FvH4_2g19250

protein At4g13200, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012498
Physical Location & Seq
Reverse (-)
16397058 .. 16400190
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19250.t1

Sequence Viewer

Length: 954 bp
ATGTTGAATGATATCGCAACTTCTAAGTGGTCACTCTGCGGGGGAAGTGTTCGTTTCGGGTATGGAGGAGAAAAAATCTTGAACCACTTTCCCCTGCAAAGAATTGATACACCTCCCCAGTGGGGCCAATGTTGGGCATGCCAGACCCACCTAGTATCCATCGGTCTCGACCCGACCCACGTTTTCATATTCTGCGACTGTCCGAACCCCATAGATTCCTTAGATTTTGACAAGAAAAAAGCGAGAAAACAGAGAGAAAAGCAAAGAAAGAGACAGAGACATCTGAACTCTCAACTCAAAATGATTTGTGGGGTTTTAGCTTCAGCTTCCCCACAAACTTTTCCTTCTTATTCTTCCTATTCATCTCTCGTGTCTAAACCCAAAACCAGCTGTTTCAGCTATGCTTCCAGTTCTGTCAGTGTTCCCAAGGTAAGCTCCTTGGATCTGAAGCTTGAAAGGTTCGGGCTTTATGGGGTAAAGGGGTCTCGGAGAGGCAGCAGAGTTCACTGCAACACTGGACCTGGTGAGAGTGATAGCAAGAGTGTTCTGGATGCTTTTTTCTTGGGGAAAGCTCTAGCCGAAGCGCTAAATGAGCGAATTGAGTCCAGTGTTGGCGAGTTTTTGAGTACGATTGGTAGGCTGCAAGCTGAGCAACAAAAGCAAGTAGTGGAATTTCAGGCAGACGTTCTGGAAAGAGCTAAGAAAGCCAAAGAGAAAGCAGCTCGTGAGGCTGCAGAGGCACAAGGAATTGTTTCAAAACCGACTACAGAAAGTATAACACCAGTCACCGTCACCAATGTCGTTTCTTCAGTAACTCCAACAACCACCACCAGTAATGACGGTACTCCTGCTGTGAGTCAACCTTCCTCATCTGATCCAACCCCCTCAATAACTAAGAATGTACCCGGTCCTGCCAACGAGGATACATCTTCAGAGGTGCCAATTGATGATTGA

Protein Analysis

318

Amino Acids

34.34

Weight (kDa)

8.7

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 179 - 271 1.2e-33 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 937
AciI CCGC 1 cut(s) 39
AclWI GGATC 2 cut(s) 450, 869
AcsI RAATTY 1 cut(s) 671
AcuI CTGAAG 4 cut(s) 306, 467, 792, 915
AfaI GTAC 3 cut(s) 628, 844, 903
AfeI AGCGCT 1 cut(s) 585
AfiI CCNNNNNNNGG 2 cut(s) 122, 133
AgsI TTSAA 4 cut(s) 7, 82, 455, 756
AjnI CCWGG 1 cut(s) 520
Alw26I GTCTC 4 cut(s) 170, 265, 271, 489
AlwI GGATC 2 cut(s) 450, 869
Aor51HI AGCGCT 1 cut(s) 585
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 4 cut(s) 495, 640, 719, 731
ApoI RAATTY 1 cut(s) 671
Asp700I GAANNNNTTC 1 cut(s) 751
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 3 cut(s) 124, 518, 908
AsuC2I CCSGG 1 cut(s) 906
AsuHPI GGTGA 3 cut(s) 536, 778, 784
AvaII GGWCC 2 cut(s) 518, 908
BanI GGYRCC 1 cut(s) 937
BauI CACGAG 2 cut(s) 368, 723
BbvI GCAGC 4 cut(s) 507, 627, 718, 731
BccI CCATC 1 cut(s) 167
BciT130I CCWGG 1 cut(s) 522
BciVI GTATCC 2 cut(s) 166, 916
BcnI CCSGG 1 cut(s) 906
BcoDI GTCTC 4 cut(s) 170, 265, 271, 489
BfaI CTAG 2 cut(s) 152, 575
BfmI CTRYAG 2 cut(s) 732, 765
BfoI RGCGCY 1 cut(s) 587
BfuI GTATCC 2 cut(s) 166, 916
BisI GCNGC 4 cut(s) 496, 641, 720, 732
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 4 cut(s) 497, 642, 721, 733
Bme1390I CCNGG 2 cut(s) 522, 906
Bme18I GGWCC 2 cut(s) 518, 908
BmgT120I GGNCC 3 cut(s) 124, 518, 908
BmiI GGNNCC 2 cut(s) 125, 939
BmrFI CCNGG 2 cut(s) 522, 906
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 112
Bpu1102I GCTNAGC 1 cut(s) 648
BpuMI CCSGG 1 cut(s) 906
BsaI GGTCTC 2 cut(s) 170, 489
BsaJI CCNNGG 2 cut(s) 426, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 133
Bse1I ACTGG 6 cut(s) 118, 408, 520, 606, 782, 831
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 426, 438
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 2 cut(s) 122, 133
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 6 cut(s) 118, 408, 520, 606, 782, 831
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 4 cut(s) 507, 627, 718, 731
BshFI GGCC 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 937
BsiSI CCGG 1 cut(s) 906
BslI CCNNNNNNNGG 2 cut(s) 122, 133
BsmAI GTCTC 4 cut(s) 170, 265, 271, 489
BsnI GGCC 1 cut(s) 126
Bso31I GGTCTC 2 cut(s) 170, 489
Bsp143I GATC 2 cut(s) 442, 874
Bsp1720I GCTNAGC 1 cut(s) 648
BspACI CCGC 1 cut(s) 39
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 1 cut(s) 640
BspLI GGNNCC 2 cut(s) 125, 939
BspMAI CTGCAG 1 cut(s) 736
BspPI GGATC 2 cut(s) 450, 869
BspT107I GGYRCC 1 cut(s) 937
BspTNI GGTCTC 2 cut(s) 170, 489
BsrI ACTGG 6 cut(s) 118, 408, 520, 606, 782, 831
BssECI CCNNGG 2 cut(s) 426, 438
BssMI GATC 2 cut(s) 442, 874
BssSI CACGAG 2 cut(s) 368, 723
BssT1I CCWWGG 2 cut(s) 426, 438
Bst2BI CACGAG 2 cut(s) 368, 723
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 3 cut(s) 200, 790, 842
BstC8I GCNNGC 2 cut(s) 139, 645
BstDEI CTNAG 5 cut(s) 24, 220, 648, 699, 894
BstF5I GGATG 1 cut(s) 556
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 2 cut(s) 445, 877
BstMAI GTCTC 4 cut(s) 170, 265, 271, 489
BstMBI GATC 2 cut(s) 442, 874
BstMWI GCNNNNNNNGC 7 cut(s) 396, 592, 649, 658, 704, 728, 737
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 1 cut(s) 141
BstSCI CCNGG 2 cut(s) 520, 904
BstSFI CTRYAG 2 cut(s) 732, 765
BstV1I GCAGC 4 cut(s) 507, 627, 718, 731
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuI GTATCC 2 cut(s) 166, 916
BsuRI GGCC 1 cut(s) 126
BtsCI GGATG 1 cut(s) 556
BtsI GCAGTG 1 cut(s) 505
BtsIMutI CAGTG 5 cut(s) 125, 424, 505, 513, 613
Cac8I GCNNGC 2 cut(s) 139, 645
CfoI GCGC 1 cut(s) 586
Cfr13I GGNCC 3 cut(s) 124, 518, 908
CsiI ACCWGGT 1 cut(s) 520
Csp6I GTAC 3 cut(s) 627, 843, 902
CviAII CATG 1 cut(s) 138
CviQI GTAC 3 cut(s) 627, 843, 902
DdeI CTNAG 5 cut(s) 24, 220, 648, 699, 894
DpnI GATC 2 cut(s) 444, 876
DpnII GATC 2 cut(s) 442, 874
Eco130I CCWWGG 2 cut(s) 426, 438
Eco31I GGTCTC 2 cut(s) 170, 489
Eco32I GATATC 1 cut(s) 13
Eco47I GGWCC 2 cut(s) 518, 908
Eco47III AGCGCT 1 cut(s) 585
Eco57I CTGAAG 4 cut(s) 306, 467, 792, 915
EcoRII CCWGG 1 cut(s) 520
EcoRV GATATC 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 426, 438
ErhI CCWWGG 2 cut(s) 426, 438
FaeI CATG 1 cut(s) 141
FaiI YATR 7 cut(s) 63, 139, 188, 212, 402, 471, 776
FatI CATG 1 cut(s) 137
FauI CCCGC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 496, 641, 720, 732
FokI GGATG 1 cut(s) 563
Fsp4HI GCNGC 4 cut(s) 496, 641, 720, 732
FspBI CTAG 2 cut(s) 152, 575
GlaI GCGC 1 cut(s) 585
GluI GCNGC 4 cut(s) 496, 641, 720, 732
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 1 cut(s) 126
HapII CCGG 1 cut(s) 906
HhaI GCGC 1 cut(s) 586
Hin1II CATG 1 cut(s) 141
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HincII GTYRAC 1 cut(s) 860
HindII GTYRAC 1 cut(s) 860
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 3 cut(s) 215, 602, 856
HpaII CCGG 1 cut(s) 906
HphI GGTGA 3 cut(s) 536, 778, 784
Hpy166II GTNNAC 2 cut(s) 505, 860
Hpy188I TCNGA 6 cut(s) 204, 285, 447, 489, 874, 934
Hpy188III TCNNGA 5 cut(s) 79, 167, 548, 689, 725
Hpy8I GTNNAC 2 cut(s) 505, 860
HpyAV CCTTC 2 cut(s) 354, 873
HpyCH4III ACNGT 3 cut(s) 200, 790, 842
HpyCH4IV ACGT 2 cut(s) 180, 684
HpyCH4V TGCA 4 cut(s) 97, 510, 643, 734
HpyF10VI GCNNNNNNNGC 7 cut(s) 396, 592, 649, 658, 704, 728, 737
HpyF3I CTNAG 5 cut(s) 24, 220, 648, 699, 894
HpySE526I ACGT 2 cut(s) 180, 684
Hsp92II CATG 1 cut(s) 141
HspAI GCGC 1 cut(s) 584
Kzo9I GATC 2 cut(s) 442, 874
LmnI GCTCC 1 cut(s) 440
Lsp1109I GCAGC 4 cut(s) 507, 627, 718, 731
LweI GCATC 1 cut(s) 541
MabI ACCWGGT 1 cut(s) 520
MaeI CTAG 2 cut(s) 152, 575
MaeII ACGT 2 cut(s) 180, 684
MaeIII GTNAC 4 cut(s) 30, 784, 790, 811
MalI GATC 2 cut(s) 444, 876
MboI GATC 2 cut(s) 442, 874
MboII GAAGA 3 cut(s) 345, 798, 921
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 1 cut(s) 442
MluCI AATT 5 cut(s) 102, 597, 671, 747, 942
MlyI GAGTC 2 cut(s) 611, 865
MmeI TCCRAC 2 cut(s) 842, 902
MnlI CCTC 9 cut(s) 59, 123, 485, 721, 730, 877, 895, 913, 928
MroXI GAANNNNTTC 1 cut(s) 751
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 2 cut(s) 522, 906
MunI CAATTG 1 cut(s) 942
MvaI CCWGG 1 cut(s) 522
MwoI GCNNNNNNNGC 7 cut(s) 396, 592, 649, 658, 704, 728, 737
NciI CCSGG 1 cut(s) 906
NdeII GATC 2 cut(s) 442, 874
NlaIII CATG 1 cut(s) 141
NlaIV GGNNCC 2 cut(s) 125, 939
NmuCI GTSAC 3 cut(s) 30, 784, 790
NspI RCATGY 1 cut(s) 141
PaeI GCATGC 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 751
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 4 cut(s) 497, 642, 721, 733
PleI GAGTC 2 cut(s) 610, 864
PpsI GAGTC 2 cut(s) 610, 864
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 125, 939
PspPI GGNCC 3 cut(s) 124, 518, 908
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 390
RsaI GTAC 3 cut(s) 628, 844, 903
RsaNI GTAC 3 cut(s) 627, 843, 902
SatI GCNGC 4 cut(s) 496, 641, 720, 732
Sau3AI GATC 2 cut(s) 442, 874
Sau96I GGNCC 3 cut(s) 124, 518, 908
SchI GAGTC 2 cut(s) 611, 865
ScrFI CCNGG 2 cut(s) 522, 906
SexAI ACCWGGT 1 cut(s) 520
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 2 cut(s) 732, 765
SinI GGWCC 2 cut(s) 518, 908
SphI GCATGC 1 cut(s) 141
Sse9I AATT 5 cut(s) 102, 597, 671, 747, 942
SsiI CCGC 1 cut(s) 39
SspMI CTAG 2 cut(s) 152, 575
StyD4I CCNGG 2 cut(s) 520, 904
StyI CCWWGG 2 cut(s) 426, 438
TaaI ACNGT 3 cut(s) 200, 790, 842
TaiI ACGT 2 cut(s) 183, 687
TaqI TCGA 1 cut(s) 168
TaqII GACCGA 1 cut(s) 152
TasI AATT 5 cut(s) 102, 597, 671, 747, 942
TfiI GAWTC 1 cut(s) 215
TscAI CASTG 5 cut(s) 125, 424, 512, 520, 613
TseFI GTSAC 3 cut(s) 30, 784, 790
TseI GCWGC 4 cut(s) 495, 640, 719, 731
Tsp45I GTSAC 3 cut(s) 30, 784, 790
TspDTI ATGAA 2 cut(s) 175, 351
TspRI CASTG 5 cut(s) 125, 424, 512, 520, 613
VpaK11BI GGWCC 2 cut(s) 518, 908
XapI RAATTY 1 cut(s) 671
XceI RCATGY 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 751
XspI CTAG 2 cut(s) 152, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.