AT4G13200

at4g13200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012498
Physical Location & Seq
Reverse (-)
7668067 .. 7669250
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13200.1

Sequence Viewer

Length: 558 bp
ATGAGTAGCTTCACGATTCCATCTCCTTCCTCCTTCTCTCTTTCCAATTCATATAATCAAACCTCTCCTCATTCCTTCACTCTCCGCAATTCTCGTTCCAATTTTGAGTTCCACCGGTTACGATTAGATGTGGAGTCTCGAAGAAGAAGCACTTCTTTACGGTCCAATTGCTCCACCAAAGGTACAGATTCAGGTGAAAATGAGAACAAAAGTGTGTTAGATGCTTTCTTTTTGGGGAAAGCATTAGCTGAGGTTATCAATGAGAGAATTGAGTCTACTGTTGGAGAAGTGTTGAGTACTATTGGAAAGTTTCAAGCTGAGCAACAGAAACAAGTTCAAGAGATTCAGGAAGAGGTTCTTGAAAGAGCTAAGAAAGCTAAGGAAAGAGCAGCTCGAGAAACTATGGAGGAACAAGGACTTGTTGCTTCCAAACCAGTAGCCATTACTCGAAATCCGGATGCTGTTGTTGTTGCCTCTGTAACTTCTACATCGACCGTTGAGAGCAAGACTATAATGGTTGATGTAGAGGAAAGCTCGAGTTCCAGTGATGAAGACTGA

Protein Analysis

185

Amino Acids

20.43

Weight (kDa)

5.27

Isoelectric Point (pI)

61.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 70 - 159 2.4e-33 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 275
AccIII TCCGGA 1 cut(s) 454
AciI CCGC 1 cut(s) 85
AfaI GTAC 2 cut(s) 184, 298
AgeI ACCGGT 1 cut(s) 114
AgsI TTSAA 3 cut(s) 314, 338, 362
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 7 cut(s) 9, 248, 317, 368, 377, 392, 534
AluI AGCT 7 cut(s) 9, 248, 317, 368, 377, 392, 534
Alw26I GTCTC 1 cut(s) 141
Ama87I CYCGRG 2 cut(s) 393, 535
Aor13HI TCCGGA 1 cut(s) 454
ApeKI GCWGC 1 cut(s) 389
AsiGI ACCGGT 1 cut(s) 114
Asp700I GAANNNNTTC 2 cut(s) 151, 354
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 2 cut(s) 393, 535
AvaII GGWCC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 401
BccI CCATC 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 141
BisI GCNGC 1 cut(s) 390
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 391
BmcAI AGTACT 1 cut(s) 298
Bme18I GGWCC 1 cut(s) 162
BmeT110I CYCGRG 2 cut(s) 393, 535
BmgT120I GGNCC 1 cut(s) 162
BmsI GCATC 2 cut(s) 211, 448
BplI GAGNNNNNCTC 2 cut(s) 518, 550
Bpu10I CCTNAGC 2 cut(s) 249, 378
Bpu1102I GCTNAGC 1 cut(s) 318
BsaWI WCCGGW 2 cut(s) 114, 454
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 2 cut(s) 434, 543
BseAI TCCGGA 1 cut(s) 454
BseGI GGATG 1 cut(s) 463
BseMII CTCAG 2 cut(s) 240, 309
BseNI ACTGG 2 cut(s) 434, 543
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 1 cut(s) 401
Bsh1285I CGRYCG 1 cut(s) 495
BshTI ACCGGT 1 cut(s) 114
BsiEI CGRYCG 1 cut(s) 495
BsiHKCI CYCGRG 2 cut(s) 393, 535
BsiSI CCGG 2 cut(s) 115, 455
BsmAI GTCTC 1 cut(s) 141
BsoBI CYCGRG 2 cut(s) 393, 535
Bsp13I TCCGGA 1 cut(s) 454
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 1 cut(s) 85
BspCNI CTCAG 2 cut(s) 241, 310
BspEI TCCGGA 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 2 cut(s) 434, 543
BssAI RCCGGY 1 cut(s) 114
Bst4CI ACNGT 3 cut(s) 162, 280, 496
Bst6I CTCTTC 1 cut(s) 345
BstDEI CTNAG 4 cut(s) 249, 318, 369, 378
BstF5I GGATG 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 141
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 401
BtsCI GGATG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 550
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 183, 297
CspAI ACCGGT 1 cut(s) 114
CviJI RGCY 8 cut(s) 9, 248, 317, 368, 377, 392, 440, 534
CviKI_1 RGCY 8 cut(s) 9, 248, 317, 368, 377, 392, 440, 534
CviQI GTAC 2 cut(s) 183, 297
DdeI CTNAG 4 cut(s) 249, 318, 369, 378
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 162
Eco88I CYCGRG 2 cut(s) 393, 535
FaiI YATR 4 cut(s) 52, 54, 404, 512
FalI AAGNNNNNCTT 6 cut(s) 136, 168, 139, 171, 342, 374
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 1 cut(s) 390
GluI GCNGC 1 cut(s) 390
HapII CCGG 2 cut(s) 115, 455
HinfI GANTC 5 cut(s) 16, 134, 188, 272, 343
HpaII CCGG 2 cut(s) 115, 455
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 276
Hpy188III TCNNGA 7 cut(s) 13, 138, 338, 347, 359, 395, 455
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 3 cut(s) 36, 43, 85
HpyCH4III ACNGT 3 cut(s) 162, 280, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 4 cut(s) 249, 318, 369, 378
Kpn2I TCCGGA 1 cut(s) 454
LmnI GCTCC 1 cut(s) 176
LpnPI CCDG 5 cut(s) 128, 177, 332, 447, 468
Lsp1109I GCAGC 1 cut(s) 401
LweI GCATC 2 cut(s) 211, 448
MaeIII GTNAC 2 cut(s) 117, 478
MboII GAAGA 3 cut(s) 153, 156, 362
MfeI CAATTG 1 cut(s) 166
MluCI AATT 5 cut(s) 46, 88, 100, 166, 267
MlyI GAGTC 2 cut(s) 143, 281
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 8 cut(s) 40, 73, 78, 244, 346, 400, 484, 520
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 151, 354
MspI CCGG 2 cut(s) 115, 455
MunI CAATTG 1 cut(s) 166
MwoI GCNNNNNNNGC 1 cut(s) 374
PaeR7I CTCGAG 2 cut(s) 393, 535
PdmI GAANNNNTTC 2 cut(s) 151, 354
PfeI GAWTC 3 cut(s) 16, 188, 343
PinAI ACCGGT 1 cut(s) 114
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 2 cut(s) 142, 280
PpsI GAGTC 2 cut(s) 142, 280
PspPI GGNCC 1 cut(s) 162
PspXI VCTCGAGB 1 cut(s) 535
RsaI GTAC 2 cut(s) 184, 298
RsaNI GTAC 2 cut(s) 183, 297
SatI GCNGC 1 cut(s) 390
Sau96I GGNCC 1 cut(s) 162
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 2 cut(s) 143, 281
SfaNI GCATC 2 cut(s) 211, 448
Sfr274I CTCGAG 2 cut(s) 393, 535
SinI GGWCC 1 cut(s) 162
SlaI CTCGAG 2 cut(s) 393, 535
SmlI CTYRAG 2 cut(s) 393, 535
SmoI CTYRAG 2 cut(s) 393, 535
Sse9I AATT 5 cut(s) 46, 88, 100, 166, 267
SsiI CCGC 1 cut(s) 85
TaaI ACNGT 3 cut(s) 162, 280, 496
TaqI TCGA 5 cut(s) 139, 394, 448, 491, 536
TasI AATT 5 cut(s) 46, 88, 100, 166, 267
TatI WGTACW 1 cut(s) 296
TfiI GAWTC 3 cut(s) 16, 188, 343
TscAI CASTG 1 cut(s) 550
TseI GCWGC 1 cut(s) 389
TspDTI ATGAA 1 cut(s) 39
TspRI CASTG 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 162
XhoI CTCGAG 2 cut(s) 393, 535
XmiI GTMKAC 1 cut(s) 275
XmnI GAANNNNTTC 2 cut(s) 151, 354
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.