Rh6DG253800

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013164
Physical Location & Seq
Forward (+)
44276133 .. 44279529
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG253800.1

Sequence Viewer

Length: 858 bp
ATGGGTGCTGATTCTCAGGCAGCTGCTGATGTTTTCAGATGGTTTTGCGTTGAATCCGGAAGTGCTGATAATCCTTCAGTTATACTGATTCATGGCTTACCTTCTCAGGCGTACTCTTACCGGAAGGTTCTTCCGATACTTTCTAAAGCCTATCATGCCATTGCTTTTGATTGGTTAGGCTTTGGATTTTCAGATAAACCACAACCCAAGTATGGCTTTGACTATACTCTCAACGAGTATGTGACATCCTTGGAGTCCTTTATCAATGAAGTTGCTGTTGGAAAGGTCTCACTCGTTGTCCAGGGATACTTTGCACCGGCAGCTGTTAAATATGCCAGCACTCATCAGGAAAAGATTGACAACATAATACTCTTAAATCCCCCTCTAACAGAAAAGCATGCAAACCTTCCCTCAACCTTGTCCATATTCAGCAACTTTTTGTTAGGTGAAATCTTCTCTCAGGATCCTCTAAGGGCAAGTGATAAAACCTTGACAAGTTGTGGACCTTATCAAATGAAAGAGGATGATGCCATGGTTTATAGAAATCCTTACCTCACATCTGGAGCTTCTGGTTTTGCATTAAATGCAATCAGTAGGGACATGAAGAAAGAGTTGAAGAAATATGTGGAAGAAATGAGGACAATGCTAACAGATGAAAATTGGAAAGTTCCAACTACAATATGTTGGGGCAAAAGAGACCGCTGGTTGACTTATGATGGAGTGGAAGATTTCTGCAAGGATTCAAAGCATAAGCTTATAGAAATTCCAATGGCAGGCCATCACGTCCAGGAGGATCGTGGTGAAGAACTTGGACAAATCATTTACGAAATTATCAGAAAAAGGCGATTTGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

285

Amino Acids

32.27

Weight (kDa)

6.0

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 25 - 265 9.5e-20 alpha/beta hydrolase fold
Hydrolase_4 PF12146 26 - 261 3e-09 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 27 - 270 1.3e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12830
fragaria_vesca FvH4_2g17500 FvH4_2g17500
malus_domestica MD10G1148300.v1.1
prunus_persica Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1
pyrus_communis pycom05g14810 pycom10g12830
rosa_chinensis RchiOBHm_Chr6g0282231
rosa_laevigata RLG00000012899
rosa_multiflora Rmu_sc0014549.1_g000002
rosa_roxburghii Rroxscaffold_7G00186380
rosa_rugosa Rorug06G0146500
rosa_samantha Rh6BG262900 Rh6CG261800 Rh6DG253800
rosa_wichuraiana Rw6G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 1 cut(s) 700
AclWI GGATC 3 cut(s) 458, 471, 801
AcsI RAATTY 1 cut(s) 762
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 212, 773
AgsI TTSAA 3 cut(s) 53, 616, 744
AjiI CACGTC 1 cut(s) 784
AjnI CCWGG 2 cut(s) 300, 786
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
AluBI AGCT 4 cut(s) 23, 323, 566, 754
AluI AGCT 4 cut(s) 23, 323, 566, 754
Alw26I GTCTC 2 cut(s) 292, 690
AlwI GGATC 3 cut(s) 458, 471, 801
AlwNI CAGNNNCTG 1 cut(s) 26
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 1 cut(s) 775
ApeKI GCWGC 3 cut(s) 20, 23, 320
ApoI RAATTY 1 cut(s) 762
AspS9I GGNCC 1 cut(s) 503
AsuHPI GGTGA 2 cut(s) 458, 812
AvaII GGWCC 1 cut(s) 503
BamHI GGATCC 1 cut(s) 463
BbvI GCAGC 3 cut(s) 10, 32, 332
BccI CCATC 3 cut(s) 33, 710, 786
BciT130I CCWGG 2 cut(s) 302, 788
BciVI GTATCC 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 292, 690
BfuI GTATCC 1 cut(s) 299
BisI GCNGC 3 cut(s) 21, 24, 321
BlsI GCNGC 3 cut(s) 22, 25, 322
Bme1390I CCNGG 2 cut(s) 302, 788
Bme18I GGWCC 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 784
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 2 cut(s) 302, 788
BmsI GCATC 1 cut(s) 517
BpmI CTGGAG 1 cut(s) 582
BsaI GGTCTC 2 cut(s) 292, 690
BsaJI CCNNGG 3 cut(s) 249, 301, 531
BsaWI WCCGGW 2 cut(s) 56, 120
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 773
Bse118I RCCGGY 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 2 cut(s) 302, 788
BseDI CCNNGG 3 cut(s) 249, 301, 531
BseGI GGATG 2 cut(s) 245, 529
BseLI CCNNNNNNNGG 2 cut(s) 212, 773
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 3 cut(s) 29, 119, 473
BseXI GCAGC 3 cut(s) 10, 32, 332
BshFI GGCC 1 cut(s) 777
BsiSI CCGG 3 cut(s) 57, 121, 317
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 2 cut(s) 212, 773
BsmAI GTCTC 2 cut(s) 292, 690
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 1 cut(s) 777
Bso31I GGTCTC 2 cut(s) 292, 690
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 2 cut(s) 463, 793
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 1 cut(s) 700
BspANI GGCC 1 cut(s) 777
BspCNI CTCAG 3 cut(s) 28, 118, 472
BspEI TCCGGA 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 465
BspPI GGATC 3 cut(s) 458, 471, 801
BspTNI GGTCTC 2 cut(s) 292, 690
BsrDI GCAATG 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 316
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 3 cut(s) 249, 301, 531
BssMI GATC 2 cut(s) 463, 793
BssT1I CCWWGG 2 cut(s) 249, 531
Bst2UI CCWGG 2 cut(s) 302, 788
BstAPI GCANNNNNTGC 1 cut(s) 584
BstC8I GCNNGC 3 cut(s) 337, 399, 775
BstDEI CTNAG 4 cut(s) 15, 105, 459, 470
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 2 cut(s) 245, 529
BstKTI GATC 2 cut(s) 466, 796
BstMAI GTCTC 2 cut(s) 292, 690
BstMBI GATC 2 cut(s) 463, 793
BstMWI GCNNNNNNNGC 3 cut(s) 155, 320, 584
BstNI CCWGG 2 cut(s) 302, 788
BstNSI RCATGY 1 cut(s) 401
BstSCI CCNGG 2 cut(s) 300, 786
BstV1I GCAGC 3 cut(s) 10, 32, 332
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
BsuI GTATCC 1 cut(s) 299
BsuRI GGCC 1 cut(s) 777
BtgI CCRYGG 1 cut(s) 531
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 2 cut(s) 245, 529
Cac8I GCNNGC 3 cut(s) 337, 399, 775
CaiI CAGNNNCTG 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 503
Csp6I GTAC 1 cut(s) 112
CviAII CATG 5 cut(s) 92, 155, 398, 532, 601
CviJI RGCY 9 cut(s) 23, 96, 149, 180, 216, 323, 566, 754, 777
CviKI_1 RGCY 9 cut(s) 23, 96, 149, 180, 216, 323, 566, 754, 777
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 4 cut(s) 15, 105, 459, 470
DpnI GATC 2 cut(s) 465, 795
DpnII GATC 2 cut(s) 463, 793
Eco130I CCWWGG 2 cut(s) 249, 531
Eco31I GGTCTC 2 cut(s) 292, 690
Eco47I GGWCC 1 cut(s) 503
Eco57I CTGAAG 1 cut(s) 60
EcoRII CCWGG 2 cut(s) 300, 786
EcoT14I CCWWGG 2 cut(s) 249, 531
ErhI CCWWGG 2 cut(s) 249, 531
FaeI CATG 5 cut(s) 95, 158, 401, 535, 604
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 611
FatI CATG 5 cut(s) 91, 154, 397, 531, 600
Fnu4HI GCNGC 3 cut(s) 21, 24, 321
FokI GGATG 2 cut(s) 232, 536
Fsp4HI GCNGC 3 cut(s) 21, 24, 321
GluI GCNGC 3 cut(s) 21, 24, 321
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 1 cut(s) 777
HapII CCGG 3 cut(s) 57, 121, 317
Hin1II CATG 5 cut(s) 95, 158, 401, 535, 604
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HindIII AAGCTT 1 cut(s) 752
HinfI GANTC 5 cut(s) 11, 53, 88, 254, 740
HpaII CCGG 3 cut(s) 57, 121, 317
HphI GGTGA 2 cut(s) 458, 812
Hpy166II GTNNAC 2 cut(s) 503, 708
Hpy188I TCNGA 4 cut(s) 38, 135, 193, 836
Hpy188III TCNNGA 4 cut(s) 57, 347, 461, 561
Hpy8I GTNNAC 2 cut(s) 503, 708
HpyAV CCTTC 4 cut(s) 84, 111, 118, 416
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 5 cut(s) 314, 401, 578, 587, 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 320, 584
HpyF3I CTNAG 4 cut(s) 15, 105, 459, 470
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 5 cut(s) 95, 158, 401, 535, 604
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 2 cut(s) 463, 793
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 3 cut(s) 10, 32, 332
LweI GCATC 1 cut(s) 517
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 1 cut(s) 241
MalI GATC 2 cut(s) 465, 795
MboI GATC 2 cut(s) 463, 793
MboII GAAGA 7 cut(s) 122, 445, 616, 628, 641, 737, 815
MflI RGATCY 1 cut(s) 463
MluCI AATT 3 cut(s) 658, 762, 828
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 2 cut(s) 259, 695
MnlI CCTC 7 cut(s) 393, 421, 477, 514, 563, 630, 784
MroI TCCGGA 1 cut(s) 56
MseI TTAA 3 cut(s) 327, 374, 581
MspA1I CMGCKG 3 cut(s) 23, 323, 702
MspI CCGG 3 cut(s) 57, 121, 317
MspR9I CCNGG 2 cut(s) 302, 788
MvaI CCWGG 2 cut(s) 302, 788
MwoI GCNNNNNNNGC 3 cut(s) 155, 320, 584
NcoI CCATGG 1 cut(s) 531
NdeII GATC 2 cut(s) 463, 793
NlaIII CATG 5 cut(s) 95, 158, 401, 535, 604
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 1 cut(s) 401
PaeI GCATGC 1 cut(s) 401
PfeI GAWTC 4 cut(s) 11, 53, 88, 740
PfoI TCCNGGA 1 cut(s) 786
PkrI GCNGC 3 cut(s) 22, 25, 322
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp6I CCWGG 2 cut(s) 300, 786
PspGI CCWGG 2 cut(s) 300, 786
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 503
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 1 cut(s) 463
PvuII CAGCTG 2 cut(s) 23, 323
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SaqAI TTAA 3 cut(s) 327, 374, 581
SatI GCNGC 3 cut(s) 21, 24, 321
Sau3AI GATC 2 cut(s) 463, 793
Sau96I GGNCC 1 cut(s) 503
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 2 cut(s) 302, 788
SfaNI GCATC 1 cut(s) 517
SinI GGWCC 1 cut(s) 503
SphI GCATGC 1 cut(s) 401
Sse9I AATT 3 cut(s) 658, 762, 828
SsiI CCGC 1 cut(s) 700
StyD4I CCNGG 2 cut(s) 300, 786
StyI CCWWGG 2 cut(s) 249, 531
TaiI ACGT 1 cut(s) 786
TasI AATT 3 cut(s) 658, 762, 828
TfiI GAWTC 4 cut(s) 11, 53, 88, 740
Tru1I TTAA 3 cut(s) 327, 374, 581
Tru9I TTAA 3 cut(s) 327, 374, 581
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 3 cut(s) 20, 23, 320
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 5 cut(s) 80, 282, 530, 617, 669
VpaK11BI GGWCC 1 cut(s) 503
XapI RAATTY 1 cut(s) 762
XceI RCATGY 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.