Rroxscaffold_3G00260410

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012794
Physical Location & Seq
Reverse (-)
54826025 .. 54832753
6729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260410.1

Sequence Viewer

Length: 2979 bp
ATGCAGTGGGTGGGGAAGCCACCCCACCTAATTCGACGGAGGGTGCAGCTTCAGAGCAAGGAGAACGAGCATGCATTGAGACATGGAAATGACCACATGTTCGACTACCAAGAGATGCGTATACCGGGTGGTGACGAAGATCATTCGCTGCGAATACCAGTTGGAAGCAGTTGTCCTGAGAGCTCAAGTCAGGAGCGTTTTGTAAAACCGTATGATGGCTTAGGCTTATATCCAAGCGGATCAAGGAGCGGGAATGGTGGGAGTTCACGAGTCAATACTGCATTCAGAGATTGTGGTCAGATGAGATCAAATGATTTGGAGAAAGTGGAGTACCCATCTCTGGATGACATTATCGCTCAGAGGGCAAATAGTGATAATCTGGTTGAAGTTGTGGCCGAATTGACAAATACTGTAGATGGAGGGGTTTCAGGCAATTCATATGGAGGTATCCGGACAAAAATGCTTTCCAAATCAAGATCAGAATTTTATGTTAAGTCTACATTGAAGGGTAAGGGTGTTATTTTCAAAGGTCCAAATCATGATGGCGGTCATGTTGAATCTAAGAACCTTAACAGTACAAAGGTTACTGGTGGTTCTAAGGCTACTTCTGATTCAATACAGAGTTTGGATGCCAAAATTGTTATGCCTTCTTCCAATGATGTGAACGTTGTGCCCAAACCACCAGCCTTGGAACATGATGAAATCAGTTTAAGAGAATGGCTGAGAATTGGACGTCCTAAAGTCAATAAAGTTGAGTGTCTGTATGTATTCAAACAAATTGCAAATCTCGTGGATCATTTTCACTCTCAAGGAGTTGCTTTGCAAGAATTGCGCCCTTCTTTTTTTAAGGTGTCACCTTCAAATGAGGTCAAGTATGTTGGCTTACCAATCCCAAAAGAGATATTAGAGAGTTCTATGGATCAAGGTATCTGTCATTCAGAAAATAGTCCAATGAGAAAAATGTCAGGTGAGCAAGAATTTGCTTGTGTTGGTTCCAGTGCAAAGAAGCAAAAGATCAGCCAGAACACTAGATTAGTTAGGCAATGGCCTCAGTTCCCCACTAGTTCCCGTTTCCAACGTGAAACTAGGAATACTAGCCACATCAACATCAGTGGTCTGCAAAATAGAAGTCCTCATATGTGTAATGCAGCTCAACAACACTTGACTTCAATCACTGAGCAATTGGAAGAAAAGTGGTATATTAGTCCTGAAGAACTCAGTGAAGGGAGCTGTACAGTTTTGTCAAATATCTACAGTCTGGGCGTTCTTCTTTTTGAGTTGCTGGCCCGTTTTGACTCAAATAGTGCCGTTGTCTCAGCAATGTTCAATCTGCGTCATAGAATTCTTCCTCCAAATTTTCTGTCAGAAAACGCGAAAGATGCTGGATTTTGTCTTTGGTTACTTCATCCTGAACCGTCTTCACGTCCAACAACAAGGGAAATTCTACAATCAGAAGTAGTTAATGGATTACAGGAGATGTGTGAAGAGAAAGTGGCATCATCTGTGGTCGAAGAGGATGCTGAATTGGAACTATTATCCCATTTCCTCACGTCACTGAAAGAAAAGAAGCAGAAGGATGCCTCCAAGTTGATGGCAAGCATTAGGGTTTTGGAAGCAGATGCTGAAGAAGCTGAGAGGAGGCACTATTCAAGAAATCCCGACTGGTACAAAGAGTCCCTCGGTGGATGGAAAATCGCATGTAAAGAAGCTTCAAGATCAGAGGTGCTTTCTCCATTACCCCCTCTTCCTAGTTCAAATGAGTCAAGGTTGACGAGGAATATTGATCAGCTTGAGAGCGCTTACTTCTCTATGAGATCTAGGATTCAGCTTCCTGAAGCTGATTGTACTATACGCATAGATAAAGAGTTATTAAAATCTCGCAAGAATTGGTATTTGGAATCAGATGATGATGAAAAGAAGACTCCTACCTATCAATTGGGAGCTATCTTTGATGGTTTATGCAAGTATGATCGGTACAGTAAGTTTGAAGTATGTGGAGTGCTAAGAAATGGAGATTTCAACAGTAACTCAAATTTGATTTGCTCTTTGAGTTTTGATCGGGATGAGGACTACATCGCTTCTGCTGGGGTTTCAAAGAAAATAAAGATTTATGAGTTCAATGCACTTTTCAATGATTCTGTTGATATTCATTATCCATCGATCGAGATGTCAAATGAATCAAAGATTAGCTGTATTTGCTGGAACAACTACATAAAAAACTATCTCACTTCAACTGATTATGAAGGTGTAGTGAAGTTGTGGGATGTCAGCACTAGTCAAGAGTTTGCTCGATACAGAGGGCATGAACAAAAGGTTTGGTCTGTTGACTTCTCTCAAGTAGATCCTACAAAATTAGCCAGCGGGAGTGATGATGGTTCTGTGAAACTCTGGAGCATTAATGAGAAAAACTGTTTAGGCACCATCAGAAACAGAAACATTGCAAATGTGTGCTGTGTACAGTTCTCTGCTCACTCCACTAATTTGCTGGCCTTTGGGTCTGCGGACTACAACACATATTGCTATGATCTTCGTAATGCTAGAACGCCCTGGTGTGTATTGGTTGGTCACCAAAAAAGCGTAAGCTATGTCAAATTCTTGGACTCTGAGACCCTTGTTTCTGCATCCACTGACAACACATTAAAGCTATGGGATCTCAACAAAACCACTGCCAGTGGTCCATCCACCAATGCTTGCAGCTTAACCCTCTGTGGGCATACCAATGAGAAGAACTTTGTGGGCCTATCCGTTTCTAATGGTTATATAGCATGCGGTTCAGAAACAAATGAGGTCTATGCTTATTACAGATCTCTGCCTATGCCGATGACTTCATACAAATTTGGCTCCATTGATCCCATAACCGGAAAGGAGACTGATGATATTAATGGATCATTTGTTTCAAGTGTCTGCTGGAGACAGAAATCAAACATGGTGGTTGCCGCCAATTCAAGCGGGTGCATAAAAGTGTTGCAGATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

992

Amino Acids

111.12

Weight (kDa)

6.33

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 391 - 485 1.6e-06 Protein kinase domain
Beta-prop_WDR5 PF25175 676 - 933 3.1e-32 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 676 - 810 7.3e-14 WDR3 first beta-propeller domain
WDR55 PF24796 678 - 808 1.9e-06 WDR55
Beta-prop_TEP1_2nd PF25047 679 - 798 1.5e-06 TEP-1 second beta-propeller
Beta-prop_EML PF23409 680 - 888 7.3e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 680 - 846 1.5e-08 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 681 - 836 4.8e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 682 - 801 4.5e-07 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 687 - 777 3.9e-08 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 691 - 883 7.8e-15 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 724 - 811 5.6e-09 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 743 - 889 2.9e-12 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 750 - 831 4.7e-07 EIF3I
WD40_WDHD1_1st PF24817 751 - 808 5e-07 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 760 - 888 2.4e-09 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 763 - 798 2.1e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 770 - 890 2e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 783 - 933 9.2e-20 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 785 - 888 1.2e-10 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 811 - 890 8.9e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 814 - 926 6.9e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 816 - 990 5.3e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 736
AccB1I GGYRCC 1 cut(s) 2417
AccBSI CCGCTC 1 cut(s) 249
AccI GTMKAC 2 cut(s) 121, 497
AccII CGCG 1 cut(s) 1373
AccIII TCCGGA 1 cut(s) 450
AciI CCGC 8 cut(s) 237, 249, 546, 2361, 2501, 2769, 2937, 2949
AclI AACGTT 1 cut(s) 666
AclWI GGATC 7 cut(s) 247, 801, 927, 2336, 2658, 2843, 2893
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 8 cut(s) 482, 977, 1341, 1354, 1440, 2032, 2591, 2834
AcuI CTGAAG 4 cut(s) 35, 1230, 1644, 1854
AcyI GRCGYC 1 cut(s) 733
AfaI GTAC 7 cut(s) 332, 577, 1234, 1667, 1846, 1976, 2457
AfeI AGCGCT 1 cut(s) 1798
AfiI CCNNNNNNNGG 4 cut(s) 215, 340, 2709, 2858
AflIII ACRYGT 1 cut(s) 96
AhlI ACTAGT 2 cut(s) 1061, 2273
AjiI CACGTC 2 cut(s) 1424, 1551
AjnI CCWGG 1 cut(s) 2546
AjuI GAANNNNNNNTTGG 2 cut(s) 1877, 1909
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 5 cut(s) 73, 1318, 2600, 2861, 2905
AlwI GGATC 7 cut(s) 247, 801, 927, 2336, 2658, 2843, 2893
AlwNI CAGNNNCTG 1 cut(s) 1622
Aor13HI TCCGGA 1 cut(s) 450
Aor51HI AGCGCT 1 cut(s) 1798
AoxI GGCC 5 cut(s) 393, 1046, 1284, 2487, 2737
ApeKI GCWGC 4 cut(s) 46, 148, 1148, 2694
ApoI RAATTY 8 cut(s) 482, 977, 1341, 1354, 1440, 2032, 2591, 2834
ArsI GACNNNNNNTTYG 6 cut(s) 83, 115, 1114, 1146, 1346, 1378
AseI ATTAAT 2 cut(s) 2397, 2880
AspLEI GCGC 2 cut(s) 834, 1799
AspS9I GGNCC 4 cut(s) 530, 1285, 2675, 2737
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 4 cut(s) 143, 846, 980, 2558
AvaII GGWCC 2 cut(s) 530, 2675
BaeGI GKGCMC 1 cut(s) 675
BanI GGYRCC 1 cut(s) 2417
BanII GRGCYC 1 cut(s) 185
BarI GAAGNNNNNNTAC 2 cut(s) 1693, 1725
BauI CACGAG 2 cut(s) 267, 788
BbsI GAAGAC 2 cut(s) 1410, 1925
Bbv12I GWGCWC 1 cut(s) 185
BbvI GCAGC 4 cut(s) 58, 135, 1160, 2706
BceAI ACGGC 1 cut(s) 1292
BcgI CGANNNNNNTGC 2 cut(s) 1499, 1533
BciT130I CCWGG 1 cut(s) 2548
BciVI GTATCC 1 cut(s) 458
BclI TGATCA 1 cut(s) 1783
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 5 cut(s) 73, 1318, 2600, 2861, 2905
BcuI ACTAGT 2 cut(s) 1061, 2273
BfaI CTAG 8 cut(s) 1029, 1062, 1086, 1095, 1749, 1818, 2274, 2538
BfmI CTRYAG 2 cut(s) 411, 1252
BfoI RGCGCY 1 cut(s) 1800
BfuI GTATCC 1 cut(s) 458
BglII AGATCT 2 cut(s) 1814, 2804
BisI GCNGC 5 cut(s) 47, 149, 1149, 2695, 2937
BlsI GCNGC 5 cut(s) 48, 150, 1150, 2696, 2938
Bme1390I CCNGG 2 cut(s) 126, 2548
Bme18I GGWCC 2 cut(s) 530, 2675
BmgBI CACGTC 2 cut(s) 1424, 1551
BmgT120I GGNCC 4 cut(s) 530, 1285, 2675, 2737
BmiI GGNNCC 3 cut(s) 994, 2419, 2842
BmrFI CCNGG 2 cut(s) 126, 2548
BmsI GCATC 8 cut(s) 105, 619, 1369, 1505, 1507, 1567, 1609, 2630
BpiI GAAGAC 2 cut(s) 1410, 1925
BpmI CTGGAG 2 cut(s) 2410, 2929
Bpu10I CCTNAGC 1 cut(s) 220
BpuEI CTTGAG 4 cut(s) 169, 792, 1811, 2319
BpuMI CCSGG 1 cut(s) 126
Bsa29I ATCGAT 1 cut(s) 2159
BsaHI GRCGYC 1 cut(s) 733
BsaI GGTCTC 1 cut(s) 2600
BsaJI CCNNGG 3 cut(s) 687, 1678, 2546
BsaWI WCCGGW 2 cut(s) 450, 2858
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 340, 2709, 2858
Bse1I ACTGG 5 cut(s) 158, 592, 996, 1667, 2670
Bse3DI GCAATG 3 cut(s) 1049, 1326, 2436
BseAI TCCGGA 1 cut(s) 450
BseBI CCWGG 1 cut(s) 2548
BseCI ATCGAT 1 cut(s) 2159
BseDI CCNNGG 3 cut(s) 687, 1678, 2546
BseLI CCNNNNNNNGG 4 cut(s) 215, 340, 2709, 2858
BseMI GCAATG 3 cut(s) 1049, 1326, 2436
BseMII CTCAG 9 cut(s) 168, 371, 713, 1064, 1167, 1231, 1329, 1623, 2595
BseNI ACTGG 5 cut(s) 158, 592, 996, 1667, 2670
BseRI GAGGAG 1 cut(s) 1651
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 4 cut(s) 58, 135, 1160, 2706
BseYI CCCAGC 1 cut(s) 2084
BsgI GTGCAG 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 1373
Bsh1285I CGRYCG 1 cut(s) 2163
BshFI GGCC 5 cut(s) 395, 1048, 1286, 2489, 2739
BshNI GGYRCC 1 cut(s) 2417
BshVI ATCGAT 1 cut(s) 2159
BsiEI CGRYCG 1 cut(s) 2163
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 3 cut(s) 125, 451, 2859
BslFI GGGAC 1 cut(s) 1660
BslI CCNNNNNNNGG 4 cut(s) 215, 340, 2709, 2858
BsmAI GTCTC 5 cut(s) 73, 1318, 2600, 2861, 2905
BsmFI GGGAC 1 cut(s) 1660
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 5 cut(s) 395, 1048, 1286, 2489, 2739
Bso31I GGTCTC 1 cut(s) 2600
Bsp1286I GDGCHC 2 cut(s) 185, 675
Bsp13I TCCGGA 1 cut(s) 450
Bsp1407I TGTACA 2 cut(s) 1232, 2455
BspACI CCGC 8 cut(s) 237, 249, 546, 2361, 2501, 2769, 2937, 2949
BspANI GGCC 5 cut(s) 395, 1048, 1286, 2489, 2739
BspCNI CTCAG 9 cut(s) 169, 370, 714, 1063, 1168, 1230, 1328, 1624, 2596
BspDI ATCGAT 1 cut(s) 2159
BspEI TCCGGA 1 cut(s) 450
BspFNI CGCG 1 cut(s) 1373
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 3 cut(s) 994, 2419, 2842
BspPI GGATC 7 cut(s) 247, 801, 927, 2336, 2658, 2843, 2893
BspT107I GGYRCC 1 cut(s) 2417
BspTNI GGTCTC 1 cut(s) 2600
BsrBI CCGCTC 1 cut(s) 249
BsrDI GCAATG 3 cut(s) 1049, 1326, 2436
BsrGI TGTACA 2 cut(s) 1232, 2455
BsrI ACTGG 5 cut(s) 158, 592, 996, 1667, 2670
BssECI CCNNGG 3 cut(s) 687, 1678, 2546
BssNAI GTATAC 1 cut(s) 122
BssNI GRCGYC 1 cut(s) 733
BssSI CACGAG 2 cut(s) 267, 788
BssT1I CCWWGG 1 cut(s) 687
Bst1107I GTATAC 1 cut(s) 122
Bst2BI CACGAG 2 cut(s) 267, 788
Bst2UI CCWGG 1 cut(s) 2548
Bst6I CTCTTC 3 cut(s) 1479, 1506, 1749
BstACI GRCGYC 1 cut(s) 733
BstAPI GCANNNNNTGC 1 cut(s) 829
BstAUI TGTACA 2 cut(s) 1232, 2455
BstC8I GCNNGC 7 cut(s) 72, 1284, 1597, 2359, 2487, 2692, 2767
BstEII GGTNACC 1 cut(s) 2564
BstFNI CGCG 1 cut(s) 1373
BstH2I RGCGCY 1 cut(s) 1800
BstHHI GCGC 2 cut(s) 834, 1799
BstMAI GTCTC 5 cut(s) 73, 1318, 2600, 2861, 2905
BstMCI CGRYCG 1 cut(s) 2163
BstMWI GCNNNNNNNGC 5 cut(s) 362, 829, 1379, 1628, 2196
BstNI CCWGG 1 cut(s) 2548
BstNSI RCATGY 4 cut(s) 74, 100, 1701, 2769
BstPI GGTNACC 1 cut(s) 2564
BstSCI CCNGG 2 cut(s) 124, 2546
BstSFI CTRYAG 2 cut(s) 411, 1252
BstSLI GKGCMC 1 cut(s) 675
BstUI CGCG 1 cut(s) 1373
BstV1I GCAGC 4 cut(s) 58, 135, 1160, 2706
BstV2I GAAGAC 2 cut(s) 1410, 1925
BstX2I RGATCY 4 cut(s) 1814, 2341, 2650, 2804
BstXI CCANNNNNNTGG 1 cut(s) 1591
BstYI RGATCY 4 cut(s) 1814, 2341, 2650, 2804
BstZ17I GTATAC 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 2159
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 5 cut(s) 395, 1048, 1286, 2489, 2739
BsuTUI ATCGAT 1 cut(s) 2159
BtgZI GCGATG 1 cut(s) 2059
BtrI CACGTC 2 cut(s) 1424, 1551
BtsI GCAGTG 2 cut(s) 11, 2664
BtsIMutI CAGTG 9 cut(s) 11, 1003, 1117, 1173, 1225, 1553, 2625, 2664, 2677
Cac8I GCNNGC 7 cut(s) 72, 1284, 1597, 2359, 2487, 2692, 2767
CaiI CAGNNNCTG 1 cut(s) 1622
CciI TCATGA 1 cut(s) 538
CfoI GCGC 2 cut(s) 834, 1799
Cfr13I GGNCC 4 cut(s) 530, 1285, 2675, 2737
ClaI ATCGAT 1 cut(s) 2159
CseI GACGC 1 cut(s) 1322
Csp6I GTAC 7 cut(s) 331, 576, 1233, 1666, 1845, 1975, 2456
CspCI CAANNNNNGTGG 8 cut(s) 669, 704, 771, 806, 1093, 1128, 2910, 2945
CviQI GTAC 7 cut(s) 331, 576, 1233, 1666, 1845, 1975, 2456
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 3 cut(s) 1479, 1506, 1749
EarI CTCTTC 3 cut(s) 1479, 1506, 1749
Ecl136II GAGCTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 687
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 2600
Eco47I GGWCC 2 cut(s) 530, 2675
Eco47III AGCGCT 1 cut(s) 1798
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 4 cut(s) 35, 1230, 1644, 1854
Eco91I GGTNACC 1 cut(s) 2564
EcoICRI GAGCTC 1 cut(s) 183
EcoO65I GGTNACC 1 cut(s) 2564
EcoRI GAATTC 1 cut(s) 1341
EcoRII CCWGG 1 cut(s) 2546
EcoT14I CCWWGG 1 cut(s) 687
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 687
FaqI GGGAC 1 cut(s) 1660
FauI CCCGC 3 cut(s) 242, 2354, 2942
FauNDI CATATG 2 cut(s) 439, 1137
FbaI TGATCA 1 cut(s) 1783
FblI GTMKAC 2 cut(s) 121, 497
Fnu4HI GCNGC 5 cut(s) 47, 149, 1149, 2695, 2937
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 5 cut(s) 47, 149, 1149, 2695, 2937
FspBI CTAG 8 cut(s) 1029, 1062, 1086, 1095, 1749, 1818, 2274, 2538
GlaI GCGC 2 cut(s) 833, 1798
GluI GCNGC 5 cut(s) 47, 149, 1149, 2695, 2937
GsaI CCCAGC 1 cut(s) 2088
GsuI CTGGAG 2 cut(s) 2410, 2929
HaeII RGCGCY 1 cut(s) 1800
HaeIII GGCC 5 cut(s) 395, 1048, 1286, 2489, 2739
HapII CCGG 3 cut(s) 125, 451, 2859
HgaI GACGC 1 cut(s) 1322
HhaI GCGC 2 cut(s) 834, 1799
Hin1I GRCGYC 1 cut(s) 733
Hin6I GCGC 2 cut(s) 832, 1797
HinP1I GCGC 2 cut(s) 832, 1797
HincII GTYRAC 2 cut(s) 1770, 2326
HindII GTYRAC 2 cut(s) 1770, 2326
HindIII AAGCTT 1 cut(s) 1707
HpaII CCGG 3 cut(s) 125, 451, 2859
HphI GGTGA 4 cut(s) 143, 846, 980, 2558
Hpy166II GTNNAC 7 cut(s) 122, 266, 498, 664, 1770, 2326, 2456
Hpy8I GTNNAC 7 cut(s) 122, 266, 498, 664, 1770, 2326, 2456
Hpy99I CGWCG 1 cut(s) 39
HpyAV CCTTC 7 cut(s) 499, 657, 846, 867, 1217, 1567, 2237
HpyCH4IV ACGT 5 cut(s) 666, 733, 1078, 1423, 1550
HpyF10VI GCNNNNNNNGC 5 cut(s) 362, 829, 1379, 1628, 2196
HpySE526I ACGT 5 cut(s) 666, 733, 1078, 1423, 1550
Hsp92I GRCGYC 1 cut(s) 733
HspAI GCGC 2 cut(s) 832, 1797
Kpn2I TCCGGA 1 cut(s) 450
Ksp22I TGATCA 1 cut(s) 1783
LmnI GCTCC 6 cut(s) 193, 246, 1227, 1940, 2391, 2846
Lsp1109I GCAGC 4 cut(s) 58, 135, 1160, 2706
LweI GCATC 8 cut(s) 105, 619, 1369, 1505, 1507, 1567, 1609, 2630
MaeI CTAG 8 cut(s) 1029, 1062, 1086, 1095, 1749, 1818, 2274, 2538
MaeII ACGT 5 cut(s) 666, 733, 1078, 1423, 1550
MaeIII GTNAC 7 cut(s) 131, 583, 852, 1398, 1551, 2024, 2564
MbiI CCGCTC 1 cut(s) 249
MfeI CAATTG 2 cut(s) 1181, 1934
MflI RGATCY 4 cut(s) 1814, 2341, 2650, 2804
MhlI GDGCHC 2 cut(s) 185, 675
MlyI GAGTC 6 cut(s) 279, 1289, 1682, 1769, 1915, 2594
MmeI TCCRAC 3 cut(s) 142, 1099, 1451
Mph1103I ATGCAT 1 cut(s) 76
MroI TCCGGA 1 cut(s) 450
MslI CAYNNNNRTG 3 cut(s) 87, 2718, 2960
MspA1I CMGCKG 1 cut(s) 2361
MspI CCGG 3 cut(s) 125, 451, 2859
MspR9I CCNGG 2 cut(s) 126, 2548
MunI CAATTG 2 cut(s) 1181, 1934
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 1 cut(s) 2548
MvnI CGCG 1 cut(s) 1373
MwoI GCNNNNNNNGC 5 cut(s) 362, 829, 1379, 1628, 2196
NciI CCSGG 1 cut(s) 126
NdeI CATATG 2 cut(s) 439, 1137
NlaIV GGNNCC 3 cut(s) 994, 2419, 2842
NmuCI GTSAC 4 cut(s) 131, 852, 1551, 2564
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 4 cut(s) 74, 100, 1701, 2769
PaeI GCATGC 2 cut(s) 74, 2769
PagI TCATGA 1 cut(s) 538
PciI ACATGT 1 cut(s) 96
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 6 cut(s) 557, 611, 1822, 1898, 2135, 2177
PkrI GCNGC 5 cut(s) 48, 150, 1150, 2696, 2938
Ple19I CGATCG 1 cut(s) 2163
PleI GAGTC 6 cut(s) 278, 1289, 1681, 1768, 1915, 2594
PpsI GAGTC 6 cut(s) 278, 1289, 1681, 1768, 1915, 2594
PscI ACATGT 1 cut(s) 96
PshBI ATTAAT 2 cut(s) 2397, 2880
Psp124BI GAGCTC 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 2546
PspEI GGTNACC 1 cut(s) 2564
PspFI CCCAGC 1 cut(s) 2084
PspGI CCWGG 1 cut(s) 2546
PspN4I GGNNCC 3 cut(s) 994, 2419, 2842
PspPI GGNCC 4 cut(s) 530, 1285, 2675, 2737
PstNI CAGNNNCTG 1 cut(s) 1622
PsuI RGATCY 4 cut(s) 1814, 2341, 2650, 2804
PvuI CGATCG 1 cut(s) 2163
RsaI GTAC 7 cut(s) 332, 577, 1234, 1667, 1846, 1976, 2457
RsaNI GTAC 7 cut(s) 331, 576, 1233, 1666, 1845, 1975, 2456
RseI CAYNNNNRTG 3 cut(s) 87, 2718, 2960
SacI GAGCTC 1 cut(s) 185
SatI GCNGC 5 cut(s) 47, 149, 1149, 2695, 2937
Sau96I GGNCC 4 cut(s) 530, 1285, 2675, 2737
SchI GAGTC 6 cut(s) 279, 1289, 1682, 1769, 1915, 2594
ScrFI CCNGG 2 cut(s) 126, 2548
SduI GDGCHC 2 cut(s) 185, 675
SfaNI GCATC 8 cut(s) 105, 619, 1369, 1505, 1507, 1567, 1609, 2630
SfcI CTRYAG 2 cut(s) 411, 1252
SinI GGWCC 2 cut(s) 530, 2675
SmiMI CAYNNNNRTG 3 cut(s) 87, 2718, 2960
SmlI CTYRAG 4 cut(s) 184, 807, 1790, 2334
SmoI CTYRAG 4 cut(s) 184, 807, 1790, 2334
SpeI ACTAGT 2 cut(s) 1061, 2273
SphI GCATGC 2 cut(s) 74, 2769
SsiI CCGC 8 cut(s) 237, 249, 546, 2361, 2501, 2769, 2937, 2949
SspI AATATT 1 cut(s) 1780
SspMI CTAG 8 cut(s) 1029, 1062, 1086, 1095, 1749, 1818, 2274, 2538
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 124, 2546
StyI CCWWGG 1 cut(s) 687
TaiI ACGT 5 cut(s) 669, 736, 1081, 1426, 1553
TaqI TCGA 6 cut(s) 34, 102, 1509, 2159, 2163, 2290
TatI WGTACW 4 cut(s) 575, 1232, 1844, 2455
TauI GCSGC 1 cut(s) 2939
TfiI GAWTC 6 cut(s) 557, 611, 1822, 1898, 2135, 2177
TscAI CASTG 8 cut(s) 1003, 1117, 1180, 1225, 1560, 2632, 2671, 2677
TseFI GTSAC 4 cut(s) 131, 852, 1551, 2564
TseI GCWGC 4 cut(s) 46, 148, 1148, 2694
Tsp45I GTSAC 4 cut(s) 131, 852, 1551, 2564
TspDTI ATGAA 9 cut(s) 426, 714, 1394, 1926, 2138, 2190, 2256, 2319, 2817
TspGWI ACGGA 2 cut(s) 52, 2734
TspRI CASTG 8 cut(s) 1003, 1117, 1180, 1225, 1560, 2632, 2671, 2677
VpaK11BI GGWCC 2 cut(s) 530, 2675
VspI ATTAAT 2 cut(s) 2397, 2880
XapI RAATTY 8 cut(s) 482, 977, 1341, 1354, 1440, 2032, 2591, 2834
XceI RCATGY 4 cut(s) 74, 100, 1701, 2769
XcmI CCANNNNNNNNNTGG 1 cut(s) 2482
XmiI GTMKAC 2 cut(s) 121, 497
XspI CTAG 8 cut(s) 1029, 1062, 1086, 1095, 1749, 1818, 2274, 2538
ZraI GACGTC 1 cut(s) 734
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.