Rroxscaffold_7G00183770

protein At4g13200, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012498
Physical Location & Seq
Forward (+)
22961954 .. 22963800
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183770.1

Sequence Viewer

Length: 684 bp
ATGATTAGTGGGGCTTTAGCTTCAGCTTCCCCACAAACTTTTTCTTCTTCATCATCTCTCGTATCTAAACCCAAAACCAGCTGTTACAGCTGTGCTTCCACCTCTGTCAGTGTTGCCAAGCTAAGCTTCTTGGATCTCAAGCTTGAAAGCTTTGGACTTTATGGAGTAAAGGGGTCAAGGAGAGGCAGCAGAGTTCACTGCACTACCGGACCTGGTGAGGGTGATAGCAAGAGTGTTCTGGATGCCTTTTTCTTGGGGAGAGCTCTAGCGGAAGCGCTAAATGAGCGAATTGAGTCCAGTGTCGGTGAGTTTTTGAGTACAATTGGCAGGCTGCAAGCTGAGCAACAAAAGCAAGTAGAGGACTTTCAGAATATTAAAGAAGCCTATTCATTCATTTCTGATTATACACAGGCAGACGTACTGGACAGAGCCAAGAAAGCTAAAGAGAAAGCAGCACGTGAGGCTGCAGAGGCACGAGGAATTGTTTCAAAATCTACTACAGAAAGTGTAACACCAGTCACCGTCACCAATGGTGTTTCGCCAATAACTCCAACCACCACCACCACTGTTGACACTCCTGCTGTCAGTCAACCTTCATCATCTGAGCCGACCCCCACAATAACTGAGAATGAACCAGGTCCTGCCGACGAGGATACATCTTCAGAGGTGCCAATTGATGTTTGA

Protein Analysis

227

Amino Acids

23.88

Weight (kDa)

4.9

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 76 - 124 1.1e-23 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 667
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 141
AcuI CTGAAG 2 cut(s) 6, 645
AcvI CACGTG 1 cut(s) 458
AfaI GTAC 2 cut(s) 319, 420
AfeI AGCGCT 1 cut(s) 276
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 2 cut(s) 146, 489
AjnI CCWGG 2 cut(s) 211, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
Alw21I GWGCWC 1 cut(s) 265
AlwI GGATC 1 cut(s) 141
AlwNI CAGNNNCTG 1 cut(s) 641
Aor51HI AGCGCT 1 cut(s) 276
ApeKI GCWGC 4 cut(s) 186, 331, 452, 464
Asp700I GAANNNNTTC 1 cut(s) 484
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 209, 638
AsuHPI GGTGA 5 cut(s) 227, 233, 317, 511, 517
AvaII GGWCC 2 cut(s) 209, 638
BanI GGYRCC 1 cut(s) 667
BanII GRGCYC 1 cut(s) 265
BauI CACGAG 1 cut(s) 474
BbrPI CACGTG 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 4 cut(s) 198, 318, 451, 464
BciT130I CCWGG 2 cut(s) 213, 636
BciVI GTATCC 1 cut(s) 646
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 2 cut(s) 465, 498
BfoI RGCGCY 1 cut(s) 278
BfuI GTATCC 1 cut(s) 646
BisI GCNGC 4 cut(s) 187, 332, 453, 465
BlpI GCTNAGC 2 cut(s) 122, 339
BlsI GCNGC 4 cut(s) 188, 333, 454, 466
Bme1390I CCNGG 2 cut(s) 213, 636
Bme18I GGWCC 2 cut(s) 209, 638
BmgT120I GGNCC 2 cut(s) 209, 638
BmiI GGNNCC 1 cut(s) 669
BmrFI CCNGG 2 cut(s) 213, 636
BmsI GCATC 1 cut(s) 232
Bpu1102I GCTNAGC 2 cut(s) 122, 339
BpuEI CTTGAG 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 458
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 297, 426, 515
BseBI CCWGG 2 cut(s) 213, 636
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 3 cut(s) 330, 594, 615
BseNI ACTGG 3 cut(s) 297, 426, 515
BseXI GCAGC 4 cut(s) 198, 318, 451, 464
BsgI GTGCAG 1 cut(s) 184
BshNI GGYRCC 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 1 cut(s) 133
Bsp1720I GCTNAGC 2 cut(s) 122, 339
BspACI CCGC 1 cut(s) 269
BspCNI CTCAG 3 cut(s) 331, 595, 616
BspLI GGNNCC 1 cut(s) 669
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 667
BsrI ACTGG 3 cut(s) 297, 426, 515
BssMI GATC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 213, 636
Bst4CI ACNGT 2 cut(s) 523, 568
BstBAI YACGTR 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 329, 336
BstDEI CTNAG 4 cut(s) 122, 339, 603, 624
BstF5I GGATG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 1 cut(s) 136
BstMBI GATC 1 cut(s) 133
BstMWI GCNNNNNNNGC 7 cut(s) 87, 283, 340, 349, 437, 461, 470
BstNI CCWGG 2 cut(s) 213, 636
BstSCI CCNGG 2 cut(s) 211, 634
BstSFI CTRYAG 2 cut(s) 465, 498
BstV1I GCAGC 4 cut(s) 198, 318, 451, 464
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 646
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 4 cut(s) 115, 196, 304, 564
Cac8I GCNNGC 2 cut(s) 329, 336
CaiI CAGNNNCTG 1 cut(s) 641
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 2 cut(s) 209, 638
CsiI ACCWGGT 2 cut(s) 211, 634
Csp6I GTAC 2 cut(s) 318, 419
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviQI GTAC 2 cut(s) 318, 419
DdeI CTNAG 4 cut(s) 122, 339, 603, 624
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 209, 638
Eco47III AGCGCT 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 2 cut(s) 6, 645
Eco72I CACGTG 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 211, 634
EcoT38I GRGCYC 1 cut(s) 265
FaiI YATR 2 cut(s) 162, 405
FalI AAGNNNNNCTT 2 cut(s) 110, 142
Fnu4HI GCNGC 4 cut(s) 187, 332, 453, 465
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 4 cut(s) 187, 332, 453, 465
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 276
GluI GCNGC 4 cut(s) 187, 332, 453, 465
HaeII RGCGCY 1 cut(s) 278
HapII CCGG 1 cut(s) 207
HhaI GCGC 1 cut(s) 277
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 2 cut(s) 571, 590
HindII GTYRAC 2 cut(s) 571, 590
HindIII AAGCTT 3 cut(s) 124, 140, 148
HinfI GANTC 1 cut(s) 293
HpaII CCGG 1 cut(s) 207
HphI GGTGA 5 cut(s) 227, 233, 317, 511, 517
Hpy166II GTNNAC 3 cut(s) 196, 571, 590
Hpy188I TCNGA 4 cut(s) 369, 400, 604, 664
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 3 cut(s) 196, 571, 590
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 1 cut(s) 603
HpyCH4III ACNGT 2 cut(s) 523, 568
HpyCH4IV ACGT 2 cut(s) 417, 457
HpyCH4V TGCA 3 cut(s) 201, 334, 467
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 283, 340, 349, 437, 461, 470
HpyF3I CTNAG 4 cut(s) 122, 339, 603, 624
HpySE526I ACGT 2 cut(s) 417, 457
HspAI GCGC 1 cut(s) 275
Kzo9I GATC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 198, 318, 451, 464
LweI GCATC 1 cut(s) 232
MabI ACCWGGT 2 cut(s) 211, 634
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 417, 457
MaeIII GTNAC 4 cut(s) 83, 508, 517, 523
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 3 cut(s) 36, 39, 651
MfeI CAATTG 2 cut(s) 321, 672
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 4 cut(s) 288, 321, 480, 672
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 575
MnlI CCTC 9 cut(s) 112, 176, 211, 352, 454, 463, 470, 643, 658
MroXI GAANNNNTTC 1 cut(s) 484
MseI TTAA 1 cut(s) 375
MspA1I CMGCKG 2 cut(s) 81, 90
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 213, 636
MunI CAATTG 2 cut(s) 321, 672
MvaI CCWGG 2 cut(s) 213, 636
MwoI GCNNNNNNNGC 7 cut(s) 87, 283, 340, 349, 437, 461, 470
NdeII GATC 1 cut(s) 133
NlaIV GGNNCC 1 cut(s) 669
NmuCI GTSAC 2 cut(s) 517, 523
PdmI GAANNNNTTC 1 cut(s) 484
PkrI GCNGC 4 cut(s) 188, 333, 454, 466
PleI GAGTC 1 cut(s) 301
PmaCI CACGTG 1 cut(s) 458
PmlI CACGTG 1 cut(s) 458
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 458
PpuMI RGGWCCY 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 2 cut(s) 211, 634
PspCI CACGTG 1 cut(s) 458
PspGI CCWGG 2 cut(s) 211, 634
PspN4I GGNNCC 1 cut(s) 669
PspPI GGNCC 2 cut(s) 209, 638
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 2 cut(s) 81, 90
RsaI GTAC 2 cut(s) 319, 420
RsaNI GTAC 2 cut(s) 318, 419
SacI GAGCTC 1 cut(s) 265
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 4 cut(s) 187, 332, 453, 465
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 2 cut(s) 209, 638
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 213, 636
SduI GDGCHC 1 cut(s) 265
SexAI ACCWGGT 2 cut(s) 211, 634
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 2 cut(s) 465, 498
SinI GGWCC 2 cut(s) 209, 638
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 4 cut(s) 288, 321, 480, 672
SsiI CCGC 1 cut(s) 269
SspI AATATT 1 cut(s) 373
SspMI CTAG 1 cut(s) 266
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 2 cut(s) 211, 634
TaaI ACNGT 2 cut(s) 523, 568
TaiI ACGT 2 cut(s) 420, 460
TasI AATT 4 cut(s) 288, 321, 480, 672
TatI WGTACW 1 cut(s) 317
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 4 cut(s) 115, 203, 304, 571
TseFI GTSAC 2 cut(s) 517, 523
TseI GCWGC 4 cut(s) 186, 331, 452, 464
Tsp45I GTSAC 2 cut(s) 517, 523
TspDTI ATGAA 5 cut(s) 39, 378, 382, 585, 645
TspRI CASTG 4 cut(s) 115, 203, 304, 571
VpaK11BI GGWCC 2 cut(s) 209, 638
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.