FvH4_5g01230

Protein STAY-GREEN, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009221
Physical Location & Seq
Reverse (-)
735260 .. 737590
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01230.t1

Sequence Viewer

Length: 825 bp
ATGGGTACTCTTACTGCTGCTGCTGCTGCTTCTTTGCTTCCTTCAGAGTTTAAACCTTCACTCTCCTTATCTGACTCCAAGAGCTCTCTGTTTTTCCAAAGAAGGTTACCCAAGAAGAGGAACCAAGCTTTAGTTCCTGTTGCAAGGCTATTTGGGCCAGCTATATTTGAATCATCAAAGCTTAAGGTTCTGTTTTTGGGAGTGGATGAAAAGAAGCACCCTGGGAATCTTCCAAGGACTTACACACTTACTCATAGTGATGTAACCTCAAAGCTCACTCTGGCCATCTCCCAGACTATAGACAACTCTCAGTTGCAAGGGTGGTACAACAAATTACAAAGAGATGAGGTTGTGGCAGAGTGGAAGAAAGTAAAGGACAAGATGTCTCTCCATGTTCACTGCCATATAAGTGGAGGTCATTTCCTTCTGGATTTGTTTTCAAGGCTGAGATACTTCATCTTCTGCAGAGAGCTTCCTGTTGTATTGAAGGCCTTCATTCATGGGGATGGCAACCTGTTCAACAGTTATCCAGAACTGGAGGAGGCTCTGGTCTGGGTTTACTTTCACTCCAACATTCCAGAATTCAACAAGGTTGAATGTTGGGGCCCACTGAGGGATGCATCATCACCATCTAATAGGACAAACCAGAAGGCAGCTTCCTCAAGCAAGCAGGAGGACTTTGTACCAGAGCCATGCCAAGAAGACTGTAGCTGTTGCTTCCCACCCCTGAGTTCCATCCCATGGCCCAAAGAGCTTAACCAGCCAAATGAAACTGGGTTTGGGGCCCAGCAGAGCTATCAGGGCCAAATCAGAGAACCAAACTAG

Protein Analysis

275

Amino Acids

30.93

Weight (kDa)

8.41

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 56 - 207 2.9e-56 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 741
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 3 cut(s) 7, 326, 684
AfiI CCNNNNNNNGG 3 cut(s) 117, 613, 741
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 6 cut(s) 170, 441, 487, 520, 586, 596
AhdI GACNNNNNGTC 1 cut(s) 382
AjnI CCWGG 1 cut(s) 220
AjuI GAANNNNNNNTTGG 4 cut(s) 117, 149, 762, 794
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 390
AoxI GGCC 7 cut(s) 155, 282, 489, 604, 743, 783, 802
ApaI GGGCCC 2 cut(s) 608, 787
ApeKI GCWGC 5 cut(s) 17, 20, 23, 26, 653
ApoI RAATTY 1 cut(s) 581
Asp700I GAANNNNTTC 1 cut(s) 491
AspS9I GGNCC 7 cut(s) 155, 604, 605, 744, 783, 784, 802
AsuHPI GGTGA 1 cut(s) 618
BaeGI GKGCMC 2 cut(s) 608, 787
BalI TGGCCA 1 cut(s) 284
BanII GRGCYC 3 cut(s) 86, 608, 787
BbsI GAAGAC 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 5 cut(s) 4, 7, 10, 13, 665
BccI CCATC 4 cut(s) 293, 500, 637, 743
BciT130I CCWGG 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 390
BfaI CTAG 1 cut(s) 823
BfmI CTRYAG 3 cut(s) 297, 463, 706
BfrI CTTAAG 1 cut(s) 182
BisI GCNGC 5 cut(s) 18, 21, 24, 27, 654
BlsI GCNGC 5 cut(s) 19, 22, 25, 28, 655
Bme1390I CCNGG 1 cut(s) 222
BmeRI GACNNNNNGTC 1 cut(s) 382
BmgT120I GGNCC 7 cut(s) 155, 604, 605, 744, 783, 784, 802
BmiI GGNNCC 5 cut(s) 122, 605, 606, 784, 785
BmrFI CCNGG 1 cut(s) 222
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 2 cut(s) 607, 629
BmuI ACTGGG 1 cut(s) 783
BpiI GAAGAC 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 646
BsaJI CCNNGG 4 cut(s) 220, 221, 233, 740
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 613, 741
Bse1I ACTGG 2 cut(s) 540, 778
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 4 cut(s) 220, 221, 233, 740
BseGI GGATG 4 cut(s) 211, 511, 622, 735
BseLI CCNNNNNNNGG 3 cut(s) 117, 613, 741
BseMII CTCAG 4 cut(s) 323, 437, 602, 719
BseNI ACTGG 2 cut(s) 540, 778
BseRI GAGGAG 1 cut(s) 554
BseSI GKGCMC 2 cut(s) 608, 787
BseXI GCAGC 5 cut(s) 4, 7, 10, 13, 665
BseYI CCCAGC 1 cut(s) 786
BshFI GGCC 7 cut(s) 157, 284, 491, 606, 745, 785, 804
BsiHKAI GWGCWC 1 cut(s) 86
BslI CCNNNNNNNGG 3 cut(s) 117, 613, 741
BsmAI GTCTC 1 cut(s) 390
BsnI GGCC 7 cut(s) 157, 284, 491, 606, 745, 785, 804
Bsp120I GGGCCC 2 cut(s) 604, 783
Bsp1286I GDGCHC 3 cut(s) 86, 608, 787
Bsp19I CCATGG 1 cut(s) 740
BspANI GGCC 7 cut(s) 157, 284, 491, 606, 745, 785, 804
BspCNI CTCAG 4 cut(s) 322, 438, 603, 720
BspLI GGNNCC 5 cut(s) 122, 605, 606, 784, 785
BspMAI CTGCAG 1 cut(s) 467
BspTI CTTAAG 1 cut(s) 182
BsrI ACTGG 2 cut(s) 540, 778
BssECI CCNNGG 4 cut(s) 220, 221, 233, 740
BssT1I CCWWGG 2 cut(s) 233, 740
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 2 cut(s) 524, 707
Bst6I CTCTTC 1 cut(s) 110
BstAFI CTTAAG 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 159, 668
BstDEI CTNAG 4 cut(s) 309, 446, 611, 728
BstDSI CCRYGG 1 cut(s) 740
BstEII GGTNACC 1 cut(s) 105
BstF5I GGATG 4 cut(s) 211, 511, 622, 735
BstMAI GTCTC 1 cut(s) 390
BstMWI GCNNNNNNNGC 6 cut(s) 23, 26, 154, 751, 760, 801
BstNI CCWGG 1 cut(s) 222
BstPI GGTNACC 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 3 cut(s) 297, 463, 706
BstSLI GKGCMC 2 cut(s) 608, 787
BstV1I GCAGC 5 cut(s) 4, 7, 10, 13, 665
BstV2I GAAGAC 1 cut(s) 708
BstXI CCANNNNNNTGG 1 cut(s) 410
BsuRI GGCC 7 cut(s) 157, 284, 491, 606, 745, 785, 804
BtgI CCRYGG 1 cut(s) 740
BtsCI GGATG 4 cut(s) 211, 511, 622, 735
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 2 cut(s) 397, 608
Cac8I GCNNGC 2 cut(s) 159, 668
Cfr13I GGNCC 7 cut(s) 155, 604, 605, 744, 783, 784, 802
Csp6I GTAC 3 cut(s) 6, 325, 683
CviAII CATG 4 cut(s) 392, 500, 693, 741
CviQI GTAC 3 cut(s) 6, 325, 683
DdeI CTNAG 4 cut(s) 309, 446, 611, 728
DraI TTTAAA 1 cut(s) 52
DriI GACNNNNNGTC 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 110
Eam1105I GACNNNNNGTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 110
Ecl136II GAGCTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 233, 740
Eco147I AGGCCT 1 cut(s) 491
Eco24I GRGCYC 3 cut(s) 86, 608, 787
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 84
EcoO109I RGGNCCY 2 cut(s) 604, 783
EcoO65I GGTNACC 1 cut(s) 105
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 220
EcoT14I CCWWGG 2 cut(s) 233, 740
EcoT22I ATGCAT 1 cut(s) 622
EcoT38I GRGCYC 3 cut(s) 86, 608, 787
ErhI CCWWGG 2 cut(s) 233, 740
FaeI CATG 4 cut(s) 395, 503, 696, 744
FaiI YATR 9 cut(s) 164, 255, 299, 393, 405, 407, 501, 694, 742
FatI CATG 4 cut(s) 391, 499, 692, 740
Fnu4HI GCNGC 5 cut(s) 18, 21, 24, 27, 654
FokI GGATG 4 cut(s) 218, 518, 629, 722
FriOI GRGCYC 3 cut(s) 86, 608, 787
Fsp4HI GCNGC 5 cut(s) 18, 21, 24, 27, 654
FspBI CTAG 1 cut(s) 823
GluI GCNGC 5 cut(s) 18, 21, 24, 27, 654
GsaI CCCAGC 1 cut(s) 790
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 7 cut(s) 157, 284, 491, 606, 745, 785, 804
Hin1II CATG 4 cut(s) 395, 503, 696, 744
HindIII AAGCTT 2 cut(s) 126, 179
HinfI GANTC 3 cut(s) 74, 170, 226
HphI GGTGA 1 cut(s) 618
Hpy166II GTNNAC 2 cut(s) 397, 559
Hpy188I TCNGA 3 cut(s) 46, 73, 812
Hpy188III TCNNGA 3 cut(s) 428, 530, 578
Hpy8I GTNNAC 2 cut(s) 397, 559
HpyAV CCTTC 7 cut(s) 51, 66, 96, 434, 481, 502, 643
HpyCH4III ACNGT 2 cut(s) 524, 707
HpyCH4V TGCA 4 cut(s) 143, 316, 465, 620
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 26, 154, 751, 760, 801
HpyF3I CTNAG 4 cut(s) 309, 446, 611, 728
Hsp92II CATG 4 cut(s) 395, 503, 696, 744
Lsp1109I GCAGC 5 cut(s) 4, 7, 10, 13, 665
LweI GCATC 2 cut(s) 607, 629
MaeI CTAG 1 cut(s) 823
MaeIII GTNAC 2 cut(s) 105, 262
MboII GAAGA 5 cut(s) 127, 221, 376, 451, 713
MhlI GDGCHC 3 cut(s) 86, 608, 787
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 2 cut(s) 332, 581
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 594
MnlI CCTC 9 cut(s) 111, 277, 340, 407, 532, 535, 606, 667, 670
Mox20I TGGCCA 1 cut(s) 284
Mph1103I ATGCAT 1 cut(s) 622
MroXI GAANNNNTTC 1 cut(s) 491
MscI TGGCCA 1 cut(s) 284
MseI TTAA 3 cut(s) 51, 183, 756
MslI CAYNNNNRTG 3 cut(s) 258, 408, 504
Msp20I TGGCCA 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 222
MssI GTTTAAAC 1 cut(s) 52
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 6 cut(s) 23, 26, 154, 751, 760, 801
NcoI CCATGG 1 cut(s) 740
NlaIII CATG 4 cut(s) 395, 503, 696, 744
NlaIV GGNNCC 5 cut(s) 122, 605, 606, 784, 785
NsiI ATGCAT 1 cut(s) 622
PasI CCCWGGG 1 cut(s) 221
PceI AGGCCT 1 cut(s) 491
PdmI GAANNNNTTC 1 cut(s) 491
PfeI GAWTC 2 cut(s) 170, 226
PflMI CCANNNNNTGG 1 cut(s) 741
PkrI GCNGC 5 cut(s) 19, 22, 25, 28, 655
PleI GAGTC 1 cut(s) 68
PmeI GTTTAAAC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 220
PspEI GGTNACC 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 786
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 5 cut(s) 122, 605, 606, 784, 785
PspOMI GGGCCC 2 cut(s) 604, 783
PspPI GGNCC 7 cut(s) 155, 604, 605, 744, 783, 784, 802
PstI CTGCAG 1 cut(s) 467
RsaI GTAC 3 cut(s) 7, 326, 684
RsaNI GTAC 3 cut(s) 6, 325, 683
RseI CAYNNNNRTG 3 cut(s) 258, 408, 504
SacI GAGCTC 1 cut(s) 86
SaqAI TTAA 3 cut(s) 51, 183, 756
SatI GCNGC 5 cut(s) 18, 21, 24, 27, 654
Sau96I GGNCC 7 cut(s) 155, 604, 605, 744, 783, 784, 802
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 3 cut(s) 86, 608, 787
SfaNI GCATC 2 cut(s) 607, 629
SfcI CTRYAG 3 cut(s) 297, 463, 706
SmiMI CAYNNNNRTG 3 cut(s) 258, 408, 504
SmlI CTYRAG 2 cut(s) 182, 661
SmoI CTYRAG 2 cut(s) 182, 661
Sse9I AATT 2 cut(s) 332, 581
SseBI AGGCCT 1 cut(s) 491
SspMI CTAG 1 cut(s) 823
SstI GAGCTC 1 cut(s) 86
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 2 cut(s) 233, 740
TaaI ACNGT 2 cut(s) 524, 707
TasI AATT 2 cut(s) 332, 581
TfiI GAWTC 2 cut(s) 170, 226
Tru1I TTAA 3 cut(s) 51, 183, 756
Tru9I TTAA 3 cut(s) 51, 183, 756
TscAI CASTG 2 cut(s) 404, 615
TseI GCWGC 5 cut(s) 17, 20, 23, 26, 653
TspDTI ATGAA 5 cut(s) 222, 445, 484, 488, 783
TspRI CASTG 2 cut(s) 404, 615
Van91I CCANNNNNTGG 1 cut(s) 741
Vha464I CTTAAG 1 cut(s) 182
XapI RAATTY 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 1 cut(s) 823
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.