876, 1226, 1576, 2246
HpyCH4IV ACGT 5 cut(s) 675, 742, 1087, 1432, 1559
HpyF10VI GCNNNNNNNGC 5 cut(s) 371, 838, 1388, 1637, 2205
HpySE526I ACGT 5 cut(s) 675, 742, 1087, 1432, 1559
Hsp92I GRCGYC 1 cut(s) 742
HspAI GCGC 1 cut(s) 841
Kpn2I TCCGGA 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 382
LmnI GCTCC 4 cut(s) 1236, 1949, 2400, 2855
Lsp1109I GCAGC 3 cut(s) 598, 1807, 2715
LweI GCATC 7 cut(s) 114, 628, 1514, 1516, 1576, 1618, 2639
MaeI CTAG 7 cut(s) 1038, 1071, 1095, 1104, 1758, 1827, 2547
MaeII ACGT 5 cut(s) 675, 742, 1087, 1432, 1559
MaeIII GTNAC 7 cut(s) 140, 592, 861, 1407, 1560, 2033, 2573
MfeI CAATTG 3 cut(s) 178, 1190, 1943
MflI RGATCY 4 cut(s) 1823, 2350, 2659, 2813
MhlI GDGCHC 3 cut(s) 55, 194, 684
MlyI GAGTC 6 cut(s) 288, 1298, 1691, 1778, 1924, 2603
MmeI TCCRAC 3 cut(s) 151, 1108, 1460
Mph1103I ATGCAT 1 cut(s) 85
MroI TCCGGA 1 cut(s) 459
MslI CAYNNNNRTG 2 cut(s) 2727, 2969
MspA1I CMGCKG 1 cut(s) 2370
MspI CCGG 4 cut(s) 134, 460, 1030, 2868
MspR9I CCNGG 2 cut(s) 135, 2557
MunI CAATTG 3 cut(s) 178, 1190, 1943
Mva1269I GAATGC 2 cut(s) 13, 290
MvaI CCWGG 1 cut(s) 2557
MwoI GCNNNNNNNGC 5 cut(s) 371, 838, 1388, 1637, 2205
NciI CCSGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 448
NlaIV GGNNCC 3 cut(s) 1003, 2428, 2851
NmuCI GTSAC 4 cut(s) 140, 861, 1560, 2573
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 4 cut(s) 83, 109, 1710, 2778
PaeI GCATGC 2 cut(s) 83, 2778
PagI TCATGA 1 cut(s) 547
PciI ACATGT 1 cut(s) 105
PctI GAATGC 2 cut(s) 13, 290
PfeI GAWTC 6 cut(s) 566, 620, 1831, 1907, 2144, 2186
PkrI GCNGC 6 cut(s) 613, 1159, 1504, 1797, 2705, 2947
Ple19I CGATCG 1 cut(s) 2172
PleI GAGTC 6 cut(s) 287, 1298, 1690, 1777, 1924, 2603
PpsI GAGTC 6 cut(s) 287, 1298, 1690, 1777, 1924, 2603
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 2 cut(s) 2406, 2889
Psp124BI GAGCTC 1 cut(s) 194
Psp1406I AACGTT 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 2555
PspEI GGTNACC 1 cut(s) 2573
PspFI CCCAGC 1 cut(s) 2093
PspGI CCWGG 1 cut(s) 2555
PspN4I GGNNCC 3 cut(s) 1003, 2428, 2851
PspPI GGNCC 4 cut(s) 100, 539, 1294, 2684
PstNI CAGNNNCTG 1 cut(s) 1631
PsuI RGATCY 4 cut(s) 1823, 2350, 2659, 2813
PvuI CGATCG 1 cut(s) 2172
RsaI GTAC 9 cut(s) 341, 586, 627, 1243, 1676, 1855, 1985, 2010, 2466
RsaNI GTAC 9 cut(s) 340, 585, 626, 1242, 1675, 1854, 1984, 2009, 2465
RseI CAYNNNNRTG 2 cut(s) 2727, 2969
SacI GAGCTC 1 cut(s) 194
SatI GCNGC 6 cut(s) 612, 1158, 1503, 1796, 2704, 2946
Sau96I GGNCC 4 cut(s) 100, 539, 1294, 2684
ScaI AGTACT 1 cut(s) 2010
SchI GAGTC 6 cut(s) 288, 1298, 1691, 1778, 1924, 2603
ScrFI CCNGG 2 cut(s) 135, 2557
SduI GDGCHC 3 cut(s) 55, 194, 684
SfaNI GCATC 7 cut(s) 114, 628, 1514, 1516, 1576, 1618, 2639
SfcI CTRYAG 3 cut(s) 420, 1261, 1512
SinI GGWCC 3 cut(s) 100, 539, 2684
SmiMI CAYNNNNRTG 2 cut(s) 2727, 2969
SmlI CTYRAG 5 cut(s) 193, 816, 1383, 1799, 2343
SmoI CTYRAG 5 cut(s) 193, 816, 1383, 1799, 2343
SpeI ACTAGT 1 cut(s) 1070
SphI GCATGC 2 cut(s) 83, 2778
SspI AATATT 1 cut(s) 1789
SspMI CTAG 7 cut(s) 1038, 1071, 1095, 1104, 1758, 1827, 2547
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 2 cut(s) 133, 2555
StyI CCWWGG 1 cut(s) 696
TaiI ACGT 5 cut(s) 678, 745, 1090, 1435, 1562
TaqI TCGA 6 cut(s) 41, 111, 1518, 2168, 2172, 2299
TatI WGTACW 6 cut(s) 584, 625, 1241, 1853, 2008, 2464
TauI GCSGC 3 cut(s) 1160, 1505, 2948
TfiI GAWTC 6 cut(s) 566, 620, 1831, 1907, 2144, 2186
TseFI GTSAC 4 cut(s) 140, 861, 1560, 2573
TseI GCWGC 3 cut(s) 611, 1795, 2703
Tsp45I GTSAC 4 cut(s) 140, 861, 1560, 2573
TspDTI ATGAA 8 cut(s) 435, 723, 1403, 2147, 2199, 2265, 2328, 2826
TspGWI ACGGA 2 cut(s) 59, 2743
VneI GTGCAC 1 cut(s) 51
VpaK11BI GGWCC 3 cut(s) 100, 539, 2684
VspI ATTAAT 2 cut(s) 2406, 2889
XapI RAATTY 8 cut(s) 491, 986, 1350, 1363, 1449, 2041, 2600, 2843
XceI RCATGY 4 cut(s) 83, 109, 1710, 2778
XcmI CCANNNNNNNNNTGG 1 cut(s) 2491
XmiI GTMKAC 3 cut(s) 130, 163, 506
XspI CTAG 7 cut(s) 1038, 1071, 1095, 1104, 1758, 1827, 2547
ZraI GACGTC 1 cut(s) 743
ZrmI AGTACT 1 cut(s) 2010
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.