MD00G1131800.v1.1

Protein STAY-GREEN, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009221
Physical Location & Seq
Reverse (-)
28303995 .. 28305744
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1131800.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGGCGTTTTGGCTGCTGCTTCTGTGCTTCCTTCAAATTTCAAGCCTTCGCTCTCTCTCTCTCATCACAGAGGATCTTTGTTTCACTATACAAGAACACCCAAGAAAAGAAACAGGGCCATATTTCCTGTAAGGATTCAAACTTGGGTGCAGAGCGGGAGCGCCAACTTGACTACGCTCAAGTCTGCTTTTGGGTTTTCAGTTAATGCAAGGCTATTTGGGCCAGCAATATTTGAAGCATCAAAGCTGAAGGTTTTGTTTTTAGGAGTGGACGAAAAGAAGCACCTAGGAAAGCTTCCAAGAACTTACACACTCACTCATAGTGATGTCACCTCAAAACTCACTCTGGCAATTTCACAAACAATCGACGACGCTCAGTTGCAAGGGTGGTACAACAAGTTGCAAAGAGATGAAGTTGTGGCTCGGTGGAAGAAAGTCAAGAACAAGATGTCTCTCCATGTTCACTGTCACATAAGTGGATCTCATTTTCTCTTGGATTTGTTTGCAAGGCTCAGATACTTCATCTTCTGCAAAGAATTACCTGTGGTTTTGAAGGCCTTTGTTCATGGGGACTGCAACTTGTTCAACAGCTACCCAGAACTGCAGGAGGCTTTGGTATGGGTCCACTTCCACTCGAGCGTTCCAGAATTCAACAAGGTGGAGTGCTGGGGCACACTCATAGACGCCGCCGCCGCACCTTCTAGTGAATCATCAGGCGGGGCCCACCACCAGGAAATCAAGGGCAAGGGGGAGGAGGCAACTTCTCCAAGCAACTTGGACTTACCAGAGCCGTGCCAAGAGGACTGCGACTGTTGCTTCCCACCAGCGACTTCGATTGCATGGTGCAAATCAAACTAG

Protein Analysis

286

Amino Acids

31.78

Weight (kDa)

8.91

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 78 - 228 7.2e-55 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 156
AciI CCGC 5 cut(s) 156, 687, 690, 693, 717
AclWI GGATC 2 cut(s) 82, 487
AcsI RAATTY 2 cut(s) 37, 647
AcuI CTGAAG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 730
AgsI TTSAA 7 cut(s) 36, 43, 140, 236, 553, 586, 652
AjnI CCWGG 1 cut(s) 729
AleI CACNNNNGTG 1 cut(s) 474
AluBI AGCT 3 cut(s) 247, 295, 591
AluI AGCT 3 cut(s) 247, 295, 591
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 2 cut(s) 82, 487
Ama87I CYCGRG 1 cut(s) 634
AoxI GGCC 4 cut(s) 117, 221, 555, 720
ApaI GGGCCC 1 cut(s) 724
ApeKI GCWGC 2 cut(s) 14, 17
ApoI RAATTY 2 cut(s) 37, 647
AspA2I CCTAGG 1 cut(s) 286
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 5 cut(s) 117, 221, 622, 720, 721
AsuHPI GGTGA 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 1 cut(s) 622
AvrII CCTAGG 1 cut(s) 286
BaeGI GKGCMC 2 cut(s) 674, 724
BanII GRGCYC 1 cut(s) 724
BbvI GCAGC 1 cut(s) 4
BceAI ACGGC 1 cut(s) 775
BciT130I CCWGG 1 cut(s) 731
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 3 cut(s) 287, 702, 856
BfmI CTRYAG 1 cut(s) 602
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 5 cut(s) 15, 18, 687, 690, 693
BlnI CCTAGG 1 cut(s) 286
BlsI GCNGC 5 cut(s) 16, 19, 688, 691, 694
Bme1390I CCNGG 1 cut(s) 731
Bme18I GGWCC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 634
BmgT120I GGNCC 5 cut(s) 117, 221, 622, 720, 721
BmiI GGNNCC 3 cut(s) 623, 721, 722
BmrFI CCNGG 1 cut(s) 731
BmsI GCATC 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 684
BsaJI CCNNGG 1 cut(s) 286
Bsc4I CCNNNNNNNGG 1 cut(s) 730
BseBI CCWGG 1 cut(s) 731
BseDI CCNNGG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 730
BseMII CTCAG 2 cut(s) 389, 526
BseRI GAGGAG 1 cut(s) 767
BseSI GKGCMC 2 cut(s) 674, 724
BseXI GCAGC 1 cut(s) 4
BseYI CCCAGC 1 cut(s) 666
BsgI GTGCAG 1 cut(s) 170
BshFI GGCC 4 cut(s) 119, 223, 557, 722
BsiHKCI CYCGRG 1 cut(s) 634
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 1 cut(s) 730
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 4 cut(s) 119, 223, 557, 722
BsoBI CYCGRG 1 cut(s) 634
Bsp120I GGGCCC 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 674, 724
Bsp143I GATC 2 cut(s) 74, 479
BspACI CCGC 5 cut(s) 156, 687, 690, 693, 717
BspANI GGCC 4 cut(s) 119, 223, 557, 722
BspCNI CTCAG 2 cut(s) 388, 525
BspLI GGNNCC 3 cut(s) 623, 721, 722
BspMAI CTGCAG 1 cut(s) 606
BspPI GGATC 2 cut(s) 82, 487
BsrBI CCGCTC 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 2 cut(s) 74, 479
BssNI GRCGYC 1 cut(s) 684
BssT1I CCWWGG 1 cut(s) 286
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 2 cut(s) 467, 812
BstACI GRCGYC 1 cut(s) 684
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 2 cut(s) 375, 512
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 2 cut(s) 77, 482
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 2 cut(s) 74, 479
BstMWI GCNNNNNNNGC 3 cut(s) 220, 692, 813
BstNI CCWGG 1 cut(s) 731
BstSCI CCNGG 1 cut(s) 729
BstSFI CTRYAG 1 cut(s) 602
BstSLI GKGCMC 2 cut(s) 674, 724
BstV1I GCAGC 1 cut(s) 4
BstX2I RGATCY 2 cut(s) 74, 479
BstYI RGATCY 2 cut(s) 74, 479
BsuRI GGCC 4 cut(s) 119, 223, 557, 722
BtsIMutI CAGTG 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 225
CfoI GCGC 1 cut(s) 164
Cfr13I GGNCC 5 cut(s) 117, 221, 622, 720, 721
CseI GACGC 2 cut(s) 380, 692
Csp6I GTAC 1 cut(s) 391
CviAII CATG 3 cut(s) 458, 566, 840
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 2 cut(s) 375, 512
DpnI GATC 2 cut(s) 76, 481
DpnII GATC 2 cut(s) 74, 479
Eco130I CCWWGG 1 cut(s) 286
Eco147I AGGCCT 1 cut(s) 557
Eco24I GRGCYC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 720
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 729
EcoT14I CCWWGG 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 3 cut(s) 461, 569, 843
FaiI YATR 9 cut(s) 90, 122, 321, 459, 473, 567, 619, 680, 841
FaqI GGGAC 1 cut(s) 584
FatI CATG 3 cut(s) 457, 565, 839
FauI CCCGC 2 cut(s) 149, 710
Fnu4HI GCNGC 5 cut(s) 15, 18, 687, 690, 693
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 5 cut(s) 15, 18, 687, 690, 693
FspBI CTAG 3 cut(s) 287, 702, 856
GlaI GCGC 1 cut(s) 163
GluI GCNGC 5 cut(s) 15, 18, 687, 690, 693
GsaI CCCAGC 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 119, 223, 557, 722
HgaI GACGC 2 cut(s) 380, 692
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 3 cut(s) 461, 569, 843
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 2 cut(s) 136, 707
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 3 cut(s) 271, 463, 625
Hpy188I TCNGA 1 cut(s) 515
Hpy188III TCNNGA 2 cut(s) 439, 644
Hpy8I GTNNAC 3 cut(s) 271, 463, 625
Hpy99I CGWCG 2 cut(s) 371, 374
HpyAV CCTTC 5 cut(s) 42, 57, 244, 547, 708
HpyCH4III ACNGT 2 cut(s) 467, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 220, 692, 813
HpyF3I CTNAG 2 cut(s) 375, 512
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 3 cut(s) 461, 569, 843
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 2 cut(s) 74, 479
LmnI GCTCC 1 cut(s) 159
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 248
MaeI CTAG 3 cut(s) 287, 702, 856
MaeIII GTNAC 2 cut(s) 328, 467
MalI GATC 2 cut(s) 76, 481
MbiI CCGCTC 1 cut(s) 156
MboI GATC 2 cut(s) 74, 479
MboII GAAGA 2 cut(s) 442, 517
MflI RGATCY 2 cut(s) 74, 479
MhlI GDGCHC 2 cut(s) 674, 724
MluCI AATT 4 cut(s) 37, 351, 536, 647
MnlI CCTC 6 cut(s) 65, 343, 601, 745, 748, 793
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 2 cut(s) 324, 474
MspR9I CCNGG 1 cut(s) 731
MvaI CCWGG 1 cut(s) 731
MwoI GCNNNNNNNGC 3 cut(s) 220, 692, 813
NdeII GATC 2 cut(s) 74, 479
NlaIII CATG 3 cut(s) 461, 569, 843
NlaIV GGNNCC 3 cut(s) 623, 721, 722
NmuCI GTSAC 2 cut(s) 328, 467
OliI CACNNNNGTG 1 cut(s) 474
PaeR7I CTCGAG 1 cut(s) 634
PceI AGGCCT 1 cut(s) 557
PfeI GAWTC 2 cut(s) 136, 707
PkrI GCNGC 5 cut(s) 16, 19, 688, 691, 694
Psp6I CCWGG 1 cut(s) 729
PspFI CCCAGC 1 cut(s) 666
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 3 cut(s) 623, 721, 722
PspOMI GGGCCC 1 cut(s) 720
PspPI GGNCC 5 cut(s) 117, 221, 622, 720, 721
PspXI VCTCGAGB 1 cut(s) 634
PstI CTGCAG 1 cut(s) 606
PsuI RGATCY 2 cut(s) 74, 479
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 2 cut(s) 324, 474
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 5 cut(s) 15, 18, 687, 690, 693
Sau3AI GATC 2 cut(s) 74, 479
Sau96I GGNCC 5 cut(s) 117, 221, 622, 720, 721
ScrFI CCNGG 1 cut(s) 731
SduI GDGCHC 2 cut(s) 674, 724
SetI ASST 9 cut(s) 249, 255, 288, 297, 335, 544, 593, 660, 700
SfaNI GCATC 1 cut(s) 248
SfcI CTRYAG 1 cut(s) 602
Sfr274I CTCGAG 1 cut(s) 634
SinI GGWCC 1 cut(s) 622
SlaI CTCGAG 1 cut(s) 634
SmiMI CAYNNNNRTG 2 cut(s) 324, 474
SmlI CTYRAG 2 cut(s) 179, 634
SmoI CTYRAG 2 cut(s) 179, 634
Sse9I AATT 4 cut(s) 37, 351, 536, 647
SseBI AGGCCT 1 cut(s) 557
SsiI CCGC 5 cut(s) 156, 687, 690, 693, 717
SspI AATATT 1 cut(s) 231
SspMI CTAG 3 cut(s) 287, 702, 856
StuI AGGCCT 1 cut(s) 557
StyD4I CCNGG 1 cut(s) 729
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 2 cut(s) 467, 812
TaqI TCGA 3 cut(s) 366, 635, 833
TasI AATT 4 cut(s) 37, 351, 536, 647
TauI GCSGC 3 cut(s) 689, 692, 695
TfiI GAWTC 2 cut(s) 136, 707
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 1 cut(s) 470
TseFI GTSAC 2 cut(s) 328, 467
TseI GCWGC 2 cut(s) 14, 17
Tsp45I GTSAC 2 cut(s) 328, 467
TspDTI ATGAA 3 cut(s) 426, 511, 554
TspRI CASTG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 2 cut(s) 37, 647
XhoI CTCGAG 1 cut(s) 634
XmaJI CCTAGG 1 cut(s) 286
XspI CTAG 3 cut(s) 287, 702, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.