Rorug06G0146500

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013164
Physical Location & Seq
Forward (+)
20919692 .. 20922616
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0146500.1

Sequence Viewer

Length: 1017 bp
ATGAAACAAGATATGCAGAGTGATAATTTTAACATGTTTGACCTTATTGCATATCGTATCAAGTTTACTCTCCTCATCCGCACGCTGGAGACAAGTGGTGTATCTATCAAAATTACGATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATTTACCCATTTGCTGTGCACTTTAGATTTTGAACACATCGTGCTTCATACTACTGGCTATTGCATTCTCTGGGCCTATATCAACCACATGGGCTTGACGTCGACTTCCATTAATAAGTTTGTTAGAGGAATTCGAATCACCAGAAGTGATTGTAGTATGATTCATCTGAGTTGCCTCGAGTACCACATCAGAGAAGAGCTGAATAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCTTGTAAAGGTCGTCATTACCAACTGGAATGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGACTCTGAGGCGGAGTTCTACAAGTTTGTGGTACTTCTGATTGTCACTGGCTTATGTTTTCTGGCCTACACATCAGTTCCATTTTCCAAGGTGTTGTACATTGAGTGCTCCAATTTTTGCCTTAAAGACTCAAAATACTACTTCGGACTTGCTCCTGGAGAATCTGCCCTGCTCAGACACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTGTTTTAGCTGAGGATAATGAGACTGTCCTTGAGATACAGGTCGAGTATGATCAGGAGAAGAAGACAAAGCCAAAGGGGGTGGTGCATTGGGTCACTGAACCTTCTCCTGTGGTCGATCCACTCAAAGTGGAAGTCCAATTGTTTGACAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATTGAAAGTGGTAATACCAAATGCATTTGCTGTCCCCCCACTTGGGAAAGGTGTTGGTGTTGGCTATTTGACTCCTGAGAAGCTTGTGTTTAATACGGTCACACTGCGAGATAGCTACAAGAGTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

338

Amino Acids

38.55

Weight (kDa)

6.09

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12830
fragaria_vesca FvH4_2g17500 FvH4_2g17500
malus_domestica MD10G1148300.v1.1
prunus_persica Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1 Prupe.8G187300_v2.0.a1
pyrus_communis pycom05g14810 pycom10g12830
rosa_chinensis RchiOBHm_Chr6g0282231
rosa_laevigata RLG00000012899
rosa_multiflora Rmu_sc0014549.1_g000002
rosa_roxburghii Rroxscaffold_7G00186380
rosa_rugosa Rorug06G0146500
rosa_samantha Rh6BG262900 Rh6CG261800 Rh6DG253800
rosa_wichuraiana Rw6G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 642
AatII GACGTC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 254
AciI CCGC 2 cut(s) 79, 470
AclWI GGATC 1 cut(s) 785
AcsI RAATTY 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 251
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 3 cut(s) 335, 492, 557
AfiI CCNNNNNNNGG 4 cut(s) 85, 433, 926, 927
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 3 cut(s) 185, 832, 889
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 6 cut(s) 352, 365, 683, 853, 967, 999
AluI AGCT 6 cut(s) 352, 365, 683, 853, 967, 999
Alw21I GWGCWC 2 cut(s) 173, 569
Alw26I GTCTC 2 cut(s) 83, 689
Alw44I GTGCAC 1 cut(s) 169
AlwI GGATC 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 3 cut(s) 225, 455, 522
ApaLI GTGCAC 1 cut(s) 169
ApoI RAATTY 1 cut(s) 282
ArsI GACNNNNNNTTYG 2 cut(s) 792, 824
AseI ATTAAT 1 cut(s) 264
AspS9I GGNCC 2 cut(s) 225, 456
AsuHPI GGTGA 1 cut(s) 283
AsuII TTCGAA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 743
Bbv12I GWGCWC 2 cut(s) 173, 569
BbvCI CCTCAGC 1 cut(s) 684
BciT130I CCWGG 1 cut(s) 615
BclI TGATCA 1 cut(s) 724
BcoDI GTCTC 2 cut(s) 83, 689
BlpI GCTNAGC 1 cut(s) 849
Bme1390I CCNGG 1 cut(s) 615
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 2 cut(s) 225, 456
BmrFI CCNGG 1 cut(s) 615
BmsI GCATC 1 cut(s) 652
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 2 cut(s) 107, 636
Bpu10I CCTNAGC 1 cut(s) 684
Bpu1102I GCTNAGC 1 cut(s) 849
Bpu14I TTCGAA 1 cut(s) 286
BpuEI CTTGAG 2 cut(s) 166, 725
BsaHI GRCGYC 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 546
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 433, 926, 927
Bse1I ACTGG 4 cut(s) 211, 419, 511, 866
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 3 cut(s) 75, 667, 676
BseLI CCNNNNNNNGG 4 cut(s) 85, 433, 926, 927
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 7 cut(s) 311, 456, 646, 675, 840, 849, 951
BseNI ACTGG 4 cut(s) 211, 419, 511, 866
BseRI GAGGAG 2 cut(s) 62, 357
BseSI GKGCMC 1 cut(s) 173
BshFI GGCC 3 cut(s) 227, 457, 524
BsiHKAI GWGCWC 2 cut(s) 173, 569
BsiHKCI CYCGRG 1 cut(s) 329
BslFI GGGAC 1 cut(s) 902
BslI CCNNNNNNNGG 4 cut(s) 85, 433, 926, 927
BsmAI GTCTC 2 cut(s) 83, 689
BsmFI GGGAC 1 cut(s) 902
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 3 cut(s) 227, 457, 524
BsoBI CYCGRG 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 286
Bsp1286I GDGCHC 2 cut(s) 173, 569
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 4 cut(s) 388, 724, 790, 871
Bsp1720I GCTNAGC 1 cut(s) 849
BspACI CCGC 2 cut(s) 79, 470
BspANI GGCC 3 cut(s) 227, 457, 524
BspCNI CTCAG 7 cut(s) 312, 457, 645, 676, 841, 848, 952
BspPI GGATC 1 cut(s) 785
BspQI GCTCTTC 1 cut(s) 342
BspT104I TTCGAA 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 126
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 4 cut(s) 211, 419, 511, 866
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 4 cut(s) 388, 724, 790, 871
BssNI GRCGYC 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 3 cut(s) 440, 700, 982
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 555
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 7 cut(s) 320, 465, 632, 684, 835, 849, 960
BstENI CCTNNNNNAGG 1 cut(s) 431
BstF5I GGATG 3 cut(s) 75, 667, 676
BstKTI GATC 4 cut(s) 391, 727, 793, 874
BstMAI GTCTC 2 cut(s) 83, 689
BstMBI GATC 4 cut(s) 388, 724, 790, 871
BstNI CCWGG 1 cut(s) 615
BstNSI RCATGY 2 cut(s) 37, 645
BstSCI CCNGG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 173
BstV2I GAAGAC 1 cut(s) 743
BsuRI GGCC 3 cut(s) 227, 457, 524
BtsCI GGATG 3 cut(s) 75, 667, 676
BtsI GCAGTG 1 cut(s) 986
BtsIMutI CAGTG 3 cut(s) 504, 768, 986
Cac8I GCNNGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 225, 456
Csp6I GTAC 3 cut(s) 334, 491, 556
CspCI CAANNNNNGTGG 2 cut(s) 735, 770
CviAII CATG 3 cut(s) 34, 241, 642
CviQI GTAC 3 cut(s) 334, 491, 556
DdeI CTNAG 7 cut(s) 320, 465, 632, 684, 835, 849, 960
DpnI GATC 4 cut(s) 390, 726, 792, 873
DpnII GATC 4 cut(s) 388, 724, 790, 871
DraIII CACNNNGTG 1 cut(s) 193
DrdI GACNNNNNNGTC 1 cut(s) 642
DseDI GACNNNNNNGTC 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 1 cut(s) 485
Eco130I CCWWGG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 546
EcoT22I ATGCAT 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 3 cut(s) 37, 244, 645
FaqI GGGAC 1 cut(s) 902
FatI CATG 3 cut(s) 33, 240, 641
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 254
FokI GGATG 3 cut(s) 62, 674, 683
GsuI CTGGAG 2 cut(s) 107, 636
HaeIII GGCC 3 cut(s) 227, 457, 524
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 3 cut(s) 37, 244, 645
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 8 cut(s) 139, 288, 313, 461, 587, 620, 841, 955
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 3 cut(s) 66, 171, 255
Hpy188I TCNGA 9 cut(s) 321, 344, 466, 498, 605, 635, 838, 871, 876
Hpy188III TCNNGA 3 cut(s) 145, 728, 959
Hpy8I GTNNAC 3 cut(s) 66, 171, 255
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 1 cut(s) 786
HpyCH4III ACNGT 3 cut(s) 440, 700, 982
HpyCH4IV ACGT 1 cut(s) 251
HpyCH4V TGCA 8 cut(s) 16, 50, 131, 171, 216, 665, 760, 908
HpyF3I CTNAG 7 cut(s) 320, 465, 632, 684, 835, 849, 960
HpySE526I ACGT 1 cut(s) 251
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 3 cut(s) 37, 244, 645
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 4 cut(s) 388, 724, 790, 871
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 3 cut(s) 370, 572, 616
LweI GCATC 1 cut(s) 652
MaeII ACGT 1 cut(s) 251
MaeIII GTNAC 3 cut(s) 502, 766, 982
MalI GATC 4 cut(s) 390, 726, 792, 873
MboI GATC 4 cut(s) 388, 724, 790, 871
MboII GAAGA 3 cut(s) 359, 745, 748
MfeI CAATTG 1 cut(s) 812
MhlI GDGCHC 2 cut(s) 173, 569
MluCI AATT 6 cut(s) 25, 111, 282, 571, 812, 884
MlyI GAGTC 3 cut(s) 455, 581, 949
MnlI CCTC 7 cut(s) 83, 272, 338, 378, 437, 460, 679
Mph1103I ATGCAT 1 cut(s) 910
MseI TTAA 4 cut(s) 30, 264, 582, 975
MspR9I CCNGG 1 cut(s) 615
MunI CAATTG 1 cut(s) 812
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 1 cut(s) 615
NdeII GATC 4 cut(s) 388, 724, 790, 871
NlaIII CATG 3 cut(s) 37, 244, 645
NmuCI GTSAC 3 cut(s) 502, 766, 982
NsiI ATGCAT 1 cut(s) 910
NspI RCATGY 2 cut(s) 37, 645
NspV TTCGAA 1 cut(s) 286
PaeR7I CTCGAG 1 cut(s) 329
PciI ACATGT 1 cut(s) 33
PciSI GCTCTTC 1 cut(s) 342
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 5 cut(s) 139, 288, 313, 620, 841
PfoI TCCNGGA 1 cut(s) 613
PleI GAGTC 3 cut(s) 455, 581, 949
PpsI GAGTC 3 cut(s) 455, 581, 949
PscI ACATGT 1 cut(s) 33
PshBI ATTAAT 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspPI GGNCC 2 cut(s) 225, 456
PspXI VCTCGAGB 1 cut(s) 329
RsaI GTAC 3 cut(s) 335, 492, 557
RsaNI GTAC 3 cut(s) 334, 491, 556
SalI GTCGAC 1 cut(s) 253
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 4 cut(s) 30, 264, 582, 975
Sau3AI GATC 4 cut(s) 388, 724, 790, 871
Sau96I GGNCC 2 cut(s) 225, 456
SchI GAGTC 3 cut(s) 455, 581, 949
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 2 cut(s) 173, 569
SfaNI GCATC 1 cut(s) 652
Sfr274I CTCGAG 1 cut(s) 329
SfuI TTCGAA 1 cut(s) 286
SlaI CTCGAG 1 cut(s) 329
SmlI CTYRAG 3 cut(s) 145, 329, 704
SmoI CTYRAG 3 cut(s) 145, 329, 704
Sse9I AATT 6 cut(s) 25, 111, 282, 571, 812, 884
SsiI CCGC 2 cut(s) 79, 470
SspI AATATT 1 cut(s) 153
StyD4I CCNGG 1 cut(s) 613
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 3 cut(s) 440, 700, 982
TaiI ACGT 1 cut(s) 254
TaqI TCGA 5 cut(s) 254, 286, 330, 717, 789
TasI AATT 6 cut(s) 25, 111, 282, 571, 812, 884
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 5 cut(s) 139, 288, 313, 620, 841
Tru1I TTAA 4 cut(s) 30, 264, 582, 975
Tru9I TTAA 4 cut(s) 30, 264, 582, 975
TscAI CASTG 3 cut(s) 511, 775, 993
TseFI GTSAC 3 cut(s) 502, 766, 982
Tsp45I GTSAC 3 cut(s) 502, 766, 982
TspDTI ATGAA 3 cut(s) 17, 188, 305
TspRI CASTG 3 cut(s) 511, 775, 993
VneI GTGCAC 1 cut(s) 169
VspI ATTAAT 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 431
XapI RAATTY 1 cut(s) 282
XceI RCATGY 2 cut(s) 37, 645
XhoI CTCGAG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 254
ZraI GACGTC 1 cut(s) 252
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.