pycom10g12830

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013164
Physical Location & Seq
Forward (+)
16315450 .. 16318479
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12830.9

Sequence Viewer

Length: 1191 bp
ATGGCAGCAACGCAAGCCAAAACCCCTTCTCTTTGTTATTATGCTCGTCAACTTCACAACCATATTCCTGCATCATCCCTATCACTTGTTGGTAATAATCTTAAATCCAACCACCACAAGAAGCCCCTCAAATTCACCTGCAGATCCAGCGGCCAGGATGATTATTACATTGATGCTCCTGTTGAAATCGGTGATGGATTTTCCTTTAGTGGAGGAAAGTATTCTGATGGACCAAGTCCTTCTGATGAATGGTTTAAGCAAGGGAAGATAGTGAAAGCTTATGCTGCTTTTGGTTCCGGCGAGAAGGCAAAAGACCCGATTTTTGGACTCGCCATGGGTGCTGATTCTCAGGCCTCCACTGATGTTTTCAGATGGTTTTGCGTTGAATCCGGAAGTGCTGATAATCCTTCAGTTATATTAATTCATGGTTTTCCTTCTCAGGCATACTCTTATCGCAAAGTTCTTCCCATACTCTCAAAGGACTATCACGCGATTGCTTTTGATTGGCTCGGCTTTGGATTTTCAGATAAGCCTCAACCCAAATATGGATTTGACTACACTATGAACGAGTTTGTAGCATCCTTGGAGTCATTCATAAACGCAGTTACTGTTAACAAGGTCACGCTCGTTGTCCAGGGATACTTTGCGCCGGTTGTAGTTAAATACGCTAGCAGTCATCAGGAAAAGATTGACAACATAATACTCTTAAATCCTCCGCTAACAGAGAAGCATGCCAACCTTCCATCAACCTTGTCAATTTTCAGCAACTTTTTGTTGGGTGAAATATTTTCTCAGGATCCCTTGAGGGCTAGTGATAAAGCTTTGGAAAGCTCCGGACCTTACAAAATGAAAGAGGACGATGCTATGGTCTACAGAAATCCTTACCTCACATCTGGAGCATCCGGTTTTGCACTAAATGCAATTAGCAGGGCCATGAAGAAAGAGCTGAAGAAGTATGTGGGTGAAATGAAGACAATACTAACAGATGAAGACTGGAAAGTTCCGACCACAGTCTGTTGGGGCAAAAGAGACAGGTGGTTGAGTTATGATGGCGTTGAAGAATTCTGCAAGGATTCTAAGCATAAGCTTATTGAAATTCCTATGGCAGGCCATCACGTGCAGGAGGATCGCGGCGAAGAACTTGGGGAAGTGCTTAAGGGGATTATCAGAAGAAGCCGTTTTGGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

397

Amino Acids

44.1

Weight (kDa)

7.16

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12830
fragaria_vesca FvH4_2g17500 FvH4_2g17500
malus_domestica MD10G1148300.v1.1
prunus_persica Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1
pyrus_communis pycom05g14810 pycom10g12830
rosa_chinensis RchiOBHm_Chr6g0282231
rosa_laevigata RLG00000012899
rosa_multiflora Rmu_sc0014549.1_g000002
rosa_roxburghii Rroxscaffold_7G00186380
rosa_rugosa Rorug06G0146500
rosa_samantha Rh6BG262900 Rh6CG261800 Rh6DG253800
rosa_wichuraiana Rw6G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 870
AccII CGCG 2 cut(s) 491, 1131
AccIII TCCGGA 2 cut(s) 389, 833
AciI CCGC 3 cut(s) 150, 716, 1131
AclWI GGATC 4 cut(s) 138, 791, 804, 1134
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 3 cut(s) 131, 1061, 1095
AcuI CTGAAG 2 cut(s) 393, 968
AcvI CACGTG 1 cut(s) 1117
AfiI CCNNNNNNNGG 3 cut(s) 323, 545, 1106
AflII CTTAAG 1 cut(s) 1154
AgsI TTSAA 4 cut(s) 185, 386, 1058, 1094
AjnI CCWGG 2 cut(s) 153, 633
AluBI AGCT 5 cut(s) 278, 821, 831, 946, 1087
AluI AGCT 5 cut(s) 278, 821, 831, 946, 1087
Alw26I GTCTC 1 cut(s) 1023
AlwI GGATC 4 cut(s) 138, 791, 804, 1134
AlwNI CAGNNNCTG 1 cut(s) 608
Aor13HI TCCGGA 2 cut(s) 389, 833
AoxI GGCC 4 cut(s) 151, 351, 930, 1108
ApeKI GCWGC 2 cut(s) 5, 284
ApoI RAATTY 3 cut(s) 131, 1061, 1095
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AseI ATTAAT 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 220
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 3 cut(s) 230, 836, 930
AsuHPI GGTGA 4 cut(s) 127, 203, 791, 974
AsuNHI GCTAGC 1 cut(s) 668
AvaII GGWCC 2 cut(s) 230, 836
BamHI GGATCC 1 cut(s) 796
BbrPI CACGTG 1 cut(s) 1117
BbsI GAAGAC 2 cut(s) 977, 996
BbvI GCAGC 2 cut(s) 17, 271
BccI CCATC 6 cut(s) 188, 221, 366, 751, 1043, 1119
BceAI ACGGC 1 cut(s) 1161
BcgI CGANNNNNNTGC 2 cut(s) 1109, 1143
BciT130I CCWGG 2 cut(s) 155, 635
BciVI GTATCC 1 cut(s) 632
BcoDI GTCTC 1 cut(s) 1023
BfaI CTAG 2 cut(s) 669, 810
BfmI CTRYAG 2 cut(s) 139, 871
BfrI CTTAAG 1 cut(s) 1154
BfuAI ACCTGC 1 cut(s) 146
BfuI GTATCC 1 cut(s) 632
BisI GCNGC 4 cut(s) 6, 151, 285, 1132
BlsI GCNGC 4 cut(s) 7, 152, 286, 1133
Bme1390I CCNGG 2 cut(s) 155, 635
Bme18I GGWCC 2 cut(s) 230, 836
BmgT120I GGNCC 3 cut(s) 230, 836, 930
BmiI GGNNCC 2 cut(s) 295, 798
BmrFI CCNGG 2 cut(s) 155, 635
BmsI GCATC 5 cut(s) 80, 163, 587, 850, 908
BmtI GCTAGC 1 cut(s) 672
BoxI GACNNNNGTC 1 cut(s) 1010
BpiI GAAGAC 2 cut(s) 977, 996
BpmI CTGGAG 1 cut(s) 915
BpuEI CTTGAG 1 cut(s) 823
BsaAI YACGTR 1 cut(s) 1117
BsaJI CCNNGG 3 cut(s) 333, 582, 634
BsaWI WCCGGW 3 cut(s) 389, 833, 902
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 545, 1106
Bse118I RCCGGY 1 cut(s) 649
Bse1I ACTGG 1 cut(s) 998
BseAI TCCGGA 2 cut(s) 389, 833
BseBI CCWGG 2 cut(s) 155, 635
BseDI CCNNGG 3 cut(s) 333, 582, 634
BseGI GGATG 4 cut(s) 74, 163, 578, 899
BseLI CCNNNNNNNGG 3 cut(s) 323, 545, 1106
BseMII CTCAG 3 cut(s) 362, 452, 806
BseNI ACTGG 1 cut(s) 998
BseXI GCAGC 2 cut(s) 17, 271
BsgI GTGCAG 1 cut(s) 1139
Bsh1236I CGCG 2 cut(s) 491, 1131
BshFI GGCC 4 cut(s) 153, 353, 932, 1110
BsiSI CCGG 5 cut(s) 297, 390, 650, 834, 903
BslI CCNNNNNNNGG 3 cut(s) 323, 545, 1106
BsmAI GTCTC 1 cut(s) 1023
BsnI GGCC 4 cut(s) 153, 353, 932, 1110
Bsp13I TCCGGA 2 cut(s) 389, 833
Bsp143I GATC 3 cut(s) 143, 796, 1126
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 3 cut(s) 150, 716, 1131
BspANI GGCC 4 cut(s) 153, 353, 932, 1110
BspCNI CTCAG 3 cut(s) 361, 451, 805
BspEI TCCGGA 2 cut(s) 389, 833
BspFNI CGCG 2 cut(s) 491, 1131
BspLI GGNNCC 2 cut(s) 295, 798
BspMAI CTGCAG 1 cut(s) 143
BspMI ACCTGC 1 cut(s) 146
BspOI GCTAGC 1 cut(s) 672
BspPI GGATC 4 cut(s) 138, 791, 804, 1134
BspTI CTTAAG 1 cut(s) 1154
BsrFI RCCGGY 1 cut(s) 649
BsrI ACTGG 1 cut(s) 998
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 3 cut(s) 333, 582, 634
BssMI GATC 3 cut(s) 143, 796, 1126
BssT1I CCWWGG 2 cut(s) 333, 582
Bst2UI CCWGG 2 cut(s) 155, 635
Bst4CI ACNGT 2 cut(s) 610, 1012
BstAFI CTTAAG 1 cut(s) 1154
BstAPI GCANNNNNTGC 1 cut(s) 917
BstBAI YACGTR 1 cut(s) 1117
BstC8I GCNNGC 4 cut(s) 15, 670, 732, 1108
BstDEI CTNAG 4 cut(s) 348, 438, 792, 1077
BstDSI CCRYGG 1 cut(s) 333
BstENI CCTNNNNNAGG 1 cut(s) 1104
BstF5I GGATG 4 cut(s) 74, 163, 578, 899
BstFNI CGCG 2 cut(s) 491, 1131
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 3 cut(s) 146, 799, 1129
BstMAI GTCTC 1 cut(s) 1023
BstMBI GATC 3 cut(s) 143, 796, 1126
BstMWI GCNNNNNNNGC 5 cut(s) 14, 147, 284, 338, 917
BstNI CCWGG 2 cut(s) 155, 635
BstNSI RCATGY 1 cut(s) 734
BstPAI GACNNNNGTC 1 cut(s) 1010
BstSCI CCNGG 2 cut(s) 153, 633
BstSFI CTRYAG 2 cut(s) 139, 871
BstUI CGCG 2 cut(s) 491, 1131
BstV1I GCAGC 2 cut(s) 17, 271
BstV2I GAAGAC 2 cut(s) 977, 996
BstX2I RGATCY 2 cut(s) 143, 796
BstYI RGATCY 2 cut(s) 143, 796
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 4 cut(s) 153, 353, 932, 1110
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 74, 163, 578, 899
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 4 cut(s) 15, 670, 732, 1108
CaiI CAGNNNCTG 1 cut(s) 608
CfoI GCGC 1 cut(s) 649
Cfr10I RCCGGY 1 cut(s) 649
Cfr13I GGNCC 3 cut(s) 230, 836, 930
CviAII CATG 4 cut(s) 334, 425, 731, 934
DdeI CTNAG 4 cut(s) 348, 438, 792, 1077
DpnI GATC 3 cut(s) 145, 798, 1128
DpnII GATC 3 cut(s) 143, 796, 1126
EaeI YGGCCR 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 333, 582
Eco147I AGGCCT 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 230, 836
Eco57I CTGAAG 2 cut(s) 393, 968
Eco72I CACGTG 1 cut(s) 1117
EcoNI CCTNNNNNAGG 1 cut(s) 1104
EcoRI GAATTC 1 cut(s) 1061
EcoRII CCWGG 2 cut(s) 153, 633
EcoT14I CCWWGG 2 cut(s) 333, 582
ErhI CCWWGG 2 cut(s) 333, 582
FaeI CATG 4 cut(s) 337, 428, 734, 937
FatI CATG 4 cut(s) 333, 424, 730, 933
FblI GTMKAC 1 cut(s) 870
Fnu4HI GCNGC 4 cut(s) 6, 151, 285, 1132
FokI GGATG 4 cut(s) 61, 170, 565, 886
Fsp4HI GCNGC 4 cut(s) 6, 151, 285, 1132
FspBI CTAG 2 cut(s) 669, 810
GlaI GCGC 1 cut(s) 648
GluI GCNGC 4 cut(s) 6, 151, 285, 1132
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 4 cut(s) 153, 353, 932, 1110
HapII CCGG 5 cut(s) 297, 390, 650, 834, 903
HhaI GCGC 1 cut(s) 649
Hin1II CATG 4 cut(s) 337, 428, 734, 937
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 2 cut(s) 50, 613
HindII GTYRAC 2 cut(s) 50, 613
HindIII AAGCTT 3 cut(s) 276, 819, 1085
HinfI GANTC 5 cut(s) 327, 344, 386, 587, 1073
HpaI GTTAAC 1 cut(s) 613
HpaII CCGG 5 cut(s) 297, 390, 650, 834, 903
HphI GGTGA 4 cut(s) 127, 203, 791, 974
Hpy166II GTNNAC 3 cut(s) 50, 613, 871
Hpy188I TCNGA 6 cut(s) 226, 244, 371, 526, 1005, 1169
Hpy188III TCNNGA 5 cut(s) 390, 680, 794, 834, 894
Hpy8I GTNNAC 3 cut(s) 50, 613, 871
HpyAV CCTTC 6 cut(s) 36, 249, 298, 417, 444, 749
HpyCH4III ACNGT 2 cut(s) 610, 1012
HpyCH4IV ACGT 1 cut(s) 1116
HpyCH4V TGCA 6 cut(s) 71, 141, 911, 920, 1068, 1120
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 147, 284, 338, 917
HpyF3I CTNAG 4 cut(s) 348, 438, 792, 1077
HpySE526I ACGT 1 cut(s) 1116
Hsp92II CATG 4 cut(s) 337, 428, 734, 937
HspAI GCGC 1 cut(s) 647
Kpn2I TCCGGA 2 cut(s) 389, 833
KspAI GTTAAC 1 cut(s) 613
Kzo9I GATC 3 cut(s) 143, 796, 1126
LmnI GCTCC 3 cut(s) 181, 836, 896
Lsp1109I GCAGC 2 cut(s) 17, 271
LweI GCATC 5 cut(s) 80, 163, 587, 850, 908
MaeI CTAG 2 cut(s) 669, 810
MaeII ACGT 1 cut(s) 1116
MaeIII GTNAC 2 cut(s) 604, 619
MalI GATC 3 cut(s) 145, 798, 1128
MboI GATC 3 cut(s) 143, 796, 1126
MboII GAAGA 9 cut(s) 277, 455, 949, 961, 982, 1001, 1070, 1148, 1182
MflI RGATCY 2 cut(s) 143, 796
MluCI AATT 6 cut(s) 131, 420, 756, 921, 1061, 1095
MlyI GAGTC 2 cut(s) 321, 596
MmeI TCCRAC 2 cut(s) 132, 1028
MnlI CCTC 9 cut(s) 137, 206, 364, 543, 723, 798, 847, 896, 1117
MroI TCCGGA 2 cut(s) 389, 833
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 8 cut(s) 102, 255, 419, 612, 660, 707, 1155, 1189
MspA1I CMGCKG 1 cut(s) 150
MspCI CTTAAG 1 cut(s) 1154
MspI CCGG 5 cut(s) 297, 390, 650, 834, 903
MspR9I CCNGG 2 cut(s) 155, 635
MvaI CCWGG 2 cut(s) 155, 635
MvnI CGCG 2 cut(s) 491, 1131
MwoI GCNNNNNNNGC 5 cut(s) 14, 147, 284, 338, 917
NcoI CCATGG 1 cut(s) 333
NdeII GATC 3 cut(s) 143, 796, 1126
NheI GCTAGC 1 cut(s) 668
NlaIII CATG 4 cut(s) 337, 428, 734, 937
NlaIV GGNNCC 2 cut(s) 295, 798
NmeAIII GCCGAG 1 cut(s) 489
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 1 cut(s) 734
PaeI GCATGC 1 cut(s) 734
PaqCI CACCTGC 1 cut(s) 146
PceI AGGCCT 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 344, 386, 1073
PflFI GACNNNGTC 1 cut(s) 234
PkrI GCNGC 4 cut(s) 7, 152, 286, 1133
PleI GAGTC 2 cut(s) 321, 595
PmaCI CACGTG 1 cut(s) 1117
PmlI CACGTG 1 cut(s) 1117
PpsI GAGTC 2 cut(s) 321, 595
Ppu21I YACGTR 1 cut(s) 1117
PshAI GACNNNNGTC 1 cut(s) 1010
PshBI ATTAAT 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 153, 633
PspCI CACGTG 1 cut(s) 1117
PspGI CCWGG 2 cut(s) 153, 633
PspN4I GGNNCC 2 cut(s) 295, 798
PspPI GGNCC 3 cut(s) 230, 836, 930
PstI CTGCAG 1 cut(s) 143
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 2 cut(s) 143, 796
PsyI GACNNNGTC 1 cut(s) 234
SaqAI TTAA 8 cut(s) 102, 255, 419, 612, 660, 707, 1155, 1189
SatI GCNGC 4 cut(s) 6, 151, 285, 1132
Sau3AI GATC 3 cut(s) 143, 796, 1126
Sau96I GGNCC 3 cut(s) 230, 836, 930
SchI GAGTC 2 cut(s) 321, 596
ScrFI CCNGG 2 cut(s) 155, 635
SfaNI GCATC 5 cut(s) 80, 163, 587, 850, 908
SfcI CTRYAG 2 cut(s) 139, 871
SinI GGWCC 2 cut(s) 230, 836
SmlI CTYRAG 2 cut(s) 802, 1154
SmoI CTYRAG 2 cut(s) 802, 1154
SphI GCATGC 1 cut(s) 734
Sse9I AATT 6 cut(s) 131, 420, 756, 921, 1061, 1095
SseBI AGGCCT 1 cut(s) 353
SsiI CCGC 3 cut(s) 150, 716, 1131
SspI AATATT 1 cut(s) 786
SspMI CTAG 2 cut(s) 669, 810
StuI AGGCCT 1 cut(s) 353
StyD4I CCNGG 2 cut(s) 153, 633
StyI CCWWGG 2 cut(s) 333, 582
TaaI ACNGT 2 cut(s) 610, 1012
TaiI ACGT 1 cut(s) 1119
TasI AATT 6 cut(s) 131, 420, 756, 921, 1061, 1095
TauI GCSGC 2 cut(s) 153, 1134
TfiI GAWTC 3 cut(s) 344, 386, 1073
Tru1I TTAA 8 cut(s) 102, 255, 419, 612, 660, 707, 1155, 1189
Tru9I TTAA 8 cut(s) 102, 255, 419, 612, 660, 707, 1155, 1189
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 2 cut(s) 5, 284
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 8 cut(s) 261, 413, 578, 583, 863, 950, 983, 1002
TspRI CASTG 1 cut(s) 364
Tth111I GACNNNGTC 1 cut(s) 234
Vha464I CTTAAG 1 cut(s) 1154
VpaK11BI GGWCC 2 cut(s) 230, 836
VspI ATTAAT 1 cut(s) 419
XagI CCTNNNNNAGG 1 cut(s) 1104
XapI RAATTY 3 cut(s) 131, 1061, 1095
XceI RCATGY 1 cut(s) 734
XmiI GTMKAC 1 cut(s) 870
XmnI GAANNNNTTC 1 cut(s) 220
XspI CTAG 2 cut(s) 669, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.