MD10G1148300.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013164
Physical Location & Seq
Forward (+)
23586867 .. 23589941
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1148300.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGGCAGCAACGCAAGCCAAAACGCCTTCTCTATGTTATTATGCTCGTCAACTTCACAACCATATTCCTGCATCATCCCTATCACTTGTTGGTAATAATCTTAAATCCAACCACCACAAGAAGCCCCTCAAATTCACCTGCAGATCCAGCGGCCAGGATGATTATTACATTGATGCTCCTGTTGAAGTGGGTGATGGGTTTTCCTTTAGTGGAGGAAAGTATTCTGATGGACCAAGTCCTTCTGATGAATGGTTTAAGCAAGGGAAGATCGTGAAAGCTTATGCTGCTTTTGGTTCCGGCGAGAAGGCAAAAGACCCGATTTTTGGACTCGCAATGGGCGCTGATTCTCAGGCCTCCACTGATGGCTTACCAATTGAAACATGCTCTGTATATATGATTTTGTTCCATAGATGGTTTTGCGTTGAATCCGGAAGTGCTGATAATCCTTCAGTTATATTAATTCATGGTTTCCCTTCTCAGGCATACTCTTACCGCAAAGTTCTTCCCATACTTTCGAAGGACTATCACGCGATTGCATTTGATTGGCTCGGCTTTGGATTTTCAGATAAGCCTCAACCCAAATATGGATTTGACTACACTATGAATGAGTTTGTAGCATCCTTGGAGTCGTTCATAAACGAAGTTACTGTTAACAAGGTCACGCTCGTTGTCCAGGGATACTTTGCGCCGGTTGTAGTTAAATACGCTAGCGGTCATCAGGAAAAGATTGACAACTTAATACTCTTAAATCCTCCGCTAACAGAGAAGCATGCCAACCTTCCATCAACCTTGTCAATTTTCAGCAACTTTTTGTTGGGTGAAATATTTTCTCAGGATCCTCTGAGGGCTAGTGATAAAGCCTTGGAAAGCTCCGGACCTTACAAAATGAAAGAGGACGATGCTATGGTCTACAGAAATCCTTACCTCACATCCGGAGCCTCCGGTTTTGCACTAAATGCAATTAGCAGGGCCATGAAGAAAGACCTGAAGAAGTATGTGGGTGAAATGAAGACTATACTAACAGATGAAGACTGGAAAGTTCCGACCACAGTCTGTTGGGGCAAAAGAGACAGGTGGTTGAGTTATGACGGCGTTGAAGAATTCTGCAAGGATTCTAAGCATAAGCTTATTGAAATTCCTATGGCAGGCCATCACGTGCAGGAGGATCGCGGCGAAGAACTTGGGGAAGTCCTTAAGGGGATTATCAGAAGAAGCCGTTTCGGTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

410

Amino Acids

45.56

Weight (kDa)

6.64

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 149 - 389 9.2e-22 alpha/beta hydrolase fold
Hydrolase_4 PF12146 150 - 385 1.7e-10 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 151 - 394 2.6e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12830
fragaria_vesca FvH4_2g17500 FvH4_2g17500
malus_domestica MD10G1148300.v1.1
prunus_persica Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1
pyrus_communis pycom05g14810 pycom10g12830
rosa_chinensis RchiOBHm_Chr6g0282231
rosa_laevigata RLG00000012899
rosa_multiflora Rmu_sc0014549.1_g000002
rosa_roxburghii Rroxscaffold_7G00186380
rosa_rugosa Rorug06G0146500
rosa_samantha Rh6BG262900 Rh6CG261800 Rh6DG253800
rosa_wichuraiana Rw6G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 909
AccII CGCG 2 cut(s) 530, 1170
AccIII TCCGGA 3 cut(s) 428, 872, 932
AciI CCGC 5 cut(s) 150, 493, 711, 755, 1170
AclWI GGATC 4 cut(s) 138, 830, 843, 1173
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 3 cut(s) 131, 1100, 1134
AcuI CTGAAG 2 cut(s) 432, 1007
AcvI CACGTG 1 cut(s) 1156
AfiI CCNNNNNNNGG 4 cut(s) 323, 478, 584, 1145
AflII CTTAAG 1 cut(s) 1193
AgsI TTSAA 5 cut(s) 185, 377, 425, 1097, 1133
AjnI CCWGG 2 cut(s) 153, 672
AluBI AGCT 3 cut(s) 278, 870, 1126
AluI AGCT 3 cut(s) 278, 870, 1126
Alw26I GTCTC 1 cut(s) 1062
AlwI GGATC 4 cut(s) 138, 830, 843, 1173
Aor13HI TCCGGA 3 cut(s) 428, 872, 932
AoxI GGCC 4 cut(s) 151, 351, 969, 1147
ApeKI GCWGC 2 cut(s) 5, 284
ApoI RAATTY 3 cut(s) 131, 1100, 1134
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 220
AspLEI GCGC 2 cut(s) 341, 688
AspS9I GGNCC 3 cut(s) 230, 875, 969
AsuHPI GGTGA 4 cut(s) 127, 203, 830, 1013
AsuII TTCGAA 1 cut(s) 515
AsuNHI GCTAGC 1 cut(s) 707
AvaII GGWCC 2 cut(s) 230, 875
BamHI GGATCC 1 cut(s) 835
BbrPI CACGTG 1 cut(s) 1156
BbsI GAAGAC 2 cut(s) 1016, 1035
BbvI GCAGC 2 cut(s) 17, 271
BccI CCATC 6 cut(s) 188, 221, 356, 405, 790, 1158
BceAI ACGGC 2 cut(s) 1105, 1200
BcgI CGANNNNNNTGC 2 cut(s) 1148, 1182
BciT130I CCWGG 2 cut(s) 155, 674
BciVI GTATCC 1 cut(s) 671
BcoDI GTCTC 1 cut(s) 1062
BfaI CTAG 2 cut(s) 708, 849
BfmI CTRYAG 2 cut(s) 139, 910
BfoI RGCGCY 1 cut(s) 342
BfrI CTTAAG 1 cut(s) 1193
BfuAI ACCTGC 1 cut(s) 146
BfuI GTATCC 1 cut(s) 671
BisI GCNGC 4 cut(s) 6, 151, 285, 1171
BlsI GCNGC 4 cut(s) 7, 152, 286, 1172
Bme1390I CCNGG 2 cut(s) 155, 674
Bme18I GGWCC 2 cut(s) 230, 875
BmgT120I GGNCC 3 cut(s) 230, 875, 969
BmiI GGNNCC 3 cut(s) 295, 837, 937
BmrFI CCNGG 2 cut(s) 155, 674
BmsI GCATC 4 cut(s) 80, 163, 626, 889
BmtI GCTAGC 1 cut(s) 711
BoxI GACNNNNGTC 1 cut(s) 1049
BpiI GAAGAC 2 cut(s) 1016, 1035
Bpu14I TTCGAA 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 1156
BsaJI CCNNGG 3 cut(s) 621, 673, 861
BsaWI WCCGGW 4 cut(s) 428, 872, 932, 941
Bsc4I CCNNNNNNNGG 4 cut(s) 323, 478, 584, 1145
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 1037
Bse3DI GCAATG 1 cut(s) 339
BseAI TCCGGA 3 cut(s) 428, 872, 932
BseBI CCWGG 2 cut(s) 155, 674
BseDI CCNNGG 3 cut(s) 621, 673, 861
BseGI GGATG 4 cut(s) 74, 163, 617, 929
BseLI CCNNNNNNNGG 4 cut(s) 323, 478, 584, 1145
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 4 cut(s) 362, 491, 833, 845
BseNI ACTGG 1 cut(s) 1037
BseXI GCAGC 2 cut(s) 17, 271
BsgI GTGCAG 1 cut(s) 1178
Bsh1236I CGCG 2 cut(s) 530, 1170
BshFI GGCC 4 cut(s) 153, 353, 971, 1149
BsiSI CCGG 6 cut(s) 297, 429, 689, 873, 933, 942
BslI CCNNNNNNNGG 4 cut(s) 323, 478, 584, 1145
BsmAI GTCTC 1 cut(s) 1062
BsnI GGCC 4 cut(s) 153, 353, 971, 1149
Bsp119I TTCGAA 1 cut(s) 515
Bsp13I TCCGGA 3 cut(s) 428, 872, 932
Bsp143I GATC 4 cut(s) 143, 267, 835, 1165
BspACI CCGC 5 cut(s) 150, 493, 711, 755, 1170
BspANI GGCC 4 cut(s) 153, 353, 971, 1149
BspCNI CTCAG 4 cut(s) 361, 490, 834, 844
BspEI TCCGGA 3 cut(s) 428, 872, 932
BspFNI CGCG 2 cut(s) 530, 1170
BspLI GGNNCC 3 cut(s) 295, 837, 937
BspMAI CTGCAG 1 cut(s) 143
BspMI ACCTGC 1 cut(s) 146
BspOI GCTAGC 1 cut(s) 711
BspPI GGATC 4 cut(s) 138, 830, 843, 1173
BspT104I TTCGAA 1 cut(s) 515
BspTI CTTAAG 1 cut(s) 1193
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 1 cut(s) 1037
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 621, 673, 861
BssMI GATC 4 cut(s) 143, 267, 835, 1165
BssT1I CCWWGG 2 cut(s) 621, 861
Bst2UI CCWGG 2 cut(s) 155, 674
Bst4CI ACNGT 2 cut(s) 649, 1051
BstAFI CTTAAG 1 cut(s) 1193
BstAPI GCANNNNNTGC 1 cut(s) 956
BstBAI YACGTR 1 cut(s) 1156
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 4 cut(s) 15, 709, 771, 1147
BstDEI CTNAG 5 cut(s) 348, 477, 831, 842, 1116
BstENI CCTNNNNNAGG 1 cut(s) 1143
BstF5I GGATG 4 cut(s) 74, 163, 617, 929
BstFNI CGCG 2 cut(s) 530, 1170
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 2 cut(s) 341, 688
BstKTI GATC 4 cut(s) 146, 270, 838, 1168
BstMAI GTCTC 1 cut(s) 1062
BstMBI GATC 4 cut(s) 143, 267, 835, 1165
BstMWI GCNNNNNNNGC 5 cut(s) 14, 147, 284, 338, 956
BstNI CCWGG 2 cut(s) 155, 674
BstNSI RCATGY 2 cut(s) 384, 773
BstPAI GACNNNNGTC 1 cut(s) 1049
BstSCI CCNGG 2 cut(s) 153, 672
BstSFI CTRYAG 2 cut(s) 139, 910
BstUI CGCG 2 cut(s) 530, 1170
BstV1I GCAGC 2 cut(s) 17, 271
BstV2I GAAGAC 2 cut(s) 1016, 1035
BstX2I RGATCY 2 cut(s) 143, 835
BstYI RGATCY 2 cut(s) 143, 835
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 4 cut(s) 153, 353, 971, 1149
BtsCI GGATG 4 cut(s) 74, 163, 617, 929
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 4 cut(s) 15, 709, 771, 1147
CfoI GCGC 2 cut(s) 341, 688
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 3 cut(s) 230, 875, 969
CviAII CATG 4 cut(s) 381, 464, 770, 973
DdeI CTNAG 5 cut(s) 348, 477, 831, 842, 1116
DpnI GATC 4 cut(s) 145, 269, 837, 1167
DpnII GATC 4 cut(s) 143, 267, 835, 1165
EaeI YGGCCR 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 621, 861
Eco147I AGGCCT 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 230, 875
Eco57I CTGAAG 2 cut(s) 432, 1007
Eco72I CACGTG 1 cut(s) 1156
EcoNI CCTNNNNNAGG 1 cut(s) 1143
EcoRI GAATTC 1 cut(s) 1100
EcoRII CCWGG 2 cut(s) 153, 672
EcoT14I CCWWGG 2 cut(s) 621, 861
ErhI CCWWGG 2 cut(s) 621, 861
FaeI CATG 4 cut(s) 384, 467, 773, 976
FatI CATG 4 cut(s) 380, 463, 769, 972
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 4 cut(s) 6, 151, 285, 1171
FokI GGATG 4 cut(s) 61, 170, 604, 916
Fsp4HI GCNGC 4 cut(s) 6, 151, 285, 1171
FspBI CTAG 2 cut(s) 708, 849
GlaI GCGC 2 cut(s) 340, 687
GluI GCNGC 4 cut(s) 6, 151, 285, 1171
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 4 cut(s) 153, 353, 971, 1149
HapII CCGG 6 cut(s) 297, 429, 689, 873, 933, 942
HhaI GCGC 2 cut(s) 341, 688
Hin1II CATG 4 cut(s) 384, 467, 773, 976
Hin6I GCGC 2 cut(s) 339, 686
HinP1I GCGC 2 cut(s) 339, 686
HincII GTYRAC 2 cut(s) 50, 652
HindII GTYRAC 2 cut(s) 50, 652
HindIII AAGCTT 2 cut(s) 276, 1124
HinfI GANTC 5 cut(s) 327, 344, 425, 626, 1112
HpaI GTTAAC 1 cut(s) 652
HpaII CCGG 6 cut(s) 297, 429, 689, 873, 933, 942
HphI GGTGA 4 cut(s) 127, 203, 830, 1013
Hpy166II GTNNAC 3 cut(s) 50, 652, 910
Hpy188I TCNGA 6 cut(s) 226, 244, 565, 843, 1044, 1208
Hpy188III TCNNGA 6 cut(s) 271, 429, 719, 833, 873, 933
Hpy8I GTNNAC 3 cut(s) 50, 652, 910
HpyAV CCTTC 7 cut(s) 36, 249, 298, 456, 483, 511, 788
HpyCH4III ACNGT 2 cut(s) 649, 1051
HpyCH4IV ACGT 1 cut(s) 1155
HpyCH4V TGCA 7 cut(s) 71, 141, 536, 950, 959, 1107, 1159
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 147, 284, 338, 956
HpyF3I CTNAG 5 cut(s) 348, 477, 831, 842, 1116
HpySE526I ACGT 1 cut(s) 1155
Hsp92II CATG 4 cut(s) 384, 467, 773, 976
HspAI GCGC 2 cut(s) 339, 686
Kpn2I TCCGGA 3 cut(s) 428, 872, 932
KspAI GTTAAC 1 cut(s) 652
Kzo9I GATC 4 cut(s) 143, 267, 835, 1165
LmnI GCTCC 3 cut(s) 181, 875, 935
Lsp1109I GCAGC 2 cut(s) 17, 271
LweI GCATC 4 cut(s) 80, 163, 626, 889
MaeI CTAG 2 cut(s) 708, 849
MaeII ACGT 1 cut(s) 1155
MaeIII GTNAC 2 cut(s) 643, 658
MalI GATC 4 cut(s) 145, 269, 837, 1167
MboI GATC 4 cut(s) 143, 267, 835, 1165
MboII GAAGA 9 cut(s) 277, 494, 988, 1000, 1021, 1040, 1109, 1187, 1221
MfeI CAATTG 1 cut(s) 372
MflI RGATCY 2 cut(s) 143, 835
MluCI AATT 7 cut(s) 131, 372, 459, 795, 960, 1100, 1134
MlyI GAGTC 2 cut(s) 321, 635
MmeI TCCRAC 2 cut(s) 132, 1067
MroI TCCGGA 3 cut(s) 428, 872, 932
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 8 cut(s) 102, 255, 458, 651, 699, 737, 746, 1194
MspA1I CMGCKG 1 cut(s) 150
MspCI CTTAAG 1 cut(s) 1193
MspI CCGG 6 cut(s) 297, 429, 689, 873, 933, 942
MspR9I CCNGG 2 cut(s) 155, 674
MunI CAATTG 1 cut(s) 372
MvaI CCWGG 2 cut(s) 155, 674
MvnI CGCG 2 cut(s) 530, 1170
MwoI GCNNNNNNNGC 5 cut(s) 14, 147, 284, 338, 956
NdeII GATC 4 cut(s) 143, 267, 835, 1165
NheI GCTAGC 1 cut(s) 707
NlaIII CATG 4 cut(s) 384, 467, 773, 976
NlaIV GGNNCC 3 cut(s) 295, 837, 937
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 1 cut(s) 658
NspI RCATGY 2 cut(s) 384, 773
NspV TTCGAA 1 cut(s) 515
PaeI GCATGC 1 cut(s) 773
PaqCI CACCTGC 1 cut(s) 146
PceI AGGCCT 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 344, 425, 1112
PflFI GACNNNGTC 1 cut(s) 234
PkrI GCNGC 4 cut(s) 7, 152, 286, 1172
PleI GAGTC 2 cut(s) 321, 634
PmaCI CACGTG 1 cut(s) 1156
PmlI CACGTG 1 cut(s) 1156
PpsI GAGTC 2 cut(s) 321, 634
Ppu21I YACGTR 1 cut(s) 1156
PshAI GACNNNNGTC 1 cut(s) 1049
PshBI ATTAAT 1 cut(s) 458
Psp6I CCWGG 2 cut(s) 153, 672
PspCI CACGTG 1 cut(s) 1156
PspGI CCWGG 2 cut(s) 153, 672
PspN4I GGNNCC 3 cut(s) 295, 837, 937
PspPI GGNCC 3 cut(s) 230, 875, 969
PstI CTGCAG 1 cut(s) 143
PsuI RGATCY 2 cut(s) 143, 835
PsyI GACNNNGTC 1 cut(s) 234
SaqAI TTAA 8 cut(s) 102, 255, 458, 651, 699, 737, 746, 1194
SatI GCNGC 4 cut(s) 6, 151, 285, 1171
Sau3AI GATC 4 cut(s) 143, 267, 835, 1165
Sau96I GGNCC 3 cut(s) 230, 875, 969
SchI GAGTC 2 cut(s) 321, 635
ScrFI CCNGG 2 cut(s) 155, 674
SfaNI GCATC 4 cut(s) 80, 163, 626, 889
SfcI CTRYAG 2 cut(s) 139, 910
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 2 cut(s) 230, 875
SmlI CTYRAG 1 cut(s) 1193
SmoI CTYRAG 1 cut(s) 1193
SphI GCATGC 1 cut(s) 773
Sse9I AATT 7 cut(s) 131, 372, 459, 795, 960, 1100, 1134
SseBI AGGCCT 1 cut(s) 353
SsiI CCGC 5 cut(s) 150, 493, 711, 755, 1170
SspI AATATT 1 cut(s) 825
SspMI CTAG 2 cut(s) 708, 849
StuI AGGCCT 1 cut(s) 353
StyD4I CCNGG 2 cut(s) 153, 672
StyI CCWWGG 2 cut(s) 621, 861
TaaI ACNGT 2 cut(s) 649, 1051
TaiI ACGT 1 cut(s) 1158
TaqI TCGA 1 cut(s) 515
TaqII GACCGA 1 cut(s) 1211
TasI AATT 7 cut(s) 131, 372, 459, 795, 960, 1100, 1134
TauI GCSGC 2 cut(s) 153, 1173
TfiI GAWTC 3 cut(s) 344, 425, 1112
Tru1I TTAA 8 cut(s) 102, 255, 458, 651, 699, 737, 746, 1194
Tru9I TTAA 8 cut(s) 102, 255, 458, 651, 699, 737, 746, 1194
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 1 cut(s) 658
TseI GCWGC 2 cut(s) 5, 284
Tsp45I GTSAC 1 cut(s) 658
TspDTI ATGAA 8 cut(s) 261, 452, 617, 622, 902, 989, 1022, 1041
TspRI CASTG 1 cut(s) 364
Tth111I GACNNNGTC 1 cut(s) 234
Vha464I CTTAAG 1 cut(s) 1193
VpaK11BI GGWCC 2 cut(s) 230, 875
VspI ATTAAT 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 1143
XapI RAATTY 3 cut(s) 131, 1100, 1134
XceI RCATGY 2 cut(s) 384, 773
XmiI GTMKAC 1 cut(s) 909
XmnI GAANNNNTTC 1 cut(s) 220
XspI CTAG 2 cut(s) 708, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.