Rroxscaffold_3G00256430

Protein STAY-GREEN, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009221
Physical Location & Seq
Reverse (-)
50771706 .. 50773779
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256430.1

Sequence Viewer

Length: 867 bp
ATGAGTACTCTTGCTGCTGCTTCTTTGCTTCCTTCAGAGTTTAAACCTTCACTCTCTTTATCTGACCCCAAAAGCTCTCTGTTTTACCATAGAAGAATACCGAAGAAGAGGAACCAAGCGTTAGTTCCTGTTGCAAGGATATTTGGGCCAGCCATATTTGAAGCATCAAAGCTAAAGGTTCTGTTCTTAGGAGTGGATGAAAAGAAGCACCCTGGGAATCTTCCAAGGACTTACACACTTACTCATAGTGATGTAACCTCAAAGCTCACTCTGGCCATCTCACAGACTATAAACAATTCTCAGTTGCAAGGGTGGTACAACAAATTACAAAGAGATGAGGTTGTGGCAGAATGGAGGAAAGTAAAGGACAAGATGTCTCTCCATGTTCACTGCCATATAAGTGGAGGTCATTTCCTTTTGGATTTGTTTTCAAGGCTGAGATACTTCATCTTCTGCAAAGAGCTTCCTGTTGTTTTGAAGGCCTTTATTCATGGAGATGGCAACCTGTTCAACAGTTACCCAGAATTGGAGGAGGCTTTGGTTTGGGTTTACTTTCACTCCAACATTCCAGAATTCAACAAGGTTGAATGTTGGGGTCCACTCAGGAATGCAACATCACCATCTGGTAGGGGACACCAGAAGGCAGCTTCCTCAAGCAACCAGGAGGACTTTGTGCCAGAACCATGCCAAGAGGACTGTAGCTGTTGCTTCCCACCATTGAGCTCCATCTCATGGCCCCAAGAGCTTCCCCAGCCAAATGAAACTGGATATGGGGCCCAGCAGAGCTTTCTGGGGGCAAACTCAAGAACCAAACTAAACTTTTTCTTTGGTTTTCTTTTATTGGTTAATGTAAGAACTAAGATGTAA

Protein Analysis

288

Amino Acids

32.57

Weight (kDa)

9.02

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 53 - 204 2.4e-56 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 732
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 2 cut(s) 7, 317
AfiI CCNNNNNNNGG 2 cut(s) 526, 732
AgsI TTSAA 6 cut(s) 161, 432, 478, 511, 577, 587
AhdI GACNNNNNGTC 1 cut(s) 373
AjnI CCWGG 2 cut(s) 211, 660
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AluBI AGCT 9 cut(s) 75, 172, 265, 463, 647, 702, 723, 745, 786
AluI AGCT 9 cut(s) 75, 172, 265, 463, 647, 702, 723, 745, 786
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 1 cut(s) 381
AoxI GGCC 5 cut(s) 146, 273, 480, 734, 774
ApaI GGGCCC 1 cut(s) 778
ApeKI GCWGC 3 cut(s) 14, 17, 644
ApoI RAATTY 1 cut(s) 572
AspS9I GGNCC 5 cut(s) 146, 596, 735, 774, 775
AsuHPI GGTGA 1 cut(s) 609
AvaII GGWCC 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 778
BalI TGGCCA 1 cut(s) 275
BanII GRGCYC 2 cut(s) 725, 778
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 2 cut(s) 4, 656
BccI CCATC 4 cut(s) 284, 491, 628, 734
BciT130I CCWGG 2 cut(s) 213, 662
BcoDI GTCTC 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 697
BisI GCNGC 3 cut(s) 15, 18, 645
BlsI GCNGC 3 cut(s) 16, 19, 646
BmcAI AGTACT 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 213, 662
Bme18I GGWCC 1 cut(s) 596
BmeRI GACNNNNNGTC 1 cut(s) 373
BmgT120I GGNCC 5 cut(s) 146, 596, 735, 774, 775
BmiI GGNNCC 5 cut(s) 113, 597, 737, 775, 776
BmrFI CCNGG 2 cut(s) 213, 662
BmsI GCATC 1 cut(s) 173
BpuEI CTTGAG 2 cut(s) 637, 787
BsaJI CCNNGG 3 cut(s) 211, 212, 224
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 732
Bse1I ACTGG 1 cut(s) 769
BseBI CCWGG 2 cut(s) 213, 662
BseDI CCNNGG 3 cut(s) 211, 212, 224
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 2 cut(s) 526, 732
BseMII CTCAG 3 cut(s) 314, 428, 616
BseNI ACTGG 1 cut(s) 769
BseRI GAGGAG 1 cut(s) 545
BseSI GKGCMC 1 cut(s) 778
BseXI GCAGC 2 cut(s) 4, 656
BseYI CCCAGC 2 cut(s) 750, 777
BshFI GGCC 5 cut(s) 148, 275, 482, 736, 776
BsiHKAI GWGCWC 1 cut(s) 725
BslFI GGGAC 1 cut(s) 645
BslI CCNNNNNNNGG 2 cut(s) 526, 732
BsmAI GTCTC 1 cut(s) 381
BsmFI GGGAC 1 cut(s) 645
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 5 cut(s) 148, 275, 482, 736, 776
Bsp120I GGGCCC 1 cut(s) 774
Bsp1286I GDGCHC 2 cut(s) 725, 778
BspANI GGCC 5 cut(s) 148, 275, 482, 736, 776
BspCNI CTCAG 3 cut(s) 313, 429, 615
BspLI GGNNCC 5 cut(s) 113, 597, 737, 775, 776
BsrI ACTGG 1 cut(s) 769
BssECI CCNNGG 3 cut(s) 211, 212, 224
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 2 cut(s) 213, 662
Bst4CI ACNGT 2 cut(s) 515, 698
Bst6I CTCTTC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 5 cut(s) 187, 300, 437, 602, 858
BstF5I GGATG 1 cut(s) 202
BstMAI GTCTC 1 cut(s) 381
BstMWI GCNNNNNNNGC 2 cut(s) 742, 751
BstNI CCWGG 2 cut(s) 213, 662
BstSCI CCNGG 2 cut(s) 211, 660
BstSFI CTRYAG 1 cut(s) 697
BstSLI GKGCMC 1 cut(s) 778
BstV1I GCAGC 2 cut(s) 4, 656
BstXI CCANNNNNNTGG 1 cut(s) 401
BsuRI GGCC 5 cut(s) 148, 275, 482, 736, 776
BtsCI GGATG 1 cut(s) 202
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 150
Cfr13I GGNCC 5 cut(s) 146, 596, 735, 774, 775
Csp6I GTAC 2 cut(s) 6, 316
CviAII CATG 4 cut(s) 383, 491, 684, 732
CviQI GTAC 2 cut(s) 6, 316
DdeI CTNAG 5 cut(s) 187, 300, 437, 602, 858
DraI TTTAAA 1 cut(s) 43
DriI GACNNNNNGTC 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 101
Eam1105I GACNNNNNGTC 1 cut(s) 373
EarI CTCTTC 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 723
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 482
Eco24I GRGCYC 2 cut(s) 725, 778
Eco47I GGWCC 1 cut(s) 596
Eco53kI GAGCTC 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 723
EcoO109I RGGNCCY 1 cut(s) 774
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 2 cut(s) 211, 660
EcoT14I CCWWGG 1 cut(s) 224
EcoT38I GRGCYC 2 cut(s) 725, 778
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 4 cut(s) 386, 494, 687, 735
FaqI GGGAC 1 cut(s) 645
FatI CATG 4 cut(s) 382, 490, 683, 731
Fnu4HI GCNGC 3 cut(s) 15, 18, 645
FokI GGATG 1 cut(s) 209
FriOI GRGCYC 2 cut(s) 725, 778
Fsp4HI GCNGC 3 cut(s) 15, 18, 645
GluI GCNGC 3 cut(s) 15, 18, 645
GsaI CCCAGC 2 cut(s) 754, 781
HaeIII GGCC 5 cut(s) 148, 275, 482, 736, 776
Hin1II CATG 4 cut(s) 386, 494, 687, 735
HinfI GANTC 1 cut(s) 217
HphI GGTGA 1 cut(s) 609
Hpy166II GTNNAC 3 cut(s) 388, 550, 599
Hpy188I TCNGA 2 cut(s) 37, 64
Hpy188III TCNNGA 3 cut(s) 569, 604, 804
Hpy8I GTNNAC 3 cut(s) 388, 550, 599
HpyAV CCTTC 4 cut(s) 42, 57, 472, 634
HpyCH4III ACNGT 2 cut(s) 515, 698
HpyCH4V TGCA 4 cut(s) 134, 307, 456, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 742, 751
HpyF3I CTNAG 5 cut(s) 187, 300, 437, 602, 858
Hsp92II CATG 4 cut(s) 386, 494, 687, 735
LmnI GCTCC 1 cut(s) 728
Lsp1109I GCAGC 2 cut(s) 4, 656
LweI GCATC 1 cut(s) 173
MaeIII GTNAC 2 cut(s) 253, 515
MboII GAAGA 5 cut(s) 105, 115, 118, 212, 442
MhlI GDGCHC 2 cut(s) 725, 778
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 4 cut(s) 295, 323, 524, 572
MluNI TGGCCA 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 585
Mox20I TGGCCA 1 cut(s) 275
MscI TGGCCA 1 cut(s) 275
MseI TTAA 2 cut(s) 42, 846
MslI CAYNNNNRTG 3 cut(s) 249, 399, 495
Msp20I TGGCCA 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 213, 662
MssI GTTTAAAC 1 cut(s) 43
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 2 cut(s) 213, 662
MwoI GCNNNNNNNGC 2 cut(s) 742, 751
NlaIII CATG 4 cut(s) 386, 494, 687, 735
NlaIV GGNNCC 5 cut(s) 113, 597, 737, 775, 776
PasI CCCWGGG 1 cut(s) 212
PceI AGGCCT 1 cut(s) 482
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 732
PkrI GCNGC 3 cut(s) 16, 19, 646
PmeI GTTTAAAC 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 725
Psp6I CCWGG 2 cut(s) 211, 660
PspFI CCCAGC 2 cut(s) 750, 777
PspGI CCWGG 2 cut(s) 211, 660
PspN4I GGNNCC 5 cut(s) 113, 597, 737, 775, 776
PspOMI GGGCCC 1 cut(s) 774
PspPI GGNCC 5 cut(s) 146, 596, 735, 774, 775
RsaI GTAC 2 cut(s) 7, 317
RsaNI GTAC 2 cut(s) 6, 316
RseI CAYNNNNRTG 3 cut(s) 249, 399, 495
SacI GAGCTC 1 cut(s) 725
SaqAI TTAA 2 cut(s) 42, 846
SatI GCNGC 3 cut(s) 15, 18, 645
Sau96I GGNCC 5 cut(s) 146, 596, 735, 774, 775
ScaI AGTACT 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 213, 662
SduI GDGCHC 2 cut(s) 725, 778
SfaNI GCATC 1 cut(s) 173
SfcI CTRYAG 1 cut(s) 697
SinI GGWCC 1 cut(s) 596
SmiMI CAYNNNNRTG 3 cut(s) 249, 399, 495
SmlI CTYRAG 2 cut(s) 652, 802
SmoI CTYRAG 2 cut(s) 652, 802
Sse9I AATT 4 cut(s) 295, 323, 524, 572
SseBI AGGCCT 1 cut(s) 482
SstI GAGCTC 1 cut(s) 725
StuI AGGCCT 1 cut(s) 482
StyD4I CCNGG 2 cut(s) 211, 660
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 2 cut(s) 515, 698
TasI AATT 4 cut(s) 295, 323, 524, 572
TatI WGTACW 1 cut(s) 5
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 2 cut(s) 42, 846
Tru9I TTAA 2 cut(s) 42, 846
TscAI CASTG 1 cut(s) 395
TseI GCWGC 3 cut(s) 14, 17, 644
TspDTI ATGAA 4 cut(s) 213, 436, 479, 774
TspRI CASTG 1 cut(s) 395
Van91I CCANNNNNTGG 1 cut(s) 732
VpaK11BI GGWCC 1 cut(s) 596
XapI RAATTY 1 cut(s) 572
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.