RchiOBHm_Chr7g0195501

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012794
Physical Location & Seq
Forward (+)
13340758 .. 13347296
6539 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17485

Sequence Viewer

Length: 2844 bp
ATGGAGAATGCAGTGGGTGGGGAAGCCAACCCACCTAATTCGACCGAGGGTGCACAGCTTCAGAGCAAGCAGAGCGAGCATGCATTGAGACATGGAAATGACCACATGTTCGACTACCAAGAGATGCGTACACCGGTTGGTGACGAAGATCATTCCCTGCGTATACCAGTTGGAAGCAATTGTCCTGAGAGCTCAAGTCAGGAGTACCCATGTCTGGATGATATTATGGCTCAGAGGCCAAATAGTGATAATCTGGTTGAAGTTGTGGCGGAATTGACAAATACTGTAGATGGAGGCGTTTCAGGCAATTCATATGGAGGTATCCGGACAAAAATGCTTTCCAAATCAAGATCAGAATTTTATGTTAAGTCTACATTGAAGGGTAAGGGTGTTATTTTCAAAGGTGATGGCGGTCATGTTGAATCTAAGAACCTTAACAGTACAAAGGTTACTGGTGGTTCTAAGGCTACTTCTGATTCAATACAGAGTTTGGATGCCAAAATTGTTATGCCTTCTTCCAATGATCTGAACGCTGTGCCCAAACCACCAGCCTTGGAACATGATGAGATCAGCTTAAGAGAATGGCTGAGAATTGGACGTCCTAAAGTCAATAAAGTTGAGTGTCTGTATGTATTCAAACAAATTGCAAATCTCGTGGATCATTTTCACTCTCAAGGAGTTGCTTTGCAAGAATTGCGCCCTTCTTTTTTTAAGGTGTCACCTTCAAATGAGGTCAAGTATGTTGGCTTACCAATCCCAAAAGAGATTTTAGAAAGTTCTATGGATCAAGGTATCTGTCATTCAGAACATAGTCCAATGAGAAAAATGTCAGGTGAGCAAGAATTTGCTTCTGTTGGTTCCAGTGCAAAGAAGCAAAAGATCAGCCAGAACACTAGATTAGTTAGGCAATGGCCTCAGTTCCCCACTAGTTCCCGTTTCCAACGTGAAACTAGGAATGCTAGCCACATCAACATCAATGGTCTGCAAAATAGAAGTCCTCATATGTATAATGCAGCTCAACAACACTTGACTTCAATCACTGAGCAATTGGAAGACAAGTGGTATATTAGTCCTGAAGAACTCAATGAAGGGAGCTGTACAGTTTTGTCAAATATCTACAGTCTGGGCGTTCTTTTTTTTGAGTTGCTGGTCCGTTTTGACTCAAATAGTGCCATTGTCTCAGCAATGTTCAATCTGCGTCATAGAATTCTTCCTCCGAATTTTCTGTCAGAATACGCTAAAGAGGCTGGATTTTGTCTTTGGTTACTTCATCCTGAACCGTCTTCACGTCCAACAACAAGGGAAATTCTACAATCAGAAGTAGTTAATGGATTACAGGAGATGTGTGAAGAGAAAGTGGCATCATCTGTGGTCGAAGAGGATGCTGAATTGGAACTATTATCCCATTTCCTCACGTCACTGAAAGAAAAGAAGCAGAAGGATGCCTCCAAGTTGATGGCAAGCATTAGGGTTTTAGAAGCAGATGCTGAAGAAGCTGAGAGGAGGCACTATTCAAGAAATCCCGACTGGTACAAAGAGTCCCTCGGTGGATGGAAAAACGCATGTAAAGAAGCTTCAAGATCAGAGGTGCTTTCTCCATTACCCCCTCTTCCTAGTTCAAATGAGTCAAGGTTAACGAGGAATATTGATCAGCTTGAGAGTGCTTACTTCTCTATGAGATCTATGATTCAGCCTCCTGAAGCTGATTGTAATATACGCATAGATAAAGAGTTGTTAAAATCTCGCAAGAATTGGTATTTGGAATCAGATGATGACGAAAAGAAGACTCCTACCGATCAATTGGGAGCTATCTTTGATGGTTTATGCAAGTATGATCGGTACAGTAACTTTGAAGTATGTGGAGTACTAAGAAATGGAGATTTCAACAGTAACTCAAATTTGATTTGCTCTTTGAGTTTTGATCGGGATGAGGACTACATTGCTTCTGCTGGGGTTTCAAAGAAAATAAAGATTTATGAGTTCAATGCACTTTTCAATGATTCTGTTGATATTCATTATCCATCGATCGAGATGTCAAATGAATCAAAGATTAGCTGTATTTGCTGGAACAACTACATAAAAAACTATCTCACTTCAACTGATTATGAAGGTGTAGTGAAGTTGTGGGATGTCAGCACTGGTCAAGAGTTTGCTCGATACAGAGGGCATGAACAAAAGGCTTGGTCCGTTGACTTCTCTCAAGTAGATCCTACAAAATTAGCCAGCGGGAGTGATGATGGTTCTGTGAAACTCTGGAGCATTAATGAGAAAAACTGTTTAGGCACCATCAGAAACAGAAACATTGCAAATGTATGCTGTGTACAGTTCTCTGCTCACTCCACTAATTTGCTGGCCTTTGGGTCTGCGGACTACAACACATATTGCTACGATCTTCGTAATGCTAGAACGCCCTGGTGTGTATTGGTTGGTCACCAAAAAAGCGTAAGCTATGTCAAATTCTTGGACTCTGAGACCCTTGTTTCTGCATCCACTGACAACACATTAAAGCTATGGGATCTCAACAAAACCATTGCCAGTGGTCCATCCACCAATGCTTGCAGCTTAACCCTCAGTGGGCATACCAATGAGAAGAACTTTGTGGGCTTATCCGTTTCTAATGGTTATATAGCTTGCGGTTCAGAAACAAATGAGGTCTATGCTTATTATAGATCTCTGCCTATGCCGATCACTTCATACAAATTTGGATCCATTGATCCTATAACCGGAAAGGAGACTGATGATATTAATGGATCATTTGTTTCAAGTGTCTGCTGGAGACAGAAATCAAACATGGTGGTTGCCGCCAATTCAAGCGGGTGCATAAAAGTGTTGCAGATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

947

Amino Acids

105.87

Weight (kDa)

5.71

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 346 - 440 4.7e-06 Protein kinase domain
Beta-prop_WDR3_1st PF25173 631 - 797 7.2e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 631 - 888 6.4e-32 WDR5 beta-propeller domain
WDR55 PF24796 633 - 764 6.2e-07 WDR55
Beta-prop_TEP1_2nd PF25047 634 - 753 8.1e-06 TEP-1 second beta-propeller
WD40_Gbeta PF25391 635 - 801 7.2e-09 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 635 - 841 1.9e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 637 - 756 2.3e-06 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 642 - 724 8.3e-08 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 646 - 838 5.6e-14 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 678 - 792 8.8e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 679 - 766 5.8e-08 Prp19 WD40 domain
Beta-prop_WDR19_1st PF23389 687 - 840 1.8e-06 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 695 - 842 4.6e-09 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 697 - 786 1.9e-07 EIF3I
Beta-prop_EML_2 PF23414 698 - 843 4e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 706 - 763 9.9e-06 WDHD1 first WD40 domain
WD40 PF00400 718 - 753 7e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 727 - 843 8.4e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 738 - 888 1e-19 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 740 - 842 1.2e-10 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 766 - 942 5.3e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 769 - 881 2.2e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 771 - 945 2.2e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 601
AccB1I GGYRCC 1 cut(s) 2282
AccI GTMKAC 2 cut(s) 163, 371
AccIII TCCGGA 1 cut(s) 324
AciI CCGC 7 cut(s) 269, 411, 2226, 2366, 2634, 2802, 2814
AclWI GGATC 8 cut(s) 666, 792, 2201, 2523, 2700, 2708, 2713, 2758
AcsI RAATTY 8 cut(s) 356, 842, 1206, 1219, 1305, 1897, 2456, 2699
AcuI CTGAAG 4 cut(s) 44, 1095, 1509, 1719
AcyI GRCGYC 1 cut(s) 598
AfaI GTAC 8 cut(s) 130, 206, 442, 1099, 1532, 1841, 1866, 2322
AfiI CCNNNNNNNGG 3 cut(s) 214, 2574, 2723
AflII CTTAAG 1 cut(s) 574
AflIII ACRYGT 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 133
AhlI ACTAGT 1 cut(s) 926
AjiI CACGTC 2 cut(s) 1289, 1416
AjnI CCWGG 1 cut(s) 2411
AjuI GAANNNNNNNTTGG 2 cut(s) 1742, 1774
Alw21I GWGCWC 2 cut(s) 55, 194
Alw26I GTCTC 5 cut(s) 82, 1183, 2465, 2726, 2770
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 8 cut(s) 666, 792, 2201, 2523, 2700, 2708, 2713, 2758
AlwNI CAGNNNCTG 1 cut(s) 1487
Aor13HI TCCGGA 1 cut(s) 324
AoxI GGCC 3 cut(s) 236, 911, 2352
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 1013, 2559
ApoI RAATTY 8 cut(s) 356, 842, 1206, 1219, 1305, 1897, 2456, 2699
ArsI GACNNNNNNTTYG 6 cut(s) 92, 124, 979, 1011, 1211, 1243
AseI ATTAAT 2 cut(s) 2262, 2745
AsiGI ACCGGT 1 cut(s) 133
AspLEI GCGC 1 cut(s) 699
AspS9I GGNCC 3 cut(s) 1150, 2184, 2540
AsuHPI GGTGA 5 cut(s) 152, 416, 711, 845, 2423
AsuNHI GCTAGC 1 cut(s) 959
AvaII GGWCC 3 cut(s) 1150, 2184, 2540
BaeGI GKGCMC 2 cut(s) 55, 540
BamHI GGATCC 1 cut(s) 2705
BanI GGYRCC 1 cut(s) 2282
BanII GRGCYC 1 cut(s) 194
BarI GAAGNNNNNNTAC 2 cut(s) 1558, 1590
BauI CACGAG 1 cut(s) 653
BbsI GAAGAC 3 cut(s) 1059, 1275, 1790
Bbv12I GWGCWC 2 cut(s) 55, 194
BbvI GCAGC 2 cut(s) 1025, 2571
BccI CCATC 9 cut(s) 284, 401, 1450, 1545, 1811, 2029, 2231, 2294, 2551
BcgI CGANNNNNNTGC 2 cut(s) 1364, 1398
BciT130I CCWGG 1 cut(s) 2413
BciVI GTATCC 1 cut(s) 332
BclI TGATCA 1 cut(s) 1648
BcoDI GTCTC 5 cut(s) 82, 1183, 2465, 2726, 2770
BcuI ACTAGT 1 cut(s) 926
BfaI CTAG 6 cut(s) 894, 927, 951, 960, 1614, 2403
BfmI CTRYAG 2 cut(s) 285, 1117
BfrI CTTAAG 1 cut(s) 574
BfuI GTATCC 1 cut(s) 332
BglII AGATCT 2 cut(s) 1679, 2669
BisI GCNGC 3 cut(s) 1014, 2560, 2802
BlsI GCNGC 3 cut(s) 1015, 2561, 2803
BmcAI AGTACT 1 cut(s) 1866
Bme1390I CCNGG 1 cut(s) 2413
Bme18I GGWCC 3 cut(s) 1150, 2184, 2540
BmgBI CACGTC 2 cut(s) 1289, 1416
BmgT120I GGNCC 3 cut(s) 1150, 2184, 2540
BmiI GGNNCC 3 cut(s) 859, 2284, 2707
BmrFI CCNGG 1 cut(s) 2413
BmsI GCATC 7 cut(s) 114, 484, 1370, 1372, 1432, 1474, 2495
BmtI GCTAGC 1 cut(s) 963
BpiI GAAGAC 3 cut(s) 1059, 1275, 1790
BpmI CTGGAG 2 cut(s) 2275, 2794
BpuEI CTTGAG 4 cut(s) 178, 657, 1676, 2184
Bsa29I ATCGAT 1 cut(s) 2024
BsaHI GRCGYC 1 cut(s) 598
BsaI GGTCTC 1 cut(s) 2465
BsaJI CCNNGG 4 cut(s) 45, 552, 1543, 2411
BsaWI WCCGGW 3 cut(s) 133, 324, 2723
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 2574, 2723
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 6 cut(s) 167, 457, 861, 1532, 2143, 2535
Bse3DI GCAATG 5 cut(s) 914, 1191, 1938, 2301, 2529
BseAI TCCGGA 1 cut(s) 324
BseBI CCWGG 1 cut(s) 2413
BseCI ATCGAT 1 cut(s) 2024
BseDI CCNNGG 4 cut(s) 45, 552, 1543, 2411
BseLI CCNNNNNNNGG 3 cut(s) 214, 2574, 2723
BseMI GCAATG 5 cut(s) 914, 1191, 1938, 2301, 2529
BseMII CTCAG 9 cut(s) 177, 245, 578, 929, 1032, 1194, 1488, 2460, 2584
BseNI ACTGG 6 cut(s) 167, 457, 861, 1532, 2143, 2535
BseRI GAGGAG 1 cut(s) 1516
BseSI GKGCMC 2 cut(s) 55, 540
BseXI GCAGC 2 cut(s) 1025, 2571
BseYI CCCAGC 1 cut(s) 1949
Bsh1285I CGRYCG 2 cut(s) 45, 2028
BshFI GGCC 3 cut(s) 238, 913, 2354
BshNI GGYRCC 1 cut(s) 2282
BshTI ACCGGT 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 2024
BsiEI CGRYCG 2 cut(s) 45, 2028
BsiHKAI GWGCWC 2 cut(s) 55, 194
BsiSI CCGG 3 cut(s) 134, 325, 2724
BslFI GGGAC 1 cut(s) 1525
BslI CCNNNNNNNGG 3 cut(s) 214, 2574, 2723
BsmAI GTCTC 5 cut(s) 82, 1183, 2465, 2726, 2770
BsmFI GGGAC 1 cut(s) 1525
BsmI GAATGC 2 cut(s) 13, 961
BsnI GGCC 3 cut(s) 238, 913, 2354
Bso31I GGTCTC 1 cut(s) 2465
Bsp1286I GDGCHC 3 cut(s) 55, 194, 540
Bsp13I TCCGGA 1 cut(s) 324
Bsp1407I TGTACA 2 cut(s) 1097, 2320
BspACI CCGC 7 cut(s) 269, 411, 2226, 2366, 2634, 2802, 2814
BspANI GGCC 3 cut(s) 238, 913, 2354
BspCNI CTCAG 9 cut(s) 178, 244, 579, 928, 1033, 1193, 1489, 2461, 2583
BspDI ATCGAT 1 cut(s) 2024
BspEI TCCGGA 1 cut(s) 324
BspLI GGNNCC 3 cut(s) 859, 2284, 2707
BspOI GCTAGC 1 cut(s) 963
BspPI GGATC 8 cut(s) 666, 792, 2201, 2523, 2700, 2708, 2713, 2758
BspT107I GGYRCC 1 cut(s) 2282
BspTI CTTAAG 1 cut(s) 574
BspTNI GGTCTC 1 cut(s) 2465
BsrDI GCAATG 5 cut(s) 914, 1191, 1938, 2301, 2529
BsrFI RCCGGY 1 cut(s) 133
BsrGI TGTACA 2 cut(s) 1097, 2320
BsrI ACTGG 6 cut(s) 167, 457, 861, 1532, 2143, 2535
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 4 cut(s) 45, 552, 1543, 2411
BssNAI GTATAC 1 cut(s) 164
BssNI GRCGYC 1 cut(s) 598
BssSI CACGAG 1 cut(s) 653
BssT1I CCWWGG 1 cut(s) 552
Bst1107I GTATAC 1 cut(s) 164
Bst2BI CACGAG 1 cut(s) 653
Bst2UI CCWGG 1 cut(s) 2413
Bst4CI ACNGT 9 cut(s) 286, 440, 1102, 1121, 1281, 1844, 1889, 2276, 2325
Bst6I CTCTTC 3 cut(s) 1344, 1371, 1614
BstACI GRCGYC 1 cut(s) 598
BstAFI CTTAAG 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 694
BstAUI TGTACA 2 cut(s) 1097, 2320
BstC8I GCNNGC 9 cut(s) 68, 77, 81, 961, 1462, 2224, 2352, 2557, 2632
BstEII GGTNACC 1 cut(s) 2429
BstHHI GCGC 1 cut(s) 699
BstMAI GTCTC 5 cut(s) 82, 1183, 2465, 2726, 2770
BstMCI CGRYCG 2 cut(s) 45, 2028
BstMWI GCNNNNNNNGC 7 cut(s) 72, 76, 303, 694, 1244, 1493, 2061
BstNI CCWGG 1 cut(s) 2413
BstNSI RCATGY 3 cut(s) 83, 109, 1566
BstPI GGTNACC 1 cut(s) 2429
BstSCI CCNGG 1 cut(s) 2411
BstSFI CTRYAG 2 cut(s) 285, 1117
BstSLI GKGCMC 2 cut(s) 55, 540
BstV1I GCAGC 2 cut(s) 1025, 2571
BstV2I GAAGAC 3 cut(s) 1059, 1275, 1790
BstX2I RGATCY 5 cut(s) 1679, 2206, 2515, 2669, 2705
BstXI CCANNNNNNTGG 1 cut(s) 1456
BstYI RGATCY 5 cut(s) 1679, 2206, 2515, 2669, 2705
BstZ17I GTATAC 1 cut(s) 164
Bsu15I ATCGAT 1 cut(s) 2024
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 3 cut(s) 238, 913, 2354
BsuTUI ATCGAT 1 cut(s) 2024
BtrI CACGTC 2 cut(s) 1289, 1416
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 8 cut(s) 18, 868, 1038, 1418, 2136, 2490, 2542, 2578
Cac8I GCNNGC 9 cut(s) 68, 77, 81, 961, 1462, 2224, 2352, 2557, 2632
CaiI CAGNNNCTG 1 cut(s) 1487
CfoI GCGC 1 cut(s) 699
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 1150, 2184, 2540
ClaI ATCGAT 1 cut(s) 2024
CseI GACGC 1 cut(s) 1187
Csp6I GTAC 8 cut(s) 129, 205, 441, 1098, 1531, 1840, 1865, 2321
CspAI ACCGGT 1 cut(s) 133
CspCI CAANNNNNGTGG 6 cut(s) 534, 569, 636, 671, 2775, 2810
CviAII CATG 9 cut(s) 80, 92, 106, 210, 416, 560, 1563, 2168, 2791
CviQI GTAC 8 cut(s) 129, 205, 441, 1098, 1531, 1840, 1865, 2321
Eam1104I CTCTTC 3 cut(s) 1344, 1371, 1614
EarI CTCTTC 3 cut(s) 1344, 1371, 1614
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 2465
Eco47I GGWCC 3 cut(s) 1150, 2184, 2540
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 4 cut(s) 44, 1095, 1509, 1719
Eco91I GGTNACC 1 cut(s) 2429
EcoICRI GAGCTC 1 cut(s) 192
EcoO65I GGTNACC 1 cut(s) 2429
EcoRI GAATTC 1 cut(s) 1206
EcoRII CCWGG 1 cut(s) 2411
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 9 cut(s) 83, 95, 109, 213, 419, 563, 1566, 2171, 2794
FaqI GGGAC 1 cut(s) 1525
FatI CATG 9 cut(s) 79, 91, 105, 209, 415, 559, 1562, 2167, 2790
FauI CCCGC 2 cut(s) 2219, 2807
FauNDI CATATG 2 cut(s) 313, 1002
FbaI TGATCA 1 cut(s) 1648
FblI GTMKAC 2 cut(s) 163, 371
Fnu4HI GCNGC 3 cut(s) 1014, 2560, 2802
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 1014, 2560, 2802
FspBI CTAG 6 cut(s) 894, 927, 951, 960, 1614, 2403
GlaI GCGC 1 cut(s) 698
GluI GCNGC 3 cut(s) 1014, 2560, 2802
GsaI CCCAGC 1 cut(s) 1953
GsuI CTGGAG 2 cut(s) 2275, 2794
HaeIII GGCC 3 cut(s) 238, 913, 2354
HapII CCGG 3 cut(s) 134, 325, 2724
HgaI GACGC 1 cut(s) 1187
HhaI GCGC 1 cut(s) 699
Hin1I GRCGYC 1 cut(s) 598
Hin1II CATG 9 cut(s) 83, 95, 109, 213, 419, 563, 1566, 2171, 2794
Hin6I GCGC 1 cut(s) 697
HinP1I GCGC 1 cut(s) 697
HincII GTYRAC 2 cut(s) 1635, 2191
HindII GTYRAC 2 cut(s) 1635, 2191
HindIII AAGCTT 1 cut(s) 1572
HpaI GTTAAC 1 cut(s) 1635
HpaII CCGG 3 cut(s) 134, 325, 2724
HphI GGTGA 5 cut(s) 152, 416, 711, 845, 2423
Hpy166II GTNNAC 7 cut(s) 53, 131, 164, 372, 1635, 2191, 2321
Hpy8I GTNNAC 7 cut(s) 53, 131, 164, 372, 1635, 2191, 2321
HpyAV CCTTC 7 cut(s) 373, 522, 711, 732, 1082, 1432, 2102
HpyCH4III ACNGT 9 cut(s) 286, 440, 1102, 1121, 1281, 1844, 1889, 2276, 2325
HpyCH4IV ACGT 4 cut(s) 598, 943, 1288, 1415
HpyF10VI GCNNNNNNNGC 7 cut(s) 72, 76, 303, 694, 1244, 1493, 2061
HpySE526I ACGT 4 cut(s) 598, 943, 1288, 1415
Hsp92I GRCGYC 1 cut(s) 598
Hsp92II CATG 9 cut(s) 83, 95, 109, 213, 419, 563, 1566, 2171, 2794
HspAI GCGC 1 cut(s) 697
Kpn2I TCCGGA 1 cut(s) 324
Ksp22I TGATCA 1 cut(s) 1648
KspAI GTTAAC 1 cut(s) 1635
LmnI GCTCC 3 cut(s) 1092, 1805, 2256
Lsp1109I GCAGC 2 cut(s) 1025, 2571
LweI GCATC 7 cut(s) 114, 484, 1370, 1372, 1432, 1474, 2495
MaeI CTAG 6 cut(s) 894, 927, 951, 960, 1614, 2403
MaeII ACGT 4 cut(s) 598, 943, 1288, 1415
MaeIII GTNAC 8 cut(s) 140, 448, 717, 1263, 1416, 1844, 1889, 2429
MfeI CAATTG 3 cut(s) 178, 1046, 1799
MflI RGATCY 5 cut(s) 1679, 2206, 2515, 2669, 2705
MhlI GDGCHC 3 cut(s) 55, 194, 540
MlyI GAGTC 5 cut(s) 1154, 1547, 1634, 1780, 2459
MmeI TCCRAC 3 cut(s) 151, 964, 1316
Mph1103I ATGCAT 1 cut(s) 85
MroI TCCGGA 1 cut(s) 324
MslI CAYNNNNRTG 3 cut(s) 96, 2583, 2825
MspA1I CMGCKG 1 cut(s) 2226
MspCI CTTAAG 1 cut(s) 574
MspI CCGG 3 cut(s) 134, 325, 2724
MspR9I CCNGG 1 cut(s) 2413
MunI CAATTG 3 cut(s) 178, 1046, 1799
Mva1269I GAATGC 2 cut(s) 13, 961
MvaI CCWGG 1 cut(s) 2413
MwoI GCNNNNNNNGC 7 cut(s) 72, 76, 303, 694, 1244, 1493, 2061
NdeI CATATG 2 cut(s) 313, 1002
NheI GCTAGC 1 cut(s) 959
NlaIII CATG 9 cut(s) 83, 95, 109, 213, 419, 563, 1566, 2171, 2794
NlaIV GGNNCC 3 cut(s) 859, 2284, 2707
NmuCI GTSAC 4 cut(s) 140, 717, 1416, 2429
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 3 cut(s) 83, 109, 1566
PaeI GCATGC 1 cut(s) 83
PciI ACATGT 1 cut(s) 105
PctI GAATGC 2 cut(s) 13, 961
PfeI GAWTC 6 cut(s) 422, 476, 1687, 1763, 2000, 2042
PinAI ACCGGT 1 cut(s) 133
PkrI GCNGC 3 cut(s) 1015, 2561, 2803
Ple19I CGATCG 1 cut(s) 2028
PleI GAGTC 5 cut(s) 1154, 1546, 1633, 1780, 2459
PpsI GAGTC 5 cut(s) 1154, 1546, 1633, 1780, 2459
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 2 cut(s) 2262, 2745
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 2411
PspEI GGTNACC 1 cut(s) 2429
PspFI CCCAGC 1 cut(s) 1949
PspGI CCWGG 1 cut(s) 2411
PspN4I GGNNCC 3 cut(s) 859, 2284, 2707
PspPI GGNCC 3 cut(s) 1150, 2184, 2540
PstNI CAGNNNCTG 1 cut(s) 1487
PsuI RGATCY 5 cut(s) 1679, 2206, 2515, 2669, 2705
PvuI CGATCG 1 cut(s) 2028
RsaI GTAC 8 cut(s) 130, 206, 442, 1099, 1532, 1841, 1866, 2322
RsaNI GTAC 8 cut(s) 129, 205, 441, 1098, 1531, 1840, 1865, 2321
RseI CAYNNNNRTG 3 cut(s) 96, 2583, 2825
SacI GAGCTC 1 cut(s) 194
SatI GCNGC 3 cut(s) 1014, 2560, 2802
Sau96I GGNCC 3 cut(s) 1150, 2184, 2540
ScaI AGTACT 1 cut(s) 1866
SchI GAGTC 5 cut(s) 1154, 1547, 1634, 1780, 2459
ScrFI CCNGG 1 cut(s) 2413
SduI GDGCHC 3 cut(s) 55, 194, 540
SfaNI GCATC 7 cut(s) 114, 484, 1370, 1372, 1432, 1474, 2495
SfcI CTRYAG 2 cut(s) 285, 1117
SinI GGWCC 3 cut(s) 1150, 2184, 2540
SmiMI CAYNNNNRTG 3 cut(s) 96, 2583, 2825
SmlI CTYRAG 5 cut(s) 193, 574, 672, 1655, 2199
SmoI CTYRAG 5 cut(s) 193, 574, 672, 1655, 2199
SpeI ACTAGT 1 cut(s) 926
SphI GCATGC 1 cut(s) 83
SsiI CCGC 7 cut(s) 269, 411, 2226, 2366, 2634, 2802, 2814
SspI AATATT 1 cut(s) 1645
SspMI CTAG 6 cut(s) 894, 927, 951, 960, 1614, 2403
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 2411
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 9 cut(s) 286, 440, 1102, 1121, 1281, 1844, 1889, 2276, 2325
TaiI ACGT 4 cut(s) 601, 946, 1291, 1418
TaqI TCGA 6 cut(s) 41, 111, 1374, 2024, 2028, 2155
TaqII GACCGA 1 cut(s) 59
TatI WGTACW 4 cut(s) 440, 1097, 1864, 2320
TauI GCSGC 1 cut(s) 2804
TfiI GAWTC 6 cut(s) 422, 476, 1687, 1763, 2000, 2042
TscAI CASTG 8 cut(s) 18, 868, 1045, 1425, 2143, 2497, 2542, 2578
TseFI GTSAC 4 cut(s) 140, 717, 1416, 2429
TseI GCWGC 2 cut(s) 1013, 2559
Tsp45I GTSAC 4 cut(s) 140, 717, 1416, 2429
TspDTI ATGAA 8 cut(s) 300, 1101, 1259, 2003, 2055, 2121, 2184, 2682
TspGWI ACGGA 3 cut(s) 1142, 2176, 2599
TspRI CASTG 8 cut(s) 18, 868, 1045, 1425, 2143, 2497, 2542, 2578
Vha464I CTTAAG 1 cut(s) 574
VneI GTGCAC 1 cut(s) 51
VpaK11BI GGWCC 3 cut(s) 1150, 2184, 2540
VspI ATTAAT 2 cut(s) 2262, 2745
XapI RAATTY 8 cut(s) 356, 842, 1206, 1219, 1305, 1897, 2456, 2699
XceI RCATGY 3 cut(s) 83, 109, 1566
XcmI CCANNNNNNNNNTGG 1 cut(s) 2347
XmiI GTMKAC 2 cut(s) 163, 371
XspI CTAG 6 cut(s) 894, 927, 951, 960, 1614, 2403
ZraI GACGTC 1 cut(s) 599
ZrmI AGTACT 1 cut(s) 1866
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.