Rw6G022460

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013164
Physical Location & Seq
Forward (+)
44358830 .. 44361595
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022460.1

Sequence Viewer

Length: 1185 bp
ATGGCTACCCAAGCCAAAACCCCTTCTCTGTGTTACACTCACAAACCGCAATTCTTTCTCCATAATCCTATTCCTGCGTCCATTTCACTTTTTGGTAATGGCAAGGGCCAAAAGAAGCCTCTCAATCTCAGTTGCAGCTCCAACGGCGATGATGATTATTACATAGATGCTGATTATCAAGTGGGTGACGGCTTCTCTTTCAGTGGAGGAAAGTATTCAGATGGACCTAGCCCGTCTGATGAATGGTTCAAGCAAGGGAAGATAGTGAAAGCTTATACTTCTCTTGGTTCCGGTGAGAAGGCAAAAGATCCTATTTTTGGACTTGCAATGGGTGCTGATTCTCAGGCAGCTGCTGATGTTTTCAGATGGTTTTGCGTTGAATCCGGAAGTGCTGATAATCCTTCAGTTATACTGATTCATGGCTTACCTTCTCAGGCGTACTCTTACCGCAAGGTTCTTCCGATACTTTCTAAAGCCTATCATGCCATTGCTTTTGATTGGTTAGGCTTTGGATTTTCAGATAAACCACAACCCAAGTATGGCTTTGACTATACTCTCAACGAGTATGTGACATCCTTGGAGTCCTTTATCAATGAAGTTGCTGTCGGAAAGGTCTCACTCGTTGTCCAGGGATACTTTGCGCCGGCAGCTGTTAAATATGCCAGCACTCATCAGGAAAAGATTGACAACATAATACTCTTAAATCCCCCTCTAACAGCAAAGCATGCAAACCTTCCCTCAACCTTGTCCATATTCAGCAACTTTTTGTTAGGTGAAATCTTCTCTCAGGATCCTCTAAGGGCAAGTGATAAAACCTTGACAAGTTGTGGACCTTATCAAATGAAAGAGGATGATGCCATGGTTTATAGAAATCCTTACCTCACATCTGGAGCTTCTGGTTTTGCATTAAATGCAATCAGTAGGGACATGAAGAAAGAGTTGAAGAAATATGTGGAAGAAATGAGGACAATGCTAACAGATGAAAATTGGAAAGTTCCAACTACAATATGTTGGGGCAAAAGAGACCGCTGGTTGACTTATGATGGAGTGGAAGATTTCTGCAAGGATTCAAAGCATAAGCTTATAGAAATTCCAATGGCAGGCCATCACGTCCAGGAGGATCGTGGTGAAGAACTTGGACAAATCATTTACGAAATTATCAGAAAAAGGAGATTTGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

394

Amino Acids

43.93

Weight (kDa)

6.32

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 134 - 374 2.1e-19 alpha/beta hydrolase fold
Hydrolase_4 PF12146 135 - 370 6.6e-09 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 136 - 379 3e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12830
fragaria_vesca FvH4_2g17500 FvH4_2g17500
malus_domestica MD10G1148300.v1.1
prunus_persica Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1
pyrus_communis pycom05g14810 pycom10g12830
rosa_chinensis RchiOBHm_Chr6g0282231
rosa_laevigata RLG00000012899
rosa_multiflora Rmu_sc0014549.1_g000002
rosa_roxburghii Rroxscaffold_7G00186380
rosa_rugosa Rorug06G0146500
rosa_samantha Rh6BG262900 Rh6CG261800 Rh6DG253800
rosa_wichuraiana Rw6G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 3 cut(s) 47, 448, 1027
AclWI GGATC 4 cut(s) 302, 785, 798, 1128
AcsI RAATTY 1 cut(s) 1089
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 3 cut(s) 317, 539, 1100
AgsI TTSAA 4 cut(s) 250, 380, 943, 1071
AjiI CACGTC 1 cut(s) 1111
AjnI CCWGG 2 cut(s) 627, 1113
AluBI AGCT 6 cut(s) 138, 272, 350, 650, 893, 1081
AluI AGCT 6 cut(s) 138, 272, 350, 650, 893, 1081
Alw26I GTCTC 2 cut(s) 619, 1017
AlwI GGATC 4 cut(s) 302, 785, 798, 1128
AlwNI CAGNNNCTG 1 cut(s) 353
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 2 cut(s) 106, 1102
ApeKI GCWGC 4 cut(s) 135, 347, 350, 647
ApoI RAATTY 1 cut(s) 1089
Asp700I GAANNNNTTC 1 cut(s) 214
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 3 cut(s) 106, 224, 830
AsuHPI GGTGA 4 cut(s) 197, 305, 785, 1139
AvaII GGWCC 2 cut(s) 224, 830
BamHI GGATCC 1 cut(s) 790
BbvI GCAGC 4 cut(s) 147, 337, 359, 659
BccI CCATC 4 cut(s) 215, 360, 1037, 1113
BceAI ACGGC 2 cut(s) 160, 205
BciT130I CCWGG 2 cut(s) 629, 1115
BciVI GTATCC 1 cut(s) 626
BcoDI GTCTC 2 cut(s) 619, 1017
BfaI CTAG 1 cut(s) 228
BfuI GTATCC 1 cut(s) 626
BisI GCNGC 4 cut(s) 136, 348, 351, 648
BlsI GCNGC 4 cut(s) 137, 349, 352, 649
Bme1390I CCNGG 2 cut(s) 629, 1115
Bme18I GGWCC 2 cut(s) 224, 830
BmgBI CACGTC 1 cut(s) 1111
BmgT120I GGNCC 3 cut(s) 106, 224, 830
BmiI GGNNCC 2 cut(s) 289, 792
BmrFI CCNGG 2 cut(s) 629, 1115
BmsI GCATC 2 cut(s) 157, 844
BpmI CTGGAG 1 cut(s) 909
BsaI GGTCTC 2 cut(s) 619, 1017
BsaJI CCNNGG 3 cut(s) 576, 628, 858
BsaWI WCCGGW 2 cut(s) 290, 383
BsaXI ACNNNNNCTCC 1 cut(s) 1162
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 539, 1100
Bse118I RCCGGY 1 cut(s) 643
Bse3DI GCAATG 2 cut(s) 333, 486
BseAI TCCGGA 1 cut(s) 383
BseBI CCWGG 2 cut(s) 629, 1115
BseDI CCNNGG 3 cut(s) 576, 628, 858
BseGI GGATG 2 cut(s) 572, 856
BseLI CCNNNNNNNGG 3 cut(s) 317, 539, 1100
BseMI GCAATG 2 cut(s) 333, 486
BseMII CTCAG 4 cut(s) 142, 356, 446, 800
BseXI GCAGC 4 cut(s) 147, 337, 359, 659
BshFI GGCC 2 cut(s) 108, 1104
BsiSI CCGG 3 cut(s) 291, 384, 644
BslFI GGGAC 1 cut(s) 938
BslI CCNNNNNNNGG 3 cut(s) 317, 539, 1100
BsmAI GTCTC 2 cut(s) 619, 1017
BsmFI GGGAC 1 cut(s) 938
BsnI GGCC 2 cut(s) 108, 1104
Bso31I GGTCTC 2 cut(s) 619, 1017
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 3 cut(s) 307, 790, 1120
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 3 cut(s) 47, 448, 1027
BspANI GGCC 2 cut(s) 108, 1104
BspCNI CTCAG 4 cut(s) 141, 355, 445, 799
BspEI TCCGGA 1 cut(s) 383
BspLI GGNNCC 2 cut(s) 289, 792
BspPI GGATC 4 cut(s) 302, 785, 798, 1128
BspTNI GGTCTC 2 cut(s) 619, 1017
BsrDI GCAATG 2 cut(s) 333, 486
BsrFI RCCGGY 1 cut(s) 643
BssAI RCCGGY 1 cut(s) 643
BssECI CCNNGG 3 cut(s) 576, 628, 858
BssMI GATC 3 cut(s) 307, 790, 1120
BssT1I CCWWGG 2 cut(s) 576, 858
Bst2UI CCWGG 2 cut(s) 629, 1115
BstAPI GCANNNNNTGC 3 cut(s) 332, 725, 911
BstC8I GCNNGC 4 cut(s) 645, 664, 726, 1102
BstDEI CTNAG 5 cut(s) 128, 342, 432, 786, 797
BstDSI CCRYGG 1 cut(s) 858
BstF5I GGATG 2 cut(s) 572, 856
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 3 cut(s) 310, 793, 1123
BstMAI GTCTC 2 cut(s) 619, 1017
BstMBI GATC 3 cut(s) 307, 790, 1120
BstMWI GCNNNNNNNGC 7 cut(s) 11, 144, 332, 482, 647, 725, 911
BstNI CCWGG 2 cut(s) 629, 1115
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 2 cut(s) 627, 1113
BstV1I GCAGC 4 cut(s) 147, 337, 359, 659
BstX2I RGATCY 2 cut(s) 307, 790
BstYI RGATCY 2 cut(s) 307, 790
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 2 cut(s) 108, 1104
BtgI CCRYGG 1 cut(s) 858
BtgZI GCGATG 1 cut(s) 162
BtrI CACGTC 1 cut(s) 1111
BtsCI GGATG 2 cut(s) 572, 856
BtsIMutI CAGTG 1 cut(s) 208
Cac8I GCNNGC 4 cut(s) 645, 664, 726, 1102
CaiI CAGNNNCTG 1 cut(s) 353
CfoI GCGC 1 cut(s) 643
Cfr10I RCCGGY 1 cut(s) 643
Cfr13I GGNCC 3 cut(s) 106, 224, 830
CseI GACGC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 439
CviAII CATG 5 cut(s) 419, 482, 725, 859, 928
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 5 cut(s) 128, 342, 432, 786, 797
DpnI GATC 3 cut(s) 309, 792, 1122
DpnII GATC 3 cut(s) 307, 790, 1120
Eco130I CCWWGG 2 cut(s) 576, 858
Eco31I GGTCTC 2 cut(s) 619, 1017
Eco47I GGWCC 2 cut(s) 224, 830
Eco57I CTGAAG 1 cut(s) 387
EcoRII CCWGG 2 cut(s) 627, 1113
EcoT14I CCWWGG 2 cut(s) 576, 858
ErhI CCWWGG 2 cut(s) 576, 858
FaeI CATG 5 cut(s) 422, 485, 728, 862, 931
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 1 cut(s) 938
FatI CATG 5 cut(s) 418, 481, 724, 858, 927
Fnu4HI GCNGC 4 cut(s) 136, 348, 351, 648
FokI GGATG 2 cut(s) 559, 863
Fsp4HI GCNGC 4 cut(s) 136, 348, 351, 648
FspBI CTAG 1 cut(s) 228
GlaI GCGC 1 cut(s) 642
GluI GCNGC 4 cut(s) 136, 348, 351, 648
GsuI CTGGAG 1 cut(s) 909
HaeIII GGCC 2 cut(s) 108, 1104
HapII CCGG 3 cut(s) 291, 384, 644
HgaI GACGC 1 cut(s) 66
HhaI GCGC 1 cut(s) 643
Hin1II CATG 5 cut(s) 422, 485, 728, 862, 931
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HincII GTYRAC 1 cut(s) 1035
HindII GTYRAC 1 cut(s) 1035
HindIII AAGCTT 2 cut(s) 270, 1079
HinfI GANTC 5 cut(s) 338, 380, 415, 581, 1067
HpaII CCGG 3 cut(s) 291, 384, 644
HphI GGTGA 4 cut(s) 197, 305, 785, 1139
Hpy166II GTNNAC 2 cut(s) 830, 1035
Hpy188I TCNGA 7 cut(s) 220, 238, 365, 462, 520, 608, 1163
Hpy188III TCNNGA 4 cut(s) 384, 674, 788, 888
Hpy8I GTNNAC 2 cut(s) 830, 1035
HpyAV CCTTC 5 cut(s) 33, 292, 411, 438, 743
HpyCH4IV ACGT 1 cut(s) 1110
HpyCH4V TGCA 6 cut(s) 135, 326, 728, 905, 914, 1062
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 144, 332, 482, 647, 725, 911
HpyF3I CTNAG 5 cut(s) 128, 342, 432, 786, 797
HpySE526I ACGT 1 cut(s) 1110
Hsp92II CATG 5 cut(s) 422, 485, 728, 862, 931
HspAI GCGC 1 cut(s) 641
Kpn2I TCCGGA 1 cut(s) 383
KroI GCCGGC 1 cut(s) 643
KroNI GCCGGC 1 cut(s) 645
Kzo9I GATC 3 cut(s) 307, 790, 1120
LmnI GCTCC 2 cut(s) 143, 890
Lsp1109I GCAGC 4 cut(s) 147, 337, 359, 659
LweI GCATC 2 cut(s) 157, 844
MaeI CTAG 1 cut(s) 228
MaeII ACGT 1 cut(s) 1110
MaeIII GTNAC 3 cut(s) 32, 185, 568
MalI GATC 3 cut(s) 309, 792, 1122
MboI GATC 3 cut(s) 307, 790, 1120
MboII GAAGA 8 cut(s) 271, 449, 772, 943, 955, 968, 1064, 1142
MflI RGATCY 2 cut(s) 307, 790
MluCI AATT 4 cut(s) 50, 985, 1089, 1155
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 3 cut(s) 165, 586, 1022
MnlI CCTC 9 cut(s) 129, 200, 720, 748, 804, 841, 890, 957, 1111
MroI TCCGGA 1 cut(s) 383
MroNI GCCGGC 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 3 cut(s) 654, 701, 908
MspA1I CMGCKG 3 cut(s) 350, 650, 1029
MspI CCGG 3 cut(s) 291, 384, 644
MspR9I CCNGG 2 cut(s) 629, 1115
MvaI CCWGG 2 cut(s) 629, 1115
MwoI GCNNNNNNNGC 7 cut(s) 11, 144, 332, 482, 647, 725, 911
NaeI GCCGGC 1 cut(s) 645
NcoI CCATGG 1 cut(s) 858
NdeII GATC 3 cut(s) 307, 790, 1120
NgoMIV GCCGGC 1 cut(s) 643
NlaIII CATG 5 cut(s) 422, 485, 728, 862, 931
NlaIV GGNNCC 2 cut(s) 289, 792
NmuCI GTSAC 2 cut(s) 185, 568
NspI RCATGY 1 cut(s) 728
PaeI GCATGC 1 cut(s) 728
PdiI GCCGGC 1 cut(s) 645
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 4 cut(s) 338, 380, 415, 1067
PfoI TCCNGGA 1 cut(s) 1113
PkrI GCNGC 4 cut(s) 137, 349, 352, 649
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
Psp6I CCWGG 2 cut(s) 627, 1113
PspGI CCWGG 2 cut(s) 627, 1113
PspN4I GGNNCC 2 cut(s) 289, 792
PspPI GGNCC 3 cut(s) 106, 224, 830
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 2 cut(s) 307, 790
PvuII CAGCTG 2 cut(s) 350, 650
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SaqAI TTAA 3 cut(s) 654, 701, 908
SatI GCNGC 4 cut(s) 136, 348, 351, 648
Sau3AI GATC 3 cut(s) 307, 790, 1120
Sau96I GGNCC 3 cut(s) 106, 224, 830
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 2 cut(s) 629, 1115
SfaNI GCATC 2 cut(s) 157, 844
SinI GGWCC 2 cut(s) 224, 830
SphI GCATGC 1 cut(s) 728
Sse9I AATT 4 cut(s) 50, 985, 1089, 1155
SsiI CCGC 3 cut(s) 47, 448, 1027
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 627, 1113
StyI CCWWGG 2 cut(s) 576, 858
TaiI ACGT 1 cut(s) 1113
TasI AATT 4 cut(s) 50, 985, 1089, 1155
TfiI GAWTC 4 cut(s) 338, 380, 415, 1067
Tru1I TTAA 3 cut(s) 654, 701, 908
Tru9I TTAA 3 cut(s) 654, 701, 908
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 2 cut(s) 185, 568
TseI GCWGC 4 cut(s) 135, 347, 350, 647
Tsp45I GTSAC 2 cut(s) 185, 568
TspDTI ATGAA 6 cut(s) 255, 407, 609, 857, 944, 996
TspRI CASTG 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 224, 830
XapI RAATTY 1 cut(s) 1089
XceI RCATGY 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 1121
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.