AT4G12830

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013164
Physical Location & Seq
Forward (+)
7531141 .. 7533587
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12830.1

Sequence Viewer

Length: 1182 bp
ATGGCGGTTCATATTCACAACCCTAACTTTTTCCACACTCATCATCTCCGAGCATCTCTTACTTCTGCCTCTGCATCACCTTCCTCATATCTCTTTCATAGACACTCTGCTTCAAAGTATCCTAGCTTCCTCTGCAAATCCAATAACAAAGATGACTATCTCATTGATGCTCCTGTTTCTGTTGGAGATGGATTCTCTTTTAGTGGAGGGAAGTATTCGGATCAACCGAGTCCATCAGATGAGTGGTTGAAGCAAGGGAAATGGGTTAAAGCTCATAGAGTTGGCGGCTCTGGTGTAGAAGCAAAAGATCCGATTTTCGGATTAACAATGGGAGCTAGCTCTCAAGCTTCAAAAGATCTTTTCCGATGGTTTAGTGTAGAATCCGGGAGTGTAGATAGTCCTCCTGTTATACTCATCCATGGATTCCCATCTCAGGCTTATTCTTACCGGAAAACTATTCCCGTTCTTTCAAAGAACTACCGCGCCATTGCTTTCGATTGGCTAGGATTTGGATTTTCTGACAAGCCTCAGGCTGGATATGGATTCAACTATACAATGGACGAGTTTGTTTCTTCGCTGGAGTCATTCATTGACGAAGTAACTACTAGTAAGGTGTCACTTGTGGTGCAGGGCTACTTCTCAGCTGCTGTTGTAAAATATGCAAGAAACCGACCAGACAAGATCAAGAATCTCATACTCTTGAATCCTCCTCTCACACCAGAACATGCTAAACTTCCATCAACATTGTCTGTATTCAGCAACTTTTTGCTTGGGGAAATTTTCTCTCAGGATCCTCTAAGAGCAAGTGACAAGCCTTTAACAAGCTGTGGTCCTTACAAAATGAAAGAAGACGATGCAATGGTTTATCGAAGACCTTATCTTACCTCAGGTTCTTCTGGTTTTGCACTTAATGCCATAAGCAGGTCCATGAAGAAAGAACTTAAGAAATATGCAGAAGAGATGAGGACATCACTAATGGATAAAAACTGGAAAATCCCGATCACCGTGTGCTGGGGACAACGAGATAGGTGGTTAAGCTATGAAGGAGTTGAAGAGTTCTGCAAGAGTTCGGGACACAACCTCGTCGAATTACCAAATGCTGGCCATCATGTACAAGAAGATTGTGGAGAGGAACTTGGAGGAATAATCTCAAGAATCATAAGCAAATCTGCTCTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

393

Amino Acids

43.76

Weight (kDa)

8.59

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 134 - 374 8.5e-20 alpha/beta hydrolase fold
Hydrolase_4 PF12146 136 - 371 3.9e-10 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 136 - 378 2.3e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12830
fragaria_vesca FvH4_2g17500 FvH4_2g17500
malus_domestica MD10G1148300.v1.1
prunus_persica Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1
pyrus_communis pycom05g14810 pycom10g12830
rosa_chinensis RchiOBHm_Chr6g0282231
rosa_laevigata RLG00000012899
rosa_multiflora Rmu_sc0014549.1_g000002
rosa_roxburghii Rroxscaffold_7G00186380
rosa_rugosa Rorug06G0146500
rosa_samantha Rh6BG262900 Rh6CG261800 Rh6DG253800
rosa_wichuraiana Rw6G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 912
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccII CGCG 1 cut(s) 483
AciI CCGC 3 cut(s) 5, 285, 481
AclWI GGATC 4 cut(s) 228, 302, 785, 798
AcoI YGGCCR 1 cut(s) 1102
AcsI RAATTY 1 cut(s) 777
AdeI CACNNNGTG 1 cut(s) 1008
AfaI GTAC 1 cut(s) 1113
AfiI CCNNNNNNNGG 6 cut(s) 317, 433, 533, 921, 1011, 1100
AflII CTTAAG 1 cut(s) 941
AgsI TTSAA 7 cut(s) 114, 250, 351, 471, 547, 703, 1052
AhlI ACTAGT 1 cut(s) 605
AluBI AGCT 8 cut(s) 126, 272, 335, 339, 347, 644, 825, 1038
AluI AGCT 8 cut(s) 126, 272, 335, 339, 347, 644, 825, 1038
AlwI GGATC 4 cut(s) 228, 302, 785, 798
AlwNI CAGNNNCTG 1 cut(s) 647
AoxI GGCC 1 cut(s) 1102
ApeKI GCWGC 1 cut(s) 644
ApoI RAATTY 1 cut(s) 777
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 2 cut(s) 830, 924
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 2 cut(s) 69, 994
AsuNHI GCTAGC 1 cut(s) 335
AvaII GGWCC 2 cut(s) 830, 924
AxyI CCTNAGG 2 cut(s) 528, 886
BalI TGGCCA 1 cut(s) 1104
BamHI GGATCC 1 cut(s) 790
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BbsI GAAGAC 2 cut(s) 855, 877
BbvI GCAGC 1 cut(s) 631
BccI CCATC 6 cut(s) 182, 241, 360, 436, 745, 1113
BciVI GTATCC 1 cut(s) 129
BcnI CCSGG 1 cut(s) 385
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 4 cut(s) 123, 336, 503, 606
BfrI CTTAAG 1 cut(s) 941
BfuAI ACCTGC 1 cut(s) 912
BfuI GTATCC 1 cut(s) 129
BglII AGATCT 1 cut(s) 355
BisI GCNGC 2 cut(s) 286, 645
BlsI GCNGC 2 cut(s) 287, 646
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 2 cut(s) 830, 924
BmgT120I GGNCC 2 cut(s) 830, 924
BmiI GGNNCC 1 cut(s) 792
BmrFI CCNGG 1 cut(s) 385
BmsI GCATC 4 cut(s) 62, 83, 157, 844
BmtI GCTAGC 1 cut(s) 339
BpiI GAAGAC 2 cut(s) 855, 877
BpmI CTGGAG 1 cut(s) 599
BpuEI CTTGAG 2 cut(s) 327, 1135
BpuMI CCSGG 1 cut(s) 385
BsaBI GATNNNNATC 2 cut(s) 156, 427
BsaJI CCNNGG 1 cut(s) 418
BsaWI WCCGGW 1 cut(s) 447
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 433, 533, 921, 1011, 1100
Bse1I ACTGG 1 cut(s) 992
Bse21I CCTNAGG 2 cut(s) 528, 886
Bse3DI GCAATG 2 cut(s) 486, 864
Bse8I GATNNNNATC 2 cut(s) 156, 427
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 1 cut(s) 414
BseJI GATNNNNATC 2 cut(s) 156, 427
BseLI CCNNNNNNNGG 6 cut(s) 317, 433, 533, 921, 1011, 1100
BseMI GCAATG 2 cut(s) 486, 864
BseMII CTCAG 5 cut(s) 446, 542, 654, 800, 900
BseNI ACTGG 1 cut(s) 992
BseRI GAGGAG 1 cut(s) 699
BseXI GCAGC 1 cut(s) 631
BseYI CCCAGC 1 cut(s) 1011
BsgI GTGCAG 1 cut(s) 647
Bsh1236I CGCG 1 cut(s) 483
BshFI GGCC 1 cut(s) 1104
BsiSI CCGG 2 cut(s) 384, 448
BslFI GGGAC 2 cut(s) 1029, 1086
BslI CCNNNNNNNGG 6 cut(s) 317, 433, 533, 921, 1011, 1100
BsmFI GGGAC 2 cut(s) 1029, 1086
BsnI GGCC 1 cut(s) 1104
Bsp1407I TGTACA 1 cut(s) 1111
Bsp143I GATC 6 cut(s) 220, 307, 355, 681, 790, 999
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 3 cut(s) 5, 285, 481
BspANI GGCC 1 cut(s) 1104
BspCNI CTCAG 5 cut(s) 445, 541, 653, 799, 899
BspFNI CGCG 1 cut(s) 483
BspLI GGNNCC 1 cut(s) 792
BspMI ACCTGC 1 cut(s) 912
BspOI GCTAGC 1 cut(s) 339
BspPI GGATC 4 cut(s) 228, 302, 785, 798
BspTI CTTAAG 1 cut(s) 941
BsrDI GCAATG 2 cut(s) 486, 864
BsrGI TGTACA 1 cut(s) 1111
BsrI ACTGG 1 cut(s) 992
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 6 cut(s) 220, 307, 355, 681, 790, 999
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 1 cut(s) 1006
Bst6I CTCTTC 2 cut(s) 951, 1047
BstAFI CTTAAG 1 cut(s) 941
BstAPI GCANNNNNTGC 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 1111
BstC8I GCNNGC 2 cut(s) 337, 1102
BstDEI CTNAG 6 cut(s) 432, 528, 640, 786, 797, 886
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 1 cut(s) 483
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 6 cut(s) 223, 310, 358, 684, 793, 1002
BstMBI GATC 6 cut(s) 220, 307, 355, 681, 790, 999
BstMWI GCNNNNNNNGC 2 cut(s) 132, 911
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 1 cut(s) 383
BstUI CGCG 1 cut(s) 483
BstV1I GCAGC 1 cut(s) 631
BstV2I GAAGAC 2 cut(s) 855, 877
BstX2I RGATCY 3 cut(s) 307, 355, 790
BstYI RGATCY 3 cut(s) 307, 355, 790
Bsu36I CCTNAGG 2 cut(s) 528, 886
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 1 cut(s) 1104
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 414
BveI ACCTGC 1 cut(s) 912
Cac8I GCNNGC 2 cut(s) 337, 1102
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 485
Cfr13I GGNCC 2 cut(s) 830, 924
Csp6I GTAC 1 cut(s) 1112
CviAII CATG 4 cut(s) 419, 725, 928, 1109
CviQI GTAC 1 cut(s) 1112
DdeI CTNAG 6 cut(s) 432, 528, 640, 786, 797, 886
DpnI GATC 6 cut(s) 222, 309, 357, 683, 792, 1001
DpnII GATC 6 cut(s) 220, 307, 355, 681, 790, 999
DraIII CACNNNGTG 1 cut(s) 1008
EaeI YGGCCR 1 cut(s) 1102
Eam1104I CTCTTC 2 cut(s) 951, 1047
EarI CTCTTC 2 cut(s) 951, 1047
Eco130I CCWWGG 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 830, 924
Eco81I CCTNAGG 2 cut(s) 528, 886
EcoT14I CCWWGG 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 4 cut(s) 422, 728, 931, 1112
FaqI GGGAC 2 cut(s) 1029, 1086
FatI CATG 4 cut(s) 418, 724, 927, 1108
Fnu4HI GCNGC 2 cut(s) 286, 645
FokI GGATG 1 cut(s) 401
Fsp4HI GCNGC 2 cut(s) 286, 645
FspBI CTAG 4 cut(s) 123, 336, 503, 606
GlaI GCGC 1 cut(s) 484
GluI GCNGC 2 cut(s) 286, 645
GsaI CCCAGC 1 cut(s) 1015
GsuI CTGGAG 1 cut(s) 599
HaeIII GGCC 1 cut(s) 1104
HapII CCGG 2 cut(s) 384, 448
HhaI GCGC 1 cut(s) 485
Hin1II CATG 4 cut(s) 422, 728, 931, 1112
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 9 cut(s) 192, 229, 380, 423, 543, 581, 688, 703, 1155
HpaII CCGG 2 cut(s) 384, 448
HphI GGTGA 2 cut(s) 69, 994
Hpy188I TCNGA 7 cut(s) 50, 220, 238, 312, 320, 365, 520
Hpy188III TCNNGA 6 cut(s) 685, 700, 788, 997, 1071, 1152
Hpy99I CGWCG 1 cut(s) 1088
HpyAV CCTTC 2 cut(s) 90, 1037
HpyCH4III ACNGT 1 cut(s) 1006
HpyCH4V TGCA 8 cut(s) 74, 135, 628, 662, 857, 905, 953, 1062
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 911
HpyF3I CTNAG 6 cut(s) 432, 528, 640, 786, 797, 886
Hsp92II CATG 4 cut(s) 422, 728, 931, 1112
HspAI GCGC 1 cut(s) 483
Kzo9I GATC 6 cut(s) 220, 307, 355, 681, 790, 999
LmnI GCTCC 2 cut(s) 175, 332
Lsp1109I GCAGC 1 cut(s) 631
LweI GCATC 4 cut(s) 62, 83, 157, 844
MaeI CTAG 4 cut(s) 123, 336, 503, 606
MaeIII GTNAC 3 cut(s) 598, 615, 806
MalI GATC 6 cut(s) 222, 309, 357, 683, 792, 1001
MboI GATC 6 cut(s) 220, 307, 355, 681, 790, 999
MboII GAAGA 8 cut(s) 564, 860, 882, 885, 943, 968, 1064, 1130
MflI RGATCY 3 cut(s) 307, 355, 790
MlsI TGGCCA 1 cut(s) 1104
MluCI AATT 2 cut(s) 777, 1088
MluNI TGGCCA 1 cut(s) 1104
MlyI GAGTC 2 cut(s) 238, 590
MmeI TCCRAC 1 cut(s) 163
Mox20I TGGCCA 1 cut(s) 1104
MscI TGGCCA 1 cut(s) 1104
MseI TTAA 6 cut(s) 267, 323, 818, 909, 942, 1034
Msp20I TGGCCA 1 cut(s) 1104
MspA1I CMGCKG 1 cut(s) 644
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 2 cut(s) 384, 448
MspR9I CCNGG 1 cut(s) 385
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 2 cut(s) 132, 911
NciI CCSGG 1 cut(s) 385
NcoI CCATGG 1 cut(s) 418
NdeII GATC 6 cut(s) 220, 307, 355, 681, 790, 999
NheI GCTAGC 1 cut(s) 335
NlaIII CATG 4 cut(s) 422, 728, 931, 1112
NlaIV GGNNCC 1 cut(s) 792
NmuCI GTSAC 2 cut(s) 615, 806
NspI RCATGY 1 cut(s) 728
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PfeI GAWTC 7 cut(s) 192, 380, 423, 543, 688, 703, 1155
PflMI CCANNNNNTGG 1 cut(s) 1100
PfoI TCCNGGA 1 cut(s) 383
PkrI GCNGC 2 cut(s) 287, 646
PleI GAGTC 2 cut(s) 237, 589
PpsI GAGTC 2 cut(s) 237, 589
PspFI CCCAGC 1 cut(s) 1011
PspN4I GGNNCC 1 cut(s) 792
PspPI GGNCC 2 cut(s) 830, 924
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 3 cut(s) 307, 355, 790
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 1 cut(s) 1113
RsaNI GTAC 1 cut(s) 1112
SaqAI TTAA 6 cut(s) 267, 323, 818, 909, 942, 1034
SatI GCNGC 2 cut(s) 286, 645
Sau3AI GATC 6 cut(s) 220, 307, 355, 681, 790, 999
Sau96I GGNCC 2 cut(s) 830, 924
SchI GAGTC 2 cut(s) 238, 590
ScrFI CCNGG 1 cut(s) 385
SfaNI GCATC 4 cut(s) 62, 83, 157, 844
SinI GGWCC 2 cut(s) 830, 924
SmlI CTYRAG 3 cut(s) 342, 941, 1150
SmoI CTYRAG 3 cut(s) 342, 941, 1150
SpeI ACTAGT 1 cut(s) 605
Sse9I AATT 2 cut(s) 777, 1088
SsiI CCGC 3 cut(s) 5, 285, 481
SspMI CTAG 4 cut(s) 123, 336, 503, 606
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 1006
TaqI TCGA 3 cut(s) 495, 868, 1086
TasI AATT 2 cut(s) 777, 1088
TatI WGTACW 1 cut(s) 1111
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 7 cut(s) 192, 380, 423, 543, 688, 703, 1155
Tru1I TTAA 6 cut(s) 267, 323, 818, 909, 942, 1034
Tru9I TTAA 6 cut(s) 267, 323, 818, 909, 942, 1034
TseFI GTSAC 2 cut(s) 615, 806
TseI GCWGC 1 cut(s) 644
Tsp45I GTSAC 2 cut(s) 615, 806
TspDTI ATGAA 5 cut(s) 86, 577, 857, 944, 1056
Van91I CCANNNNNTGG 1 cut(s) 1100
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 2 cut(s) 830, 924
XapI RAATTY 1 cut(s) 777
XceI RCATGY 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XspI CTAG 4 cut(s) 123, 336, 503, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.