Rw7G012930

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012794
Physical Location & Seq
Forward (+)
12297058 .. 12303377
6320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G012930.1

Sequence Viewer

Length: 2973 bp
ATGGAGAATGCAGTGGGTGGGGAAGCCACCCCACCTAATTCGACCGAGGGTGCACAGCTTCAGAGCAAGGAGAGCGAGCATGCATTGAGACATGGAAATGACCACATGTTCGACTACCAAGAGATGCGTACACCGGTTGGTGACGAAGATCATTCCCTGCGTATACCAGTTGGAAGCAATTGTCCTGAGAGCTCAAGTCAGGAGTGTTTTGTAAAACAACCGTATGATGGCTATCCAAGCGGATCAAGGAGCGGGAATGGTGGGAGTTCACGAGTCAATACTGCATTCAGAGATTATGGTCAGATGAGATCAAATGATTTGGAGAAAGTGGAGTACCCATCTCTGGATGATATTATGGCTCAGAGGCCAAATAGTGATAATCTGGTTGAAGTTGTGGCGGAATTGACAAATACTGTAGATGGAGGCGTTTCAGGCAATTCATATGGAGGTATCCGGACAAAAATGCTTTCCAAATCAAGATCAGAATTTTATGTTAAGTCTACATTGAAGGGTAAGGGTGTTATTTTCAAAGGTGATGGCGGTCATGTTGAATCTAAGAACCTTAACAGTACAAAGGTTAGTGGTGGTTCTAAGGCTACTTCTGATTCAATACAGAGTTTGGATGCCAAAATTGTTATGCCTTCTTCCAACGATCTGAACGTAGTGCCCAAACCACCAGCCTTGGAACATGATGAAATCAGTTTAAGAGAATGGCTGAGAATTGGACGTCCTAAAGTCAATAAAGTTGAGTGTCTGTATGTATTCAAACAAATTGCAAATCTCGTGGATCATTTTCACTCTCAAGGAGTTGCTTTGCAAGAATTGCGCCCTTCTTTTTTTAAGGTGTCACCTTCAAATGAGGTCAAGTATGTTGGCTTACCAATCCCAAAAGAGATAATAGAAAGTTCTATGGATCAAGGTATCTGTCATTCAGAAAATAGTCCAATGAGAAAAATGTCAGGTGAGCAAGAATTTGCTTCTGTTGGTTCCAGTGCAAAGAAGCAAAAGATCAGCCAGAACACTAGATTAGTTAGGCAATGGCCTCAGTTCCCCACTAGTTCCCGTTTCCAACGTGAAACTAGGAATGCTAGCCACATCAACATCAATGGTCTGCAAAATAGAAGTCCTCATATGTGTAATGCAGCTCAACAACACTTGACTTCAATCACTGAGCAATTGGAAGAAAAGTGGTATATTAGTCCTGAAGAACTCAATGAAGGGAGCTGTACAGTTTTGTCAAATATCTACAGTCTGGGCGTTCTTTTTTTTGAGTTGCTGGTCCGTTTTGACTCAAATAGTGCCATTGTCTCAGCAATGTTCAATCTGCGTCATAGAATTCTTCCTCCGAATTTTCTGTCAGAATACGCTAAAGAGGCTGGATTTTGTCTTTGGTTACTTCATCCTGAACCGTCTTCACGTCCAACAACAAGGGAAATTCTACAATCAGAAGTAGTTAATGGATTACAGGAGATGTGTGAAGAGAAAGTGGCATCATCTGTGGTCGAAGAGGATGCTGAATTGGAACTATTATCCCATTTCCTCACGTCACTGAAAGAAAAGAAGCAGAAGGATGCCTCCAAGTTGATGGCAAGCATTAGGGTTTTAGAAGCAGATGCTGAAGAAGCTGAGAGGAGGCACTATTCAAGAAATCCCGATTGGTACAAAGAGTCCCTCGGTGGATGGAAAAACGCATGTAAAGAAGCTTCCAGATCAGAGGTGCTTTCTCCATTACCCCCTCTTCCTAGTTCAAATGAGTCAAGGTTGACGAGGAATATTGATCAGCTTGAGAGTGCTTACTTCTCTATGAGATCTATGATTCAGCCTCCTGAAGCTGATTGTAATATACGCATAGATAAAGAGTTATTAAAATCTCGCAAGAATTGGTATTTGGAATCAGATGATGACGAAAAGAAGACTCCTACCGATCAATTGGGAGCAATCTTTGATGGTTTATGCAAGTATGATCGGTACAGTAAGTTTGAAGTATGTGGAGTACTAAGAAATGGAGATTTCAACAGTAACTCAAATTTGATTTGCTCTTTGAGTTTTGATCGGGATGAGGACTACATTGCTTCTGCTGGGGTTTCAAAGAAAATAAAGATTTATGAGTTCAATGCACTTTTCAATGATTCTGTTGATATTCATTATCCATCGATCGAGATGTCAAATGAATCAAAGATTAGCTGTATTTGCTGGAACAACTACATAAAAAACTATCTCACTTCAACTGATTATGAAGGTGTAGTGAAGTTGTGGGATGTCAGCACTGGTCAAGAGTTTGCTCGATACAGAGGGCATGAACAAAAGGCTTGGTCCGTTGACTTCTCTCAAGTAGATCCTACAAAATTAGCCAGCGGGAGTGATGACGGTTCTGTGAAACTCTGGAGCATTAATGAGAAAAACTGTTTAGGCACCATCAGAAACAGAAACATTGCAAATGTATGCTGTGTACAGTTCTCTGCTCACTCCACTAATTTGCTGGCCTTTGGGTCTGCGGACTACAACACATATTGCTATGATCTTCGTAATGCTAGAACGCCCTGGTGTGTATTGGTTGGTCACCAAAAAAGCGTAAGCTATGTCAAATTCTTGGACTCTGAGACCCTTGTTTCTGCATCCACTGACAACACATTAAAGCTATGGGATCTCAACAAAACCACTGCCAGTGGTCCATCCACCAATGCTTGCAGCTTAACCCTCAGTGGGCATACCAATGAGAAGAACTTTGTGGGCTTATCCGTTTCTAATGGTTATATAGCATGCGGTTCAGAAACAAATGAGGTCTATGCTTATTATAGATCTCTGCCTATGCCGATCACTTCATACAAATTTGGCTCCATTGATCCTATAACCGGAAAGGAGACTGATGATATTAATGGATCATTTGTTTCAAGTGTCTGCTGGAGACAGAAATCAAACATGGTGGTTGCCGCCAATTCAAGCGGGTGCATAAAAGTGTTGCAGATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

990

Amino Acids

110.54

Weight (kDa)

5.74

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 389 - 483 7.2e-06 Protein kinase domain
Beta-prop_WDR3_1st PF25173 674 - 840 8.6e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 674 - 931 2.6e-32 WDR5 beta-propeller domain
WDR55 PF24796 676 - 807 7e-07 WDR55
Beta-prop_TEP1_2nd PF25047 677 - 796 8.6e-06 TEP-1 second beta-propeller
WD40_Gbeta PF25391 678 - 844 6.3e-09 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 678 - 886 1.5e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 680 - 799 2.4e-06 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 683 - 767 8e-08 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 689 - 881 6e-14 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 721 - 835 9e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 722 - 809 5e-08 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 724 - 806 7.9e-07 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 730 - 887 1e-06 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 738 - 886 3.6e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 741 - 887 3e-12 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 741 - 829 2.2e-07 EIF3I
WD40 PF00400 761 - 796 7.4e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 769 - 888 4.1e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 781 - 931 1e-19 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 783 - 886 8.4e-11 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 809 - 984 3.5e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 812 - 924 1.9e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 814 - 988 1.9e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 730
AccB1I GGYRCC 1 cut(s) 2411
AccBSI CCGCTC 1 cut(s) 252
AccI GTMKAC 2 cut(s) 163, 500
AccIII TCCGGA 1 cut(s) 453
AciI CCGC 9 cut(s) 240, 252, 398, 540, 2355, 2495, 2763, 2931, 2943
AclWI GGATC 7 cut(s) 250, 795, 921, 2330, 2652, 2837, 2887
AcsI RAATTY 8 cut(s) 485, 971, 1335, 1348, 1434, 2026, 2585, 2828
AcuI CTGAAG 4 cut(s) 44, 1224, 1638, 1848
AcyI GRCGYC 1 cut(s) 727
AfaI GTAC 8 cut(s) 130, 335, 571, 1228, 1661, 1970, 1995, 2451
AfiI CCNNNNNNNGG 4 cut(s) 227, 343, 2703, 2852
AflIII ACRYGT 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 133
AhlI ACTAGT 1 cut(s) 1055
AjiI CACGTC 2 cut(s) 1418, 1545
AjnI CCWGG 1 cut(s) 2540
AjuI GAANNNNNNNTTGG 4 cut(s) 1639, 1671, 1871, 1903
Alw21I GWGCWC 2 cut(s) 55, 194
Alw26I GTCTC 5 cut(s) 82, 1312, 2594, 2855, 2899
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 7 cut(s) 250, 795, 921, 2330, 2652, 2837, 2887
AlwNI CAGNNNCTG 1 cut(s) 1616
Aor13HI TCCGGA 1 cut(s) 453
AoxI GGCC 3 cut(s) 365, 1040, 2481
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 1142, 2688
ApoI RAATTY 8 cut(s) 485, 971, 1335, 1348, 1434, 2026, 2585, 2828
ArsI GACNNNNNNTTYG 6 cut(s) 92, 124, 1108, 1140, 1340, 1372
AseI ATTAAT 2 cut(s) 2391, 2874
AsiGI ACCGGT 1 cut(s) 133
AspLEI GCGC 1 cut(s) 828
AspS9I GGNCC 3 cut(s) 1279, 2313, 2669
AsuHPI GGTGA 5 cut(s) 152, 545, 840, 974, 2552
AsuNHI GCTAGC 1 cut(s) 1088
AvaII GGWCC 3 cut(s) 1279, 2313, 2669
BaeGI GKGCMC 2 cut(s) 55, 669
BanI GGYRCC 1 cut(s) 2411
BanII GRGCYC 1 cut(s) 194
BarI GAAGNNNNNNTAC 2 cut(s) 1687, 1719
BauI CACGAG 2 cut(s) 270, 782
BbsI GAAGAC 2 cut(s) 1404, 1919
Bbv12I GWGCWC 2 cut(s) 55, 194
BbvI GCAGC 2 cut(s) 1154, 2700
BcgI CGANNNNNNTGC 2 cut(s) 1493, 1527
BciT130I CCWGG 1 cut(s) 2542
BciVI GTATCC 1 cut(s) 461
BclI TGATCA 1 cut(s) 1777
BcoDI GTCTC 5 cut(s) 82, 1312, 2594, 2855, 2899
BcuI ACTAGT 1 cut(s) 1055
BfaI CTAG 6 cut(s) 1023, 1056, 1080, 1089, 1743, 2532
BfmI CTRYAG 2 cut(s) 414, 1246
BfuI GTATCC 1 cut(s) 461
BglII AGATCT 2 cut(s) 1808, 2798
BisI GCNGC 3 cut(s) 1143, 2689, 2931
BlsI GCNGC 3 cut(s) 1144, 2690, 2932
BmcAI AGTACT 1 cut(s) 1995
Bme1390I CCNGG 1 cut(s) 2542
Bme18I GGWCC 3 cut(s) 1279, 2313, 2669
BmgBI CACGTC 2 cut(s) 1418, 1545
BmgT120I GGNCC 3 cut(s) 1279, 2313, 2669
BmiI GGNNCC 3 cut(s) 988, 2413, 2836
BmrFI CCNGG 1 cut(s) 2542
BmsI GCATC 7 cut(s) 114, 613, 1499, 1501, 1561, 1603, 2624
BmtI GCTAGC 1 cut(s) 1092
BpiI GAAGAC 2 cut(s) 1404, 1919
BpmI CTGGAG 2 cut(s) 2404, 2923
BpuEI CTTGAG 4 cut(s) 178, 786, 1805, 2313
Bsa29I ATCGAT 1 cut(s) 2153
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 727
BsaI GGTCTC 1 cut(s) 2594
BsaJI CCNNGG 4 cut(s) 45, 681, 1672, 2540
BsaWI WCCGGW 3 cut(s) 133, 453, 2852
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 343, 2703, 2852
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 4 cut(s) 167, 990, 2272, 2664
Bse3DI GCAATG 4 cut(s) 1043, 1320, 2067, 2430
Bse8I GATNNNNATC 1 cut(s) 231
BseAI TCCGGA 1 cut(s) 453
BseBI CCWGG 1 cut(s) 2542
BseCI ATCGAT 1 cut(s) 2153
BseDI CCNNGG 4 cut(s) 45, 681, 1672, 2540
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 4 cut(s) 227, 343, 2703, 2852
BseMI GCAATG 4 cut(s) 1043, 1320, 2067, 2430
BseMII CTCAG 9 cut(s) 177, 374, 707, 1058, 1161, 1323, 1617, 2589, 2713
BseNI ACTGG 4 cut(s) 167, 990, 2272, 2664
BseRI GAGGAG 1 cut(s) 1645
BseSI GKGCMC 2 cut(s) 55, 669
BseXI GCAGC 2 cut(s) 1154, 2700
BseYI CCCAGC 1 cut(s) 2078
Bsh1285I CGRYCG 2 cut(s) 45, 2157
BshFI GGCC 3 cut(s) 367, 1042, 2483
BshNI GGYRCC 1 cut(s) 2411
BshTI ACCGGT 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 2153
BsiEI CGRYCG 2 cut(s) 45, 2157
BsiHKAI GWGCWC 2 cut(s) 55, 194
BsiSI CCGG 3 cut(s) 134, 454, 2853
BslFI GGGAC 1 cut(s) 1654
BslI CCNNNNNNNGG 4 cut(s) 227, 343, 2703, 2852
BsmAI GTCTC 5 cut(s) 82, 1312, 2594, 2855, 2899
BsmFI GGGAC 1 cut(s) 1654
BsmI GAATGC 3 cut(s) 13, 284, 1090
BsnI GGCC 3 cut(s) 367, 1042, 2483
Bso31I GGTCTC 1 cut(s) 2594
Bsp1286I GDGCHC 3 cut(s) 55, 194, 669
Bsp13I TCCGGA 1 cut(s) 453
Bsp1407I TGTACA 2 cut(s) 1226, 2449
BspACI CCGC 9 cut(s) 240, 252, 398, 540, 2355, 2495, 2763, 2931, 2943
BspANI GGCC 3 cut(s) 367, 1042, 2483
BspCNI CTCAG 9 cut(s) 178, 373, 708, 1057, 1162, 1322, 1618, 2590, 2712
BspDI ATCGAT 1 cut(s) 2153
BspEI TCCGGA 1 cut(s) 453
BspLI GGNNCC 3 cut(s) 988, 2413, 2836
BspOI GCTAGC 1 cut(s) 1092
BspPI GGATC 7 cut(s) 250, 795, 921, 2330, 2652, 2837, 2887
BspT107I GGYRCC 1 cut(s) 2411
BspTNI GGTCTC 1 cut(s) 2594
BsrBI CCGCTC 1 cut(s) 252
BsrDI GCAATG 4 cut(s) 1043, 1320, 2067, 2430
BsrFI RCCGGY 1 cut(s) 133
BsrGI TGTACA 2 cut(s) 1226, 2449
BsrI ACTGG 4 cut(s) 167, 990, 2272, 2664
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 4 cut(s) 45, 681, 1672, 2540
BssNAI GTATAC 1 cut(s) 164
BssNI GRCGYC 1 cut(s) 727
BssSI CACGAG 2 cut(s) 270, 782
BssT1I CCWWGG 1 cut(s) 681
Bst1107I GTATAC 1 cut(s) 164
Bst2BI CACGAG 2 cut(s) 270, 782
Bst2UI CCWGG 1 cut(s) 2542
Bst6I CTCTTC 3 cut(s) 1473, 1500, 1743
BstACI GRCGYC 1 cut(s) 727
BstAPI GCANNNNNTGC 1 cut(s) 823
BstAUI TGTACA 2 cut(s) 1226, 2449
BstC8I GCNNGC 8 cut(s) 77, 81, 1090, 1591, 2353, 2481, 2686, 2761
BstEII GGTNACC 1 cut(s) 2558
BstHHI GCGC 1 cut(s) 828
BstMAI GTCTC 5 cut(s) 82, 1312, 2594, 2855, 2899
BstMCI CGRYCG 2 cut(s) 45, 2157
BstMWI GCNNNNNNNGC 7 cut(s) 72, 237, 432, 823, 1373, 1622, 2190
BstNI CCWGG 1 cut(s) 2542
BstNSI RCATGY 4 cut(s) 83, 109, 1695, 2763
BstPI GGTNACC 1 cut(s) 2558
BstSCI CCNGG 1 cut(s) 2540
BstSFI CTRYAG 2 cut(s) 414, 1246
BstSLI GKGCMC 2 cut(s) 55, 669
BstV1I GCAGC 2 cut(s) 1154, 2700
BstV2I GAAGAC 2 cut(s) 1404, 1919
BstX2I RGATCY 4 cut(s) 1808, 2335, 2644, 2798
BstXI CCANNNNNNTGG 1 cut(s) 1585
BstYI RGATCY 4 cut(s) 1808, 2335, 2644, 2798
BstZ17I GTATAC 1 cut(s) 164
Bsu15I ATCGAT 1 cut(s) 2153
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 3 cut(s) 367, 1042, 2483
BsuTUI ATCGAT 1 cut(s) 2153
BtrI CACGTC 2 cut(s) 1418, 1545
BtsI GCAGTG 2 cut(s) 18, 2658
BtsIMutI CAGTG 9 cut(s) 18, 997, 1167, 1547, 2265, 2619, 2658, 2671, 2707
Cac8I GCNNGC 8 cut(s) 77, 81, 1090, 1591, 2353, 2481, 2686, 2761
CaiI CAGNNNCTG 1 cut(s) 1616
CfoI GCGC 1 cut(s) 828
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 1279, 2313, 2669
ClaI ATCGAT 1 cut(s) 2153
CseI GACGC 1 cut(s) 1316
Csp6I GTAC 8 cut(s) 129, 334, 570, 1227, 1660, 1969, 1994, 2450
CspAI ACCGGT 1 cut(s) 133
CspCI CAANNNNNGTGG 6 cut(s) 663, 698, 765, 800, 2904, 2939
CviAII CATG 9 cut(s) 80, 92, 106, 545, 689, 1692, 2297, 2760, 2920
CviQI GTAC 8 cut(s) 129, 334, 570, 1227, 1660, 1969, 1994, 2450
Eam1104I CTCTTC 3 cut(s) 1473, 1500, 1743
EarI CTCTTC 3 cut(s) 1473, 1500, 1743
EciI GGCGGA 1 cut(s) 413
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 681
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 2594
Eco47I GGWCC 3 cut(s) 1279, 2313, 2669
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 4 cut(s) 44, 1224, 1638, 1848
Eco91I GGTNACC 1 cut(s) 2558
EcoICRI GAGCTC 1 cut(s) 192
EcoO65I GGTNACC 1 cut(s) 2558
EcoRI GAATTC 1 cut(s) 1335
EcoRII CCWGG 1 cut(s) 2540
EcoT14I CCWWGG 1 cut(s) 681
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 9 cut(s) 83, 95, 109, 548, 692, 1695, 2300, 2763, 2923
FaqI GGGAC 1 cut(s) 1654
FatI CATG 9 cut(s) 79, 91, 105, 544, 688, 1691, 2296, 2759, 2919
FauI CCCGC 3 cut(s) 245, 2348, 2936
FauNDI CATATG 2 cut(s) 442, 1131
FbaI TGATCA 1 cut(s) 1777
FblI GTMKAC 2 cut(s) 163, 500
Fnu4HI GCNGC 3 cut(s) 1143, 2689, 2931
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 1143, 2689, 2931
FspBI CTAG 6 cut(s) 1023, 1056, 1080, 1089, 1743, 2532
GlaI GCGC 1 cut(s) 827
GluI GCNGC 3 cut(s) 1143, 2689, 2931
GsaI CCCAGC 1 cut(s) 2082
GsuI CTGGAG 2 cut(s) 2404, 2923
HaeIII GGCC 3 cut(s) 367, 1042, 2483
HapII CCGG 3 cut(s) 134, 454, 2853
HgaI GACGC 1 cut(s) 1316
HhaI GCGC 1 cut(s) 828
Hin1I GRCGYC 1 cut(s) 727
Hin1II CATG 9 cut(s) 83, 95, 109, 548, 692, 1695, 2300, 2763, 2923
Hin6I GCGC 1 cut(s) 826
HinP1I GCGC 1 cut(s) 826
HincII GTYRAC 2 cut(s) 1764, 2320
HindII GTYRAC 2 cut(s) 1764, 2320
HindIII AAGCTT 1 cut(s) 1701
HpaII CCGG 3 cut(s) 134, 454, 2853
HphI GGTGA 5 cut(s) 152, 545, 840, 974, 2552
Hpy166II GTNNAC 8 cut(s) 53, 131, 164, 269, 501, 1764, 2320, 2450
Hpy8I GTNNAC 8 cut(s) 53, 131, 164, 269, 501, 1764, 2320, 2450
HpyAV CCTTC 7 cut(s) 502, 651, 840, 861, 1211, 1561, 2231
HpyCH4IV ACGT 5 cut(s) 660, 727, 1072, 1417, 1544
HpyF10VI GCNNNNNNNGC 7 cut(s) 72, 237, 432, 823, 1373, 1622, 2190
HpySE526I ACGT 5 cut(s) 660, 727, 1072, 1417, 1544
Hsp92I GRCGYC 1 cut(s) 727
Hsp92II CATG 9 cut(s) 83, 95, 109, 548, 692, 1695, 2300, 2763, 2923
HspAI GCGC 1 cut(s) 826
Kpn2I TCCGGA 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 1777
LmnI GCTCC 5 cut(s) 249, 1221, 1934, 2385, 2840
Lsp1109I GCAGC 2 cut(s) 1154, 2700
LweI GCATC 7 cut(s) 114, 613, 1499, 1501, 1561, 1603, 2624
MaeI CTAG 6 cut(s) 1023, 1056, 1080, 1089, 1743, 2532
MaeII ACGT 5 cut(s) 660, 727, 1072, 1417, 1544
MaeIII GTNAC 6 cut(s) 140, 846, 1392, 1545, 2018, 2558
MbiI CCGCTC 1 cut(s) 252
MfeI CAATTG 3 cut(s) 178, 1175, 1928
MflI RGATCY 4 cut(s) 1808, 2335, 2644, 2798
MhlI GDGCHC 3 cut(s) 55, 194, 669
MlyI GAGTC 6 cut(s) 282, 1283, 1676, 1763, 1909, 2588
MmeI TCCRAC 4 cut(s) 151, 672, 1093, 1445
Mph1103I ATGCAT 1 cut(s) 85
MroI TCCGGA 1 cut(s) 453
MslI CAYNNNNRTG 3 cut(s) 96, 2712, 2954
MspA1I CMGCKG 1 cut(s) 2355
MspI CCGG 3 cut(s) 134, 454, 2853
MspR9I CCNGG 1 cut(s) 2542
MunI CAATTG 3 cut(s) 178, 1175, 1928
Mva1269I GAATGC 3 cut(s) 13, 284, 1090
MvaI CCWGG 1 cut(s) 2542
MwoI GCNNNNNNNGC 7 cut(s) 72, 237, 432, 823, 1373, 1622, 2190
NdeI CATATG 2 cut(s) 442, 1131
NheI GCTAGC 1 cut(s) 1088
NlaIII CATG 9 cut(s) 83, 95, 109, 548, 692, 1695, 2300, 2763, 2923
NlaIV GGNNCC 3 cut(s) 988, 2413, 2836
NmuCI GTSAC 4 cut(s) 140, 846, 1545, 2558
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 4 cut(s) 83, 109, 1695, 2763
PaeI GCATGC 2 cut(s) 83, 2763
PciI ACATGT 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 657
PctI GAATGC 3 cut(s) 13, 284, 1090
PfeI GAWTC 6 cut(s) 551, 605, 1816, 1892, 2129, 2171
PinAI ACCGGT 1 cut(s) 133
PkrI GCNGC 3 cut(s) 1144, 2690, 2932
Ple19I CGATCG 1 cut(s) 2157
PleI GAGTC 6 cut(s) 281, 1283, 1675, 1762, 1909, 2588
PpsI GAGTC 6 cut(s) 281, 1283, 1675, 1762, 1909, 2588
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 2 cut(s) 2391, 2874
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 2540
PspEI GGTNACC 1 cut(s) 2558
PspFI CCCAGC 1 cut(s) 2078
PspGI CCWGG 1 cut(s) 2540
PspN4I GGNNCC 3 cut(s) 988, 2413, 2836
PspPI GGNCC 3 cut(s) 1279, 2313, 2669
PstNI CAGNNNCTG 1 cut(s) 1616
PsuI RGATCY 4 cut(s) 1808, 2335, 2644, 2798
PvuI CGATCG 1 cut(s) 2157
RsaI GTAC 8 cut(s) 130, 335, 571, 1228, 1661, 1970, 1995, 2451
RsaNI GTAC 8 cut(s) 129, 334, 570, 1227, 1660, 1969, 1994, 2450
RseI CAYNNNNRTG 3 cut(s) 96, 2712, 2954
SacI GAGCTC 1 cut(s) 194
SatI GCNGC 3 cut(s) 1143, 2689, 2931
Sau96I GGNCC 3 cut(s) 1279, 2313, 2669
ScaI AGTACT 1 cut(s) 1995
SchI GAGTC 6 cut(s) 282, 1283, 1676, 1763, 1909, 2588
ScrFI CCNGG 1 cut(s) 2542
SduI GDGCHC 3 cut(s) 55, 194, 669
SfaNI GCATC 7 cut(s) 114, 613, 1499, 1501, 1561, 1603, 2624
SfcI CTRYAG 2 cut(s) 414, 1246
SinI GGWCC 3 cut(s) 1279, 2313, 2669
SmiMI CAYNNNNRTG 3 cut(s) 96, 2712, 2954
SmlI CTYRAG 4 cut(s) 193, 801, 1784, 2328
SmoI CTYRAG 4 cut(s) 193, 801, 1784, 2328
SpeI ACTAGT 1 cut(s) 1055
SphI GCATGC 2 cut(s) 83, 2763
SsiI CCGC 9 cut(s) 240, 252, 398, 540, 2355, 2495, 2763, 2931, 2943
SspI AATATT 1 cut(s) 1774
SspMI CTAG 6 cut(s) 1023, 1056, 1080, 1089, 1743, 2532
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 2540
StyI CCWWGG 1 cut(s) 681
TaiI ACGT 5 cut(s) 663, 730, 1075, 1420, 1547
TaqI TCGA 6 cut(s) 41, 111, 1503, 2153, 2157, 2284
TaqII GACCGA 1 cut(s) 59
TatI WGTACW 4 cut(s) 569, 1226, 1993, 2449
TauI GCSGC 1 cut(s) 2933
TfiI GAWTC 6 cut(s) 551, 605, 1816, 1892, 2129, 2171
TscAI CASTG 9 cut(s) 18, 997, 1174, 1554, 2272, 2626, 2665, 2671, 2707
TseFI GTSAC 4 cut(s) 140, 846, 1545, 2558
TseI GCWGC 2 cut(s) 1142, 2688
Tsp45I GTSAC 4 cut(s) 140, 846, 1545, 2558
TspDTI ATGAA 9 cut(s) 429, 708, 1230, 1388, 2132, 2184, 2250, 2313, 2811
TspGWI ACGGA 3 cut(s) 1271, 2305, 2728
TspRI CASTG 9 cut(s) 18, 997, 1174, 1554, 2272, 2626, 2665, 2671, 2707
VneI GTGCAC 1 cut(s) 51
VpaK11BI GGWCC 3 cut(s) 1279, 2313, 2669
VspI ATTAAT 2 cut(s) 2391, 2874
XapI RAATTY 8 cut(s) 485, 971, 1335, 1348, 1434, 2026, 2585, 2828
XceI RCATGY 4 cut(s) 83, 109, 1695, 2763
XcmI CCANNNNNNNNNTGG 1 cut(s) 2476
XmiI GTMKAC 2 cut(s) 163, 500
XspI CTAG 6 cut(s) 1023, 1056, 1080, 1089, 1743, 2532
ZraI GACGTC 1 cut(s) 728
ZrmI AGTACT 1 cut(s) 1995
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.