AT4G11911

Protein STAY-GREEN 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009221
Physical Location & Seq
Forward (+)
7158086 .. 7159561
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G11911.1

Sequence Viewer

Length: 822 bp
ATGTGTAGCTTGATGGCAAGCTTGTTGCTCCCAGCAAACTTGAAACCAGATTTTTCAGACAAAGAGAGAAGTAGCTCAATCCCTTCAACTACTAGAAGTTCCAAGAGGAAGAAGCAATCTATGTTTCCTGTTGCAAGACTATTCGGACAGGCGATTTTCGAAGCTTCTAAACTGAATGTCAAGTTCTTAGGAGTTGATGAGAAGAAGCATCCACCGAATCTTCCAAGAACTTACACTTTCACTCATAGTGACATCACCGCTAAGTTAACTTTAGCTATTTCTCACTCGATCAATAACTCTCAGCTACAAGGATGGGCAAACAGGTTGTACCGAGACGAAGTAGTTGCCGAATGGAGGAAGGTGAAAAGTAACATGTCGCTTCACGTTCATTGTCACATTAGCGGTGATCATTTTCTTTTGGATCTCATTGCGGAACTTCGTTACTTCATATTTTGCAAGGAGTTACCCATGGTGTTAAAAGCTTTTGTTCATGGAGATGAGAACATGTTAAACAATTATCCTGAGCTACATGAAGCTTTTGTTTGGGTGTATTTCCATTCCAACATCCCCAAGTTCAACAAGGTCGAATGTTGGGGTCGGCTATGCGAGGCCACCTCGCACGATGGTTGTAAGACTCCCACGTGTGAAATCTTACCGGAACCGCCTTGCTTTGATAAGTGCAGCTGTTGTTTTCCGACGGTCAGCACGATCCCCTGGTCTCATTCTCATGGCTGCAGCCATGGTGAAGAGGATAATAATGTAGCTATAGCCGGAGGGAATCTATTGACCAAGTACATGTATAAGCGAAGTAAAAGAAAATAG

Protein Analysis

273

Amino Acids

31.14

Weight (kDa)

8.77

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 53 - 203 5.4e-55 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 258, 402, 431, 662
AclWI GGATC 2 cut(s) 429, 703
AcvI CACGTG 1 cut(s) 642
AfaI GTAC 2 cut(s) 329, 794
AfiI CCNNNNNNNGG 1 cut(s) 354
AflIII ACRYGT 4 cut(s) 372, 504, 641, 795
AgsI TTSAA 3 cut(s) 43, 87, 577
AjnI CCWGG 1 cut(s) 713
Alw26I GTCTC 2 cut(s) 327, 723
AlwI GGATC 2 cut(s) 429, 703
AoxI GGCC 1 cut(s) 609
ApeKI GCWGC 3 cut(s) 681, 732, 735
AsuHPI GGTGA 4 cut(s) 247, 373, 416, 755
AsuII TTCGAA 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 642
BbvI GCAGC 3 cut(s) 693, 719, 747
BccI CCATC 3 cut(s) 7, 306, 617
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 1 cut(s) 715
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 2 cut(s) 327, 723
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 733, 765
BisI GCNGC 3 cut(s) 682, 733, 736
BlsI GCNGC 3 cut(s) 683, 734, 737
Bme1390I CCNGG 1 cut(s) 715
BmiI GGNNCC 1 cut(s) 660
BmrFI CCNGG 1 cut(s) 715
BmsI GCATC 1 cut(s) 217
BplI GAGNNNNNCTC 2 cut(s) 599, 631
Bpu10I CCTNAGC 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 723
BsaJI CCNNGG 3 cut(s) 468, 713, 739
BsaWI WCCGGW 1 cut(s) 655
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 715
BseDI CCNNGG 3 cut(s) 468, 713, 739
BseGI GGATG 3 cut(s) 208, 317, 564
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 2 cut(s) 314, 513
BseXI GCAGC 3 cut(s) 693, 719, 747
BseYI CCCAGC 1 cut(s) 31
BsgI GTGCAG 1 cut(s) 700
BshFI GGCC 1 cut(s) 611
BsiSI CCGG 2 cut(s) 656, 771
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 2 cut(s) 327, 723
BsmBI CGTCTC 1 cut(s) 327
BsnI GGCC 1 cut(s) 611
Bso31I GGTCTC 1 cut(s) 723
Bsp119I TTCGAA 1 cut(s) 159
Bsp143I GATC 4 cut(s) 288, 406, 421, 708
Bsp19I CCATGG 2 cut(s) 468, 739
BspACI CCGC 4 cut(s) 258, 402, 431, 662
BspANI GGCC 1 cut(s) 611
BspCNI CTCAG 2 cut(s) 313, 514
BspLI GGNNCC 1 cut(s) 660
BspMAI CTGCAG 1 cut(s) 737
BspPI GGATC 2 cut(s) 429, 703
BspT104I TTCGAA 1 cut(s) 159
BspTNI GGTCTC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 426
BssECI CCNNGG 3 cut(s) 468, 713, 739
BssMI GATC 4 cut(s) 288, 406, 421, 708
BssT1I CCWWGG 2 cut(s) 468, 739
Bst2UI CCWGG 1 cut(s) 715
Bst4CI ACNGT 1 cut(s) 700
Bst6I CTCTTC 1 cut(s) 741
BstBAI YACGTR 1 cut(s) 642
BstBI TTCGAA 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 4 cut(s) 187, 261, 300, 522
BstDSI CCRYGG 2 cut(s) 468, 739
BstF5I GGATG 3 cut(s) 208, 317, 564
BstKTI GATC 4 cut(s) 291, 409, 424, 711
BstMAI GTCTC 2 cut(s) 327, 723
BstMBI GATC 4 cut(s) 288, 406, 421, 708
BstNI CCWGG 1 cut(s) 715
BstNSI RCATGY 3 cut(s) 376, 508, 799
BstSCI CCNGG 1 cut(s) 713
BstSFI CTRYAG 2 cut(s) 733, 765
BstV1I GCAGC 3 cut(s) 693, 719, 747
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuRI GGCC 1 cut(s) 611
BtgI CCRYGG 2 cut(s) 468, 739
BtsCI GGATG 3 cut(s) 208, 317, 564
Cac8I GCNNGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 328, 793
CviAII CATG 8 cut(s) 373, 469, 491, 505, 530, 728, 740, 796
CviQI GTAC 2 cut(s) 328, 793
DdeI CTNAG 4 cut(s) 187, 261, 300, 522
DpnI GATC 4 cut(s) 290, 408, 423, 710
DpnII GATC 4 cut(s) 288, 406, 421, 708
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
Eco130I CCWWGG 2 cut(s) 468, 739
Eco31I GGTCTC 1 cut(s) 723
Eco72I CACGTG 1 cut(s) 642
EcoRII CCWGG 1 cut(s) 713
EcoT14I CCWWGG 2 cut(s) 468, 739
ErhI CCWWGG 2 cut(s) 468, 739
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 8 cut(s) 376, 472, 494, 508, 533, 731, 743, 799
FatI CATG 8 cut(s) 372, 468, 490, 504, 529, 727, 739, 795
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 3 cut(s) 682, 733, 736
FokI GGATG 3 cut(s) 195, 324, 551
Fsp4HI GCNGC 3 cut(s) 682, 733, 736
FspBI CTAG 1 cut(s) 93
GluI GCNGC 3 cut(s) 682, 733, 736
GsaI CCCAGC 1 cut(s) 35
HaeIII GGCC 1 cut(s) 611
HapII CCGG 2 cut(s) 656, 771
Hin1II CATG 8 cut(s) 376, 472, 494, 508, 533, 731, 743, 799
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 4 cut(s) 19, 162, 480, 534
HinfI GANTC 3 cut(s) 217, 634, 778
HpaI GTTAAC 1 cut(s) 267
HpaII CCGG 2 cut(s) 656, 771
HphI GGTGA 4 cut(s) 247, 373, 416, 755
Hpy166II GTNNAC 1 cut(s) 267
Hpy188I TCNGA 3 cut(s) 58, 146, 696
Hpy188III TCNNGA 1 cut(s) 521
Hpy8I GTNNAC 1 cut(s) 267
Hpy99I CGWCG 1 cut(s) 700
HpyAV CCTTC 2 cut(s) 93, 352
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4IV ACGT 2 cut(s) 384, 641
HpyCH4V TGCA 4 cut(s) 134, 456, 681, 735
HpyF3I CTNAG 4 cut(s) 187, 261, 300, 522
HpySE526I ACGT 2 cut(s) 384, 641
Hsp92II CATG 8 cut(s) 376, 472, 494, 508, 533, 731, 743, 799
Ksp22I TGATCA 1 cut(s) 406
KspAI GTTAAC 1 cut(s) 267
Kzo9I GATC 4 cut(s) 288, 406, 421, 708
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 3 cut(s) 693, 719, 747
LweI GCATC 1 cut(s) 217
MaeI CTAG 1 cut(s) 93
MaeII ACGT 2 cut(s) 384, 641
MaeIII GTNAC 5 cut(s) 248, 368, 392, 440, 462
MalI GATC 4 cut(s) 290, 408, 423, 710
MboI GATC 4 cut(s) 288, 406, 421, 708
MboII GAAGA 4 cut(s) 121, 212, 214, 758
MflI RGATCY 1 cut(s) 421
MluCI AATT 1 cut(s) 514
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 2 cut(s) 585, 719
MnlI CCTC 6 cut(s) 99, 348, 601, 625, 742, 767
MseI TTAA 3 cut(s) 266, 476, 509
MslI CAYNNNNRTG 2 cut(s) 495, 726
MspA1I CMGCKG 1 cut(s) 684
MspI CCGG 2 cut(s) 656, 771
MspR9I CCNGG 1 cut(s) 715
MvaI CCWGG 1 cut(s) 715
NcoI CCATGG 2 cut(s) 468, 739
NdeII GATC 4 cut(s) 288, 406, 421, 708
NlaIII CATG 8 cut(s) 376, 472, 494, 508, 533, 731, 743, 799
NlaIV GGNNCC 1 cut(s) 660
NmuCI GTSAC 2 cut(s) 248, 392
NspI RCATGY 3 cut(s) 376, 508, 799
NspV TTCGAA 1 cut(s) 159
PciI ACATGT 3 cut(s) 372, 504, 795
PcsI WCGNNNNNNNCGW 1 cut(s) 704
PfeI GAWTC 2 cut(s) 217, 778
PkrI GCNGC 3 cut(s) 683, 734, 737
PleI GAGTC 1 cut(s) 628
PmaCI CACGTG 1 cut(s) 642
PmlI CACGTG 1 cut(s) 642
PpsI GAGTC 1 cut(s) 628
Ppu21I YACGTR 1 cut(s) 642
PscI ACATGT 3 cut(s) 372, 504, 795
Psp6I CCWGG 1 cut(s) 713
PspCI CACGTG 1 cut(s) 642
PspFI CCCAGC 1 cut(s) 31
PspGI CCWGG 1 cut(s) 713
PspN4I GGNNCC 1 cut(s) 660
PstI CTGCAG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 421
PvuII CAGCTG 1 cut(s) 684
RsaI GTAC 2 cut(s) 329, 794
RsaNI GTAC 2 cut(s) 328, 793
RseI CAYNNNNRTG 2 cut(s) 495, 726
SaqAI TTAA 3 cut(s) 266, 476, 509
SatI GCNGC 3 cut(s) 682, 733, 736
Sau3AI GATC 4 cut(s) 288, 406, 421, 708
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 1 cut(s) 715
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 2 cut(s) 733, 765
SfuI TTCGAA 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 495, 726
Sse9I AATT 1 cut(s) 514
SsiI CCGC 4 cut(s) 258, 402, 431, 662
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 713
StyI CCWWGG 2 cut(s) 468, 739
TaaI ACNGT 1 cut(s) 700
TaiI ACGT 2 cut(s) 387, 644
TaqI TCGA 3 cut(s) 159, 287, 585
TasI AATT 1 cut(s) 514
TatI WGTACW 1 cut(s) 792
TfiI GAWTC 2 cut(s) 217, 778
Tru1I TTAA 3 cut(s) 266, 476, 509
Tru9I TTAA 3 cut(s) 266, 476, 509
TseFI GTSAC 2 cut(s) 248, 392
TseI GCWGC 3 cut(s) 681, 732, 735
Tsp45I GTSAC 2 cut(s) 248, 392
TspDTI ATGAA 4 cut(s) 377, 436, 479, 546
XceI RCATGY 3 cut(s) 376, 508, 799
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.