pycom04g06370

Protein STAY-GREEN, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009221
Physical Location & Seq
Forward (+)
6450541 .. 6451985
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06370.1

Sequence Viewer

Length: 852 bp
ATGGGAGCTTTTACTGCTGCTTCTGTGCTTCCTTGGGATTTCAAGCCTTCACTTCCTCTCTCTCATCATAGAAGCTCTTTGTTTCACTATACAAGAACTCCCAAGAAAAGAAACAGGGCCATAGTTCCTGTTGCGAGGCTATTTGGGCCAGCAATATTTGAAGCATCGAAGCTGAAGGTTCTGTTTCTAGGAGTGGATGAAAAGAAGCACCCAGGGAAGCTTCCAAGAACTTACACACTCACTCATAGTGACATCACCTCAAAGCTCACTCTGGCAATTTCACAAACAATCGACAACTCTCAGTTGCAAGGGTGGTACAGCAAGTTGCAAAGAGATGAAGTTGTTGCTCAGTGGAAGAAAGTTAAGAACAAGATGTCTCTCCATGTTCACTGTCACATAAGTGGAGGTCATTTCCTCTTAGATTTGTTTGCAAGGCTCAGATACTTCATCTTCTGCAAAGAATTACCTGTGGTTTTGAAGGCCTTCGTCCATGGGGACGTCAACTTGTTCAACAGCTACCCAGAACTGCAGGACGCTTCGGTATGGATCTACTTCCACTCGAGCATTCCCGAATTCAACAAGGTGGAGTGCTGGGGCCCACTCGTCGACGCAGCAGCACCTTCTAGTGGGTCATCAGGCGGTGCCCACCACCAGGAAAACAACAGCGGCGGGGAGGGGGAGGAGGCAACTTCTCCAAGCAACTGGGACTTGCCAGAGACGTGTCAAGAGGAGTGCGACTGTTGCTTCACACCACTGACTTCGATTGCATGGTCCCAAGAGCTTTCCCATGCAAATCAAACTATGGTTGGGACCCACCAGAGCTTTCAGGGGCAAACCCAGGAAACAAACTGA

Protein Analysis

284

Amino Acids

31.7

Weight (kDa)

6.94

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 53 - 203 1.1e-56 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 501
AccB1I GGYRCC 1 cut(s) 641
AccI GTMKAC 1 cut(s) 606
AciI CCGC 3 cut(s) 639, 666, 669
AclWI GGATC 1 cut(s) 554
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 194
AcyI GRCGYC 1 cut(s) 498
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 2 cut(s) 626, 652
AflIII ACRYGT 1 cut(s) 719
AgsI TTSAA 5 cut(s) 43, 161, 478, 511, 577
AjiI CACGTC 1 cut(s) 720
AjnI CCWGG 3 cut(s) 211, 651, 837
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 8 cut(s) 8, 75, 172, 220, 265, 516, 781, 822
AluI AGCT 8 cut(s) 8, 75, 172, 220, 265, 516, 781, 822
Alw26I GTCTC 2 cut(s) 381, 710
AlwI GGATC 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 559
AoxI GGCC 4 cut(s) 117, 146, 480, 595
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 3 cut(s) 17, 611, 614
ApoI RAATTY 1 cut(s) 572
Asp700I GAANNNNTTC 1 cut(s) 482
AspS9I GGNCC 6 cut(s) 117, 146, 595, 596, 771, 810
AsuHPI GGTGA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 559
AvaII GGWCC 2 cut(s) 771, 810
BaeGI GKGCMC 2 cut(s) 599, 646
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 599
BbvI GCAGC 3 cut(s) 4, 623, 626
BciT130I CCWGG 3 cut(s) 213, 653, 839
BcoDI GTCTC 2 cut(s) 381, 710
BfaI CTAG 2 cut(s) 188, 624
BfmI CTRYAG 1 cut(s) 527
BisI GCNGC 4 cut(s) 18, 612, 615, 667
BlsI GCNGC 4 cut(s) 19, 613, 616, 668
Bme1390I CCNGG 3 cut(s) 213, 653, 839
Bme18I GGWCC 2 cut(s) 771, 810
BmeT110I CYCGRG 1 cut(s) 559
BmgBI CACGTC 1 cut(s) 720
BmgT120I GGNCC 6 cut(s) 117, 146, 595, 596, 771, 810
BmiI GGNNCC 6 cut(s) 596, 597, 643, 773, 811, 812
BmrFI CCNGG 3 cut(s) 213, 653, 839
BmrI ACTGGG 1 cut(s) 712
BmsI GCATC 1 cut(s) 173
BmuI ACTGGG 1 cut(s) 712
BsaHI GRCGYC 1 cut(s) 498
BsaJI CCNNGG 5 cut(s) 32, 211, 212, 490, 837
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 2 cut(s) 626, 652
Bse1I ACTGG 1 cut(s) 707
BseBI CCWGG 3 cut(s) 213, 653, 839
BseDI CCNNGG 5 cut(s) 32, 211, 212, 490, 837
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 2 cut(s) 626, 652
BseMII CTCAG 3 cut(s) 314, 362, 451
BseNI ACTGG 1 cut(s) 707
BseRI GAGGAG 2 cut(s) 695, 743
BseSI GKGCMC 2 cut(s) 599, 646
BseXI GCAGC 3 cut(s) 4, 623, 626
BseYI CCCAGC 1 cut(s) 591
BshFI GGCC 4 cut(s) 119, 148, 482, 597
BshNI GGYRCC 1 cut(s) 641
BsiHKCI CYCGRG 1 cut(s) 559
BslFI GGGAC 4 cut(s) 509, 719, 757, 823
BslI CCNNNNNNNGG 2 cut(s) 626, 652
BsmAI GTCTC 2 cut(s) 381, 710
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 4 cut(s) 509, 719, 757, 823
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 4 cut(s) 119, 148, 482, 597
BsoBI CYCGRG 1 cut(s) 559
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 2 cut(s) 599, 646
Bsp143I GATC 1 cut(s) 546
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 3 cut(s) 639, 666, 669
BspANI GGCC 4 cut(s) 119, 148, 482, 597
BspCNI CTCAG 3 cut(s) 313, 361, 450
BspLI GGNNCC 6 cut(s) 596, 597, 643, 773, 811, 812
BspMAI CTGCAG 1 cut(s) 531
BspPI GGATC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 641
BsrI ACTGG 1 cut(s) 707
BssECI CCNNGG 5 cut(s) 32, 211, 212, 490, 837
BssMI GATC 1 cut(s) 546
BssNI GRCGYC 1 cut(s) 498
BssT1I CCWWGG 2 cut(s) 32, 490
Bst2UI CCWGG 3 cut(s) 213, 653, 839
Bst4CI ACNGT 2 cut(s) 392, 740
BstACI GRCGYC 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 4 cut(s) 300, 348, 418, 437
BstDSI CCRYGG 1 cut(s) 490
BstF5I GGATG 1 cut(s) 202
BstKTI GATC 1 cut(s) 549
BstMAI GTCTC 2 cut(s) 381, 710
BstMBI GATC 1 cut(s) 546
BstMWI GCNNNNNNNGC 3 cut(s) 14, 145, 741
BstNI CCWGG 3 cut(s) 213, 653, 839
BstSCI CCNGG 3 cut(s) 211, 651, 837
BstSFI CTRYAG 1 cut(s) 527
BstSLI GKGCMC 2 cut(s) 599, 646
BstV1I GCAGC 3 cut(s) 4, 623, 626
BstX2I RGATCY 1 cut(s) 546
BstXI CCANNNNNNTGG 1 cut(s) 702
BstYI RGATCY 1 cut(s) 546
BsuRI GGCC 4 cut(s) 119, 148, 482, 597
BtgI CCRYGG 1 cut(s) 490
BtrI CACGTC 1 cut(s) 720
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 3 cut(s) 356, 388, 752
Cac8I GCNNGC 1 cut(s) 150
Cfr13I GGNCC 6 cut(s) 117, 146, 595, 596, 771, 810
CseI GACGC 2 cut(s) 542, 617
Csp6I GTAC 1 cut(s) 316
CviAII CATG 4 cut(s) 383, 491, 768, 788
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 4 cut(s) 300, 348, 418, 437
DpnI GATC 1 cut(s) 548
DpnII GATC 1 cut(s) 546
Eco130I CCWWGG 2 cut(s) 32, 490
Eco147I AGGCCT 1 cut(s) 482
Eco24I GRGCYC 1 cut(s) 599
Eco47I GGWCC 2 cut(s) 771, 810
Eco57I CTGAAG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 559
EcoO109I RGGNCCY 2 cut(s) 595, 810
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 3 cut(s) 211, 651, 837
EcoT14I CCWWGG 2 cut(s) 32, 490
EcoT38I GRGCYC 1 cut(s) 599
ErhI CCWWGG 2 cut(s) 32, 490
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 4 cut(s) 386, 494, 771, 791
FaqI GGGAC 4 cut(s) 509, 719, 757, 823
FatI CATG 4 cut(s) 382, 490, 767, 787
FauI CCCGC 1 cut(s) 662
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 18, 612, 615, 667
FokI GGATG 1 cut(s) 209
FriOI GRGCYC 1 cut(s) 599
Fsp4HI GCNGC 4 cut(s) 18, 612, 615, 667
FspBI CTAG 2 cut(s) 188, 624
GluI GCNGC 4 cut(s) 18, 612, 615, 667
GsaI CCCAGC 1 cut(s) 595
HaeIII GGCC 4 cut(s) 119, 148, 482, 597
HgaI GACGC 2 cut(s) 542, 617
Hin1I GRCGYC 1 cut(s) 498
Hin1II CATG 4 cut(s) 386, 494, 771, 791
HincII GTYRAC 2 cut(s) 502, 607
HindII GTYRAC 2 cut(s) 502, 607
HindIII AAGCTT 1 cut(s) 218
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 3 cut(s) 388, 502, 607
Hpy188I TCNGA 1 cut(s) 440
Hpy188III TCNNGA 2 cut(s) 569, 725
Hpy8I GTNNAC 3 cut(s) 388, 502, 607
Hpy99I CGWCG 2 cut(s) 608, 611
HpyAV CCTTC 5 cut(s) 57, 169, 472, 493, 630
HpyCH4III ACNGT 2 cut(s) 392, 740
HpyCH4IV ACGT 2 cut(s) 498, 719
HpyCH4V TGCA 7 cut(s) 307, 328, 431, 456, 529, 767, 791
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 145, 741
HpyF3I CTNAG 4 cut(s) 300, 348, 418, 437
HpySE526I ACGT 2 cut(s) 498, 719
Hsp92I GRCGYC 1 cut(s) 498
Hsp92II CATG 4 cut(s) 386, 494, 771, 791
KflI GGGWCCC 1 cut(s) 810
Kzo9I GATC 1 cut(s) 546
LmnI GCTCC 1 cut(s) 5
Lsp1109I GCAGC 3 cut(s) 4, 623, 626
LweI GCATC 1 cut(s) 173
MaeI CTAG 2 cut(s) 188, 624
MaeII ACGT 2 cut(s) 498, 719
MaeIII GTNAC 2 cut(s) 248, 392
MalI GATC 1 cut(s) 548
MboI GATC 1 cut(s) 546
MboII GAAGA 2 cut(s) 367, 442
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 2 cut(s) 599, 646
MluCI AATT 3 cut(s) 276, 461, 572
MnlI CCTC 9 cut(s) 66, 129, 268, 398, 425, 667, 673, 676, 721
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 666
MspR9I CCNGG 3 cut(s) 213, 653, 839
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 3 cut(s) 213, 653, 839
MwoI GCNNNNNNNGC 3 cut(s) 14, 145, 741
NcoI CCATGG 1 cut(s) 490
NdeII GATC 1 cut(s) 546
NlaIII CATG 4 cut(s) 386, 494, 771, 791
NlaIV GGNNCC 6 cut(s) 596, 597, 643, 773, 811, 812
NmuCI GTSAC 2 cut(s) 248, 392
OliI CACNNNNGTG 1 cut(s) 399
PaeR7I CTCGAG 1 cut(s) 559
PasI CCCWGGG 1 cut(s) 212
PceI AGGCCT 1 cut(s) 482
PctI GAATGC 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 482
PkrI GCNGC 4 cut(s) 19, 613, 616, 668
PpuMI RGGWCCY 1 cut(s) 810
Psp5II RGGWCCY 1 cut(s) 810
Psp6I CCWGG 3 cut(s) 211, 651, 837
PspFI CCCAGC 1 cut(s) 591
PspGI CCWGG 3 cut(s) 211, 651, 837
PspN4I GGNNCC 6 cut(s) 596, 597, 643, 773, 811, 812
PspOMI GGGCCC 1 cut(s) 595
PspPI GGNCC 6 cut(s) 117, 146, 595, 596, 771, 810
PspPPI RGGWCCY 1 cut(s) 810
PspXI VCTCGAGB 1 cut(s) 559
PstI CTGCAG 1 cut(s) 531
PsuI RGATCY 1 cut(s) 546
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 399
SalI GTCGAC 1 cut(s) 605
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 4 cut(s) 18, 612, 615, 667
Sau3AI GATC 1 cut(s) 546
Sau96I GGNCC 6 cut(s) 117, 146, 595, 596, 771, 810
ScrFI CCNGG 3 cut(s) 213, 653, 839
SduI GDGCHC 2 cut(s) 599, 646
SfaNI GCATC 1 cut(s) 173
SfcI CTRYAG 1 cut(s) 527
Sfr274I CTCGAG 1 cut(s) 559
SgrDI CGTCGACG 1 cut(s) 605
SinI GGWCC 2 cut(s) 771, 810
SlaI CTCGAG 1 cut(s) 559
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 1 cut(s) 559
SmoI CTYRAG 1 cut(s) 559
Sse9I AATT 3 cut(s) 276, 461, 572
SseBI AGGCCT 1 cut(s) 482
SsiI CCGC 3 cut(s) 639, 666, 669
SspI AATATT 1 cut(s) 156
SspMI CTAG 2 cut(s) 188, 624
StuI AGGCCT 1 cut(s) 482
StyD4I CCNGG 3 cut(s) 211, 651, 837
StyI CCWWGG 2 cut(s) 32, 490
TaaI ACNGT 2 cut(s) 392, 740
TaiI ACGT 2 cut(s) 501, 722
TaqI TCGA 5 cut(s) 167, 291, 560, 606, 761
TasI AATT 3 cut(s) 276, 461, 572
TauI GCSGC 1 cut(s) 669
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 3 cut(s) 356, 395, 759
TseFI GTSAC 2 cut(s) 248, 392
TseI GCWGC 3 cut(s) 17, 611, 614
Tsp45I GTSAC 2 cut(s) 248, 392
TspDTI ATGAA 3 cut(s) 213, 351, 436
TspRI CASTG 3 cut(s) 356, 395, 759
VpaK11BI GGWCC 2 cut(s) 771, 810
XapI RAATTY 1 cut(s) 572
XhoI CTCGAG 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 2 cut(s) 188, 624
ZraI GACGTC 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.