Rh6CG284600

protein At4g13200, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012498
Physical Location & Seq
Reverse (-)
46216180 .. 46218304
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG284600.1

Sequence Viewer

Length: 645 bp
ATGATTAGTGGGGCTTTGGCTTCAGCTTCCCCACAAACTTTTTCTTCTTCATCATCATCTCTCGTATCTAAACCCAAAACCAGCTGTTACAGCTATGCTTCCGCCTCTGTCAGTGTTGCCAGGCTAAGCTTCTTGGATCTGAAGCTTGAAAGCTTTGGACTTTTTGGAGTAAAGGGGTCGAGGAGAGGCAGCAGAGTTCACTGCACTACCGGACCTGGTGAGGGTGATAGCAAGAGTGTTCTGGATGCCTTTTTCTTGGGGAGAGCTCTAGCGGAAGCGCTAAATGAGCGAATTGAGTCCAGTGTCGGCGAGTTTTTGAGTACAATTGGTAGGCTGCAAGCTGAGCAACAAAAGCAAGTAGAGGAGTTTCAGGCAGACGTACTGGACAGAGCCAAGAAAGCTAAAGAGAAAGCAGCACGTGAGGCTGCAGAGGCACGAGGAATTGTTTCAAAATCTACCACAGAAAATGTAACACCAGTCACCGTCACCAATGGTGTTTCGCCAATAACTCCAACAACCGCCACCACTGTTGACACTCCTGCTGTCAGTCAACCTTCATCGTCTGAGCCAACCCCCACAATAGCTGAGAATGAACCGGGTCCTGCCAACGAGGATACATCTTTAGAGGTGCCAATTGATGATTGA

Protein Analysis

214

Amino Acids

22.37

Weight (kDa)

5.0

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 77 - 176 2.4e-34 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 628
AciI CCGC 3 cut(s) 102, 272, 519
AclWI GGATC 1 cut(s) 144
AcuI CTGAAG 2 cut(s) 6, 161
AcvI CACGTG 1 cut(s) 419
AfaI GTAC 2 cut(s) 322, 381
AfeI AGCGCT 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 221
AgsI TTSAA 2 cut(s) 149, 450
AjnI CCWGG 2 cut(s) 119, 214
Alw21I GWGCWC 1 cut(s) 268
AlwI GGATC 1 cut(s) 144
Aor51HI AGCGCT 1 cut(s) 279
ApeKI GCWGC 4 cut(s) 189, 334, 413, 425
Asp700I GAANNNNTTC 1 cut(s) 445
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 2 cut(s) 212, 599
AsuC2I CCSGG 1 cut(s) 597
AsuHPI GGTGA 4 cut(s) 230, 236, 472, 478
AvaII GGWCC 2 cut(s) 212, 599
BanI GGYRCC 1 cut(s) 628
BanII GRGCYC 1 cut(s) 268
BauI CACGAG 1 cut(s) 435
BbrPI CACGTG 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 4 cut(s) 201, 321, 412, 425
BciT130I CCWGG 2 cut(s) 121, 216
BciVI GTATCC 1 cut(s) 607
BcnI CCSGG 1 cut(s) 597
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 426
BfoI RGCGCY 1 cut(s) 281
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 4 cut(s) 190, 335, 414, 426
BlpI GCTNAGC 2 cut(s) 125, 342
BlsI GCNGC 4 cut(s) 191, 336, 415, 427
Bme1390I CCNGG 3 cut(s) 121, 216, 597
Bme18I GGWCC 2 cut(s) 212, 599
BmgT120I GGNCC 2 cut(s) 212, 599
BmiI GGNNCC 2 cut(s) 600, 630
BmrFI CCNGG 3 cut(s) 121, 216, 597
BmsI GCATC 1 cut(s) 235
Bpu1102I GCTNAGC 2 cut(s) 125, 342
BpuMI CCSGG 1 cut(s) 597
BsaAI YACGTR 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 3 cut(s) 300, 387, 476
BseBI CCWGG 2 cut(s) 121, 216
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 3 cut(s) 333, 555, 576
BseNI ACTGG 3 cut(s) 300, 387, 476
BseRI GAGGAG 2 cut(s) 196, 377
BseXI GCAGC 4 cut(s) 201, 321, 412, 425
BsgI GTGCAG 1 cut(s) 187
BshNI GGYRCC 1 cut(s) 628
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 2 cut(s) 210, 596
BslI CCNNNNNNNGG 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 136
Bsp1720I GCTNAGC 2 cut(s) 125, 342
BspACI CCGC 3 cut(s) 102, 272, 519
BspCNI CTCAG 3 cut(s) 334, 556, 577
BspLI GGNNCC 2 cut(s) 600, 630
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 1 cut(s) 144
BspT107I GGYRCC 1 cut(s) 628
BsrI ACTGG 3 cut(s) 300, 387, 476
BssMI GATC 1 cut(s) 136
BssSI CACGAG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 121, 216
Bst4CI ACNGT 2 cut(s) 484, 529
BstBAI YACGTR 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 4 cut(s) 125, 342, 564, 585
BstF5I GGATG 1 cut(s) 250
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 1 cut(s) 139
BstMBI GATC 1 cut(s) 136
BstMWI GCNNNNNNNGC 7 cut(s) 90, 286, 343, 352, 398, 422, 431
BstNI CCWGG 2 cut(s) 121, 216
BstSCI CCNGG 3 cut(s) 119, 214, 595
BstSFI CTRYAG 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 201, 321, 412, 425
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuI GTATCC 1 cut(s) 607
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 4 cut(s) 118, 199, 307, 525
Cac8I GCNNGC 1 cut(s) 339
CfoI GCGC 1 cut(s) 280
Cfr13I GGNCC 2 cut(s) 212, 599
CsiI ACCWGGT 1 cut(s) 214
Csp6I GTAC 2 cut(s) 321, 380
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviQI GTAC 2 cut(s) 321, 380
DdeI CTNAG 4 cut(s) 125, 342, 564, 585
DpnI GATC 1 cut(s) 138
DpnII GATC 1 cut(s) 136
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 268
Eco47I GGWCC 2 cut(s) 212, 599
Eco47III AGCGCT 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 2 cut(s) 6, 161
Eco72I CACGTG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 119, 214
EcoT38I GRGCYC 1 cut(s) 268
FaiI YATR 1 cut(s) 96
Fnu4HI GCNGC 4 cut(s) 190, 335, 414, 426
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 4 cut(s) 190, 335, 414, 426
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 279
GluI GCNGC 4 cut(s) 190, 335, 414, 426
HaeII RGCGCY 1 cut(s) 281
HapII CCGG 2 cut(s) 210, 596
HhaI GCGC 1 cut(s) 280
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 2 cut(s) 532, 551
HindII GTYRAC 2 cut(s) 532, 551
HindIII AAGCTT 3 cut(s) 127, 143, 151
HinfI GANTC 1 cut(s) 296
HpaII CCGG 2 cut(s) 210, 596
HphI GGTGA 4 cut(s) 230, 236, 472, 478
Hpy166II GTNNAC 3 cut(s) 199, 532, 551
Hpy188I TCNGA 2 cut(s) 141, 565
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 3 cut(s) 199, 532, 551
HpyAV CCTTC 1 cut(s) 564
HpyCH4III ACNGT 2 cut(s) 484, 529
HpyCH4IV ACGT 2 cut(s) 378, 418
HpyCH4V TGCA 3 cut(s) 204, 337, 428
HpyF10VI GCNNNNNNNGC 7 cut(s) 90, 286, 343, 352, 398, 422, 431
HpyF3I CTNAG 4 cut(s) 125, 342, 564, 585
HpySE526I ACGT 2 cut(s) 378, 418
HspAI GCGC 1 cut(s) 278
Kzo9I GATC 1 cut(s) 136
Lsp1109I GCAGC 4 cut(s) 201, 321, 412, 425
LweI GCATC 1 cut(s) 235
MabI ACCWGGT 1 cut(s) 214
MaeI CTAG 1 cut(s) 269
MaeII ACGT 2 cut(s) 378, 418
MaeIII GTNAC 4 cut(s) 86, 469, 478, 484
MalI GATC 1 cut(s) 138
MboI GATC 1 cut(s) 136
MboII GAAGA 2 cut(s) 36, 39
MfeI CAATTG 2 cut(s) 324, 633
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 4 cut(s) 291, 324, 441, 633
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 536
MroXI GAANNNNTTC 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 2 cut(s) 210, 596
MspR9I CCNGG 3 cut(s) 121, 216, 597
MunI CAATTG 2 cut(s) 324, 633
MvaI CCWGG 2 cut(s) 121, 216
MwoI GCNNNNNNNGC 7 cut(s) 90, 286, 343, 352, 398, 422, 431
NciI CCSGG 1 cut(s) 597
NdeII GATC 1 cut(s) 136
NlaIV GGNNCC 2 cut(s) 600, 630
NmuCI GTSAC 2 cut(s) 478, 484
PdmI GAANNNNTTC 1 cut(s) 445
PkrI GCNGC 4 cut(s) 191, 336, 415, 427
PleI GAGTC 1 cut(s) 304
PmaCI CACGTG 1 cut(s) 419
PmlI CACGTG 1 cut(s) 419
PpsI GAGTC 1 cut(s) 304
Ppu21I YACGTR 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 268
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 119, 214
PspCI CACGTG 1 cut(s) 419
PspGI CCWGG 2 cut(s) 119, 214
PspN4I GGNNCC 2 cut(s) 600, 630
PspPI GGNCC 2 cut(s) 212, 599
PspPPI RGGWCCY 1 cut(s) 599
PstI CTGCAG 1 cut(s) 430
PsuI RGATCY 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 84
RsaI GTAC 2 cut(s) 322, 381
RsaNI GTAC 2 cut(s) 321, 380
SacI GAGCTC 1 cut(s) 268
SatI GCNGC 4 cut(s) 190, 335, 414, 426
Sau3AI GATC 1 cut(s) 136
Sau96I GGNCC 2 cut(s) 212, 599
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 3 cut(s) 121, 216, 597
SduI GDGCHC 1 cut(s) 268
SexAI ACCWGGT 1 cut(s) 214
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 2 cut(s) 212, 599
Sse9I AATT 4 cut(s) 291, 324, 441, 633
SsiI CCGC 3 cut(s) 102, 272, 519
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 3 cut(s) 119, 214, 595
TaaI ACNGT 2 cut(s) 484, 529
TaiI ACGT 2 cut(s) 381, 421
TaqI TCGA 1 cut(s) 179
TasI AATT 4 cut(s) 291, 324, 441, 633
TatI WGTACW 1 cut(s) 320
TscAI CASTG 4 cut(s) 118, 206, 307, 532
TseFI GTSAC 2 cut(s) 478, 484
TseI GCWGC 4 cut(s) 189, 334, 413, 425
Tsp45I GTSAC 2 cut(s) 478, 484
TspDTI ATGAA 3 cut(s) 39, 546, 606
TspRI CASTG 4 cut(s) 118, 206, 307, 532
VpaK11BI GGWCC 2 cut(s) 212, 599
XmnI GAANNNNTTC 1 cut(s) 445
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.